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Predicting Nonlinear Wave Trough Distributions Utilizing A Transformed Linear Simulation Method
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作者 WANG Ying-guang 《China Ocean Engineering》 SCIE EI CSCD 2018年第6期655-664,共10页
This paper first proposes a new approach for predicting the nonlinear wave trough distributions by utilizing a transformed linear simulation method. The linear simulation method is transformed based on a Hermite trans... This paper first proposes a new approach for predicting the nonlinear wave trough distributions by utilizing a transformed linear simulation method. The linear simulation method is transformed based on a Hermite transformation model where the transformation is chosen to be a monotonic cubic polynomial and calibrated such that the first four moments of the transformed model match the moments of the true process. The proposed new approach is applied for calculating the wave trough distributions of a nonlinear sea state with the surface elevation data measured at the coast of Yura in the Japan Sea, and its accuracy and efficiency are convincingly validated by comparisons with the results from two theoretical distribution models, from a linear simulation model and a secondorder nonlinear simulation model. Finally, it is further demonstrated in this paper that the new approach can be applied to all the situations characterized by similar nondimensional spectrum. 展开更多
关键词 nonlinear wave troughs transformed linear simulation Hermite transformation nonlinear simulation
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Numerical Simulation of Nonlinear Sloshing in a Prismatic Tank Under Combined Surge and Roll Excitations
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作者 ZHANG Yun-xing ZHANG Yi-fan 《China Ocean Engineering》 2025年第6期1070-1082,共13页
This paper presents a numerical investigation of nonlinear sloshing in a prismatic tank.A three-dimensional,twophase flow model based on Cartesian grid is developed to simulate the phenomenon.The model solves incompre... This paper presents a numerical investigation of nonlinear sloshing in a prismatic tank.A three-dimensional,twophase flow model based on Cartesian grid is developed to simulate the phenomenon.The model solves incompressible Navier-Stokes equations,utilizing the fractional step method for velocity-pressure decoupling.The finite difference method discretizes spatial derivatives,with specific schemes implemented to enhance model robustness.Model validation involves simulating benchmark cases,and comparing wave profiles and pressure results with published experimental data and numerical findings.The model demonstrates robustness and accuracy in simulating violent sloshing.The validated model examines sloshing in a partially filled prismatic tank under combined surge and roll excitations.The study employs eight frequencies encompassing the natural frequencies of tank roll and surge motions.Roll motion excitation is fixed at 2°,while surge motion considers three excitation amplitudes(0.0 m,0.01 m,and 0.02m).Analysis reveals the effects of surge amplitude and excitation frequency on wave patterns,amplitudes,and pressure peaks.Results indicate the presence of multi-component waves,including transverse,diagonal,and longitudinal waves.Furthermore,the findings demonstrate a reduction in the natural frequency for surge motion through pressure peak analysis. 展开更多
关键词 nonlinear sloshing Cartesian grid prismatic tank combined excitations
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Numerical Simulation of Nonlinear Three-Dimensional Waves in Water of Arbitrary Varying Topography 被引量:10
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作者 Hong, Guangwen Zhang, Hongsheng Feng, Weibing 《China Ocean Engineering》 SCIE EI 1998年第4期383-404,共22页
The numerical simulation is based on the authors' high-order models with a dissipative term for nonlinear and dispersive wave in water of varying depth. Corresponding finite-difference equations and general condit... The numerical simulation is based on the authors' high-order models with a dissipative term for nonlinear and dispersive wave in water of varying depth. Corresponding finite-difference equations and general conditions for open and fixed natural boundaries with an arbitrary reflection coefficient and phase shift are also given in this paper. The systematical tests of numerical simulation show that the theoretical models, the finite-difference algorithms and the boundary conditions can give good calculation results for the wave propagating in shallow and deep water with an arbitrary slope varying from gentle to steep. 展开更多
关键词 numerical simulation nonlinear 3D waves boundary conditions
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2-D Composite Model for Numerical Simulations of Nonlinear Waves 被引量:7
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作者 齐鹏 王永学 邹志利 《China Ocean Engineering》 SCIE EI 2000年第1期113-120,共8页
