Following over 20 years of research,a direct measurement of the QGP temperature has been achieved at Relativistic Heavy-Ion Collider(RHIC),free from the blue-shift effect and contamination from strong interactions.Thi...Following over 20 years of research,a direct measurement of the QGP temperature has been achieved at Relativistic Heavy-Ion Collider(RHIC),free from the blue-shift effect and contamination from strong interactions.This viewpoint discusses a recent measurement of the QGP temperature at different stages at the Solenoidal Tracker at RHIC(STAR),which used e^(+)e^(-)pairs as penetrating probes.展开更多
AS the ancient capital of a number of dynasties, the city of Beijing is filled with many scenic spots and historical sites. Besides the Temple of Heaven, Beihai Park, Forbidden City, Summer Palace and so on, there is ...AS the ancient capital of a number of dynasties, the city of Beijing is filled with many scenic spots and historical sites. Besides the Temple of Heaven, Beihai Park, Forbidden City, Summer Palace and so on, there is Guozijian Street, which has been a quiet place in recent decades. Recently, however, it has become more and more famous, attracting visits from foreigners and overseas Chinese. About 10 meters wide展开更多
Precise control and measurement of nanoparticles using low-power optical tweezers are pivotal for advancing single-particle analysis,nanoscale sensing,and energy transport research.In this work,we present the tipassis...Precise control and measurement of nanoparticles using low-power optical tweezers are pivotal for advancing single-particle analysis,nanoscale sensing,and energy transport research.In this work,we present the tipassisted nanoparticle capture system that simultaneously achieves localized temperature probing and nanoparticle trapping,significantly lowering the required laser power input.Unlike traditional metal-tip plasmonic techniques that predominantly rely on intense electric field gradients,our approach employs a silicon nanotip under resonant laser excitation,uniquely integrating optical forces,thermophoretic forces,and interatomic interactions for stable nanoparticle confinement.This synergistic collaboration mechanism enables approximately a 42%reduction in laser power density compared to conventional bowtie nanoaperture methods.This experimental method achieved direct and simultaneous Raman-based measurements of localized thermal dynamics,providing new insights into nanoscale thermodynamics during optical trapping.Additionally,the silicon nanotip demonstrates reduced thermal transport due to its confined nanoscale geometry,aligning closely with our theoretical predictions.Our integrated strategy of efficient nanoparticle manipulation coupled with precise thermal probing not only enhances overall energy efficiency but also broadens the scope of potential applications in cutting-edge nanoscience and nanotechnology.展开更多
China’s Einstein Probe(EP)astronomical satellite has,for the first time in human history,detected soft X-ray signals from an explosion in the early universe,opening a new window for research into the young cosmos.The...China’s Einstein Probe(EP)astronomical satellite has,for the first time in human history,detected soft X-ray signals from an explosion in the early universe,opening a new window for research into the young cosmos.The findings were published in the journal Nature Astronomy on January 23,2025.The Wide-field X-ray Telescope onboard the EP satellite observed a faint pulse signal from a burst event in the soft X-ray band on March 15,2024.This burst,designated as EP240315a,exhibited rapid brightness fluctuations and persisted for over 17 minutes before gradually fading away.Subsequent observations by telescopes from other countries confirmed that this burst originated from the distant early universe.展开更多
We demonstrate an all-optical technique for in situ monitoring of strong-field ionization(SFI)dynamics.The method relies on coherent scattering of a probe pulse from a transient plasma grating(TPG)formed by two interf...We demonstrate an all-optical technique for in situ monitoring of strong-field ionization(SFI)dynamics.The method relies on coherent scattering of a probe pulse from a transient plasma grating(TPG)formed by two interfering femtosecond pulses in a gas target.The diffracted signal provides a real-time,relative measure of the ionization yield.We demonstrate the method’s sensitivity by resolving the attosecond-scale coherent control of the ionization rate.Unlike conventional diagnostics that directly measure charged particles,our ensemble-based optical method is non-invasive and particularly suited for non-vacuum environments.To demonstrate its utility,we apply the technique to investigate the relationship between ionization and terahertz(THz)generation in a two-color(ω+2ω)field.We measure a phase offset of(0.18±0.05)π between the maxima of the ionization yield and the THz emission.This result provides a quantitative benchmark for theoretical models and establishes the TPG technique as a practical tool for probing SFI dynamics in ensemble systems.展开更多
