Phosphatase and tensin homolog deleted on chromosome 10(PTEN)messenger RNA(mRNA)delivery has fueled a great hope for tumor immunotherapy via augmenting the immune sensitivity in many human cancers.However,therapeutic ...Phosphatase and tensin homolog deleted on chromosome 10(PTEN)messenger RNA(mRNA)delivery has fueled a great hope for tumor immunotherapy via augmenting the immune sensitivity in many human cancers.However,therapeutic efficacy and clinical translation are limited by inadequate mRNA expression,insufficient immune stimulation and stringent storage requirements.Herein,inspired by the intrinsic properties of metal ions and exosomes,we developed a biomimetic delivery system(Mn-NP@PM)with superior stability for precise colorectal cancer immunotherapy.This platform employs adjuvant-metal-ion chelation for PTEN mRNA loading and PD-L1 antibodies(αPD-L1)-modified monocyte-macrophage membrane coating for mRNA protection and tumor targeting.Mn^(2+) was specifically selected due to its capacity for reversible mRNA binding through weak non-electrostatic interactions,facilitating efficient release,while simultaneously activating the stimulator of interferon genes(STING)pathway.Importantly,Mn-NP@PM exhibited membrane fusion for immediate cytosolic mRNA delivery,bypassing endo-lysosomal escape,optimizing transportation efficiency.Clinical-data-driven analyses further demonstrated that Mn-NP@PM-mediated PTEN restoration significantly increased T-cell infiltration and strengthened antitumor immunity in humanized patient derived xenograft(PDX)models.Collectively,this biomimetic,metal-ion-chelating,membrane-coated mRNA delivery system represents a versatile and clinically translatable strategy for personalized cancer immunotherapy.展开更多
The deuterium labeling has garnered significant interest in drug discovery due to its critical role on improving pharmacokinetic and metabolic properties.However,despite its pharmaceutical value,the general and rapid ...The deuterium labeling has garnered significant interest in drug discovery due to its critical role on improving pharmacokinetic and metabolic properties.However,despite its pharmaceutical value,the general and rapid syntheses of aromatic scaffolds that contains deuterium remain an important yet elusive task.State-of-the-art approaches mainly relied on the transition metal-catalyzed C-H deuteration via the assistance of directing groups(DGs),which often suffered from over-deuteration and lengthy step counts required for installation and/or removal of DG.Herein,we report a generalizable synthetic linchpin strategy for the facile preparation of the ortho-deuterated aromatic core.Through capture of aryne-derived 1,3-zwitterion with heavy water,we synthesized an array of ortho-deuterated aryl sulfonium salts.These novel linchpins not only participated the transition metal catalyzed cross-coupling reaction as nucleophiles,but also served as aryl radical reservoirs under photochemical or electrochemical conditions,enabling facile and precise access to structurally diverse deuterated aromatics.Moreover,we have disclosed a novel EDA complex enabled direct arylation of phosphines under visible-light irradiation,further expanding the utility of our platform approach.展开更多
Controllable photoreaction transition of photosensitizers(PSs)provides a highly promising approach for achieving efficient photodynamic therapy(PDT).However,tumor microenvironment-triggered phototransition remains a s...Controllable photoreaction transition of photosensitizers(PSs)provides a highly promising approach for achieving efficient photodynamic therapy(PDT).However,tumor microenvironment-triggered phototransition remains a significant challenge and has not yet been reported.In this work,we develop a hydrogen bond self-assembly(HBSA)strategy that is triggered by the acidic tumor microenvironment to enable the photodynamic transition of tetra(4-carboxylphenyl)porphyrin(TCPP)PSs from type Ⅱ to type Ⅰ reactions.Upon self-assembly of TCPP monomers into TCPP assemblies(TCPP-ass),the generated reactive oxygen species shift from singlet oxygen to superoxide anions,which induces caspase-3/GSDME-mediated programmed pyroptosis,enabling rapid and complete solid tumor elimination with minimized adverse effects and enhanced therapeutic efficacy.Crucially,the HBSA process occurs exclusively within tumor cells,and this tumor-specific self-assembly strategy not only utilizes high tissue penetration of TCPP molecular-PSs,but also avoids phototoxicity caused by the formation and accumulation of TCPP-ass nano-PSs in normal tissue,providing an innovative approach for precise cancer therapy.展开更多
Expanding the specific surface area of substrates and carrying out precise surface engineering of imprinted nanocavities are crucial methods for enhancing the identification efficiency of molecularly imprinted polymer...Expanding the specific surface area of substrates and carrying out precise surface engineering of imprinted nanocavities are crucial methods for enhancing the identification efficiency of molecularly imprinted polymers(MIPs).To implement this synergistic strategy,bioinspired surface engineering was used to incorporate dual covalent receptors via precise post-imprinting modifications(PIMs)onto mesoporous silica nanosheets.The prepared sorbents(denoted as‘‘D-PMIPs”)were utilized to improve the specific identification of adenosine 5-monophosphate(AMP).Significantly,the mesoporous silica nanosheets possess a high surface area of approximately 498.73 m^(2)·g^(-1),which facilitates the formation of abundant specific recognition sites in the D-PMIPs.The dual covalent receptors are valuable for estab-lishing the spatial orientation and arrangement of AMP through multiple cooperative interactions.PIMs enable precise site-specific functionalization within the imprinted cavities,leading to the tailor-made formation of complementary binding sites.The maximum number of high-affinity binding sites(Nmax)of the D-PMIPs is 39.99 lmol·g^(-1),which is significantly higher than that of imprinted sorbents with a sin-gle receptor(i.e.,S-BMIPs or S-PMIPs).The kinetic data of the D-PMIPs can be effectively described by a pseudo-second-order model,indicating that the main binding mechanism involves synergistic chemisorption from boronate affinity and the pyrimidine base.This study suggests that using dual cova-lent receptors and PIMs is a reliable approach for creating imprinted sorbents with high selectivity,allow-ing for the controlled engineering of imprinted sites.展开更多
