Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we u...Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we used transdermal enhancers to deliverAngong Niuhuang pill by modern technology, which expanded the safe dose range and clinical indications. In this study, Angong Niuhuang stickers administered at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to theDazhui （DU14）, Qihai（RN6） andMingmen （DU4） of rats with chronic cerebral ischemia, for 4 weeks. The Morris water maze was used to determine the learning and memory ability of rats. Hematoxylin-eosin staining and Nissl staining were used to observe neuronal damage of the cortex and hippocampal CA1 region in rats with chronic cerebral ischemia. The middle- and high-dose point application ofAngong Niuhuangstickers attenuated neuronal damage in the cortex and hippocampal CA1 region, and improved the memory of rats with chronic cerebral ischemia with an efifcacy similar to interventions by electroacupuncture at Dazhui （DU14）,Qihai （RN6） andMingmen （DU4）. Our experimental ifndings indicate that point application withAngong Niuhuang stickers can improve cognitive function after chronic cerebral ischemia in rats and is neuroprotective with an equivalent efifcacy to acupuncture.展开更多
In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts fr...In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to Dazhui （DU14）, Qihai （RN6） and Mingmen （DU4）. Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia.展开更多
OBJECTIVE:To optimise point application therapy for the treatment of asthma.METHODS:A total of 456 patients with asthma were randomly divided into treatment groups 1,2,and 3,with 152 patients each.The Dazhui(GV14),Fei...OBJECTIVE:To optimise point application therapy for the treatment of asthma.METHODS:A total of 456 patients with asthma were randomly divided into treatment groups 1,2,and 3,with 152 patients each.The Dazhui(GV14),Feishu(BL13),Xinshu(BL15),Geshu(BL17),Pishu(BL20),Shenshu(BL23),and Guanyuan(CV4)acupoints were selected for treatment groups 1 and 2,with 2 and 4 h of application,respectively.The Feishu(BL13),Xinshu(BL15),and Geshu(BL17)acupoints were selected in group 3,with 2 h of application.All patients underwent application of the“prevention of disease”plaster during the dog days of summer(approximately July and August each year)between 2015 and 2019.RESULTS:The analysis showed that the numbers of asthma attacks and total serum immunoglobulin E(IgE)levels of the three groups after treatment were significantly lower than those before treatment.In addition,the reductions in treatment groups 1 and 2 were more significant than those in group 3.The total treatment efficacy rates and post-treatment quality of life scores of the patients in treatment groups 1 and 2 were significantly higher than those in group 3.CONCLUSIONS:Point application therapy can effectively improve the clinical symptoms of patients with asthma,reduce the number of attacks,improve the quality of life,and decrease the total serum IgE level.The choice of acupuncture points,but not the length of the application time,influenced the therapeutic effect of point application therapy in asthma.展开更多
Objective:To observe the clinical efficacy of subcutaneous row needling at the Foot Motor Sensory Area combined with point application therapy and its influence on defecation-related symptoms.Methods:Seventy eligible ...Objective:To observe the clinical efficacy of subcutaneous row needling at the Foot Motor Sensory Area combined with point application therapy and its influence on defecation-related symptoms.Methods:Seventy eligible patients with poststroke constipation were assigned to an observation group or a control group using the random number table method,with 35 patients in each group.The observation group received subcutaneous row needling at the Foot Motor Sensory Area combined with point application therapy,whereas the control group received lactulose oral solution.After treatment,the total effective rate and safety were compared between the two groups,and changes in the patient assessment of constipation symptom(PAC-SYM),stool form,and constipation symptom scores were observed.Results:The total effective rate was 87.9%in the observation group and 69.7%in the control group,and the difference was statistically significant(P<0.05).No obvious adverse reactions occurred in either group.After treatment,PAC-SYM and constipation symptom scores in both groups were lower than before treatment(P<0.05),with the observation group scoring lower than the control group(P<0.05).After treatment,the Bristol stool form scale(BSFS)score in both groups increased(P<0.05)and approached the score of normal stool form;the difference in the BSFS score between the two groups was not statistically significant(P>0.05).Conclusion:Compared to oral lactulose,subcutaneous insertion with row needling at the scalp Foot Motor Sensory Area combined with point application therapy can better improve poststroke constipation symptoms,shorten both the interval between bowel movements and the duration of each bowel movement,and reduce rectal and abdominal discomfort,with fewer adverse reactions and good safety.展开更多