To judge the current economic situation,it is acceptable that the productionand exports/imports data counted in RMB or dollars are used to comparewith the preceding year or month,while the price changes in the sameper...To judge the current economic situation,it is acceptable that the productionand exports/imports data counted in RMB or dollars are used to comparewith the preceding year or month,while the price changes in the sameperiod,represented by the varying of CPI and PPI,are also reported.China’sexports and imports figures,described in US dollars,have reported a doubledigit drop in the past months of this year.But,if probing into the actualtrade amount,it can be found that the imports amount of展开更多
In natural aquatic ecosystems,algal-derived organic carbon(AOC)often coexists with exogenous organic carbon(EOC).Microbial utilization of these distinct carbon sources affects carbon flux and transformation in water c...In natural aquatic ecosystems,algal-derived organic carbon(AOC)often coexists with exogenous organic carbon(EOC).Microbial utilization of these distinct carbon sources affects carbon flux and transformation in water column and algal growth.Microcystis blooms significantly increase AOC levels in water,but the microbial transformation process of Microcystis-derived AOC in the presence of EOC remain poorly understood.We conducted a simulated experiment by introducing^(13)C-sodium bicarbonate and^(13)C-glucose as substrates for indoor simulation of non-axenic Microcystis aeruginosa(M.aeruginosa)populations in a sealed system.The microbial transformation processes of AOC and EOC and their effects on M.aeruginosa growth were investigated.Results demonstrated that the addition of glucose accelerated M.aeruginosa growth and significantly increased their biomass.During the experiment,as the particulate organic carbon and nitrogen content increased,the concentrations of CO_(2)and N_(2)O were gradually decreased,while the concentration of CH4 were gradually increased.Significant differences were observed in the microbial processes involved in the uptake of AOC and EOC.Bacteria involved in AOC transformation throughout the growth period were dominated by Proteobacteria,Gemmatimonadota,Actinobacteriota,Bacteroidota,Acidobacteriota,and Firmicutes.The bacteria involved in EOC transformation were dominated by Proteobacteria,Actinobacteriota,Firmicutes,Cyanobacteria,Armatimonadota,and Bacteroidota.Linear discriminant analysis Effect Size(LEfSe)analysis revealed Massilia and Akkermansia as biomarkers involved in AOC transformation,while Ligilactobacillus was associated with EOC transformation.These findings provide valuable insights into the effects of EOC on algae-bacteria interaction,and on the dynamics of carbon and nitrogen cycling among M.aeruginosa and its associated bacteria.展开更多
The research presented in this paper aimed to analyze the evolution of fractures in strata in relation to the progress of longwall mining.To achieve this objective,an introscopic probe equipped with a highresolution c...The research presented in this paper aimed to analyze the evolution of fractures in strata in relation to the progress of longwall mining.To achieve this objective,an introscopic probe equipped with a highresolution camera was developed,along with additional equipment that enables continuous recording of the internal surfaces of drainage boreholes exceeding 100 m in depth.The probe was utilized to test two methane drainage boreholes in the Z-3b longwall,which operates within the 501/3 coal seam of the Jankowice mine in Poland.Automatic image analysis methods were applied to evaluate the recorded images,based on a newly developed classificationsystem for fractures categorized by size and number.The results were compared with an analysis of changes in the methane capture rate from the drainage boreholes,which correlated with longwall mining progress.A strong correlation was observed between the number of fractures and the lithology of the strata layers.The largest number of fractures and their evolution were recorded in the coal layers,followed by the shale layers,while the sandstone layers exhibited the least number of fractures.Based on parallel measurements of the methane capture rate from the drainage boreholes during the progress of longwall mining,the extent of the strata's fracture zone was determined to range from 6 m to 36 m.Within the fracture zone,the strata are highly fractured,which leads to an increase in methane emissions through seepage and diffusion processes.展开更多
Schizophrenia(SCZ) is a severe mental disorder with an unclear pathogenesis.Increasing evidence suggests that oxidative stress(OS) may contribute to the neuropathological processes underlying SCZ.Biothiols,key endogen...Schizophrenia(SCZ) is a severe mental disorder with an unclear pathogenesis.Increasing evidence suggests that oxidative stress(OS) may contribute to the neuropathological processes underlying SCZ.Biothiols,key endogenous antioxidants,have been proposed as potential biomarkers for the disease.However,due to the presence of the blood-brain barrier(BBB),fluorescent probes are rarely used to image biothiols in the brain of SCZ models.In this study,a series of fluorescent probes for biothiols were developed using dicyanoisophorone derivatives as fluorophores known for their excellent optical