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基于CSDBO-BP的TC4钛合金铣削预测模型及多目标优化
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作者 张春 蒋政泉 +3 位作者 郗琳 郎广辉 赵俊花 李丽 《西南大学学报(自然科学版)》 北大核心 2026年第1期250-265,共16页
为降低钛合金铣削加工过程中的加工能耗和铣削负载,以加工能耗和铣削合力最小为目标构建预测模型并开展多目标优化研究。首先,设计单因素实验分析了钛合金铣削加工过程中切削参数的影响规律;其次,将纵横交叉策略改进的蜣螂算法(Dung Bee... 为降低钛合金铣削加工过程中的加工能耗和铣削负载,以加工能耗和铣削合力最小为目标构建预测模型并开展多目标优化研究。首先,设计单因素实验分析了钛合金铣削加工过程中切削参数的影响规律;其次,将纵横交叉策略改进的蜣螂算法(Dung Beetle Optimization Algorithm Incorporating Criss-cross Strategies)与BP(Back Propagation)神经网络相结合,建立CSDBO-BP神经网络预测模型;最后,将预测模型与遗传算法相结合寻找切削参数的最优组合。实验结果表明:CSDBO-BP神经网络预测模型的预测精度达97%以上;多目标优化可使钛合金铣削过程中的加工能耗减少18.31%,铣削合力减少34.16%。 展开更多
关键词 钛合金 预测模型 多目标优化 混合算法
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FMCSNet: Mobile Devices-Oriented Lightweight Multi-Scale Object Detection via Fast Multi-Scale Channel Shuffling Network Model
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作者 Lijuan Huang Xianyi Liu +1 位作者 Jinping Liu Pengfei Xu 《Computers, Materials & Continua》 2026年第1期1292-1311,共20页
The ubiquity of mobile devices has driven advancements in mobile object detection.However,challenges in multi-scale object detection in open,complex environments persist due to limited computational resources.Traditio... The ubiquity of mobile devices has driven advancements in mobile object detection.However,challenges in multi-scale object detection in open,complex environments persist due to limited computational resources.Traditional approaches like network compression,quantization,and lightweight design often sacrifice accuracy or feature representation robustness.This article introduces the Fast Multi-scale Channel Shuffling Network(FMCSNet),a novel lightweight detection model optimized for mobile devices.FMCSNet integrates a fully convolutional Multilayer Perceptron(MLP)module,offering global perception without significantly increasing parameters,effectively bridging the gap between CNNs and Vision Transformers.FMCSNet achieves a delicate balance between computation and accuracy mainly by two key modules:the ShiftMLP module,including a shift operation and an MLP module,and a Partial group Convolutional(PGConv)module,reducing computation while enhancing information exchange between channels.With a computational complexity of 1.4G FLOPs and 1.3M parameters,FMCSNet outperforms CNN-based and DWConv-based ShuffleNetv2 by 1%and 4.5%mAP on the Pascal VOC 2007 dataset,respectively.Additionally,FMCSNet achieves a mAP of 30.0(0.5:0.95 IoU threshold)with only 2.5G FLOPs and 2.0M parameters.It achieves 32 FPS on low-performance i5-series CPUs,meeting real-time detection requirements.The versatility of the PGConv module’s adaptability across scenarios further highlights FMCSNet as a promising solution for real-time mobile object detection. 展开更多
关键词 object detection lightweight network partial group convolution multilayer perceptron
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Face-Pedestrian Joint Feature Modeling with Cross-Category Dynamic Matching for Occlusion-Robust Multi-Object Tracking
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作者 Qin Hu Hongshan Kong 《Computers, Materials & Continua》 2026年第1期870-900,共31页