Objective： To observe the clinical effect of point application using Chinese herbal medicine for chronic cough in children. Methods： A total of 200 children with chronic cough were randomly allocated into an observ...Objective： To observe the clinical effect of point application using Chinese herbal medicine for chronic cough in children. Methods： A total of 200 children with chronic cough were randomly allocated into an observation group and a control group, 100 cases in each group. Conventional Western medication was used for cases in the control group, whereas conventional Western medication plus point application using Chinese herbal medicine were used for cases in the observation group. Changes of indexes including eosinophils （EOS）, peak expiratory flow （PEF） and forced expiratory volume in one second （FEV1） were observed before and after treatment. In addition, the clinical effects were compared between the two groups. Results： After treatment, coughs in children were alleviated in both groups; however, it took a shorter period of time for cough alleviation in the observation group than that in the control group （P^0.01）. On the 28th day of treatment, the EOS, PEF and FEVl were improved in both groups （P〈0.01）, and the improvements were more significant in the observation group （P〈0.05）. Additionally, the frequencies of cough variant asthma attacks were decreased in both groups, but a better effect in the observation group than the control group. Conclusion： Complementary point application using Chinese herbal medicine could substantially improve the clinical effect, alleviate coughs and benefit lung functions.展开更多
Both Huiyang (BL 35) and Zhonglushu (BL 29) are the acupoints of the Bladder Meridian of Foot-Taiyang located in the sacral region. These two points are often used in combination to treat urinary dysfunction, and ... Both Huiyang (BL 35) and Zhonglushu (BL 29) are the acupoints of the Bladder Meridian of Foot-Taiyang located in the sacral region. These two points are often used in combination to treat urinary dysfunction, and their regulatory effects are very satisfactory.1 In this article, the clinical mechanism of Huiyang and Zhonglushu is explained in accordance with the theory of Channels and neurophysiology.
……展开更多
Taichong (LR 3) is a yuan (source) point of the Liver Channel of Foot Taiyin. Having the effects of relieving the depressed liver, and regulating the circulation of qi to relieve pain, this point is widely used in cli...Taichong (LR 3) is a yuan (source) point of the Liver Channel of Foot Taiyin. Having the effects of relieving the depressed liver, and regulating the circulation of qi to relieve pain, this point is widely used in clinic with quite satisfactory therapeutic effects. The following is a report of example cases.展开更多
Wangu (GB 12) is a point of the Gallbladder Channel of Foot Shaoyang, located in the depression posteroinferior to the temporal process. And it is a hui point of Foot Taiyang and Shaoyang Channels, with the effect... Wangu (GB 12) is a point of the Gallbladder Channel of Foot Shaoyang, located in the depression posteroinferior to the temporal process. And it is a hui point of Foot Taiyang and Shaoyang Channels, with the effects of eliminating pathogenic wind and heat, and tranquilizing the mind. This point is located superior to the attachment point of the sternocleidomastoid muscle, in which there distribute the stem of the lesser occipital nerve, and branches of the posterior auricular artery and vein. It can be needled perpendicularly 0.5-1 cun deep. In clinical practice, the author has applied the point for treatment of various diseases, and obtained quite satisfactory therapeutic results. The following is a report of some illustrative cases. ……展开更多
In recent years, based on the experience of predecessors, the authors of this article have applied Zhongzhu point (TE 3) in treatment for various diseases and obtained satisfactory therapeutic results. Some of the... In recent years, based on the experience of predecessors, the authors of this article have applied Zhongzhu point (TE 3) in treatment for various diseases and obtained satisfactory therapeutic results. Some of the illustrative cases are introduced below.……展开更多
The authors of this article have successfully treated various cases of urinary dysfunction by means of acupuncture. Some of the cases are reported in the following.