properties,and carboxylic esters as recognition units.A parallel synthesis and rapid screening strategy was employed to construct and optimize these probes.By introducing trifluoromethyl and benzothiazole groups into the fluorophore,the emission wavelength was successfully shifted into the near-infrared region.Additionally,various trifluoromethyl-substituted aromatic and nitrogen heterocyclic compounds were incorporated to optimize the carboxylic esters,thereby improving the probes' reactivity and lipophilicity.Systematic evaluation of the physicochemical characteristics,and optical performance led to the identification of DCIBT-11 as the most promising candidate.DCI-BT-11 demonstrated excellent BBB permeability and a good response to biothiols both in vitro and in vivo.Notably,DCI-BT-11 was used for the first time to visualize biothiol flux and assess the therapeutic effects of the antioxidant N-acetylcysteine(NAC) in the brains of SCZ mouse models,offering new insights into the role of OS in the pathogenesis and treatment of SCZ.展开更多
Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of...Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of multiple RSSs significant for evaluating ferroptosis therapy.However,the traditional techniques,including fluorescent(FL) imaging and electrospray ionization-based mass spectrometry(MS) detection,cannot achieve the discrimination of different RSSs.Herein,simultaneous MS detection of multiple RSSs,including cysteine(Cys),homocysteine(Hcy),glutathione(GSH) and hydrogen sulfide(H_(2)S),was obtained upon enhancing ionization efficiency by a fluorescent probe(NBD-O-1).Based on the interaction between NBD-O-1 and RSSs,the complex of RSSs with a fragment of NBD-O-1 can be generated,which can be easily ionized for MS detection in the negative mode.Therefore,the intracellular RSSs can be well detected upon the incubation of He La cells with the probe of NBD-O-1,exhibiting the total RSS levels by the FL imaging and further providing expression of each RSS by enhanced MS detection.Furthermore,the RSSs during ferroptosis in He La cells have been evaluated using the present strategy,demonstrating the potential for ferroptosis examinations.This work has made an unconventional application of a fluorescent probe to enhance the detection of multiple RSSs by MS,providing significant molecular information for addressing the ferroptosis mechanism.展开更多
Cell-selective fluorescent probes have emerged as essential tools for live-cell imaging,enabling the differentiation of specific cell types within complex biological systems.Unlike traditional antibody-based methods t...Cell-selective fluorescent probes have emerged as essential tools for live-cell imaging,enabling the differentiation of specific cell types within complex biological systems.Unlike traditional antibody-based methods that target extracellular proteins,small-molecule probes can access intracellular environments and exploit diverse biochemical features for selective retention or activation.This perspective categorizes the mechanisms of cell selectivity into five principal strategies:Protein-oriented,carbohydrate-oriented,lipid-oriented,gating-oriented,and metabolism-oriented live-cell distinctions.Each class capitalizes on a unique cellular trait ranging from protein expression and membrane composition to transporter activity and metabolic enzyme presence.We discuss representative examples of each mechanism,outline a decision-tree workflow for elucidating a new probe's mode of action,and highlight how understanding these mechanisms is critical for both basic biological research and therapeutic probe design.Looking ahead,the development of such mechanism-informed cell-specific probes holds promise for advancing precision cell targeting in biomedical applications.展开更多
Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewa...Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.展开更多
The study of target proteins is crucial for understanding molecular interactions and developing analytical platforms,therapeutic agents and functional tools.Herein,we present a novel nanoplatform activated by near-inf...The study of target proteins is crucial for understanding molecular interactions and developing analytical platforms,therapeutic agents and functional tools.Herein,we present a novel nanoplatform activated by near-infrared(NIR) light for triple-modal proteins study,which enabling target protein labeling,enrichment and visualization.Azido-naphthalimide-coated upconversion nanoparticles(UCNPs) serve as NIR light-responsive nanoplatforms,showing promising applications in studying interactions between various bioactive molecules and proteins in living systems.Under NIR light irradiation,azido-naphthalimides are activated by ultraviolet(UV) and blue light emitted from UCNPs and the resulting amino-naphthalimides intermediate not only crosslink nearby target proteins but also enable imaging performance.We demonstrate that this nanoplatform is capable of selective protein labeling and imaging in complex protein environments,achieving specific labeling and imaging of both intracellular and extracellular proteins in mammalian cells as well as bacteria.Furthermore,in vivo protein labeling has been achieved using this novel NIR light-activatable nanoplatform.This technique will open new avenues for discoveries and mechanistic interrogation in chemical biology.展开更多
