This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis comp...This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis complex(TSC)combined with primary lymphedema(PLE).Additionally,it retrospectively analyzes the data of 16 previously reported cases of children with TSC combined with PLE to summarize the epidemiology,genetic diagnosis,and current main treatments of these patients.The report also speculates on the pathological and physiological mechanisms underlying TSC combined with PLE.TSC combined with PLE is rare;therefore,the report provides a theoretical basis for understanding the pathophysiological mechanisms and treatment options for patients with TSC and PLE.Comprehensive clinical management of TSC is essential due to the diverse and multiorgan nature of its manifestations,often requiring a multidisciplinary approach for newly diagnosed cases.展开更多
The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for lit...The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.展开更多
Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breedin...Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.展开更多
Objective Our previous studies established that microRNA(miR)-451 from human umbilical cord mesenchymal stem cell-derived exosomes(hUC-MSC-Exos)alleviates acute lung injury(ALI).This study aims to elucidate the mechan...Objective Our previous studies established that microRNA(miR)-451 from human umbilical cord mesenchymal stem cell-derived exosomes(hUC-MSC-Exos)alleviates acute lung injury(ALI).This study aims to elucidate the mechanisms by which miR-451 in hUC-MSC-Exos reduces ALI by modulating macrophage autophagy.Methods Exosomes were isolated from hUC-MSCs.Severe burn-induced ALI rat models were treated with hUC-MSC-Exos carrying the miR-451 inhibitor.Hematoxylin-eosin staining evaluated inflammatory injury.Enzyme-linked immunosorbnent assay measured lipopolysaccharide(LPS),tumor necrosis factor-α,and interleukin-1βlevels.qRT-PCR detected miR-451 and tuberous sclerosis complex 1(TSC1)expressions.The regulatory role of miR-451 on TSC1 was determined using a dual-luciferase reporter system.Western blotting determined TSC1 and proteins related to the mammalian target of rapamycin(mTOR)pathway and autophagy.Immunofluorescence analysis was conducted to examine exosomes phagocytosis in alveolar macrophages and autophagy level.Results hUC-MSC-Exos with miR-451 inhibitor reduced burn-induced ALI and promoted macrophage autophagy.MiR-451 could be transferred from hUC-MSCs to alveolar macrophages via exosomes and directly targeted TSC1.Inhibiting miR-451 in hUC-MSC-Exos elevated TSC1 expression and inactivated the mTOR pathway in alveolar macrophages.Silencing TSC1 activated mTOR signaling and inhibited autophagy,while TSC1 knockdown reversed the autophagy from the miR-451 inhibitor-induced.Conclusion miR-451 from hUC-MSC exosomes improves ALI by suppressing alveolar macrophage autophagy through modulation of the TSC1/mTOR pathway,providing a potential therapeutic strategy for ALI.展开更多
BACKGROUND Tuberous sclerosis complex(TSC)and primary lymphedema(PLE)are both rare diseases,and it is even rarer for both to occur in the same patient.In this work,we have provided a detailed description of a patient&...BACKGROUND Tuberous sclerosis complex(TSC)and primary lymphedema(PLE)are both rare diseases,and it is even rarer for both to occur in the same patient.In this work,we have provided a detailed description of a patient's clinical presentation,imaging findings,and treatment.And a retrospective analysis was conducted on 14 published relevant case reports.CASE SUMMARY A 16-year-old male came to our hospital for treatment due to right lower limb swelling.This swelling is already present from birth.The patient’s memory had been progressively declining.Seizures had occurred 1 year prior at an unknown frequency.The patient was diagnosed with TSC combined with PLE through multimodal imaging examination:Computed tomography,magnetic resonance imaging,and lymphoscintigraphy.The patient underwent liposuction.The swelling of the patient's right lower limb significantly improved after surgery.Epilepsy did not occur.after taking antiepileptic drugs and sirolimus.CONCLUSION TSC with PLE is a rare and systemic disease.Imaging can detect lesions of this disease,which are important for diagnosis and treatment.展开更多
This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta wit...This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta with varying levels of incorporation ranging from 10 to 50% cereals such as millet, fonio, and rice, tubers such as manioc and sweet potato, and legumes such as cowpea. The results showed that the incorporation of local products considerably improved the nutrient content of penne without being rejected by consumers. Sensory analysis showed that the best results were obtained with mixtures of sweet potato, cowpea, and wheat, as well as with rice, manioc, cowpea, and sample wheat. These results are of great importance to manufacturers in a world where developing nutritious and attractive food products is a crucial challenge.展开更多