This paper systematically elucidates the application of plasma technology in cancer treatment,including its principles,case studies,comparative advantages over traditional methods,challenges,and countermeasures.Plasma...This paper systematically elucidates the application of plasma technology in cancer treatment,including its principles,case studies,comparative advantages over traditional methods,challenges,and countermeasures.Plasma technology targets and eliminates cancer cells with precision through physical,chemical,and immune-regulatory mechanisms,offering high accuracy and low side effects.International applications include plasma scalpels in the United States,combined chemotherapy and low-temperature plasma therapy in Russia,and plasma-targeted capture technology in China.However,plasma technology faces technical hurdles and clinical application barriers,requiring interdisciplinary collaboration and industry-academia-research cooperation to advance its development.展开更多
Noble metal nanoclusters have attracted great scientific interests due to their tempting properties and applications.Significant strides have been made in recent years to synthesis atomically precise nanoclusters by u...Noble metal nanoclusters have attracted great scientific interests due to their tempting properties and applications.Significant strides have been made in recent years to synthesis atomically precise nanoclusters by utilizing polyoxometalates as protecting ligands.Remarkably,the group of Professor Suzuki from the University of Tokyo has made a great contribution in this field.Here we spotlight on four related papers in this area and present a brief highlight of these publications.展开更多
With the development of education and technology,the construction of research public platforms has emerged as a critical initiative for many universities and top-tier public hospitals.The core and most fundamental fun...With the development of education and technology,the construction of research public platforms has emerged as a critical initiative for many universities and top-tier public hospitals.The core and most fundamental function of a basic public platform is to aggregate large instruments and specific resources,providing open services for instrumental analysis and sample testing.Optimized management and high-quality,efficient services are essential for such platforms.This article elucidates the construction of a research public platform in West China Hospital,focusing on the adoption of hierarchical management and precise services.The core of the hierarchical management lies in building a multi-level service platform composed of routine support platforms,advanced technology platforms,and specially qualification platforms,while establishing a talent hierarchy that differentiates between core and routine positions.This structure is designed to accurately meet the diverse needs of users and enhance resource efficiency.By implementing user access control with differentiated permissions for internal and external users and a dynamic credit-based review system,the laboratory can ensure safe and efficient operations.The four service modes—instrument usage,in-lab experiments,sample testing,and collaborative projects—are precisely aligned with various research scenarios.Proactive engagement with grant-funded projects,customized services for research groups,and a multidimensional training system further strengthen the platform's support for major scientific research tasks.Through systematic management and service innovation,this model achieves efficient integration and sustainable development of platform resources,providing a valuable reference for the construction of public platforms in similar medical institutions.展开更多
Metal nanoclusters with well-defined atomic structures offer significant promise in the field of catalysis due to their sub-nanometer size and tunable organic-inorganic hybrid structural features.Herein,we successfull...Metal nanoclusters with well-defined atomic structures offer significant promise in the field of catalysis due to their sub-nanometer size and tunable organic-inorganic hybrid structural features.Herein,we successfully synthesized an 11-core copper(Ⅰ)-alkynyl nanocluster(Cu11),which is stabilized by alkynyl ligands derived from a photosensitive rhodamine dye molecule.Notably,this Cu11cluster exhibited excellent photocatalytic hydrogen evolution activity(8.13 mmol g-1h-1)even in the absence of a mediator and noble metal co-catalyst.Furthermore,when Cu11clusters were loaded onto the surface of TiO_(2)nanosheets,the resultant Cu11@TiO_(2)nanocomposites exhibited a significant enhancement in hydrogen evolution efficiency,which is 60 times higher than that of pure TiO_(2)nanosheets.The incorporation of Cu11clusters within the Cu11@TiO_(2)effectively inhibits the recombination of photogenerated electrons and holes,thereby accelerating the charge separation and migration in the composite material.This work introduces a novel perspective for designing highly active copper cluster-based photocatalysts.展开更多
This study explores the use of the Global Navigation Satellite System(GNSS)precise point positioning(PPP)technology to determine the natural vibration periods of towering structures through simulations and field testi...This study explores the use of the Global Navigation Satellite System(GNSS)precise point positioning(PPP)technology to determine the natural vibration periods of towering structures through simulations and field testing.During the simulation phase,a GNSS receiver captured vi-bration waveforms generated by a single-axis motion simulator based on preset signal parameters,analyzing how different satellite system configurations affect the efficiency of extracting vibration parameters.Subsequently,field tests were conducted on a high-rise steel singletube tower.The results indicate that in the simulation environment,no matter the PPP positioning data under single GPS or multisystem combination,the vibration frequency of singleaxis motion simulator can be accurately extracted after frequency do-main analysis,with multisystem setups providing more precise amplitude parameters.In the field test,the natural vibration periods of the main vibration modes of high-rise steel single-tube tower measured by PPP technology closely match the results of the first two modes derived from finite element analysis.The first mode period calculated by the em-pirical formula is approximately 6%higher than those determined through finite element analysis and PPP.This study demonstrates the potential of PPP for structural vibration analysis,offering significant benefits for assessing dynamic responses and monitoring the health of towering structures.展开更多
