In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementi...In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementing robust security measures in modern web applications and the proof of the Methodology applied to Vue.js,Spring Boot,and MySQL architecture.The proposed approach addresses critical security challenges through a multi-layered framework that encompasses essential security dimensions including multi-factor authentication,fine-grained authorization controls,sophisticated session management,data confidentiality and integrity protection,secure logging mechanisms,comprehensive error handling,high availability strategies,advanced input validation,and security headers implementation.Significant contributions are made to the field of web application security.First,a detailed catalogue of security requirements specifically tailored to protect web applications against contemporary threats,backed by rigorous analysis and industry best practices.Second,the methodology is validated through a carefully designed proof-of-concept implementation in a controlled environment,demonstrating the practical effectiveness of the security measures.The validation process employs cutting-edge static and dynamic analysis tools for comprehensive dependency validation and vulnerability detection,ensuring robust security coverage.The validation results confirm the prevention and avoidance of security vulnerabilities of the methodology.A key innovation of this work is the seamless integration of DevSecOps practices throughout the secure Software Development Life Cycle(SSDLC),creating a security-first mindset from initial design to deployment.By combining proactive secure coding practices with defensive security approaches,a framework is established that not only strengthens application security but also fosters a culture of security awareness within development teams.This hybrid approach ensures that security considerations are woven into every aspect of the development process,rather than being treated as an afterthought.展开更多
Background Early embryo development plays a pivotal role in determining pregnancy outcomes,postnatal development,and lifelong health.Therefore,the strategic selection of functional nutrients to enhance embryo developm...Background Early embryo development plays a pivotal role in determining pregnancy outcomes,postnatal development,and lifelong health.Therefore,the strategic selection of functional nutrients to enhance embryo development is of paramount importance.In this study,we established a stable porcine trophectoderm cell line expressing dual fluorescent reporter genes driven by the CDX2 and TEAD4 gene promoter segments using lentiviral transfection.Results Three amino acid metabolites—kynurenic acid,taurine,and tryptamine—met the minimum z-score criteria of 2.0 for both luciferase and Renilla luciferase activities and were initially identified as potential metabolites for embryo development,with their beneficial effects validated by qPCR.Given that the identified metabolites are closely related to methionine,arginine,and tryptophan,we selected these three amino acids,using lysine as a standard,and employed response surface methodology combined with our high-throughput screening cell model to efficiently screen and optimize amino acid combination conducive to early embryo development.The optimized candidate amino acid system included lysine(1.87 mmol/L),methionine(0.82 mmol/L),tryptophan(0.23 mmol/L),and arginine(3 mmol/L),with the ratio of 1:0.43:0.12:1.60.In vitro experiments confirmed that this amino acid system enhances the expression of key genes involved in early embryonic development and improves in vitro embryo adhesion.Transcriptomic analysis of blastocysts suggested that candidate amino acid system enhances early embryo development by regulating early embryonic cell cycle and differentiation,as well as improving nutrient absorption.Furthermore,based on response surface methodology,400 sows were used to verify this amino acid system,substituting arginine with the more cost-effective N-carbamoyl glutamate(NCG),a precursor of arginine.The optimal dietary amino acid requirement was predicted to be 0.71%lysine,0.32%methionine,0.22%tryptophan,and 0.10%NCG for sows during early gestation.The optimized amino acid system ratio of the feed,derived from the peripheral release of essential amino acids,was found to be 1:0.45:0.13,which is largely consistent with the results obtained from the cell model optimization.Subsequently,we furtherly verified that this optimal dietary amino acid system significantly increased total litter size,live litter size and litter weight in sows.Conclusions In summary,we successfully established a dual-fluorescent high-throughput screening cell model for the efficient identification of potential nutrients that would promote embryo development and implantation.This innovative approach overcomes the limitations of traditional amino acid nutrition studies in sows,providing a more effective model for enhancing reproductive outcomes.展开更多
