BACKGROUND Necrotizing enterocolitis(NEC)remains a prominent gastrointestinal emergency among infants,particularly term infants with congenital heart defects(CHD)being at high risk.The molecular processes that contrib...BACKGROUND Necrotizing enterocolitis(NEC)remains a prominent gastrointestinal emergency among infants,particularly term infants with congenital heart defects(CHD)being at high risk.The molecular processes that contribute to NEC have yet to be completely understood.The high mortality rates necessitate an active search for noninvasive biomarkers that can aid in the preclinical diagnosis and prognosis of NEC.MicroRNAs(miRs),which are involved in many biological processes in both health and disease,have been discovered to play an important role in regulating inflammation and immune responses via various signaling pathways.AIM To determine the plasma levels of miR-155,miR-221,miR-223,miR-320a,miR-451a as potential NEC biomarkers in term newborns with CHD.METHODS This prospective cohort study included twenty-tree term newborns with CHD who underwent cardiac surgery on the median day of life(DOL)=7.Nine of them developed NEC(Bell’s stage IIA and IIIA)within 1 week of cardiac surgery(NEC newborns).Blood samples were collected before(median DOL=5)and following(median DOL=13)cardiac surgery.Levels of plasma miR-155-5p,miR-221-3p,miR-223-3p,miR-320a-3p,and miR-451a were determined using real-time polymerase chain reaction.The functional analysis was executed using the DIANA-miRPath v4.0.RESULTS Preoperatively,NEC newborns had significantly lower plasma levels of miR-155(2.70-fold,P=0.020),miR-223(2.42-fold,P=0.030),and miR-320a(3.62-fold,P=0.006)than newborns without NEC.Postoperatively,miR-451a levels differed significantly between the newborn groups,showing a 4.70-fold decrease(P=0.014)in expression when clinical NEC symptoms appeared.According to receiver operating characteristic analysis,miR-320a was found to be the most effective predictive biomarker for NEC[area under the curve(AUC)=0.835,63%sensitivity,100%specificity],while miR-451a was identified as a NEC biomarker(AUC=0.835,85.7%sensitivity,76.9%specificity).Preoperatively,miR-155-5p,miR-223-3p,and miR-320a-3p were differentially expressed and targeted the forkhead box O and Hippo pathways(P<0.01).CONCLUSION Our study demonstrates,for the first time,that plasma miR-320a-3p levels can be used as a preclinical biomarker for NEC in term newborns with CHD.展开更多
Objective: This study was aimed to explore the expression of microRNA-32(miR-32) in multiple myeloma(MM), and study its association with β2-microglobulin and staging of MM by Durie-Salmon classification. Methods: The...Objective: This study was aimed to explore the expression of microRNA-32(miR-32) in multiple myeloma(MM), and study its association with β2-microglobulin and staging of MM by Durie-Salmon classification. Methods: The expression level of miR-32 in bone marrow mono-nuclear cells of MM were examined by real-time polymerase chain reaction(real-time PCR), and the correlations between the expression level of miR-32 and related clinic pathologic features β2-microglobulin, staging of MM by Durie-Salmon classification were further analyzed. Results: The expression of miR-32 in MM patients was obviously higher than that in normal control(P < 0.05). The expression of miR-32 in relapsed/ refractory MM patients was obviously higher than that in newly diagnosed MM patients. The expression of miR-32 in MM patients decreased after chemical therapy than that before treatment, especially in effective group(P < 0.05). There was no statistically significant change in ineffective/progress group after chemical therapy(P > 0.05).The expression of miR-32 was associated to staging of MM and β2-microglobulin level. Conclusion: Expression level of miR-32 in MM patients is significantly higher than that in normal bone marrow, these data indicated that miR-32 may play an important role in the development of MM. High-regulated expression of miR-32 was associated with β2-microglobulin and staging of MM by Durie-Salmon classification.展开更多
目的:探究microRNA-324-5p(miR-324-5p)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达与病人预后危险因素。方法:通过TCGA(The Cancer Genome Atlas)数据库获得的肿瘤组织和正常肝组织miRNA表达谱数据与病人临床信息,分析miR-3...目的:探究microRNA-324-5p(miR-324-5p)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达与病人预后危险因素。方法:通过TCGA(The Cancer Genome Atlas)数据库获得的肿瘤组织和正常肝组织miRNA表达谱数据与病人临床信息,分析miR-324-5p表达差异与病理的关系。通过绘制生存曲线分析miR-324-5p表达与病人预后的相关性。单因素和多因素Cox回归模型分析HCC相关危险因素。结果:miR-324-5p在HCC表达高于正常组织(P<0.001),且与不良预后相关。多因素Cox分析表明miR-324-5p可作为HCC预后不良的独立危险因素(P<0.05)。结论:miR-324-5p在HCC组织过表达,是HCC病人总生存期缩短的独立危险因素,为HCC预后判断的潜在生物学标志物。展开更多
基金Supported by The Russian Science Foundation,No.19-75-20076.