Strategic fluorination of solvent,a prominent strategy to enhance the electrolyte oxidation resistance and engineer a robust cathode-electrolyte interface,is crucial for realizing high-voltage lithium-ion batteries.Ac...Strategic fluorination of solvent,a prominent strategy to enhance the electrolyte oxidation resistance and engineer a robust cathode-electrolyte interface,is crucial for realizing high-voltage lithium-ion batteries.Actually,the adaptability of fluorinated solvents to high voltages is critically determined by the degree of fluorination and the fluorination site,yet lacks systematic design principles.Herein,we introduce a solvent screening descriptor based on ionization energy and Fukui function to assess molecular and site-specific reactivity.Computational and experimental results demonstrate that an optimal solvent with low ground-state energies and reactive sites is required as an ideal candidate for high-voltage electrolytes.Among derivatives from anisole,(trifluoromethoxy)benzene is identified as a superior candidate,enabling the formulation of a low reactivity solution(LPT)as electrolyte.Remarkably,the prepared Li‖LCO cell using LPT electrolyte maintained a high-capacity retention of 78.8%after 600 cycles at 4.5 V.In addition,the formation of an inorganic-rich interphase from LPT electrolyte effectively suppresses structural degradation to ensure a fast dynamic behavior.The utilization of LPT electrolyte also greatly reduces the amount of heat released and the production of O_(2)gas,which is favorable for addressing thermal runaway hazards.This screening strategy offers a practical approach for the design of flame-retardant high-voltage electrolytes.展开更多
Navigation satellites generally use satellite-ground and inter-satellite observation data for precise orbit determination.In orbit determination,the satellite position is often referenced to the satellite’s centroid,...Navigation satellites generally use satellite-ground and inter-satellite observation data for precise orbit determination.In orbit determination,the satellite position is often referenced to the satellite’s centroid,while the observational measurements are referenced to the satellite’s antenna phase center.The deviation between the satellite’s centroid and the antenna phase center forms the satellite antenna phase center error,which affects the precision of orbit determination.This paper takes a global navigation satellite system(GNSS)MEO satellite as an example and analyzes the actual situation of the satellite antenna phase center deviation and phase center variation based on the ground calibration data of the in-orbit satellite antenna phase center and the satellite’s in-orbit working status.The analysis shows that the antenna phase center variation caused by the satellite’s in-orbit operation is only at the centimeter level,which has a limited impact on orbit determination accuracy.The main source of precise orbit determination error is the satellite antenna phase center deviation,which can be corrected using ground calibration data.展开更多
Atomically precise metal nanoclusters are an emerging type of nanomaterial which has diverse interfacial metal-ligand coordination motifs that can significantly affect their physicochemical properties and functionalit...Atomically precise metal nanoclusters are an emerging type of nanomaterial which has diverse interfacial metal-ligand coordination motifs that can significantly affect their physicochemical properties and functionalities.Among that,Cu nanoclusters have been gaining continuous increasing research attentions,thanks to the low cost,diversified structures,and superior catalytic performance for various reactions.In this review,we first summarize the recent progress regarding the synthetic methods of atomically precise Cu nanoclusters and the coordination modes between Cu and several typical ligands and then discuss the catalytic applications of these Cu nanoclusters with some explicit examples to explain the atomical-level structure-performance relationship.Finally,the current challenges and future research perspectives with some critical thoughts are elaborated.We hope this review can not only provide a whole picture of the current advances regarding the synthesis and catalytic applications of atomically precise Cu nanoclusters,but also points out some future research visions in this rapidly booming field.展开更多
The vehicle-road coupling dynamics problem is a prominent issue in transportation,drawing significant attention in recent years.These dynamic equations are characterized by high-dimensionality,coupling,and time-varyin...The vehicle-road coupling dynamics problem is a prominent issue in transportation,drawing significant attention in recent years.These dynamic equations are characterized by high-dimensionality,coupling,and time-varying dynamics,making the exact solutions challenging to obtain.As a result,numerical integration methods are typically employed.However,conventional methods often suffer from low computational efficiency.To address this,this paper explores the application of the parameter freezing precise exponential integrator to vehicle-road coupling models.The model accounts for road roughness irregularities,incorporating all terms unrelated to the linear part into the algorithm's inhomogeneous vector.The general construction process of the algorithm is detailed.The validity of numerical results is verified through approximate analytical solutions(AASs),and the advantages of this method over traditional numerical integration methods are demonstrated.Multiple parameter freezing precise exponential integrator schemes are constructed based on the Runge-Kutta framework,with the fourth-order four-stage scheme identified as the optimal one.The study indicates that this method can quickly and accurately capture the dynamic system's vibration response,offering a new,efficient approach for numerical studies of high-dimensional vehicle-road coupling systems.展开更多
This article provides a comprehensive review of the advancements in the application of artificial intelligence(AI)technology in the modernization of traditional Chinese medicine(TCM)compound prescriptions,and emphasiz...This article provides a comprehensive review of the advancements in the application of artificial intelligence(AI)technology in the modernization of traditional Chinese medicine(TCM)compound prescriptions,and emphasizes recent research developments,including intelligent design,prediction of mechanisms of action,and precise application of TCM compound prescriptions.The integration of multi-omics data,deep learning algorithms,and knowledge graph technologies has established novel technical avenues for the modernization research of TCM.This study systematically analyzes the advantages and challenges associated with AI technologies in the research of TCM compound prescriptions,highlighting issues such as data heterogeneity,limited interpretability of AI models,and the absence of standardized procedures.Furthermore,this article examines the prospective developmental trajectories within this field,highlighting the importance of synergistic collaboration between AI and traditional pharmacology to improve the clinical applicability and effectiveness of TCM.The objective is to offer valuable insights into the modernization of TCM driven by AI and to stimulate further research in this area.展开更多