Product Master Model(PMM)Technology is a philosophy and methodology for organizing,managing and controlling product development processes,sharing the information and knowledge of the product and its processes.In this ...Product Master Model(PMM)Technology is a philosophy and methodology for organizing,managing and controlling product development processes,sharing the information and knowledge of the product and its processes.In this paper,the concept of PMM is proposes,the basic theory of PMM technologies is studied,the blackboard architecture of PMM is proposed,the product development process framework is explored,and the prototype system of PMM is developed.展开更多
This white paper explores three popular development methodologies for network softwarization: DevOps, NetOps, and Verification. The paper compares and contrasts the strengths and weaknesses of each approach and provid...This white paper explores three popular development methodologies for network softwarization: DevOps, NetOps, and Verification. The paper compares and contrasts the strengths and weaknesses of each approach and provides recommendations for organizations looking to adopt network softwarization.展开更多
Objectives:Electronic health records(EHRs)offer valuable real-world data(RWD)for Chinese medicine research.However,significant methodological challenges remain in developing integrative Chinese-Western medicine(ICWM)d...Objectives:Electronic health records(EHRs)offer valuable real-world data(RWD)for Chinese medicine research.However,significant methodological challenges remain in developing integrative Chinese-Western medicine(ICWM)databases.This study aims to establish a best-practice methodological framework,referred to as BRIDGE,to guide the construction of ICWM databases using EHRs.Methods:We developed the methodological framework through a comprehensive process,including systematic literature review,synthesis of empirical experiences,thematic expert discussions,and consultation with an external panel to reach consensus.Results:The BRIDGE framework outlines 6 core components for ICWM-EHR database development:Overall design,database architecture,data extraction and linkage,data governance,data verification,and data quality evaluation.Key data elements include variables related to study population,treatment or exposure,outcomes,and confounders.These databases support various research applications,particularly in evaluating the effectiveness and safety of integrative therapies.To demonstrate its practical value,we developed an ICWM-EHR database on women’s reproductive lifespan,encompassing 2,064,482 patients.This database captures women’s health conditions across the life course,from reproductive age to older adulthood.Conclusions:The BRIDGE methodological framework provides a standardized approach to building high-quality ICWM-EHR databases.It offers a unique opportunity to strengthen the methodological rigor and real-world relevance of Chinese medicine research in integrated healthcare settings.展开更多
Capillary electrophoresis-mass spectrometry（CE-MS） is a powerful separation and analytical technique in the field of analytical chemistry. This review provides an update of instrumentation developments in the method...Capillary electrophoresis-mass spectrometry（CE-MS） is a powerful separation and analytical technique in the field of analytical chemistry. This review provides an update of instrumentation developments in the methodology of CE-MS systems. A selection of relevant articles covers the literatures published from Jan. 2013 to Feb. 2017. Special attentions were paid to the sample injection and ionization processes.Applications of these CE-MS systems were also introduced through representative examples. General conclusions and perspectives were given at the last.展开更多
Requirement engineering in any software development is the most important phase to ensure the success or failure of software. Knowledge modeling and management are helping tools to learn the software organizations. Th...Requirement engineering in any software development is the most important phase to ensure the success or failure of software. Knowledge modeling and management are helping tools to learn the software organizations. The traditional Requirements engineering practices are based upon the interaction of stakeholders which causes iteratively changes in requirements and difficulties in communication and understanding problem domain etc. So, to resolve such issues we use knowledge based techniques to support the RE practices as well as software development process. Our technique is based on two prospective, theoretical and practical implementations. In this paper, we described the need of knowledge management in software engineering and then proposed a model based on knowledge management to support the software development process. To verify our results, we used controlled experiment approach. We have implemented our model, and verify results by using and without using proposed knowledge based RE process. Our resultant proposed model can save the overall cost and time of requirement engineering process as well as software development.展开更多
