Purpose: To assess the efficacy and safety of Olaparib, a PARP inhibitor on progression-free survival (PFS), objective response rate (ORR) and overall survival (OS) in patients with breast and ovarian cancer. Methods:...Purpose: To assess the efficacy and safety of Olaparib, a PARP inhibitor on progression-free survival (PFS), objective response rate (ORR) and overall survival (OS) in patients with breast and ovarian cancer. Methods: Research data from clinical trials through PubMed, Science Citation Index, Elsevier Science Direct and Cochrane Library of all published studies exploring the PFS, ORR or OS of Olaparib for maintenance monotherapy on survival in breast and ovarian cancer were analysed. Pooled estimates of the ORR, weighted medians of PFS and OS from all Olaparib were calculated. Assessment of quality and level of evidence was assigned by Cochrane guidelines and guidelines of Oxford Centre for Evidence-Based Medicine. Results: Data of 893 patients (731 olaparib;162 control) from 6 trials, 2 randomised controlled trials and 4 non-randomised trials, were included. The overall median weighted PFS and OS in patients treated with Olaparib were 5.9 and 19.1 months, respectively. The pooled ORR was 25%. Olaparib showed a greater effect on PFS in patients with both wild-type BRCA and BRCA mutant gene. The most common toxicity were nausea and vomiting. Conclusions: Olaparib as maintenance monotherapy for breast and ovarian cancer is associated with promising outcomes including increased response rate and improved PFS. Its potential in clinical application is needed for further investigation in phase III trials.展开更多
In studies reporting rugby league injuries,match injuries varied depending upon participation level.To review and update pooled data estimates for rugby league injury epidemiology and add information for participation...In studies reporting rugby league injuries,match injuries varied depending upon participation level.To review and update pooled data estimates for rugby league injury epidemiology and add information for participation levels in match and training environments.A systematic review and pooled analysis for published studies reporting rugby league match and training injuries.Searches were performed in the PubMed,CINHAL,ScienceDirect,Scopus,SPORTDiscus,SpringerLink,and Wiley Online databases.Studies were considered if they reported on rugby league match or training injuries between Jan 1990 to June 2021.Two authors(DK,TC)extracted the study characteristics,numerical data and assessed the article quality,by adhering to the protocol for systematic review of observational studies(MOOSE)and the STrengthening and Reporting of OBservational studies in Epidemiology(STROBE)statement.The 46 studies included a combined exposure of 419,037 h and 18,783 injuries incorporating 158,003 match-hr and 15,706 match injuries(99.4[95%CI:97.9–101.0]per 1000 match-hr)and 264,033 training-hr and 3077 training injuries(11.8[95%CI:11.4–12.2]per 1000 training-hr).Of included studies,47.9%utilised a medical attention/treatment injury definition.There was a five-fold difference in injuries for the semi-professional participation level(431.6 per 1000 match-hr)compared with professional(RR:4.92;p<0.001)and elite(RR:3.77;p<0.001)participation levels.The hooker recorded the highest pooled injury incidence(93.1 per 1000 match-hr).Compared to the 2014 analysis there was a 10-fold increase for headneck region(RR:10.7;p<0.001)injury incidence,and more injuries for the ball carrier(RR:1.1;p?0.008)and tackler(RR:1.2;p?0.001).There was a three-fold decrease in injury incidence in the first half(RR:2.9;p<0.001)and a two-fold decrease in the second half(RR:2.3;p<0.001)of matches.While rugby league match and training injury incidence had decreased since 2014,the increase in head injuries,and greater injury rate at the semi-professional level,mean further injury prevention interventions are needed.展开更多
The organic carbon contents,carbon density and carbon storage of the soil in the Pinus koraiensis plantation ecosystem were investigated in Maoershan experimental forest farm,Shangzhi County,Heilongjiang,on the west s...The organic carbon contents,carbon density and carbon storage of the soil in the Pinus koraiensis plantation ecosystem were investigated in Maoershan experimental forest farm,Shangzhi County,Heilongjiang,on the west slope of the Zhangguangcai Mountains in northeastern China for providing data to evaluation of the carbon balance in forest ecosystem of northeastern China.These soil carbon indicators were measured in three forest types,pure P.koraiensis plantation,P.koraiensis and Betula platyphylla mixed forest,and the P.koraiensis and Quercus mongolica mixed forest.The soil carbon pool consisted of four compartments,namely L layer,F layer,H layer and B layer.With variance analysis,we found that both organic carbon content and carbon density of the soil were significantly affected by forest types,soil compartments and slope positions.The highest soil carbon density（278.63 Mg·ha^-1）.was observed in the mixed forest of P.koraiensis and Q.mongolica.The B layer had the highest carbon density（212.28 Mg·ha^-1） among all the soil compartments.In terms of slope position,the highest soil carbon density（394.18 Mg·ha^-1） presented in the low slope.Besides,soil carbon content and carbon density had a marked change with the organic matter content and vertical depth of the soil in each compartment.The results of this study implied that in the temperate humid region,the mixed ecosystem of regional Pinus koraiensis plantations and natural forest had relatively high carbon storage capability.展开更多
[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important fac...[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.展开更多
Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community asse...Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community assembly in virgin and aging MP plastispheres across different habitats is poorly understood.This study aims to assess the variations in bacterial community assembly across different niches and habitats with an in situ ex-periment,in which constructed forest wetland(FW),natural lake wetland(LW),and lotus pond wetland(LP)were habitats,and plastispheres of virgin and aging low-density polyethylene(LDPE)MPs,as well as surround-ing wetland soils were niches.Significant niche-related differences in bacterial communities were observed,with lower diversity and enrichment of potential plastic-degrading bacteria in the plastisphere than in the soil bacterial communities.Furthermore,habitat-related differences exerted a more pronounced influence on the beta-diversity patterns of the bacterial communities.The linear regression analyses indicated that the local species pool con-tributed more to bacterial community assembly in the LW wetland,whereas the relative abundance of species was the primary factor in the LP wetland.The null model analysis indicated that plastisphere bacterial communi-ties were predominantly driven by the stochastic process,with a more deterministic assembly observed in the LP wetland and soil bacterial communities.Additionally,the primary ecological process shaping plastisphere com-munities shifted from drift in the virgin LDPE to homogenising dispersal in the aging LDPE.This study provides new insights into the fate and ecological impacts of MPs in wetlands,thereby facilitating the effective regulations of plastic pollution.展开更多