To address the issues of frequent identity switches(IDs)and degraded identification accuracy in multi object tracking(MOT)under complex occlusion scenarios,this study proposes an occlusion-robust tracking framework ba... To address the issues of frequent identity switches(IDs)and degraded identification accuracy in multi object tracking(MOT)under complex occlusion scenarios,this study proposes an occlusion-robust tracking framework based on face-pedestrian joint feature modeling.By constructing a joint tracking model centered on“intra-class independent tracking+cross-category dynamic binding”,designing a multi-modal matching metric with spatio-temporal and appearance constraints,and innovatively introducing a cross-category feature mutual verification mechanism and a dual matching strategy,this work effectively resolves performance degradation in traditional single-category tracking methods caused by short-term occlusion,cross-camera tracking,and crowded environments.Experiments on the Chokepoint_Face_Pedestrian_Track test set demonstrate that in complex scenes,the proposed method improves Face-Pedestrian Matching F1 area under the curve(F1 AUC)by approximately 4 to 43 percentage points compared to several traditional methods.The joint tracking model achieves overall performance metrics of IDF1:85.1825%and MOTA:86.5956%,representing improvements of 0.91 and 0.06 percentage points,respectively,over the baseline model.Ablation studies confirm the effectiveness of key modules such as the Intersection over Area(IoA)/Intersection over Union(IoU)joint metric and dynamic threshold adjustment,validating the significant role of the cross-category identity matching mechanism in enhancing tracking stability.Our_model shows a 16.7%frame per second(FPS)drop vs.fairness of detection and re-identification in multiple object tracking(FairMOT),with its cross-category binding module adding aboute 10%overhead,yet maintains near-real-time performance for essential face-pedestrian tracking at small resolutions. 展开更多
关键词 Cross-category dynamic binding joint feature modeling face-pedestrian association multi object tracking occlusion robustness
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EHDC-YOLO: Enhancing Object Detection for UAV Imagery via Multi-Scale Edge and Detail Capture
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作者 Zhiyong Deng Yanchen Ye Jiangling Guo 《Computers, Materials & Continua》 2026年第1期1665-1682,共18页
With the rapid expansion of drone applications,accurate detection of objects in aerial imagery has become crucial for intelligent transportation,urban management,and emergency rescue missions.However,existing methods ... With the rapid expansion of drone applications,accurate detection of objects in aerial imagery has become crucial for intelligent transportation,urban management,and emergency rescue missions.However,existing methods face numerous challenges in practical deployment,including scale variation handling,feature degradation,and complex backgrounds.To address these issues,we propose Edge-enhanced and Detail-Capturing You Only Look Once(EHDC-YOLO),a novel framework for object detection in Unmanned Aerial Vehicle(UAV)imagery.Based on the You Only Look Once version 11 nano(YOLOv11n)baseline,EHDC-YOLO systematically introduces several architectural enhancements:(1)a Multi-Scale Edge Enhancement(MSEE)module that leverages multi-scale pooling and edge information to enhance boundary feature extraction;(2)an Enhanced Feature Pyramid Network(EFPN)that integrates P2-level features with Cross Stage Partial(CSP)structures and OmniKernel convolutions for better fine-grained representation;and(3)Dynamic Head(DyHead)with multi-dimensional attention mechanisms for enhanced cross-scale modeling and perspective adaptability.Comprehensive experiments on the Vision meets Drones for Detection(VisDrone-DET)2019 dataset demonstrate that EHDC-YOLO achieves significant improvements,increasing mean Average Precision(mAP)@0.5 from 33.2%to 46.1%(an absolute improvement of 12.9 percentage points)and mAP@0.5:0.95 from 19.5%to 28.0%(an absolute improvement of 8.5 percentage points)compared with the YOLOv11n baseline,while maintaining a reasonable parameter count(2.81 M vs the baseline’s 2.58 M).Further ablation studies confirm the effectiveness of each proposed component,while visualization results highlight EHDC-YOLO’s superior performance in detecting objects and handling occlusions in complex drone scenarios. 展开更多