The work that follows aims at evaluating the urban area/neighborhood of“Agios Konstantinos”in the city of Larissa to the extent that it meets the criteria of green-sustainable design,as these criteria are included i...The work that follows aims at evaluating the urban area/neighborhood of“Agios Konstantinos”in the city of Larissa to the extent that it meets the criteria of green-sustainable design,as these criteria are included in the LEED-Neighborhood Development system methodology.The above methodology encodes the most important elements for green urban planning and provides a quantitative assessment system with key axes those of“smart location and connectivity”,“neighborhood model and plan”and“green infrastructure and buildings”.Nowadays,there is an ongoing debate on urban space and its problems.All urban elements,such as those of the built-structured environment,its open-public areas and its natural elements formulate the urban plot.At the same time,human activities greatly affect both the plot and the living conditions.Environmental issues are often disregarded in urban space and its planning process with damaging effects on cities and,more general,on urban settlements.The advantages and benefits of formally including environmental considerations in urban planning and integrating them into urban development strategies are many,as two of the most important“urban problems”are those of the gradual deterioration and degradation of the area’s microclima and the excessive consumption of energy resources.展开更多
Sustainable development indicators, aim to measure sustainable development over longer periods of time. The sustainable development indicators are grouped into 10 subject categories： socioeconomic development, sustai...Sustainable development indicators, aim to measure sustainable development over longer periods of time. The sustainable development indicators are grouped into 10 subject categories： socioeconomic development, sustainable consumption and production, social inclusion, demographic changes, public health, climate change and energy, sustainable transport, natural resources, global partnership and finally good governance. In this paper, the authors examined a number of researches and compared the methods they used in order to determine the advantages and disadvantages of each method. The methods used a large number of different indicators. The comparison of the indicators could be made through mathematical models and also through theoretical approach.展开更多
The emerging Model-Driven Engineering (MDE) paradigm advocates the use of models as first-class citizens in the software development process, while artifacts such as documentation and source-code can be quickly produc...The emerging Model-Driven Engineering (MDE) paradigm advocates the use of models as first-class citizens in the software development process, while artifacts such as documentation and source-code can be quickly produced from those models by using automated transformations. Even though many MDE-oriented approaches, languages and tools have been developed in the recent past, there is no standard that concretely defines a specific sequence of steps to obtain a functional software system from a model. Thus, the existing approaches present numerous differences among themselves, because each one handles the problems inherent to software development in its own way. This paper presents and discusses a reference model for the comparative study of current MDE approaches in the scope of web-application development. This reference model focuses on relevant aspects such as modeling language scope (domain, business-logic, user-interface), usage of patterns, separation of concerns, model transformations, tool support, and deployment details like web-platform independence and traditional programming required. The ultimate goal of this paper is to determine the aspects that will be of greater importance in future web-oriented MDE languages.展开更多
This paper examines the potential contribution of the fisheries sector to national economic development in Sierra Leone, following the cessation of the recent Ebola virus disease (EVD). The tragic deaths of some 4000 ...This paper examines the potential contribution of the fisheries sector to national economic development in Sierra Leone, following the cessation of the recent Ebola virus disease (EVD). The tragic deaths of some 4000 persons, as a direct result of this epidemic, have also been accompanied by serious economic disruption to one of the world’s poorest countries, amounting to a loss of over USD 1 billion (20 per cent of annual Gross Domestic Product, GDP). Based on recent work undertaken by the New Partnership for Africa’s Development (NEPAD), an assessment was made of the potential contribution of one of the country’s major natural resource sectors—fisheries—to future economic recovery. It was confirmed that the fish resources of Sierra Leone have an estimated capitalised economic value of USD 735 million, and could potentially make an increased contribution to GDP under suitable conditions, over and above the current estimated level of 10 per cent. However, our results also show that with an overall “readiness assessment” score of 41/100, the sector is unlikely to realise its full potential unless a number of specific areas are addressed (based on analysis valid up to January 2016). These include an improvement in fisheries economic assessment, the upgrading of the fisheries governance and management framework, and the establishment of a fisheries development strategy and implementation plan to channel future investments and interventions in an appropriate sequence. Particular attention should be given early on to an increase in the quality and capacity of both public and private sector organisations relevant to roles and functions within a general process of fisheries sector reform. Finally, the fisheries policy evaluation approach used here is novel. While it has been applied to Sierra Leone for the intrinsic interest of that case, this demonstration also shows that it could be applied elsewhere in the future when reviewing fisheries policy.展开更多