The adult subventricular zone of the lateral ventricles and the subgranular zone in the hippocampal dentate gyrus(DG)are the two brain regions where neurogenesis occurs throughout life in the adult mammalian brain(Min...The adult subventricular zone of the lateral ventricles and the subgranular zone in the hippocampal dentate gyrus(DG)are the two brain regions where neurogenesis occurs throughout life in the adult mammalian brain(Ming and Song,2011).Adult quiescent hippocampal neural stem cells(NSCs)are bona fide stem cells and,when activated,give rise to newborn granule neurons in the adult brain,which play vital roles in learning,memory,mood,and affective cognition(Bonaguidi et al.,2011;Ming and Song,2011).展开更多
Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated th...Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.展开更多
Based on the theory of formation dynamics of oil/gas pools, the Dongying sag can be divided into three dynamic systems regarding the accumulation of oil and gas： the superpressure closed system, the semi-closed syste...Based on the theory of formation dynamics of oil/gas pools, the Dongying sag can be divided into three dynamic systems regarding the accumulation of oil and gas： the superpressure closed system, the semi-closed system and the normal pressure open system. Based on the analysis of genesis of superpressure in the superpressure closed system and the rule of hydrocarbon expulsion, it is found that hydrocarbon generation is related to superpressure, which is the main driving factor of hydrocarbon migration. Micro fractures formed by superpressure are the main channels for hydrocarbon migration. There are three dynamic patterns for hydrocarbon expulsion： free water drainage, hydrocarbon accumulation and drainage through micro fissures. In the superpressure closed system, the oil-driving-water process and oil/gas accumulation were completed in lithologic traps by way of such two dynamic patterns as episodic evolution of superpressure systems and episodic pressure release of faults. The oil-bearing capacity of lithologic traps is intimately related to reservoir-forming dynamic force. Quantitative evaluation of dynamic conditions for pool formation can effectively predict the oil-bearing capability of traps.展开更多
Atmospheric nitrogen (N) deposition has been poorly documented in northern China, an intensive agricultural and industrial region with large emissions of NHx and NOy. To quantify N deposition, total airborne N deposit...Atmospheric nitrogen (N) deposition has been poorly documented in northern China, an intensive agricultural and industrial region with large emissions of NHx and NOy. To quantify N deposition, total airborne N deposition was determined at three agricultural sites using a manual integrated total nitrogen input (ITNI) system during growth of winter wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) from September 2005 to May 2006. Total estimated N deposition averaged 54.9 and 43.2 kg N/hm2 across the three sites when wheat was grown to flowering and maturing, respectively. The average value was 50.2 kg N/hm2 when ryegrass was the indicator plant. Both indicator species gave similar total airborne N input results. The intermediate level of N supplied resulted in the highest N deposition, and the ratio of N acquired from deposition to total N content of the whole system decreased with increasing N supply to the roots. The contribution of atmospheric N to the total N content of the wheat and ryegrass sand culture systems ranged from 10% to 24%.展开更多
A precipitation system developed continuously along the western coastline of the Korean Peninsula and created considerable precipitation both along the coast and inland on 26 July 2011. In this study, the causes for t...A precipitation system developed continuously along the western coastline of the Korean Peninsula and created considerable precipitation both along the coast and inland on 26 July 2011. In this study, the causes for this nearshore convective system are investigated from observations and the results of model experiments. Three-dimensional radar fields clearly show that a change of wind at the surface border played an important role in the development of the nearshore convection system. The simulation results, which are very similar to the observations, show that the surface border generated and maintained the convergence zone. The roughness change enhanced the convergence, and the interaction between the deepening cold pool and downward flow maintained the convergence zone. The surface mechanical discontinuity affected by the roughness change between sea and land formed the convergence (gradient of wind stress), which induced momentum transfer to the upper layer. The cold pool created a steep gradient of potential temperature and provided the reason for the propagated convergence zone with the downward flow. The maximum value of the surface change factor, which comprises the influencing factors for the long-lasting convective system, reflects the enhancement of the system at the coast.展开更多
Building a more resilient response system to climate change for sustainable development and reducing uncertainty in China’s food markets,requires access to historical research gaps and mapping future research progres...Building a more resilient response system to climate change for sustainable development and reducing uncertainty in China’s food markets,requires access to historical research gaps and mapping future research progress for decision making.However,the lack of quantitative and objective analyses to ensure the stability and development of agroecosystems increases the complexity of agro-climatic mechanisms,which leads to uncertainty and undesirable consequences.In this paper,we review the characteristics of climate change in China(1951–2020),reveal the mechanisms of agroecosystem structure in response to climate,and identify challenges and opportunities for future efforts in the context of research progress.The aim is to improve the scientific validity and relevance of future research by clarifying agro-climatic response mechanisms.The results show that surface temperature,precipitation,and frequency of extreme weather events have increased to varying degrees in major agricultural regions of China in 1951–2020.And they have strong geographic variation,which has resulted in droughts in the north and floods in the south.Moreover,climate change has complicated the mechanisms of soil moisture,Net Primary Productivity(NPP),soil carbon pool,and crop pest structure in agroecosystems.This lends to a reduction in soil water holding capacity,NPP,soil carbon content,and the number of natural enemies of diseases and insects,which in turn affects crop yields.However,human interventions can mitigate the deterioration of these factors.We have also realized that the methodology and theory of historical research poses a great challenge to future agroecosystem.Historical and projected climate trends identified current gaps in interdisciplinary integration and multidisciplinary research required to manage diverse spatio-temporal climate change impacts on agroecosystems.Future efforts should highlight integrated management and decision making,multidisciplinary big data coupling,and numerical simulations to ensure sustainable agricultural development,ecological security,and food security in China.展开更多
