Fatty acids are the main constituents of vegetable oils.To determine the fatty acid compositions of small trade vegetable oils and some less well studied beneficial vegetable oils,and investigate their relationships w...Fatty acids are the main constituents of vegetable oils.To determine the fatty acid compositions of small trade vegetable oils and some less well studied beneficial vegetable oils,and investigate their relationships with antioxidant activity and oxidative stability,gas chromatography-mass spectrometry was performed to characterize the associated fatty acid profiles.The antioxidant activity of vegetable oils,based on their DPPH-scavenging capacity(expressed as IC_(50) values),was used to assess their impact on human health,and their oxidative stability was characterized by performing lipid oxidation analysis to determine the oxidative induction time of fats and oils.In addition,correlation analyses were performed to examine associations between the fatty acid composition of the oils and DPPH-scavenging capacity and oxidative stability.The results revealed that among the assessed oils,coffee seed oil has the highest saturated fatty acid content(355.10 mg/g),whereas Garddenia jaminoides oil has the highest unsaturated fatty acid content(844.84 mg/g).Coffee seed oil was also found have the lowest DPPH IC_(50) value(2.30 mg/mL)and the longest oxidation induction time(17.09 h).Correlation analysis revealed a significant linear relationship(P<0.05)between oxidative stability and unsaturated fatty acid content,with lower contents tending to be associated with better oxidative stability.The findings of this study provide reference data for the screening of functional edible vegetable oils.展开更多
Heat stress hinders the growth and productivity of sweetpotato plants,predominantly through oxidative damage to cellular membranes.Therefore,the development of efficient approaches for mitigating heat-related impairme...Heat stress hinders the growth and productivity of sweetpotato plants,predominantly through oxidative damage to cellular membranes.Therefore,the development of efficient approaches for mitigating heat-related impairments is essential for the long-term production of sweetpotatoes.Melatonin has been recognised for its capacity to assist plants in dealing with abiotic stress conditions.This research aimed to investigate how different doses of exogenous melatonin influence heat damage in sweetpotato plants.Heat stress drastically affected shoot and root fresh weight by 31.8 and 44.5%,respectively.This reduction resulted in oxidative stress characterised by increased formation of hydrogen peroxide(H_(2)O_(2))by 804.4%,superoxide ion(O_(2)^(·-))by 211.5%and malondialdehyde(MDA)by 234.2%.Heat stress also reduced chlorophyll concentration,photosystemⅡefficiency(F_v/F_m)by 15.3%and gaseous exchange.However,pre-treatment with 100μmol L^(-1)melatonin increased growth and reduced oxidative damage to sweetpotato plants under heat stress.In particular,melatonin decreased H_(2)O_(2),O_(2)^(·-)and MDA by 64.8%,42.7%and 38.2%,respectively.Melatonin also mitigated the decline in chlorophyll levels and improved stomatal traits,gaseous exchange and F_(v)/F_(m)(13%).Results suggested that the favorable outcomes of melatonin treatment can be associated with elevated antioxidant enzyme activity and an increase in non-enzymatic antioxidants and osmo-protectants.Overall,these findings indicate that exogenous melatonin can improve heat stress tolerance in sweetpotatoes.This stu dy will assist re searchers in further investigating how melatonin makes sweetpotatoes more resistant to heat stress.展开更多
Gastric cancer is one of common malignant tumors from a global perspective, and its morbidity ranks the forth and also the second largest cause of cancer-related death worldwide. Many factors can cause gastric cancer,...Gastric cancer is one of common malignant tumors from a global perspective, and its morbidity ranks the forth and also the second largest cause of cancer-related death worldwide. Many factors can cause gastric cancer, including helicobacter pylori infection, chronic inflammation, genetic factors et al. Among all of these, helicobacter pylori infection can significantly increase the production of reactive oxygen species（ROS） and reactive nitrogen species（RNS） in human stomach, which can cause the oxidative stress. Oxidative stress plays an important role in the pathogenesis of gastro-intestinal diseases such as mucosal damage, gastro-intestinal ulcers and cancer. Modern therapeutic treatments such as surgery and chemotherapy have undesired side effects, so the antioxidant therapy gains more and more attentions. Antioxidant therapy system comprises of various antioxidants（SOD, catalase, glutathione peroxidase and carnosine） and Chinese herbal medicine, which is mainly focused on the chemoprevention. Natural products and their derivatives, such as tea polyphenol, resveratrol and vitamins, have some potential benefits on their chemoprevention. Besides, much work has been done to understand the role of dietary factors playing in the prevention of gastrointestinal cancers. In this review based on some valuable studies, we aim to make some brief summaries about risk factors, pathogenic mechanism of oxidative stress and antioxidants therapy in gastric cancer.展开更多
BACKGROUND Acrylamide(ACR),a toxic compound commonly found in heat-processed foods,poses a serious risk to liver health due to its oxidative and inflammatory effects.AIM To evaluate the hepatoprotective potential of g...BACKGROUND Acrylamide(ACR),a toxic compound commonly found in heat-processed foods,poses a serious risk to liver health due to its oxidative and inflammatory effects.AIM To evaluate the hepatoprotective potential of ginger extract in mitigating ACR-induced liver toxicity in a rat model.METHODS Male Sprague-Dawley rats were randomly assigned into control,ACR-treated,and ACR+ginger-treated groups.Liver function enzymes[alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)],oxidative stress biomarkers[malondialdehyde(MDA),glutathione(GSH),catalase(CAT),superoxide dismutase(SOD)],and histopathological assessments were performed.In addition,gene expression analyses of key antioxidant and inflammatory markers were conducted using quantitative polymerase chain reaction.RESULTS ACR administration significantly increased serum levels of ALT,AST,ALP,and MDA,while reducing levels of GSH,CAT,and SOD.Histological analysis revealed hepatic degeneration and inflammation.Co-administration of ginger extract significantly reversed these effects,restoring antioxidant enzyme levels,reducing oxidative stress,and improving liver histoarchitecture.CONCLUSION Ginger extract exhibited strong hepatoprotective effects against ACR-induced toxicity through antioxidant and anti-inflammatory mechanisms.These findings support the potential role of ginger as a natural dietary intervention for mitigating liver damage caused by environmental toxins.Further clinical studies are recommended to confirm its efficacy in human populations.展开更多
Oocyte cryopreservation is an essential procedure in assisted reproductive technologies,aimed at preserving fertility,particularly for women undergoing IVF treatment or at risk of ovarian damage due to radiation,chemo...Oocyte cryopreservation is an essential procedure in assisted reproductive technologies,aimed at preserving fertility,particularly for women undergoing IVF treatment or at risk of ovarian damage due to radiation,chemotherapy,or surgery.Despite its growing use,the survival and fertilization rates of cryopreserved oocytes remain suboptimal,largely due to cryo-induced oxidative stress.The generation of Reactive Oxygen Species(ROS)during freezing and thawing causes considerable damage to key cellular components,including proteins,lipids,DNA,and mitochondria.This oxidative stress compromises oocyte quality and reduces developmental potential.To address these challenges,the use of additives-especially antioxidants-has shown significant promise in mitigating oxidative damage.Enzymatic antioxidants such as Superoxide Dismutase(SOD)and Catalase(CAT),along with non-enzymatic antioxidants like glutathione,melatonin,and resveratrol,have demonstrated the ability to neutralize ROS and improve oocyte viability and developmental outcomes.Recent studies highlight the potential of Mitoquinone(MitoQ),a mitochondria-targeted antioxidant,to effectively counteract mitochondrial ROS and enhance cellular defense mechanisms during cryopreservation.This review explores the cellular mechanisms of cryodamage,the role of oxidative stress in oocyte cryopreservation,and the potential of various antioxidant strategies to enhance oocyte survival and function.Developing effective antioxidant supplementation approaches may significantly improve the outcomes of cryopreservation in reproductive medicine.展开更多