A composite model, which is the combination of Boussinesq equations and Volume of Fluid (VOF) method, has been developed for 2-D time-domain computations of nonlinear waves in a large region. The whole computational r... A composite model, which is the combination of Boussinesq equations and Volume of Fluid (VOF) method, has been developed for 2-D time-domain computations of nonlinear waves in a large region. The whole computational region Omega is divided into two subregions. In the near-field around a structure, Omega(2), the flow is governed by 2-D Reynolds Averaged Navier-Stokes equations with a turbulence closure model of k-epsilon equations and numerically solved by the improved VOF method; whereas in the subregion Omega(1) (Omega(1) = Omega - Omega(2)) the flow is governed by one-D Boussinesq equations and numerically solved with the predictor-corrector algorithm. The velocity and the wave surface elevation are matched on the common boundary of the two subregions. Numerical tests have been conducted for the case of wave propagation and interaction with a wave barrier. It is shown that the composite model can help perform efficient computation of nonlinear waves in a large region with the complicated flow fields near structures taken into account. 展开更多
关键词 composite model nonlinear waves Boussinesq equations VOF method numerical simulation
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Fast evaluation of ship-bridge collision force based on nonlinear numerical simulation 被引量:3
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作者 HUZhi-qiang GUYong-ning GAOZhen LIYa-ning 《Journal of Marine Science and Application》 2005年第1期8-14,共7页
The max collision force of ship-bridge collision is one of the most importantreferences for bridge design. By mean linear digital simulation method, the collision forces ofthe collisions between rigid bridge pier and ... The max collision force of ship-bridge collision is one of the most importantreferences for bridge design. By mean linear digital simulation method, the collision forces ofthe collisions between rigid bridge pier and ship bow were calculated out for four different ships,whose tonnages are 5 000,10 000,50 000 and 60 000 DWT respectively. Curves of collisionforce-penetration and absorbed energy-penetration are obtained, and the data of the max loads arethen summarized. On the basis of these curves and data, a set of curves describing therelationships between max collision forces and tonnages of the ships are successfully presented, bywhich the max collision forces of the ships-bridge with different tonnages and in differentvelocities can be estimated easily and reliably. 展开更多
关键词 ship-bridge collision collision force nonlinear digital simulation PENETRATION absorbed energy
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Nonlinear flow numerical simulation of low-permeability reservoir 被引量:2
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作者 于荣泽 卞亚南 +3 位作者 周舒 王楷军 吕琦 陈朝辉 《Journal of Central South University》 SCIE EI CAS 2012年第7期1980-1987,共8页
A nonlinear flow reservoir mathematical model was established based on the flow characteristic of low-permeability reservoir.The well-grid equations were deduced and the dimensionless permeability coefficient was intr... A nonlinear flow reservoir mathematical model was established based on the flow characteristic of low-permeability reservoir.The well-grid equations were deduced and the dimensionless permeability coefficient was introduced to describe the permeability variation of nonlinear flow.The nonlinear flow numerical simulation program was compiled based on black-oil model.A quarter of five-spot well unit was simulated to study the effect of nonlinear flow on the exploitation of low-permeability reservoir.The comprehensive comparison and analysis of the simulation results of Darcy flow,quasi-linear flow and nonlinear flow were provided.The dimensionless permeability coefficient distribution was gained to describe the nonlinear flow degree.The result shows that compared with the results of Darcy flow,when considering nonlinear flow,the oil production is low,and production decline is rapid.The fluid flow in reservoir consumes more driving energy,which reduces the water flooding efficiency.Darcy flow model overstates the reservoir flow capability,and quasi-linear flow model overstates the reservoir flow resistance.The flow ability of the formation near the well and artificial fracture is strong while the flow ability of the formation far away from the main streamline is weak.The nonlinear flow area is much larger than that of quasi-linear flow during the fluid flow in low-permeability reservoir.The water propelling speed of nonlinear flow is greatly slower than that of Darcy flow in the vertical direction of artificial fracture,and the nonlinear flow should be taken into account in the well pattern arrangement of low-permeability reservoir. 展开更多
关键词 low-permeability reservoir nonlinear flow mathematical model numerical simulation
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Fully Nonlinear Simulation for Fluid/Structure Impact:A Review 被引量:2
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作者 Shili Sun Guoxiong Wu 《Journal of Marine Science and Application》 2014年第3期237-244,共8页