文摘In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementing robust security measures in modern web applications and the proof of the Methodology applied to Vue.js,Spring Boot,and MySQL architecture.The proposed approach addresses critical security challenges through a multi-layered framework that encompasses essential security dimensions including multi-factor authentication,fine-grained authorization controls,sophisticated session management,data confidentiality and integrity protection,secure logging mechanisms,comprehensive error handling,high availability strategies,advanced input validation,and security headers implementation.Significant contributions are made to the field of web application security.First,a detailed catalogue of security requirements specifically tailored to protect web applications against contemporary threats,backed by rigorous analysis and industry best practices.Second,the methodology is validated through a carefully designed proof-of-concept implementation in a controlled environment,demonstrating the practical effectiveness of the security measures.The validation process employs cutting-edge static and dynamic analysis tools for comprehensive dependency validation and vulnerability detection,ensuring robust security coverage.The validation results confirm the prevention and avoidance of security vulnerabilities of the methodology.A key innovation of this work is the seamless integration of DevSecOps practices throughout the secure Software Development Life Cycle(SSDLC),creating a security-first mindset from initial design to deployment.By combining proactive secure coding practices with defensive security approaches,a framework is established that not only strengthens application security but also fosters a culture of security awareness within development teams.This hybrid approach ensures that security considerations are woven into every aspect of the development process,rather than being treated as an afterthought.
基金supported by National Natural Science Foundation of China (32172747 and 32425052)
文摘Background Early embryo development plays a pivotal role in determining pregnancy outcomes,postnatal development,and lifelong health.Therefore,the strategic selection of functional nutrients to enhance embryo development is of paramount importance.In this study,we established a stable porcine trophectoderm cell line expressing dual fluorescent reporter genes driven by the CDX2 and TEAD4 gene promoter segments using lentiviral transfection.Results Three amino acid metabolites—kynurenic acid,taurine,and tryptamine—met the minimum z-score criteria of 2.0 for both luciferase and Renilla luciferase activities and were initially identified as potential metabolites for embryo development,with their beneficial effects validated by qPCR.Given that the identified metabolites are closely related to methionine,arginine,and tryptophan,we selected these three amino acids,using lysine as a standard,and employed response surface methodology combined with our high-throughput screening cell model to efficiently screen and optimize amino acid combination conducive to early embryo development.The optimized candidate amino acid system included lysine(1.87 mmol/L),methionine(0.82 mmol/L),tryptophan(0.23 mmol/L),and arginine(3 mmol/L),with the ratio of 1:0.43:0.12:1.60.In vitro experiments confirmed that this amino acid system enhances the expression of key genes involved in early embryonic development and improves in vitro embryo adhesion.Transcriptomic analysis of blastocysts suggested that candidate amino acid system enhances early embryo development by regulating early embryonic cell cycle and differentiation,as well as improving nutrient absorption.Furthermore,based on response surface methodology,400 sows were used to verify this amino acid system,substituting arginine with the more cost-effective N-carbamoyl glutamate(NCG),a precursor of arginine.The optimal dietary amino acid requirement was predicted to be 0.71%lysine,0.32%methionine,0.22%tryptophan,and 0.10%NCG for sows during early gestation.The optimized amino acid system ratio of the feed,derived from the peripheral release of essential amino acids,was found to be 1:0.45:0.13,which is largely consistent with the results obtained from the cell model optimization.Subsequently,we furtherly verified that this optimal dietary amino acid system significantly increased total litter size,live litter size and litter weight in sows.Conclusions In summary,we successfully established a dual-fluorescent high-throughput screening cell model for the efficient identification of potential nutrients that would promote embryo development and implantation.This innovative approach overcomes the limitations of traditional amino acid nutrition studies in sows,providing a more effective model for enhancing reproductive outcomes.
