Processes supported by process-aware information systems are subject to continuous and often subtle changes due to evolving operational,organizational,or regulatory factors.These changes,referred to as incremental con...Processes supported by process-aware information systems are subject to continuous and often subtle changes due to evolving operational,organizational,or regulatory factors.These changes,referred to as incremental concept drift,gradually alter the behavior or structure of processes,making their detection and localization a challenging task.Traditional process mining techniques frequently assume process stationarity and are limited in their ability to detect such drift,particularly from a control-flow perspective.The objective of this research is to develop an interpretable and robust framework capable of detecting and localizing incremental concept drift in event logs,with a specific emphasis on the structural evolution of control-flow semantics in processes.We propose DriftXMiner,a control-flow-aware hybrid framework that combines statistical,machine learning,and process model analysis techniques.The approach comprises three key components:(1)Cumulative Drift Scanner that tracks directional statistical deviations to detect early drift signals;(2)a Temporal Clustering and Drift-Aware Forest Ensemble(DAFE)to capture distributional and classification-level changes in process behavior;and(3)Petri net-based process model reconstruction,which enables the precise localization of structural drift using transition deviation metrics and replay fitness scores.Experimental validation on the BPI Challenge 2017 event log demonstrates that DriftXMiner effectively identifies and localizes gradual and incremental process drift over time.The framework achieves a detection accuracy of 92.5%,a localization precision of 90.3%,and an F1-score of 0.91,outperforming competitive baselines such as CUSUM+Histograms and ADWIN+Alpha Miner.Visual analyses further confirm that identified drift points align with transitions in control-flow models and behavioral cluster structures.DriftXMiner offers a novel and interpretable solution for incremental concept drift detection and localization in dynamic,process-aware systems.By integrating statistical signal accumulation,temporal behavior profiling,and structural process mining,the framework enables finegrained drift explanation and supports adaptive process intelligence in evolving environments.Its modular architecture supports extension to streaming data and real-time monitoring contexts.展开更多
Deep Underground Science and Engineering(DUSE)is pleased to present this special issue on Groundwater and Stability in Deep Mining.As mining operations progress to greater depths to meet the growing global demand for ...Deep Underground Science and Engineering(DUSE)is pleased to present this special issue on Groundwater and Stability in Deep Mining.As mining operations progress to greater depths to meet the growing global demand for mineral resources and energy,the challenges associated with groundwater control and rock mass stability have grown increasingly critical.These challenges are exacerbated by complex geological conditions,structural heterogeneity,and intense mining-induced disturbances.This special issue seeks to address these challenges by showcasing cutting-edge research and technological advancements in the field.展开更多
Based on the characteristics of strata movement of solid backfilling mining technology, the surface subsidence prediction method based on the equivalent mining height theory was proposed, and the parameters selection ...Based on the characteristics of strata movement of solid backfilling mining technology, the surface subsidence prediction method based on the equivalent mining height theory was proposed, and the parameters selection guideline of this method was also described. While comparing the parameters of caving mining with equivalent height, the subsidence efficient can be calculated according to the mining height and bulk factor of sagging zone and fracture zone, the tangent of main influence angle of solid backfilling mining is reduced by 0.2-0.5（while it cannot be less than 1.0）. For sake of safety, offset of the inflection point is set to zero, and other parameters, such as horizontal movement coefficient and main propagation angle are equal to the corresponding parameters of caving mining with equivalent height. In the last part, a case study of solid backfilling mining subsidence prediction was described. The results show the applicability of this method and the difference of the maximum subsidence point between the prediction and the observation is less than 5%.展开更多
Taking the test stopes during continuous mining induced roof caving of Tongkeng ore-body No.92 as example, the calculation flow of unloading analysis was established. According to the unloading region division method ...Taking the test stopes during continuous mining induced roof caving of Tongkeng ore-body No.92 as example, the calculation flow of unloading analysis was established. According to the unloading region division method of the affected zone theory, and the deterioration laws of mechanics parameters of unloading rock mass, the continuous mining process in underground mine was analyzed by the software MIDAS/GTS, the mechanical response of roof rock mass unloading was studied, and the differences were analyzed with the conventional simulation. The result shows that the maximum tensile stress, subsidence displacement and equivalent plastic strain of roof rock mass are 1.5 MPa, 20 cm and 1.5% in the unloading analysis, while 1.0 MPa, 13 cm and 0.9% in the conventional analysis. The values of unloading analysis, which are also closer to the actual situation, are greater than those of conventional analysis; the maximum step in continuous mining is 48 m, which shows that the induced treatment of the roof should be carried out after 2 mining steps展开更多
Using electric motors instead of diesel engines as the driving system for mining excavators can reduce the energy consumption and operating costs.However,pure electric-driven mining excavators are prone to unexpected ...Using electric motors instead of diesel engines as the driving system for mining excavators can reduce the energy consumption and operating costs.However,pure electric-driven mining excavators are prone to unexpected power outages in mines because of drastic changes in load power,leading to significant fluctuations in the power demand of the grid,which in turn affects production.To solve the above problem,a pure electric-driven mining hydraulic excavator based on electric-motor-driven swing platform and hydraulic pumps was used as the research object.Moreover,supercapacitors and DC/DC converter,as the energy storage system(ESS)adjust the output power of the grid and recover the braking kinetic energy of the swing platform.Subsequently,a novel integrated energy management strategy for a DC bus voltage predictive controller based on the power feedforward of fuzzy rules is proposed to run mining excavators efficiently and reliably.Specifically,the working modes of the ESS are determined by the DC bus voltage and state of charge(SOC)of the supercapacitor.Next,the output power of the supercapacitor and the DC bus voltage were controlled by adjusting the charging and discharging currents of the DC/DC converter using a predictive controller and fuzzy rules.In addition,a digital prototype of the excavator was verified using an original machine test.The performance of the different strategies and driven systems were analyzed using digital prototypes.The results showed that,compared with traditional excavators with diesel engines,the operational cost of the developed excavators was reduced by 54.02%.Compared to pure electric-driven excavators without an ESS,the peak power of the grid for the developed excavators was reduced by 10%.This study designed an integrated energy management strategy for a pure electric mining excavator that can regulate the power output of the grid and maintain the stability of the bus voltage and SOC of the ESS.展开更多