This paper presents a review of the work on fluid/structure impact based on inviscid and imcompressible liquid and irrotational flow. The focus is on the velocity potential theory together with boundary element method... This paper presents a review of the work on fluid/structure impact based on inviscid and imcompressible liquid and irrotational flow. The focus is on the velocity potential theory together with boundary element method (BEM). Fully nonlinear boundary conditions are imposed on the unknown free surface and the wetted surface of the moving body. The review includes (1) vertical and oblique water entry of a body at constant or a prescribed varying speed, as well as free fall motion, (2) liquid droplets or column impact as well as wave impact on a body, (3) similarity solution of an expanding body. It covers two dimensional (2D), axisymmetric and three dimensional (3D) cases. Key techniques used in the numerical simulation are outlined, including mesh generation on the multivalued free surface, the stretched coordinate system for expanding domain, the auxiliary function method for decoupling the mutual dependence of the pressure and the body motion, and treatment for the jet or the thin liquid film developed during impact. 展开更多
关键词 fluid/structure impact boundary element method 3D surface mesh generation water entry wave impact similarity solution fully nonlinear simulation
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Research on Nonlinear Dynamic Simulation and Fatigue Reliability Life Prediction for Synthesis Transmission System of Self-Propelled Gun 被引量:1
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作者 YU Guibo CAO Lijun +1 位作者 WANG Shuhai MA Qiao 《Journal of Shanghai Jiaotong university(Science)》 EI 2018年第6期776-783,共8页
Aiming to the puzzle that the inner load of nonlinear synthesis transmission system is difficult to obtain,a new kind of virtual prototype establishment and simulation method is put forward. The influence on nonlinear... Aiming to the puzzle that the inner load of nonlinear synthesis transmission system is difficult to obtain,a new kind of virtual prototype establishment and simulation method is put forward. The influence on nonlinear vibration with flexible rotor, bearing backlash is analyzed based on a virtual prototype. To validate the virtual prototype of nonlinear gear transmission system, the corresponding test platform is established. The consistency between simulation results and test results proves that the simulation results of the virtual prototype can be used to calculate the fatigue reliability life of key components. A new kind of fatigue reliability life prediction method of gear system considering multi-random parameter distribution is put forward based on the fativipatistic theory. Considering the periodicity of gear meshing, linear interpolation method is adopted to obtain the stress-time course of random load spectrum based on the gear's complicated torque provided by virtual prototype.The gear's P-Sa-Sm-N curved cluster can be simulated based on material's P-S-N curve. The simulation process considers the parameter distributions of stress concentration coefficients, dimension coefficients and surface quality treatment coefficients, and settles the puzzle that traditional test methods cannot acquire the gear's fatigue life of all reliability levels. This method can provide the distribution function and the interval of fatigue reliability life of gear's danger region, and has a guide meaning for the gear maintenance periods determination and reliability evaluation. 展开更多
关键词 synthesis transmission system virtual prototype nonlinear dynamic simulation fatigue reliability life prediction
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TWO-DIMENSIONAL NONLINEAR DYNAMIC SYSTEM MODEL OF INTERSPECIFIC INTERACTION AND NUMERICAL SIMULATION RESEARCH ON IT
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作者 李自珍 徐彩琳 王万雄 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2003年第7期836-844,共9页
The mechanism and the course of two_dimensional nonlinear dynamic system of interspecific interaction were dealt with systematically. By extending the Lotka_Volterra model from the viewpoint of biomechanics, it develo... The mechanism and the course of two_dimensional nonlinear dynamic system of interspecific interaction were dealt with systematically. By extending the Lotka_Volterra model from the viewpoint of biomechanics, it developed new models of two_dimensional nonlinear autonomous and nonautonomous dynamic systems, with its equilibrium point's stability and the existence and stability of its periodical solutions analyzed, and did numerical simulation experiments on its dynamics course. The results show that efficiency of interaction between two populations, time_varying effort, and change direction of action coefficient and reaction coefficient have important influences on the stability of dynamic system, that too large or too small interspecific interaction efficiency and contrary change direction of action coefficient and reaction coefficient may result in the nonstability of the system, and thus it is difficult for two populations to coexist, and that time_varying active force contributes to system stability. 展开更多
关键词 two_dimensional nonlinear dynamic system interspecific interaction EFFORT numerical simulation
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Freak wave simulation based on nonlinear model and the research on the time-frequency energy spectrum of simulation results
