Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. ...Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield and content, such as extracting temperature, extracting time, the ratio of water to defatted kernel and concentration of ethanol were analyzed under specific condition. The optimal extracting parameters for ultrasound-associated extraction were determined as the ultrasonic temperature 70℃, the ratio of defatted kernel to water 1：20, the extracting time 40 min, and ethanol concentration 80%. Under such extraction conditions, the yield of water soluble polysaccharide was 3.65% and the average content of polysaccharide was 45.38% in the raw polysaccharides which gained in the experiment. Both extraction yield and content of polysaccharides extracted with ultrasound-associated extraction were higher than that with hot water extraction. The study demonstrates that ultrasound is a reliable and extremely effective tool for the fast extraction of water soluble polysaccharide of Korean pine kernel.展开更多
用微晶纤维素层析法和双波长薄层锯形扫描法,对红松(Pinus koraiensis Sieb. et Zucc)籽仁的氨基酸成份和含量进行定性和定量分析,分析结果:红松籽仁中至少含有17种氨基酸,其含量最小的为酪氨酸(0.37％),最大为谷氨酸(7.96％),贮藏时间...用微晶纤维素层析法和双波长薄层锯形扫描法,对红松(Pinus koraiensis Sieb. et Zucc)籽仁的氨基酸成份和含量进行定性和定量分析,分析结果:红松籽仁中至少含有17种氨基酸,其含量最小的为酪氨酸(0.37％),最大为谷氨酸(7.96％),贮藏时间对红松籽仁氨基酸含量有明显影响,大部分种类氨基酸的含量随贮藏时间增加而减少,红松种仁含氨基酸总量比苏联西伯利亚红松(P.sibirca)籽仁含氨基酸总量高7.64％。展开更多
文摘Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield and content, such as extracting temperature, extracting time, the ratio of water to defatted kernel and concentration of ethanol were analyzed under specific condition. The optimal extracting parameters for ultrasound-associated extraction were determined as the ultrasonic temperature 70℃, the ratio of defatted kernel to water 1：20, the extracting time 40 min, and ethanol concentration 80%. Under such extraction conditions, the yield of water soluble polysaccharide was 3.65% and the average content of polysaccharide was 45.38% in the raw polysaccharides which gained in the experiment. Both extraction yield and content of polysaccharides extracted with ultrasound-associated extraction were higher than that with hot water extraction. The study demonstrates that ultrasound is a reliable and extremely effective tool for the fast extraction of water soluble polysaccharide of Korean pine kernel.
文摘用微晶纤维素层析法和双波长薄层锯形扫描法,对红松(Pinus koraiensis Sieb. et Zucc)籽仁的氨基酸成份和含量进行定性和定量分析,分析结果:红松籽仁中至少含有17种氨基酸,其含量最小的为酪氨酸(0.37％),最大为谷氨酸(7.96％),贮藏时间对红松籽仁氨基酸含量有明显影响,大部分种类氨基酸的含量随贮藏时间增加而减少,红松种仁含氨基酸总量比苏联西伯利亚红松(P.sibirca)籽仁含氨基酸总量高7.64％。