Shear strain energy is a pivotal physical quantity in the occurrence of earthquakes and rockbursts during deep mining operations.This research is focused on understanding the changes in shear strain energy in the cont...Shear strain energy is a pivotal physical quantity in the occurrence of earthquakes and rockbursts during deep mining operations.This research is focused on understanding the changes in shear strain energy in the context of retreating longwall mining,which is essential for the optimized design and mitigation of rockbursts and seismic events.Through the application of innovative analytical models,this study expands its analytical range to include the variations in shear strain energy caused by fault coseismic slip.An integrated methodology is utilized,taking into account the changes in coseismic and fault friction parameters as well as enhancements in mining-induced stress and existing background stresses.Our numerical investigation highlights the significance of mining location and fault characteristics as key determinants of shear strain energy modifications.The analysis demonstrates significant spatial variability in shear strain energy,especially noting that fault slip near the mining face greatly increases the likelihood of rockburst.This finding emphasizes the need to integrate fault coseismic slip dynamics into the triggering factors of rock(coal)bursts,thus broadening the theoretical foundation for addressing geological hazards in deep mining operations.The results are further corroborated by observational data from the vicinity of the F16 fault zone,introducing the concept of mining-induced fault coseismic slip as an essential element in the theoretical framework for understanding rockburst triggers.展开更多
Deep-sea mining has emerged as a critical solution to address global resource shortages;however,the mechanical interaction between tracked mining vehicles(TMVs)and soft seabed sediments presents fundamental engineerin...Deep-sea mining has emerged as a critical solution to address global resource shortages;however,the mechanical interaction between tracked mining vehicles(TMVs)and soft seabed sediments presents fundamental engineering challenges.This study establishes a multiscale modelling framework coupling the discrete element method(DEM)with multi-body dynamics(MBD)to investigate track-seabed dynamic interactions across three operational modes:flat terrain,slope climbing,and ditch surmounting.The simulation framework,validated against laboratory experiments,systematically evaluates the influence of grouser geometry(involute,triangular,and pin-type)and traveling speed(0.2–1.0 m/s)on traction performance,slip rate,and ground pressure distribution.Results reveal rate-dependent traction mechanisms governed by soil microstructural responses:higher speeds enhance peak traction but exacerbate slip instability on complex terrain.Critical operational thresholds are established—0.7 m/s for flat terrain,≤0.5 m/s for slopes and ditches—with distinct grouser optimization strategies:involute grousers achieve 35%–40%slip reduction on slopes through progressive soil engagement,while triangular grousers provide optimal impact resistance during ditch crossing with 30%–35%performance improvement.These findings provide quantitative design criteria and operational guidelines for optimizing TMV structural parameters and control strategies,offering a robust theoretical foundation for enhancing the performance,safety,and reliability of deep-sea mining equipment in complex submarine environments.展开更多
Rising demand for minerals and metals in high-tech and new energy industries has led to a great interest in exploration of seabed mineral resources.Such resources,including polymetallic nodule(PMN),polymetallic sulphi...Rising demand for minerals and metals in high-tech and new energy industries has led to a great interest in exploration of seabed mineral resources.Such resources,including polymetallic nodule(PMN),polymetallic sulphide(PMS),and cobalt-rich ferromanganese crust(CFC),are considered as an alternative source of metals to terrestrial deposits.Although a considerable number of sea trials of deep-sea mining have been carried out,the deep-sea mining does not achieve the commercial exploitation due to the complexity of deep-sea mining system and deep-sea mining environment.In fact,to achieve commercial deep-sea mining,the technology and equipment of deep-sea mining are the key points.Therefore,the present study presents the development of the technology and equipment of deep-sea mining.It commences with a requirement of technology and equipment for deep-sea mining,including environmental impact,reliability,energy cost,efficiency,etc.Then,a historical perspective and present-day effort related to deep-sea mining vehicles are given,which highlights the evolution of collection mechanism and walking mode of deep-sea mining vehicle.Subsequently,the present study discusses the operation of subsea lifting system and surface support system,shedding light on the crucial equipment and processes.The challenges and prospects in the deep-sea mining are presented in final,including environmental protection,self-propelled crawler,hydraulic pipeline lifting,and intelligent equipment,etc.展开更多
An application programming interface (API) usage specifcation, which includes the conditions, calling sequences, and semantic relationships of the API, is important for verifying its correct usage, which is in turn cr...An application programming interface (API) usage specifcation, which includes the conditions, calling sequences, and semantic relationships of the API, is important for verifying its correct usage, which is in turn critical for ensur-ingthe security and availability of the target program. However, existing techniques either mine the co-occurring relationships of multiple APIs without considering their semantic relationships, or they use data fow and control fow information to extract semantic beliefs on API pairs but difcult to incorporate when mining specifcations for mul-tipleAPIs. Hence, we propose an API specifcation mining approach that efciently extracts a relatively complete list of the API combinations and semantic relationships between APIs. This approach analyzes a target program in two stages. The frst stage uses frequent API set mining based on frequent common API identifcation and fltra-tionto extract the maximal set of frequent context-sensitive API sequences. In the second stage, the API relationship graph is constructed using three semantic relationships extracted from the symbolic path information, and the speci-fcationscontaining semantic relationships for multiple APIs are mined. The experimental results on six popular open-source code bases of diferent scales show that the proposed two-stage approach not only yields better results than existing typical approaches, but also can efectively discover the specifcations along with the semantic rela-tionshipsfor multiple APIs. Instance analysis shows that the analysis of security-related API call violations can assist in the cause analysis and patch of software vulnerabilities.展开更多
To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and ...To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and Ni in China’s Dexing copper mining district through paired sampling of water-amphibians,soil-earthworms,and air-lichens.Advanced methodologies were employed,including ICP-MS quantification for heavy metals,geochemical indices(Igeo,BCF,BAF)to assess bioavailability,NMDS for source apportionment,and HPLC to detect DNA methylation alterations.Aquatic systems exhibited severe Cd/Pb enrichment(16.25-24.42μg/L;11-15×WHO limits),while agricultural soils showed extreme Cd contamination(1.5 mg/kg;15×background).Biota displayed metal-specific accumulation:frogs achieved BCFs>1,000 for Pb/Cd,earthworms showed pH-modulated BAFs>2.5 for Cd/As,and lichens recorded 100-1,000×atmospheric Cr enrichment.NMDS resolved three contamination pathways:mining-derived Cd/Pb/As(MDS1=2.56),atmospheric Cr(PC2=1.84),and geogenic Ni.Cd dominated ecological risks(Eri=554.25;RI 300),while atmospheric Cr drove carcinogenic risks(TCR=4.11×10^(-5))exceeding safety thresholds.The source-media-biota-risk framework pioneers the integration of geochemical transport with epigenetic toxicity biomarkers,demonstrating that sub-lethal Cd/Pb exposure induces genome-wide DNA hypomethylation(2.4%-6.6%reduction;ρ=−0.71 to−0.91).This paradigm shift prioritizes bioavailability-informed regulations over concentration-based metrics,offering actionable strategies for sustainable development goals-aligned mining pollution control.展开更多
