Determining the crossing number of a given graph is NP-complete. The cycle of length m is denoted by Cm = v1v2…vmv1. G^((1))_(m) (m ≥ 5) is the graph obtained from Cm by adding two edges v1v3 and vlvl+2 (3 ≤ l ≤ m...Determining the crossing number of a given graph is NP-complete. The cycle of length m is denoted by Cm = v1v2…vmv1. G^((1))_(m) (m ≥ 5) is the graph obtained from Cm by adding two edges v1v3 and vlvl+2 (3 ≤ l ≤ m−2), G^((2))m (m ≥ 4) is the graph obtained from Cm by adding two edges v1v3 and v2v4. The famous Zarankiewicz’s conjecture on the crossing number of the complete bipartite graph Km,n states that cr(Km,n)=Z(m,n)=[m/2][m-1/2][n/2[n-1/2].Based on Zarankiewicz’s conjecture, a natural problem is to study the change in the crossingnumber of the graphs obtained from the complete bipartite graph by adding certain edge sets.If Zarankiewicz’s conjecture is true, this paper proves that cr(G^((1))_(m)+Kn)=Z(m,n)+2[n/2] and cr(G^((2))_(m)+Kn)=Z(m,n)+n.展开更多
Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and hom...Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and homologous recombination(HR)being the two most prominent.Although two major pathways have been extensively studied in Arabidopsis,rice and other mammals,the exact functions and differences between the two DSB repair pathways in maize still remain less well understood.Here,we characterized mre11a and rad50,mutants of HR pathway patterns,which showed drastic degradation of the typically persistent embryo and endosperm during kernel development.Loss of MRE11 or RAD50 function led to chromosomal fragments and chromosomal bridges in anaphase.While we also reported that the NHEJ pathway patterns,KU70 and KU80 are associated with developmental growth and genome stability.ku70 and ku80 both displayed an obvious dwarf phenotype.Cytological analysis of the mutants revealed extensive chromosome fragmentation in metaphase and subsequent stages.Loss of KU70/80 function upregulated the expression of genes involved in cell cycle progression and nuclear division.These results provide insights into how NHEJ and HR are mechanistically executed during different plant developmental periods and highlight a competitive and complementary relationship between the NHEJ and HR pathways for DNA double-strand break repair in maize.展开更多
The development and application of large Die⁃Casting Al Alloy(DCAA)parts and Thermo⁃Formed Steel Sheets(TFSS)in Body⁃in⁃White(BIW)have created higher demands for the joining technology of high⁃strength steel/Al dissim...The development and application of large Die⁃Casting Al Alloy(DCAA)parts and Thermo⁃Formed Steel Sheets(TFSS)in Body⁃in⁃White(BIW)have created higher demands for the joining technology of high⁃strength steel/Al dissimilar materials.As an emerging technology,Flush Self⁃Piercing Riveting(FSPR)is still in the experimental phase and undergoing small batch equipment verification.This paper focuses on the joining methods for DCAA and TFSS in BIW,investigating the joining mechanisms,technical features,and forming principles of FSPR for steel/Al dissimilar materials with two⁃layer or three⁃layer plate combinations.Considering the TL4225/C611/CR5 sheet combination as a subject,the forming mechanism of high⁃quality joints was studied,and a physical and mathematical model was established to depict the relationship between the filling amount of the arc⁃gap and die dimensions,as well as the extrusion amount.This model effectively illustrates the relationship between the filling amount of the flowing metal in the arc⁃gap and critical parameters,such as die dimensions and feeding amounts.By simplifying the process of selecting joining parameters,it significantly reduces both the time and experimental workload associated with parameter selection.This provides a technical foundation for the application of DAAA and TFSS parts in BIW,enabling the rapid choice of appropriate joining parameters to meet the requirements for obtaining high⁃quality joints.The model can be effectively utilized to investigate the relationships between key parameters,including arc⁃gap radius,plate thickness,rivet arc radius,nail head radius,groove width,and feeding amount,while keeping other parameters constant.This approach provides a theoretical foundation for the design of Friction Stir Processing(FSP)joints and aids in the selection of optimal parameters.展开更多
Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/...Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/dissimilar RFSSW joint exhibits remarkable and endurable characteristics,including high shear strength,long fatigue life,and strong corrosion resistance.In the meanwhile,as the key-hole free joint has different microstructures compared with conventional friction stir spot welding,thus the RFSSW joint shall possess different shear and fatigue fracture mechanisms,which needs further investigation.To explore the underlying failure mechanism,the similar/dissimilar metallic material joining parameters and pre-treatment,mechanical properties,as well as fracture mechanisms under this novel technology will be discussed.In details,the welding tool design,welding parameters setting,and the influence of processing on the lap shear and fatigue properties,as well as the corrosion resistance will be mainly discussed.Moreover,the roadmap of RFFSW is also discussed.展开更多
Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeu...Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA repair.Among the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential role.In this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in vivo.Conversely,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP treatment.Mechanistically,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of CDK1.Meanwhile,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy drugs.Our transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant cells.In summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.展开更多
