[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, includ...[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, including Chelex-100 method, PCR buffer method, organic phenol-chloroform method and centrifugal col- umn type kit method. The extracted DNA was analyzed by using PCR amplification and electrophoresis to compare these four DNA extraction methods. [Result] Accord- ing to the results of spectrophotometer detection and gel electrophoresis, nucleic acid extracted by Chetex-100 method had proteins and other impurities; nucleic acid ex- tracted by PCR buffer method was low in concentration; however, DNA extracted by organic phenol-chloroform method and centrifugal column type kit was high in con- centration with no impurity band. [Conclusion] This study had laid the strong founda- tion of scientific theory to further explore the efficient and simple method for extracting DNA from Wusuli raccoon dog hair follicle.展开更多
目的研究吸附性载体上微量血痕的DNA提取及其检验。方法用Chelex-100法、QIAamp MiniKit、及QIAamp Mini Kit改良法提取吸附性载体上微量血痕中的DNA,进行PCR扩增及STR检验。结果采用Chelex-100法及QIAamp Mini Kit的分型成功率很低;采...目的研究吸附性载体上微量血痕的DNA提取及其检验。方法用Chelex-100法、QIAamp MiniKit、及QIAamp Mini Kit改良法提取吸附性载体上微量血痕中的DNA,进行PCR扩增及STR检验。结果采用Chelex-100法及QIAamp Mini Kit的分型成功率很低;采用QIAamp Mini Kit的改良法能较好的得到分型图谱。结论采用QIAamp Mini Kit的改良法能较好的提取吸附性载体上微量血痕的模板DNA。展开更多
基金Supported by National Natural Science Foundation of China (31072018)~~
文摘[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, including Chelex-100 method, PCR buffer method, organic phenol-chloroform method and centrifugal col- umn type kit method. The extracted DNA was analyzed by using PCR amplification and electrophoresis to compare these four DNA extraction methods. [Result] Accord- ing to the results of spectrophotometer detection and gel electrophoresis, nucleic acid extracted by Chetex-100 method had proteins and other impurities; nucleic acid ex- tracted by PCR buffer method was low in concentration; however, DNA extracted by organic phenol-chloroform method and centrifugal column type kit was high in con- centration with no impurity band. [Conclusion] This study had laid the strong founda- tion of scientific theory to further explore the efficient and simple method for extracting DNA from Wusuli raccoon dog hair follicle.
文摘目的研究吸附性载体上微量血痕的DNA提取及其检验。方法用Chelex-100法、QIAamp MiniKit、及QIAamp Mini Kit改良法提取吸附性载体上微量血痕中的DNA,进行PCR扩增及STR检验。结果采用Chelex-100法及QIAamp Mini Kit的分型成功率很低;采用QIAamp Mini Kit的改良法能较好的得到分型图谱。结论采用QIAamp Mini Kit的改良法能较好的提取吸附性载体上微量血痕的模板DNA。
