Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The functio...Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.展开更多
传统的信号检测算法基于奈奎斯特采样定理来实现,这对于带宽极宽的超宽带(ultra-wideband,UWB)信号而言由于要求采样速率过高而很难用硬件去实现。为此,本文研究了基于压缩感知(compressive sensing,CS)的脉冲超宽带(impulse radio UWB,...传统的信号检测算法基于奈奎斯特采样定理来实现,这对于带宽极宽的超宽带(ultra-wideband,UWB)信号而言由于要求采样速率过高而很难用硬件去实现。为此,本文研究了基于压缩感知(compressive sensing,CS)的脉冲超宽带(impulse radio UWB,IR-UWB)信号检测问题,利用IR-UWB信号在时域上的稀疏特性,设计了一种基于压缩感知的IR-UWB信号检测框架,在此基础上提出了一种自适应加权正交匹配追踪检测算法。仿真结果表明,新算法不仅能够通过远少于奈奎斯特定理所要求的采样速率检测出IR-UWB信号,而且与基于匹配追踪的压缩感知检测算法相比,新算法在低信噪比的情况下对IR-UWB信号的检测效果更佳。展开更多
文摘Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.
文摘传统的信号检测算法基于奈奎斯特采样定理来实现,这对于带宽极宽的超宽带(ultra-wideband,UWB)信号而言由于要求采样速率过高而很难用硬件去实现。为此,本文研究了基于压缩感知(compressive sensing,CS)的脉冲超宽带(impulse radio UWB,IR-UWB)信号检测问题,利用IR-UWB信号在时域上的稀疏特性,设计了一种基于压缩感知的IR-UWB信号检测框架,在此基础上提出了一种自适应加权正交匹配追踪检测算法。仿真结果表明,新算法不仅能够通过远少于奈奎斯特定理所要求的采样速率检测出IR-UWB信号,而且与基于匹配追踪的压缩感知检测算法相比,新算法在低信噪比的情况下对IR-UWB信号的检测效果更佳。
