The compactness of plant chromosome and the structures of plant cell wall and cytoplasm pose a great resistance to fluorescence in situ hybridization(FISH),and consequently many new methods for improving spatial resol...The compactness of plant chromosome and the structures of plant cell wall and cytoplasm pose a great resistance to fluorescence in situ hybridization(FISH),and consequently many new methods for improving spatial resolution are being exploited to overcome these problems.However,for plants with small chromosomes like rice and Brassica,there are still many difficulties.In this article a new and effective technique for preparation of extended DNA fibers(EDFs),using a series of treatments to prophase I chromosomes of Brassica oleracea PMCs,is presented.This technique allows longitudinal extension of the chromosomes 30-107 times longer than those of their metaphase counterparts.The length of the extended DNA fibers is between 89μm and 273μm,and the space resolution is 42.8-53.0 kb.Stretching ratios were assessed in a number of FISH experiments with super-stretched chromosomes from meiotic prophase I nuclei ofB.olerecea.Through FISH to EDFs of pachytene chromosomes hybridized in situ with SRK(S-locus receptor kinase)and SPⅡ(S-locus proteinⅡ)probes,for the first time we localized the accurate positions of S-locus and quantitatively analyzed the features of S genes in B.oleracea genome to show all S genes were single-copied.In addition,the length between two linked genes was measured to be about one micron.As a result,the highest space resolution which was about 4 kb was obtained.展开更多
La2/3Sr1/3MnO3/La1.4Sr1.6Mn2O7 composites with arbitrary weight percentage were prepared using a one-step solid-state reaction method. The experimental results demonstrated that addition of K2CO3 during preparation fa...La2/3Sr1/3MnO3/La1.4Sr1.6Mn2O7 composites with arbitrary weight percentage were prepared using a one-step solid-state reaction method. The experimental results demonstrated that addition of K2CO3 during preparation favored the formation of the composites even though the K+ ions were volatilized under the high temperatures of sintering. Full quantitative analysis with the Rietveld method showed that the content of La1.4Sr1.6Mn2O7 phase decreased and the fraction of the La2/3Sr1/3MnO3 phase increased as the a...展开更多
OBJECTIVE:To quantitatively analyze the longitudinal changes of ground‑glass opacity(GGO),consolidation and total lesion in patients infected with severe coronavirus disease 2019(COVID‑19),and its correlation with lab...OBJECTIVE:To quantitatively analyze the longitudinal changes of ground‑glass opacity(GGO),consolidation and total lesion in patients infected with severe coronavirus disease 2019(COVID‑19),and its correlation with laboratory examination results.MATERIALS AND METHODS:All 76 computed tomography(CT)images and laboratory examination results from the admission to discharge of 15 patients confirmed with severe COVID‑19 were reviewed,whereas the GGO volume ratio,consolidation volume ratio,and total lesion volume ratio in different stages were analyzed.The correlations of lesions volume ratio and laboratory examination results were investigated.RESULTS:Four stages were identified based on the degree of lung involvement from day 1 to day 28 after disease onset.GGO was the most common CT manifestation in the four stages.The peak of lung involvement was at around stage 2,and corresponding total lesion volume ratio,GGO volume ratio,and consolidation volume ratio were 17.48(13.44−24.33),12.11(7.34−17.08),and 5.51(2.58−8.58),respectively.Total lesion volume ratio was positively correlated with neutrophil percentage,C‑reactive protein(CRP),high‑sensitivity CRP(Hs‑CRP),procalcitonin,lactate dehydrogenase(LD),and creatine kinase isoenzyme MB(CK‑MB),but negatively correlated with lymphocyte count,lymphocyte percentage,arterial oxygen saturation,and arterial oxygen tension.Consolidation volume ratio was correlated with most above laboratory examination results except Hs‑CRP,LD,and CK‑MB.GGO,however,was only correlated with lymphocyte count.CONCLUSION:CT quantitative parameters could show longitudinal changes well.Total lesion volume ratio and consolidation volume ratio are well correlated with laboratory examination results,suggesting that CT quantitative parameters may be an effective tool to reflect the changes in the condition.展开更多
The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive...The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.60140646)the Natural Science Foundation of Chongqing(No.7914).