The detection of amino acid enantiomers holds significant importance in biomedical,chemical,food,and other fields.Traditional chiral recognition methods using fluorescent probes primarily rely on fluorescence intensit...The detection of amino acid enantiomers holds significant importance in biomedical,chemical,food,and other fields.Traditional chiral recognition methods using fluorescent probes primarily rely on fluorescence intensity changes,which can compromise accuracy and repeatability.In this study,we report a novel fluorescent probe(R)-Z1 that achieves effective enantioselective recognition of chiral amino acids in water by altering emission wavelengths(>60 nm).This water-soluble probe(R)-Z1 exhibits cyan or yellow-green luminescence upon interaction with amino acid enantiomers,enabling reliable chiral detection of 14 natural amino acids.It also allows for the determination of enantiomeric excess through monitoring changes in luminescent color.Additionally,a logic operation with two inputs and three outputs was constructed based on these optical properties.Notably,amino acid enantiomers were successfully detected via dual-channel analysis at both the food and cellular levels.This study provides a new dynamic luminescence-based tool for the accurate sensing and detection of amino acid enantiomers.展开更多
To address the challenge of low survival rates and limited data collection efficiency in current virtual probe deployments,which results from anomaly detection mechanisms in location-based service(LBS)applications,thi...To address the challenge of low survival rates and limited data collection efficiency in current virtual probe deployments,which results from anomaly detection mechanisms in location-based service(LBS)applications,this paper proposes a novel virtual probe deployment method based on user behavioral feature analysis.The core idea is to circumvent LBS anomaly detection by mimicking real-user behavior patterns.First,we design an automated data extraction algorithm that recognizes graphical user interface(GUI)elements to collect spatio-temporal behavior data.Then,by analyzing the automatically collected user data,we identify normal users’spatio-temporal patterns and extract their features such as high-activity time windows and spatial clustering characteristics.Subsequently,an antidetection scheduling strategy is developed,integrating spatial clustering optimization,load-balanced allocation,and time window control to generate probe scheduling schemes.Additionally,a self-correction mechanism based on an exponential backoff strategy is implemented to rectify anomalous behaviors andmaintain system stability.Experiments in real-world environments demonstrate that the proposed method significantly outperforms baseline methods in terms of both probe ban rate and task completion rate,while maintaining high time efficiency.This study provides a more reliable and clandestine solution for geosocial data collection and lays the foundation for building more robust virtual probe systems.展开更多
Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the q...Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.展开更多
Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particu...Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particular spiritual world, merit and drawback of her character, her love value and conflicts about her thought. Analyzing into internal factors for Anna 's tragedy would offer further appreciation to readers.展开更多
Nanoemulsions(NEs)and nanosuspensions(NSs)show great potential in enhancing the ocular bioavailability of therapeutics through topical delivery.However,transocular fate of intact NEs and NSs is still inconclusive.In t...Nanoemulsions(NEs)and nanosuspensions(NSs)show great potential in enhancing the ocular bioavailability of therapeutics through topical delivery.However,transocular fate of intact NEs and NSs is still inconclusive.In this study,an aggregation-caused quenching fluorescent probe is used to track precorneal retention and transocular transportation of intact NEs and NSs,while coumarin 6 is used to mimick the cargo.NEs show superior precorneal retention to NSs.Both the two types of nanocarriers can permeate into but not across the cornea.The smaller NEs(100 nm)permeate better into the cornea than the bigger ones(210 nm).Nanocarriers in the cornea serves as depots.The released cargo molecules can penetrate across the cornea and diffuse into the lens.Moreover,the conjunctiva-scleral route may be potential to deliver drugs to the back of the eye,In conclusion,the study provides useful tools and information in the field of transocular transportation of nanoparticles.展开更多
文摘Following over 20 years of research,a direct measurement of the QGP temperature has been achieved at Relativistic Heavy-Ion Collider(RHIC),free from the blue-shift effect and contamination from strong interactions.This viewpoint discusses a recent measurement of the QGP temperature at different stages at the Solenoidal Tracker at RHIC(STAR),which used e^(+)e^(-)pairs as penetrating probes.