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作者 崔成 张宁川 李靖波 《Marine Science Bulletin》 CAS 2011年第1期25-39,共15页
VOF (volume of fluid) method has been used to make the numerical simulation of freak wave come true. The comparisons between the numerical results and linear theoretical results corresponding to Eq.(5) have been c... VOF (volume of fluid) method has been used to make the numerical simulation of freak wave come true. The comparisons between the numerical results and linear theoretical results corresponding to Eq.(5) have been carried out to show that the numerical results have a better exhibition of nonlinear characteristics. Wavelet analysis method has been adopted to investigate the time-frequency energy spectrum of simulation freak waves and the results reveal strong nonlinear interaction enables energy to be transferred to high harmonics during the progress of its formation. Varying water depth can enhance the nonlinear interaction, making much more energy be transferred to high harmonics and freak waves with higher asymmetry be generated. 展开更多
关键词 freak wave numerical simulation wavelet analysis varying water depthtime-frequency energy spectrum
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Numerical simulation for the two-dimensional nonlinear shallow water waves
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作者 Tao Jianhua and Zhang Yan Department of Mechanics, Tianjin University, Tianjin, China 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1990年第3期449-457,共9页
This study deals with the general numerical model to simulate the two-dimensional tidal flow, flooding wave (long wave) and shallow water waves (short wave). The foundational model is based on nonlinear Boussinesq equ... This study deals with the general numerical model to simulate the two-dimensional tidal flow, flooding wave (long wave) and shallow water waves (short wave). The foundational model is based on nonlinear Boussinesq equations. Numerical method for modelling the short waves is investigated in detail. The forces, such as Coriolis forces, wind stress, atmosphere and bottom friction, are considered. A two-dimensional implicit difference scheme of Boussinesq equations is proposed. The low-reflection outflow open boundary is suggested. By means of this model,both velocity fields of circulation current in a channel with step expansion and the wave diffraction behind a semi-infinite breakwater are computed, and the results are satisfactory. 展开更多
关键词 Numerical simulation for the two-dimensional nonlinear shallow water waves LENGTH THAN
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Numerical Simulation of Two-dimensional Nonlinear Sloshing Problems
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作者 梁志勇 刘东顺 严承华 《Journal of Donghua University(English Edition)》 EI CAS 2005年第4期41-46,共6页
Numerical simulation of a two-dimensional nonlinear sloshing problem is preceded by the finite element method. Two theories are used. One is fully nonlinear theory; the other is time domain second order theory. A liqu... Numerical simulation of a two-dimensional nonlinear sloshing problem is preceded by the finite element method. Two theories are used. One is fully nonlinear theory; the other is time domain second order theory. A liquid sloshing in a rectangular container subjected to a horizontal excitation is simulated using these two theories. Numerical results are obtained and comparisons are made. It is found that a good agreement is obtained for the case of small amplitude oscillation. For the situation of large amplitude excitation, although the differences between using the two theories are obvious the second order solution can still exhibit typical nonlinear features of nonlinear wave. 展开更多
关键词 numerical simulation sloshing problem finite element nonlinear theory time domain second order theory.
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Realization of nonlinear PID with feed-forward controller for 3-DOF flight simulator and hardware-in-the-loop simulation 被引量:3
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作者 Duan Haibin Wang Daobo Yu Xiufen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2008年第2期342-345,共4页
As friction, intrinsic steady-state nonlinearity poses a challenging dilemma to the control system of 3-DOF (three degree of freedom) flight simulator, a novel hybrid control strategy of nonlinear PID (proportional... As friction, intrinsic steady-state nonlinearity poses a challenging dilemma to the control system of 3-DOF (three degree of freedom) flight simulator, a novel hybrid control strategy of nonlinear PID (proportionalintegral-derivative) with additional FFC (feed-forward controller) is proposed, and the hardware-in-the-loop simulation results are also given. Based on the description of 3-DOF flight simulator, a novel nonlinear PID theory is well introduced. Then a nonlinear PID controller with additional FFC is designed. Subsequently, the loop structure of 3-DOF flight simulator is also designed. Finally, a series of hardware-in-the-loop simulation experiments are undertaken to verify the feasibility and effectiveness of the proposed nonlinear PID controller with additional FFC for 3-DOF flight simulator. 展开更多
关键词 flight simulator nonlinear PID FFC hardware-in-the-loop.
