Fuel conversion and clean energy reaction systems involve a variety of catalytic and non-catalytic gas-solid thermochemical reactions.A good understanding of the correct reaction mechanism and kinetics,as well as the ...Fuel conversion and clean energy reaction systems involve a variety of catalytic and non-catalytic gas-solid thermochemical reactions.A good understanding of the correct reaction mechanism and kinetics,as well as the profiles of reaction products,is of great significance to the development,design,and operation of such reaction systems.The micro fluidized bed reaction analysis provides an efficient and reliable method to acquire this essential information with low capital and operating costs,low energy consumption and enhanced safety.This paper provides an overview of the system and its characteristics for the micro fluidized bed reaction analyzer that has been well proven to be a reliable new approach as well as an instrument for characterizing various gas-solid thermochemical reactions.展开更多
AIM: To investigate oxaliplatin-induced severe anaphylactic reactions (SAR) in metastatic colorectal cancer in a retrospective case series analysis and to conduct a systemic literature review. METHODS: During a 6-year...AIM: To investigate oxaliplatin-induced severe anaphylactic reactions (SAR) in metastatic colorectal cancer in a retrospective case series analysis and to conduct a systemic literature review. METHODS: During a 6-year period from 2006 to 2011 at Kaohsiung Veterans General Hospital, a total of 412 patients exposed to oxaliplatin-related chemotherapy were retrospectively reviewed. Relevant Englishlanguage studies regarding life-threatening SAR following oxaliplatin were also reviewed in MEDLINE and PubMed search. RESULTS: Eight patients (1.9%, 8 of 412 cases) were identified. Seven patients were successful resuscitated without any sequelae and one patient expired. We changed the chemotherapy regimen in five patients and rechallenged oxaliplatin use in patient 3. Twenty-three relevant English-language studies with 66 patients were reported. Patients received a median of 10 cycles of oxaliplatin (range, 2 to 29). Most common symptoms were respiratory distress (60%), fever (55%), and hypotension (54%). Three fatal events were reported (4.5%). Eleven patients (16%) of the 66 cases were rechallenged by oxaliplatin. CONCLUSION: SAR must be considered in patients receiving oxaliplatin-related chemotherapy, especially in heavily pretreated patients. Further studies on the mechanism, predictors, preventive methods and management of oxaliplatin-related SAR are recommended.展开更多
Co-carbonization of weakly caking coal and zinc-containing dust to prepare highly reactive ferro-coke and collaboratively recover zinc powder is one of the feasible ways for steel enterprises to recycle zinc-containin...Co-carbonization of weakly caking coal and zinc-containing dust to prepare highly reactive ferro-coke and collaboratively recover zinc powder is one of the feasible ways for steel enterprises to recycle zinc-containing dust.The pyrolysis mass loss behavior of adding blast furnace dust with different zinc contents to different ferro-coke materials was systematically studied by thermogravimetry and differential thermogravimetry analysis,and the kinetic mechanism of pyrolysis-reduction reaction of hybrid briquette was explored.The results of thermogravimetric curve analysis show that the addition of zinc oxide to the sample has no significant effect on the mass loss rate of the sample below 580℃,and the pyrolysis mass loss of zinc oxide mainly occurs between 800 and 1000℃.Kinetic analysis results show that the pyrolysis of zinc-containing samples is controlled by chemical reactions below 580℃.The reaction at 580–700℃ is controlled by the nucleation and growth model,and that above 700℃ is mainly controlled by diffusion.The results of X-ray diffraction analysis show that the pyrolysis process can effectively remove zinc oxide from ferro-coke.展开更多
AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing techn...AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), Polyethylene glycol (PEG) respectively. Then in the presence of a 5′ labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC 42 and HC 49) were sequenced.展开更多
Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environm...Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environmental sample with proton beam from accelerator provides rapid assessment of fluorine contamination. In this paper, 340 keV proton beam induced F 19 (P,αγ) O 16 reaction is performed, measurement of prompt gamma 6130 keV gives fluorine content in the soil and leaves of plants (parasol, cotton and glossy privet), taken from the fluorine polluted area.展开更多