文摘AS the ancient capital of a number of dynasties, the city of Beijing is filled with many scenic spots and historical sites. Besides the Temple of Heaven, Beihai Park, Forbidden City, Summer Palace and so on, there is Guozijian Street, which has been a quiet place in recent decades. Recently, however, it has become more and more famous, attracting visits from foreigners and overseas Chinese. About 10 meters wide
基金supported by the National Natural Science Foundation of China(Grant No.52206107)the National Key R&D Program of China(Grant No.2023YFE0120200)。
文摘Precise control and measurement of nanoparticles using low-power optical tweezers are pivotal for advancing single-particle analysis,nanoscale sensing,and energy transport research.In this work,we present the tipassisted nanoparticle capture system that simultaneously achieves localized temperature probing and nanoparticle trapping,significantly lowering the required laser power input.Unlike traditional metal-tip plasmonic techniques that predominantly rely on intense electric field gradients,our approach employs a silicon nanotip under resonant laser excitation,uniquely integrating optical forces,thermophoretic forces,and interatomic interactions for stable nanoparticle confinement.This synergistic collaboration mechanism enables approximately a 42%reduction in laser power density compared to conventional bowtie nanoaperture methods.This experimental method achieved direct and simultaneous Raman-based measurements of localized thermal dynamics,providing new insights into nanoscale thermodynamics during optical trapping.Additionally,the silicon nanotip demonstrates reduced thermal transport due to its confined nanoscale geometry,aligning closely with our theoretical predictions.Our integrated strategy of efficient nanoparticle manipulation coupled with precise thermal probing not only enhances overall energy efficiency but also broadens the scope of potential applications in cutting-edge nanoscience and nanotechnology.
文摘China’s Einstein Probe(EP)astronomical satellite has,for the first time in human history,detected soft X-ray signals from an explosion in the early universe,opening a new window for research into the young cosmos.The findings were published in the journal Nature Astronomy on January 23,2025.The Wide-field X-ray Telescope onboard the EP satellite observed a faint pulse signal from a burst event in the soft X-ray band on March 15,2024.This burst,designated as EP240315a,exhibited rapid brightness fluctuations and persisted for over 17 minutes before gradually fading away.Subsequent observations by telescopes from other countries confirmed that this burst originated from the distant early universe.
基金supported by the NUDT Science Foundation for Indigenous Innovation(Grant No.24-ZZCX-ZXGC-15)the National Natural Science Foundation of China(Grant Nos.12234020,12450403,and 12374263)。
文摘We demonstrate an all-optical technique for in situ monitoring of strong-field ionization(SFI)dynamics.The method relies on coherent scattering of a probe pulse from a transient plasma grating(TPG)formed by two interfering femtosecond pulses in a gas target.The diffracted signal provides a real-time,relative measure of the ionization yield.We demonstrate the method’s sensitivity by resolving the attosecond-scale coherent control of the ionization rate.Unlike conventional diagnostics that directly measure charged particles,our ensemble-based optical method is non-invasive and particularly suited for non-vacuum environments.To demonstrate its utility,we apply the technique to investigate the relationship between ionization and terahertz(THz)generation in a two-color(ω+2ω)field.We measure a phase offset of(0.18±0.05)π between the maxima of the ionization yield and the THz emission.This result provides a quantitative benchmark for theoretical models and establishes the TPG technique as a practical tool for probing SFI dynamics in ensemble systems.