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Accuracy of three-dimensional seismic ground response analysis in time domain using nonlinear numerical simulations 被引量:7
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作者 Liang Fayun Chen Haibing Huang Maosong 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2017年第3期487-498,共12页
To provide appropriate uses of nonlinear ground response analysis for engineering practice, a three-dimensional soil column with a distributed mass system and a time domain numerical analysis were implemented on the O... To provide appropriate uses of nonlinear ground response analysis for engineering practice, a three-dimensional soil column with a distributed mass system and a time domain numerical analysis were implemented on the Open Sees simulation platform. The standard mesh of a three-dimensional soil column was suggested to be satisfied with the specified maximum frequency. The layered soil column was divided into multiple sub-soils with a different viscous damping matrix according to the shear velocities as the soil properties were significantly different. It was necessary to use a combination of other one-dimensional or three-dimensional nonlinear seismic ground analysis programs to confirm the applicability of nonlinear seismic ground motion response analysis procedures in soft soil or for strong earthquakes. The accuracy of the three-dimensional soil column finite element method was verified by dynamic centrifuge model testing under different peak accelerations of the earthquake. As a result, nonlinear seismic ground motion response analysis procedures were improved in this study. The accuracy and efficiency of the three-dimensional seismic ground response analysis can be adapted to the requirements of engineering practice. 展开更多
关键词 three dimensional soil column seismic ground response centrifugal model test nonlinear analysis accuracyverification
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NONLINEAR INVERSE SIMULATION FOR THE MANEUVERING FLIGHT OF COAXIAL ROTOR HELICOPTERS 被引量:3
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作者 Cao Yihua (Institute of Aircraft Design, Beijing University of Aeronautics and Astronautics, Beijing, 100083, China) G. Reichert (Technical University of Braunschweig, 38106 Braunschweig, Germany) 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 1997年第4期2-9,共8页
The maneuvering flight governing equations for coaxial rotor helicopters are established. By introducing induced velocity interference factor analysis, the coaxial rotor aerodynamic interference can be taken into acc... The maneuvering flight governing equations for coaxial rotor helicopters are established. By introducing induced velocity interference factor analysis, the coaxial rotor aerodynamic interference can be taken into account. With the combination of coaxial rotor helicopter control features and nonlinear inverse solution technique, the governing equations for maneuvering flight can be solved so as to determine helicopter control input, control force and moment, and helicopter body attitudes which are needed for performing the defined maneuver. Finally, as an example of this methods engineering application, the calculated results with level turn, lateral jink maneuvers are presented and simply analyzed. 展开更多
关键词 helicopters rotor aerodynamics MANEUVERS FLIGHT inverse simulation
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Fully Nonlinear Simulations of Wave Resonance by An Array of Cylinders in Vertical Motions 被引量:3
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作者 黄豪彩 王赤忠 冷建兴 《China Ocean Engineering》 SCIE EI CSCD 2013年第1期87-98,共12页
The finite element method (FEM) is employed to analyze the resonant oscillations of the liquid confined within multiple or an array of floating bodies with fully nonlinear boundary conditions on the free surface and... The finite element method (FEM) is employed to analyze the resonant oscillations of the liquid confined within multiple or an array of floating bodies with fully nonlinear boundary conditions on the free surface and the body surface in two dimensions. The velocity potentials at each time step are obtained through the FEM with 8-node quadratic shape functions. The finite element linear system is solved by the conjugate gradient (CG) method with a symmetric successive overelaxlation (SSOR) preconditioner. The waves at the open boundary are absorbed by the combination of the damping zone method and the Sommerfeld-Orlanski equation. Numerical examples are given by an array of floating wedge- shaped cylinders and rectangular cylinders. Results are provided for heave motions including wave elevations, profiles and hydrodynamic forces. Comparisons are made in several cases with the results obtained from the second order solution in the time domain. It is found that the wave amplitude in the middle region of the array is larger than those in other places, and the hydrodynamic force on a cylinder increases with the cylinder closing to the middle of the array. 展开更多
关键词 RESONANCE an array of cylinders fully nonlinear wave high order finite element method
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Nonlinear compensation research and simulation of bandlimited QPSK digital satellite channels 被引量:1
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作者 何茂平 顾学迈 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2000年第1期46-49,共4页
Presents a phase compensation against the signal distortion mainly caused by TWTA to solve the problem of compensation and modelling of a nonlinear QPSK satellite channel assumed to be bandlimited and exhibit both amp... Presents a phase compensation against the signal distortion mainly caused by TWTA to solve the problem of compensation and modelling of a nonlinear QPSK satellite channel assumed to be bandlimited and exhibit both amplitude and phase nonlinearities, and to lower the bit error probability of nonlinear channel, and concludes with simulation results that the compensation against phase distortion of TWTA can significantly improve the nonlinear performance of the channel. 展开更多
