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Efficient implementation of the k-√k L turbulence model with the discontinuous Galerkin method
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作者 Zhenhua Jiang Chao Yan +1 位作者 Jian Yu Yao Li 《Acta Mechanica Sinica》 2025年第4期70-81,共12页
We present the approaches to implementing the k-√k L turbulence model within the framework of the high-order discontinuous Galerkin(DG)method.We use the DG discretization to solve the full Reynolds-averaged Navier-St... We present the approaches to implementing the k-√k L turbulence model within the framework of the high-order discontinuous Galerkin(DG)method.We use the DG discretization to solve the full Reynolds-averaged Navier-Stokes equations.In order to enhance the robustness of approaches,some effective techniques are designed.The HWENO(Hermite weighted essentially non-oscillatory)limiting strategy is adopted for stabilizing the turbulence model variable k.Modifications have been made to the model equation itself by using the auxiliary variable that is always positive.The 2nd-order derivatives of velocities required in computing the von Karman length scale are evaluated in a way to maintain the compactness of DG methods.Numerical results demonstrate that the approaches have achieved the desirable accuracy for both steady and unsteady turbulent simulations. 展开更多
关键词 Discontinuous Galerkin method k-√k L turbulence model High-order scheme
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What is the Most Effective Method of Error Correction in L2 Writing? 被引量:2
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作者 顾红兵 《海外英语》 2011年第10X期37-39,共3页
The efficacy of error correction and various kinds of correction approaches is one of the key issues in second language writing faced by both teachers and researchers. The current paper reviews the definition of error... The efficacy of error correction and various kinds of correction approaches is one of the key issues in second language writing faced by both teachers and researchers. The current paper reviews the definition of error correction and examines the different views on whether error correction in L2 writing should be corrected. In particular, the paper discusses and analyses the three common correction methods: direct correction, peer feedback and indirect correction. Teachers are encouraged to weigh and analyze the advantages and disadvantages of these methods according to the current literature, employ the most beneficial error correction method in L2 writing, and adapt its suitability to their teaching context. 展开更多
关键词 ERROR CORRECTION L2 WRITING effective method
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SYNTHESIS OF ULTRAFINE PLZT POWDER BY A SIMPLE CO-PRECIPITATION METHOD 被引量:14
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作者 H. Ying1) , X. D. Sun1) , Q. B. Yang1) , J. B. Li2) , Y. M. Wang2) , Z. Zhang3) and J. You3)1) School of Materials and Metallurgy , Northeastern University , Shenyang 110006 , China2) Departm ent of Materials , School of Mechani 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 1999年第5期1180-1183,共4页
Starting from cheap inorganic salts , ultrafine P L Z T po w ders were synthesized by a co pre cipitation m ethod . The results show that the precursor precipitate obtained by the co precipita tion method transf... Starting from cheap inorganic salts , ultrafine P L Z T po w ders were synthesized by a co pre cipitation m ethod . The results show that the precursor precipitate obtained by the co precipita tion method transfor ms co m pletely to P L Z T phase by calcining at 420 ℃ for 4 hours , about300 ℃low er than the nor m al transform ation te m perature of P L Z T obtained by m ixed oxidemethod . The effect of residual N H4 N O3 in the precursor precipitate on transfor m ation te m perature of the P L Z Tpow der w as investigated . The residual N H4 N O3 in the precursor po w der can reduce further the P L Z Ttransfor m ation tem perature to about 300 ℃, but a sm alla mount of Zr O2 and Pb Ti O3 re m ained . By a proper control of the synthesizing route , a P L Z T po w der with a pri m ary particle size of 10 n m and an agglom eration size of 0 3 μm hasbeen produced . 展开更多
关键词 P L Z T ultra fine po w der co precipitation
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Fabrication of Multi-layered Composite Scaffolds by Bi-directional Electrospinning Method 被引量:2
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作者 WU Tong HUANG Chen +2 位作者 CHEN Jian-feng LIU Wei MO Xiu-mei 《Journal of Donghua University(English Edition)》 EI CAS 2014年第5期625-629,共5页