文摘BACKGROUND Necrotizing enterocolitis(NEC)remains a prominent gastrointestinal emergency among infants,particularly term infants with congenital heart defects(CHD)being at high risk.The molecular processes that contribute to NEC have yet to be completely understood.The high mortality rates necessitate an active search for noninvasive biomarkers that can aid in the preclinical diagnosis and prognosis of NEC.MicroRNAs(miRs),which are involved in many biological processes in both health and disease,have been discovered to play an important role in regulating inflammation and immune responses via various signaling pathways.AIM To determine the plasma levels of miR-155,miR-221,miR-223,miR-320a,miR-451a as potential NEC biomarkers in term newborns with CHD.METHODS This prospective cohort study included twenty-tree term newborns with CHD who underwent cardiac surgery on the median day of life(DOL)=7.Nine of them developed NEC(Bell’s stage IIA and IIIA)within 1 week of cardiac surgery(NEC newborns).Blood samples were collected before(median DOL=5)and following(median DOL=13)cardiac surgery.Levels of plasma miR-155-5p,miR-221-3p,miR-223-3p,miR-320a-3p,and miR-451a were determined using real-time polymerase chain reaction.The functional analysis was executed using the DIANA-miRPath v4.0.RESULTS Preoperatively,NEC newborns had significantly lower plasma levels of miR-155(2.70-fold,P=0.020),miR-223(2.42-fold,P=0.030),and miR-320a(3.62-fold,P=0.006)than newborns without NEC.Postoperatively,miR-451a levels differed significantly between the newborn groups,showing a 4.70-fold decrease(P=0.014)in expression when clinical NEC symptoms appeared.According to receiver operating characteristic analysis,miR-320a was found to be the most effective predictive biomarker for NEC[area under the curve(AUC)=0.835,63%sensitivity,100%specificity],while miR-451a was identified as a NEC biomarker(AUC=0.835,85.7%sensitivity,76.9%specificity).Preoperatively,miR-155-5p,miR-223-3p,and miR-320a-3p were differentially expressed and targeted the forkhead box O and Hippo pathways(P<0.01).CONCLUSION Our study demonstrates,for the first time,that plasma miR-320a-3p levels can be used as a preclinical biomarker for NEC in term newborns with CHD.
基金Supported by grants from the Qingdao Public Sphere Support Program of Qingdao Municipal Science and Technology Commission(No.2010KZJ-9)Qingdao Public Health Bureau(No.2012-WSZD042)
文摘Objective: This study was aimed to explore the expression of microRNA-32(miR-32) in multiple myeloma(MM), and study its association with β2-microglobulin and staging of MM by Durie-Salmon classification. Methods: The expression level of miR-32 in bone marrow mono-nuclear cells of MM were examined by real-time polymerase chain reaction(real-time PCR), and the correlations between the expression level of miR-32 and related clinic pathologic features β2-microglobulin, staging of MM by Durie-Salmon classification were further analyzed. Results: The expression of miR-32 in MM patients was obviously higher than that in normal control(P < 0.05). The expression of miR-32 in relapsed/ refractory MM patients was obviously higher than that in newly diagnosed MM patients. The expression of miR-32 in MM patients decreased after chemical therapy than that before treatment, especially in effective group(P < 0.05). There was no statistically significant change in ineffective/progress group after chemical therapy(P > 0.05).The expression of miR-32 was associated to staging of MM and β2-microglobulin level. Conclusion: Expression level of miR-32 in MM patients is significantly higher than that in normal bone marrow, these data indicated that miR-32 may play an important role in the development of MM. High-regulated expression of miR-32 was associated with β2-microglobulin and staging of MM by Durie-Salmon classification.
文摘目的:探究microRNA-324-5p(miR-324-5p)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达与病人预后危险因素。方法:通过TCGA(The Cancer Genome Atlas)数据库获得的肿瘤组织和正常肝组织miRNA表达谱数据与病人临床信息,分析miR-324-5p表达差异与病理的关系。通过绘制生存曲线分析miR-324-5p表达与病人预后的相关性。单因素和多因素Cox回归模型分析HCC相关危险因素。结果:miR-324-5p在HCC表达高于正常组织(P<0.001),且与不良预后相关。多因素Cox分析表明miR-324-5p可作为HCC预后不良的独立危险因素(P<0.05)。结论:miR-324-5p在HCC组织过表达,是HCC病人总生存期缩短的独立危险因素,为HCC预后判断的潜在生物学标志物。