An extreme rainfall event occurred over Hangzhou,China,during the afternoon hours on 24 June 2013.This event occurred under suitable synoptic conditions and the maximum 4-h cumulative rainfall amount was over 150 mm.T...An extreme rainfall event occurred over Hangzhou,China,during the afternoon hours on 24 June 2013.This event occurred under suitable synoptic conditions and the maximum 4-h cumulative rainfall amount was over 150 mm.This rainfall event had two major rainbands.One was caused by a quasi-stationary convective line,and the other by a backbuilding convective line related to the interaction of the outflow boundary from the first rainband and an existing low-level mesoscale convergence line associated with a mei-yu frontal system.The rainfall event lasted 4 h,while the back-building process occurred in 2 h when the extreme rainfall center formed.So far,few studies have examined the back-building processes in the mei-yu season that are caused by the interaction of a mesoscale convergence line and a convective cold pool.The two rainbands are successfully reproduced by the Weather Research and Forecasting(WRF)model with fourlevel,two-way interactive nesting.In the model,new cells repeatedly occur at the west side of older cells,and the backbuilding process occurs in an environment with large CAPE,a low LFC,and plenty of water vapor.Outflows from older cells enhance the low-level convergence that forces new cells.High precipitation efficiency of the back-building training cells leads to accumulated precipitation of over 150 mm.Sensitivity experiments without evaporation of rainwater show that the convective cold pool plays an important role in the organization of the back-building process in the current extreme precipitation case.展开更多
The continuous increase of human mobility combined with a relevant use of private vehicles contributes to increase the ill effects of vehicle externalities on the environment, e.g. high levels of air pollution, toxic ...The continuous increase of human mobility combined with a relevant use of private vehicles contributes to increase the ill effects of vehicle externalities on the environment, e.g. high levels of air pollution, toxic emissions, noise pollution, and on the quality of life, e.g. parking problem, traffic congestion, and increase in the number of crashes and accidents. Transport demand management plays a very critical role in achieving greenhouse gas emission reduction targets. This study demonstrates that car pooling (CP) is an effective strategy to reduce transport volumes, transportation costs and related hill externalities in agreement with EU programs of emissions reduction targets. This paper presents an original approach to solve the CP problem. It is based on hierarchical clustering models, which have been adopted by an original decision support system (DSS). The DSS helps mobility managers to generate the pools and to design feasible paths for shared vehicles. A significant case studies and obtained results by the application of the proposed models are illustrated. They demonstrate the effectiveness of the approach and the supporting decisions tool.展开更多
Effective smart healthcare frameworks contain novel and emerging solutions for remote disease diagnostics,which aid in the prevention of several diseases including heart-related abnormalities.In this context,regular m...Effective smart healthcare frameworks contain novel and emerging solutions for remote disease diagnostics,which aid in the prevention of several diseases including heart-related abnormalities.In this context,regular monitoring of cardiac patients through smart healthcare systems based on Electrocardiogram(ECG)signals has the potential to save many lives.In existing studies,several heart disease diagnostic systems are proposed by employing different state-of-the-art methods,however,improving such methods is always an intriguing area of research.Hence,in this research,a smart healthcare system is proposed for the diagnosis of heart disease using ECG signals.The proposed framework extracts both linear and time-series information on the ECG signals and fuses them into a single framework concurrently.The linear characteristics of ECG signals are extracted by convolution layers followed by Gaussian Error Linear Units(GeLu)and time series characteristics of ECG beats are extracted by Vanilla Long Short-Term Memory Networks(LSTM).Following on,the feature reduction of linear information is done with the help of ID Generalized Gated Pooling(GGP).In addition,data misbalancing issues are also addressed with the help of the Synthetic Minority Oversampling Technique(SMOTE).The performance assessment of the proposed model is done over the two publicly available datasets named MIT-BIH arrhythmia database(MITDB)and PTB Diagnostic ECG database(PTBDB).The proposed framework achieves an average accuracy performance of 99.14%along with a 95%recall value.展开更多
A mesoscale convective system(MCS) occurred over the East China coastal provinces and the East China Sea on 30April 2021, producing damaging surface winds near the coastal city Nantong with observed speeds reaching 45...A mesoscale convective system(MCS) occurred over the East China coastal provinces and the East China Sea on 30April 2021, producing damaging surface winds near the coastal city Nantong with observed speeds reaching 45 m s^(–1). A simulation using the Weather Research and Forecasting model with a 1.5-km grid spacing generally reproduces the development and subsequent organization of this convective system into an MCS, with an eastward protruding bow segment over the sea. In the simulation, an east-west-oriented high wind swath is generated behind the gust front of the MCS. Descending dry rear-to-front inflows behind the bow and trailing gust front are found to feed the downdrafts in the main precipitation regions. The inflows help to establish spreading cold outflows and enhance the downdrafts through evaporative cooling. Meanwhile, front-to-rear inflows from the south are present, associated with severely rearward-tilted updrafts initially forming over the gust front. Such inflows descend behind(north of) the gust front, significantly enhancing downdrafts and near-surface winds within the cold pool. Consistently, calculated trajectories show that these parcels that contribute to the derecho originate primarily from the region ahead(south) of the east-west-oriented gust front, and dry southwesterly flows in the low-to-middle levels contribute to strong downdrafts within the MCS. Moreover, momentum budget analyses reveal that a large westward-directed horizontal pressure gradient force within the simulated cold pool produced rapid flow acceleration towards Nantong. The analyses enrich the understanding of damaging wind characteristics over coastal East China and will prove helpful to operational forecasters.展开更多