文摘To judge the current economic situation,it is acceptable that the productionand exports/imports data counted in RMB or dollars are used to comparewith the preceding year or month,while the price changes in the sameperiod,represented by the varying of CPI and PPI,are also reported.China’sexports and imports figures,described in US dollars,have reported a doubledigit drop in the past months of this year.But,if probing into the actualtrade amount,it can be found that the imports amount of
基金Supported by the Jiangsu Provincial Science and Technology Planning Project(No.BK20231516)the National Natural Science Foundation of China(Nos.42293264,32371606,31971449)。
文摘In natural aquatic ecosystems,algal-derived organic carbon(AOC)often coexists with exogenous organic carbon(EOC).Microbial utilization of these distinct carbon sources affects carbon flux and transformation in water column and algal growth.Microcystis blooms significantly increase AOC levels in water,but the microbial transformation process of Microcystis-derived AOC in the presence of EOC remain poorly understood.We conducted a simulated experiment by introducing^(13)C-sodium bicarbonate and^(13)C-glucose as substrates for indoor simulation of non-axenic Microcystis aeruginosa(M.aeruginosa)populations in a sealed system.The microbial transformation processes of AOC and EOC and their effects on M.aeruginosa growth were investigated.Results demonstrated that the addition of glucose accelerated M.aeruginosa growth and significantly increased their biomass.During the experiment,as the particulate organic carbon and nitrogen content increased,the concentrations of CO_(2)and N_(2)O were gradually decreased,while the concentration of CH4 were gradually increased.Significant differences were observed in the microbial processes involved in the uptake of AOC and EOC.Bacteria involved in AOC transformation throughout the growth period were dominated by Proteobacteria,Gemmatimonadota,Actinobacteriota,Bacteroidota,Acidobacteriota,and Firmicutes.The bacteria involved in EOC transformation were dominated by Proteobacteria,Actinobacteriota,Firmicutes,Cyanobacteria,Armatimonadota,and Bacteroidota.Linear discriminant analysis Effect Size(LEfSe)analysis revealed Massilia and Akkermansia as biomarkers involved in AOC transformation,while Ligilactobacillus was associated with EOC transformation.These findings provide valuable insights into the effects of EOC on algae-bacteria interaction,and on the dynamics of carbon and nitrogen cycling among M.aeruginosa and its associated bacteria.
基金the PICTO project(RFCR-CT-2018-800711)funded by the European Research Fund for Coal and Steel(RFCS)and the Polish Ministry of Science and Higher Education(W93/FBWiS/2018).
文摘The research presented in this paper aimed to analyze the evolution of fractures in strata in relation to the progress of longwall mining.To achieve this objective,an introscopic probe equipped with a highresolution camera was developed,along with additional equipment that enables continuous recording of the internal surfaces of drainage boreholes exceeding 100 m in depth.The probe was utilized to test two methane drainage boreholes in the Z-3b longwall,which operates within the 501/3 coal seam of the Jankowice mine in Poland.Automatic image analysis methods were applied to evaluate the recorded images,based on a newly developed classificationsystem for fractures categorized by size and number.The results were compared with an analysis of changes in the methane capture rate from the drainage boreholes,which correlated with longwall mining progress.A strong correlation was observed between the number of fractures and the lithology of the strata layers.The largest number of fractures and their evolution were recorded in the coal layers,followed by the shale layers,while the sandstone layers exhibited the least number of fractures.Based on parallel measurements of the methane capture rate from the drainage boreholes during the progress of longwall mining,the extent of the strata's fracture zone was determined to range from 6 m to 36 m.Within the fracture zone,the strata are highly fractured,which leads to an increase in methane emissions through seepage and diffusion processes.
基金supported by the National Natural Science Foundation of China (Nos.22177025,21807021,22177026)the Science Fund for Creative Research Groups of the Natural Science Foundation of Hebei Province (No.B2021201038)+1 种基金the Foundation of Hebei University (No.050001-513300201004)the Director’s Foundation of the Key Laboratory of Medicinal Chemistry and Molecular Diagnosis,Ministry of Education,Hebei University (No.MCMDZR2024003)。
文摘Schizophrenia(SCZ) is a severe mental disorder with an unclear pathogenesis.Increasing evidence suggests that oxidative stress(OS) may contribute to the neuropathological processes underlying SCZ.Biothiols,key endogenous antioxidants,have been proposed as potential biomarkers for the disease.However,due to the presence of the blood-brain barrier(BBB),fluorescent probes are rarely used to image biothiols in the brain of SCZ models.In this study,a series of fluorescent probes for biothiols were developed using dicyanoisophorone derivatives as fluorophores known for their excellent optical properties,and carboxylic esters as recognition units.A parallel synthesis and rapid screening strategy was employed to construct and optimize these probes.By introducing trifluoromethyl and benzothiazole groups into the fluorophore,the emission wavelength was successfully shifted into the near-infrared region.Additionally,various trifluoromethyl-substituted aromatic and nitrogen heterocyclic compounds were incorporated to optimize the carboxylic esters,thereby improving the probes' reactivity and lipophilicity.Systematic evaluation of the physicochemical characteristics,and optical performance led to the identification of DCIBT-11 as the most promising candidate.DCI-BT-11 demonstrated excellent BBB permeability and a good response to biothiols both in vitro and in vivo.Notably,DCI-BT-11 was used for the first time to visualize biothiol flux and assess the therapeutic effects of the antioxidant N-acetylcysteine(NAC) in the brains of SCZ mouse models,offering new insights into the role of OS in the pathogenesis and treatment of SCZ.
基金supported by the National Key Research and Development Program of China (No.2024YFA1509600)National Natural Science Foundation of China (Nos.22474010 and 22274012)the Fundamental Research Funds for the Central Universities (No.2233300007)。
文摘Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of multiple RSSs significant for evaluating ferroptosis therapy.However,the traditional techniques,including fluorescent(FL) imaging and electrospray ionization-based mass spectrometry(MS) detection,cannot achieve the discrimination of different RSSs.Herein,simultaneous MS detection of multiple RSSs,including cysteine(Cys),homocysteine(Hcy),glutathione(GSH) and hydrogen sulfide(H_(2)S),was obtained upon enhancing ionization efficiency by a fluorescent probe(NBD-O-1).Based on the interaction between NBD-O-1 and RSSs,the complex of RSSs with a fragment of NBD-O-1 can be generated,which can be easily ionized for MS detection in the negative mode.Therefore,the intracellular RSSs can be well detected upon the incubation of He La cells with the probe of NBD-O-1,exhibiting the total RSS levels by the FL imaging and further providing expression of each RSS by enhanced MS detection.Furthermore,the RSSs during ferroptosis in He La cells have been evaluated using the present strategy,demonstrating the potential for ferroptosis examinations.This work has made an unconventional application of a fluorescent probe to enhance the detection of multiple RSSs by MS,providing significant molecular information for addressing the ferroptosis mechanism.
基金supported by the Starting growth Technological R&D Program(TIPS Program,RS-2024-00468327)funded by the Ministry of SMEs and Startups(MSS,Korea)in 2024 and National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(Nos.2023R1A2C300453411 and RS2024-00411069),Glocal University 30 project(Molecular Imaging Center,POSTECH).
文摘Cell-selective fluorescent probes have emerged as essential tools for live-cell imaging,enabling the differentiation of specific cell types within complex biological systems.Unlike traditional antibody-based methods that target extracellular proteins,small-molecule probes can access intracellular environments and exploit diverse biochemical features for selective retention or activation.This perspective categorizes the mechanisms of cell selectivity into five principal strategies:Protein-oriented,carbohydrate-oriented,lipid-oriented,gating-oriented,and metabolism-oriented live-cell distinctions.Each class capitalizes on a unique cellular trait ranging from protein expression and membrane composition to transporter activity and metabolic enzyme presence.We discuss representative examples of each mechanism,outline a decision-tree workflow for elucidating a new probe's mode of action,and highlight how understanding these mechanisms is critical for both basic biological research and therapeutic probe design.Looking ahead,the development of such mechanism-informed cell-specific probes holds promise for advancing precision cell targeting in biomedical applications.
基金supported by the Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A04013)the National Natural Science Foundation of China(82204610)+1 种基金the Qihang Talent Program(L2022046)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-041 and L2021029).
文摘Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.
基金supported by the National Natural Science Foundation of China (No.22007008)the LiaoNing Revitalization Talents Program (No.XLYC1907021)the Fundamental Research Funds for the Central Universities (Nos.DUT23YG120,DUT19RC(3)009)。
文摘The study of target proteins is crucial for understanding molecular interactions and developing analytical platforms,therapeutic agents and functional tools.Herein,we present a novel nanoplatform activated by near-infrared(NIR) light for triple-modal proteins study,which enabling target protein labeling,enrichment and visualization.Azido-naphthalimide-coated upconversion nanoparticles(UCNPs) serve as NIR light-responsive nanoplatforms,showing promising applications in studying interactions between various bioactive molecules and proteins in living systems.Under NIR light irradiation,azido-naphthalimides are activated by ultraviolet(UV) and blue light emitted from UCNPs and the resulting amino-naphthalimides intermediate not only crosslink nearby target proteins but also enable imaging performance.We demonstrate that this nanoplatform is capable of selective protein labeling and imaging in complex protein environments,achieving specific labeling and imaging of both intracellular and extracellular proteins in mammalian cells as well as bacteria.Furthermore,in vivo protein labeling has been achieved using this novel NIR light-activatable nanoplatform.This technique will open new avenues for discoveries and mechanistic interrogation in chemical biology.