Artemisia dracunculus L.,or tarragon,is a perennial herb from the Asteraceae family that is extensively cultivated for its aromatic leaves,which are valued for its preventative and therapeutic properties in both cooke...Artemisia dracunculus L.,or tarragon,is a perennial herb from the Asteraceae family that is extensively cultivated for its aromatic leaves,which are valued for its preventative and therapeutic properties in both cookery and traditional medicine.This study aims to investigate the antibacterial,antioxidant,and anti-inflammatory properties of A.dracunculus(tarragon)essential oil(ADEO),with estragole(57.23%)identified as the major compound through gas chromatography-mass spectrometry(GC-MS)analysis.ADEO exhibited varying degrees of antibacterial activity,with Escherichia coli showing higher resistance inhibition zone(IZ)=14.7±0.58 mm,minimum (inhibitory concentration(MIC)=2%and minimum bactericidal concentration(MBC)=4%),while Bacillus subtilis(IZ=24.05±2.11 mm and MIC=MBC=0.125%)and Staphylococcus aureus(IZ=18.69±1.45 mm,MIC=0.0612%and MBC=0.125%)were more sensitive to its actions.Antioxidant ability was assessed using 1,1-diphenyl-2-picrylhydrazil(DPPH),2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),xanthine oxidase inhibition,and beta-carotene bleaching assays.ADEO showed remarkable antiradical effect on DPPH(IC_(50)=127.05±3.47μg/mL)and ABTS radical(IC_(50)=89.60±8.73μg/mL)as well as significant inhibition of xanthine oxidase(IC_(50)=47.9±2.04μg/mL)and lipid peroxidation(IC_(50)=231.63±5.21μg/mL).ADEO also showed significant anti-inflammatory activity by inhibiting the enzymesCOX-1(IC_(50)=59.2±2.43μg/mL),Cyclo-oxygenase-2(COX-2)(IC_(50)=74.68±1.34μg/mL)and 5-lipooxygense(5-LOX)(IC_(50)=93.18±1.87μg/mL),which are involved in the inflammatory pathway.These findings suggest that ADEO,with its high estragole content,holds promising potential as a natural antibacterial,antioxidant,and anti-inflammatory agent for preventive and therapeutic applications.Further research is needed to explore its safety and efficacy in clinical settings.展开更多
Identification of natural substances with antioxidant properties is ongoing research for addressing issues related to oxidative stress especially attributed to environmental effects.Our previous study demonstrated tha...Identification of natural substances with antioxidant properties is ongoing research for addressing issues related to oxidative stress especially attributed to environmental effects.Our previous study demonstrated that Lateolabrax japonicus peptides(LPH),rich in Glu,Gly,and hydrophobic amino acids,exhibited remarkable antioxidant activity in vitro,with though its action mechanism yet to be revealed.Therefore,to assess the in vivo antioxidative properties of LPH,we employed H_(2)O_(2) to generate oxidative stress in Drosophila melanogaster model.Results indicated that LPH significantly prolonged the lifespan of Drosophila subjected to oxidative stress mostly mediated via LPH’s enhancement of the antioxidant defense system and intestinal functions.Antioxidant effects were manifested by a decrease in malondialdehyde(MDA)levels,elevated superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities,decreased levels of reactive oxygen species(ROS)in intestinal epithelial cells,and the preservation of intestinal length.LPH effectively controlled the excessive proliferation and differentiation of oxidative stress-induced Drosophila intestinal stem cells.At the gene level,LPH upregulated the expression of antioxidant-related Nrf2 genes while concurrently downregulated mTOR expression level.Furthermore,high-throughput 16S rDNA sequencing revealed that the addition of LPH significantly influenced the diversity and abundance of the intestinal microbiota in H_(2)O_(2)-induced Drosophila.These findings provide a deeper understanding of the antioxidative mechanism of LPH,suggesting its potential applications in food industry and to be assessed using other in vivo oxidative stress models.展开更多
Oxidative stress,characterized by the excessive accumulation of reactive oxygen species(ROS),is linked to various pathological conditions,including myocardial infarction,cancer,and neurodegenerative diseases.Addressin...Oxidative stress,characterized by the excessive accumulation of reactive oxygen species(ROS),is linked to various pathological conditions,including myocardial infarction,cancer,and neurodegenerative diseases.Addressing ROS-induced cell damage has become a critical focus of biomedical research.In this study,a thermo-sensitive poly(amino acid)hydrogel,composed of poly(ethylene glycol)-block-poly(l-methionine),was prepared for cytoprotection through ROS scavenging.The sol-to-gel transition mechanism of the hydrogel was elucidated,and its potent antioxidant properties and cell protective effects were validated using hydrogen peroxide(H_(2)O_(2))-induced oxidative stress and oxygen-glucose deprivation(OGD)models.The hydrogel significantly mitigated H_(2)O_(2)-induced damage in L929 cells,doubling their survival rate.Additionally,it scavenged approximately 35.8%of the ROS during OGD,reducing mitochondrial oxidative damage and resulting in a 29.4%decrease in apoptotic cell number.These findings underscore the potential biomedical applications of thermo-sensitive poly(amino acid)hydrogels,particularly in treating oxidative stress-related cell damage.展开更多
High-efficient rubber antioxidants for enhanced heat resistance without compromising mechanical properties remain an enormous and long-term challenge for the rubber industry.Herein,we employed the in-situ growth of Ce...High-efficient rubber antioxidants for enhanced heat resistance without compromising mechanical properties remain an enormous and long-term challenge for the rubber industry.Herein,we employed the in-situ growth of Ce-doped Co-metal-organic framework(Ce Co-MOF)in dendritic mesoporous organosilica nanoparticles(DMONs@Ce Co-MOF,denoted as DCCM)to prepare a novel antioxidant that exhibit outstanding thermal stability.Dendritic mesoporous organosilica nanoparticles(DMONs)effectively alleviated the incompatibility of Ce Co-MOF in the polymer matrix,and the effective scavenging of free radicals was attributed to the various oxidation states of metal ions in Ce Co-MOF.Surprising,by adding only0.5 phr(parts per hundred of rubber)of DMONs@Ce Co-MOF to silicone rubber,(SR),the retention rate of tensile strength increased from 37.3%to 61.6%after aging 72 h at 250℃,and the retention rate of elongation at break of DCCM/SR1 composites reached 68%,which was 5.43 times of SR.The strategy of anchoring MOFs on the surface of silica also provides a viable method for preparing effective compound functionalized rubber antioxidant.展开更多
[Objective] The aim of the study was to determine whether phytotoxicity of TeA against Echinochloa crus-galli leaves was correlated with oxidative stress caused by generation of reactive oxygen and the changes of anti...[Objective] The aim of the study was to determine whether phytotoxicity of TeA against Echinochloa crus-galli leaves was correlated with oxidative stress caused by generation of reactive oxygen and the changes of antioxidant enzymes activity.[Method] The changes of malondialdehyde（MDA）content,hydrogen peroxide（H2O2）,and activities of superoxide dismutase（SOD）,glutathione reductase（GR）and catalase（CAT）were studied by leaf segment method in vitro.[Result] After the treatment of 500 μmol/L TeA,the content of MDA and H2O2 increased by 247.86% and 67.00%,respectively,indicating that the accumulation of MDA and H2O2 in E.crus-galli leaves was due to the reactive oxygen burst induced by TeA.TeA induced a significant increase in activities of SOD,GR and CAT.At 500 μmol/L TeA,activities of SOD,GR and CAT increased more than one fold compared with the control.[Conclusion] TeA could not only cause oxidative stress in leaves of E.crus-galli through the induction of reactive oxygen,but also induce the increasing of antioxidant enzyme activity.展开更多
