Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative...Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A （CYP1A）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT）, and glutathione-S-transferase （GST） in crucian carp （Carassius auratus）. The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations $5, $6, and $8 （p 〈 0.05）. SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites （p 〈 0.05）; GPx and CAT mRNA expression levels were also down-regulated at $9 （p 〈 0.05）. In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.展开更多
China calls for turning UNFCCC provisions into concrete actions Never before has climate change been as prominent on the public agenda as it is today.Its rele- vance was highlighted once again when more than 10,000 de...China calls for turning UNFCCC provisions into concrete actions Never before has climate change been as prominent on the public agenda as it is today.Its rele- vance was highlighted once again when more than 10,000 delegates from over 180 countries flocked to Bali early this month to discuss the topic.Environment officials as well as representatives from intergovernmental and nongovernmental organizations gath- ered on the Indonesian island on December 3-14 for the UN Climate Change Conference.展开更多
Pharmacogenomics is the study of the impact of genetic variations or genotypes of individuals on their drug response or drug metabolism. Compared to traditional genomics research,pharmacogenomic research is more close...Pharmacogenomics is the study of the impact of genetic variations or genotypes of individuals on their drug response or drug metabolism. Compared to traditional genomics research,pharmacogenomic research is more closely related to clinical practice. Pharmacogenomic discoveries may effectively assist clinicians and healthcare providers in determining the right drugs and proper dose for each patient, which can help avoid side effects or adverse reactions, and improve the drug therapy. Currently, pharmacogenomic approaches have proven their utility when it comes to the use of cardiovascular drugs, antineoplastic drugs, aromatase inhibitors, and agents used for infectious diseases. The rapid innovation in sequencing technology and genome-wide association studies has led to the development of numerous data resources and dramatically changed the landscape of pharmacogenomic research. Here we describe some of these web resources along with their names, web links, main contents, and our ratings.展开更多
基金supported by the Water Pollution Control and Management(No.2009ZX07528)
文摘Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A （CYP1A）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT）, and glutathione-S-transferase （GST） in crucian carp （Carassius auratus）. The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations $5, $6, and $8 （p 〈 0.05）. SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites （p 〈 0.05）; GPx and CAT mRNA expression levels were also down-regulated at $9 （p 〈 0.05）. In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.
文摘China calls for turning UNFCCC provisions into concrete actions Never before has climate change been as prominent on the public agenda as it is today.Its rele- vance was highlighted once again when more than 10,000 delegates from over 180 countries flocked to Bali early this month to discuss the topic.Environment officials as well as representatives from intergovernmental and nongovernmental organizations gath- ered on the Indonesian island on December 3-14 for the UN Climate Change Conference.
基金supported by the National High Technology R&D Program of China(863 ProgramGrant Nos.2015AA020100 and 2012AA020409)+1 种基金the National Natural Science Foundation of China(Grant No.81201666)the National Scientific-Basic Special Fund(Grant No.2009FY120100)by the Ministry of Science and Technology of China
文摘Pharmacogenomics is the study of the impact of genetic variations or genotypes of individuals on their drug response or drug metabolism. Compared to traditional genomics research,pharmacogenomic research is more closely related to clinical practice. Pharmacogenomic discoveries may effectively assist clinicians and healthcare providers in determining the right drugs and proper dose for each patient, which can help avoid side effects or adverse reactions, and improve the drug therapy. Currently, pharmacogenomic approaches have proven their utility when it comes to the use of cardiovascular drugs, antineoplastic drugs, aromatase inhibitors, and agents used for infectious diseases. The rapid innovation in sequencing technology and genome-wide association studies has led to the development of numerous data resources and dramatically changed the landscape of pharmacogenomic research. Here we describe some of these web resources along with their names, web links, main contents, and our ratings.