文摘The compactness of plant chromosome and the structures of plant cell wall and cytoplasm pose a great resistance to fluorescence in situ hybridization(FISH),and consequently many new methods for improving spatial resolution are being exploited to overcome these problems.However,for plants with small chromosomes like rice and Brassica,there are still many difficulties.In this article a new and effective technique for preparation of extended DNA fibers(EDFs),using a series of treatments to prophase I chromosomes of Brassica oleracea PMCs,is presented.This technique allows longitudinal extension of the chromosomes 30-107 times longer than those of their metaphase counterparts.The length of the extended DNA fibers is between 89μm and 273μm,and the space resolution is 42.8-53.0 kb.Stretching ratios were assessed in a number of FISH experiments with super-stretched chromosomes from meiotic prophase I nuclei ofB.olerecea.Through FISH to EDFs of pachytene chromosomes hybridized in situ with SRK(S-locus receptor kinase)and SPⅡ(S-locus proteinⅡ)probes,for the first time we localized the accurate positions of S-locus and quantitatively analyzed the features of S genes in B.oleracea genome to show all S genes were single-copied.In addition,the length between two linked genes was measured to be about one micron.As a result,the highest space resolution which was about 4 kb was obtained.
基金Project supported by the High Technology Research and Development Program of China (2007AA03Z116)Natural Science Foundation of He-bei Province (E2008000194) Science Foundation of Hebei Education Department (2009133)
文摘La2/3Sr1/3MnO3/La1.4Sr1.6Mn2O7 composites with arbitrary weight percentage were prepared using a one-step solid-state reaction method. The experimental results demonstrated that addition of K2CO3 during preparation favored the formation of the composites even though the K+ ions were volatilized under the high temperatures of sintering. Full quantitative analysis with the Rietveld method showed that the content of La1.4Sr1.6Mn2O7 phase decreased and the fraction of the La2/3Sr1/3MnO3 phase increased as the a...
基金funded by the Novel Coronavirus Pneumonia Emergency Clinical Research Project of Chongqing Medical University(30).
文摘OBJECTIVE:To quantitatively analyze the longitudinal changes of ground‑glass opacity(GGO),consolidation and total lesion in patients infected with severe coronavirus disease 2019(COVID‑19),and its correlation with laboratory examination results.MATERIALS AND METHODS:All 76 computed tomography(CT)images and laboratory examination results from the admission to discharge of 15 patients confirmed with severe COVID‑19 were reviewed,whereas the GGO volume ratio,consolidation volume ratio,and total lesion volume ratio in different stages were analyzed.The correlations of lesions volume ratio and laboratory examination results were investigated.RESULTS:Four stages were identified based on the degree of lung involvement from day 1 to day 28 after disease onset.GGO was the most common CT manifestation in the four stages.The peak of lung involvement was at around stage 2,and corresponding total lesion volume ratio,GGO volume ratio,and consolidation volume ratio were 17.48(13.44−24.33),12.11(7.34−17.08),and 5.51(2.58−8.58),respectively.Total lesion volume ratio was positively correlated with neutrophil percentage,C‑reactive protein(CRP),high‑sensitivity CRP(Hs‑CRP),procalcitonin,lactate dehydrogenase(LD),and creatine kinase isoenzyme MB(CK‑MB),but negatively correlated with lymphocyte count,lymphocyte percentage,arterial oxygen saturation,and arterial oxygen tension.Consolidation volume ratio was correlated with most above laboratory examination results except Hs‑CRP,LD,and CK‑MB.GGO,however,was only correlated with lymphocyte count.CONCLUSION:CT quantitative parameters could show longitudinal changes well.Total lesion volume ratio and consolidation volume ratio are well correlated with laboratory examination results,suggesting that CT quantitative parameters may be an effective tool to reflect the changes in the condition.
基金support from the Program for Young Innovative Research Team in China University of Political Science and Law.
文摘The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.
