This paper proposed a new algorithm to separate leukocytes from cytological image by setting parameters of lightness transformation based on the RGB color space, which can make the targets’ color in different areas. ...This paper proposed a new algorithm to separate leukocytes from cytological image by setting parameters of lightness transformation based on the RGB color space, which can make the targets’ color in different areas. In our procedure, an operator is employed in using color features. According to their histogram distribution of hue component in HSL color space after enhancing the contrast of image in RGB color space, the threshold of segmentation between leukocyte and erythrocyte could be achieved well. Especially, this algorithm is more efficient than monochrome for leukocyte segmentation, and the results of experiments show that it provides a good tool for cytological image, which can increase accuracy of segmentation of leukocyte.展开更多
Fiber optic Fourier transform white-light inter-fereometry is presented to interrogate the absolute optical path difference of an Maeh-Zehnder interferometer. The phase change of the interferometer caused by scanning ...Fiber optic Fourier transform white-light inter-fereometry is presented to interrogate the absolute optical path difference of an Maeh-Zehnder interferometer. The phase change of the interferometer caused by scanning wavelength can be calculated by a Fourier transform-based phase demodulation technique. A linear output is achieved.展开更多
The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyu) were studied by shading. Compared to the grape grown under natural light in...The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyu) were studied by shading. Compared to the grape grown under natural light intensity environment, the net photosynthetic rate diurnal variation curve of experimental plants grown in weak light intensity environment was remarkably lower. Its leaf and stem biomass ratio increased when light intensity decreased, the root and new shoot biomass ratio showed an opposite trend. The 14 C-photoassimilates was mostly distributed to young leaves and stem, a little was distributed to root. The metabolism of 14C-photoassimilates distributed to the entire grape body were also changed under weak light intensity environment.展开更多
Understanding chemical transformations of contaminants and the resulting products is extremely important in devising proper monitoring methods for such contaminants and in assessing potential human exposure to the tra...Understanding chemical transformations of contaminants and the resulting products is extremely important in devising proper monitoring methods for such contaminants and in assessing potential human exposure to the transformation products in the environment. Ultraviolet (UV) light from the sun can induce various photochemical transformations of contaminants in the environment. Alkylnaphthalenes are light-molecular-weight polycyclic aromatic hydrocarbons (PAHs) which are one of the most widespread organic pollutants present in ambient air as a result of a variety of incomplete combustion sources. In this study, 1-methylnapthalene,a typical example of an alkylnaphthalene, was subjected to UV irradiation to investigate its transformation in the presence and absence of air. Twenty-one products were detected in the reaction mixtures. Some photo-oxidation products were identified, including both ring-opened and ring-retained oxygenated compounds, such as 1-naphthaldehyde, 1-naphthoic acid, 1-naphthalenemethanol and phthalic anhydride. Although dimeric products were observed in the presence of air, more were found in the presence of helium or argon gas, indicating a different photo-oxidation pathway from those commonly observed in other media, such as water. Under just 48 hours of exposure to the UV light in the presence of air, three major products were formed with a production yield of about 10% each. Compared to 1-methylnapthalene, the UV induced transformation products observed in this study are more volatile, acidic, water soluble or toxic. The formation of these products may significantly change our understanding of the risks assessed solely from the parent compound in contaminants research and supports the inclusion of airborne transformations of the parent compound in risk assessment.展开更多
AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor...AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.METHODSThree groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.RESULTSThe bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSIONThe expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.展开更多
Some authors have developed a few methods of measuring wave slopes based on light refraction, including the measurement method via the distribution of light intensity or color under water. A new method based on light ...Some authors have developed a few methods of measuring wave slopes based on light refraction, including the measurement method via the distribution of light intensity or color under water. A new method based on light refraction is specified for the measurement of wave surface elevation in wave flume via imaging technology. A plane painted with black and white stripes is put on the flume floor as an indication plane, which can be arranged easily and cheaply. Compared with the previous methods, the present method is less sensitive to the noise and nonlinear effects of optical process, which can be taken as a digital method. The CCD camera is fixed above the flume with its optical axis arranged vertically to grab the images of stripes modulated by the wave surface. The modulated value can be calculated from the Hilbert transform, and then the wave surface elevation can be obtained. The algorithm and experimental procedure are specified in detail, and some experimental results are provided to show the validity of the present method.展开更多
Based on the current indoor natural ventilation and lighting in the space of traditional residential buildings,this paper starts from the passive-design optimization of the spatial natural ventilation and lighting,and...Based on the current indoor natural ventilation and lighting in the space of traditional residential buildings,this paper starts from the passive-design optimization of the spatial natural ventilation and lighting,and makes quantitative evaluation on the quality of current interior natural ventilation and lighting for two typical residential buildings by three indexes,including wind speed,static wind area ratio and satisfaction ratio about minimum lighting coefficient. Based on that, this paper conducts the passive design optimization, and establishes the quantitative association and reevaluation among the passive reformation design, natural ventilation,and lighting environmental quality,proposing the general strategy for the existing residential buildings to respond to the passive reformation design of the natural ventilation and lighting. The special reconstruction of core functionary space of integration of "the living room + dining room + partial space"is researched,and the redesign for the optimization and replacement of both indoor and outdoor enclosure parts is explored,which is expected to provide practical exploration on the strategies for passive construction of spatial natural environmental quality within a large number of highly-energy-consumed residential buildings in China,as well as the green design of residential buildings.展开更多
文摘This paper proposed a new algorithm to separate leukocytes from cytological image by setting parameters of lightness transformation based on the RGB color space, which can make the targets’ color in different areas. In our procedure, an operator is employed in using color features. According to their histogram distribution of hue component in HSL color space after enhancing the contrast of image in RGB color space, the threshold of segmentation between leukocyte and erythrocyte could be achieved well. Especially, this algorithm is more efficient than monochrome for leukocyte segmentation, and the results of experiments show that it provides a good tool for cytological image, which can increase accuracy of segmentation of leukocyte.