Brazing,an important welding and joining technology,can achieve precision joining of materials in advanced manufacturing.And the first principle calculation is a new material simulation method in high-throughput compu...Brazing,an important welding and joining technology,can achieve precision joining of materials in advanced manufacturing.And the first principle calculation is a new material simulation method in high-throughput computing.It can calculate the interfacial structure,band structure,electronic structure,and other properties between dissimilar materials,predicting various properties.It plays an important role in assisting practical research and guiding experimental designs by predicting material properties.It can largely improve the quality of welded components and joining efficiency.The relevant theoretical foundation is reviewed,including the first principle and density functional theory.Exchange-correlation functional and pseudopotential plane wave approach was also introduced.Then,the latest research progress of the first principle in brazing was also summarized.The application of first principle calculation mainly includes formation energy,adsorption energy,surface energy,adhesion work,interfacial energy,interfacial contact angle,charge density differences,density of states,and mulliken population.The energy,mechanical,and electronic properties were discussed.Finally,the limitations and shortcomings of the research in the first principle calculation of brazed interface were pointed out.Future developmental directions were presented to provide reference and theoretical basis for realizing high-throughput calculations of brazed joint interfaces.展开更多
Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRP...Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRPs)through laser-assisted metal and plastic direct joining(LAMP).This study uses the LAMP technique to produce AZ31-CFRP joints.The joining process involves as-received AZ31,HFpretreated AZ31,and thermally oxidized HF-pretreated AZ31 alloy sheets.Furthermore,the bonding strength of joints prepared with thermally oxidized AZ31 alloy sheets is examined to ascertain the combined effect of HF treatment and thermal oxidation on bonding strength.The microstructures,surface chemical interactions,and mechanical performances of joints are investigated under tensile shear loading.Various factors,such as bubble formation,CFRP resin decomposition,and mechanical interlocking considerably affect joint strength.Additionally,surface chemical interactions between the active species on metal parts and the polar amide along with carbonyl groups of polymer play a significant role in improving joint strength.Joints prepared with surface-pretreated AZ31 alloy sheets show significant improvements in bonding strength.展开更多
COP29-the 29th session of the Conference of the Parties to the United Nations Framework Convention on Climate Change-has accentuated the urgency of jointly addressing climate change,our common global threat,by pushing...COP29-the 29th session of the Conference of the Parties to the United Nations Framework Convention on Climate Change-has accentuated the urgency of jointly addressing climate change,our common global threat,by pushing for a fair and ambitious new climate financing target.It has also brought into the global spotlight China’s progress in green transition and carbon emission reduction and its commitment to international cooperation.展开更多
Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward high...Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward higher requirements for the application of joining technology of high-strength steel/Al dissimilar materials. Taking the new die-casting Al alloy body as an example, this paper systematically studies the progress of the latest joining methods of steel/Al dissimilar material with combination of two-layer plate and three-layer plate. By analyzing the joining technologies such as FSPR, RES, FDS and SPR, the technology and process characteristics of steel/Al dissimilar material joining are studied, and the joining technical feasibility and realization means of different material combination of the body are analyzed. The conditions of material combination, material thickness, material strength, flange height, preformed holes and joint spacing for achieving high-quality joining are given. The FSPR joining technology is developed and tested in order to meet with the joining of parts with DCAA and TFSS, especially for the joining of three-layer plates with them. It finds the method and technical basis for the realization of high quality joining of dissimilar materials, provides the early conditions for the application of large DCAA and TFSS parts in body-in-white, and meets the design requirements of new energy body. .展开更多
基金Supported by Changsha Natural Science Foundation(No.kq2208001)the Key Project Funded by Hunan Provincial Department of Education(No.21A0590)。
文摘Determining the crossing number of a given graph is NP-complete. The cycle of length m is denoted by Cm = v1v2…vmv1. G^((1))_(m) (m ≥ 5) is the graph obtained from Cm by adding two edges v1v3 and vlvl+2 (3 ≤ l ≤ m−2), G^((2))m (m ≥ 4) is the graph obtained from Cm by adding two edges v1v3 and v2v4. The famous Zarankiewicz’s conjecture on the crossing number of the complete bipartite graph Km,n states that cr(Km,n)=Z(m,n)=[m/2][m-1/2][n/2[n-1/2].Based on Zarankiewicz’s conjecture, a natural problem is to study the change in the crossingnumber of the graphs obtained from the complete bipartite graph by adding certain edge sets.If Zarankiewicz’s conjecture is true, this paper proves that cr(G^((1))_(m)+Kn)=Z(m,n)+2[n/2] and cr(G^((2))_(m)+Kn)=Z(m,n)+n.