Solid oxide fuel cell(SOFC)is a promising energy conversion device because of its high efficiency and fuel/oxidant flexibility,enabling the direct conversion of chemical energy to electrical power.By using N_(2)O as a...Solid oxide fuel cell(SOFC)is a promising energy conversion device because of its high efficiency and fuel/oxidant flexibility,enabling the direct conversion of chemical energy to electrical power.By using N_(2)O as an oxidant instead of air,we develop a dual-phase catalyst La_(0.6)Sr_(0.4)Co_(0.2)Fe_(0.8)O_(3-δ)-Gd_(0.1)Ce_(0.9)O_(2-δ)(LSCF-GDC),which exhibits efficient bifunctionality as a cathode for SOFC,synergistic ally promoting simultaneous N_(2)O decomposition and oxygen reduction kinetics.展开更多
The article by Mansouri et al provides a comprehensive investigation into the effects of L-arginine(L-Arg)on diabetic cardiomyopathy.The authors conclude that while a low dose(0.5 g/kg)of L-Arg improves lipid profiles...The article by Mansouri et al provides a comprehensive investigation into the effects of L-arginine(L-Arg)on diabetic cardiomyopathy.The authors conclude that while a low dose(0.5 g/kg)of L-Arg improves lipid profiles and reduces body weight,higher doses(≥1 g/kg)exacerbate oxidative stress,inflammation,and myocardial damage.In this letter,we aim to expand on the potential role of anti-inflammatory and antioxidant strategies in mitigating these adverse effects.Specifically,we focus on nuclear factor erythroid 2-related factor 2 activation and nitric oxide synthase modulation.These strategies could enhance the clinical utility of L-Arg by preserving its metabolic benefits while reducing its cardiotoxic risks.We believe this perspective will stimulate future research on L-Arg-based therapies in patients with diabetes,with an emphasis on optimizing dosage and exploring synergistic co-therapies.展开更多
Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group...Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS.Results Implementing cyclic chronic HS(34 ℃ for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the m RNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis.Conclusions These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers.展开更多
Background:Ischemic stroke is a disease characterized by the damage of brain tissue due to insufficient blood supply.The neuronal necrosis caused by oxidative stress during the acute phase of ischemic stroke leads to ...Background:Ischemic stroke is a disease characterized by the damage of brain tissue due to insufficient blood supply.The neuronal necrosis caused by oxidative stress during the acute phase of ischemic stroke leads to serious consequences,including blood-brain barrier disruption and vascular aging.The Kelch-like ECH-associated protein 1(KEAP1),is a key switch of antioxidative system in human body.Until now,there is still a lack of effective treatment to ischemic stroke.Methods:We developed scutellarin-based liposomes for treating ischemic stroke injury caused neuronal damage.Results:The results showed that scutellarin could directly bind to KEAP1 protein,and the Kd was 26.1μM.The scutellarin-based liposomes significantly reduced cellular reactive oxygen species(ROS)levels.It could also upregulate the protein expression level of nuclear factor E2-related factor 2(NRF2),which is the substrate protein of KEAP1.Next,both the mRNA and protein expression level of the NRF2 downstream anti-oxidative element,heme oxygenase 1(HO-1)and NAD(P)H quinone dehydrogenase 1(NQO1)were promoted.Furthermore,the coimmunoprecipitation(Co-IP)and hydrogen-deuterium exchange mass spectrometry(HDX-MS)revealed that scutellarin directly bound to KEAP1’s Kelch domain,interrupting the interaction between KEAP1 and NRF2.Conclusion:Our work indicates that the scutellarin-based liposomes might be a promising therapeutic approach for ischemic stroke induced neuronal necrosis.展开更多
Thymus serpyllum L.,often known as wild thyme,has been used since ancient times due to its multifaceted culinary and medicinal attributes.It is usually utilized in folk medicine to manage different health issues.This ...Thymus serpyllum L.,often known as wild thyme,has been used since ancient times due to its multifaceted culinary and medicinal attributes.It is usually utilized in folk medicine to manage different health issues.This work aimed to investigate the chemical composition and biological characteristics of T.serpyllum essential oil(EO),including its antimicrobial,antioxidant,and anti-inflammatory capabilities.Moreover,we have prompted an in-silico simulation to reveal the underlying mode of action of these properties.The chemical characterization of T.serpyllum(EO)by Gas Chromatography-Mass Spectrometry(GC-MS)indicated sabinene(17.33%),terpinen-4-ol(11.73%),phellandral(13.18%),and thymol(10.54%)as main components.The antimicrobial screening utilized the disc-diffusion technique,MIC,and MBC assays.The disc-diffusion test’s results revealed significant anti-Candida activity and notable antibacterial efficacy.The MIC and MBC tests showed that T.serpyllum EO effectively stops bacterial growth,including Gram-positive and Gram-negative strains and Candida strains.The tolerance level ratio demonstrated that this EO exhibits bactericidal and fungicidal effects on all tested bacteria and Candida strains.Also,T.serpyllum EO presented effective inhibitory activity against the 5-lipoxygenase(5-LOX)enzyme(IC50=744.19±0.1µg/mL)(p<0.05).It also effectively affected FRAP,β-carotene,DPPH,and ABTS radicals.In light of these findings,T.serpyllum holds promise for diverse applications across pharmaceuticals,nutraceuticals,and the food industry.However,further research and collaboration between traditional knowledge and modern medicine are crucial to fully realizing its potential benefits in these fields.展开更多
Nickel(Ni)toxicity significantly impairs plant growth,photosynthesis,and metabolism by inducing oxidative stress.This study evaluates the potential of exogenous Alpha-Ketoglutarate(AKG)in mitigating Ni-induced stress ...Nickel(Ni)toxicity significantly impairs plant growth,photosynthesis,and metabolism by inducing oxidative stress.This study evaluates the potential of exogenous Alpha-Ketoglutarate(AKG)in mitigating Ni-induced stress in Peganum harmala L.Seedlings were exposed to 0,200,500,and 750μM NiCl2,with or without AKG supplementation.Under 750μM Ni stress,dry weight(DW)decreased by 33.7%,tissue water content(TWC)by 39.9%,and chlorophyll a and total chlorophyll levels were reduced by 17%and 15%,respectively.Ni exposure also significantly increased secondary metabolite production,with leaf anthocyanin content rising by 131%,and superoxide dismutase(SOD)and catalase(CAT)activities increasing by 228%and 53%,respectively,in roots at 500μM Ni.AKG treatment alleviated Ni toxicity by enhancing TWC by 39%and promoting root and shoot growth.Additionally,AKG treatment boosted the synthesis of phenolic compounds and flavonoids,contributing to improved tolerance against Ni stress.These findings demonstrate the potential of AKG in enhancing Ni tolerance in P.harmala,suggesting its promising role in bioremediation of metal-contaminated soils.This is the first study to report the beneficial effects of exogenous AKG in alleviating nickel toxicity in P.harmala L.,offering a new approach for improving plant resilience to heavy metal stress.展开更多
