The adsorption of 80S ribosome from rat liver on the surface of lipid monolayers was examined by the electron microscopy (EM) using a negative staining method. The results showed that a large number of ribosomes were ...The adsorption of 80S ribosome from rat liver on the surface of lipid monolayers was examined by the electron microscopy (EM) using a negative staining method. The results showed that a large number of ribosomes were adsorbed on the lipid membrane surfaces containing positively charged SA (octadecylamine), while few ribosomes were observed on surfaces of neutral or negatively charged lipid monolayers. The interaction of ribosome with positively charged monolayers was affected by the composition and ratio of the membrane lipids, and by the ionic strength and pH value of the subphase buffer. The results suggest that ribosome should be adsorbed on the membrane surface through electrostatic force.展开更多
文摘The adsorption of 80S ribosome from rat liver on the surface of lipid monolayers was examined by the electron microscopy (EM) using a negative staining method. The results showed that a large number of ribosomes were adsorbed on the lipid membrane surfaces containing positively charged SA (octadecylamine), while few ribosomes were observed on surfaces of neutral or negatively charged lipid monolayers. The interaction of ribosome with positively charged monolayers was affected by the composition and ratio of the membrane lipids, and by the ionic strength and pH value of the subphase buffer. The results suggest that ribosome should be adsorbed on the membrane surface through electrostatic force.