Zhaohai (KI 6) is a point on the Kidney Channel of Foot Shaoyin, and it is one of the eight confluent points, connecting with the Yinqiao Channel. It is located in the depression of the lower border of the medial mall...Zhaohai (KI 6) is a point on the Kidney Channel of Foot Shaoyin, and it is one of the eight confluent points, connecting with the Yinqiao Channel. It is located in the depression of the lower border of the medial malleolus, indicated for the disorders along the distributing courses of the Foot Shaoyin Channel and Yinqiao Channel. Connecting with the kidney and bladder collaterally, it gives the effect of reinforcing the kidney and strengthening qi, and promoting the function of the bladder.展开更多
Using a recent result regarding the fixed points of multivalued mappings, the existence of invariant best simultaneous approximation in chainable metric space is proved.
Based on the Coulomb's theory that the earth pressure against the back of a retaining wall is due to the thrust exerted by the sliding wedge of soil from the back of the wall to a plane which passes through the bo...Based on the Coulomb's theory that the earth pressure against the back of a retaining wall is due to the thrust exerted by the sliding wedge of soil from the back of the wall to a plane which passes through the bottom edge of the wall and has an inclination equal to the angle of θ, the theoretical answers to the unit earth pressure, the resultant earth pressure and the point of application of the resultant earth pressure on a retaining wall were obtained for the wall movement mode of rotation about top. The comparisons were made among the formula presented here, the formula for the wall movement mode of translation, the Coulomb's formula and some experimental observations. It is demonstrated that the magnitudes of the resultant earth pressures for the wall movement mode of rotation about top is equal to that determined by the formula for the wall movement mode of translation and the Coulomb's theory. But the distribution of the earth pressure and the points of application of the resultant earth pressures have significant difference.展开更多
Under the background of the new situation, agricultural technology has ample room for improvement due to the rapid development of science and technology and economy, among which high-yield cultivation technology of wh...Under the background of the new situation, agricultural technology has ample room for improvement due to the rapid development of science and technology and economy, among which high-yield cultivation technology of wheat is widely used. From the perspective of cultivation experience, high yield is the research made by discovering the planting rule and production rule of wheat and combining with scientific theory to promote the development of high-quality and high-quality wheat crops.展开更多
At this stage, with the deepening of China's urbanization process, transportation is an important carrier of urban economic development. Highway maintenance treatment, as an important link, is the primary conditio...At this stage, with the deepening of China's urbanization process, transportation is an important carrier of urban economic development. Highway maintenance treatment, as an important link, is the primary condition to ensure the long-term sustainable development of highway engineering projects. Through preventive maintenance treatment, the highway maintenance project can achieve the ideal effect. This paper mainly discusses the application of preventive maintenance technology in highway maintenance engineering in order to improve the level of highway maintenance.展开更多
Since the onset of the HIV epidemic,assessing CD4+T-cells has become a routine procedure for evaluating immune deficiency,with flow cytometry established as the gold standard.Over time,various strategies and platforms...Since the onset of the HIV epidemic,assessing CD4+T-cells has become a routine procedure for evaluating immune deficiency,with flow cytometry established as the gold standard.Over time,various strategies and platforms have been introduced to improve CD4+cell enumeration,aiming to enhance the performance of diagnostic devices and bring the service closer to patients.These advancements are particularly critical for low-resource settings and point-of-care applications,where the excellent performance of flow cytometry is hindered by its unsuitability in such environments.This work presents an innovative electrochemical microfluidic device that,with further development,could be applied for HIV management in low resource settings.The setup integrates an electrochemical sensor within a PDMS microfluidic structure,allowing for on-chip electrode functionalization and cell detection.Using