[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guan...[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.展开更多
Leaf area index (LAI) of natural vegetation is recognized as the most important variable for measuring vegetation structure over large areas, and for relating it to energy and mass exchange, which has been successfull...Leaf area index (LAI) of natural vegetation is recognized as the most important variable for measuring vegetation structure over large areas, and for relating it to energy and mass exchange, which has been successfully estimated from satellite resolution sensors. In this paper, according to the statistical analysis based on a lot of forest plots, the mathematical models of LAI distribution patterns in the hydro thermal spaces for five coniferous forest types in China were established. For the cold temperate larch forests growing in the dry and cold climate, their LAI increases with the increasing of warm index and precipitation in the way of hyperbolic quadratic surface. For the cold temperate spruce fir forests and temperate Pinus tabulaeformis forests, their LAI is negatively related to the annual mean air temperature in the way of the natural exponential curve, in order to adapt to the water oppressed environments. For the subtropical Pinus massoniana forests and Cunninghamia lanceolata forests growing in the warm and moist climate, their LAI is related to the annual mean air temperature in the way of the parabolic quadratic curve.展开更多
This study aimed to demonstrate change in spatial correlation between Korean pine （Pinus koraiensis Sieb. et Zucc.） and three rare species, and change in spatial distribution of four species in response to a range o...This study aimed to demonstrate change in spatial correlation between Korean pine （Pinus koraiensis Sieb. et Zucc.） and three rare species, and change in spatial distribution of four species in response to a range of selective cutting intensities. We sampled three plots of mixed Korean pine and broad-leaf forest in Lushuihe Forestry Bureau of Jilin province, China. Plot 1, a control, was unlogged Korean pine broad-leaf forest. In plots 2 and 3, Korean pine was selectively cut at 15 and 30 % intensity, respectively, in the 1970s. Other species were rarely cut. We used point-pattern analysis to research the spatial distributions of four tree species and quantify spatial correlations between Korean pine and the other three species, Amur linden （Tilia amurensis Rupr.）, Manchurian ash （Fraxinus mandshurica Rupr.）, and Mongolian oak （Quercus mongolica Fisch.） in all three plots. The results of the study show that selective cutting at 15 % intensity did not significantly change either the species spatial patterns or the spatial correlation between Korean pine and broadleaf species. Selective cutting at 30 % intensity slightly affected the growth of Korean pine and valuable species in forest communities, and the effect was considered nondestructive and recoverable.展开更多
The pharmaceutical industry increasingly values medicinal plants due to their perceived safety and costeffectiveness compared to modern drugs.Throughout the extensive history of medicinal plant usage,various plant par...The pharmaceutical industry increasingly values medicinal plants due to their perceived safety and costeffectiveness compared to modern drugs.Throughout the extensive history of medicinal plant usage,various plant parts,including flowers,leaves,and roots,have been acknowledged for their healing properties and employed in plant identification.Leaf images,however,stand out as the preferred and easily accessible source of information.Manual plant identification by plant taxonomists is intricate,time-consuming,and prone to errors,relying heavily on human perception.Artificial intelligence(AI)techniques offer a solution by automating plant recognition processes.This study thoroughly examines cutting-edge AI approaches for leaf image-based plant identification,drawing insights from literature across renowned repositories.This paper critically summarizes relevant literature based on AI algorithms,extracted features,and results achieved.Additionally,it analyzes extensively used datasets in automated plant classification research.It also offers deep insights into implemented techniques and methods employed for medicinal plant recognition.Moreover,this rigorous review study discusses opportunities and challenges in employing these AI-based approaches.Furthermore,in-depth statistical findings and lessons learned from this survey are highlighted with novel research areas with the aim of offering insights to the readers and motivating new research directions.This review is expected to serve as a foundational resource for future researchers in the field of AI-based identification of medicinal plants.展开更多