The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam culti...The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam cultivars, including five from Dioscorea alata (cDa083, cDa053, cDa115, cDa150 and cDa266) and five from Dioscorea cayenensis-rotundata (cDr015, cDr027, cDr150, cDr206 and cDr148) with distinct agro-morphological characteristics, were used as material. A completely randomized block design with 3 replications was used. In each block, all cultivars and seed types were represented. When put into cultivation, the tubers produced by the vitroplants all germinated and gave 100% healthy plants, compared with 86% to 100% healthy plants for the seed tubers produced by the farmers. Yields per hectare ranged from 10 to 25 tonnes for seed tubers produced by in vitro plants, and from 4 to 9 tons for seed tubers produced by farmers. Seed tubers from vitroplants can therefore be recommended to farmers as a solution to the problem of seed tuber unavailability.展开更多
The tropical roots and tuber crops (R and T) consist of cassava, sweet potato, yams, elephant foot yam, taro, tannia and a couple of minor tuber crops. Tropical tuber crops are the third most important food crops afte...The tropical roots and tuber crops (R and T) consist of cassava, sweet potato, yams, elephant foot yam, taro, tannia and a couple of minor tuber crops. Tropical tuber crops are the third most important food crops after cereals and pulses. These crops play a crucial role in providing food and nutritional security to the rural masses in Africa, Latin America and parts of Asia. Cassava and sweet potato rank among the top 10 food crops produced in developing countries and contribute to about 6% of world’s dietary calories. In India, tropical tubers are grown mostly in states like Odisha, West Bengal, Andhra Pradesh, Kerala, Tamil Nadu, Bihar and North East covering 4.5% of the total area under vegetables with 5.7% of the total vegetable production. Low income farmers and most undernourished households in India depend on R and T crops. They value these crops for their high calorie and stable yields under conditions in which other crops may fail. Genetic diversity of major tropical tuber crops provides options for its diverse usage in “multicuisine”, “nutrition”, “feed”, health care” and renewable resources of processing firms. Water productivity is also higher, for example, to produce 1 kg of tubers, sweet potatoes require water (383 l), taro (606 l), yam (343 l), as compared to rice (1673 l), maize (1222 l), wheat (1827 l), etc. Tuber crops are one of most efficient producers of dry matter and edible energy. These crops have potential to reverse soil degradation. Cassava is already recognized as drought tolerant followed by yam. Tubers of taro, elephant foot yam and yams can be stored under zero energy conditions for 6 - 7 months unlike temperate potatoes in cold storage. The natural climatic resilience as well as inherent nutrition attributes, has been explored further. Such studies made these crops more robust to changing climate and as the source of nutrient enriched food bowls. Sweet potato variety Bhu Krishna is the first purple flesh variety in India containing (90 mg/100 g anthocyanin) coupled with starch 22% - 25%. Similarly, the orange flesh Bhu Sona containing beta carotene (14 mg/100 g) coupled with high starch 22% - 24% is the first variety having both high beta carotene and high starch. These varieties are tolerant to salinity and Bhu Krishna is resistant to weevil. Likewise, developed improved taro varieties resistant to biotic blight stress, tolerant to salinity and are enriched with micro nutrients. These improved varieties are used to breed the superior types further. All these climate resilient, nutritionally enriched varieties will have a greater impact on supplementing rainbow food for all. These can address the issues like “food insecurity”, “malnutrition”, diet related issue of mass consumers across the world. The analysis of innovations on enhancing adaptability, nutritional quality with a management matrix would help in strengthening future programme.展开更多
目的系统评估不同肉色甘薯种质资源中多酚类物质的含量差异,揭示总酚与总黄酮的相关性。方法以84份甘薯种质为研究材料,采用福林酚比色法和三氯化铝比色法,分别测定块根中总酚和总黄酮含量。通过单因素方差分析,比较不同肉色材料间的差...目的系统评估不同肉色甘薯种质资源中多酚类物质的含量差异,揭示总酚与总黄酮的相关性。方法以84份甘薯种质为研究材料,采用福林酚比色法和三氯化铝比色法,分别测定块根中总酚和总黄酮含量。通过单因素方差分析,比较不同肉色材料间的差异,运用Pearson相关分析研究总酚与总黄酮含量间的关系,并采用K-均值聚类法对种质资源进行分类。结果甘薯块根总酚含量变幅为0.25~2.08 g GAE/100 g DW,紫肉品种[(1.57±0.34)g GAE/100 g DW]显著高于其他肉色品种(P<0.05);总黄酮含量范围为0.11~0.66 g RE/100 g DW,紫肉品种[(0.45±0.09)g RE/100 g DW]同样显著高于其他肉色品种(P<0.05);总酚与总黄酮含量呈极显著正相关(r=0.920,P<0.01);聚类分析将所有材料分为3类,其中Ⅰ类13份均为紫肉品种,具有最高的多酚类物质含量。结论在本研究收集的甘薯种质资源中,紫肉品种的总酚与总黄酮含量显著高于其他肉色类型,且二者表现出显著的协同积累趋势。因此,本研究所涉及的紫肉甘薯可作为多酚类物质的优良天然来源。聚类分析结果为甘薯的差异化利用提供参考,可选择高多酚类品种用于功能性食品开发,中低含量类群中的品种可用于鲜食或常规加工。展开更多
文摘This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis complex(TSC)combined with primary lymphedema(PLE).Additionally,it retrospectively analyzes the data of 16 previously reported cases of children with TSC combined with PLE to summarize the epidemiology,genetic diagnosis,and current main treatments of these patients.The report also speculates on the pathological and physiological mechanisms underlying TSC combined with PLE.TSC combined with PLE is rare;therefore,the report provides a theoretical basis for understanding the pathophysiological mechanisms and treatment options for patients with TSC and PLE.Comprehensive clinical management of TSC is essential due to the diverse and multiorgan nature of its manifestations,often requiring a multidisciplinary approach for newly diagnosed cases.