electrochemical impedance spectroscopy,the biosensor demonstrates a linear detection range from 1.25×105 to 2×106 cells/mL,with a detection limit of 1.41×105 cells/mL for CD4+cells isolated from blood samples,aligning with clinical ranges for both healthy and HIV+patients.The biosensor shows specificity towards CD4+cells with negligible response to monocytes,neutrophils,and bovine serum albumin.Its integration with a microfluidic chip for sensor fabrication and cell detection,compact size,minimal manual handling,ease of fabrication,electrochemical detection capability,and potential for multiplexing together with the detection range make the device particularly advantageous for use in low-resource settings,standing out among other devices described in the literature.This study also investigates the integration of a microfluidic Dean Flow Fractionation(DFF)chip for cell separation.展开更多
基金supported by the National Natural Science Foundation of China,No.81403458a grant from Cultivation Project Foundation for Youth Technological Talents of Southern Medical University,No.B1012015
文摘Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we used transdermal enhancers to deliverAngong Niuhuang pill by modern technology, which expanded the safe dose range and clinical indications. In this study, Angong Niuhuang stickers administered at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to theDazhui （DU14）, Qihai（RN6） andMingmen （DU4） of rats with chronic cerebral ischemia, for 4 weeks. The Morris water maze was used to determine the learning and memory ability of rats. Hematoxylin-eosin staining and Nissl staining were used to observe neuronal damage of the cortex and hippocampal CA1 region in rats with chronic cerebral ischemia. The middle- and high-dose point application ofAngong Niuhuangstickers attenuated neuronal damage in the cortex and hippocampal CA1 region, and improved the memory of rats with chronic cerebral ischemia with an efifcacy similar to interventions by electroacupuncture at Dazhui （DU14）,Qihai （RN6） andMingmen （DU4）. Our experimental ifndings indicate that point application withAngong Niuhuang stickers can improve cognitive function after chronic cerebral ischemia in rats and is neuroprotective with an equivalent efifcacy to acupuncture.
基金supported by the Research Project of Administration of TCM of Guangdong Province, No. 20111270
文摘In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to Dazhui （DU14）, Qihai （RN6） and Mingmen （DU4）. Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia.
基金Supported by Major Science and Technology Special Project of Zhejiang Province:Research and Application of Key Techniques of Point Application Therapy for Treating Winter Diseases and Summer Diseases to Prevent and Treat Respiratory Allergic Diseases(No.2014C03046-2)the Young Scientists of National Natural Science Foundation of China:Study on the Mechanism of Point Application Therapy Regulating Autophagy of Airway Epithelial Cells in the Treatment of Asthma Based on AMPK/mTOR Signal Pathway,(No.82004454)Zhejiang Traditional Chinese Medicine Administration:based on the Theory of"Lung Intestine Axis",a Comparative Study on the Therapeutic Effect of Different Point Application Therapy on Patients with AnaphyLactic Asthma in Remission Stage(No.2021ZQ035)。
文摘OBJECTIVE:To optimise point application therapy for the treatment of asthma.METHODS:A total of 456 patients with asthma were randomly divided into treatment groups 1,2,and 3,with 152 patients each.The Dazhui(GV14),Feishu(BL13),Xinshu(BL15),Geshu(BL17),Pishu(BL20),Shenshu(BL23),and Guanyuan(CV4)acupoints were selected for treatment groups 1 and 2,with 2 and 4 h of application,respectively.The Feishu(BL13),Xinshu(BL15),and Geshu(BL17)acupoints were selected in group 3,with 2 h of application.All patients underwent application of the“prevention of disease”plaster during the dog days of summer(approximately July and August each year)between 2015 and 2019.RESULTS:The analysis showed that the numbers of asthma attacks and total serum immunoglobulin E(IgE)levels of the three groups after treatment were significantly lower than those before treatment.In addition,the reductions in treatment groups 1 and 2 were more significant than those in group 3.The total treatment efficacy rates and post-treatment quality of life scores of the patients in treatment groups 1 and 2 were significantly higher than those in group 3.CONCLUSIONS:Point application therapy can effectively improve the clinical symptoms of patients with asthma,reduce the number of attacks,improve the quality of life,and decrease the total serum IgE level.The choice of acupuncture points,but not the length of the application time,influenced the therapeutic effect of point application therapy in asthma.