文摘Product Master Model(PMM)Technology is a philosophy and methodology for organizing,managing and controlling product development processes,sharing the information and knowledge of the product and its processes.In this paper,the concept of PMM is proposes,the basic theory of PMM technologies is studied,the blackboard architecture of PMM is proposed,the product development process framework is explored,and the prototype system of PMM is developed.
文摘This white paper explores three popular development methodologies for network softwarization: DevOps, NetOps, and Verification. The paper compares and contrasts the strengths and weaknesses of each approach and provides recommendations for organizations looking to adopt network softwarization.
基金supported by the National Key Research&Development Program of China(No.2024YFC3505800)the National Natural Science Foundation of China(Nos.82474334,82474335 and 72174132)+3 种基金National Science Fund for Distinguished Young Scholars(No.82225049)the Key Research&Development Projects of Sichuan Provincial Department of Science and Technology(Nos.2024YFFK0174 and 2024YFFK0152)1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(Nos.ZYYC24010 and ZYGD23004)the Special Fund for Traditional Chinese Medicine of Sichuan Provincial Administration of Traditional Chinese Medicine(No.2024zd023).
文摘Objectives:Electronic health records(EHRs)offer valuable real-world data(RWD)for Chinese medicine research.However,significant methodological challenges remain in developing integrative Chinese-Western medicine(ICWM)databases.This study aims to establish a best-practice methodological framework,referred to as BRIDGE,to guide the construction of ICWM databases using EHRs.Methods:We developed the methodological framework through a comprehensive process,including systematic literature review,synthesis of empirical experiences,thematic expert discussions,and consultation with an external panel to reach consensus.Results:The BRIDGE framework outlines 6 core components for ICWM-EHR database development:Overall design,database architecture,data extraction and linkage,data governance,data verification,and data quality evaluation.Key data elements include variables related to study population,treatment or exposure,outcomes,and confounders.These databases support various research applications,particularly in evaluating the effectiveness and safety of integrative therapies.To demonstrate its practical value,we developed an ICWM-EHR database on women’s reproductive lifespan,encompassing 2,064,482 patients.This database captures women’s health conditions across the life course,from reproductive age to older adulthood.Conclusions:The BRIDGE methodological framework provides a standardized approach to building high-quality ICWM-EHR databases.It offers a unique opportunity to strengthen the methodological rigor and real-world relevance of Chinese medicine research in integrated healthcare settings.
基金supported by Ministry of Science and Technology(MOST)instrumentation program of China(No.2012YQ04014007)National Natural Science Foundation of China(NSF)(No.21475010)+1 种基金Beijing Natural Science Foundation(BNSF)(No.16L00065)State Key Laboratory Explosion Science and Technology(No.YBKT16-17)
文摘Capillary electrophoresis-mass spectrometry（CE-MS） is a powerful separation and analytical technique in the field of analytical chemistry. This review provides an update of instrumentation developments in the methodology of CE-MS systems. A selection of relevant articles covers the literatures published from Jan. 2013 to Feb. 2017. Special attentions were paid to the sample injection and ionization processes.Applications of these CE-MS systems were also introduced through representative examples. General conclusions and perspectives were given at the last.
文摘Requirement engineering in any software development is the most important phase to ensure the success or failure of software. Knowledge modeling and management are helping tools to learn the software organizations. The traditional Requirements engineering practices are based upon the interaction of stakeholders which causes iteratively changes in requirements and difficulties in communication and understanding problem domain etc. So, to resolve such issues we use knowledge based techniques to support the RE practices as well as software development process. Our technique is based on two prospective, theoretical and practical implementations. In this paper, we described the need of knowledge management in software engineering and then proposed a model based on knowledge management to support the software development process. To verify our results, we used controlled experiment approach. We have implemented our model, and verify results by using and without using proposed knowledge based RE process. Our resultant proposed model can save the overall cost and time of requirement engineering process as well as software development.
