Domain of unknown function 1644(DUF1644)is a highly conserved amino acid sequence motif present only in plants.Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate res...Domain of unknown function 1644(DUF1644)is a highly conserved amino acid sequence motif present only in plants.Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate responses to abiotic stress in rice.Here we present our discovery of the role of Os.SIDP366,a member of the DUF1644 gene family,in response to drought and salinity stresses in rice.Transgenic rice plants overexpressing OsSIDP366 showed enhanced drought and salinity tolerance and reduced water loss as compared to that in the control,whereas plants with downregulated OsSIDP366 expression levels using RNA interference(RNAi)were more sensitive to salinity and drought treatments.The sensitivity to abscisic acid(ABA)treatment was not changed in OsStDP366-overexpressing plants,and OsSIDP366 expression was not affected in ABA-deficient mutants.Subcellular localization analysis revealed that OsSIDP366 is presented in the cytoplasmic foci that colocalized with protein markers for both processing bodies(PBs)and stress granules(SGs)in rice protoplasts.Digital gene expression(DGE)profile analysis indicated that stress-related genes such as SNACl,OsHAK5 and PRs were upregulated in OsSIDP366-overexpressing plants.These results suggest that OsSIDP366 may function as a regulator of the PBs/SGs and positively regulate salt and drought resistance in rice.展开更多
microRNAs(miRNAs)are identified as a class of non-protein regulators and a new source for broad control of gene expression in eukaryotes.The past years have witnessed substantial progress in understanding miRNA functi...microRNAs(miRNAs)are identified as a class of non-protein regulators and a new source for broad control of gene expression in eukaryotes.The past years have witnessed substantial progress in understanding miRNA functions and mechanisms,although a few controversies remain.Various hypotheses and models have been suggested for the mechanisms of miRNA repression,including translational inhibition at the level of initiation or elongation,rapid degradation of the nascent peptide,mRNA degradation,and mRNA sequestration into P bodies(processing bodies)and SGs(stress granules)for degradation or/and storage.Recently,some noncanonical miRNA regulation,such as miRNA activation and de-repression of miRNA inhibition,have been uncovered.This review discusses some recent advances about how miRNAs regulate their targets and various modes of miRNA function.展开更多
基金supported by Prophase Project of National Key Basic Research Program of China(2012CB126312)Science and Technology Foundation of Guizhou Province of China([2012]2277)
文摘Domain of unknown function 1644(DUF1644)is a highly conserved amino acid sequence motif present only in plants.Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate responses to abiotic stress in rice.Here we present our discovery of the role of Os.SIDP366,a member of the DUF1644 gene family,in response to drought and salinity stresses in rice.Transgenic rice plants overexpressing OsSIDP366 showed enhanced drought and salinity tolerance and reduced water loss as compared to that in the control,whereas plants with downregulated OsSIDP366 expression levels using RNA interference(RNAi)were more sensitive to salinity and drought treatments.The sensitivity to abscisic acid(ABA)treatment was not changed in OsStDP366-overexpressing plants,and OsSIDP366 expression was not affected in ABA-deficient mutants.Subcellular localization analysis revealed that OsSIDP366 is presented in the cytoplasmic foci that colocalized with protein markers for both processing bodies(PBs)and stress granules(SGs)in rice protoplasts.Digital gene expression(DGE)profile analysis indicated that stress-related genes such as SNACl,OsHAK5 and PRs were upregulated in OsSIDP366-overexpressing plants.These results suggest that OsSIDP366 may function as a regulator of the PBs/SGs and positively regulate salt and drought resistance in rice.
基金Supported by the National Basic Research Program of China(Grant No.2005 CB 724603)the National Natural Science Foundation of China(Grant No.30770474)
文摘microRNAs(miRNAs)are identified as a class of non-protein regulators and a new source for broad control of gene expression in eukaryotes.The past years have witnessed substantial progress in understanding miRNA functions and mechanisms,although a few controversies remain.Various hypotheses and models have been suggested for the mechanisms of miRNA repression,including translational inhibition at the level of initiation or elongation,rapid degradation of the nascent peptide,mRNA degradation,and mRNA sequestration into P bodies(processing bodies)and SGs(stress granules)for degradation or/and storage.Recently,some noncanonical miRNA regulation,such as miRNA activation and de-repression of miRNA inhibition,have been uncovered.This review discusses some recent advances about how miRNAs regulate their targets and various modes of miRNA function.