Combining a detailed catalytic surface reaction mechanism with noble metal and promoter elementary reactions, a new three-way catalytic converter(TWC) reaction mechanism is established. Based on the new mechanism, ste...Combining a detailed catalytic surface reaction mechanism with noble metal and promoter elementary reactions, a new three-way catalytic converter(TWC) reaction mechanism is established. Based on the new mechanism, steady condition numerical simulation is carried out, and the change of light-off temperatures and conversion efficiency with various SO2 contents is obtained. By grey relational analysis(GRA), the relational grade between conversion efficiency and SO2 content is obtained. And, the result shows that SO2 content has the most important influence on C3H6 and NOX conversion efficiency. This provides an important reference to the improvement of activity design of TWC, and may provide guidance for the condition design and optimization of TWC.展开更多
AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential ...AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential LNM- associated biomarkers for CRC using quantitative real- time polymerase chain reaction （RT-PCR） analysis. METHODS： Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM （LNM CRC） and without LNM （non-LNM CRC） were assessed in 96 Chinese colorectal carcinoma samples using quantita- tive RT-PCR analysis, Western blotting, and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diaclnostic biomarkers was also examined.RESULTS： TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immuno- histochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramati- cally higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM （90.2% in LNM group vs 22% in non-LNM group, P 〈 0.001）, the advanced tumor node metastasis stage （89.1% in LNM group vs 26.9% in non-LNM group, P 〈 0.001）, the increased 5-year recurrence rate （82.7% in LNM group vs 22.6% in non-LNM group, P 〈 0.001） and the decreased 5-year overall survival rate （48.4% in LNM vs 83.2% in non-LNM group, P 〈 0.001）. Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients （Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028）. CONCLUSION： Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio- marker for LNM and a prognostic factor in CRC. Over- expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients. 2012 Baishideng. All rights reserved展开更多
Hydrogen distribution and content in diamond films deposited by DC arcjetunder gas recycling mode was evaluated by nuclear reaction analysis (NRA). The films werecharacterized using scanning electron microscopy, X-ray...Hydrogen distribution and content in diamond films deposited by DC arcjetunder gas recycling mode was evaluated by nuclear reaction analysis (NRA). The films werecharacterized using scanning electron microscopy, X-ray diffraction and Raman spectrometry. The NRAresults show that the hydrogen content in diamond films was approximately 0.6% (substratetemperature 770℃), and strongly depended on the substrate temperature. It was that the hydrogencontent increased with the increase of the substrate temperature. The possibility of hydrogentrapping in the films was also discussed.展开更多
Recently, non-equilibrium plasma assisted combustion （PAC） has been found to be promising in reducing the ignition delay time in hypersonic propulsion system. NO x produced by non-equilibrium plasma can react with i...Recently, non-equilibrium plasma assisted combustion （PAC） has been found to be promising in reducing the ignition delay time in hypersonic propulsion system. NO x produced by non-equilibrium plasma can react with intermediates during the fuel oxidation process and thereby has influence on the combustion process. In this study, the effects of NO x addition on the ignition process of both the homogeneous ethylene/air mixtures and the non-premixed diffusion layer are examined numerically. The detailed chemistry for ethylene oxidization together with the NO x sub-mechanism is included in the simulation. Reaction path analysis and sensitivity analysis are conducted to give a mechanistic interpretation for the ignition enhancement by NO x addition. It is found that for both the homogenous and non-premixed ignition processes at normal and elevated pressures, NO 2 addition has little influence on the ignition delay time while NO addition can significantly promote the ignition process. The ignition enhancement is found to be caused by the promotion in hydroxyl radical production which quickly oxidizes ethylene. The promotion in hydroxyl radical production by NO addition is achieved in two ways：one is the direct production of OH through the reaction HO2＋NO = NO2＋OH, and the other is the indirect production of OH through the reactions NO＋O2=NO2＋O and C2H4＋O = C2H3＋OH. Moreover, it is found that similar to the homogeneous ignition process, the acceleration of the diffusion layer ignition is also controlled by the reaction HO2＋NO = NO2＋OH.展开更多