Atomically precise palladium(Pd)clusters are emerging as versatile nanomaterials with applications in catalysis and biomedicine.This study explores the synthesis,structure evolution,and catalytic properties of Pd clus...Atomically precise palladium(Pd)clusters are emerging as versatile nanomaterials with applications in catalysis and biomedicine.This study explores the synthesis,structure evolution,and catalytic properties of Pd clusters stabilized by cyclohexanethiol(HSC_(6)H_(11))ligands.Using electrospray ionization mass spectrometry(ESI-MS)and single-crystal X-ray diffraction(SXRD),structures of the Pd clusters ranging from Pd4(SC_(6)H_(11))8 to Pd18(SC_(6)H_(11))36 were determined.This analysis revealed a structure evolution from polygonal to elliptical geometries of the PdnS2n frameworks as the cluster size increased.UV-Vis-NIR spectroscopy,combined with quantum chemical calculations,elucidated changes in the electronic structure of the clusters.Catalytic studies on the Sonogashira cross-coupling reactions demonstrated a size-dependent decline in activity attributed to variations in structural arrangements and electronic properties.Mechanistic insights proposed a distinctive Pd(Ⅱ)-Pd(Ⅳ)catalytic cycle.This research underscores how ligands and cluster size influence the structures and properties of Pd clusters,offering valuable insights for the future design and application of Pd clusters in advanced catalysis and beyond.展开更多
Machado-Joseph disease,or spinocerebellar ataxia type 3(SCA3),represents the most common autosomal dominant cerebellar ataxia worldwide.Despite its progressive and debilitating nature,disease-modifying therapies remai...Machado-Joseph disease,or spinocerebellar ataxia type 3(SCA3),represents the most common autosomal dominant cerebellar ataxia worldwide.Despite its progressive and debilitating nature,disease-modifying therapies remain elusive.Repetitive transcranial magnetic stimulation(rTMS)has emerged as a promising non-invasive intervention;however,its clinical application has been hindered by inconsistent protocols and a lack of mechanistic understanding.A recent landmark study published in Brain Stimulation by Chen et al.addressed these challenges by combining a high-dose intermittent theta-burst stimulation(iTBS)protocol with concurrent transcranial magnetic stimulation-electroencephalography(TMS-EEG).This commentary provides an in-depth analysis of their findings,highlighting the restoration of cerebello-cortical inhibition(CBI)as a key therapeutic mechanism.Furthermore,we discuss the broader implications of this work,proposing that future translational research should integrate accelerated iTBS(aiTBS)paradigms,cortical response measurements(CRM),and individualized neuro-navigation to establish a new era of precision neuromodulation for ataxia.展开更多
Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population in...Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.展开更多
Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing can...Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing cancer detection,diagnosis,and prognostication.A narrative review of literature published from January 2015 to march 2025 was conducted using PubMed,Web of Science,and Scopus.Search terms included"gastrointestinal cancer","artificial intelligence","machine learning","deep learning","radiomics","multimodal detection"and"predictive modeling".Studies were included if they focused on clinically relevant AI applications in GI oncology.AI algorithms for GI cancer detection have achieved high performance across imaging modalities,with endoscopic DL systems reporting accuracies of 85%-97%for polyp detection and segmentation.Radiomics-based models have predicted molecular biomarkers such as programmed cell death ligand 2 expression with area under the curves up to 0.92.Large language models applied to radiology reports demonstrated diagnostic accuracy comparable to junior radiologists(78.9%vs 80.0%),though without incremental value when combined with human interpretation.Multimodal AI approaches integrating imaging,pathology,and clinical data show emerging potential for precision oncology.AI in GI oncology has reached clinically relevant accuracy levels in multiple diagnostic tasks,with multimodal approaches and predictive biomarker modeling offering new opportunities for personalized care.However,broader validation,integration into clinical workflows,and attention to ethical,legal,and social implications remain critical for widespread adoption.展开更多
With the continuous advancement of bionanomaterial technology,the design and fabrication strategies of biomimetic nanocarriers have undergone significant strategic transformations and innovations.This article systemat...With the continuous advancement of bionanomaterial technology,the design and fabrication strategies of biomimetic nanocarriers have undergone significant strategic transformations and innovations.This article systematically reviews the evolution from single-cell membrane nanovesicles to hybrid cell membrane nanovesicles integrating multiple cell membranes,culminating in cell membrane hybrid lipid nanoparticles(CM-LNPs)combining natural cell membranes or membrane proteins with engineered synthetic phospholipids.This technological progression enables the synergistic retention of multicellular biological functions and the incorporation of advantageous synthetic material properties,such as enhanced engineering flexibility and surface modifiability.Additionally,the article discusses the advantages and limitations of traditional extrusion and ultrasonication methods in the preparation of cell membrane nanovesicles,highlights the benefits and development prospects of novel microfluidic techniques in the preparation of CM-LNPs,and explores the future application prospects and challenges of CM-LNPs in the biomedical field.展开更多
基金supported by the Basic Science Center Project of the National Natural Science Foundation of China(22388101)New Cornerstone Science Foundation(NCI202318)+6 种基金the National Natural Science Foundation of China(32171398 and T242200557)the National Key R&D Program of China(2023YFA1610200 and 2022YFA1603701)Beijing Nova Program(20220484060,20230484426,and 20240484661)Beijing Natural Science Foundation(F251001)Chinese Academy of Sciences Project for Young Scientists in Basic Research(YSBR-036)the One Hundred Talents Program of Chinese Academy of Sciences(E3G551R1ZX)Chinese Academy of Medical Sciences(CAMS)and Innovation Fund for Medical Sciences(CIFMS2019-I2M-5-018).