A multi-layered composite scaffolds consisting of poly ( L- ne) ( P (LLA-CL) ), collagen (COL) and chitosan (CS) were fabricated by a bi-directional electrospinnlng method. Synthetic P (LLA-CL) was used as... A multi-layered composite scaffolds consisting of poly ( L- ne) ( P (LLA-CL) ), collagen (COL) and chitosan (CS) were fabricated by a bi-directional electrospinnlng method. Synthetic P (LLA-CL) was used as the middle layer to enhance the strength, while natural COL/CS blending (9: 1, v/v) was used as the bioactive surfaces (inner and outer layers ) to improve the biocompatibility. Each three transitional layers were set between inner/outer layer and middle layer for delamination resistance. Scanning electron microscopy (SEM) was used to observe the fiber morphology. The Fourier transform infrared attenuated total reflectance spectroscopy (FTIR-ATR) spectra, X- ray diffraction (XRD) and thermogravimetry (TG) tests were used to analyze the physical properties of the scaffolds. The results showed that the modified clectrospinning method bad no negative effect on the components, crystal structure and thermostability of the scaffolds, but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials. Such method could be applied to the fabrication of composite scaffolds for vascular, skin. and nerve tissue engineering. 展开更多
关键词 ELECTROSPINNING poly ( L.lactide-co-ε-caprolactone )( P(LLA-CL) ) COLLAGEN CHITOSAN composite NANofIBERS
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Effects of Different Storage Methods on the Fruit Quality and Physiological Changes in Yihong Honey Peach 被引量:1
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作者 CHEN Wei-feng YANG Yu +1 位作者 LONG Li-ping DENG Wen 《Agricultural Science & Technology》 CAS 2022年第4期15-24,共10页
Taking Yihong,the main cultivar of mid-ripening honey peaches(Prunus persica)in Hunan,as material,this study explored the effects of different storage methods on the fruit quality and physiological changes in Yihong h... Taking Yihong,the main cultivar of mid-ripening honey peaches(Prunus persica)in Hunan,as material,this study explored the effects of different storage methods on the fruit quality and physiological changes in Yihong honey peach by the combination of polyethylene film packaging with low-temperature storage.The results showed that the weight loss rate of packaged storage at normal temperature(T1)was significantly lower than that of unpackaged storage at normal temperature(CK),but there was no significant difference in other indicators.In the middle of storage,the respiration rate and ethylene release of packaged storage at 0℃(T3)were extremely significantly lower or significantly lower than those of unpackaged storage at 0℃(T2),packaged storage at 0℃after cold adapting at 8℃for 5 d(T4),and packaged storage in a fluctuating temperature range of 0~3.5℃(T5).After storage,the structure of the middle lamella in the cell wall of T3 was compact and uniform,and the mitochondrial structure was complete,while the cell walls and mitochondria of T4 and T5 were severely deformed and disintegrated.The activities of antioxidant enzymes peroxidase(POD)and superoxide dismutase(SOD)of T3 was significantly higher than those of T4 and T5,and the content of malondialdehyde(MDA)and polyphenol oxidase(PPO)of T3 was also the lowest.Therefore,0°C packaged storage can effectively delay the senescence of Yihong honey peach and prolong its freshness period. 展开更多
关键词 Honey peach(Prunus persica L.Batsch) Yihong Packaging Storage methods Physiological index ULTRASTRUCTURE
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Superconvergence of nonconforming finite element penalty scheme for Stokes problem using L^2 projection method 被引量:3
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作者 石东洋 裴丽芳 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2013年第7期861-874,共14页
A modified penalty scheme is discussed for solving the Stokes problem with the Crouzeix-Raviart type nonconforming linear triangular finite element. By the L^2 projection method, the superconvergence results for the v... A modified penalty scheme is discussed for solving the Stokes problem with the Crouzeix-Raviart type nonconforming linear triangular finite element. By the L^2 projection method, the superconvergence results for the velocity and pressure are obtained with a penalty parameter larger than that of the classical penalty scheme. The numerical experiments are carried out to confirm the theoretical results. 展开更多
关键词 SUPERCONVERGENCE Crouzeix-Raviart type nonconforming finite element penalty scheme L^2 projection method
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Application for Superconvergence of Finite Element Approximations for the Elliptic Problem by Global and Local L<sup>2</sup>-Projection Methods 被引量:1
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作者 Rabeea H. Jari Lin Mu 《American Journal of Computational Mathematics》 2012年第4期249-257,共9页
Numerical experiments are given to verify the theoretical results for superconvergence of the elliptic problem by global and local L2-Projection methods.