With the third innovation in science and technology worldwide, China has also experienced thismarvelous progress. Concerning the longwall mining in China, the ＂masonry beam theory＂ （MBT） wasfirst proposed in the 1...With the third innovation in science and technology worldwide, China has also experienced thismarvelous progress. Concerning the longwall mining in China, the ＂masonry beam theory＂ （MBT） wasfirst proposed in the 1960s, illustrating that the transmission and equilibrium method of overburdenpressure using reserved coal pillar in mined-out areas can be realized. This forms the so-called ＂121mining method＂, which lays a solid foundation for development of mining science and technology inChina. The ＂transfer rock beam theory＂ （TRBT） proposed in the 1980s gives a further understanding forthe transmission path of stope overburden pressure and pressure distribution in high-stress areas. In thisregard, the advanced 121 mining method was proposed with smaller coal pillar for excavation design,making significant contributions to improvement of the coal recovery rate in that era. In the 21st century,the traditional mining technologies faced great challenges and, under the theoretical developmentspioneered by Profs. Minggao Qian and Zhenqi Song, the ＂cutting cantilever beam theory＂ （CCBT） wasproposed in 2008. After that the 110 mining method is formulated subsequently, namely one stope face,after the first mining cycle, needs one advanced gateway excavation, while the other one is automaticallyformed during the last mining cycle without coal pillars left in the mining area. This method can beimplemented using the CCBT by incorporating the key technologies, including the directional presplittingroof cutting, constant resistance and large deformation （CRLD） bolt/anchor supporting systemwith negative Poisson＇s ratio （NPR） effect material, and remote real-time monitoring technology. TheCCBT and 110 mining method will provide the theoretical and technical basis for the development ofmining industry in China.展开更多
In this study, the spatial distributions of stress and fracture fields for three typical underground coal mining layouts, Le, non-pillar mining （NM）, top-coal caving mining （TCM） and protective coal-seam mining （...In this study, the spatial distributions of stress and fracture fields for three typical underground coal mining layouts, Le, non-pillar mining （NM）, top-coal caving mining （TCM） and protective coal-seam mining （PCM）, are modeled using discrete element software UDEC, The numerical results show that different mining layouts can lead to different mining-induced stress fields, resulting in diverse fracture fields, For the PCM, the mining influenced area in front of the mining faces is the largest, and the stress concentration factor in front of the mining faces is the lowest, The spatial shapes of the mining-induced fracture fields under NM, TCM and PCM differ, and they are characterized by trapezoidal, triangular and tower shapes, respectively, The fractal dimensions of mining-induced fractures of the three mining layouts decrease in the order of PCM, TCM and NM, It is also shown that the PCM can result in a better gas control effect in coal mines with high outburst potential, The numerical results are expected to provide a basis for understanding of mining-induced gas seepage fields and provide a reference for high- efficiency coal mining,展开更多
The rehabilitation of coal mines is an up-to-date issue, heavily discussed not only by the industry but also by scientists and the adjacent communities since open cut coal mining has extensive impacts affecting all pa...The rehabilitation of coal mines is an up-to-date issue, heavily discussed not only by the industry but also by scientists and the adjacent communities since open cut coal mining has extensive impacts affecting all parties. Whereas, before and during the mining process not only nature but sometimes also the population of a whole region has to give way to a mine, the consequences of the operations remain long after mining closure. Typically a once intact and functioning ecosystem is replaced with a destroyed wideness evocative of a moon landscape. Fortunately, the awareness of the need to rehabilitate the destroyed nature is increasing steadily and all involved parties are making efforts to improve the situation, even if it is a major challenge to bring all parties to a consensus. Therefore, a legislative basis is required and the know-how of optimal rehabilitation solutions must be developed. In this article the rehabilitation of mined land in Australia and Germany are compared. More precisely the article will present an overview of the legislative regulations, certain applied methods of rehabilitation work and the problems that are likely to occur in respect to the influence of mining on nature.展开更多
1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrest...1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrestrial ecosystems.展开更多
A deep-sea mining riser is a crucial component of the system used to lift seafloor mineral resources to the vessel.It is prone to damage and failure because of harsh environmental conditions and internal fluid erosion...A deep-sea mining riser is a crucial component of the system used to lift seafloor mineral resources to the vessel.It is prone to damage and failure because of harsh environmental conditions and internal fluid erosion.Furthermore,damage can impact the response characteristics of the riser,but varying environmental loadings easily mask it.Thus,distin-guishing between riser damage and environmental effects poses a considerable challenge.To address this issue,a cantilevered model is created for a deep-sea mining riser via the concentrated mass method,and a time-domain analytical strategy is developed.The vortex-induced vibration(VIV)response characteristics of the riser are initially examined,considering various damage conditions and flow velocities.The study results revealed four primary observations:(a)effective tension can serve as a reliable indicator for identifying damage at lower velocities;(b)there are noticeable differences in displacement between the healthy and damaged risers in the in-line direction rather than the cross-flow direction;(c)frequency characteristics can more effectively distinguish the damage conditions at high flow velocities,with the mean square frequency and frequency variance being more effective than the centroid frequency and root variance frequency;(d)displacement differences are more sensitive to damage occurring near the top and bottom of the riser,while both velocity variations and structural damage can influence displacements,especially in regions between modal nodes.The vibrational behavior and damage indicators are clarified for structural health monitoring of deep-sea mining risers during lifting operations.展开更多
Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base ...Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base in China with significant strategic importance,has undergone intensive coal mining activities that have substantially disrupted regional groundwater circulation.This study integrated data from the Gravity Recovery and Climate Experiment Satellite(GRACE)and Famine Early Warning Systems Network(FEWS NET)Land Data Assimilation System(FLDAS)models,combined with weighted downscaling methodology and water balance principles,to reconstruct high-resolution(0.01°)terrestrial water storage(TWS)and GWS changes in the Ordos Mining Region,China from April 2002 to December 2021.The accuracy of GWS variations were validated through pumping test measurements.Subsequently,Geodetector analysis was implemented to quantify the contributions of natural and anthropogenic factors to groundwater storage dynamics.Key findings include:1)TWS in the study area showed a fluctuating but overall decreasing trend,with a total reduction of 8901.11 mm during study period.The most significant annual decrease occurred in 2021,reaching 1696.77 mm.2)GWS exhibited an accelerated decline,with an average annual change rate of 44.35 mm/yr,totaling a decrease of 887.05 mm.The lowest annual groundwater storage level was recorded in 2020,reaching 185.69 mm.3)Precipitation(PRE)contributed the most to GWS variation(q=0.52),followed by coal mining water consumption(MWS)(q=0.41).The interaction between PRE and MWS exhibited a nonlinear enhancement effect on GWS changes(0.54).The synergistic effect of natural hydrological factors has a great influence on the change of GWS,but coal mining water consumption will continue to reduce GWS.These findings provide critical references for the management and regulation of groundwater resource in mining regions.展开更多