Arsenic(As)and chromium(Cr)are two harmful toxicants as well as carcinogens which can coexist in polluted surface water and groundwater.This coexistence leads to mixture effects in animals including fish.Both of these...Arsenic(As)and chromium(Cr)are two harmful toxicants as well as carcinogens which can coexist in polluted surface water and groundwater.This coexistence leads to mixture effects in animals including fish.Both of these heavy metals are reported to manifest reactive oxygen species(ROS)mediated toxicity.Though individual neurotoxic effects have been reported,their mixture effects,its mechanism and cellular responses against oxidative stress and DNA damages remain unknown.The present study evaluated the individual and mixture effects of As and Cr at their environmentally relevant concentrations in zebrafish(Danio rerio)brain after 15,30 and 60 days of exposure.Nrf2,a transcription factor is involved in the expressional regulation of enzymes needed to maintain cellular redox homeostasis.This study reported the expressional pattern of Nrf2 and its associated xenobiotic metabolizing enzyme Nqo1 and other markers of oxidative stress such as ROS generation,reduced glutathione level,lipid peroxidation and catalase activity.Increased malondialdehyde(MDA)content,glutathione level,and catalase activity indicated oxidative stress in exposed groups.In addition,this study revealed expressional alterations of neurotoxicity marker(ache),DNA repair(ogg1,apex1,creb1,polb,mlh1,msh2 and msh6)and tumor suppressor(p53,brca2)genes.Results of ROS generation,MDA level,histopathological analysis,gene expression and immunofluorescence study confirmed that As and Cr did not show antagonistic effects in combination rather indicated additive effects which was dose-dependent but not always linear.展开更多
Oxidative stress is a critical risk factor for various disease complications in patients with end-stage renal disease,which may be further aggravated during hemodialysis.Herein,we prepared a hemodialysis membrane by i...Oxidative stress is a critical risk factor for various disease complications in patients with end-stage renal disease,which may be further aggravated during hemodialysis.Herein,we prepared a hemodialysis membrane by introducing poly(2-Acrylamide-2-methylpropanesulfonic acid-N-vinylpyrrolidone)(P(AMPS-VP))into the polyethersulfone(PES)membrane by simple in-situ polymerization and non-solvent phase sepa-ration(NIPS)method,followed by tannic acid(TA)coating construction through hydrogen bonding interaction,termed PES/P(AMPS-VP)-TA.The membrane can efficiently remove reactive nitrogen radicals(RNS)(DPPH•,89.96%;ABTS•+,90.49%)and reactive oxygen species(ROS)(O_(2)•^(−),90.45%),and has a very sta-ble antioxidant property.Meanwhile,the membrane does not cause hemolysis and coagulation,and has superior blood compatibility required by hemodialysis membrane.In addition,the dialysis performance of the membrane is improved compared with unmodified PES,which is beneficial for practical hemodialysis applications.This work provides a reference for industrial preparation of hemodialysis membranes,which is expected to be applied to reduce oxidative stress in hemodialysis patients.展开更多
Background:To explore the antioxidant capacity ofα-asarone(ARE)in mice models of sodium valproate(SV)-induced liver injury,and to elucidate the underlying mechanism of ARE in liver injury treatment.Methods:Network ph...Background:To explore the antioxidant capacity ofα-asarone(ARE)in mice models of sodium valproate(SV)-induced liver injury,and to elucidate the underlying mechanism of ARE in liver injury treatment.Methods:Network pharmacology and molecular docking were used to predict ARE’s potential pharmacological mechanisms in treating drug-induced liver injury.AML12 cell model was used to evaluate the antioxidant stress activation of ARE.In the evaluation of antioxidant activity,ARE was tested for its ability to scavenge hydroxyl radical(OH)radicals,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+),and 1,1-diphenyl-2-picrylhydrazyl(DPPH),with vitamin C serving as the positive control.And thirty-six male mice were divided into six groups.Group I,the control,received saline.The other five groups received oral doses of 500 mg/kg/b.w.of SV daily for 14 days.Group II received only SV.Group III received the hepatic protectant bifendate at 100 mg/kg/b.w.,while groups IV,V,and VI were treated with 20,40,and 80 mg/kg/b.w.of ARE,respectively,also for 14 days.After sacrifice,liver and blood samples were collected for biochemical or metabolomic analysis.Results:ARE significantly reduced the levels of reactive oxygen species and activated the Keap1/Nrf2 signaling pathway in liver injury AML12 cells.ARE demonstrated dose-dependent scavenging activity in DPPH,ABTS+,and OH assays.It lowered serum alanine aminotransferase and aspartate aminotransferase levels,increased glutathione peroxidase and superoxide dismutase activity in liver tissue,and reduced malondialdehyde content.Metabolomic analysis showed that ARE restored twenty liver metabolites to control levels and enriched three pathways(arginine biosynthesis,sucrose and starch metabolism,and biotin metabolism)with shared differential metabolites.Conclusion:These results shed light on ARE’s mechanism in reducing oxidative stress,suggesting a potential strategy for preventing SV-induced liver disease by modulating metabolic products and enhancing antioxidant capacity.ARE could be a promising drug candidate for its antioxidant and liver-protective effects.展开更多
This study explores the unique role of CO_(2)as an oxidant in stainless steel smelting,focusing on its effectiveness in decarbonization and chromium retention.The research begins by theoretically demonstrating that al...This study explores the unique role of CO_(2)as an oxidant in stainless steel smelting,focusing on its effectiveness in decarbonization and chromium retention.The research begins by theoretically demonstrating that although the introduction of CO_(2)increases the CO partial pressure in the reaction system,the decarburization and chromium(Cr)retention capabilities of CO_(2)can still be stably maintained through the rational adjustment of the molten steel composition,temperature,and inert gas proportions.Further experimental findings indicate that chromium does not exhibit significant oxidation losses when the carbon(C)content exceeds 1.0%(mass).Finally,a novel CO_(2)recovery and utilization approach is proposed,integrating CO_(2)capture from smelting flue gas and recycling it for smelting,reducing O_(2)consumption and energy costs.This innovative process,compatible with existing smelting plants,presents a promising pathway towards carbon neutrality in the iron and steel industry,bridging theoretical insights with practical applications.展开更多
文摘Fatty acids are the main constituents of vegetable oils.To determine the fatty acid compositions of small trade vegetable oils and some less well studied beneficial vegetable oils,and investigate their relationships with antioxidant activity and oxidative stability,gas chromatography-mass spectrometry was performed to characterize the associated fatty acid profiles.The antioxidant activity of vegetable oils,based on their DPPH-scavenging capacity(expressed as IC_(50) values),was used to assess their impact on human health,and their oxidative stability was characterized by performing lipid oxidation analysis to determine the oxidative induction time of fats and oils.In addition,correlation analyses were performed to examine associations between the fatty acid composition of the oils and DPPH-scavenging capacity and oxidative stability.The results revealed that among the assessed oils,coffee seed oil has the highest saturated fatty acid content(355.10 mg/g),whereas Garddenia jaminoides oil has the highest unsaturated fatty acid content(844.84 mg/g).Coffee seed oil was also found have the lowest DPPH IC_(50) value(2.30 mg/mL)and the longest oxidation induction time(17.09 h).Correlation analysis revealed a significant linear relationship(P<0.05)between oxidative stability and unsaturated fatty acid content,with lower contents tending to be associated with better oxidative stability.The findings of this study provide reference data for the screening of functional edible vegetable oils.