关键词 FINITE Element methods SUPERCONVERGENCE L2-Projection ELLIPTIC Problem
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Investigation of the Tikhonov Regularization Method in Regional Gravity Field Modeling by Poisson Wavelets Radial Basis Functions 被引量:2
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作者 Yihao Wu Bo Zhong Zhicai Luo 《Journal of Earth Science》 SCIE CAS CSCD 2018年第6期1349-1358,共10页
The application of Tikhonov regularization method dealing with the ill-conditioned problems in the regional gravity field modeling by Poisson wavelets is studied. In particular, the choices of the regularization matri... The application of Tikhonov regularization method dealing with the ill-conditioned problems in the regional gravity field modeling by Poisson wavelets is studied. In particular, the choices of the regularization matrices as well as the approaches for estimating the regularization parameters are investigated in details. The numerical results show that the regularized solutions derived from the first-order regularization are better than the ones obtained from zero-order regularization. For cross validation, the optimal regularization parameters are estimated from L-curve, variance component estimation(VCE) and minimum standard deviation(MSTD) approach, respectively, and the results show that the derived regularization parameters from different methods are consistent with each other. Together with the firstorder Tikhonov regularization and VCE method, the optimal network of Poisson wavelets is derived, based on which the local gravimetric geoid is computed. The accuracy of the corresponding gravimetric geoid reaches 1.1 cm in Netherlands, which validates the reliability of using Tikhonov regularization method in tackling the ill-conditioned problem for regional gravity field modeling. 展开更多
关键词 regional gravity field modeling Poisson wavelets radial basis functions Tikhonov regularization method L-CURVE variance component estimation(VCE)
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Reconstruction of high order derivatives by new mollification methods
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作者 赵振宇 贺国强 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2008年第6期769-778,共10页
In this paper, the problem of reconstructing numerical derivatives from noisy data is considered. A new framework of mollification methods based on the L generalized solution regularization methods is proposed. A spec... In this paper, the problem of reconstructing numerical derivatives from noisy data is considered. A new framework of mollification methods based on the L generalized solution regularization methods is proposed. A specific algorithm for the first three derivatives is presented in the paper, in which a modification of TSVD, termed cTSVD is chosen as the regularization technique. Numerical examples given in the paper verify the theoretical results and show efficiency of the new method. 展开更多
关键词 ill-posed problem numerical differentiation mollification method L generalized solution cTSVD method
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Extraction Process of Green Pigment from Leaves of Arctium lappa L. by Ultrasound Assisted Method and Its Stability Study
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作者 Yongxia KE Chunlin LIU +3 位作者 Xuexue LI Xiaoxia CAI Jingxia WANG Yuan LIU 《Medicinal Plant》 CAS 2019年第1期43-45,共3页
[Objectives] The research aimed to extract green pigment from leaves of Arctium lappa L. and inspect its stability. [Methods] Ultrasound assisted method was used. [Results] Water bath temperature had little impact on ... [Objectives] The research aimed to extract green pigment from leaves of Arctium lappa L. and inspect its stability. [Methods] Ultrasound assisted method was used. [Results] Water bath temperature had little impact on green pigment, and Al^(3+), Cu^(2+), Mg^(2+) and Zn^(2+) in metal ions greatly affected green pigment, while Fe^(3+) had very little impact on the stability of green pigment. pH did not basically affect green pigment of A. lappa L. [Conclusions] The extraction rate and stability of green pigment from leaves of A. lappa L. were higher, and it was one of materials suitable for extracting green pigment. 展开更多
关键词 LEAVES of Arctium lappa L. Green PIGMENT ULTRASOUND ASSISTED method
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Processes of the displacement field change of the 2009 April 6 M_W6.3 L'Aquila earthquake using persistent scatterer and small baseline methods
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作者 Sanming Luo Liming Fu +3 位作者 Shuang Zhu Qinglong He Wenni Wan Bo Yang 《Earthquake Science》 2013年第5期293-299,共7页