The P24 antigen test, HIV RNA PCR test, HIV isolation/culture and fourth-generation HIV uniform Ag/Ab assay are being utilized in diagnosing acute HIV infection in different labs. Many factors limit the use of screeni...The P24 antigen test, HIV RNA PCR test, HIV isolation/culture and fourth-generation HIV uniform Ag/Ab assay are being utilized in diagnosing acute HIV infection in different labs. Many factors limit the use of screening for acute HIV in high-risk populations, in blood donors and during voluntary HIV testing, including, cost, technique, sensitivity and specificity. In this review we explore a new NAAT method which involves HIV RNA RT-PCR on pooled samples. This technique is able to screen for acute infections in a large testing volume and may be used as a screening method in high-risk populations and blood donors.展开更多
It is recognized that karst processes are actively involved in the current global carbon cycle based on twenty years research,and the carbon sink occurred in karst processes is possibly an important part of“missing s...It is recognized that karst processes are actively involved in the current global carbon cycle based on twenty years research,and the carbon sink occurred in karst processes is possibly an important part of“missing sink”in global carbon cycle.In this paper,an overview is given on karst carbon cycle research,and influence factors,formed carbon pools(background carbon sink)and sink increase potentials of current karst carbon cycle are analyzed.Carbonate weathering could contribute to the imbalance item(BIM)and land use change item(ELUC)in the global carbon cycle model,owing to its uptake of both atmospheric CO_(2)(carbon sink effect)and CO_(2) produced by soil respiration(carbon source reduction effect).Karst carbon sink includes inorganic carbon sink resulted from hydrogeochemical process and organic carbon sink generated by aquatic photosynthetic DIC conversion,forming relatively stable river(reservoir)water body or sediment carbon sink.The sizes of both sinks are controlled by terrestrial ecosystems and aquatic ecosystems,respectively.Desertification rehabilitation and carbon sequestration by aquatic plants are two effective ways to increase the carbon sink in karst area.It is estimated that the rate of carbon sink is at least 381000 t CO_(2)/a with vegetation restoration and afforestation in southwest China karst area,while the annual organic carbon sink generated by aquatic photosynthesis is about 84200 t C in the Pearl River Basin.The development of a soil CO_(2) based model for assessment of regional dissolution intensity will help to improve the estimation accuracy of carbon sink increase and potential,thus provide a more clear and efficient karst sink increase scheme and pathway to achieve the goals of“double carbon”.With the deep investigation on karst carbon cycle,mechanism and carbon sink effect,and the improvement of watershed carbon sink measurement methods and regional sink increase evaluation approaches.Karst carbon sink is expected to be included in the list of atmospheric CO_(2) sources/sinks of the global carbon budget in the near future.展开更多
Often in longitudinal studies, some subjects complete their follow-up visits, but others miss their visits due to various reasons. For those who miss follow-up visits, some of them might learn that the event of intere...Often in longitudinal studies, some subjects complete their follow-up visits, but others miss their visits due to various reasons. For those who miss follow-up visits, some of them might learn that the event of interest has already happened when they come back. In this case, not only are their event times interval-censored, but also their time-dependent measurements are incomplete. This problem was motivated by a national longitudinal survey of youth data. Maximum likelihood estimation (MLE) method based on expectation-maximization (EM) algorithm is used for parameter estimation. Then missing information principle is applied to estimate the variance-covariance matrix of the MLEs. Simulation studies demonstrate that the proposed method works well in terms of bias, standard error, and power for samples of moderate size. The national longitudinal survey of youth 1997 (NLSY97) data is analyzed for illustration.展开更多
The robust control law for gas tungsten arc welding dynamic process, which is a typical sampled-data system and full of uncertainties, is presented. By using the Lyapunov, second method, the robust control and robust ...The robust control law for gas tungsten arc welding dynamic process, which is a typical sampled-data system and full of uncertainties, is presented. By using the Lyapunov, second method, the robust control and robust optimal control for a class of sampled-data systems whose underlying continuous-time systems are subjected to structured uncertainties are discussed in time-domain. As a result, some sufficient conditions of robust stability and the corresponding robust control laws are derived. All these results are designed by solving a class of linear matrix inequalities （LMIs） and a class of dynamic optimization problem with LMIs constraints respectively. An example adapted under some experimental conditions in the dynamic process of gas tungsten arc welding system in which the controlled variable is the backside width of weld pool and controlling variable pulse duty ratio, is worked out to illustrate the proposed results, it is shown that the sampling period is the crucial design oarameter.展开更多
文摘Purpose: To assess the efficacy and safety of Olaparib, a PARP inhibitor on progression-free survival (PFS), objective response rate (ORR) and overall survival (OS) in patients with breast and ovarian cancer. Methods: Research data from clinical trials through PubMed, Science Citation Index, Elsevier Science Direct and Cochrane Library of all published studies exploring the PFS, ORR or OS of Olaparib for maintenance monotherapy on survival in breast and ovarian cancer were analysed. Pooled estimates of the ORR, weighted medians of PFS and OS from all Olaparib were calculated. Assessment of quality and level of evidence was assigned by Cochrane guidelines and guidelines of Oxford Centre for Evidence-Based Medicine. Results: Data of 893 patients (731 olaparib;162 control) from 6 trials, 2 randomised controlled trials and 4 non-randomised trials, were included. The overall median weighted PFS and OS in patients treated with Olaparib were 5.9 and 19.1 months, respectively. The pooled ORR was 25%. Olaparib showed a greater effect on PFS in patients with both wild-type BRCA and BRCA mutant gene. The most common toxicity were nausea and vomiting. Conclusions: Olaparib as maintenance monotherapy for breast and ovarian cancer is associated with promising outcomes including increased response rate and improved PFS. Its potential in clinical application is needed for further investigation in phase III trials.