Fast atom bombardment mass spectrometry (FAB-MS) is applied to distinguish N- terminal series ions from C-terminal series ions of a peptide by on-probe acetylation, it provides valuable information about the sequence ...Fast atom bombardment mass spectrometry (FAB-MS) is applied to distinguish N- terminal series ions from C-terminal series ions of a peptide by on-probe acetylation, it provides valuable information about the sequence of an unknown peptide. The FAB mass spectra contain a number of characteristic ions at low-mass region in addition to the sequence ions at high-mass region. It was found that the ions below m/z 200 are characteristic of the amino acid composition of the peptide, from which the amino acid composition of the peptide could be estimated. Additionally, mixture analysis is also discussed.展开更多
Developing catalysts capable of efficiently utilizing both visible and near-infrared wavelengths of the solar spectrum for CO_(2)hydrogenation has led to growing interest in reduced TiO_(2)materials.Achieving efficien...Developing catalysts capable of efficiently utilizing both visible and near-infrared wavelengths of the solar spectrum for CO_(2)hydrogenation has led to growing interest in reduced TiO_(2)materials.Achieving efficient long-wavelength solar light harvesting requires a high concentration of oxygen vacancies(O_(V)).However,extensive O_(V)formation can lead to atomic rearrangements within TiO_(X),causing a dispersion of O_(V)throughout the material,as opposed to creating localized and distinct O_(V)sites typical of crystalline TiO_(X),which interact directly with reactants.In this study,we synthesized amorphous black TiO_(X)(AM-TiO_(X))catalysts and thoroughly characterized their surface properties,including acidity and the desorption bond strengths of H_(2)and CO_(2).Density functional theory(DFT)simulations were performed to analyze the hydrogen adsorption profile and structural changes in the material due to O_(V)formation.We found that hydrogen mobility on the surface is restricted due to strong hydrogen bonding.The CO_(2)hydrogenation process was investigated using in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS),enabling the development of a reaction pathway to elucidate the catalyst’s selectivity towards CO and the effect of light irradiation on product formation rates.Notably,m-HCO_(3)^(*)formation was favored,with CO and CH_(4)production proceeding primarily via the formate pathway.To enhance catalyst stability against oxidation during reaction,the surface was decorated with Ru particles.The findings of this study are relevant to catalysts that leverage extensive O_(V)formation as a strategy to extend light responsiveness,as well as to the design of catalysts for CO_(2)hydrogenation to CO.展开更多
To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7...To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci展开更多
基金the financial supports of the Ministry of Science and Technology of People’s Republic of China(2018YFE0103400)the National Natural Science Foundation of China(U1908201).
文摘Fuel conversion and clean energy reaction systems involve a variety of catalytic and non-catalytic gas-solid thermochemical reactions.A good understanding of the correct reaction mechanism and kinetics,as well as the profiles of reaction products,is of great significance to the development,design,and operation of such reaction systems.The micro fluidized bed reaction analysis provides an efficient and reliable method to acquire this essential information with low capital and operating costs,low energy consumption and enhanced safety.This paper provides an overview of the system and its characteristics for the micro fluidized bed reaction analyzer that has been well proven to be a reliable new approach as well as an instrument for characterizing various gas-solid thermochemical reactions.
文摘AIM: To investigate oxaliplatin-induced severe anaphylactic reactions (SAR) in metastatic colorectal cancer in a retrospective case series analysis and to conduct a systemic literature review. METHODS: During a 6-year period from 2006 to 2011 at Kaohsiung Veterans General Hospital, a total of 412 patients exposed to oxaliplatin-related chemotherapy were retrospectively reviewed. Relevant Englishlanguage studies regarding life-threatening SAR following oxaliplatin were also reviewed in MEDLINE and PubMed search. RESULTS: Eight patients (1.9%, 8 of 412 cases) were identified. Seven patients were successful resuscitated without any sequelae and one patient expired. We changed the chemotherapy regimen in five patients and rechallenged oxaliplatin use in patient 3. Twenty-three relevant English-language studies with 66 patients were reported. Patients received a median of 10 cycles of oxaliplatin (range, 2 to 29). Most common symptoms were respiratory distress (60%), fever (55%), and hypotension (54%). Three fatal events were reported (4.5%). Eleven patients (16%) of the 66 cases were rechallenged by oxaliplatin. CONCLUSION: SAR must be considered in patients receiving oxaliplatin-related chemotherapy, especially in heavily pretreated patients. Further studies on the mechanism, predictors, preventive methods and management of oxaliplatin-related SAR are recommended.