关键词 UAV imagery object detection multi-scale feature fusion edge enhancement detail preservation YOLO feature pyramid network attention mechanism
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Deep Learning-Based Toolkit Inspection: Object Detection and Segmentation in Assembly Lines
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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db/db小鼠2型糖尿病骨质疏松模型的研究
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作者 赵奕 冯秀芝 +5 位作者 王智民 陈怡然 杨潇 任艳玲 柳京池 曹文琪 《中国骨质疏松杂志》 北大核心 2025年第1期137-141,共5页
db/db小鼠是广泛应用于2型糖尿病及其并发症实验研究的动物模型,对评价新药药效及阐明病理生理学机制的研究具有重要的意义。该文通过检索中英文数据库梳理db/db小鼠的遗传背景和病理特征,并收集整理了以db/db小鼠为2型糖尿病性骨质疏... db/db小鼠是广泛应用于2型糖尿病及其并发症实验研究的动物模型,对评价新药药效及阐明病理生理学机制的研究具有重要的意义。该文通过检索中英文数据库梳理db/db小鼠的遗传背景和病理特征,并收集整理了以db/db小鼠为2型糖尿病性骨质疏松症模型开展的现代药物、中药及相关临床应用的基础研究,以期为今后本病的实验研究提供可靠的证据。 展开更多
关键词 db/db小鼠 2型糖尿病性骨质疏松症 动物模型 模型应用
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益肾降糖饮调控内质网应激介导NLRP3炎症小体改善糖尿病肾病db/db小鼠肾损伤的作用机制研究 被引量:3
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作者 杨运劼 叶彬华 +7 位作者 邱晨 吴涵卿 黄博威 王桐 阮诗玮 郭芳 王建挺 江铭倩 《中国中药杂志》 北大核心 2025年第10期2740-2749,共10页
旨在探讨益肾降糖饮调控内质网应激(ERS)介导NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎症小体改善糖尿病肾病db/db小鼠肾损伤的作用机制。将30只db/db小鼠随机分为模型组、益肾降糖饮组、ERS抑制剂4-苯基丁酸(4-PBA)组,每组10只,另选10... 旨在探讨益肾降糖饮调控内质网应激(ERS)介导NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎症小体改善糖尿病肾病db/db小鼠肾损伤的作用机制。将30只db/db小鼠随机分为模型组、益肾降糖饮组、ERS抑制剂4-苯基丁酸(4-PBA)组,每组10只,另选10只db/m小鼠作为对照组。益肾降糖饮组予益肾降糖饮0.01 mL·g^(-1)灌胃,4-PBA组予4-PBA 0.5 mg·g^(-1)灌胃,对照组和模型组予等体积羧甲基纤维素钠灌胃,每日1次,连续8周,每2周记录各组小鼠摄食量、饮水量及体质量。干预结束后检测各组小鼠空腹血糖(FBG)、糖化血红蛋白(HbA1c)、尿微量白蛋白(U-mALB)、24 h尿量、血肌酐(Scr)、尿素氮(BUN),酶联免疫吸附测定(ELISA)法检测炎症指标白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平,苏木精-伊红(HE)、过碘酸-雪夫(PAS)、马松(Masson)染色及透射电镜(TEM)观察肾组织病理,蛋白免疫印迹法检测各组肾组织中葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)、NLRP3、含有CARD结构域的凋亡相关斑点样蛋白(ASC)、含半胱氨酸的天冬氨酸蛋白水解酶1(caspase-1)和消皮素D(GSDMD)的表达。结果显示,与对照组相比,模型组小鼠一般状态不佳,体质量、摄食量、饮水量、FBG、HbA1c、U-mALB、肾脏指数、24 h尿量、IL-1β、IL-18水平明显升高。与模型组相比,益肾降糖饮组和4-PBA组小鼠一般状态改善,体质量升高,摄食量、FBG、U-mALB、肾脏指数、24 h尿量、IL-1β水平显著下降;益肾降糖饮组IL-18水平显著下降;4-PBA组饮水量、HbA1c显著下降;益肾降糖饮组饮水量、HbA1c虽有下降趋势,但差异无统计学意义。各组BUN、Scr、肾脏质量无显著差异。肾脏病理染色显示,模型组较对照组肾脏病理损伤严重,可见肾小球系膜弥漫性增生,肾小管水肿,管腔扩张,肾间质见大量炎性细胞浸润,基底膜见糖原染色及蓝色胶原沉积明显增多;益肾降糖饮组及4-PBA组可见肾脏损伤、糖原染色及胶原沉积不同程度改善,其中益肾降糖饮组改善更明显。TEM提示模型组肾组织胞质大面积水肿,基底膜均质性增厚,足突数量较少,多数融合变宽;益肾降糖饮组及4-PBA组肾组织胞质水肿减轻、基底膜完整均匀,足突融合改善。蛋白免疫印迹检测结果表明,与对照组相比,模型组GRP78、CHOP、GSDMD、NLRP3、ASC、caspase-1表达上调;与模型组相比,益肾降糖饮组、4-PBA组GRP78、CHOP、GSDMD、NLRP3、ASC、caspase-1表达下调。上述研究表明,益肾降糖饮通过抑制ERS减轻NLRP3炎症小体活化,改善db/db小鼠炎症反应,从而发挥其保护糖尿病肾病的作用。 展开更多
关键词 益肾降糖饮 糖尿病肾病 内质网应激 NLRP3炎症小体 db/db小鼠 炎症反应
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基于NLRP3炎症小体介导的细胞焦亡探讨丹蛭降糖胶囊对db/db小鼠心肌损伤的影响 被引量:4
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作者 阮诺冰 李金菊 +3 位作者 许奇 经加吻 高家荣 方朝晖 《中国药理学通报》 北大核心 2025年第4期786-792,共7页