文摘Phosphatase and tensin homolog deleted on chromosome 10(PTEN)messenger RNA(mRNA)delivery has fueled a great hope for tumor immunotherapy via augmenting the immune sensitivity in many human cancers.However,therapeutic efficacy and clinical translation are limited by inadequate mRNA expression,insufficient immune stimulation and stringent storage requirements.Herein,inspired by the intrinsic properties of metal ions and exosomes,we developed a biomimetic delivery system(Mn-NP@PM)with superior stability for precise colorectal cancer immunotherapy.This platform employs adjuvant-metal-ion chelation for PTEN mRNA loading and PD-L1 antibodies(αPD-L1)-modified monocyte-macrophage membrane coating for mRNA protection and tumor targeting.Mn^(2+) was specifically selected due to its capacity for reversible mRNA binding through weak non-electrostatic interactions,facilitating efficient release,while simultaneously activating the stimulator of interferon genes(STING)pathway.Importantly,Mn-NP@PM exhibited membrane fusion for immediate cytosolic mRNA delivery,bypassing endo-lysosomal escape,optimizing transportation efficiency.Clinical-data-driven analyses further demonstrated that Mn-NP@PM-mediated PTEN restoration significantly increased T-cell infiltration and strengthened antitumor immunity in humanized patient derived xenograft(PDX)models.Collectively,this biomimetic,metal-ion-chelating,membrane-coated mRNA delivery system represents a versatile and clinically translatable strategy for personalized cancer immunotherapy.
基金supported by the National Natural Science Foundation of China (Nos.22271010 and 21702013)。
文摘The deuterium labeling has garnered significant interest in drug discovery due to its critical role on improving pharmacokinetic and metabolic properties.However,despite its pharmaceutical value,the general and rapid syntheses of aromatic scaffolds that contains deuterium remain an important yet elusive task.State-of-the-art approaches mainly relied on the transition metal-catalyzed C-H deuteration via the assistance of directing groups(DGs),which often suffered from over-deuteration and lengthy step counts required for installation and/or removal of DG.Herein,we report a generalizable synthetic linchpin strategy for the facile preparation of the ortho-deuterated aromatic core.Through capture of aryne-derived 1,3-zwitterion with heavy water,we synthesized an array of ortho-deuterated aryl sulfonium salts.These novel linchpins not only participated the transition metal catalyzed cross-coupling reaction as nucleophiles,but also served as aryl radical reservoirs under photochemical or electrochemical conditions,enabling facile and precise access to structurally diverse deuterated aromatics.Moreover,we have disclosed a novel EDA complex enabled direct arylation of phosphines under visible-light irradiation,further expanding the utility of our platform approach.
基金supported by the National Natural Science Foundation of China(22176058)the Science and Technology Commission of Shanghai Municipality(24DX1400200,23ZR1416100,25ZR1401082)+1 种基金the Program of Introducing Talents of Discipline to Universities(B16017)the Fundamental Research Funds for the Central Universities(222201717003)。
文摘Controllable photoreaction transition of photosensitizers(PSs)provides a highly promising approach for achieving efficient photodynamic therapy(PDT).However,tumor microenvironment-triggered phototransition remains a significant challenge and has not yet been reported.In this work,we develop a hydrogen bond self-assembly(HBSA)strategy that is triggered by the acidic tumor microenvironment to enable the photodynamic transition of tetra(4-carboxylphenyl)porphyrin(TCPP)PSs from type Ⅱ to type Ⅰ reactions.Upon self-assembly of TCPP monomers into TCPP assemblies(TCPP-ass),the generated reactive oxygen species shift from singlet oxygen to superoxide anions,which induces caspase-3/GSDME-mediated programmed pyroptosis,enabling rapid and complete solid tumor elimination with minimized adverse effects and enhanced therapeutic efficacy.Crucially,the HBSA process occurs exclusively within tumor cells,and this tumor-specific self-assembly strategy not only utilizes high tissue penetration of TCPP molecular-PSs,but also avoids phototoxicity caused by the formation and accumulation of TCPP-ass nano-PSs in normal tissue,providing an innovative approach for precise cancer therapy.
基金supported by the National Natural Science Foundation of China(22078132,22108103,and U22A20413)the Open Funding Project of the National Key Labora-tory of Biochemical Engineering(2021KF-02)+3 种基金China Postdoctoral Science Foundation(2021M691301)Jiangsu Key Research and Development Program(BE2022356)the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(CPSF)(GZ20230989)Jiangsu Agricultural Independent Innovation Fund Project(CX(21)3079).