Copper smelting is the main source of arsenic pollution in the environment,and China is the largest country for copper smelting.Taking 2022 as an example,this study analyzes the distribution and fate of arsenic across...Copper smelting is the main source of arsenic pollution in the environment,and China is the largest country for copper smelting.Taking 2022 as an example,this study analyzes the distribution and fate of arsenic across the copper mining,beneficiation,and smelting processes using a life-cycle approach,providing important insights for arsenic pollution prevention and the resource utilization of arsenic-bearing solid waste.The results show that the amount of As in waste rock,tailing and concentrate are 53483 t,86632 t,76162 t,respectively.After smelting treatment,the amount of arsenic in different types of solid waste,wastewater,waste gas and products are 76128 t,1 t,31 t and 2 t,respectively,and the proportion in arsenic sulfide slag is the highest(55%).The amount of emission to the environment is 32 t,accounting for only 0.04%of total amount.In the future,key considerations are to improve the resource utilization rate of arsenic-containing solid waste(tailing,smelting slag),especially arsenic sulfide slag,and to digest its environmental risk.展开更多
Xinli district of Sanshandao Gold Mine is the first subsea metal mine in China.To achieve 6 kt/d production capacity under the premise of safe mining,high-intensity mining might destroy the in-situ stress filed and th...Xinli district of Sanshandao Gold Mine is the first subsea metal mine in China.To achieve 6 kt/d production capacity under the premise of safe mining,high-intensity mining might destroy the in-situ stress filed and the stability of rockmass.According to sampling and testing of ore-rock and backfill and in-situ stress field measurement,safety factor method calculation model based on stress-strain strength reduction at arbitrary points and Mohr-Coulomb yield criterion was established and limit displacement subsidence values under the safety factor of different limit stoping steps were calculated.The results from three years in-situ mining and strata movement monitoring using multi-point displacements meter showed that the lower settlement frame stope hierarchical level filling mining method,mining sequence are reasonable and rockmass stability evaluation using safety factor method,in-situ real-time monitoring can provide the technical foundation for the safety of seabed mining.展开更多
Enhancing the mining speed of a working face has become the primary approach to achieve high production and efficiency in coal mines,thereby further improving the production capacity.However,the problem of rock bursts...Enhancing the mining speed of a working face has become the primary approach to achieve high production and efficiency in coal mines,thereby further improving the production capacity.However,the problem of rock bursts resulting from this approach has become increasingly serious.Therefore,to implement coal mine safety and efficient extraction,the impact of deformation pressure caused by different mining speeds should be considered,and a reasonable mining speed of the working face should be determined.The influence of mining speed on overlying rock breaking in the stope is analyzed by establishing a key layer block rotation and subsidence model.Results show that with the increasing mining speed,the compression amount of gangue in the goaf decreases,and the rotation and subsidence amount of rock block B above goaf decreases,forcing the rotation and subsidence amount of rock block A above roadway to increase.Consequently,the contact mode between rock block A and rock block B changes from line contact to point contact,and the horizontal thrust and shear force between blocks increase.The increase in rotation and subsidence of rock block A intensifies the compression degree of coal and rock mass below the key layer,thereby increasing the stress concentration degree of coal and rock mass as well as the total energy accumulation.In addition,due to the insufficient compression of gangue in the goaf,the bending and subsidence space of the far-field key layer are limited,the length of the suspended roof increases,and the influence range of mining stress and the energy accumulation range expand.Numerical test results and underground microseismic monitoring results verify the correlation between mining speed and stope energy,and high-energy events generally appear 1-2 d after the change in mining speed.On this basis,the statistical principle confirms that the maximum mining speed of the working face at 6 m/d is reasonable.展开更多
In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids....In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids.Initially,potential endophytic fungi were identified through genome mining.Subsequently,fine fractions containing various fernane-type triterpenoids were selected using HSQC data collection and SMART prediction.These triterpenoids were then obtained through targeted isolation and identification.Finally,their antifungal activity was evaluated.As a result,three fernane-type triterpenoids,including two novel compounds,along with two new sesquiterpenes and four known compounds were isolated from one potential strain,Diaporthe discoidispora.Their structures were elucidated through analysis of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(NMR)spectroscopic data.The absolute configurations were determined using single-crystal X-ray diffraction analysis and electron capture detector(ECD)analysis.Compound 3 exhibited moderate antifungal activity against Candida albicans CMCC 98001 and Aspergillus niger.展开更多
文摘Processes supported by process-aware information systems are subject to continuous and often subtle changes due to evolving operational,organizational,or regulatory factors.These changes,referred to as incremental concept drift,gradually alter the behavior or structure of processes,making their detection and localization a challenging task.Traditional process mining techniques frequently assume process stationarity and are limited in their ability to detect such drift,particularly from a control-flow perspective.The objective of this research is to develop an interpretable and robust framework capable of detecting and localizing incremental concept drift in event logs,with a specific emphasis on the structural evolution of control-flow semantics in processes.We propose DriftXMiner,a control-flow-aware hybrid framework that combines statistical,machine learning,and process model analysis techniques.The approach comprises three key components:(1)Cumulative Drift Scanner that tracks directional statistical deviations to detect early drift signals;(2)a Temporal Clustering and Drift-Aware Forest Ensemble(DAFE)to capture distributional and classification-level changes in process behavior;and(3)Petri net-based process model reconstruction,which enables the precise localization of structural drift using transition deviation metrics and replay fitness scores.Experimental validation on the BPI Challenge 2017 event log demonstrates that DriftXMiner effectively identifies and localizes gradual and incremental process drift over time.The framework achieves a detection accuracy of 92.5%,a localization precision of 90.3%,and an F1-score of 0.91,outperforming competitive baselines such as CUSUM+Histograms and ADWIN+Alpha Miner.Visual analyses further confirm that identified drift points align with transitions in control-flow models and behavioral cluster structures.DriftXMiner offers a novel and interpretable solution for incremental concept drift detection and localization in dynamic,process-aware systems.By integrating statistical signal accumulation,temporal behavior profiling,and structural process mining,the framework enables finegrained drift explanation and supports adaptive process intelligence in evolving environments.Its modular architecture supports extension to streaming data and real-time monitoring contexts.