文摘In studies reporting rugby league injuries,match injuries varied depending upon participation level.To review and update pooled data estimates for rugby league injury epidemiology and add information for participation levels in match and training environments.A systematic review and pooled analysis for published studies reporting rugby league match and training injuries.Searches were performed in the PubMed,CINHAL,ScienceDirect,Scopus,SPORTDiscus,SpringerLink,and Wiley Online databases.Studies were considered if they reported on rugby league match or training injuries between Jan 1990 to June 2021.Two authors(DK,TC)extracted the study characteristics,numerical data and assessed the article quality,by adhering to the protocol for systematic review of observational studies(MOOSE)and the STrengthening and Reporting of OBservational studies in Epidemiology(STROBE)statement.The 46 studies included a combined exposure of 419,037 h and 18,783 injuries incorporating 158,003 match-hr and 15,706 match injuries(99.4[95%CI:97.9–101.0]per 1000 match-hr)and 264,033 training-hr and 3077 training injuries(11.8[95%CI:11.4–12.2]per 1000 training-hr).Of included studies,47.9%utilised a medical attention/treatment injury definition.There was a five-fold difference in injuries for the semi-professional participation level(431.6 per 1000 match-hr)compared with professional(RR:4.92;p<0.001)and elite(RR:3.77;p<0.001)participation levels.The hooker recorded the highest pooled injury incidence(93.1 per 1000 match-hr).Compared to the 2014 analysis there was a 10-fold increase for headneck region(RR:10.7;p<0.001)injury incidence,and more injuries for the ball carrier(RR:1.1;p?0.008)and tackler(RR:1.2;p?0.001).There was a three-fold decrease in injury incidence in the first half(RR:2.9;p<0.001)and a two-fold decrease in the second half(RR:2.3;p<0.001)of matches.While rugby league match and training injury incidence had decreased since 2014,the increase in head injuries,and greater injury rate at the semi-professional level,mean further injury prevention interventions are needed.
基金supported by National Technology Support Project (2008BAD95B10-6)
文摘The organic carbon contents,carbon density and carbon storage of the soil in the Pinus koraiensis plantation ecosystem were investigated in Maoershan experimental forest farm,Shangzhi County,Heilongjiang,on the west slope of the Zhangguangcai Mountains in northeastern China for providing data to evaluation of the carbon balance in forest ecosystem of northeastern China.These soil carbon indicators were measured in three forest types,pure P.koraiensis plantation,P.koraiensis and Betula platyphylla mixed forest,and the P.koraiensis and Quercus mongolica mixed forest.The soil carbon pool consisted of four compartments,namely L layer,F layer,H layer and B layer.With variance analysis,we found that both organic carbon content and carbon density of the soil were significantly affected by forest types,soil compartments and slope positions.The highest soil carbon density（278.63 Mg·ha^-1）.was observed in the mixed forest of P.koraiensis and Q.mongolica.The B layer had the highest carbon density（212.28 Mg·ha^-1） among all the soil compartments.In terms of slope position,the highest soil carbon density（394.18 Mg·ha^-1） presented in the low slope.Besides,soil carbon content and carbon density had a marked change with the organic matter content and vertical depth of the soil in each compartment.The results of this study implied that in the temperate humid region,the mixed ecosystem of regional Pinus koraiensis plantations and natural forest had relatively high carbon storage capability.
基金Supported by Central University Basic Research Operating Expenses Special Fund(XDJK2011C026)Southwest University Doctoral Fund(09BSR04)~~
文摘[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.