文摘Expanding the specific surface area of substrates and carrying out precise surface engineering of imprinted nanocavities are crucial methods for enhancing the identification efficiency of molecularly imprinted polymers(MIPs).To implement this synergistic strategy,bioinspired surface engineering was used to incorporate dual covalent receptors via precise post-imprinting modifications(PIMs)onto mesoporous silica nanosheets.The prepared sorbents(denoted as‘‘D-PMIPs”)were utilized to improve the specific identification of adenosine 5-monophosphate(AMP).Significantly,the mesoporous silica nanosheets possess a high surface area of approximately 498.73 m^(2)·g^(-1),which facilitates the formation of abundant specific recognition sites in the D-PMIPs.The dual covalent receptors are valuable for estab-lishing the spatial orientation and arrangement of AMP through multiple cooperative interactions.PIMs enable precise site-specific functionalization within the imprinted cavities,leading to the tailor-made formation of complementary binding sites.The maximum number of high-affinity binding sites(Nmax)of the D-PMIPs is 39.99 lmol·g^(-1),which is significantly higher than that of imprinted sorbents with a sin-gle receptor(i.e.,S-BMIPs or S-PMIPs).The kinetic data of the D-PMIPs can be effectively described by a pseudo-second-order model,indicating that the main binding mechanism involves synergistic chemisorption from boronate affinity and the pyrimidine base.This study suggests that using dual cova-lent receptors and PIMs is a reliable approach for creating imprinted sorbents with high selectivity,allow-ing for the controlled engineering of imprinted sites.
文摘This paper systematically elucidates the application of plasma technology in cancer treatment,including its principles,case studies,comparative advantages over traditional methods,challenges,and countermeasures.Plasma technology targets and eliminates cancer cells with precision through physical,chemical,and immune-regulatory mechanisms,offering high accuracy and low side effects.International applications include plasma scalpels in the United States,combined chemotherapy and low-temperature plasma therapy in Russia,and plasma-targeted capture technology in China.However,plasma technology faces technical hurdles and clinical application barriers,requiring interdisciplinary collaboration and industry-academia-research cooperation to advance its development.
基金financially supported by the Foundation of Jilin Educational Committee(JJKH20231298KJ)Natural Scientific Foundation of Jilin Province Science and Technology Department(20230101032JC)
文摘Noble metal nanoclusters have attracted great scientific interests due to their tempting properties and applications.Significant strides have been made in recent years to synthesis atomically precise nanoclusters by utilizing polyoxometalates as protecting ligands.Remarkably,the group of Professor Suzuki from the University of Tokyo has made a great contribution in this field.Here we spotlight on four related papers in this area and present a brief highlight of these publications.
基金supported by Sichuan University AI-Driven In-novative Experimental Technology Research Project(SCU2025047).
文摘With the development of education and technology,the construction of research public platforms has emerged as a critical initiative for many universities and top-tier public hospitals.The core and most fundamental function of a basic public platform is to aggregate large instruments and specific resources,providing open services for instrumental analysis and sample testing.Optimized management and high-quality,efficient services are essential for such platforms.This article elucidates the construction of a research public platform in West China Hospital,focusing on the adoption of hierarchical management and precise services.The core of the hierarchical management lies in building a multi-level service platform composed of routine support platforms,advanced technology platforms,and specially qualification platforms,while establishing a talent hierarchy that differentiates between core and routine positions.This structure is designed to accurately meet the diverse needs of users and enhance resource efficiency.By implementing user access control with differentiated permissions for internal and external users and a dynamic credit-based review system,the laboratory can ensure safe and efficient operations.The four service modes—instrument usage,in-lab experiments,sample testing,and collaborative projects—are precisely aligned with various research scenarios.Proactive engagement with grant-funded projects,customized services for research groups,and a multidimensional training system further strengthen the platform's support for major scientific research tasks.Through systematic management and service innovation,this model achieves efficient integration and sustainable development of platform resources,providing a valuable reference for the construction of public platforms in similar medical institutions.
基金supported by the National Natural Science Foundation of China(Nos.22371263 and U2004193)Natural Science Foundation of Henan Province(No.232300421225)。
文摘Metal nanoclusters with well-defined atomic structures offer significant promise in the field of catalysis due to their sub-nanometer size and tunable organic-inorganic hybrid structural features.Herein,we successfully synthesized an 11-core copper(Ⅰ)-alkynyl nanocluster(Cu11),which is stabilized by alkynyl ligands derived from a photosensitive rhodamine dye molecule.Notably,this Cu11cluster exhibited excellent photocatalytic hydrogen evolution activity(8.13 mmol g-1h-1)even in the absence of a mediator and noble metal co-catalyst.Furthermore,when Cu11clusters were loaded onto the surface of TiO_(2)nanosheets,the resultant Cu11@TiO_(2)nanocomposites exhibited a significant enhancement in hydrogen evolution efficiency,which is 60 times higher than that of pure TiO_(2)nanosheets.The incorporation of Cu11clusters within the Cu11@TiO_(2)effectively inhibits the recombination of photogenerated electrons and holes,thereby accelerating the charge separation and migration in the composite material.This work introduces a novel perspective for designing highly active copper cluster-based photocatalysts.
基金The National Natural Science Foundation of China(No.41974214).
文摘This study explores the use of the Global Navigation Satellite System(GNSS)precise point positioning(PPP)technology to determine the natural vibration periods of towering structures through simulations and field testing.During the simulation phase,a GNSS receiver captured vi-bration waveforms generated by a single-axis motion simulator based on preset signal parameters,analyzing how different satellite system configurations affect the efficiency of extracting vibration parameters.Subsequently,field tests were conducted on a high-rise steel singletube tower.The results indicate that in the simulation environment,no matter the PPP positioning data under single GPS or multisystem combination,the vibration frequency of singleaxis motion simulator can be accurately extracted after frequency do-main analysis,with multisystem setups providing more precise amplitude parameters.In the field test,the natural vibration periods of the main vibration modes of high-rise steel single-tube tower measured by PPP technology closely match the results of the first two modes derived from finite element analysis.The first mode period calculated by the em-pirical formula is approximately 6%higher than those determined through finite element analysis and PPP.This study demonstrates the potential of PPP for structural vibration analysis,offering significant benefits for assessing dynamic responses and monitoring the health of towering structures.