关键词 QPSK SATELLITE CHANNEL nonlinearITY PHASE COMPENSATION
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Nonlinear Simulation of Focused Wave Group Action on a Truncated Surface-Piercing Structure 被引量:1
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作者 Dezhi Ning Xiang Li Chongwei Zhang 《Journal of Marine Science and Application》 CSCD 2018年第3期362-370,共9页
In this study, we numerically investigated the nonlinear focused wave group action on a truncated surface-piercing structure, and developed a two-dimensional fully nonlinear numerical tank using the higher-order bound... In this study, we numerically investigated the nonlinear focused wave group action on a truncated surface-piercing structure, and developed a two-dimensional fully nonlinear numerical tank using the higher-order boundary element method. We determined the amplitude of the wave components of the focused wave group based on the JONSWAP wave spectrum. We discuss the effects of the presence of a surface-piercing structure on the characteristics of the focused wave group and find that the location of the structure does not evidently change the focal location or focal time of the focused wave group. The largest amplitudes of the run-up and horizontal force on the structure occur when the front surface of the structure is at the focal location. The critical draught and breadth of the structure occur when the wave run-up reaches its maximum along the structure. 展开更多
关键词 FOCUSED wave Fully nonlinear Numerical wavetank FLOATING BODY BOUNDARY element method
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Strain-threshold- and frequency-dependent seismic simulation of nonlinear soils 被引量:1
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作者 Duguo Wang Chenggang Zhao 《Earthquake Science》 2014年第6期615-626,共12页
A one-dimensional equivalent linear method (EQL) is widely used in estimating seismic ground response. For this method, the shear modulus and damping ratio of inelastic soil are supposed to be frequency independent.... A one-dimensional equivalent linear method (EQL) is widely used in estimating seismic ground response. For this method, the shear modulus and damping ratio of inelastic soil are supposed to be frequency independent. However, historical earthquake records and laboratory test results indicate that nonlinear soil behavior is frequency- dependent. Several frequency-dependent equivalent linear methods (FDEQL) related to the Fourier amplitude of shear strain time history have been developed to take into account the frequency-dependent soil behavior. Furthermore, the shear strain threshold plays an important role in soil behavior. For shear strains below the elastic shear strain threshold, soil behaves essentially as a linear elastic mate- rial. To consider the effect of elastic-shear-strain-threshold- and frequency-dependent soil behavior on wave propaga- tion, the shear-strain-threshold- and frequency-dependent equivalent linear method (TFDEQL) is proposed. A series of analyses is implemented for EQL, FDEQL, and TFDEQL methods. Results show that elastic-shear-strain-threshold- and frequency-dependent soil behavior plays a great influence on the computed site response, especially for the high- frequency band. Also, the effect of elastic-strain-threshold- and frequency-dependent soil behavior on the site response is analyzed from relatively weak to strong input motion, and results show that the effect is more pronounced as input motion goes from weak to strong. 展开更多
关键词 nonlinear soil behavior Strain threshold Frequency-dependent Site response
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Nonlinear Mathematical Simulation and Analysis of Dha Regulon for Glycerol Metabolism in Klebsiella pneumoniae 被引量:5
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作者 孙亚琴 叶剑雄 +4 位作者 牟晓佳 滕虎 冯恩民 曾安平 修志龙 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2012年第5期958-970,共13页
Glycerol may be converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under anaerobic conditions and glycerol dismutation involves two parallel pathways controlled by the dha regulon. In this study, a fourtee... Glycerol may be converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under anaerobic conditions and glycerol dismutation involves two parallel pathways controlled by the dha regulon. In this study, a fourteen-dimensional nonlinear dynamic system is presented to describe the continuous culture and multiplicity analysis, in which two regulated negative-feedback mechanisms of repression and enzyme inhibition are investigated. The model describing the expression of gene-mRNA-enzyme-product was established according to the repression of the dha regulon by 3-hydroxypropionaldehy (3-HPA). Comparisons between simulated and experimental results indicate that the model can be used to describe the production of 1,3-PD under continuous fermentation. The new model is translated into the corresponding S-system version. The robustness of this model is discussed by using the S-system model and the sensitivity analysis shows that the model is sufficiently robust. The influences of initial glycerol concentration and dilution rate on the biosynthesis of 1,3-PD and the stability of the dha regulon model are investigated. The intracellular concentrations of glycerol, 1,3-PD, 3-HPA, repressor mRNA, repressor, mRNA and protein levels of glycerol dehydratase (GDHt) and 1,3-PD oxydoreductase (PDOR) can be predicted for continuous cultivation. The results of simulation and analysis indicate that 3-HPA accumulation will repress the expression of the dha regulon at the transcriptional level. This model gives new insights into the regulation of glycerol metabolism in K. pneumoniae and explain some of the experimental observations. 展开更多
关键词 dha regulon ROBUSTNESS nonlinear dynamics genetic regulation 3-hydroxypropionaldehy
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