文摘The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.
基金funded by the National Natural Science Foundation of China(Grant No.31801421)the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAAS-ASTIPIVFCAAS).
文摘Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.
基金supported by the tenth batch of"3221"industrial innovation and scientific research projects in Bengbu City(beng talent[2020]No.8)the 2021 Bengbu Medical College Science and Technology Project[Natural Science,Project Number:2021byzd217].
文摘Objective Our previous studies established that microRNA(miR)-451 from human umbilical cord mesenchymal stem cell-derived exosomes(hUC-MSC-Exos)alleviates acute lung injury(ALI).This study aims to elucidate the mechanisms by which miR-451 in hUC-MSC-Exos reduces ALI by modulating macrophage autophagy.Methods Exosomes were isolated from hUC-MSCs.Severe burn-induced ALI rat models were treated with hUC-MSC-Exos carrying the miR-451 inhibitor.Hematoxylin-eosin staining evaluated inflammatory injury.Enzyme-linked immunosorbnent assay measured lipopolysaccharide(LPS),tumor necrosis factor-α,and interleukin-1βlevels.qRT-PCR detected miR-451 and tuberous sclerosis complex 1(TSC1)expressions.The regulatory role of miR-451 on TSC1 was determined using a dual-luciferase reporter system.Western blotting determined TSC1 and proteins related to the mammalian target of rapamycin(mTOR)pathway and autophagy.Immunofluorescence analysis was conducted to examine exosomes phagocytosis in alveolar macrophages and autophagy level.Results hUC-MSC-Exos with miR-451 inhibitor reduced burn-induced ALI and promoted macrophage autophagy.MiR-451 could be transferred from hUC-MSCs to alveolar macrophages via exosomes and directly targeted TSC1.Inhibiting miR-451 in hUC-MSC-Exos elevated TSC1 expression and inactivated the mTOR pathway in alveolar macrophages.Silencing TSC1 activated mTOR signaling and inhibited autophagy,while TSC1 knockdown reversed the autophagy from the miR-451 inhibitor-induced.Conclusion miR-451 from hUC-MSC exosomes improves ALI by suppressing alveolar macrophage autophagy through modulation of the TSC1/mTOR pathway,providing a potential therapeutic strategy for ALI.
基金Supported by National Natural Science Foundation of China,No.61876216.
文摘BACKGROUND Tuberous sclerosis complex(TSC)and primary lymphedema(PLE)are both rare diseases,and it is even rarer for both to occur in the same patient.In this work,we have provided a detailed description of a patient's clinical presentation,imaging findings,and treatment.And a retrospective analysis was conducted on 14 published relevant case reports.CASE SUMMARY A 16-year-old male came to our hospital for treatment due to right lower limb swelling.This swelling is already present from birth.The patient’s memory had been progressively declining.Seizures had occurred 1 year prior at an unknown frequency.The patient was diagnosed with TSC combined with PLE through multimodal imaging examination:Computed tomography,magnetic resonance imaging,and lymphoscintigraphy.The patient underwent liposuction.The swelling of the patient's right lower limb significantly improved after surgery.Epilepsy did not occur.after taking antiepileptic drugs and sirolimus.CONCLUSION TSC with PLE is a rare and systemic disease.Imaging can detect lesions of this disease,which are important for diagnosis and treatment.
文摘This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta with varying levels of incorporation ranging from 10 to 50% cereals such as millet, fonio, and rice, tubers such as manioc and sweet potato, and legumes such as cowpea. The results showed that the incorporation of local products considerably improved the nutrient content of penne without being rejected by consumers. Sensory analysis showed that the best results were obtained with mixtures of sweet potato, cowpea, and wheat, as well as with rice, manioc, cowpea, and sample wheat. These results are of great importance to manufacturers in a world where developing nutritious and attractive food products is a crucial challenge.