基金financial support from the National Natural Science Foundation of China(22522814,22278378,and 52402318)Zhejiang Provincial Natural Science Foundation of China(LDQ24E030001 and LQN25E020003)Science Foundation of Zhejiang Sci-Tech University(22212011-Y and 24212149-Y).
文摘Strategic fluorination of solvent,a prominent strategy to enhance the electrolyte oxidation resistance and engineer a robust cathode-electrolyte interface,is crucial for realizing high-voltage lithium-ion batteries.Actually,the adaptability of fluorinated solvents to high voltages is critically determined by the degree of fluorination and the fluorination site,yet lacks systematic design principles.Herein,we introduce a solvent screening descriptor based on ionization energy and Fukui function to assess molecular and site-specific reactivity.Computational and experimental results demonstrate that an optimal solvent with low ground-state energies and reactive sites is required as an ideal candidate for high-voltage electrolytes.Among derivatives from anisole,(trifluoromethoxy)benzene is identified as a superior candidate,enabling the formulation of a low reactivity solution(LPT)as electrolyte.Remarkably,the prepared Li‖LCO cell using LPT electrolyte maintained a high-capacity retention of 78.8%after 600 cycles at 4.5 V.In addition,the formation of an inorganic-rich interphase from LPT electrolyte effectively suppresses structural degradation to ensure a fast dynamic behavior.The utilization of LPT electrolyte also greatly reduces the amount of heat released and the production of O_(2)gas,which is favorable for addressing thermal runaway hazards.This screening strategy offers a practical approach for the design of flame-retardant high-voltage electrolytes.
文摘Navigation satellites generally use satellite-ground and inter-satellite observation data for precise orbit determination.In orbit determination,the satellite position is often referenced to the satellite’s centroid,while the observational measurements are referenced to the satellite’s antenna phase center.The deviation between the satellite’s centroid and the antenna phase center forms the satellite antenna phase center error,which affects the precision of orbit determination.This paper takes a global navigation satellite system(GNSS)MEO satellite as an example and analyzes the actual situation of the satellite antenna phase center deviation and phase center variation based on the ground calibration data of the in-orbit satellite antenna phase center and the satellite’s in-orbit working status.The analysis shows that the antenna phase center variation caused by the satellite’s in-orbit operation is only at the centimeter level,which has a limited impact on orbit determination accuracy.The main source of precise orbit determination error is the satellite antenna phase center deviation,which can be corrected using ground calibration data.
基金supported by the open funds of Key Laboratory of Functional Inorganic Material Chemistry (Heilongjiang University), Ministry of Education, Chinathe funding from Guangdong Natural Science Funds (No. 2023A0505050107)。
文摘Atomically precise metal nanoclusters are an emerging type of nanomaterial which has diverse interfacial metal-ligand coordination motifs that can significantly affect their physicochemical properties and functionalities.Among that,Cu nanoclusters have been gaining continuous increasing research attentions,thanks to the low cost,diversified structures,and superior catalytic performance for various reactions.In this review,we first summarize the recent progress regarding the synthetic methods of atomically precise Cu nanoclusters and the coordination modes between Cu and several typical ligands and then discuss the catalytic applications of these Cu nanoclusters with some explicit examples to explain the atomical-level structure-performance relationship.Finally,the current challenges and future research perspectives with some critical thoughts are elaborated.We hope this review can not only provide a whole picture of the current advances regarding the synthesis and catalytic applications of atomically precise Cu nanoclusters,but also points out some future research visions in this rapidly booming field.
基金Supported by the National Natural Science Foundation of China(No.U22A20246)the Key Project of Natural Science Foundation of Hebei Province of China(Basic Research Base Project)(No.A2023210064)the Science and Technology Program of Hebei Province of China(Nos.246Z1904G and 225676162GH)。
文摘The vehicle-road coupling dynamics problem is a prominent issue in transportation,drawing significant attention in recent years.These dynamic equations are characterized by high-dimensionality,coupling,and time-varying dynamics,making the exact solutions challenging to obtain.As a result,numerical integration methods are typically employed.However,conventional methods often suffer from low computational efficiency.To address this,this paper explores the application of the parameter freezing precise exponential integrator to vehicle-road coupling models.The model accounts for road roughness irregularities,incorporating all terms unrelated to the linear part into the algorithm's inhomogeneous vector.The general construction process of the algorithm is detailed.The validity of numerical results is verified through approximate analytical solutions(AASs),and the advantages of this method over traditional numerical integration methods are demonstrated.Multiple parameter freezing precise exponential integrator schemes are constructed based on the Runge-Kutta framework,with the fourth-order four-stage scheme identified as the optimal one.The study indicates that this method can quickly and accurately capture the dynamic system's vibration response,offering a new,efficient approach for numerical studies of high-dimensional vehicle-road coupling systems.
文摘This article provides a comprehensive review of the advancements in the application of artificial intelligence(AI)technology in the modernization of traditional Chinese medicine(TCM)compound prescriptions,and emphasizes recent research developments,including intelligent design,prediction of mechanisms of action,and precise application of TCM compound prescriptions.The integration of multi-omics data,deep learning algorithms,and knowledge graph technologies has established novel technical avenues for the modernization research of TCM.This study systematically analyzes the advantages and challenges associated with AI technologies in the research of TCM compound prescriptions,highlighting issues such as data heterogeneity,limited interpretability of AI models,and the absence of standardized procedures.Furthermore,this article examines the prospective developmental trajectories within this field,highlighting the importance of synergistic collaboration between AI and traditional pharmacology to improve the clinical applicability and effectiveness of TCM.The objective is to offer valuable insights into the modernization of TCM driven by AI and to stimulate further research in this area.