文摘Deep Underground Science and Engineering(DUSE)is pleased to present this special issue on Groundwater and Stability in Deep Mining.As mining operations progress to greater depths to meet the growing global demand for mineral resources and energy,the challenges associated with groundwater control and rock mass stability have grown increasingly critical.These challenges are exacerbated by complex geological conditions,structural heterogeneity,and intense mining-induced disturbances.This special issue seeks to address these challenges by showcasing cutting-edge research and technological advancements in the field.
基金Project(2012BAB13B03)supported by the National Scientific and Technical Supporting Programs Funded of ChinaProject(41104011)supported by the National Natural Science Foundation of China+1 种基金Project(2013QNB07)supported by the Natural Science Funds for Young Scholar of China University of Mining and TechnologyProject(2012LWB32)supported by the Fundamental Research Funds for the Central Universities,China
文摘Based on the characteristics of strata movement of solid backfilling mining technology, the surface subsidence prediction method based on the equivalent mining height theory was proposed, and the parameters selection guideline of this method was also described. While comparing the parameters of caving mining with equivalent height, the subsidence efficient can be calculated according to the mining height and bulk factor of sagging zone and fracture zone, the tangent of main influence angle of solid backfilling mining is reduced by 0.2-0.5（while it cannot be less than 1.0）. For sake of safety, offset of the inflection point is set to zero, and other parameters, such as horizontal movement coefficient and main propagation angle are equal to the corresponding parameters of caving mining with equivalent height. In the last part, a case study of solid backfilling mining subsidence prediction was described. The results show the applicability of this method and the difference of the maximum subsidence point between the prediction and the observation is less than 5%.
基金Projects (50934006, 51074178) supported by the National Natural Science Foundation of ChinaProject (2010QZZD001) supported by the Fundamental Research Funds for the Central Universities of China
文摘Taking the test stopes during continuous mining induced roof caving of Tongkeng ore-body No.92 as example, the calculation flow of unloading analysis was established. According to the unloading region division method of the affected zone theory, and the deterioration laws of mechanics parameters of unloading rock mass, the continuous mining process in underground mine was analyzed by the software MIDAS/GTS, the mechanical response of roof rock mass unloading was studied, and the differences were analyzed with the conventional simulation. The result shows that the maximum tensile stress, subsidence displacement and equivalent plastic strain of roof rock mass are 1.5 MPa, 20 cm and 1.5% in the unloading analysis, while 1.0 MPa, 13 cm and 0.9% in the conventional analysis. The values of unloading analysis, which are also closer to the actual situation, are greater than those of conventional analysis; the maximum step in continuous mining is 48 m, which shows that the induced treatment of the roof should be carried out after 2 mining steps
基金Supported by National Natural Science Foundation of ChinaShanxi Coalbased Low-Carbon Joint Fund(Grant No.U1910211)。
文摘Using electric motors instead of diesel engines as the driving system for mining excavators can reduce the energy consumption and operating costs.However,pure electric-driven mining excavators are prone to unexpected power outages in mines because of drastic changes in load power,leading to significant fluctuations in the power demand of the grid,which in turn affects production.To solve the above problem,a pure electric-driven mining hydraulic excavator based on electric-motor-driven swing platform and hydraulic pumps was used as the research object.Moreover,supercapacitors and DC/DC converter,as the energy storage system(ESS)adjust the output power of the grid and recover the braking kinetic energy of the swing platform.Subsequently,a novel integrated energy management strategy for a DC bus voltage predictive controller based on the power feedforward of fuzzy rules is proposed to run mining excavators efficiently and reliably.Specifically,the working modes of the ESS are determined by the DC bus voltage and state of charge(SOC)of the supercapacitor.Next,the output power of the supercapacitor and the DC bus voltage were controlled by adjusting the charging and discharging currents of the DC/DC converter using a predictive controller and fuzzy rules.In addition,a digital prototype of the excavator was verified using an original machine test.The performance of the different strategies and driven systems were analyzed using digital prototypes.The results showed that,compared with traditional excavators with diesel engines,the operational cost of the developed excavators was reduced by 54.02%.Compared to pure electric-driven excavators without an ESS,the peak power of the grid for the developed excavators was reduced by 10%.This study designed an integrated energy management strategy for a pure electric mining excavator that can regulate the power output of the grid and maintain the stability of the bus voltage and SOC of the ESS.