目的基于NLRP3炎症小体介导的细胞焦亡,探讨丹蛭降糖胶囊(Danzhi Jiangtang capsules,DJC)对db/db小鼠心肌保护作用的可能机制。方法将db/db小鼠随机分为模型组、DJC低、中、高剂量组及二甲双胍组,另取db/m小鼠为对照组,连续给药8周。... 目的基于NLRP3炎症小体介导的细胞焦亡,探讨丹蛭降糖胶囊(Danzhi Jiangtang capsules,DJC)对db/db小鼠心肌保护作用的可能机制。方法将db/db小鼠随机分为模型组、DJC低、中、高剂量组及二甲双胍组,另取db/m小鼠为对照组,连续给药8周。给药结束后,检测各组小鼠血糖、血脂、心肌酶指标及炎症因子水平;HE及Masson染色观察心肌组织形态及纤维化情况;TUNEL染色检测细胞凋亡情况;RT-qPCR检测心肌组织ANP、BNP、β-MHC mRNA表达情况;Western blot检测心肌组织NLRP3、ASC、caspase-1、cleavedcaspase-1、GSDMD和GSDMD-NT蛋白表达情况。结果DJC能减轻db/db小鼠心肌病理损伤,减少胶原沉积和细胞凋亡,降低血糖、血脂、心肌酶和炎症因子水平,降低心肌组织中ANP、BNP、β-MHC mRNA表达,以及NLRP3、ASC、caspase-1、cleavedcaspase-1、GSDMD、GSDMD-NT蛋白表达。结论DJC可减轻db/db小鼠心肌损伤,其可能是通过抑制NLRP3炎症小体的激活,减轻心肌细胞焦亡,改善炎症状态来发挥作用。 展开更多
关键词 丹蛭降糖胶囊 糖尿病心肌病 db/db小鼠 NLRP3炎症小体 细胞焦亡 心肌损伤
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Canadian Agility and Movement Skill Assessment(CAMSA):Validity, objectivity, and reliability evidence for children 8–12 years of age 被引量:13
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作者 Patricia E.Longmuir Charles Boyer +6 位作者 Meghann Lloyd Michael M.Borghese Emily Knight Travis J.Saunders Elena Boiarskaia Weimo Zhu Mark S.Tremblay 《Journal of Sport and Health Science》 SCIE 2017年第2期231-240,共10页
Purpose: The primary aim of this study was to develop an assessment of the fundamental, combined, and complex movement skills required to support childhood physical literacy. The secondary aim was to establish the fea... Purpose: The primary aim of this study was to develop an assessment of the fundamental, combined, and complex movement skills required to support childhood physical literacy. The secondary aim was to establish the feasibility, objectivity, and reliability evidence for the assessment.Methods: An expert advisory group recommended a course format for the assessment that would require children to complete a series of dynamic movement skills. Criterion-referenced skill performance and completion time were the recommended forms of evaluation. Children, 8–12 years of age, self-reported their age and gender and then completed the study assessments while attending local schools or day camps. Face validity was previously established through a Delphi expert(n = 19, 21% female) review process. Convergent validity was evaluated by age and gender associations with assessment performance. Inter-and intra-rater(n = 53, 34% female) objectivity and test–retest(n = 60, 47% female) reliability were assessed through repeated test administration.Results: Median total score was 21 of 28 points(range 5–28). Median completion time was 17 s. Total scores were feasible for all 995 children who self-reported age and gender. Total score did not differ between inside and outside environments(95% confidence interval(CI) of difference:-0.7 to 0.6;p = 0.91) or with/without footwear(95%CI of difference:-2.5 to 1.9; p = 0.77). Older age(p < 0.001, η2= 0.15) and male gender(p < 0.001, η2= 0.02)were associated with a higher total score. Inter-rater objectivity evidence was excellent(intraclass correlation coefficient(ICC) = 0.99) for completion time and substantial for skill score(ICC = 0.69) for 104 attempts by 53 children(34% female). Intra-rater objectivity was moderate(ICC = 0.52) for skill score and excellent for completion time(ICC = 0.99). Reliability was excellent for completion time over a short(2–4 days; ICC = 0.84) or long(8–14days; ICC = 0.82) interval. Skill score reliability was moderate(ICC = 0.46) over a short interval, and substantial(ICC = 0.74) over a long interval.Conclusion: The Canadian Agility and Movement Skill Assessment is a feasible measure of selected fundamental, complex and combined movement skills, which are an important building block for childhood physical literacy. Moderate-to-excellent objectivity was demonstrated for children 8–12 years of age. Test–retest reliability has been established over an interval of at least 1 week. The time and skill scores can be accurately estimated by 1 trained examiner. 展开更多