基金supported by Shanghai Municipal Natural Science Foundation,China(No.21ZR1446800)the National Natural Science Foundation of China(No.41877425)the Fundamental Research Funds for the Central Universities(No.226-2024-00052)。
文摘Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community assembly in virgin and aging MP plastispheres across different habitats is poorly understood.This study aims to assess the variations in bacterial community assembly across different niches and habitats with an in situ ex-periment,in which constructed forest wetland(FW),natural lake wetland(LW),and lotus pond wetland(LP)were habitats,and plastispheres of virgin and aging low-density polyethylene(LDPE)MPs,as well as surround-ing wetland soils were niches.Significant niche-related differences in bacterial communities were observed,with lower diversity and enrichment of potential plastic-degrading bacteria in the plastisphere than in the soil bacterial communities.Furthermore,habitat-related differences exerted a more pronounced influence on the beta-diversity patterns of the bacterial communities.The linear regression analyses indicated that the local species pool con-tributed more to bacterial community assembly in the LW wetland,whereas the relative abundance of species was the primary factor in the LP wetland.The null model analysis indicated that plastisphere bacterial communi-ties were predominantly driven by the stochastic process,with a more deterministic assembly observed in the LP wetland and soil bacterial communities.Additionally,the primary ecological process shaping plastisphere com-munities shifted from drift in the virgin LDPE to homogenising dispersal in the aging LDPE.This study provides new insights into the fate and ecological impacts of MPs in wetlands,thereby facilitating the effective regulations of plastic pollution.
基金supported by National Institutes of Health(R35NS137480,R35NS116843,and RF1AG079557)by Dr.Miriam and Sheldon G.Adelson Medical Research Foundation.
文摘The adult subventricular zone of the lateral ventricles and the subgranular zone in the hippocampal dentate gyrus(DG)are the two brain regions where neurogenesis occurs throughout life in the adult mammalian brain(Ming and Song,2011).Adult quiescent hippocampal neural stem cells(NSCs)are bona fide stem cells and,when activated,give rise to newborn granule neurons in the adult brain,which play vital roles in learning,memory,mood,and affective cognition(Bonaguidi et al.,2011;Ming and Song,2011).
基金supported by the China Agriculture Research System of MOF and MARA(CARS-23-B04)the National Key Research and Development Program of China(2016YFD0201001)。
文摘Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.
文摘Based on the theory of formation dynamics of oil/gas pools, the Dongying sag can be divided into three dynamic systems regarding the accumulation of oil and gas： the superpressure closed system, the semi-closed system and the normal pressure open system. Based on the analysis of genesis of superpressure in the superpressure closed system and the rule of hydrocarbon expulsion, it is found that hydrocarbon generation is related to superpressure, which is the main driving factor of hydrocarbon migration. Micro fractures formed by superpressure are the main channels for hydrocarbon migration. There are three dynamic patterns for hydrocarbon expulsion： free water drainage, hydrocarbon accumulation and drainage through micro fissures. In the superpressure closed system, the oil-driving-water process and oil/gas accumulation were completed in lithologic traps by way of such two dynamic patterns as episodic evolution of superpressure systems and episodic pressure release of faults. The oil-bearing capacity of lithologic traps is intimately related to reservoir-forming dynamic force. Quantitative evaluation of dynamic conditions for pool formation can effectively predict the oil-bearing capability of traps.
基金the One-hundred Talents Program of CAS,the Special Fund for Agricultural Profession (200803030)the Sino-German project (DFG Training Group,GK1070)
文摘Atmospheric nitrogen (N) deposition has been poorly documented in northern China, an intensive agricultural and industrial region with large emissions of NHx and NOy. To quantify N deposition, total airborne N deposition was determined at three agricultural sites using a manual integrated total nitrogen input (ITNI) system during growth of winter wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) from September 2005 to May 2006. Total estimated N deposition averaged 54.9 and 43.2 kg N/hm2 across the three sites when wheat was grown to flowering and maturing, respectively. The average value was 50.2 kg N/hm2 when ryegrass was the indicator plant. Both indicator species gave similar total airborne N input results. The intermediate level of N supplied resulted in the highest N deposition, and the ratio of N acquired from deposition to total N content of the whole system decreased with increasing N supply to the roots. The contribution of atmospheric N to the total N content of the wheat and ryegrass sand culture systems ranged from 10% to 24%.
基金funded by the Korea Meteorological Institute (Grant No. KMI 2018-05410)
文摘A precipitation system developed continuously along the western coastline of the Korean Peninsula and created considerable precipitation both along the coast and inland on 26 July 2011. In this study, the causes for this nearshore convective system are investigated from observations and the results of model experiments. Three-dimensional radar fields clearly show that a change of wind at the surface border played an important role in the development of the nearshore convection system. The simulation results, which are very similar to the observations, show that the surface border generated and maintained the convergence zone. The roughness change enhanced the convergence, and the interaction between the deepening cold pool and downward flow maintained the convergence zone. The surface mechanical discontinuity affected by the roughness change between sea and land formed the convergence (gradient of wind stress), which induced momentum transfer to the upper layer. The cold pool created a steep gradient of potential temperature and provided the reason for the propagated convergence zone with the downward flow. The maximum value of the surface change factor, which comprises the influencing factors for the long-lasting convective system, reflects the enhancement of the system at the coast.
基金Under the auspices of Scientific and Technological Development Program of Jilin Province(No.20220101154JC)Strategic Pioneering Science and Technology Special Project of Chinese Academy of Sciences(No.XDA28080503)+1 种基金National Natural Science Foundation of China(No.42071025)Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2023240)。
文摘Building a more resilient response system to climate change for sustainable development and reducing uncertainty in China’s food markets,requires access to historical research gaps and mapping future research progress for decision making.However,the lack of quantitative and objective analyses to ensure the stability and development of agroecosystems increases the complexity of agro-climatic mechanisms,which leads to uncertainty and undesirable consequences.In this paper,we review the characteristics of climate change in China(1951–2020),reveal the mechanisms of agroecosystem structure in response to climate,and identify challenges and opportunities for future efforts in the context of research progress.The aim is to improve the scientific validity and relevance of future research by clarifying agro-climatic response mechanisms.The results show that surface temperature,precipitation,and frequency of extreme weather events have increased to varying degrees in major agricultural regions of China in 1951–2020.And they have strong geographic variation,which has resulted in droughts in the north and floods in the south.Moreover,climate change has complicated the mechanisms of soil moisture,Net Primary Productivity(NPP),soil carbon pool,and crop pest structure in agroecosystems.This lends to a reduction in soil water holding capacity,NPP,soil carbon content,and the number of natural enemies of diseases and insects,which in turn affects crop yields.However,human interventions can mitigate the deterioration of these factors.We have also realized that the methodology and theory of historical research poses a great challenge to future agroecosystem.Historical and projected climate trends identified current gaps in interdisciplinary integration and multidisciplinary research required to manage diverse spatio-temporal climate change impacts on agroecosystems.Future efforts should highlight integrated management and decision making,multidisciplinary big data coupling,and numerical simulations to ensure sustainable agricultural development,ecological security,and food security in China.