Using a time series method that combines both the persistent scatterer and small baseline approaches, we analyzed 9 scenes Envisat ASAR data over the L'Aquila earthquake, and obtained a Shocke's displacement field a... Using a time series method that combines both the persistent scatterer and small baseline approaches, we analyzed 9 scenes Envisat ASAR data over the L'Aquila earthquake, and obtained a Shocke's displacement field and its evolution processes. The results show that: (1) Envisat ASAR clearly detected the whole processes of displacement field of the L'Aquila earthquake, and distinct variations at different stages of the displacement field. (2) Preseismic creep displacement → displacement mutation when faulting → constantly slowed down after the earthquake. (3) The area of the strongest deformation and ground rupture was a low-lying oval depression region to the southeast. Surface faulting within a zone of about 22 km× 14 km, with an orientation of 135°, occurred along the NW-striking and SW-dipping Paganica-S. Demetrio normal fault. (4) In analyzing an area of about 54 km x 59 km, bounded by north-south axis to the epicenter, the displacement field has significant characteristics of a watershed: westward of the epicenter shows uplift with maximum of 130 mm in line-of-sight (LOS), and east of the epicenter was a region with 220 mm of maximum subsidence in the LOS, concentrating on the rupture zone, the majority of which formed in the course of faulting and subsequence. 展开更多
关键词 PS method SB method Incorporatingprocessing Time series L'Aquila earthquake Displacement field
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Yield Stability of Maize Hybrids Evaluated in Maize Regional Trials in Southwestern China Using Nonparametric Methods
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作者 LIU Yong-jian DUAN Chuan +2 位作者 TIAN Meng-liang HU Er-liang HUANG Yu-bi 《Agricultural Sciences in China》 CAS CSCD 2010年第10期1413-1422,共10页
Analysis of multi-environment trials (METs) of crops for the evaluation and recommendation of varieties is an important issue in plant breeding research. Evaluating on the both stability of performance and high yiel... Analysis of multi-environment trials (METs) of crops for the evaluation and recommendation of varieties is an important issue in plant breeding research. Evaluating on the both stability of performance and high yield is essential in MET analyses. The objective of the present investigation was to compare 11 nonparametric stability statistics and apply nonparametric tests for genotype-by-environment interaction (GEI) to 14 maize (Zea mays L.) genotypes grown at 25 locations in southwestern China during 2005. Results of nonparametric tests of GEl and a combined ANOVA across locations showed that both crossover and noncrossover GEI, and genotypes varied highly significantly for yield. The results of principal component analysis, correlation analysis of nonparametric statistics, and yield indicated the nonparametric statistics grouped as four distinct classes that corresponded to different agronomic and biological concepts of stability. Furthermore, high values of TOP and low values of rank-sum were associated with high mean yield, but the other nonparametric statistics were not positively correlated with mean yield. Therefore, only rank-sum and TOP methods would be useful for simultaneously selection for high yield and stability. These two statistics recommended JY686 and HX168 as desirable and ND108, CM12, CN36, and NK6661 as undesirable genotypes. 展开更多
关键词 Zea mays L. genotype environment multi-environment trials nonparametric methods
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EFFECTS OF REACTION AND PROCESSING PARAMETERS ON ETHYLENE POLYMERIZATION USING DIFFERENT ZIEGLER-NATTA CATALYSTS:EMPLOYMENT OF TAGUCHI EXPERIMENTAL DESIGN AND RESPONSE SURFACE METHOD
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作者 Mohammad Najafi Vahid Haddadi-Asl 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2007年第2期153-162,共10页
Different Ziegler-Natta catalysts were employed to polymerize ethylene. To investigate the influences of reaction parameters, namely Al/Ti molar ratio, hydrogen and processing parameters, i.e. ethylene pressure and te... Different Ziegler-Natta catalysts were employed to polymerize ethylene. To investigate the influences of reaction parameters, namely Al/Ti molar ratio, hydrogen and processing parameters, i.e. ethylene pressure and temperature, a Taguchi experimental design was worked out. An L27 orthogonal array was chosen to take the above-mentioned parameters and relevant interactions into account. Response surface method was the tool used to analyze the experimental design results. Al/Ti, ethylene pressure and temperature were selected as experimental design factors, and catalyst activity and polymerization yield were the response parameters. Increasing pressure, due to an increment in monomer accessibility, and rising Al/Ti, because of higher reduction in the catalysts, cause an increase in both polymerization yield and catalyst activity. Nonetheless, a higher temperature, thanks to reducing ethylene solubility in the slurry medium and partially catalyst destruction, lead to a reduction in both response parameters. A synergistic effect was also observed between temperature and pressure. All catalyst activities will reduce in the presence of hydrogen. Molecular weight also shows a decline in the presence of hydrogen as a transfer agent. However, the polydispersity index remains approximately intact. Using SEM, various morphologies, owing to different catalyst morphologies, were seen for the polyethylene. 展开更多
关键词 L27 Taguchi experimental design Response surface method Polyethylene polymerization Ziegler-Natta catalysts.