文摘The work that follows aims at evaluating the urban area/neighborhood of“Agios Konstantinos”in the city of Larissa to the extent that it meets the criteria of green-sustainable design,as these criteria are included in the LEED-Neighborhood Development system methodology.The above methodology encodes the most important elements for green urban planning and provides a quantitative assessment system with key axes those of“smart location and connectivity”,“neighborhood model and plan”and“green infrastructure and buildings”.Nowadays,there is an ongoing debate on urban space and its problems.All urban elements,such as those of the built-structured environment,its open-public areas and its natural elements formulate the urban plot.At the same time,human activities greatly affect both the plot and the living conditions.Environmental issues are often disregarded in urban space and its planning process with damaging effects on cities and,more general,on urban settlements.The advantages and benefits of formally including environmental considerations in urban planning and integrating them into urban development strategies are many,as two of the most important“urban problems”are those of the gradual deterioration and degradation of the area’s microclima and the excessive consumption of energy resources.
文摘Sustainable development indicators, aim to measure sustainable development over longer periods of time. The sustainable development indicators are grouped into 10 subject categories： socioeconomic development, sustainable consumption and production, social inclusion, demographic changes, public health, climate change and energy, sustainable transport, natural resources, global partnership and finally good governance. In this paper, the authors examined a number of researches and compared the methods they used in order to determine the advantages and disadvantages of each method. The methods used a large number of different indicators. The comparison of the indicators could be made through mathematical models and also through theoretical approach.
文摘The emerging Model-Driven Engineering (MDE) paradigm advocates the use of models as first-class citizens in the software development process, while artifacts such as documentation and source-code can be quickly produced from those models by using automated transformations. Even though many MDE-oriented approaches, languages and tools have been developed in the recent past, there is no standard that concretely defines a specific sequence of steps to obtain a functional software system from a model. Thus, the existing approaches present numerous differences among themselves, because each one handles the problems inherent to software development in its own way. This paper presents and discusses a reference model for the comparative study of current MDE approaches in the scope of web-application development. This reference model focuses on relevant aspects such as modeling language scope (domain, business-logic, user-interface), usage of patterns, separation of concerns, model transformations, tool support, and deployment details like web-platform independence and traditional programming required. The ultimate goal of this paper is to determine the aspects that will be of greater importance in future web-oriented MDE languages.
文摘This paper examines the potential contribution of the fisheries sector to national economic development in Sierra Leone, following the cessation of the recent Ebola virus disease (EVD). The tragic deaths of some 4000 persons, as a direct result of this epidemic, have also been accompanied by serious economic disruption to one of the world’s poorest countries, amounting to a loss of over USD 1 billion (20 per cent of annual Gross Domestic Product, GDP). Based on recent work undertaken by the New Partnership for Africa’s Development (NEPAD), an assessment was made of the potential contribution of one of the country’s major natural resource sectors—fisheries—to future economic recovery. It was confirmed that the fish resources of Sierra Leone have an estimated capitalised economic value of USD 735 million, and could potentially make an increased contribution to GDP under suitable conditions, over and above the current estimated level of 10 per cent. However, our results also show that with an overall “readiness assessment” score of 41/100, the sector is unlikely to realise its full potential unless a number of specific areas are addressed (based on analysis valid up to January 2016). These include an improvement in fisheries economic assessment, the upgrading of the fisheries governance and management framework, and the establishment of a fisheries development strategy and implementation plan to channel future investments and interventions in an appropriate sequence. Particular attention should be given early on to an increase in the quality and capacity of both public and private sector organisations relevant to roles and functions within a general process of fisheries sector reform. Finally, the fisheries policy evaluation approach used here is novel. While it has been applied to Sierra Leone for the intrinsic interest of that case, this demonstration also shows that it could be applied elsewhere in the future when reviewing fisheries policy.