基金supported jointly by the earmarked fund for CARS-10-GW2the key research and development program of Hainan Province(Grant No.ZDYF2020226)+1 种基金Collaborative innovation center of Nanfan and high-efficiency tropical agriculture,Hainan University(Grant No.XTCX2022NYC21)funding of Hainan University[Grant No.KYQD(ZR)22123]。
文摘Heat stress hinders the growth and productivity of sweetpotato plants,predominantly through oxidative damage to cellular membranes.Therefore,the development of efficient approaches for mitigating heat-related impairments is essential for the long-term production of sweetpotatoes.Melatonin has been recognised for its capacity to assist plants in dealing with abiotic stress conditions.This research aimed to investigate how different doses of exogenous melatonin influence heat damage in sweetpotato plants.Heat stress drastically affected shoot and root fresh weight by 31.8 and 44.5%,respectively.This reduction resulted in oxidative stress characterised by increased formation of hydrogen peroxide(H_(2)O_(2))by 804.4%,superoxide ion(O_(2)^(·-))by 211.5%and malondialdehyde(MDA)by 234.2%.Heat stress also reduced chlorophyll concentration,photosystemⅡefficiency(F_v/F_m)by 15.3%and gaseous exchange.However,pre-treatment with 100μmol L^(-1)melatonin increased growth and reduced oxidative damage to sweetpotato plants under heat stress.In particular,melatonin decreased H_(2)O_(2),O_(2)^(·-)and MDA by 64.8%,42.7%and 38.2%,respectively.Melatonin also mitigated the decline in chlorophyll levels and improved stomatal traits,gaseous exchange and F_(v)/F_(m)(13%).Results suggested that the favorable outcomes of melatonin treatment can be associated with elevated antioxidant enzyme activity and an increase in non-enzymatic antioxidants and osmo-protectants.Overall,these findings indicate that exogenous melatonin can improve heat stress tolerance in sweetpotatoes.This stu dy will assist re searchers in further investigating how melatonin makes sweetpotatoes more resistant to heat stress.
基金National Natural Science Foundation of China(Grant Nos.31471338,81260338)
文摘Gastric cancer is one of common malignant tumors from a global perspective, and its morbidity ranks the forth and also the second largest cause of cancer-related death worldwide. Many factors can cause gastric cancer, including helicobacter pylori infection, chronic inflammation, genetic factors et al. Among all of these, helicobacter pylori infection can significantly increase the production of reactive oxygen species（ROS） and reactive nitrogen species（RNS） in human stomach, which can cause the oxidative stress. Oxidative stress plays an important role in the pathogenesis of gastro-intestinal diseases such as mucosal damage, gastro-intestinal ulcers and cancer. Modern therapeutic treatments such as surgery and chemotherapy have undesired side effects, so the antioxidant therapy gains more and more attentions. Antioxidant therapy system comprises of various antioxidants（SOD, catalase, glutathione peroxidase and carnosine） and Chinese herbal medicine, which is mainly focused on the chemoprevention. Natural products and their derivatives, such as tea polyphenol, resveratrol and vitamins, have some potential benefits on their chemoprevention. Besides, much work has been done to understand the role of dietary factors playing in the prevention of gastrointestinal cancers. In this review based on some valuable studies, we aim to make some brief summaries about risk factors, pathogenic mechanism of oxidative stress and antioxidants therapy in gastric cancer.
文摘BACKGROUND Acrylamide(ACR),a toxic compound commonly found in heat-processed foods,poses a serious risk to liver health due to its oxidative and inflammatory effects.AIM To evaluate the hepatoprotective potential of ginger extract in mitigating ACR-induced liver toxicity in a rat model.METHODS Male Sprague-Dawley rats were randomly assigned into control,ACR-treated,and ACR+ginger-treated groups.Liver function enzymes[alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)],oxidative stress biomarkers[malondialdehyde(MDA),glutathione(GSH),catalase(CAT),superoxide dismutase(SOD)],and histopathological assessments were performed.In addition,gene expression analyses of key antioxidant and inflammatory markers were conducted using quantitative polymerase chain reaction.RESULTS ACR administration significantly increased serum levels of ALT,AST,ALP,and MDA,while reducing levels of GSH,CAT,and SOD.Histological analysis revealed hepatic degeneration and inflammation.Co-administration of ginger extract significantly reversed these effects,restoring antioxidant enzyme levels,reducing oxidative stress,and improving liver histoarchitecture.CONCLUSION Ginger extract exhibited strong hepatoprotective effects against ACR-induced toxicity through antioxidant and anti-inflammatory mechanisms.These findings support the potential role of ginger as a natural dietary intervention for mitigating liver damage caused by environmental toxins.Further clinical studies are recommended to confirm its efficacy in human populations.
基金Anhui Province Clinical Medical Research Translation Special Program(No.2204295107020002).
文摘Oocyte cryopreservation is an essential procedure in assisted reproductive technologies,aimed at preserving fertility,particularly for women undergoing IVF treatment or at risk of ovarian damage due to radiation,chemotherapy,or surgery.Despite its growing use,the survival and fertilization rates of cryopreserved oocytes remain suboptimal,largely due to cryo-induced oxidative stress.The generation of Reactive Oxygen Species(ROS)during freezing and thawing causes considerable damage to key cellular components,including proteins,lipids,DNA,and mitochondria.This oxidative stress compromises oocyte quality and reduces developmental potential.To address these challenges,the use of additives-especially antioxidants-has shown significant promise in mitigating oxidative damage.Enzymatic antioxidants such as Superoxide Dismutase(SOD)and Catalase(CAT),along with non-enzymatic antioxidants like glutathione,melatonin,and resveratrol,have demonstrated the ability to neutralize ROS and improve oocyte viability and developmental outcomes.Recent studies highlight the potential of Mitoquinone(MitoQ),a mitochondria-targeted antioxidant,to effectively counteract mitochondrial ROS and enhance cellular defense mechanisms during cryopreservation.This review explores the cellular mechanisms of cryodamage,the role of oxidative stress in oocyte cryopreservation,and the potential of various antioxidant strategies to enhance oocyte survival and function.Developing effective antioxidant supplementation approaches may significantly improve the outcomes of cryopreservation in reproductive medicine.
