Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were ...Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were received from January 2021 to January 2022, and standardized grouping operation was carried out randomly. The control group applied routine puncture technique to 100 cases included, and the observation group applied detail optimized puncture technique to 100 cases included. The pain score, puncture condition, adverse condition and family satisfaction were compared between groups. Results: the scores of the former group were lower than that of the latter group (P < 0.05). The success rate of one-time puncture was 94.00% in the observation group and 76.00% in the control group. The former group was higher than the latter group, and the puncture time in the former group was lower (P < 0.05);the adverse situation of the children included in the observation group was evaluated as 2.00%, and that of the control group was evaluated as 14.00%. The former group was lower than the latter group (P < 0.05);the satisfaction of the family members of the children included in the observation group was 100.00%, and the satisfaction of the family members of the control group was 90.00%. The former group was higher than the latter group (P < 0.05). Conclusion: the implementation of detail optimization puncture technique in scalp vein infusion with one-time scalp needle puncture can improve the success rate of one-time puncture, reduce the pain of children and make the family satisfied.展开更多
BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine...BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.展开更多
文摘Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were received from January 2021 to January 2022, and standardized grouping operation was carried out randomly. The control group applied routine puncture technique to 100 cases included, and the observation group applied detail optimized puncture technique to 100 cases included. The pain score, puncture condition, adverse condition and family satisfaction were compared between groups. Results: the scores of the former group were lower than that of the latter group (P < 0.05). The success rate of one-time puncture was 94.00% in the observation group and 76.00% in the control group. The former group was higher than the latter group, and the puncture time in the former group was lower (P < 0.05);the adverse situation of the children included in the observation group was evaluated as 2.00%, and that of the control group was evaluated as 14.00%. The former group was lower than the latter group (P < 0.05);the satisfaction of the family members of the children included in the observation group was 100.00%, and the satisfaction of the family members of the control group was 90.00%. The former group was higher than the latter group (P < 0.05). Conclusion: the implementation of detail optimization puncture technique in scalp vein infusion with one-time scalp needle puncture can improve the success rate of one-time puncture, reduce the pain of children and make the family satisfied.
基金the Chongqing Talent Plan“Contract System”Project,No.cstc2022ycjh-bgzxm0137Natural Science Foundation of Chongqing,No.CSTB2024NSCQ-MSX0003the Ethics Committee of Chongqing General Hospital.The ethics review number:No.KY S2022-045-01.
文摘BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.