文摘The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam cultivars, including five from Dioscorea alata (cDa083, cDa053, cDa115, cDa150 and cDa266) and five from Dioscorea cayenensis-rotundata (cDr015, cDr027, cDr150, cDr206 and cDr148) with distinct agro-morphological characteristics, were used as material. A completely randomized block design with 3 replications was used. In each block, all cultivars and seed types were represented. When put into cultivation, the tubers produced by the vitroplants all germinated and gave 100% healthy plants, compared with 86% to 100% healthy plants for the seed tubers produced by the farmers. Yields per hectare ranged from 10 to 25 tonnes for seed tubers produced by in vitro plants, and from 4 to 9 tons for seed tubers produced by farmers. Seed tubers from vitroplants can therefore be recommended to farmers as a solution to the problem of seed tuber unavailability.
文摘The tropical roots and tuber crops (R and T) consist of cassava, sweet potato, yams, elephant foot yam, taro, tannia and a couple of minor tuber crops. Tropical tuber crops are the third most important food crops after cereals and pulses. These crops play a crucial role in providing food and nutritional security to the rural masses in Africa, Latin America and parts of Asia. Cassava and sweet potato rank among the top 10 food crops produced in developing countries and contribute to about 6% of world’s dietary calories. In India, tropical tubers are grown mostly in states like Odisha, West Bengal, Andhra Pradesh, Kerala, Tamil Nadu, Bihar and North East covering 4.5% of the total area under vegetables with 5.7% of the total vegetable production. Low income farmers and most undernourished households in India depend on R and T crops. They value these crops for their high calorie and stable yields under conditions in which other crops may fail. Genetic diversity of major tropical tuber crops provides options for its diverse usage in “multicuisine”, “nutrition”, “feed”, health care” and renewable resources of processing firms. Water productivity is also higher, for example, to produce 1 kg of tubers, sweet potatoes require water (383 l), taro (606 l), yam (343 l), as compared to rice (1673 l), maize (1222 l), wheat (1827 l), etc. Tuber crops are one of most efficient producers of dry matter and edible energy. These crops have potential to reverse soil degradation. Cassava is already recognized as drought tolerant followed by yam. Tubers of taro, elephant foot yam and yams can be stored under zero energy conditions for 6 - 7 months unlike temperate potatoes in cold storage. The natural climatic resilience as well as inherent nutrition attributes, has been explored further. Such studies made these crops more robust to changing climate and as the source of nutrient enriched food bowls. Sweet potato variety Bhu Krishna is the first purple flesh variety in India containing (90 mg/100 g anthocyanin) coupled with starch 22% - 25%. Similarly, the orange flesh Bhu Sona containing beta carotene (14 mg/100 g) coupled with high starch 22% - 24% is the first variety having both high beta carotene and high starch. These varieties are tolerant to salinity and Bhu Krishna is resistant to weevil. Likewise, developed improved taro varieties resistant to biotic blight stress, tolerant to salinity and are enriched with micro nutrients. These improved varieties are used to breed the superior types further. All these climate resilient, nutritionally enriched varieties will have a greater impact on supplementing rainbow food for all. These can address the issues like “food insecurity”, “malnutrition”, diet related issue of mass consumers across the world. The analysis of innovations on enhancing adaptability, nutritional quality with a management matrix would help in strengthening future programme.
文摘目的系统评估不同肉色甘薯种质资源中多酚类物质的含量差异,揭示总酚与总黄酮的相关性。方法以84份甘薯种质为研究材料,采用福林酚比色法和三氯化铝比色法,分别测定块根中总酚和总黄酮含量。通过单因素方差分析,比较不同肉色材料间的差异,运用Pearson相关分析研究总酚与总黄酮含量间的关系,并采用K-均值聚类法对种质资源进行分类。结果甘薯块根总酚含量变幅为0.25~2.08 g GAE/100 g DW,紫肉品种[(1.57±0.34)g GAE/100 g DW]显著高于其他肉色品种(P<0.05);总黄酮含量范围为0.11~0.66 g RE/100 g DW,紫肉品种[(0.45±0.09)g RE/100 g DW]同样显著高于其他肉色品种(P<0.05);总酚与总黄酮含量呈极显著正相关(r=0.920,P<0.01);聚类分析将所有材料分为3类,其中Ⅰ类13份均为紫肉品种,具有最高的多酚类物质含量。结论在本研究收集的甘薯种质资源中,紫肉品种的总酚与总黄酮含量显著高于其他肉色类型,且二者表现出显著的协同积累趋势。因此,本研究所涉及的紫肉甘薯可作为多酚类物质的优良天然来源。聚类分析结果为甘薯的差异化利用提供参考,可选择高多酚类品种用于功能性食品开发,中低含量类群中的品种可用于鲜食或常规加工。