基金financially supported by the National Natural Science Foundation of China(No.52274316)the State Key Laboratory of Advanced Metallurgy,University of Science and Technology Beijing(Nos.41620025,41620026,and 41621009)the Interdisciplinary Research Project for Young Teachers of University of Science and Technology Beijing(the Fundamental Research Funds for the Central Universities)(No.FRF-IDRY-20-014).
文摘Co-carbonization of weakly caking coal and zinc-containing dust to prepare highly reactive ferro-coke and collaboratively recover zinc powder is one of the feasible ways for steel enterprises to recycle zinc-containing dust.The pyrolysis mass loss behavior of adding blast furnace dust with different zinc contents to different ferro-coke materials was systematically studied by thermogravimetry and differential thermogravimetry analysis,and the kinetic mechanism of pyrolysis-reduction reaction of hybrid briquette was explored.The results of thermogravimetric curve analysis show that the addition of zinc oxide to the sample has no significant effect on the mass loss rate of the sample below 580℃,and the pyrolysis mass loss of zinc oxide mainly occurs between 800 and 1000℃.Kinetic analysis results show that the pyrolysis of zinc-containing samples is controlled by chemical reactions below 580℃.The reaction at 580–700℃ is controlled by the nucleation and growth model,and that above 700℃ is mainly controlled by diffusion.The results of X-ray diffraction analysis show that the pyrolysis process can effectively remove zinc oxide from ferro-coke.
文摘AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), Polyethylene glycol (PEG) respectively. Then in the presence of a 5′ labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC 42 and HC 49) were sequenced.
文摘Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environmental sample with proton beam from accelerator provides rapid assessment of fluorine contamination. In this paper, 340 keV proton beam induced F 19 (P,αγ) O 16 reaction is performed, measurement of prompt gamma 6130 keV gives fluorine content in the soil and leaves of plants (parasol, cotton and glossy privet), taken from the fluorine polluted area.
基金Project(2009CK2001) supported by the Science & Technology Development Key Program of Hunan Province STA of ChinaProject supported by the Young Teachers Program of Hunan University,China
文摘Combining a detailed catalytic surface reaction mechanism with noble metal and promoter elementary reactions, a new three-way catalytic converter(TWC) reaction mechanism is established. Based on the new mechanism, steady condition numerical simulation is carried out, and the change of light-off temperatures and conversion efficiency with various SO2 contents is obtained. By grey relational analysis(GRA), the relational grade between conversion efficiency and SO2 content is obtained. And, the result shows that SO2 content has the most important influence on C3H6 and NOX conversion efficiency. This provides an important reference to the improvement of activity design of TWC, and may provide guidance for the condition design and optimization of TWC.
基金Supported by The Grants from Shanghai Health Bureau,No.JG1103
文摘AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential LNM- associated biomarkers for CRC using quantitative real- time polymerase chain reaction （RT-PCR） analysis. METHODS： Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM （LNM CRC） and without LNM （non-LNM CRC） were assessed in 96 Chinese colorectal carcinoma samples using quantita- tive RT-PCR analysis, Western blotting, and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diaclnostic biomarkers was also examined.RESULTS： TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immuno- histochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramati- cally higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM （90.2% in LNM group vs 22% in non-LNM group, P 〈 0.001）, the advanced tumor node metastasis stage （89.1% in LNM group vs 26.9% in non-LNM group, P 〈 0.001）, the increased 5-year recurrence rate （82.7% in LNM group vs 22.6% in non-LNM group, P 〈 0.001） and the decreased 5-year overall survival rate （48.4% in LNM vs 83.2% in non-LNM group, P 〈 0.001）. Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients （Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028）. CONCLUSION： Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio- marker for LNM and a prognostic factor in CRC. Over- expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients. 2012 Baishideng. All rights reserved
文摘Hydrogen distribution and content in diamond films deposited by DC arcjetunder gas recycling mode was evaluated by nuclear reaction analysis (NRA). The films werecharacterized using scanning electron microscopy, X-ray diffraction and Raman spectrometry. The NRAresults show that the hydrogen content in diamond films was approximately 0.6% (substratetemperature 770℃), and strongly depended on the substrate temperature. It was that the hydrogencontent increased with the increase of the substrate temperature. The possibility of hydrogentrapping in the films was also discussed.