文摘Shear strain energy is a pivotal physical quantity in the occurrence of earthquakes and rockbursts during deep mining operations.This research is focused on understanding the changes in shear strain energy in the context of retreating longwall mining,which is essential for the optimized design and mitigation of rockbursts and seismic events.Through the application of innovative analytical models,this study expands its analytical range to include the variations in shear strain energy caused by fault coseismic slip.An integrated methodology is utilized,taking into account the changes in coseismic and fault friction parameters as well as enhancements in mining-induced stress and existing background stresses.Our numerical investigation highlights the significance of mining location and fault characteristics as key determinants of shear strain energy modifications.The analysis demonstrates significant spatial variability in shear strain energy,especially noting that fault slip near the mining face greatly increases the likelihood of rockburst.This finding emphasizes the need to integrate fault coseismic slip dynamics into the triggering factors of rock(coal)bursts,thus broadening the theoretical foundation for addressing geological hazards in deep mining operations.The results are further corroborated by observational data from the vicinity of the F16 fault zone,introducing the concept of mining-induced fault coseismic slip as an essential element in the theoretical framework for understanding rockburst triggers.
基金financially supported by the National Key Research and Development Program of China-Young Scientist Project(No.2024YFC2815400)the National Natural Science Foundation of China(No.52588202).
文摘Deep-sea mining has emerged as a critical solution to address global resource shortages;however,the mechanical interaction between tracked mining vehicles(TMVs)and soft seabed sediments presents fundamental engineering challenges.This study establishes a multiscale modelling framework coupling the discrete element method(DEM)with multi-body dynamics(MBD)to investigate track-seabed dynamic interactions across three operational modes:flat terrain,slope climbing,and ditch surmounting.The simulation framework,validated against laboratory experiments,systematically evaluates the influence of grouser geometry(involute,triangular,and pin-type)and traveling speed(0.2–1.0 m/s)on traction performance,slip rate,and ground pressure distribution.Results reveal rate-dependent traction mechanisms governed by soil microstructural responses:higher speeds enhance peak traction but exacerbate slip instability on complex terrain.Critical operational thresholds are established—0.7 m/s for flat terrain,≤0.5 m/s for slopes and ditches—with distinct grouser optimization strategies:involute grousers achieve 35%–40%slip reduction on slopes through progressive soil engagement,while triangular grousers provide optimal impact resistance during ditch crossing with 30%–35%performance improvement.These findings provide quantitative design criteria and operational guidelines for optimizing TMV structural parameters and control strategies,offering a robust theoretical foundation for enhancing the performance,safety,and reliability of deep-sea mining equipment in complex submarine environments.
基金the National Science Fund for Distinguished Young Scholars(Grant No.52225107)National Key Research and Development Program of China(Grant No.2021YFC2801503)for funding provided to this work.
文摘Rising demand for minerals and metals in high-tech and new energy industries has led to a great interest in exploration of seabed mineral resources.Such resources,including polymetallic nodule(PMN),polymetallic sulphide(PMS),and cobalt-rich ferromanganese crust(CFC),are considered as an alternative source of metals to terrestrial deposits.Although a considerable number of sea trials of deep-sea mining have been carried out,the deep-sea mining does not achieve the commercial exploitation due to the complexity of deep-sea mining system and deep-sea mining environment.In fact,to achieve commercial deep-sea mining,the technology and equipment of deep-sea mining are the key points.Therefore,the present study presents the development of the technology and equipment of deep-sea mining.It commences with a requirement of technology and equipment for deep-sea mining,including environmental impact,reliability,energy cost,efficiency,etc.Then,a historical perspective and present-day effort related to deep-sea mining vehicles are given,which highlights the evolution of collection mechanism and walking mode of deep-sea mining vehicle.Subsequently,the present study discusses the operation of subsea lifting system and surface support system,shedding light on the crucial equipment and processes.The challenges and prospects in the deep-sea mining are presented in final,including environmental protection,self-propelled crawler,hydraulic pipeline lifting,and intelligent equipment,etc.
文摘An application programming interface (API) usage specifcation, which includes the conditions, calling sequences, and semantic relationships of the API, is important for verifying its correct usage, which is in turn critical for ensur-ingthe security and availability of the target program. However, existing techniques either mine the co-occurring relationships of multiple APIs without considering their semantic relationships, or they use data fow and control fow information to extract semantic beliefs on API pairs but difcult to incorporate when mining specifcations for mul-tipleAPIs. Hence, we propose an API specifcation mining approach that efciently extracts a relatively complete list of the API combinations and semantic relationships between APIs. This approach analyzes a target program in two stages. The frst stage uses frequent API set mining based on frequent common API identifcation and fltra-tionto extract the maximal set of frequent context-sensitive API sequences. In the second stage, the API relationship graph is constructed using three semantic relationships extracted from the symbolic path information, and the speci-fcationscontaining semantic relationships for multiple APIs are mined. The experimental results on six popular open-source code bases of diferent scales show that the proposed two-stage approach not only yields better results than existing typical approaches, but also can efectively discover the specifcations along with the semantic rela-tionshipsfor multiple APIs. Instance analysis shows that the analysis of security-related API call violations can assist in the cause analysis and patch of software vulnerabilities.
基金financially supported by the Fundation of Key Laboratory of Ministry of Natural Resources for Eco-geochemistry (ZSDHJJ202202)Geological Investigation and Evaluation of Shale Gas in Complex Structural Areas of the Middle Yangtze plate(DD20250200604) of China Geological Survey+1 种基金the Natural Science Foundation of Guangdong Province,China(2023A1515140061)the Dongguan Science and Technology of Social Development Program(20231800935842, 20231800940562).
文摘To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and Ni in China’s Dexing copper mining district through paired sampling of water-amphibians,soil-earthworms,and air-lichens.Advanced methodologies were employed,including ICP-MS quantification for heavy metals,geochemical indices(Igeo,BCF,BAF)to assess bioavailability,NMDS for source apportionment,and HPLC to detect DNA methylation alterations.Aquatic systems exhibited severe Cd/Pb enrichment(16.25-24.42μg/L;11-15×WHO limits),while agricultural soils showed extreme Cd contamination(1.5 mg/kg;15×background).Biota displayed metal-specific accumulation:frogs achieved BCFs>1,000 for Pb/Cd,earthworms showed pH-modulated BAFs>2.5 for Cd/As,and lichens recorded 100-1,000×atmospheric Cr enrichment.NMDS resolved three contamination pathways:mining-derived Cd/Pb/As(MDS1=2.56),atmospheric Cr(PC2=1.84),and geogenic Ni.Cd dominated ecological risks(Eri=554.25;RI 300),while atmospheric Cr drove carcinogenic risks(TCR=4.11×10^(-5))exceeding safety thresholds.The source-media-biota-risk framework pioneers the integration of geochemical transport with epigenetic toxicity biomarkers,demonstrating that sub-lethal Cd/Pb exposure induces genome-wide DNA hypomethylation(2.4%-6.6%reduction;ρ=−0.71 to−0.91).This paradigm shift prioritizes bioavailability-informed regulations over concentration-based metrics,offering actionable strategies for sustainable development goals-aligned mining pollution control.