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Electrochemical Method for Evaluating the Passivated State of Medical StainlessSteel 317L
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作者 Lin Zhang Baofeng Ding +4 位作者 Yinshun Wu X. Wang Bei Cao Baofen Qi Fengmei Ren(Material Science and Engineering School, University of Science and Technology Beijing, Beijing 100083, China)(Tianjin Medical Instrument Inspection Center, Tianjin 300191, China 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2000年第2期135-138,共4页
The electrochemical behavior of the medical Stainless steel 317L which is used as in vivo fixation materials has been investigated at different passivated states in several candidate-testing solutions. The potentiodyn... The electrochemical behavior of the medical Stainless steel 317L which is used as in vivo fixation materials has been investigated at different passivated states in several candidate-testing solutions. The potentiodynamic scanning polarizaion technique was employed to measure the polarizaion curves of 317L in 0.9%NaCl solution at 37℃. The results showed that the electrochemical behaviorin 0.9% NaCl solution at 37℃ and in 2% NaCl solotion at 30℃ can be better used to detect and evaluate passivated states and corrosionresistance of 317L. In addition,the pitting potential Eb can be used as a criterion and itS lower limit could be 0.85 V(SEC) for this system. 展开更多
关键词 stainless steel 317L PASSIVATION pitting potential electrochemica1 method
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Optimizing Average Electric Power During the Charging of Lithium-Ion Batteries Through the Taguchi Method
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作者 Mohd H.S.Alrashdan 《Transactions of Tianjin University》 EI CAS 2024年第2期152-166,共15页
In recent times, lithium-ion batteries have been widely used owing to their high energy density, extended cycle lifespan, and minimal self-discharge rate. The design of high-speed rechargeable lithium-ion batteries fa... In recent times, lithium-ion batteries have been widely used owing to their high energy density, extended cycle lifespan, and minimal self-discharge rate. The design of high-speed rechargeable lithium-ion batteries faces a significant challenge owing to the need to increase average electric power during charging. This challenge results from the direct influence of the power level on the rate of chemical reactions occurring in the battery electrodes. In this study, the Taguchi optimization method was used to enhance the average electric power during the charging process of lithium-ion batteries. The Taguchi technique is a statistical strategy that facilitates the systematic and efficient evaluation of numerous experimental variables. The proposed method involved varying seven input factors, including positive electrode thickness, positive electrode material, positive electrode active material volume fraction, negative electrode active material volume fraction, separator thickness, positive current collector thickness, and negative current collector thickness. Three levels were assigned to each control factor to identify the optimal conditions and maximize the average electric power during charging. Moreover, a variance assessment analysis was conducted to validate the results obtained from the Taguchi analysis. The results revealed that the Taguchi method was an eff ective approach for optimizing the average electric power during the charging of lithium-ion batteries. This indicates that the positive electrode material, followed by the separator thickness and the negative electrode active material volume fraction, was key factors significantly infl uencing the average electric power during the charging of lithium-ion batteries response. The identification of optimal conditions resulted in the improved performance of lithium-ion batteries, extending their potential in various applications. Particularly, lithium-ion batteries with average electric power of 16 W and 17 W during charging were designed and simulated in the range of 0-12000 s using COMSOL Multiphysics software. This study efficiently employs the Taguchi optimization technique to develop lithium-ion batteries capable of storing a predetermined average electric power during the charging phase. Therefore, this method enables the battery to achieve complete charging within a specific timeframe tailored to a specificapplication. The implementation of this method can save costs, time, and materials compared with other alternative methods, such as the trial-and-error approach. 展开更多
关键词 Lithium-ion batteries Average electric power during charging Taguchi method COMSOL Multiphysics software C rate L27 orthogonal array
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Chaotic Control of LüSystem via Three Methods
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作者 Mingjun Wang 《International Journal of Modern Nonlinear Theory and Application》 2014年第2期29-36,共8页