关键词 Agility course CHILDREN Dynamic motor skill Locomotor skill object manipulation Population assessment
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DB^(R)_(π)矩阵的线性互补问题的误差界
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作者 李艳艳 《鞍山师范学院学报》 2025年第4期1-7,共7页
为了研究DB^(R)_(π)矩阵H的线性互补问题的误差界,首先基于DB^(R)_(π)矩阵的结构特性,将其分解为两个矩阵之和B^(+)+C,其中B+是主对角元素为正的双严格对角占优矩阵,再结合矩阵H的[H^(-1)]∽和两个特殊不等式,得到max_(d∈[0,1]^(n))[... 为了研究DB^(R)_(π)矩阵H的线性互补问题的误差界,首先基于DB^(R)_(π)矩阵的结构特性,将其分解为两个矩阵之和B^(+)+C,其中B+是主对角元素为正的双严格对角占优矩阵,再结合矩阵H的[H^(-1)]∽和两个特殊不等式,得到max_(d∈[0,1]^(n))[(I-D+DH)^(-1)]∽的误差界. 展开更多
关键词 db^(R)_(π)矩阵 线性互补 误差界
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模式动物db/db和KK-Ay作为糖尿病肾病模型的特点及差异性研究
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作者 陈群英 周波 +1 位作者 刘晶 侯艳 《中国比较医学杂志》 北大核心 2025年第5期71-80,共10页
目的考察两种2型糖尿病模式鼠db/db和KK-Ay作为糖尿病肾病(diabetic nephropathy,DN)模型的特点及差异性,并用厄贝沙坦(irbesartan)治疗验证。方法12周龄的db/db及KK-Ay鼠各102只,根据尿微量白-蛋白肌酐比值(albumin-to-creatinine rati... 目的考察两种2型糖尿病模式鼠db/db和KK-Ay作为糖尿病肾病(diabetic nephropathy,DN)模型的特点及差异性,并用厄贝沙坦(irbesartan)治疗验证。方法12周龄的db/db及KK-Ay鼠各102只,根据尿微量白-蛋白肌酐比值(albumin-to-creatinine ratio,ACR)、24 h尿总蛋白(UCFP)每种模式鼠分别筛选出78只,分为6组,db/db+irbesartan组和KK-Ay+irbesartan组分别给予irbesartan(40 mg/kg,)、db/db组和KK-Ay组分别给予等量实验动物饮用水,以及db/db+受试物A组和KK-Ay+受试物A组(给予不同剂量受试物A,本文受试物A组仅展示死亡动物数结果,其他结果未展示)。野生型C57BL/6J(WTa/WTb组)作为正常对照组,给予实验动物饮用水。每天临床观察,每周称体质量,不定期监测ACR、UCFP。给药结束解剖,取脑、肝、肺、肾,称体质量,计算脏器系数(脏器质量×1000/动物体质量),采血检测血清总蛋白(totla protein,TP)、白蛋白(albumin,Alb)、血尿素氮(blood urea nitrogen,BUN)、葡萄糖(glucose,GLU)、甘油三酯(triglycerides,TG)、胆固醇(cholesterol,CHO)。肾组织HE、MASSON和PAS染色观察组织病理学改变。结果78只KK-Ay鼠8周共死亡5只,78只db/db鼠6周共死亡21只;ACR在db/db组、KK-Ay组均随鼠龄增加而升高,在db/db+irbesartan组和KK-Ay+irbesartan组降低但无统计学意义(P>0.05);UCFP在KK-Ay组随鼠龄增加而增大,irbesartan治疗后显著降低(P<0.05);与WT组相比,db/db组、KK-Ay组肾系数无明显变化,脑系数显著降低(P<0.01),肝系数显著升高(P<0.01),irbesartan治疗后无显著变化(P>0.05);与WT组相比,db/db组、KK-Ay组小鼠的血清GLU、TG显著升高(P<0.01),与KK-Ay组相比,KK-Ay+irbesartan组GLU、TG显著降低(P<0.01);db/db组、KK-Ay组小鼠肾均可见肾小球系膜基质增厚伴系膜细胞增生,irbesartan治疗后病变程度减轻。结论12周龄的db/db、KK-Ay小鼠均可作为DN研究模型。相比db/db小鼠,KK-Ay小鼠的ACR个体差异小,采尿后动物死亡少,irbesartan治疗后GLU、TG、UCFP均有显著改善。 展开更多
关键词 糖尿病肾病 实验动物模型 db/db KK-AY ACR UCFP MAU
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裕丹参提取物对db/db小鼠认知障碍及脑损伤的影响
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作者 叶凯利 曾梦楠 +5 位作者 曹兵 郝凤霄 郭彭莉 张梓玉 冯卫生 郑晓珂 《中国新药杂志》 北大核心 2025年第11期1219-1227,共9页
目的:研究裕丹参提取物对db/db小鼠认知障碍及脑损伤的影响,为裕丹参临床用药提供实验依据。方法:雄性db/m小鼠作为对照组,同时雄性db/db糖尿病小鼠随机分为模型组、桑枝总生物碱组(24 mg·kg^(-1))、二甲双胍组(160 mg·kg^(-... 目的:研究裕丹参提取物对db/db小鼠认知障碍及脑损伤的影响,为裕丹参临床用药提供实验依据。方法:雄性db/m小鼠作为对照组,同时雄性db/db糖尿病小鼠随机分为模型组、桑枝总生物碱组(24 mg·kg^(-1))、二甲双胍组(160 mg·kg^(-1))及裕丹参提取物低剂量(24.58 mg·kg^(-1))、高剂量组(49.16 mg·kg^(-1)),灌胃给药4周,每周检测空腹血糖。给药结束后,生化法检测各组小鼠胰岛素抵抗、胰腺损伤、学习记忆功能、神经元损伤、脑组织纤维化相关指标;蛋白印迹法检测IRS-1/PI3K/AKT通路相关蛋白水平;利用MTT法检测裕丹参提取物对高糖诱导N9细胞损伤的影响。结果:裕丹参提取物可以降低db/db小鼠的空腹血糖及胰岛素抵抗指数、回调胰岛β细胞/胰岛α细胞比值、恢复胰岛形态;此外,裕丹参提取物可改善db/db小鼠的学习记忆功能,减少小鼠海马神经元损伤和脑内胶原沉积,并可显著激活IRS-1/PI3K/AKT通路;裕丹参提取物能够恢复高糖诱导N9细胞的细胞活力和形态。结论:裕丹参提取物可以有效改善db/db小鼠的认知障碍及脑损伤,且可能与激活IRS-1/PI3K/AKT信号通路有关。 展开更多
关键词 裕丹参提取物 db/db小鼠 糖尿病 脑损伤
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中药复方汉唐平通过调控巨噬细胞极化与Gal-3/TLR4/NF-κB信号通路改善db/db肥胖小鼠脂肪组织慢性炎症
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作者 张钰莹 袁颢瑜 +2 位作者 黄薇宇 王保华 李赛美 《中药新药与临床药理》 北大核心 2025年第10期1636-1645,共10页