基金supported by the Start-Up Research Funding of Fujian Normal University(No.Y0720326K13)the National Natural Science Foundation of China(Nos.22103035 and 22033005)+2 种基金the National Key R&D Program of China(No.2022YFA1503900)Shenzhen Science and Technology Program(No.RCYX20231211090357078)Guangdong Provincial Key Laboratory of Catalysis(No.2020B121201002).
文摘Atomically precise palladium(Pd)clusters are emerging as versatile nanomaterials with applications in catalysis and biomedicine.This study explores the synthesis,structure evolution,and catalytic properties of Pd clusters stabilized by cyclohexanethiol(HSC_(6)H_(11))ligands.Using electrospray ionization mass spectrometry(ESI-MS)and single-crystal X-ray diffraction(SXRD),structures of the Pd clusters ranging from Pd4(SC_(6)H_(11))8 to Pd18(SC_(6)H_(11))36 were determined.This analysis revealed a structure evolution from polygonal to elliptical geometries of the PdnS2n frameworks as the cluster size increased.UV-Vis-NIR spectroscopy,combined with quantum chemical calculations,elucidated changes in the electronic structure of the clusters.Catalytic studies on the Sonogashira cross-coupling reactions demonstrated a size-dependent decline in activity attributed to variations in structural arrangements and electronic properties.Mechanistic insights proposed a distinctive Pd(Ⅱ)-Pd(Ⅳ)catalytic cycle.This research underscores how ligands and cluster size influence the structures and properties of Pd clusters,offering valuable insights for the future design and application of Pd clusters in advanced catalysis and beyond.
基金supported by grants from the Open Research Fund of the Zhejiang Key Laboratory of Precision Psychiatry(2025A2)the Natural Science Foundation of Zhejiang Province(LY23C090002)。
文摘Machado-Joseph disease,or spinocerebellar ataxia type 3(SCA3),represents the most common autosomal dominant cerebellar ataxia worldwide.Despite its progressive and debilitating nature,disease-modifying therapies remain elusive.Repetitive transcranial magnetic stimulation(rTMS)has emerged as a promising non-invasive intervention;however,its clinical application has been hindered by inconsistent protocols and a lack of mechanistic understanding.A recent landmark study published in Brain Stimulation by Chen et al.addressed these challenges by combining a high-dose intermittent theta-burst stimulation(iTBS)protocol with concurrent transcranial magnetic stimulation-electroencephalography(TMS-EEG).This commentary provides an in-depth analysis of their findings,highlighting the restoration of cerebello-cortical inhibition(CBI)as a key therapeutic mechanism.Furthermore,we discuss the broader implications of this work,proposing that future translational research should integrate accelerated iTBS(aiTBS)paradigms,cortical response measurements(CRM),and individualized neuro-navigation to establish a new era of precision neuromodulation for ataxia.
基金funded by the National Key R&D Project‘Innovation and Integration of Key Technologies for Integration of Agricultural Machinery and Agronomy in Weak Links of Hybrid Mid-season Rice in Hilly Areas of Southwest China’(2023YFD2301901).
文摘Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.
文摘Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing cancer detection,diagnosis,and prognostication.A narrative review of literature published from January 2015 to march 2025 was conducted using PubMed,Web of Science,and Scopus.Search terms included"gastrointestinal cancer","artificial intelligence","machine learning","deep learning","radiomics","multimodal detection"and"predictive modeling".Studies were included if they focused on clinically relevant AI applications in GI oncology.AI algorithms for GI cancer detection have achieved high performance across imaging modalities,with endoscopic DL systems reporting accuracies of 85%-97%for polyp detection and segmentation.Radiomics-based models have predicted molecular biomarkers such as programmed cell death ligand 2 expression with area under the curves up to 0.92.Large language models applied to radiology reports demonstrated diagnostic accuracy comparable to junior radiologists(78.9%vs 80.0%),though without incremental value when combined with human interpretation.Multimodal AI approaches integrating imaging,pathology,and clinical data show emerging potential for precision oncology.AI in GI oncology has reached clinically relevant accuracy levels in multiple diagnostic tasks,with multimodal approaches and predictive biomarker modeling offering new opportunities for personalized care.However,broader validation,integration into clinical workflows,and attention to ethical,legal,and social implications remain critical for widespread adoption.
基金financially supported by the National Natural Science Foundation of China(32371475)。
文摘With the continuous advancement of bionanomaterial technology,the design and fabrication strategies of biomimetic nanocarriers have undergone significant strategic transformations and innovations.This article systematically reviews the evolution from single-cell membrane nanovesicles to hybrid cell membrane nanovesicles integrating multiple cell membranes,culminating in cell membrane hybrid lipid nanoparticles(CM-LNPs)combining natural cell membranes or membrane proteins with engineered synthetic phospholipids.This technological progression enables the synergistic retention of multicellular biological functions and the incorporation of advantageous synthetic material properties,such as enhanced engineering flexibility and surface modifiability.Additionally,the article discusses the advantages and limitations of traditional extrusion and ultrasonication methods in the preparation of cell membrane nanovesicles,highlights the benefits and development prospects of novel microfluidic techniques in the preparation of CM-LNPs,and explores the future application prospects and challenges of CM-LNPs in the biomedical field.