In the paper, several different methods are adopted to control Lü system. With direct feedback method, Lü system can be stabilized at one equilibrium point or a limit cycle surrounding its equilibrium. With ... In the paper, several different methods are adopted to control Lü system. With direct feedback method, Lü system can be stabilized at one equilibrium point or a limit cycle surrounding its equilibrium. With adaptive time-delayed feedback method, feedback coefficient and delay time can be adjusted adaptively to stabilize Lü system at its original unstable periodic orbit. With minimal energy method, Lü system can also be stabilized at fixed point or limit cycle. Numerical simulations show the effectiveness of our methods. 展开更多
关键词 SYSTEM DIRECT FEEDBACK ADAPTIVE Time-Delayed FEEDBACK MINIMAL ENERGY method
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A Literature Review of the Research Method in the Study of L2 Pragmatic Competence of Chinese Tertiary-Level Students
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作者 SUN Han 《Sino-US English Teaching》 2021年第12期377-382,共6页
This study combs through the frequently cited papers on L2 pragmatic competence of Chinese tertiary-level students from CNKI,and analyzes the research methods used in these studies.The findings are:Metapragmatic judge... This study combs through the frequently cited papers on L2 pragmatic competence of Chinese tertiary-level students from CNKI,and analyzes the research methods used in these studies.The findings are:Metapragmatic judgement item,discourse completion task,role play,and verbal reports are the most widely used instruments in the study of L2 pragmatic competence of Chinese tertiary-level students.Besides,other methods like natural comprehension and elicited conversation have received more attention in recent years. 展开更多
关键词 L2 pragmatics interlanguage pragmatics pragmatic competence research method
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Study on the Determination Method for the Content of Tannin in Sanguisorba officinalis L.
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作者 Siwei CHEN Qin SUN +2 位作者 Fang WANG Xin YU Dan ZHANG 《Medicinal Plant》 2017年第1期20-21,共2页
[Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. ... [Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. [Methods] With gallic acid as reference substance,the UV method was used to determine the content of tannin in Sanguisorba officinalis L.,and the methodological examination was done. [Results]With gallic acid as standard,when the concentration of tannin in Sanguisorba officinalis L. was 1. 02-10. 14 μg·m L^(-1),there was a good linear relationship with absorbance,the precision,reproducibility and recovery were in line with the requirements,and the absorbance value was stable within 40 min after coloration. [Conclusions] The method was simple,rapid and accurate in determining the content of tannin in Sanguisorba officinalis L. 展开更多
关键词 Sanguisorba ofFICINALIS L. TANNIN ABSORBANCE Gallic ACID UV method
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Rapid Determination of Seed Number and Thousand Kernel Weight of Asparagus officinalis L.Using Image Processing Method
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作者 Huimin GAO Yu TIAN +1 位作者 Zhiqiang LIU Yanpo CAO 《Asian Agricultural Research》 2019年第11期53-55,共3页
Asparagus officinalis L.is favored by its high health function,but its hybrid seeds are expensive.The amount of seed,seed plumpness and germination rate are related to the production costs of breeding enterprises and ... Asparagus officinalis L.is favored by its high health function,but its hybrid seeds are expensive.The amount of seed,seed plumpness and germination rate are related to the production costs of breeding enterprises and large growers.Therefore,it is necessary to investigate the seed number and thousand kernel weight of A.officinalis L.This study developed a quick and accurate method to measure the seed number and thousand kernel weight of A.officinalis L.using image processing technology.Seed sample of A.officinalis L.was scanned with 200 dpi resolution,and the seed number was then obtained using Image-ProPlus software.After weighing the seeds,thousand kernel weight was finally calculated.By recording‘macro’,the batch processing of the samples can also be realized.This method is simple and accurate,and can greatly save the time of investigation. 展开更多
关键词 ASPARAGUS ofFICINALIS L. SEED number Thousand KERNEL WEIGHT Image processing method
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Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice
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作者 Mohamed Aghyad Al Kabbani Christoph Köhler Hans Zempel 《Neural Regeneration Research》 SCIE CAS 2025年第8期2348-2360,共13页
TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal... TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing enzymes.As impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule dynamics.We therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to microtubules.To investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic littermates.In sum,P301L-TAU results in changes in microtubule PTMs,suggestive of impairment of microtubule stability.This is accompanied by missorting and aggregation of TAU in mice but not in i Neurons.Microtubule PTMs/impairment may be of key importance in tauopathies. 展开更多
关键词 human induced pluripotent stem cell MICROTUBULES P301L pR5 mice TAU TAUOPATHY tubulin code
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