目的基于巨噬细胞极化与半乳糖凝集素3(Gal-3)/Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路探究中药复方汉唐平改善db/db肥胖小鼠脂肪组织慢性炎症的作用机制。方法将40只db/db小鼠随机分为模型组、二甲双胍组(30 mg·kg^(-1))、... 目的基于巨噬细胞极化与半乳糖凝集素3(Gal-3)/Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路探究中药复方汉唐平改善db/db肥胖小鼠脂肪组织慢性炎症的作用机制。方法将40只db/db小鼠随机分为模型组、二甲双胍组(30 mg·kg^(-1))、汉唐平组(50.6 g·kg^(-1))、GB1107组(30 mg·kg^(-1)),每组10只;另外选取10只C57BL/6J小鼠作为对照组;各组均按照10 mL·kg^(-1)给药体积进行灌胃,每日1次,连续12周;对照组、模型组给予等量蒸馏水灌胃。给药期间每2周称量并记录小鼠体质量,取材后称取各组小鼠双侧附睾脂肪组织质量并记录;采用全自动生化仪检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平;ELISA法检测血清肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、Gal-3水平;HE染色法观察附睾脂肪组织病理变化;免疫组化法检测附睾脂肪组织巨噬细胞标记物分化簇68(CD68)表达情况;免疫荧光法检测附睾脂肪组织巨噬细胞极化标记物分化簇86(CD86)、分化簇206(CD206)表达情况;RT-qPCR法检测附睾脂肪组织促炎因子TNF-α、IL-1β、IL-6及抗炎因子白细胞介素10(IL-10)mRNA表达水平;Western Blot法检测附睾脂肪组织Gal-3/TLR4/NF-κB信号通路相关蛋白的表达水平。结果与对照组比较,模型组小鼠的体质量、附睾脂肪组织质量及血清TC、TG、LDL-C、TNF-α、IL-1β、IL-6、Gal-3水平均显著升高(P<0.001);附睾脂肪细胞大小不一,排列紊乱,质地疏松,细胞横截面积显著增大(P<0.001),且脂肪间质内出现大量呈花冠样结构浸润的炎症细胞;附睾脂肪组织的CD68表达水平显著升高(P<0.001),周围大量巨噬细胞浸润,形成典型花冠样结构;附睾脂肪组织CD86表达水平显著升高(P<0.001),CD206表达水平显著降低(P<0.001),M1型巨噬细胞释放的促炎因子TNF-α、IL-1β、IL-6 mRNA表达水平显著升高(P<0.001),而M2型巨噬细胞释放的抑炎因子IL-10 mRNA表达水平显著降低(P<0.001);附睾脂肪组织Gal-3、TLR4、MyD88蛋白表达水平及IκB-α、NF-κB P65蛋白磷酸化水平均显著升高(P<0.001)。与模型组比较,各给药组小鼠体质量、附睾脂肪组织质量及血清TC、TG、LDL-C、TNF-α、IL-1β、IL-6、Gal-3水平均显著降低(P<0.01,P<0.001);附睾脂肪细胞形态均明显改善,大小趋于均等,排列较为有序,细胞横截面积显著减小(P<0.001),同时花冠样结构浸润的炎症细胞显著减少;附睾脂肪组织的巨噬细胞数量减少,CD68表达水平显著降低(P<0.01,P<0.001);附睾脂肪组织CD86表达水平均显著降低(P<0.001),CD206表达水平显著升高(P<0.01,P<0.001),TNF-α、IL-1β、IL-6 mRNA表达水平显著降低(P<0.01,P<0.001),IL-10 mRNA表达水平显著升高(P<0.05,P<0.01);附睾脂肪组织Gal-3、TLR4、MyD88蛋白表达水平及IκB-α、NF-κB P65蛋白磷酸化水平均显著降低(P<0.05,P<0.01,P<0.001)。结论中药复方汉唐平能够有效减轻db/db肥胖小鼠的体质量及脂肪积聚,纠正脂代谢紊乱,抑制附睾脂肪组织中巨噬细胞浸润和M1型极化,降低促炎因子表达水平,从而改善脂肪组织的慢性炎症反应,其作用机制可能与调控Gal-3/TLR4/NF-κB信号通路的活性有关。 展开更多
关键词 汉唐平 肥胖 2型糖尿病 脂肪组织慢性炎症 巨噬细胞极化 Gal-3/TLR4/NF-κB信号通路 db/db小鼠
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ON UNIQUENESS,EXISTENCE AND OBJECTIVITY OF S-R DECOMPOSITION THEOREM
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作者 陈勉 梁景伟 +1 位作者 陈熙 陈至达 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 1997年第9期817-823,共7页
For a physically possible deformation field of a continuum, the deformation gradient function F can be decomposed into direct sum of a symmetric tensor S and on orthogonal tensor R, which is called S-R decomposition t... For a physically possible deformation field of a continuum, the deformation gradient function F can be decomposed into direct sum of a symmetric tensor S and on orthogonal tensor R, which is called S-R decomposition theorem. In this paper, the S-R decomposition unique existence theorem is proved, by employing matrix and tensor method. Also, a brief proof of its objectivity is given. 展开更多
关键词 S-R decomposition theorem UNIQUENESS EXISTENCE objectivity
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益糖康通过PPAR-α/PGC-1α信号通路调节异位脂质沉积改善db/db小鼠肾脏损伤的机制研究
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作者 王诗怡 孙贵炎 +2 位作者 张晖 杨宇峰 石岩 《中国中医药信息杂志》 2025年第12期89-96,共8页
目的 观察益糖康对db/db小鼠肾脏异位脂质沉积及PPAR-α/PGC-1α信号通路的影响,探讨其改善肾脏损伤的作用机制。方法 采用随机数字表法将30只db/db小鼠分为模型组、益糖康组和氯沙坦钾组(每组10只),并设10只db/m小鼠为空白组,分别予相... 目的 观察益糖康对db/db小鼠肾脏异位脂质沉积及PPAR-α/PGC-1α信号通路的影响,探讨其改善肾脏损伤的作用机制。方法 采用随机数字表法将30只db/db小鼠分为模型组、益糖康组和氯沙坦钾组(每组10只),并设10只db/m小鼠为空白组,分别予相应干预8周。检测小鼠体质量、空腹血糖(FBG)、血肌酐(SCr)、血尿素氮(BUN)、24 h尿蛋白含量,HE、PAS、Masson染色观察肾组织形态及损伤程度,油红O染色观察小鼠肾脏脂质沉积情况,透射电镜观察足细胞超微结构,免疫荧光染色检测肾组织肉碱棕榈酰转移酶1A(CPT1A)、核因子E2相关因子2(Nrf2)表达,实时荧光定量PCR检测过氧化物酶体增殖物激活受体α(PPAR-α)、过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)m RNA表达,Western blot检测PPAR-α、CPT1A、PGC-1α、Nrf2、核呼吸因子1(NRF1)、线粒体转录因子A(TFAM)蛋白表达。结果 与空白组比较,模型组小鼠体质量、FBG显著升高(P<0.05),SCr、BUN、24 h尿蛋白含量显著增加(P<0.05);肾小球肥大,系膜细胞与基质增生,基底膜增厚,肾间质胶原纤维异常沉积,肾组织脂质沉积程度增加,足细胞足突广泛融合,足突密度显著降低(P<0.05),线粒体外膜边界模糊,形态肿胀,内嵴模糊不清;肾组织PPAR-α、PGC-1α mRNA表达显著降低(P<0.05),PPAR-α、CPT1A、PGC-1α、Nrf2、NRF1、TFAM蛋白表达显著降低(P<0.05)。与模型组比较,益糖康组小鼠体质量和FBG显著降低(P<0.05),氯沙坦钾组上述指标差异无统计学意义(P>0.05);益糖康组和氯沙坦钾组SCr、BUN、24 h尿蛋白含量显著降低(P<0.05);肾组织病理损伤减轻、脂质沉积减少,足细胞超微结构改善,足突密度显著升高(P<0.05);肾组织PPAR-α、PGC-1α mRNA表达升高,PPAR-α、CPT1A、PGC-1α、Nrf2、NRF1、TFAM蛋白表达升高,差异均有统计学意义(P<0.05)。结论 益糖康能有效改善糖尿病肾病小鼠肾脏损伤,其机制可能与激活PPAR-α/PGC-1α信号通路,减少肾脏脂质沉积和线粒体生物发生有关。 展开更多