基金supported by the National Natural Science Foundation of China(32372116)to Yan He.
文摘Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and homologous recombination(HR)being the two most prominent.Although two major pathways have been extensively studied in Arabidopsis,rice and other mammals,the exact functions and differences between the two DSB repair pathways in maize still remain less well understood.Here,we characterized mre11a and rad50,mutants of HR pathway patterns,which showed drastic degradation of the typically persistent embryo and endosperm during kernel development.Loss of MRE11 or RAD50 function led to chromosomal fragments and chromosomal bridges in anaphase.While we also reported that the NHEJ pathway patterns,KU70 and KU80 are associated with developmental growth and genome stability.ku70 and ku80 both displayed an obvious dwarf phenotype.Cytological analysis of the mutants revealed extensive chromosome fragmentation in metaphase and subsequent stages.Loss of KU70/80 function upregulated the expression of genes involved in cell cycle progression and nuclear division.These results provide insights into how NHEJ and HR are mechanistically executed during different plant developmental periods and highlight a competitive and complementary relationship between the NHEJ and HR pathways for DNA double-strand break repair in maize.
文摘The development and application of large Die⁃Casting Al Alloy(DCAA)parts and Thermo⁃Formed Steel Sheets(TFSS)in Body⁃in⁃White(BIW)have created higher demands for the joining technology of high⁃strength steel/Al dissimilar materials.As an emerging technology,Flush Self⁃Piercing Riveting(FSPR)is still in the experimental phase and undergoing small batch equipment verification.This paper focuses on the joining methods for DCAA and TFSS in BIW,investigating the joining mechanisms,technical features,and forming principles of FSPR for steel/Al dissimilar materials with two⁃layer or three⁃layer plate combinations.Considering the TL4225/C611/CR5 sheet combination as a subject,the forming mechanism of high⁃quality joints was studied,and a physical and mathematical model was established to depict the relationship between the filling amount of the arc⁃gap and die dimensions,as well as the extrusion amount.This model effectively illustrates the relationship between the filling amount of the flowing metal in the arc⁃gap and critical parameters,such as die dimensions and feeding amounts.By simplifying the process of selecting joining parameters,it significantly reduces both the time and experimental workload associated with parameter selection.This provides a technical foundation for the application of DAAA and TFSS parts in BIW,enabling the rapid choice of appropriate joining parameters to meet the requirements for obtaining high⁃quality joints.The model can be effectively utilized to investigate the relationships between key parameters,including arc⁃gap radius,plate thickness,rivet arc radius,nail head radius,groove width,and feeding amount,while keeping other parameters constant.This approach provides a theoretical foundation for the design of Friction Stir Processing(FSP)joints and aids in the selection of optimal parameters.
基金This work was supported by International Science and Technology Cooperation Project of Guangdong Province(Grant No.2022A0505050054)Innovation and Technology Fund(ITF)(Grant No.ITP/021/19AP)National Natural Science Foundation of China(Grant No.51905112).
文摘Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/dissimilar RFSSW joint exhibits remarkable and endurable characteristics,including high shear strength,long fatigue life,and strong corrosion resistance.In the meanwhile,as the key-hole free joint has different microstructures compared with conventional friction stir spot welding,thus the RFSSW joint shall possess different shear and fatigue fracture mechanisms,which needs further investigation.To explore the underlying failure mechanism,the similar/dissimilar metallic material joining parameters and pre-treatment,mechanical properties,as well as fracture mechanisms under this novel technology will be discussed.In details,the welding tool design,welding parameters setting,and the influence of processing on the lap shear and fatigue properties,as well as the corrosion resistance will be mainly discussed.Moreover,the roadmap of RFFSW is also discussed.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.:82003807,82173394)the Shaanxi Province Science Foundation,China(Grant No.:2023-GHZD-19)+1 种基金the Medical Foundation-Clinical Integration Program of Xi'an Jiaotong University,China(Grant No.:YXJLRH2022043)the Xi'an Jiaotong University Free Exploration and Innovation-Teacher Project Foundation,China(Grant No.:xzy012023104).