基金supported by the National Natural Science Foundation of China (No. 51404278)the State Key Program of National Natural Science Foundation of China (No. 51134005)
文摘With the third innovation in science and technology worldwide, China has also experienced thismarvelous progress. Concerning the longwall mining in China, the ＂masonry beam theory＂ （MBT） wasfirst proposed in the 1960s, illustrating that the transmission and equilibrium method of overburdenpressure using reserved coal pillar in mined-out areas can be realized. This forms the so-called ＂121mining method＂, which lays a solid foundation for development of mining science and technology inChina. The ＂transfer rock beam theory＂ （TRBT） proposed in the 1980s gives a further understanding forthe transmission path of stope overburden pressure and pressure distribution in high-stress areas. In thisregard, the advanced 121 mining method was proposed with smaller coal pillar for excavation design,making significant contributions to improvement of the coal recovery rate in that era. In the 21st century,the traditional mining technologies faced great challenges and, under the theoretical developmentspioneered by Profs. Minggao Qian and Zhenqi Song, the ＂cutting cantilever beam theory＂ （CCBT） wasproposed in 2008. After that the 110 mining method is formulated subsequently, namely one stope face,after the first mining cycle, needs one advanced gateway excavation, while the other one is automaticallyformed during the last mining cycle without coal pillars left in the mining area. This method can beimplemented using the CCBT by incorporating the key technologies, including the directional presplittingroof cutting, constant resistance and large deformation （CRLD） bolt/anchor supporting systemwith negative Poisson＇s ratio （NPR） effect material, and remote real-time monitoring technology. TheCCBT and 110 mining method will provide the theoretical and technical basis for the development ofmining industry in China.
基金financially supported by the State Key Research Development Program of China(Grant No.2016YFC0600701)the National Natural Science Foundation of China(Grant No.51674170)
文摘In this study, the spatial distributions of stress and fracture fields for three typical underground coal mining layouts, Le, non-pillar mining （NM）, top-coal caving mining （TCM） and protective coal-seam mining （PCM）, are modeled using discrete element software UDEC, The numerical results show that different mining layouts can lead to different mining-induced stress fields, resulting in diverse fracture fields, For the PCM, the mining influenced area in front of the mining faces is the largest, and the stress concentration factor in front of the mining faces is the lowest, The spatial shapes of the mining-induced fracture fields under NM, TCM and PCM differ, and they are characterized by trapezoidal, triangular and tower shapes, respectively, The fractal dimensions of mining-induced fractures of the three mining layouts decrease in the order of PCM, TCM and NM, It is also shown that the PCM can result in a better gas control effect in coal mines with high outburst potential, The numerical results are expected to provide a basis for understanding of mining-induced gas seepage fields and provide a reference for high- efficiency coal mining,
文摘The rehabilitation of coal mines is an up-to-date issue, heavily discussed not only by the industry but also by scientists and the adjacent communities since open cut coal mining has extensive impacts affecting all parties. Whereas, before and during the mining process not only nature but sometimes also the population of a whole region has to give way to a mine, the consequences of the operations remain long after mining closure. Typically a once intact and functioning ecosystem is replaced with a destroyed wideness evocative of a moon landscape. Fortunately, the awareness of the need to rehabilitate the destroyed nature is increasing steadily and all involved parties are making efforts to improve the situation, even if it is a major challenge to bring all parties to a consensus. Therefore, a legislative basis is required and the know-how of optimal rehabilitation solutions must be developed. In this article the rehabilitation of mined land in Australia and Germany are compared. More precisely the article will present an overview of the legislative regulations, certain applied methods of rehabilitation work and the problems that are likely to occur in respect to the influence of mining on nature.
基金supported by the National Natural Science Foun dation of China(52374170 and 51974313)the National Key Research and Development Plan Project(2022YFF1303300).
文摘1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrestrial ecosystems.
基金financially supported by the National Key Research and Development Program of China(Grant No.2023YFC2811600)the National Natural Science Foundation of China(Grant Nos.52301349 and 52088102)+1 种基金the Qingdao Post-Doctorate Science Fund(No.QDBSH20220202070)the Major Scientific and Technological Innovation Project of Shandong Province(Grant No.2019JZZY010820).
文摘A deep-sea mining riser is a crucial component of the system used to lift seafloor mineral resources to the vessel.It is prone to damage and failure because of harsh environmental conditions and internal fluid erosion.Furthermore,damage can impact the response characteristics of the riser,but varying environmental loadings easily mask it.Thus,distin-guishing between riser damage and environmental effects poses a considerable challenge.To address this issue,a cantilevered model is created for a deep-sea mining riser via the concentrated mass method,and a time-domain analytical strategy is developed.The vortex-induced vibration(VIV)response characteristics of the riser are initially examined,considering various damage conditions and flow velocities.The study results revealed four primary observations:(a)effective tension can serve as a reliable indicator for identifying damage at lower velocities;(b)there are noticeable differences in displacement between the healthy and damaged risers in the in-line direction rather than the cross-flow direction;(c)frequency characteristics can more effectively distinguish the damage conditions at high flow velocities,with the mean square frequency and frequency variance being more effective than the centroid frequency and root variance frequency;(d)displacement differences are more sensitive to damage occurring near the top and bottom of the riser,while both velocity variations and structural damage can influence displacements,especially in regions between modal nodes.The vibrational behavior and damage indicators are clarified for structural health monitoring of deep-sea mining risers during lifting operations.