文摘Artemisia dracunculus L.,or tarragon,is a perennial herb from the Asteraceae family that is extensively cultivated for its aromatic leaves,which are valued for its preventative and therapeutic properties in both cookery and traditional medicine.This study aims to investigate the antibacterial,antioxidant,and anti-inflammatory properties of A.dracunculus(tarragon)essential oil(ADEO),with estragole(57.23%)identified as the major compound through gas chromatography-mass spectrometry(GC-MS)analysis.ADEO exhibited varying degrees of antibacterial activity,with Escherichia coli showing higher resistance inhibition zone(IZ)=14.7±0.58 mm,minimum (inhibitory concentration(MIC)=2%and minimum bactericidal concentration(MBC)=4%),while Bacillus subtilis(IZ=24.05±2.11 mm and MIC=MBC=0.125%)and Staphylococcus aureus(IZ=18.69±1.45 mm,MIC=0.0612%and MBC=0.125%)were more sensitive to its actions.Antioxidant ability was assessed using 1,1-diphenyl-2-picrylhydrazil(DPPH),2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),xanthine oxidase inhibition,and beta-carotene bleaching assays.ADEO showed remarkable antiradical effect on DPPH(IC_(50)=127.05±3.47μg/mL)and ABTS radical(IC_(50)=89.60±8.73μg/mL)as well as significant inhibition of xanthine oxidase(IC_(50)=47.9±2.04μg/mL)and lipid peroxidation(IC_(50)=231.63±5.21μg/mL).ADEO also showed significant anti-inflammatory activity by inhibiting the enzymesCOX-1(IC_(50)=59.2±2.43μg/mL),Cyclo-oxygenase-2(COX-2)(IC_(50)=74.68±1.34μg/mL)and 5-lipooxygense(5-LOX)(IC_(50)=93.18±1.87μg/mL),which are involved in the inflammatory pathway.These findings suggest that ADEO,with its high estragole content,holds promising potential as a natural antibacterial,antioxidant,and anti-inflammatory agent for preventive and therapeutic applications.Further research is needed to explore its safety and efficacy in clinical settings.
基金supported by National Key R&D Program of China(2023YFD2100205)Fuzhou Science&Technology Project,China(2022-Y-0022022-P-023).
文摘Identification of natural substances with antioxidant properties is ongoing research for addressing issues related to oxidative stress especially attributed to environmental effects.Our previous study demonstrated that Lateolabrax japonicus peptides(LPH),rich in Glu,Gly,and hydrophobic amino acids,exhibited remarkable antioxidant activity in vitro,with though its action mechanism yet to be revealed.Therefore,to assess the in vivo antioxidative properties of LPH,we employed H_(2)O_(2) to generate oxidative stress in Drosophila melanogaster model.Results indicated that LPH significantly prolonged the lifespan of Drosophila subjected to oxidative stress mostly mediated via LPH’s enhancement of the antioxidant defense system and intestinal functions.Antioxidant effects were manifested by a decrease in malondialdehyde(MDA)levels,elevated superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities,decreased levels of reactive oxygen species(ROS)in intestinal epithelial cells,and the preservation of intestinal length.LPH effectively controlled the excessive proliferation and differentiation of oxidative stress-induced Drosophila intestinal stem cells.At the gene level,LPH upregulated the expression of antioxidant-related Nrf2 genes while concurrently downregulated mTOR expression level.Furthermore,high-throughput 16S rDNA sequencing revealed that the addition of LPH significantly influenced the diversity and abundance of the intestinal microbiota in H_(2)O_(2)-induced Drosophila.These findings provide a deeper understanding of the antioxidative mechanism of LPH,suggesting its potential applications in food industry and to be assessed using other in vivo oxidative stress models.
基金financially supported by the National Key R&D Program of China(No.2022YFB3808000)the National Natural Science Foundation of China(No.U21A2099)+2 种基金the Science and Technology Development Program of Jilin Province(No.20240101002JJ)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.Y2023066)the Plan for Enhancing Health Science and Technology Capacity in Jilin Province(No.2020J041).
文摘Oxidative stress,characterized by the excessive accumulation of reactive oxygen species(ROS),is linked to various pathological conditions,including myocardial infarction,cancer,and neurodegenerative diseases.Addressing ROS-induced cell damage has become a critical focus of biomedical research.In this study,a thermo-sensitive poly(amino acid)hydrogel,composed of poly(ethylene glycol)-block-poly(l-methionine),was prepared for cytoprotection through ROS scavenging.The sol-to-gel transition mechanism of the hydrogel was elucidated,and its potent antioxidant properties and cell protective effects were validated using hydrogen peroxide(H_(2)O_(2))-induced oxidative stress and oxygen-glucose deprivation(OGD)models.The hydrogel significantly mitigated H_(2)O_(2)-induced damage in L929 cells,doubling their survival rate.Additionally,it scavenged approximately 35.8%of the ROS during OGD,reducing mitochondrial oxidative damage and resulting in a 29.4%decrease in apoptotic cell number.These findings underscore the potential biomedical applications of thermo-sensitive poly(amino acid)hydrogels,particularly in treating oxidative stress-related cell damage.
基金support from the Beijing Natural Science Foundation(No.JQ23035)。
文摘High-efficient rubber antioxidants for enhanced heat resistance without compromising mechanical properties remain an enormous and long-term challenge for the rubber industry.Herein,we employed the in-situ growth of Ce-doped Co-metal-organic framework(Ce Co-MOF)in dendritic mesoporous organosilica nanoparticles(DMONs@Ce Co-MOF,denoted as DCCM)to prepare a novel antioxidant that exhibit outstanding thermal stability.Dendritic mesoporous organosilica nanoparticles(DMONs)effectively alleviated the incompatibility of Ce Co-MOF in the polymer matrix,and the effective scavenging of free radicals was attributed to the various oxidation states of metal ions in Ce Co-MOF.Surprising,by adding only0.5 phr(parts per hundred of rubber)of DMONs@Ce Co-MOF to silicone rubber,(SR),the retention rate of tensile strength increased from 37.3%to 61.6%after aging 72 h at 250℃,and the retention rate of elongation at break of DCCM/SR1 composites reached 68%,which was 5.43 times of SR.The strategy of anchoring MOFs on the surface of silica also provides a viable method for preparing effective compound functionalized rubber antioxidant.