文摘Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA repair.Among the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential role.In this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in vivo.Conversely,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP treatment.Mechanistically,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of CDK1.Meanwhile,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy drugs.Our transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant cells.In summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.
基金financially supported by National Natural Science Foundation of China(52475347,52071165)National Foreign Experts Program of Ministry of Science and Technology(G2023026003L)+2 种基金China Postdoctoral Fund(2023M740475)Henan Provincial Science and Technology Joint Fund(Industry)(225101610002)Program for Science&Technology Innovation Talents in Universities of Henan Province,China(22HASTIT026),International Science and Technology Cooperation Project of Henan Province(242102521057),China,the Program for the Top Young Talents of Henan Province,China and Frontier Exploration Project of Longmen Laboratory(LMQYTSKT016),China.
文摘Brazing,an important welding and joining technology,can achieve precision joining of materials in advanced manufacturing.And the first principle calculation is a new material simulation method in high-throughput computing.It can calculate the interfacial structure,band structure,electronic structure,and other properties between dissimilar materials,predicting various properties.It plays an important role in assisting practical research and guiding experimental designs by predicting material properties.It can largely improve the quality of welded components and joining efficiency.The relevant theoretical foundation is reviewed,including the first principle and density functional theory.Exchange-correlation functional and pseudopotential plane wave approach was also introduced.Then,the latest research progress of the first principle in brazing was also summarized.The application of first principle calculation mainly includes formation energy,adsorption energy,surface energy,adhesion work,interfacial energy,interfacial contact angle,charge density differences,density of states,and mulliken population.The energy,mechanical,and electronic properties were discussed.Finally,the limitations and shortcomings of the research in the first principle calculation of brazed interface were pointed out.Future developmental directions were presented to provide reference and theoretical basis for realizing high-throughput calculations of brazed joint interfaces.
基金supported by the Nano&Material Technology Development Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(RS-2023-00234757).
文摘Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRPs)through laser-assisted metal and plastic direct joining(LAMP).This study uses the LAMP technique to produce AZ31-CFRP joints.The joining process involves as-received AZ31,HFpretreated AZ31,and thermally oxidized HF-pretreated AZ31 alloy sheets.Furthermore,the bonding strength of joints prepared with thermally oxidized AZ31 alloy sheets is examined to ascertain the combined effect of HF treatment and thermal oxidation on bonding strength.The microstructures,surface chemical interactions,and mechanical performances of joints are investigated under tensile shear loading.Various factors,such as bubble formation,CFRP resin decomposition,and mechanical interlocking considerably affect joint strength.Additionally,surface chemical interactions between the active species on metal parts and the polar amide along with carbonyl groups of polymer play a significant role in improving joint strength.Joints prepared with surface-pretreated AZ31 alloy sheets show significant improvements in bonding strength.
文摘COP29-the 29th session of the Conference of the Parties to the United Nations Framework Convention on Climate Change-has accentuated the urgency of jointly addressing climate change,our common global threat,by pushing for a fair and ambitious new climate financing target.It has also brought into the global spotlight China’s progress in green transition and carbon emission reduction and its commitment to international cooperation.
文摘Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward higher requirements for the application of joining technology of high-strength steel/Al dissimilar materials. Taking the new die-casting Al alloy body as an example, this paper systematically studies the progress of the latest joining methods of steel/Al dissimilar material with combination of two-layer plate and three-layer plate. By analyzing the joining technologies such as FSPR, RES, FDS and SPR, the technology and process characteristics of steel/Al dissimilar material joining are studied, and the joining technical feasibility and realization means of different material combination of the body are analyzed. The conditions of material combination, material thickness, material strength, flange height, preformed holes and joint spacing for achieving high-quality joining are given. The FSPR joining technology is developed and tested in order to meet with the joining of parts with DCAA and TFSS, especially for the joining of three-layer plates with them. It finds the method and technical basis for the realization of high quality joining of dissimilar materials, provides the early conditions for the application of large DCAA and TFSS parts in body-in-white, and meets the design requirements of new energy body. .