基金supported by the National Natural Science Foundation of China (Grant Nos.41730965, U2242204, and 41175047)the National Key Basic Research and Development Project of China (Grant No.2013CB430104)+2 种基金the Key Project of the Joint Funds of the Natural Science Foundation of Zhejiang Province (Grant No.LZJMZ23D050003financial support from the China Scholarship Council for her visit to CAPSUniversity of Oklahoma
文摘An extreme rainfall event occurred over Hangzhou,China,during the afternoon hours on 24 June 2013.This event occurred under suitable synoptic conditions and the maximum 4-h cumulative rainfall amount was over 150 mm.This rainfall event had two major rainbands.One was caused by a quasi-stationary convective line,and the other by a backbuilding convective line related to the interaction of the outflow boundary from the first rainband and an existing low-level mesoscale convergence line associated with a mei-yu frontal system.The rainfall event lasted 4 h,while the back-building process occurred in 2 h when the extreme rainfall center formed.So far,few studies have examined the back-building processes in the mei-yu season that are caused by the interaction of a mesoscale convergence line and a convective cold pool.The two rainbands are successfully reproduced by the Weather Research and Forecasting(WRF)model with fourlevel,two-way interactive nesting.In the model,new cells repeatedly occur at the west side of older cells,and the backbuilding process occurs in an environment with large CAPE,a low LFC,and plenty of water vapor.Outflows from older cells enhance the low-level convergence that forces new cells.High precipitation efficiency of the back-building training cells leads to accumulated precipitation of over 150 mm.Sensitivity experiments without evaporation of rainwater show that the convective cold pool plays an important role in the organization of the back-building process in the current extreme precipitation case.
文摘The continuous increase of human mobility combined with a relevant use of private vehicles contributes to increase the ill effects of vehicle externalities on the environment, e.g. high levels of air pollution, toxic emissions, noise pollution, and on the quality of life, e.g. parking problem, traffic congestion, and increase in the number of crashes and accidents. Transport demand management plays a very critical role in achieving greenhouse gas emission reduction targets. This study demonstrates that car pooling (CP) is an effective strategy to reduce transport volumes, transportation costs and related hill externalities in agreement with EU programs of emissions reduction targets. This paper presents an original approach to solve the CP problem. It is based on hierarchical clustering models, which have been adopted by an original decision support system (DSS). The DSS helps mobility managers to generate the pools and to design feasible paths for shared vehicles. A significant case studies and obtained results by the application of the proposed models are illustrated. They demonstrate the effectiveness of the approach and the supporting decisions tool.
基金supported by the MSIT(Ministry of Science and ICT),Korea,under the ITRC(Information Technology Research Center)Support Program(IITP-2023-2018-0-01799)supervised by the IITP(Institute for Information&Communications Technology Planning&Evaluation)and also the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2022R1F1A1063134).
文摘Effective smart healthcare frameworks contain novel and emerging solutions for remote disease diagnostics,which aid in the prevention of several diseases including heart-related abnormalities.In this context,regular monitoring of cardiac patients through smart healthcare systems based on Electrocardiogram(ECG)signals has the potential to save many lives.In existing studies,several heart disease diagnostic systems are proposed by employing different state-of-the-art methods,however,improving such methods is always an intriguing area of research.Hence,in this research,a smart healthcare system is proposed for the diagnosis of heart disease using ECG signals.The proposed framework extracts both linear and time-series information on the ECG signals and fuses them into a single framework concurrently.The linear characteristics of ECG signals are extracted by convolution layers followed by Gaussian Error Linear Units(GeLu)and time series characteristics of ECG beats are extracted by Vanilla Long Short-Term Memory Networks(LSTM).Following on,the feature reduction of linear information is done with the help of ID Generalized Gated Pooling(GGP).In addition,data misbalancing issues are also addressed with the help of the Synthetic Minority Oversampling Technique(SMOTE).The performance assessment of the proposed model is done over the two publicly available datasets named MIT-BIH arrhythmia database(MITDB)and PTB Diagnostic ECG database(PTBDB).The proposed framework achieves an average accuracy performance of 99.14%along with a 95%recall value.
