The aim of the present study was to investigate the effects of various calcium salt solutions(calcium chloride,formate,acetate,citrate)on the cultivation,mineral profile,and polyphenol composition of common vetch(Vici...The aim of the present study was to investigate the effects of various calcium salt solutions(calcium chloride,formate,acetate,citrate)on the cultivation,mineral profile,and polyphenol composition of common vetch(Vicia sativa L.)sprouts.The results demonstrated that low concentrations(0.5 mM)of organic calcium salts,particularly calcium acetate,significantly promoted stem length,biomass accumulation,and calcium enrichment without adversely affecting other minerals like potassium and magnesium.In contrast,inorganic calcium chloride exhibited antagonistic effects on magnesium absorption.While enhancing total polyphenol content,calcium chloride increased oxidative stress markers like malondialdehyde and electrolyte leakage.Conversely,organic calcium treatments alleviated oxidative damage and reduced the activity of antioxidant enzymes like peroxidase and superoxide dismutase,suggesting efficient stress management.Organic calcium sources also modulated the polyphenols composition,decreasing luteolin but enhancing specific polyphenols like gallic,ferulic,syringic,and apigenin.Gene expression analysis revealed a general downregulation of key phenylpropanoid pathway enzymes(phenylalanine ammonia-lyase,ferulate-5-hydroxylase,and 4-coumarate-CoA ligase)under calcium treatments,potentially contributing to the observed changes in polyphenol profiles.These findings highlight the potential of using organic calcium salts to enhance the nutritional value of common vetch sprouts.展开更多
基金supported by the grants from China Agriculture Research System-Green Manure(Grant No.31771964).
文摘The aim of the present study was to investigate the effects of various calcium salt solutions(calcium chloride,formate,acetate,citrate)on the cultivation,mineral profile,and polyphenol composition of common vetch(Vicia sativa L.)sprouts.The results demonstrated that low concentrations(0.5 mM)of organic calcium salts,particularly calcium acetate,significantly promoted stem length,biomass accumulation,and calcium enrichment without adversely affecting other minerals like potassium and magnesium.In contrast,inorganic calcium chloride exhibited antagonistic effects on magnesium absorption.While enhancing total polyphenol content,calcium chloride increased oxidative stress markers like malondialdehyde and electrolyte leakage.Conversely,organic calcium treatments alleviated oxidative damage and reduced the activity of antioxidant enzymes like peroxidase and superoxide dismutase,suggesting efficient stress management.Organic calcium sources also modulated the polyphenols composition,decreasing luteolin but enhancing specific polyphenols like gallic,ferulic,syringic,and apigenin.Gene expression analysis revealed a general downregulation of key phenylpropanoid pathway enzymes(phenylalanine ammonia-lyase,ferulate-5-hydroxylase,and 4-coumarate-CoA ligase)under calcium treatments,potentially contributing to the observed changes in polyphenol profiles.These findings highlight the potential of using organic calcium salts to enhance the nutritional value of common vetch sprouts.