基金Beijing Municipal Natural Science Foundation(Grant No.3102016)National Natural Science Foundation of China(Grant Nos.50976003,51136005)for funding this work
文摘Recently, non-equilibrium plasma assisted combustion （PAC） has been found to be promising in reducing the ignition delay time in hypersonic propulsion system. NO x produced by non-equilibrium plasma can react with intermediates during the fuel oxidation process and thereby has influence on the combustion process. In this study, the effects of NO x addition on the ignition process of both the homogeneous ethylene/air mixtures and the non-premixed diffusion layer are examined numerically. The detailed chemistry for ethylene oxidization together with the NO x sub-mechanism is included in the simulation. Reaction path analysis and sensitivity analysis are conducted to give a mechanistic interpretation for the ignition enhancement by NO x addition. It is found that for both the homogenous and non-premixed ignition processes at normal and elevated pressures, NO 2 addition has little influence on the ignition delay time while NO addition can significantly promote the ignition process. The ignition enhancement is found to be caused by the promotion in hydroxyl radical production which quickly oxidizes ethylene. The promotion in hydroxyl radical production by NO addition is achieved in two ways：one is the direct production of OH through the reaction HO2＋NO = NO2＋OH, and the other is the indirect production of OH through the reactions NO＋O2=NO2＋O and C2H4＋O = C2H3＋OH. Moreover, it is found that similar to the homogeneous ignition process, the acceleration of the diffusion layer ignition is also controlled by the reaction HO2＋NO = NO2＋OH.
文摘Fast atom bombardment mass spectrometry (FAB-MS) is applied to distinguish N- terminal series ions from C-terminal series ions of a peptide by on-probe acetylation, it provides valuable information about the sequence of an unknown peptide. The FAB mass spectra contain a number of characteristic ions at low-mass region in addition to the sequence ions at high-mass region. It was found that the ions below m/z 200 are characteristic of the amino acid composition of the peptide, from which the amino acid composition of the peptide could be estimated. Additionally, mixture analysis is also discussed.
基金supported by the Second Century Fund(C2F)of Chulalongkorn University,Thailand.
文摘Developing catalysts capable of efficiently utilizing both visible and near-infrared wavelengths of the solar spectrum for CO_(2)hydrogenation has led to growing interest in reduced TiO_(2)materials.Achieving efficient long-wavelength solar light harvesting requires a high concentration of oxygen vacancies(O_(V)).However,extensive O_(V)formation can lead to atomic rearrangements within TiO_(X),causing a dispersion of O_(V)throughout the material,as opposed to creating localized and distinct O_(V)sites typical of crystalline TiO_(X),which interact directly with reactants.In this study,we synthesized amorphous black TiO_(X)(AM-TiO_(X))catalysts and thoroughly characterized their surface properties,including acidity and the desorption bond strengths of H_(2)and CO_(2).Density functional theory(DFT)simulations were performed to analyze the hydrogen adsorption profile and structural changes in the material due to O_(V)formation.We found that hydrogen mobility on the surface is restricted due to strong hydrogen bonding.The CO_(2)hydrogenation process was investigated using in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS),enabling the development of a reaction pathway to elucidate the catalyst’s selectivity towards CO and the effect of light irradiation on product formation rates.Notably,m-HCO_(3)^(*)formation was favored,with CO and CH_(4)production proceeding primarily via the formate pathway.To enhance catalyst stability against oxidation during reaction,the surface was decorated with Ru particles.The findings of this study are relevant to catalysts that leverage extensive O_(V)formation as a strategy to extend light responsiveness,as well as to the design of catalysts for CO_(2)hydrogenation to CO.
基金ThisprojectwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 39970 667)
文摘To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci
