Uranium exploration breakthrough was extremely rare in an aeolian depositional system.In order to know the complicate characteristics of oxidation associated closely with uranium mineralization in the aeolian depositi...Uranium exploration breakthrough was extremely rare in an aeolian depositional system.In order to know the complicate characteristics of oxidation associated closely with uranium mineralization in the aeolian depositional system,petrology and mineralogy markers of the oxidation and its genetic mechanisms are identified and illustrated by fieldwork,thin section analysis and scanning electron microscopy test,based on 2 field outcrops in Zhenyuan County in the southwest of the Tianhuan depression in the Ordos Basin and the core of 2 wells in the north and south of Ordos Basin.The results showed:the typical macroscopic indicator of primary oxidation was the red fine sediments in the aeolian interdune with a thickness of 10-50 cm,and the microscopic characteristics of primary oxidation were the minerals such as hematite,ilmenite,and the irony matrix rich in fine-grained dolomite and biotite;the phreatic oxidation was manifested as the red sandstone with limonite horizontal layer with a thickness of 1-4 cm and a width of 60 cm-1 m,and the circular limonite nodules with a diameter of 3-7 cm,in which there was intergranular limonite cement;the interlayer oxidation was characterized by lenticular tongue and tapered red sandstone with a length of 1-10 m and a width of 10 cm-5m,in which detrital particles are coated with hematite and hematite was distributed inside the rhombus dolomite.The paleoclimate of the sedimentary period,the water-table movement and the pore and permeability conditions of the sand body were the key factors for the formation of different oxidation types in the aeolian depositional system.展开更多
AIM： To observe the relationship between ethanol-induced oxidative damage in human primary cultured hepatocytes and cytochrome P450 2E1 （CYP2E1） activity, in order to address if inhibition of CYP2E1 could attenuate...AIM： To observe the relationship between ethanol-induced oxidative damage in human primary cultured hepatocytes and cytochrome P450 2E1 （CYP2E1） activity, in order to address if inhibition of CYP2E1 could attenuate ethanol- induced cellular damage. METHODS： The dose-dependent （25-100 mmol/L） and time-dependent （0-24 h） exposures of primary human cultured hepatocytes to ethanol were carried out. CYP2E1 activity and protein expression were detected by spectrophotometer and Western blot analysis respectively. Hepatotoxicity was investigated by determination of lactate dehydrogenase （LDH） and aspartate transaminase （AST） level in hepatocyte culture supernatants, as well as the intracellular formation of malondialdehyde （MDA）. RESULTS： A dose-and time-dependent response between ethanol exposure and CYP2E1 activity in human hepatocytes was demonstrated. Moreover, there was a time-dependent increase of CYP2E1 protein after 100 mmol/L ethanol exposure. Meanwhile, ethanol exposure of hepatocytes caused a time-dependent increase of cellular MDA level, LDH, and AST activities in supernatants. Furthermore, the inhibitor of CYP2E1, diallyl sulfide （DAS） could partly attenuate the increases of MDA, LDH, and AST in human hepatocytes. CONCLUSION： A positive relationship between ethanolinduced oxidative damage in human primary cultured hepatocytes and CYP2E1 activity was exhibited, and the inhibition of CYP2E1 could partly attenuate ethanol-induced oxidative damage.展开更多
The prevalence of domestic and industrial electrical appliances has raised concerns about the health risk of extremely low-frequency magnetic fields（ELF-MFs）. At present, the effects of ELF-MFs on the central nervou...The prevalence of domestic and industrial electrical appliances has raised concerns about the health risk of extremely low-frequency magnetic fields（ELF-MFs）. At present, the effects of ELF-MFs on the central nervous system are still highly controversial, and few studies have investigated its effects on cultured neurons. Here, we evaluated the biological effects of different patterns of ELF-MF exposure on primary cultured hippocampal neurons in terms of viability, apoptosis, genomic instability,and oxidative stress. The results showed that repeated exposure to 50-Hz 2-mT ELF-MF for 8 h per day after different times in culture decreased the viability and increased the production of intracellular reactive oxidative species in hippocampal neurons. The mechanism was potentially related to the up-regulation of Nox2 expression.Moreover, none of the repeated exposure patterns had significant effects on DNA damage, apoptosis, or autophagy, which suggested that ELF-MF exposure has no severe biological consequences in cultured hippocampal neurons.展开更多
基金supported by the National Key Research and Development Program of China (No.2018YFC0604202)from the Ministry of Science and Technology of China and the International Geosciences Program (No.IGCP675)
文摘Uranium exploration breakthrough was extremely rare in an aeolian depositional system.In order to know the complicate characteristics of oxidation associated closely with uranium mineralization in the aeolian depositional system,petrology and mineralogy markers of the oxidation and its genetic mechanisms are identified and illustrated by fieldwork,thin section analysis and scanning electron microscopy test,based on 2 field outcrops in Zhenyuan County in the southwest of the Tianhuan depression in the Ordos Basin and the core of 2 wells in the north and south of Ordos Basin.The results showed:the typical macroscopic indicator of primary oxidation was the red fine sediments in the aeolian interdune with a thickness of 10-50 cm,and the microscopic characteristics of primary oxidation were the minerals such as hematite,ilmenite,and the irony matrix rich in fine-grained dolomite and biotite;the phreatic oxidation was manifested as the red sandstone with limonite horizontal layer with a thickness of 1-4 cm and a width of 60 cm-1 m,and the circular limonite nodules with a diameter of 3-7 cm,in which there was intergranular limonite cement;the interlayer oxidation was characterized by lenticular tongue and tapered red sandstone with a length of 1-10 m and a width of 10 cm-5m,in which detrital particles are coated with hematite and hematite was distributed inside the rhombus dolomite.The paleoclimate of the sedimentary period,the water-table movement and the pore and permeability conditions of the sand body were the key factors for the formation of different oxidation types in the aeolian depositional system.
基金Supported by the National Science Foundation of China, No. 30271130
文摘AIM： To observe the relationship between ethanol-induced oxidative damage in human primary cultured hepatocytes and cytochrome P450 2E1 （CYP2E1） activity, in order to address if inhibition of CYP2E1 could attenuate ethanol- induced cellular damage. METHODS： The dose-dependent （25-100 mmol/L） and time-dependent （0-24 h） exposures of primary human cultured hepatocytes to ethanol were carried out. CYP2E1 activity and protein expression were detected by spectrophotometer and Western blot analysis respectively. Hepatotoxicity was investigated by determination of lactate dehydrogenase （LDH） and aspartate transaminase （AST） level in hepatocyte culture supernatants, as well as the intracellular formation of malondialdehyde （MDA）. RESULTS： A dose-and time-dependent response between ethanol exposure and CYP2E1 activity in human hepatocytes was demonstrated. Moreover, there was a time-dependent increase of CYP2E1 protein after 100 mmol/L ethanol exposure. Meanwhile, ethanol exposure of hepatocytes caused a time-dependent increase of cellular MDA level, LDH, and AST activities in supernatants. Furthermore, the inhibitor of CYP2E1, diallyl sulfide （DAS） could partly attenuate the increases of MDA, LDH, and AST in human hepatocytes. CONCLUSION： A positive relationship between ethanolinduced oxidative damage in human primary cultured hepatocytes and CYP2E1 activity was exhibited, and the inhibition of CYP2E1 could partly attenuate ethanol-induced oxidative damage.
基金supported by the National Natural Science Foundation(31170799 and 30872082)the National Basic Research Development Program(973 Program)of China(2011CB503702)
文摘The prevalence of domestic and industrial electrical appliances has raised concerns about the health risk of extremely low-frequency magnetic fields（ELF-MFs）. At present, the effects of ELF-MFs on the central nervous system are still highly controversial, and few studies have investigated its effects on cultured neurons. Here, we evaluated the biological effects of different patterns of ELF-MF exposure on primary cultured hippocampal neurons in terms of viability, apoptosis, genomic instability,and oxidative stress. The results showed that repeated exposure to 50-Hz 2-mT ELF-MF for 8 h per day after different times in culture decreased the viability and increased the production of intracellular reactive oxidative species in hippocampal neurons. The mechanism was potentially related to the up-regulation of Nox2 expression.Moreover, none of the repeated exposure patterns had significant effects on DNA damage, apoptosis, or autophagy, which suggested that ELF-MF exposure has no severe biological consequences in cultured hippocampal neurons.