关键词 益糖康 2型糖尿病 脂质沉积 线粒体生物发生 PPAR-α/PGC-1α信号通路 db/db小鼠
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Somewhere This Side of the View from Nowhere: On the Phenomenological Prepredicative Grounding of the Idea of Objectivity
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作者 Andrea Altobrando 《学术界》 CSSCI 北大核心 2019年第1期197-211,共15页
Although objectivity is mainly accounted for in terms of linguistic thought and communication,in this article I will aim to showthat at least one condition of possibility for our understanding of objectivity is ground... Although objectivity is mainly accounted for in terms of linguistic thought and communication,in this article I will aim to showthat at least one condition of possibility for our understanding of objectivity is grounded on a prepredicative,i. e. pre-linguistic and pre-communicative,level. I will endorse a Husserlian viewpoint on the issue,and I will try to develop some aspects of the Husserlian account of three-dimensional thing-perception by means of which I will showhowprepredicative experience can actually offer us a fundamental element of our common understanding of objectivity. In doing this,it will be necessary to acknowledge thing-perception as being primarily intertwined with indeterminacy. I will claim that only on the basis of such an intuitive and prepredicative access to the things as partially indeterminate,first,and as determinable,second,is it possible to have an understanding of the world as something (at least partially) independent from the intuition (s) all subjects can have of it. By means of the addition of a consciousness of the thing as accessible to other subjects,one achieves a vision of the thing as fully determinate in itself. This"vision",however,takes one to be aware of the determination of the thing as lying beyond any intuitive grasp of it. The result will,thus,be that the prepredicative constitution of our basic sense of objectivity leads us to intend the world as something which should be accounted for (also) by means of sources different from intuition. 展开更多
关键词 objectivity thing-perception prepredicative experience INDETERMINACY Husserlian EPISTEMOLOGY
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基于Nrf2/Keap1/ARE信号通路探讨芪蛭通络方对db/db小鼠肾损伤的影响
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作者 杨冰 张尧 +4 位作者 毛竞宇 唐淼 杨凤文 吴红红 檀金川 《中成药》 北大核心 2025年第1期73-80,共8页
目的探讨芪蛭通络方对糖尿病肾病小鼠的肾脏保护作用。方法雄性db/db小鼠随机分为模型组、达格列净组(0.76 mg/kg)和芪蛭通络方低、中、高剂量组(7.83、15.65、31.3 g/kg),每组6只,6只db/m小鼠作为对照组,对照组和模型组小鼠灌胃给予蒸... 目的探讨芪蛭通络方对糖尿病肾病小鼠的肾脏保护作用。方法雄性db/db小鼠随机分为模型组、达格列净组(0.76 mg/kg)和芪蛭通络方低、中、高剂量组(7.83、15.65、31.3 g/kg),每组6只,6只db/m小鼠作为对照组,对照组和模型组小鼠灌胃给予蒸馏水,各给药组灌胃给予相应剂量药物,每天1次,连续8周。给药结束后,检测小鼠FBG、BUN、Scr、24 h-UTP水平,透射电镜和HE染色观察肾组织病理变化,ELISA法检测小鼠血清Nrf2、HO-1、Keap1水平和肾组织氧化应激水平,免疫荧光(IF)法观察肾组织Nrf2蛋白表达,Western blot法检测肾组织Nrf2、HO-1、Keap1蛋白表达,RT-qPCR法检测肾组织Nrf2、HO-1、Keap 1 mRNA表达。结果与对照组比较,模型组小鼠24 h-UTP、Scr、FBG和肾组织MDA水平升高(P<0.01),肾组织SOD、CAT、GSH-Px活性降低(P<0.01),肾小球轻度系膜增生,肾小管上皮细胞空泡变性,足细胞足突广泛融合,裂孔膜消失,血清Nrf2、HO-1分泌水平和肾组织Nrf2、HO-1蛋白、mRNA表达降低(P<0.05,P<0.01),血清Keap1分泌水平和肾组织Keap1蛋白、mRNA表达升高(P<0.01);与模型组比较,芪蛭通络方高剂量组小鼠24 h-UTP、Scr、FBG和肾组织MDA水平降低(P<0.01),肾组织SOD、CAT、GSH-Px活性升高(P<0.01),肾组织病理性损伤减轻,血清Nrf2、HO-1分泌水平和肾组织Nrf2、HO-1蛋白、mRNA表达升高(P<0.01),血清Keap1分泌水平和肾组织Keap1蛋白、mRNA表达降低(P<0.01)。结论芪蛭通络方可通过激活Nrf2/Keap1/ARE信号通路来抑制氧化应激,减轻db/db小鼠肾脏损伤。 展开更多