基金the financial support from the National Natural Science Foundation of China(Nos.22377097,22307036,22074114)Natural Science Foundation of Hubei Province of China(Nos.2020CFB623,2021CFB556)Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education(No.LCX202305)。
文摘The detection of amino acid enantiomers holds significant importance in biomedical,chemical,food,and other fields.Traditional chiral recognition methods using fluorescent probes primarily rely on fluorescence intensity changes,which can compromise accuracy and repeatability.In this study,we report a novel fluorescent probe(R)-Z1 that achieves effective enantioselective recognition of chiral amino acids in water by altering emission wavelengths(>60 nm).This water-soluble probe(R)-Z1 exhibits cyan or yellow-green luminescence upon interaction with amino acid enantiomers,enabling reliable chiral detection of 14 natural amino acids.It also allows for the determination of enantiomeric excess through monitoring changes in luminescent color.Additionally,a logic operation with two inputs and three outputs was constructed based on these optical properties.Notably,amino acid enantiomers were successfully detected via dual-channel analysis at both the food and cellular levels.This study provides a new dynamic luminescence-based tool for the accurate sensing and detection of amino acid enantiomers.
基金supported by theNationalNatural Science Foundation of China(No.U23A20305)National Key Research and Development Program of China(No.2022YFB3102900)+1 种基金Innovation Scientists and Technicians Troop Construction Projects of Henan Province,China(No.254000510007)Key Research and Development Project of Henan Province(No.221111321200).
文摘To address the challenge of low survival rates and limited data collection efficiency in current virtual probe deployments,which results from anomaly detection mechanisms in location-based service(LBS)applications,this paper proposes a novel virtual probe deployment method based on user behavioral feature analysis.The core idea is to circumvent LBS anomaly detection by mimicking real-user behavior patterns.First,we design an automated data extraction algorithm that recognizes graphical user interface(GUI)elements to collect spatio-temporal behavior data.Then,by analyzing the automatically collected user data,we identify normal users’spatio-temporal patterns and extract their features such as high-activity time windows and spatial clustering characteristics.Subsequently,an antidetection scheduling strategy is developed,integrating spatial clustering optimization,load-balanced allocation,and time window control to generate probe scheduling schemes.Additionally,a self-correction mechanism based on an exponential backoff strategy is implemented to rectify anomalous behaviors andmaintain system stability.Experiments in real-world environments demonstrate that the proposed method significantly outperforms baseline methods in terms of both probe ban rate and task completion rate,while maintaining high time efficiency.This study provides a more reliable and clandestine solution for geosocial data collection and lays the foundation for building more robust virtual probe systems.
基金supported by the Natural Science Foundation of Hubei Province (Nos.2023AFB376 and 2024AFD287)National Key Research and Development Program (No.2023YFC3503804)the National Natural Science Foundation of China (No.22077044)。
文摘Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.
文摘Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particular spiritual world, merit and drawback of her character, her love value and conflicts about her thought. Analyzing into internal factors for Anna 's tragedy would offer further appreciation to readers.
基金supported by the National Natural Science Foundation of China(Nos.81872815,81573363,81690263,21372063)the Natural Science Foundation of Shanghai(No.16ZR1403500)
文摘Nanoemulsions(NEs)and nanosuspensions(NSs)show great potential in enhancing the ocular bioavailability of therapeutics through topical delivery.However,transocular fate of intact NEs and NSs is still inconclusive.In this study,an aggregation-caused quenching fluorescent probe is used to track precorneal retention and transocular transportation of intact NEs and NSs,while coumarin 6 is used to mimick the cargo.NEs show superior precorneal retention to NSs.Both the two types of nanocarriers can permeate into but not across the cornea.The smaller NEs(100 nm)permeate better into the cornea than the bigger ones(210 nm).Nanocarriers in the cornea serves as depots.The released cargo molecules can penetrate across the cornea and diffuse into the lens.Moreover,the conjunctiva-scleral route may be potential to deliver drugs to the back of the eye,In conclusion,the study provides useful tools and information in the field of transocular transportation of nanoparticles.