基金Supported by Scientific and Technological Project of Heilongjiang Province(GC05B205)Scientific and Technological Project of Heilongjiang Land Reclamation Bureau(HNKXV-03-04-06A)~~
文摘[Objective] The aim of the study was to determine whether phytotoxicity of TeA against Echinochloa crus-galli leaves was correlated with oxidative stress caused by generation of reactive oxygen and the changes of antioxidant enzymes activity.[Method] The changes of malondialdehyde（MDA）content,hydrogen peroxide（H2O2）,and activities of superoxide dismutase（SOD）,glutathione reductase（GR）and catalase（CAT）were studied by leaf segment method in vitro.[Result] After the treatment of 500 μmol/L TeA,the content of MDA and H2O2 increased by 247.86% and 67.00%,respectively,indicating that the accumulation of MDA and H2O2 in E.crus-galli leaves was due to the reactive oxygen burst induced by TeA.TeA induced a significant increase in activities of SOD,GR and CAT.At 500 μmol/L TeA,activities of SOD,GR and CAT increased more than one fold compared with the control.[Conclusion] TeA could not only cause oxidative stress in leaves of E.crus-galli through the induction of reactive oxygen,but also induce the increasing of antioxidant enzyme activity.
基金financially supported by the National Natural Science Foundation of China(No.52336009)Guangdong Basic and Applied Basic Research Foundation(No.2023A1515010429)+4 种基金the Natural Science Basic Research Plan in Shaanxi Province of China(No.2024JC-YBQN-0475)Xidian University Specially Funded Project for Interdisciplinary Exploration(No.TZJH2024063)the Fundamental Research Funds for the Central Universities(No.QTZX23061)Qinchuangyuan Project(No.QCYRCXM-2022-236)the Innovation Center of Nuclear Power Technology(No.HDLCXZX-2022-ZH-013)
文摘Solid oxide fuel cell(SOFC)is a promising energy conversion device because of its high efficiency and fuel/oxidant flexibility,enabling the direct conversion of chemical energy to electrical power.By using N_(2)O as an oxidant instead of air,we develop a dual-phase catalyst La_(0.6)Sr_(0.4)Co_(0.2)Fe_(0.8)O_(3-δ)-Gd_(0.1)Ce_(0.9)O_(2-δ)(LSCF-GDC),which exhibits efficient bifunctionality as a cathode for SOFC,synergistic ally promoting simultaneous N_(2)O decomposition and oxygen reduction kinetics.
文摘The article by Mansouri et al provides a comprehensive investigation into the effects of L-arginine(L-Arg)on diabetic cardiomyopathy.The authors conclude that while a low dose(0.5 g/kg)of L-Arg improves lipid profiles and reduces body weight,higher doses(≥1 g/kg)exacerbate oxidative stress,inflammation,and myocardial damage.In this letter,we aim to expand on the potential role of anti-inflammatory and antioxidant strategies in mitigating these adverse effects.Specifically,we focus on nuclear factor erythroid 2-related factor 2 activation and nitric oxide synthase modulation.These strategies could enhance the clinical utility of L-Arg by preserving its metabolic benefits while reducing its cardiotoxic risks.We believe this perspective will stimulate future research on L-Arg-based therapies in patients with diabetes,with an emphasis on optimizing dosage and exploring synergistic co-therapies.
基金funded by the Ghent University Special Research Fund(BOF.PDO.2022.0002.01)Projects of International Cooperation of Henan Province(232102520016)National Natural Science Foundation of Henan Province(242300420159).
文摘Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS.Results Implementing cyclic chronic HS(34 ℃ for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the m RNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis.Conclusions These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers.
文摘Background:Ischemic stroke is a disease characterized by the damage of brain tissue due to insufficient blood supply.The neuronal necrosis caused by oxidative stress during the acute phase of ischemic stroke leads to serious consequences,including blood-brain barrier disruption and vascular aging.The Kelch-like ECH-associated protein 1(KEAP1),is a key switch of antioxidative system in human body.Until now,there is still a lack of effective treatment to ischemic stroke.Methods:We developed scutellarin-based liposomes for treating ischemic stroke injury caused neuronal damage.Results:The results showed that scutellarin could directly bind to KEAP1 protein,and the Kd was 26.1μM.The scutellarin-based liposomes significantly reduced cellular reactive oxygen species(ROS)levels.It could also upregulate the protein expression level of nuclear factor E2-related factor 2(NRF2),which is the substrate protein of KEAP1.Next,both the mRNA and protein expression level of the NRF2 downstream anti-oxidative element,heme oxygenase 1(HO-1)and NAD(P)H quinone dehydrogenase 1(NQO1)were promoted.Furthermore,the coimmunoprecipitation(Co-IP)and hydrogen-deuterium exchange mass spectrometry(HDX-MS)revealed that scutellarin directly bound to KEAP1’s Kelch domain,interrupting the interaction between KEAP1 and NRF2.Conclusion:Our work indicates that the scutellarin-based liposomes might be a promising therapeutic approach for ischemic stroke induced neuronal necrosis.
文摘Thymus serpyllum L.,often known as wild thyme,has been used since ancient times due to its multifaceted culinary and medicinal attributes.It is usually utilized in folk medicine to manage different health issues.This work aimed to investigate the chemical composition and biological characteristics of T.serpyllum essential oil(EO),including its antimicrobial,antioxidant,and anti-inflammatory capabilities.Moreover,we have prompted an in-silico simulation to reveal the underlying mode of action of these properties.The chemical characterization of T.serpyllum(EO)by Gas Chromatography-Mass Spectrometry(GC-MS)indicated sabinene(17.33%),terpinen-4-ol(11.73%),phellandral(13.18%),and thymol(10.54%)as main components.The antimicrobial screening utilized the disc-diffusion technique,MIC,and MBC assays.The disc-diffusion test’s results revealed significant anti-Candida activity and notable antibacterial efficacy.The MIC and MBC tests showed that T.serpyllum EO effectively stops bacterial growth,including Gram-positive and Gram-negative strains and Candida strains.The tolerance level ratio demonstrated that this EO exhibits bactericidal and fungicidal effects on all tested bacteria and Candida strains.Also,T.serpyllum EO presented effective inhibitory activity against the 5-lipoxygenase(5-LOX)enzyme(IC50=744.19±0.1µg/mL)(p<0.05).It also effectively affected FRAP,β-carotene,DPPH,and ABTS radicals.In light of these findings,T.serpyllum holds promise for diverse applications across pharmaceuticals,nutraceuticals,and the food industry.However,further research and collaboration between traditional knowledge and modern medicine are crucial to fully realizing its potential benefits in these fields.
基金Researchers Supporting Project No.(RSP2025R390),King Saud University,Riyadh,Saudi Arabia.
文摘Nickel(Ni)toxicity significantly impairs plant growth,photosynthesis,and metabolism by inducing oxidative stress.This study evaluates the potential of exogenous Alpha-Ketoglutarate(AKG)in mitigating Ni-induced stress in Peganum harmala L.Seedlings were exposed to 0,200,500,and 750μM NiCl2,with or without AKG supplementation.Under 750μM Ni stress,dry weight(DW)decreased by 33.7%,tissue water content(TWC)by 39.9%,and chlorophyll a and total chlorophyll levels were reduced by 17%and 15%,respectively.Ni exposure also significantly increased secondary metabolite production,with leaf anthocyanin content rising by 131%,and superoxide dismutase(SOD)and catalase(CAT)activities increasing by 228%and 53%,respectively,in roots at 500μM Ni.AKG treatment alleviated Ni toxicity by enhancing TWC by 39%and promoting root and shoot growth.Additionally,AKG treatment boosted the synthesis of phenolic compounds and flavonoids,contributing to improved tolerance against Ni stress.These findings demonstrate the potential of AKG in enhancing Ni tolerance in P.harmala,suggesting its promising role in bioremediation of metal-contaminated soils.This is the first study to report the beneficial effects of exogenous AKG in alleviating nickel toxicity in P.harmala L.,offering a new approach for improving plant resilience to heavy metal stress.
