This study was aimed at evaluating the anti-diabetic potential of passion fruit Passiflora edulis(EPE) extracts in diabetic rats, following Streptozotocin(STZ) induced oxidative stress. Thirty adult Wistar rats were d...This study was aimed at evaluating the anti-diabetic potential of passion fruit Passiflora edulis(EPE) extracts in diabetic rats, following Streptozotocin(STZ) induced oxidative stress. Thirty adult Wistar rats were divided into five groups, with six rats in each group. The control rats were injected intraperitoneally with citrate buffer(pH 4.5). The remaining groups of rats were administered single dose of 45 mg·kg-1 of STZ by intraperitoneal route to induce diabetes. The diabetic animals were treated with 250 and 500 mg·kg-1 of EPE and glibenclamide 0.6 mg·kg-1 for fifteen days by oral route. Blood glucose, end organ oxidative stress marker, and anti-oxidants were assayed. Further, histopathological investigation of pancreas was studied at the end of the experimentation. The results revealed that subacute administration of EPE significantly(P < 0.001) controlled the blood glucose level in the diabetic rats. In addition, EPE extract protected the end organs by restoring the anti-oxidants enzyme, significantly increasing super oxide dismutase level(SOD) and decreasing catalase(CAT) and TBARS level in visceral organs. In conclusion, that EPE extracts showed anti-diabetic and anti-oxidant potential against streptozotocin-induced diabetes.展开更多
AIM： To investigate the effect of pomegranate juice（PJ） intake on overall oxidation status in retinas of diabetic rats.METHODS： Twenty-seven rats were divided into four groups as control（CO）, diabetic（DM）, con...AIM： To investigate the effect of pomegranate juice（PJ） intake on overall oxidation status in retinas of diabetic rats.METHODS： Twenty-seven rats were divided into four groups as control（CO）, diabetic（DM）, control treated with PJ（CO-PJ）, and diabetic treated with PJ（DM-PJ）.The retina tissues were used to determine 8-hydroxy-2＇-deoxyguanosine（8 OHd G）, malondialdehyde（MDA）, reduced glutathione（GSH） levels, and the enzyme activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）. RESULTS： The levels of 8 OHdG and MDA were significantly increased in the retina of DM group compared to CO group（P=0.001, P〈0.001 respectively）. Both 8 OHd G and MDA levels were decreased in PJ-DM group compared to DM group（P=0.004, P〈0.001 respectively）. The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group（P≤0.01）. GSH and GSH-Px activities were higher in PJ-DM group compared with DM group（P=0.010, P=0.042, respectively） but SOD activity was not statistically different（P=0.938）. CONCLUSION： PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.展开更多
文摘This study was aimed at evaluating the anti-diabetic potential of passion fruit Passiflora edulis(EPE) extracts in diabetic rats, following Streptozotocin(STZ) induced oxidative stress. Thirty adult Wistar rats were divided into five groups, with six rats in each group. The control rats were injected intraperitoneally with citrate buffer(pH 4.5). The remaining groups of rats were administered single dose of 45 mg·kg-1 of STZ by intraperitoneal route to induce diabetes. The diabetic animals were treated with 250 and 500 mg·kg-1 of EPE and glibenclamide 0.6 mg·kg-1 for fifteen days by oral route. Blood glucose, end organ oxidative stress marker, and anti-oxidants were assayed. Further, histopathological investigation of pancreas was studied at the end of the experimentation. The results revealed that subacute administration of EPE significantly(P < 0.001) controlled the blood glucose level in the diabetic rats. In addition, EPE extract protected the end organs by restoring the anti-oxidants enzyme, significantly increasing super oxide dismutase level(SOD) and decreasing catalase(CAT) and TBARS level in visceral organs. In conclusion, that EPE extracts showed anti-diabetic and anti-oxidant potential against streptozotocin-induced diabetes.
文摘AIM： To investigate the effect of pomegranate juice（PJ） intake on overall oxidation status in retinas of diabetic rats.METHODS： Twenty-seven rats were divided into four groups as control（CO）, diabetic（DM）, control treated with PJ（CO-PJ）, and diabetic treated with PJ（DM-PJ）.The retina tissues were used to determine 8-hydroxy-2＇-deoxyguanosine（8 OHd G）, malondialdehyde（MDA）, reduced glutathione（GSH） levels, and the enzyme activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）. RESULTS： The levels of 8 OHdG and MDA were significantly increased in the retina of DM group compared to CO group（P=0.001, P〈0.001 respectively）. Both 8 OHd G and MDA levels were decreased in PJ-DM group compared to DM group（P=0.004, P〈0.001 respectively）. The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group（P≤0.01）. GSH and GSH-Px activities were higher in PJ-DM group compared with DM group（P=0.010, P=0.042, respectively） but SOD activity was not statistically different（P=0.938）. CONCLUSION： PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.