关键词 芪蛭通络方 糖尿病肾脏病 肾损伤 db/db小鼠 氧化应激 Nrf2/Keap1/ARE信号通路
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益肾化湿颗粒通过p38 MAPK信号通路对db/db小鼠肾脏保护机制
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作者 周楷栋 王思童 +6 位作者 靳鸽 蔡炎沫 周鑫 刘运华 张新雪 张敏 赵宗江 《中国实验方剂学杂志》 北大核心 2025年第3期58-68,共11页
目的:观察益肾化湿颗粒对丝裂原活化蛋白激酶(MAPK)信号通路的影响,减轻肾小管上皮细胞损伤,延缓糖尿病肾病的作用机制。方法:12周龄db/db小鼠随机分为模型组、达格列净组(1.6 mg·kg^(-1))和益肾化湿颗粒组(4.7 g·kg^(-1)),d... 目的:观察益肾化湿颗粒对丝裂原活化蛋白激酶(MAPK)信号通路的影响,减轻肾小管上皮细胞损伤,延缓糖尿病肾病的作用机制。方法:12周龄db/db小鼠随机分为模型组、达格列净组(1.6 mg·kg^(-1))和益肾化湿颗粒组(4.7 g·kg^(-1)),db/m小鼠为正常组。观察小鼠一般状况,给药前和第12周检测空腹血糖、24 h尿蛋白定量和尿白蛋白/尿肌酐(ACR);给药12周后取材,收集血清检测血肌酐(SCr)、血尿素(UREA)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)指标;采用苏木素-伊红(HE)染色法、过碘酸雪夫(PAS)染色法、马洛里(Mallory)三色染色法和透射电镜观察肾组织病理形态;实时荧光定量聚合酶链式反应(Real-time PCR)检测小鼠肾组织单核细胞趋化蛋白-1(MCP-1)、CC基序趋化因子受体2(CCR2)mRNA水平;免疫组化检测小鼠肾组织p38蛋白激酶(p38)、磷酸化(p)-p38、MCP-1、CCR2蛋白表达,蛋白免疫印迹法(Western blot)检测小鼠肾组织p38、p-p38、MCP-1、CCR2蛋白水平。体外培养HK-2细胞分为空白组、高糖组(30 mmol·L^(-1))、益肾化湿含药血清组及SB203580组,各组细胞培养48 h后,提取细胞RNA和蛋白,通过Real-time PCR检测MCP-1、CCR2 mRNA水平,通过Western blot检测p38、p-p38、MCP-1、CCR2蛋白水平。结果:体内实验中,给药前与正常组比较,各组db/db小鼠体质量显著升高、血糖、24 h尿蛋白定量、ACR均显著升高(P<0.01);给药12周后,与模型组比较,益肾化湿颗粒组一般状态明显改善,体质量有下降趋势,血糖、24 h尿蛋白定量、ACR均明显下降(P<0.05,P<0.01),SCr和UREA显著降低(P<0.01),TC、TG、LDL水平明显降低(P<0.05,P<0.01)。与模型组比较,益肾化湿颗粒组病理染色显示肾组织损伤及肾间质纤维化水平明显改善,电镜观察发现肾小球足突融合、基底膜增厚情况减轻,PCR显示肾组织MCP-1、CCR2 mRNA表达显著降低(P<0.01),Western blot显示肾组织p-p38、MCP-1、CCR2蛋白表达显著降低(P<0.01),肾组织p-p38/p38、MCP-1、CCR2表达明显降低(P<0.05)。体外实验中,与高糖组比较,益肾化湿颗粒含药血清组MCP-1、CCR2 mRNA表达显著降低(P<0.01),p-p38/p38、MCP-1、CCR2蛋白表达明显降低(P<0.05,P<0.01)。结论:益肾化湿颗粒可以调节db/db小鼠糖脂代谢,降低24 h尿蛋白和尿ACR,改善肾功能,并能减轻高糖导致的肾小管损伤,对肾小管上皮细胞有保护作用,其机制可能是通过p38 MAPK信号通路,减少MCP-1/CCR2激活而实现的。 展开更多
关键词 糖尿病肾病 益肾化湿颗粒 db/db小鼠 p38丝裂原活化蛋白激酶(p38 MAPK)信号通路 肾痿
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基于16S rRNA测序的2型糖尿病db/db小鼠模型口腔菌群差异分析
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作者 潘钱家 葛峻沂 +2 位作者 胡楠 华飞 顾敏 《实验动物与比较医学》 2025年第2期147-157,共11页
目的研究db/db小鼠口腔菌群的改变,为探寻2型糖尿病与口腔微生态的联系提供实验基础。方法选取8只10周龄雄性db/db小鼠为糖尿病实验组,8只10周龄雄性db/m小鼠为正常对照组。适应性喂养5 d后,在相同饲料饲养的第6天和第37天行尾静脉采血... 目的研究db/db小鼠口腔菌群的改变,为探寻2型糖尿病与口腔微生态的联系提供实验基础。方法选取8只10周龄雄性db/db小鼠为糖尿病实验组,8只10周龄雄性db/m小鼠为正常对照组。适应性喂养5 d后,在相同饲料饲养的第6天和第37天行尾静脉采血,测定两组小鼠的空腹血糖(fasting blood glucose,FBG)水平和口服葡萄糖耐量(oral glucose tolerance test,OGTT),以验证糖尿病模型的可靠性。在相同饲料饲养的第15天采集两组小鼠两侧颊黏膜、舌背与舌腹、口底黏膜、上腭硬区黏膜、上下颌牙龈的龈上区域的口腔菌群样本,提取口腔微生物的基因组DNA后,使用GeneAmp 9700热循环仪对其中16S核糖体RNA(16S ribosomal RNA,16S rRNA)基因的V3~V4区进行扩增,然后在Illumina MiSeq平台上通过双标引扩增和测序对口腔菌群结构进行评估,然后应用QIIME(version 1.6.0)软件进行生物信息学分析。结果实验开始后第6天和第37天的FBG水平和OGTT检测结果均显示db/db小鼠比db/m小鼠表现出明显的2型糖尿病症状。α多样性分析显示两组小鼠的口腔菌群在多样性上无显著差异(P>0.05),而在丰富度上差异显著(P<0.05)。主坐标分析显示db/db小鼠的口腔菌群结构与db/m小鼠存在显著差异(P<0.05),物种组成分析以及LEfSe分析显示db/db小鼠口腔菌群在门水平以变形菌门(p_Proteobacteria)为主的相对丰度显著上升(P<0.05),而在属水平上变形杆菌属(g_Proteus)和肠球菌属(g_Enterococcus)的相对丰度极显著上升(P<0.001)。结论2型糖尿病db/db小鼠的口腔菌群结构和丰富度均较血糖正常的对照小鼠出现了显著变化。 展开更多
关键词 2型糖尿病 db/db小鼠 口腔菌群 16S rRNA测序
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带泊松跳的DB养老金计划中鲁棒均衡策略
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作者 公雪 赵永霞 《应用概率统计》 北大核心 2025年第5期698-713,共16页
研究了一个带有泊松跳跃资金过剩确定收益养老金计划中的鲁棒非合作博弈问题.公司管理者关心基金盈余的投资业绩,养老金参与者要求分享基金盈余.金融市场中风险资产的价格由带泊松跳的扩散过程来刻画,并且金融市场中的概率模型是不确定... 研究了一个带有泊松跳跃资金过剩确定收益养老金计划中的鲁棒非合作博弈问题.公司管理者关心基金盈余的投资业绩,养老金参与者要求分享基金盈余.金融市场中风险资产的价格由带泊松跳的扩散过程来刻画,并且金融市场中的概率模型是不确定的.假设公司管理者和养老金参与者是模糊厌恶的.基于鲁棒控制策略,养老金参与者的目标是最大化收益的预期贴现效用,公司的目标是寻求基金盈余效用的最大化.利用鲁棒控制理论得到了非合作博弈下的鲁棒均衡投资和支付策略.最后使用数值结果解释了博弈中鲁棒策略的经济行为. 展开更多
关键词 非合作博弈 db养老金计划 模糊厌恶 均衡策略 泊松跳跃
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