文摘Objective:To observe the clinical efficacy of subcutaneous row needling at the Foot Motor Sensory Area combined with point application therapy and its influence on defecation-related symptoms.Methods:Seventy eligible patients with poststroke constipation were assigned to an observation group or a control group using the random number table method,with 35 patients in each group.The observation group received subcutaneous row needling at the Foot Motor Sensory Area combined with point application therapy,whereas the control group received lactulose oral solution.After treatment,the total effective rate and safety were compared between the two groups,and changes in the patient assessment of constipation symptom(PAC-SYM),stool form,and constipation symptom scores were observed.Results:The total effective rate was 87.9%in the observation group and 69.7%in the control group,and the difference was statistically significant(P<0.05).No obvious adverse reactions occurred in either group.After treatment,PAC-SYM and constipation symptom scores in both groups were lower than before treatment(P<0.05),with the observation group scoring lower than the control group(P<0.05).After treatment,the Bristol stool form scale(BSFS)score in both groups increased(P<0.05)and approached the score of normal stool form;the difference in the BSFS score between the two groups was not statistically significant(P>0.05).Conclusion:Compared to oral lactulose,subcutaneous insertion with row needling at the scalp Foot Motor Sensory Area combined with point application therapy can better improve poststroke constipation symptoms,shorten both the interval between bowel movements and the duration of each bowel movement,and reduce rectal and abdominal discomfort,with fewer adverse reactions and good safety.
基金supported by Xu’s Pediatric Clinical Experience Inheritance Base (No. ZYSNXD-CC-HPGCJD-005)Emerging Frontier Technology Joint Research Program of Shanghai Municipal Hospitals (No. SHDC12012410)+3 种基金Domestic Visiting Scholar Program for Young Teachers at Shanghai Colleges and UniversitiesTang Wei-yong Work Room of Shanghai Three-year Action Plan of Traditional Chinese Medicine (No. ZYSNXD-CC-MZY001)Cheng Jia-zheng Work Room of Three-year Action Plan of Traditional Chinese Medicine (No. ZYSNXD-CC-MZY002)the Third Group Construction Project of Clinical Advantages Specialized Subject of Traditional Chinese Medicine in Shanghai (No. ZYSNXD-YL-YSZK006)
文摘Objective： To observe the clinical effect of point application using Chinese herbal medicine for chronic cough in children. Methods： A total of 200 children with chronic cough were randomly allocated into an observation group and a control group, 100 cases in each group. Conventional Western medication was used for cases in the control group, whereas conventional Western medication plus point application using Chinese herbal medicine were used for cases in the observation group. Changes of indexes including eosinophils （EOS）, peak expiratory flow （PEF） and forced expiratory volume in one second （FEV1） were observed before and after treatment. In addition, the clinical effects were compared between the two groups. Results： After treatment, coughs in children were alleviated in both groups; however, it took a shorter period of time for cough alleviation in the observation group than that in the control group （P^0.01）. On the 28th day of treatment, the EOS, PEF and FEVl were improved in both groups （P〈0.01）, and the improvements were more significant in the observation group （P〈0.05）. Additionally, the frequencies of cough variant asthma attacks were decreased in both groups, but a better effect in the observation group than the control group. Conclusion： Complementary point application using Chinese herbal medicine could substantially improve the clinical effect, alleviate coughs and benefit lung functions.
文摘 Both Huiyang (BL 35) and Zhonglushu (BL 29) are the acupoints of the Bladder Meridian of Foot-Taiyang located in the sacral region. These two points are often used in combination to treat urinary dysfunction, and their regulatory effects are very satisfactory.1 In this article, the clinical mechanism of Huiyang and Zhonglushu is explained in accordance with the theory of Channels and neurophysiology.
……
文摘Taichong (LR 3) is a yuan (source) point of the Liver Channel of Foot Taiyin. Having the effects of relieving the depressed liver, and regulating the circulation of qi to relieve pain, this point is widely used in clinic with quite satisfactory therapeutic effects. The following is a report of example cases.
文摘 Wangu (GB 12) is a point of the Gallbladder Channel of Foot Shaoyang, located in the depression posteroinferior to the temporal process. And it is a hui point of Foot Taiyang and Shaoyang Channels, with the effects of eliminating pathogenic wind and heat, and tranquilizing the mind. This point is located superior to the attachment point of the sternocleidomastoid muscle, in which there distribute the stem of the lesser occipital nerve, and branches of the posterior auricular artery and vein. It can be needled perpendicularly 0.5-1 cun deep. In clinical practice, the author has applied the point for treatment of various diseases, and obtained quite satisfactory therapeutic results. The following is a report of some illustrative cases. ……
文摘 In recent years, based on the experience of predecessors, the authors of this article have applied Zhongzhu point (TE 3) in treatment for various diseases and obtained satisfactory therapeutic results. Some of the illustrative cases are introduced below.……
文摘The authors of this article have successfully treated various cases of urinary dysfunction by means of acupuncture. Some of the cases are reported in the following.