基金Under the National Key R&D Program Key Project(No.2021YFC3201201)National Natural Science Foundation of China(No.52360032)+2 种基金Basic Scientific Research Business Fee Project of Colleges And Universities Directly Under the Inner Mongolia Autonomous Region(No.JBYYWF2022001)Development Plan of Innovation Team of Colleges And Universities in Inner Mongolia Autonomous Region(No.NMGIRT2313)the Innovation Team of‘Grassland Talents’。
文摘Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base in China with significant strategic importance,has undergone intensive coal mining activities that have substantially disrupted regional groundwater circulation.This study integrated data from the Gravity Recovery and Climate Experiment Satellite(GRACE)and Famine Early Warning Systems Network(FEWS NET)Land Data Assimilation System(FLDAS)models,combined with weighted downscaling methodology and water balance principles,to reconstruct high-resolution(0.01°)terrestrial water storage(TWS)and GWS changes in the Ordos Mining Region,China from April 2002 to December 2021.The accuracy of GWS variations were validated through pumping test measurements.Subsequently,Geodetector analysis was implemented to quantify the contributions of natural and anthropogenic factors to groundwater storage dynamics.Key findings include:1)TWS in the study area showed a fluctuating but overall decreasing trend,with a total reduction of 8901.11 mm during study period.The most significant annual decrease occurred in 2021,reaching 1696.77 mm.2)GWS exhibited an accelerated decline,with an average annual change rate of 44.35 mm/yr,totaling a decrease of 887.05 mm.The lowest annual groundwater storage level was recorded in 2020,reaching 185.69 mm.3)Precipitation(PRE)contributed the most to GWS variation(q=0.52),followed by coal mining water consumption(MWS)(q=0.41).The interaction between PRE and MWS exhibited a nonlinear enhancement effect on GWS changes(0.54).The synergistic effect of natural hydrological factors has a great influence on the change of GWS,but coal mining water consumption will continue to reduce GWS.These findings provide critical references for the management and regulation of groundwater resource in mining regions.
文摘Copper smelting is the main source of arsenic pollution in the environment,and China is the largest country for copper smelting.Taking 2022 as an example,this study analyzes the distribution and fate of arsenic across the copper mining,beneficiation,and smelting processes using a life-cycle approach,providing important insights for arsenic pollution prevention and the resource utilization of arsenic-bearing solid waste.The results show that the amount of As in waste rock,tailing and concentrate are 53483 t,86632 t,76162 t,respectively.After smelting treatment,the amount of arsenic in different types of solid waste,wastewater,waste gas and products are 76128 t,1 t,31 t and 2 t,respectively,and the proportion in arsenic sulfide slag is the highest(55%).The amount of emission to the environment is 32 t,accounting for only 0.04%of total amount.In the future,key considerations are to improve the resource utilization rate of arsenic-containing solid waste(tailing,smelting slag),especially arsenic sulfide slag,and to digest its environmental risk.
基金Project(10872218) supported by the National Natural Science Foundation of ChinaProject(2010CB732004) supported by the National Key Basic Research Program of China+1 种基金Project(20090461022) supported by the National Postdoctoral Foundation of ChinaProject (11MX21) supported by the Students' Innovation Project Aubsidize Award of Arcelor Mittal
文摘Xinli district of Sanshandao Gold Mine is the first subsea metal mine in China.To achieve 6 kt/d production capacity under the premise of safe mining,high-intensity mining might destroy the in-situ stress filed and the stability of rockmass.According to sampling and testing of ore-rock and backfill and in-situ stress field measurement,safety factor method calculation model based on stress-strain strength reduction at arbitrary points and Mohr-Coulomb yield criterion was established and limit displacement subsidence values under the safety factor of different limit stoping steps were calculated.The results from three years in-situ mining and strata movement monitoring using multi-point displacements meter showed that the lower settlement frame stope hierarchical level filling mining method,mining sequence are reasonable and rockmass stability evaluation using safety factor method,in-situ real-time monitoring can provide the technical foundation for the safety of seabed mining.
基金supported by Technology Innovation Fund of China Coal Research Institute(2022CX-I-04)Science and Technology Innovation Venture Capital Project of China Coal Technology Engineering Group(2020-2-TD-CXY005)。
文摘Enhancing the mining speed of a working face has become the primary approach to achieve high production and efficiency in coal mines,thereby further improving the production capacity.However,the problem of rock bursts resulting from this approach has become increasingly serious.Therefore,to implement coal mine safety and efficient extraction,the impact of deformation pressure caused by different mining speeds should be considered,and a reasonable mining speed of the working face should be determined.The influence of mining speed on overlying rock breaking in the stope is analyzed by establishing a key layer block rotation and subsidence model.Results show that with the increasing mining speed,the compression amount of gangue in the goaf decreases,and the rotation and subsidence amount of rock block B above goaf decreases,forcing the rotation and subsidence amount of rock block A above roadway to increase.Consequently,the contact mode between rock block A and rock block B changes from line contact to point contact,and the horizontal thrust and shear force between blocks increase.The increase in rotation and subsidence of rock block A intensifies the compression degree of coal and rock mass below the key layer,thereby increasing the stress concentration degree of coal and rock mass as well as the total energy accumulation.In addition,due to the insufficient compression of gangue in the goaf,the bending and subsidence space of the far-field key layer are limited,the length of the suspended roof increases,and the influence range of mining stress and the energy accumulation range expand.Numerical test results and underground microseismic monitoring results verify the correlation between mining speed and stope energy,and high-energy events generally appear 1-2 d after the change in mining speed.On this basis,the statistical principle confirms that the maximum mining speed of the working face at 6 m/d is reasonable.
基金supported by the Outstanding Youth Foundation of Heilongjiang Province(No.YQ2021H009).
文摘In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids.Initially,potential endophytic fungi were identified through genome mining.Subsequently,fine fractions containing various fernane-type triterpenoids were selected using HSQC data collection and SMART prediction.These triterpenoids were then obtained through targeted isolation and identification.Finally,their antifungal activity was evaluated.As a result,three fernane-type triterpenoids,including two novel compounds,along with two new sesquiterpenes and four known compounds were isolated from one potential strain,Diaporthe discoidispora.Their structures were elucidated through analysis of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(NMR)spectroscopic data.The absolute configurations were determined using single-crystal X-ray diffraction analysis and electron capture detector(ECD)analysis.Compound 3 exhibited moderate antifungal activity against Candida albicans CMCC 98001 and Aspergillus niger.
