The most important optical component in an optical fiber endoscope is its objective lens.To achieve a high imaging performance level,the development of an ultra-compact objective lens is thus the key to an ultra-thin ...The most important optical component in an optical fiber endoscope is its objective lens.To achieve a high imaging performance level,the development of an ultra-compact objective lens is thus the key to an ultra-thin optical fiber endoscope.In this work,we use femtosecond laser 3D printing to develop a series of micro objective lenses with different optical designs.The imaging resolution and field-of-view performances of these printed micro objective lenses are investigated via both simulations and experiments.For the first time,multiple micro objective lenses with different fields of view are printed on the end face of a single imaging optical fiber,thus realizing the perfect integration of an optical fiber and objective lenses.This work demonstrates the considerable potential of femtosecond laser 3D printing in the fabrication of micro-optical systems and provides a reliable solution for the development of an ultrathin fiber endoscope.展开更多
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously anal...Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.展开更多
Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework...Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework employs specially designed large-scale objective lenses to correct aberrations across extended FOVs,synchronized with light-field acquisition modalities through either scanning point detection or large-format array detection.Conventional microscopes,constrained by the limitations of objective lenses,exhibit a trade-off between the FOV and resolution.To achieve both high resolution and a large FOV,common approaches such as FOV stitching and Fourier ptychography were employed.However,these methods were extremely slow and imposed numerous constraints on samples.In 2016,a mesoscopic objective lens was introduced to address these challenges,achieving a 6 mm FOV and 0.7 mm resolution,thereby increasing the imaging throughput of conventional objective lenses by orders of magnitude.1 In the same year,this technology was recognized as one of the top ten physics breakthroughs worldwide by Physics World.Since then,mesoscopic imaging technology has gradually gained momentum and has been applied in various fields.展开更多
基金This work was supported by Shenzhen Science and Technology Program(RCYX20200714114524139,Shenzhen Key Laboratory of Ultrafast Laser Micro/Nano Manufacturing ZDSYS20220606100405013)Natural Science Foundation of Guangdong Province(2022B1515120061)National Natural Science Foundation of China(62122057,62075136).
文摘The most important optical component in an optical fiber endoscope is its objective lens.To achieve a high imaging performance level,the development of an ultra-compact objective lens is thus the key to an ultra-thin optical fiber endoscope.In this work,we use femtosecond laser 3D printing to develop a series of micro objective lenses with different optical designs.The imaging resolution and field-of-view performances of these printed micro objective lenses are investigated via both simulations and experiments.For the first time,multiple micro objective lenses with different fields of view are printed on the end face of a single imaging optical fiber,thus realizing the perfect integration of an optical fiber and objective lenses.This work demonstrates the considerable potential of femtosecond laser 3D printing in the fabrication of micro-optical systems and provides a reliable solution for the development of an ultrathin fiber endoscope.
基金supported by National Key R&D Program of China(grant no.2022YFC2404201)the Chinese Academy of Sciences Project for Young Scientists in Basic Research(grant no.YSBR067).
文摘Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.
基金supported by the Chinese Academy of Sciences Project for Young Scientists in Basic Research(YSBR067)the Natural Science Foundation of Jiangsu Province(BK20240024)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Y2023087)。
文摘Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework employs specially designed large-scale objective lenses to correct aberrations across extended FOVs,synchronized with light-field acquisition modalities through either scanning point detection or large-format array detection.Conventional microscopes,constrained by the limitations of objective lenses,exhibit a trade-off between the FOV and resolution.To achieve both high resolution and a large FOV,common approaches such as FOV stitching and Fourier ptychography were employed.However,these methods were extremely slow and imposed numerous constraints on samples.In 2016,a mesoscopic objective lens was introduced to address these challenges,achieving a 6 mm FOV and 0.7 mm resolution,thereby increasing the imaging throughput of conventional objective lenses by orders of magnitude.1 In the same year,this technology was recognized as one of the top ten physics breakthroughs worldwide by Physics World.Since then,mesoscopic imaging technology has gradually gained momentum and has been applied in various fields.