基金supported by the Program for New Century Excellent Talents (NCET) in the University of China and National 863 Program under Grant No. 2008AA04Z406.
文摘Fiber optic Fourier transform white-light inter-fereometry is presented to interrogate the absolute optical path difference of an Maeh-Zehnder interferometer. The phase change of the interferometer caused by scanning wavelength can be calculated by a Fourier transform-based phase demodulation technique. A linear output is achieved.
基金supported by the National Natural Science Foundation of China(30070531).
文摘The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyu) were studied by shading. Compared to the grape grown under natural light intensity environment, the net photosynthetic rate diurnal variation curve of experimental plants grown in weak light intensity environment was remarkably lower. Its leaf and stem biomass ratio increased when light intensity decreased, the root and new shoot biomass ratio showed an opposite trend. The 14 C-photoassimilates was mostly distributed to young leaves and stem, a little was distributed to root. The metabolism of 14C-photoassimilates distributed to the entire grape body were also changed under weak light intensity environment.
文摘Understanding chemical transformations of contaminants and the resulting products is extremely important in devising proper monitoring methods for such contaminants and in assessing potential human exposure to the transformation products in the environment. Ultraviolet (UV) light from the sun can induce various photochemical transformations of contaminants in the environment. Alkylnaphthalenes are light-molecular-weight polycyclic aromatic hydrocarbons (PAHs) which are one of the most widespread organic pollutants present in ambient air as a result of a variety of incomplete combustion sources. In this study, 1-methylnapthalene,a typical example of an alkylnaphthalene, was subjected to UV irradiation to investigate its transformation in the presence and absence of air. Twenty-one products were detected in the reaction mixtures. Some photo-oxidation products were identified, including both ring-opened and ring-retained oxygenated compounds, such as 1-naphthaldehyde, 1-naphthoic acid, 1-naphthalenemethanol and phthalic anhydride. Although dimeric products were observed in the presence of air, more were found in the presence of helium or argon gas, indicating a different photo-oxidation pathway from those commonly observed in other media, such as water. Under just 48 hours of exposure to the UV light in the presence of air, three major products were formed with a production yield of about 10% each. Compared to 1-methylnapthalene, the UV induced transformation products observed in this study are more volatile, acidic, water soluble or toxic. The formation of these products may significantly change our understanding of the risks assessed solely from the parent compound in contaminants research and supports the inclusion of airborne transformations of the parent compound in risk assessment.
文摘AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.METHODSThree groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.RESULTSThe bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSIONThe expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.
基金This research was sponsored by the National Science Foundation for Distinguished Young Scholars under contract No.50125924by the National Natural Science Foundation of China under contract Nos 50379001 and 10332050.
文摘Some authors have developed a few methods of measuring wave slopes based on light refraction, including the measurement method via the distribution of light intensity or color under water. A new method based on light refraction is specified for the measurement of wave surface elevation in wave flume via imaging technology. A plane painted with black and white stripes is put on the flume floor as an indication plane, which can be arranged easily and cheaply. Compared with the previous methods, the present method is less sensitive to the noise and nonlinear effects of optical process, which can be taken as a digital method. The CCD camera is fixed above the flume with its optical axis arranged vertically to grab the images of stripes modulated by the wave surface. The modulated value can be calculated from the Hilbert transform, and then the wave surface elevation can be obtained. The algorithm and experimental procedure are specified in detail, and some experimental results are provided to show the validity of the present method.
基金Sponsored by the Key Project of National Natural Science Foundation of China (Grant No.51138004)the South China Key Laboratory Fund (Grant No.20121458321)the Architect Design on Energy-saving Residence in Shanghai (Grant No.08-2A-0183-zong)
文摘Based on the current indoor natural ventilation and lighting in the space of traditional residential buildings,this paper starts from the passive-design optimization of the spatial natural ventilation and lighting,and makes quantitative evaluation on the quality of current interior natural ventilation and lighting for two typical residential buildings by three indexes,including wind speed,static wind area ratio and satisfaction ratio about minimum lighting coefficient. Based on that, this paper conducts the passive design optimization, and establishes the quantitative association and reevaluation among the passive reformation design, natural ventilation,and lighting environmental quality,proposing the general strategy for the existing residential buildings to respond to the passive reformation design of the natural ventilation and lighting. The special reconstruction of core functionary space of integration of "the living room + dining room + partial space"is researched,and the redesign for the optimization and replacement of both indoor and outdoor enclosure parts is explored,which is expected to provide practical exploration on the strategies for passive construction of spatial natural environmental quality within a large number of highly-energy-consumed residential buildings in China,as well as the green design of residential buildings.