基金primarily supported by the Ministry of Science and Technology of the People's Republic of China (MOST)(Grant No. 2018YFC1507303)National Natural Science Foundation of China (Grant Nos. 419505044,41941007, and 42230607)+1 种基金by the Talent Research Start-Up Fund of Nanjing University of Aeronautics and Astronautics(Grant No. 1007-90YAH22046)supported by The High Performance Computing Platform of Nanjing University of Aeronautics and Astronautics。
文摘A mesoscale convective system(MCS) occurred over the East China coastal provinces and the East China Sea on 30April 2021, producing damaging surface winds near the coastal city Nantong with observed speeds reaching 45 m s^(–1). A simulation using the Weather Research and Forecasting model with a 1.5-km grid spacing generally reproduces the development and subsequent organization of this convective system into an MCS, with an eastward protruding bow segment over the sea. In the simulation, an east-west-oriented high wind swath is generated behind the gust front of the MCS. Descending dry rear-to-front inflows behind the bow and trailing gust front are found to feed the downdrafts in the main precipitation regions. The inflows help to establish spreading cold outflows and enhance the downdrafts through evaporative cooling. Meanwhile, front-to-rear inflows from the south are present, associated with severely rearward-tilted updrafts initially forming over the gust front. Such inflows descend behind(north of) the gust front, significantly enhancing downdrafts and near-surface winds within the cold pool. Consistently, calculated trajectories show that these parcels that contribute to the derecho originate primarily from the region ahead(south) of the east-west-oriented gust front, and dry southwesterly flows in the low-to-middle levels contribute to strong downdrafts within the MCS. Moreover, momentum budget analyses reveal that a large westward-directed horizontal pressure gradient force within the simulated cold pool produced rapid flow acceleration towards Nantong. The analyses enrich the understanding of damaging wind characteristics over coastal East China and will prove helpful to operational forecasters.
文摘The P24 antigen test, HIV RNA PCR test, HIV isolation/culture and fourth-generation HIV uniform Ag/Ab assay are being utilized in diagnosing acute HIV infection in different labs. Many factors limit the use of screening for acute HIV in high-risk populations, in blood donors and during voluntary HIV testing, including, cost, technique, sensitivity and specificity. In this review we explore a new NAAT method which involves HIV RNA RT-PCR on pooled samples. This technique is able to screen for acute infections in a large testing volume and may be used as a screening method in high-risk populations and blood donors.
基金supported by China national key research and development program (2020YFE0204 700)China aid project of MOST (KY201802009)+1 种基金Science and technology project of Guangxi (Guike AD17129047)International cooperation project of CGS (132852KYSB20170029-01)。
文摘It is recognized that karst processes are actively involved in the current global carbon cycle based on twenty years research,and the carbon sink occurred in karst processes is possibly an important part of“missing sink”in global carbon cycle.In this paper,an overview is given on karst carbon cycle research,and influence factors,formed carbon pools(background carbon sink)and sink increase potentials of current karst carbon cycle are analyzed.Carbonate weathering could contribute to the imbalance item(BIM)and land use change item(ELUC)in the global carbon cycle model,owing to its uptake of both atmospheric CO_(2)(carbon sink effect)and CO_(2) produced by soil respiration(carbon source reduction effect).Karst carbon sink includes inorganic carbon sink resulted from hydrogeochemical process and organic carbon sink generated by aquatic photosynthetic DIC conversion,forming relatively stable river(reservoir)water body or sediment carbon sink.The sizes of both sinks are controlled by terrestrial ecosystems and aquatic ecosystems,respectively.Desertification rehabilitation and carbon sequestration by aquatic plants are two effective ways to increase the carbon sink in karst area.It is estimated that the rate of carbon sink is at least 381000 t CO_(2)/a with vegetation restoration and afforestation in southwest China karst area,while the annual organic carbon sink generated by aquatic photosynthesis is about 84200 t C in the Pearl River Basin.The development of a soil CO_(2) based model for assessment of regional dissolution intensity will help to improve the estimation accuracy of carbon sink increase and potential,thus provide a more clear and efficient karst sink increase scheme and pathway to achieve the goals of“double carbon”.With the deep investigation on karst carbon cycle,mechanism and carbon sink effect,and the improvement of watershed carbon sink measurement methods and regional sink increase evaluation approaches.Karst carbon sink is expected to be included in the list of atmospheric CO_(2) sources/sinks of the global carbon budget in the near future.
文摘Often in longitudinal studies, some subjects complete their follow-up visits, but others miss their visits due to various reasons. For those who miss follow-up visits, some of them might learn that the event of interest has already happened when they come back. In this case, not only are their event times interval-censored, but also their time-dependent measurements are incomplete. This problem was motivated by a national longitudinal survey of youth data. Maximum likelihood estimation (MLE) method based on expectation-maximization (EM) algorithm is used for parameter estimation. Then missing information principle is applied to estimate the variance-covariance matrix of the MLEs. Simulation studies demonstrate that the proposed method works well in terms of bias, standard error, and power for samples of moderate size. The national longitudinal survey of youth 1997 (NLSY97) data is analyzed for illustration.
基金This project is supported by Doctor's Research Fund of Science Education Ministry of China(No.20060214004)Scientific Research Fund Education Ministry of China(No.206041)Scientific Research Fund of Harbin Sci-ence Bureau China(No.20051AAICG037).
文摘The robust control law for gas tungsten arc welding dynamic process, which is a typical sampled-data system and full of uncertainties, is presented. By using the Lyapunov, second method, the robust control and robust optimal control for a class of sampled-data systems whose underlying continuous-time systems are subjected to structured uncertainties are discussed in time-domain. As a result, some sufficient conditions of robust stability and the corresponding robust control laws are derived. All these results are designed by solving a class of linear matrix inequalities （LMIs） and a class of dynamic optimization problem with LMIs constraints respectively. An example adapted under some experimental conditions in the dynamic process of gas tungsten arc welding system in which the controlled variable is the backside width of weld pool and controlling variable pulse duty ratio, is worked out to illustrate the proposed results, it is shown that the sampling period is the crucial design oarameter.