文摘Zhaohai (KI 6) is a point on the Kidney Channel of Foot Shaoyin, and it is one of the eight confluent points, connecting with the Yinqiao Channel. It is located in the depression of the lower border of the medial malleolus, indicated for the disorders along the distributing courses of the Foot Shaoyin Channel and Yinqiao Channel. Connecting with the kidney and bladder collaterally, it gives the effect of reinforcing the kidney and strengthening qi, and promoting the function of the bladder.
文摘Using a recent result regarding the fixed points of multivalued mappings, the existence of invariant best simultaneous approximation in chainable metric space is proved.
文摘Based on the Coulomb's theory that the earth pressure against the back of a retaining wall is due to the thrust exerted by the sliding wedge of soil from the back of the wall to a plane which passes through the bottom edge of the wall and has an inclination equal to the angle of θ, the theoretical answers to the unit earth pressure, the resultant earth pressure and the point of application of the resultant earth pressure on a retaining wall were obtained for the wall movement mode of rotation about top. The comparisons were made among the formula presented here, the formula for the wall movement mode of translation, the Coulomb's formula and some experimental observations. It is demonstrated that the magnitudes of the resultant earth pressures for the wall movement mode of rotation about top is equal to that determined by the formula for the wall movement mode of translation and the Coulomb's theory. But the distribution of the earth pressure and the points of application of the resultant earth pressures have significant difference.
文摘Under the background of the new situation, agricultural technology has ample room for improvement due to the rapid development of science and technology and economy, among which high-yield cultivation technology of wheat is widely used. From the perspective of cultivation experience, high yield is the research made by discovering the planting rule and production rule of wheat and combining with scientific theory to promote the development of high-quality and high-quality wheat crops.
文摘At this stage, with the deepening of China's urbanization process, transportation is an important carrier of urban economic development. Highway maintenance treatment, as an important link, is the primary condition to ensure the long-term sustainable development of highway engineering projects. Through preventive maintenance treatment, the highway maintenance project can achieve the ideal effect. This paper mainly discusses the application of preventive maintenance technology in highway maintenance engineering in order to improve the level of highway maintenance.
基金funding from Santander postgraduate mobility awards and Department of Electronic&Electrical Engineering,University of BathR.S.was funded through UK Engineering and Physical Sciences Research Council grant number EP/V040189/1.
文摘Since the onset of the HIV epidemic,assessing CD4+T-cells has become a routine procedure for evaluating immune deficiency,with flow cytometry established as the gold standard.Over time,various strategies and platforms have been introduced to improve CD4+cell enumeration,aiming to enhance the performance of diagnostic devices and bring the service closer to patients.These advancements are particularly critical for low-resource settings and point-of-care applications,where the excellent performance of flow cytometry is hindered by its unsuitability in such environments.This work presents an innovative electrochemical microfluidic device that,with further development,could be applied for HIV management in low resource settings.The setup integrates an electrochemical sensor within a PDMS microfluidic structure,allowing for on-chip electrode functionalization and cell detection.Using electrochemical impedance spectroscopy,the biosensor demonstrates a linear detection range from 1.25×105 to 2×106 cells/mL,with a detection limit of 1.41×105 cells/mL for CD4+cells isolated from blood samples,aligning with clinical ranges for both healthy and HIV+patients.The biosensor shows specificity towards CD4+cells with negligible response to monocytes,neutrophils,and bovine serum albumin.Its integration with a microfluidic chip for sensor fabrication and cell detection,compact size,minimal manual handling,ease of fabrication,electrochemical detection capability,and potential for multiplexing together with the detection range make the device particularly advantageous for use in low-resource settings,standing out among other devices described in the literature.This study also investigates the integration of a microfluidic Dean Flow Fractionation(DFF)chip for cell separation.