基金the Department of Zoology,Visva-Bharati for providing infrastructural supportSreejata Kamila is grateful to the Council of Scientific and Industrial Research(CSIR),India for Senior Research Fellowship(CSIR File No.09/202(0102)/2019-EMR-I)+2 种基金Koushik Kumar Dey acknowledges the Department of Biotechnology,New Delhi for research fellowship(No.BT/PR28560/AAQ/3/919/2018)Shehnaz Islam is thankful to Maulana Azad National Fellowship(UGC ref ID-201920–345938)India for her financial support.
文摘Arsenic(As)and chromium(Cr)are two harmful toxicants as well as carcinogens which can coexist in polluted surface water and groundwater.This coexistence leads to mixture effects in animals including fish.Both of these heavy metals are reported to manifest reactive oxygen species(ROS)mediated toxicity.Though individual neurotoxic effects have been reported,their mixture effects,its mechanism and cellular responses against oxidative stress and DNA damages remain unknown.The present study evaluated the individual and mixture effects of As and Cr at their environmentally relevant concentrations in zebrafish(Danio rerio)brain after 15,30 and 60 days of exposure.Nrf2,a transcription factor is involved in the expressional regulation of enzymes needed to maintain cellular redox homeostasis.This study reported the expressional pattern of Nrf2 and its associated xenobiotic metabolizing enzyme Nqo1 and other markers of oxidative stress such as ROS generation,reduced glutathione level,lipid peroxidation and catalase activity.Increased malondialdehyde(MDA)content,glutathione level,and catalase activity indicated oxidative stress in exposed groups.In addition,this study revealed expressional alterations of neurotoxicity marker(ache),DNA repair(ogg1,apex1,creb1,polb,mlh1,msh2 and msh6)and tumor suppressor(p53,brca2)genes.Results of ROS generation,MDA level,histopathological analysis,gene expression and immunofluorescence study confirmed that As and Cr did not show antagonistic effects in combination rather indicated additive effects which was dose-dependent but not always linear.
基金financially sponsored by the State Key Research Development Program of China(Nos.2021YFB3800700 and 2021YFB3800703)the National Natural Science Foundation of China(Nos.52122306 and U21A2098)the Sichuan Province Science and Technology Program(No.2022NSFSC0382).
文摘Oxidative stress is a critical risk factor for various disease complications in patients with end-stage renal disease,which may be further aggravated during hemodialysis.Herein,we prepared a hemodialysis membrane by introducing poly(2-Acrylamide-2-methylpropanesulfonic acid-N-vinylpyrrolidone)(P(AMPS-VP))into the polyethersulfone(PES)membrane by simple in-situ polymerization and non-solvent phase sepa-ration(NIPS)method,followed by tannic acid(TA)coating construction through hydrogen bonding interaction,termed PES/P(AMPS-VP)-TA.The membrane can efficiently remove reactive nitrogen radicals(RNS)(DPPH•,89.96%;ABTS•+,90.49%)and reactive oxygen species(ROS)(O_(2)•^(−),90.45%),and has a very sta-ble antioxidant property.Meanwhile,the membrane does not cause hemolysis and coagulation,and has superior blood compatibility required by hemodialysis membrane.In addition,the dialysis performance of the membrane is improved compared with unmodified PES,which is beneficial for practical hemodialysis applications.This work provides a reference for industrial preparation of hemodialysis membranes,which is expected to be applied to reduce oxidative stress in hemodialysis patients.
基金supported by the National Natural Science Foundation of China(82160803)High level Innovative Talents Project of Guizhou Province(Guizhou Science and Technology Cooperation Platform Talents-GCC[2023]077).
文摘Background:To explore the antioxidant capacity ofα-asarone(ARE)in mice models of sodium valproate(SV)-induced liver injury,and to elucidate the underlying mechanism of ARE in liver injury treatment.Methods:Network pharmacology and molecular docking were used to predict ARE’s potential pharmacological mechanisms in treating drug-induced liver injury.AML12 cell model was used to evaluate the antioxidant stress activation of ARE.In the evaluation of antioxidant activity,ARE was tested for its ability to scavenge hydroxyl radical(OH)radicals,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+),and 1,1-diphenyl-2-picrylhydrazyl(DPPH),with vitamin C serving as the positive control.And thirty-six male mice were divided into six groups.Group I,the control,received saline.The other five groups received oral doses of 500 mg/kg/b.w.of SV daily for 14 days.Group II received only SV.Group III received the hepatic protectant bifendate at 100 mg/kg/b.w.,while groups IV,V,and VI were treated with 20,40,and 80 mg/kg/b.w.of ARE,respectively,also for 14 days.After sacrifice,liver and blood samples were collected for biochemical or metabolomic analysis.Results:ARE significantly reduced the levels of reactive oxygen species and activated the Keap1/Nrf2 signaling pathway in liver injury AML12 cells.ARE demonstrated dose-dependent scavenging activity in DPPH,ABTS+,and OH assays.It lowered serum alanine aminotransferase and aspartate aminotransferase levels,increased glutathione peroxidase and superoxide dismutase activity in liver tissue,and reduced malondialdehyde content.Metabolomic analysis showed that ARE restored twenty liver metabolites to control levels and enriched three pathways(arginine biosynthesis,sucrose and starch metabolism,and biotin metabolism)with shared differential metabolites.Conclusion:These results shed light on ARE’s mechanism in reducing oxidative stress,suggesting a potential strategy for preventing SV-induced liver disease by modulating metabolic products and enhancing antioxidant capacity.ARE could be a promising drug candidate for its antioxidant and liver-protective effects.
基金supported by the Postdoctoral Fellowship Program of CPSF(GZC20230017)the Natural Science Research Project of Anhui Educational Committee(2024AH050153)+1 种基金the National Natural Science Foundation of China(52322407,52274313)the China Postdoctoral Science Foundation(2024M750013).
文摘This study explores the unique role of CO_(2)as an oxidant in stainless steel smelting,focusing on its effectiveness in decarbonization and chromium retention.The research begins by theoretically demonstrating that although the introduction of CO_(2)increases the CO partial pressure in the reaction system,the decarburization and chromium(Cr)retention capabilities of CO_(2)can still be stably maintained through the rational adjustment of the molten steel composition,temperature,and inert gas proportions.Further experimental findings indicate that chromium does not exhibit significant oxidation losses when the carbon(C)content exceeds 1.0%(mass).Finally,a novel CO_(2)recovery and utilization approach is proposed,integrating CO_(2)capture from smelting flue gas and recycling it for smelting,reducing O_(2)consumption and energy costs.This innovative process,compatible with existing smelting plants,presents a promising pathway towards carbon neutrality in the iron and steel industry,bridging theoretical insights with practical applications.
