The optical manipulation of nanoparticles on superlubricity surfaces was investigated.The research revealed that,due to the near-zero static friction and extremely low dynamic friction at superlubricity interfaces,the...The optical manipulation of nanoparticles on superlubricity surfaces was investigated.The research revealed that,due to the near-zero static friction and extremely low dynamic friction at superlubricity interfaces,the maximum intensity for controlling the optical field can be less than 100 W/cm^(2).The controlled nanoparticle radius can be as small as 5 nm,which is more than one order of magnitude smaller than that of nanoparticles controlled through traditional optical manipulation.Manipulation can be achieved on sub-microsecond to microsecond timescales.Furthermore,the manipulation takes place on solid surfaces and in nonliquid environments,with minimal impact from Brownian motion.By appropriately increasing the dynamic friction,controlling the light intensity,or reducing the pressure,the effects of Brownian motion can be eliminated,allowing for the construction of microstructures with a size as small as 1/75 of the wavelength of light while controlling the light intensity,which is seven orders of magnitude smaller compared to manipulating nanoparticles on traditional surfaces.This enables the control of super-resolution optical microstructures.The optical super-resolution manipulation of nanoparticles on superlubricity surfaces has important applications in fields such as nanofabrication,photolithography,optical metasurfaces,and biochemical analysis.展开更多
The photo-kinetics of fluorescent molecules have enabled the circumvention of the far-field optical diffraction limit.Despite its enormous potential,the necessity to label the sample may adversely influence the delica...The photo-kinetics of fluorescent molecules have enabled the circumvention of the far-field optical diffraction limit.Despite its enormous potential,the necessity to label the sample may adversely influence the delicate biology under investigation.Thus,continued development efforts are needed to surpass the far-field label-free diffraction barrier.The statistical similarity or finite coherence of the scattered light off the sample in label-free mode hinders the application of existing super-resolution methods based on incoherent fluorescence imaging.In this article,we present physics and propose a methodology to circumvent this challenge by exploiting the photoluminescence(PL)of silicon nitride waveguides for near-field illumination of unlabeled samples.The technique is abbreviated EPSLON,Evanescently decaying Photoluminescence Scattering enables Label-free Optical Nanoscopy.We demonstrate that such an illumination has properties that mimic the photo-kinetics of nano-sized fluorescent molecules,i.e.,such an illumination permits incoherence between the scattered fields from various locations on the sample plane.Thus,the illumination scheme enables the development of a far-field label-free incoherent imaging system that is linear in intensity and stable over time,thereby permitting the application of techniques like structured illumination microscopy(SIM)and intensity-fluctuation-based optical nanoscopy(IFON)in label-free mode to circumvent the diffraction limit.In this proof-of-concept work,we observed a two-point resolution of~180 nm on super-resolved nanobeads and resolution improvements between 1.9×to 2.8×over the diffraction limit,as quantified using Fourier Ring Correlation(FRC),on various biological samples.We believe EPSLON is a step forward within the field of incoherent far-field label-free super-resolution microscopy that holds a key to investigating biological systems in their natural state without the need for exogenous labels.展开更多
Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the...Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes： （1） patterned illumination- based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; （2） single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluo- rophore at subdiffraction precision; （3） bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)(Nos.62174040 and 12174423)the 13th Batch of Outstanding Young Scientific and Technological Talents Project in Guizhou Province(No.[2021]5618)+1 种基金the Science and Technology Projects of Guizhou Provincial(No.ZK[2024]501)the Scientific Research Fund of Guizhou Minzu University(No.GZMUZK[2023]CXTD07).
文摘The optical manipulation of nanoparticles on superlubricity surfaces was investigated.The research revealed that,due to the near-zero static friction and extremely low dynamic friction at superlubricity interfaces,the maximum intensity for controlling the optical field can be less than 100 W/cm^(2).The controlled nanoparticle radius can be as small as 5 nm,which is more than one order of magnitude smaller than that of nanoparticles controlled through traditional optical manipulation.Manipulation can be achieved on sub-microsecond to microsecond timescales.Furthermore,the manipulation takes place on solid surfaces and in nonliquid environments,with minimal impact from Brownian motion.By appropriately increasing the dynamic friction,controlling the light intensity,or reducing the pressure,the effects of Brownian motion can be eliminated,allowing for the construction of microstructures with a size as small as 1/75 of the wavelength of light while controlling the light intensity,which is seven orders of magnitude smaller compared to manipulating nanoparticles on traditional surfaces.This enables the control of super-resolution optical microstructures.The optical super-resolution manipulation of nanoparticles on superlubricity surfaces has important applications in fields such as nanofabrication,photolithography,optical metasurfaces,and biochemical analysis.
文摘The photo-kinetics of fluorescent molecules have enabled the circumvention of the far-field optical diffraction limit.Despite its enormous potential,the necessity to label the sample may adversely influence the delicate biology under investigation.Thus,continued development efforts are needed to surpass the far-field label-free diffraction barrier.The statistical similarity or finite coherence of the scattered light off the sample in label-free mode hinders the application of existing super-resolution methods based on incoherent fluorescence imaging.In this article,we present physics and propose a methodology to circumvent this challenge by exploiting the photoluminescence(PL)of silicon nitride waveguides for near-field illumination of unlabeled samples.The technique is abbreviated EPSLON,Evanescently decaying Photoluminescence Scattering enables Label-free Optical Nanoscopy.We demonstrate that such an illumination has properties that mimic the photo-kinetics of nano-sized fluorescent molecules,i.e.,such an illumination permits incoherence between the scattered fields from various locations on the sample plane.Thus,the illumination scheme enables the development of a far-field label-free incoherent imaging system that is linear in intensity and stable over time,thereby permitting the application of techniques like structured illumination microscopy(SIM)and intensity-fluctuation-based optical nanoscopy(IFON)in label-free mode to circumvent the diffraction limit.In this proof-of-concept work,we observed a two-point resolution of~180 nm on super-resolved nanobeads and resolution improvements between 1.9×to 2.8×over the diffraction limit,as quantified using Fourier Ring Correlation(FRC),on various biological samples.We believe EPSLON is a step forward within the field of incoherent far-field label-free super-resolution microscopy that holds a key to investigating biological systems in their natural state without the need for exogenous labels.
基金supported by the grants from the National Natural Science Foundation of China(Nos.11174089 and 61138003)the Instrument Developing Project of the Chinese Academy of Sciences(No.YZ201263)+2 种基金the Instrument Function Developing Project of the Chinese Academy of Sciences(No.yg2012032)the Key Project of Department of Education of Guangdong Province(No.cxzd1112)Guangzhou Municipal Science and Technology Program Project(No.2012J5100004)
文摘Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes： （1） patterned illumination- based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; （2） single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluo- rophore at subdiffraction precision; （3） bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.
