Introduction:Non-typhoidal Salmonella(NTS)represents a major global cause of foodborne illness.The emergence and worldwide dissemination of specific serotypes,including Salmonella London and Rissen,constitute a signif...Introduction:Non-typhoidal Salmonella(NTS)represents a major global cause of foodborne illness.The emergence and worldwide dissemination of specific serotypes,including Salmonella London and Rissen,constitute a significant public health threat due to their escalating association with antimicrobial resistance(AMR),which compromises the effectiveness of first-line antibiotic therapies.Methods:We performed a comprehensive genomic analysis integrating 200 local isolates collected between 2020 and 2024 from Shanghai,China,with a global dataset comprising 1,353 S.London and 882 S.Rissen genomes retrieved from EnteroBase.Through wholegenome sequencing,phylogenetic reconstruction,and AMR gene profiling,we systematically characterized the population structure,transmission dynamics,and resistance profiles of these serotypes.Results:Phylogeographic analysis revealed contrasting dissemination patterns:S.London spread predominantly through historical,geographically segregated clades,whereas S.Rissen demonstrated recent intercontinental mixing,with Thailand identified as a primary global source.We detected high-risk plasmids harboring up to 15 resistance genes that drove elevated multidrug resistance rates in 64%of S.London and 59%of S.Rissen isolates.Notably,Chinese isolates exhibited the highest AMR burden,with clinical environments identified as critical hotspots for resistance amplification.Conclusion:The global dissemination of S.London and Rissen is directly linked to international food trade networks,and their evolving AMR landscape represents a critical public health concern.These findings underscore the urgent need for integrated One Health surveillance strategies to effectively control the spread of resistant foodborne pathogens.展开更多
Influenza A virus has caused huge damage to human health and poultry production worldwide,but its global transmission patterns and influencing factors remain unclear.Here,by using the Nearest Genetic Distance Approach...Influenza A virus has caused huge damage to human health and poultry production worldwide,but its global transmission patterns and influencing factors remain unclear.Here,by using the Nearest Genetic Distance Approach with genetic sequences data,we reconstructed the global transmission patterns of 4 most common subtypes of influenza A virus(H1N1,H3N2,H5N1,and H7N9)and analyzed associations of transmission velocity of these influenza viruses with environmental factors.We found that the transmission patterns of influenza viruses and their associations with environmental factors were closely related to their host properties.H1N1 and H3N2,which are mainly held by humans,are transmitted between regions at high velocity and over long distances,which may be due to human transportation via airplane;while H5N1 and H7N9,which are mainly carried by animals,are transmitted locally at short distances and at low velocity,which may be facilitated by poultry transportation via railways or high ways.H1N1 and H3N2 spread faster in cold seasons,while H5N1 spread faster in both cold and warm seasons,and H7N9 spread faster in wet seasons.H1N1,H3N2,and H5N1 spread faster in places with both high and low human densities.Our study provided novel insights into the global transmission patterns,processes,and management strategies for influenza under accelerated global change.展开更多
基金Supported by the Research Project of Pudong New Area Health Commission(PW2023D-03)the Outstanding Leaders Training Program of Shanghai Pudong New Area Health Commission(PWRl2025-02)+1 种基金the Clinical Application and Resistance Evaluation of Antimicrobial Drugs by the National Health Commission(ECCUAEAR-2024-10)the National Natural Science Foundation of China(82530102,32370099).
文摘Introduction:Non-typhoidal Salmonella(NTS)represents a major global cause of foodborne illness.The emergence and worldwide dissemination of specific serotypes,including Salmonella London and Rissen,constitute a significant public health threat due to their escalating association with antimicrobial resistance(AMR),which compromises the effectiveness of first-line antibiotic therapies.Methods:We performed a comprehensive genomic analysis integrating 200 local isolates collected between 2020 and 2024 from Shanghai,China,with a global dataset comprising 1,353 S.London and 882 S.Rissen genomes retrieved from EnteroBase.Through wholegenome sequencing,phylogenetic reconstruction,and AMR gene profiling,we systematically characterized the population structure,transmission dynamics,and resistance profiles of these serotypes.Results:Phylogeographic analysis revealed contrasting dissemination patterns:S.London spread predominantly through historical,geographically segregated clades,whereas S.Rissen demonstrated recent intercontinental mixing,with Thailand identified as a primary global source.We detected high-risk plasmids harboring up to 15 resistance genes that drove elevated multidrug resistance rates in 64%of S.London and 59%of S.Rissen isolates.Notably,Chinese isolates exhibited the highest AMR burden,with clinical environments identified as critical hotspots for resistance amplification.Conclusion:The global dissemination of S.London and Rissen is directly linked to international food trade networks,and their evolving AMR landscape represents a critical public health concern.These findings underscore the urgent need for integrated One Health surveillance strategies to effectively control the spread of resistant foodborne pathogens.
基金This work was supported by grants from the National Natural Science Foundation of China(Grant Nos.3191101787,31970153,31630079)the National Key R&D Program of China(2016YFD0500206)+1 种基金the IUBS/ISZS program of“Biological Consequences of Global Change”,and Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29010000)J.L.is supported by Youth Innovation Promotion Association of CAS(2019091).
文摘Influenza A virus has caused huge damage to human health and poultry production worldwide,but its global transmission patterns and influencing factors remain unclear.Here,by using the Nearest Genetic Distance Approach with genetic sequences data,we reconstructed the global transmission patterns of 4 most common subtypes of influenza A virus(H1N1,H3N2,H5N1,and H7N9)and analyzed associations of transmission velocity of these influenza viruses with environmental factors.We found that the transmission patterns of influenza viruses and their associations with environmental factors were closely related to their host properties.H1N1 and H3N2,which are mainly held by humans,are transmitted between regions at high velocity and over long distances,which may be due to human transportation via airplane;while H5N1 and H7N9,which are mainly carried by animals,are transmitted locally at short distances and at low velocity,which may be facilitated by poultry transportation via railways or high ways.H1N1 and H3N2 spread faster in cold seasons,while H5N1 spread faster in both cold and warm seasons,and H7N9 spread faster in wet seasons.H1N1,H3N2,and H5N1 spread faster in places with both high and low human densities.Our study provided novel insights into the global transmission patterns,processes,and management strategies for influenza under accelerated global change.