为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标...为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标准为影响茶鲜叶转录和代谢轮廓的重要因素,其中CK、TM和MM的转录与代谢轮廓相互间均有良好的模式区分。差异表达基因与差异代谢物的富集和关联分析结果表明,TM vs CK与MM vs TM的差异表达基因在分子功能、细胞组分和生物过程方面存在不同的GO功能富集模式,且前者拥有较多的生物过程和较少的细胞组分;“植物次生代谢产物的生物合成”、“精氨酸和脯氨酸代谢”与“类胡萝卜素生物合成”、“苯丙素的生物合成”、“甘氨酸、丝氨酸和苏氨酸代谢”分别是TM vsCK、MM vs TM在2种组学分析中的前20条共有KEGG富集代谢通路。TM vs CK中L-苯丙氨酸、反式肉桂酸和4-胍基丁酸丰度的显著降低,以及MM vs TM中胆碱丰度的显著提高和L-苯丙氨酸、L-色氨酸、L-高丝氨酸丰度的显著降低均与相应共富集代谢通路中多个基因的显著上调或下调表达密切相关。此外,MM vs TM中脱落酸丰度的显著提高与“类胡萝卜素生物合成”通路中紫黄质脱环氧化酶和番茄红素β-环化酶基因的显著上调表达高度相关。这可为阐明‘茗科1号’茶鲜叶生物学特征及其多茶类兼制特性提供参考依据。展开更多
脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的...脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的功能尚不清楚。筛选对干热双重胁迫联合响应的文采报春苣苔DREB2同源基因,可为苦苣苔抗逆种质的创制提供新的基因储备。该研究首先根据文采报春苣苔转录组的序列信息设计特异引物,以gDNA和cDNA为模板分离PwDREB2s基因,然后通过生物信息学方法,对氨基酸序列、系统进化关系及保守基序进行分析,再使用半定量RT-PCR(sqRT-PCR)分析PwDREB2s基因在低温、热激、模拟干旱(渗透)、高盐、外源ABA及氧化等单一胁迫下的表达模式,最后选择能同时应答模拟干旱与热激的成员,利用实时荧光定量PCR(qRT-PCR)检测其在单一和复合干热处理中的转录本水平。结果表明:(1)分离获得了8条缺少内含子的PwDREB2s基因,编码198~386个氨基酸,拥有AP2/ERF结构域、核定位信号或转录激活域等典型特征序列;(2)PwDREB2A/2AL1/2AL2、PwDREB2D/2DL和PwDREB2F转录因子被分别归入A-2亚组的亚型1、亚型2和亚型3,PwDREB2EL1/2EL2转录因子则被归入A-6亚组;(3)SqRT-PCR分析表明PwDREB2s基因表达受多种单一胁迫的诱导,其中PwDREB2A/2AL1/2AL2/2D基因能同时应答模拟干旱与热激胁迫;(4)qRT-PCR结果揭示了在液体培养条件下,PwDREB2D基因对干热处理的应答具有组织特异性,仅能在根状茎中被强烈诱导。在土壤栽培条件下,与对照相比时,除PwDREB2D基因以外,PwDREB2A/2AL1/2AL2基因在自然干旱-热激复合胁迫中的转录反应显著增强。与单一的自然干旱和高温处理相比时,PwDREB2AL1/2AL2基因在自然干旱-热激复合胁迫中的表达水平显著升高。由此可见,PwDREB2AL1/2AL2基因在文采报春苣苔干热复合胁迫应答中可能发挥了重要的调控作用。展开更多
In forest variety registration, visual traits of the plants appearance are widely used to discern different tree species. The new recognition system of leaf image strategy which based on neural network established to ...In forest variety registration, visual traits of the plants appearance are widely used to discern different tree species. The new recognition system of leaf image strategy which based on neural network established to administrate a hierarchical list of leaf images, some sorts of edge detection can be performed to identify the individual tokens of every image and the frame of the leaf can be got to differentiate the tree species. An approach based on back-propagation neuronal network is proposed and the programming language for the implementation is also Riven by using Java. The numerical simulations results have shown that the proposed leaf strategt is effective and feasible.展开更多
Comprehensive information on geographic patterns of leaf morphological traits in Chinese forests is still scarce.To explore the spatial patterns of leaf traits,we investigated leaf area(LA),leaf thickness(LT),specific...Comprehensive information on geographic patterns of leaf morphological traits in Chinese forests is still scarce.To explore the spatial patterns of leaf traits,we investigated leaf area(LA),leaf thickness(LT),specific leaf area(SLA),and leaf dry matter content(LDMC) across 847 species from nine typical forests along the North-South Transect of Eastern China(NSTEC) between July and August 2013,and also calculated the community weighted means(CWM) of leaf traits by determining the relative dominance of each species.Our results showed that,for all species,the means(± SE) of LA,LT,SLA,and LDMC were 2860.01 ± 135.37 mm2,0.17 ± 0.003 mm,20.15 ± 0.43 m2 kg–1,and 316.73 ± 3.81 mg g–1,respectively.Furthermore,latitudinal variation in leaf traits differed at the species and community levels.Generally,at the species level,SLA increased and LDMC decreased as latitude increased,whereas no clear latitudinal trends among LA or LT were found,which could be the result of shifts in plant functional types.When scaling up to the community level,more significant spatial patterns of leaf traits were observed(R2 = 0.46–0.71),driven by climate and soil N content.These results provided synthetic data compilation and analyses to better parameterize complex ecological models in the future,and emphasized the importance of scaling-up when studying the biogeographic patterns of plant traits.展开更多
Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides （ introduced, local wild, domesticated wild populations of different years）. The re...Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides （ introduced, local wild, domesticated wild populations of different years）. The results showed that four populations varied in different degrees in terms of tiller height, leaf biomass, and leaf biomass ratio. Leaf length, leaf width, and ratio of leaf width to length of different populations also varied, but had similar power-function change regularity, and showed converging leaf biomass growth pattern and leaf growth process.展开更多
An experiment was conducted on Fluvisols of Awassa for two consecutive years （2005-2006） to determine effects of planting pattern and plant density on dry matter accumulation, nodulation, protein and oil content in ...An experiment was conducted on Fluvisols of Awassa for two consecutive years （2005-2006） to determine effects of planting pattern and plant density on dry matter accumulation, nodulation, protein and oil content in early and late maturing soybean varieties. Results indicated that Awassa-95 variety produced significantly higher （P 〈 0.05） number of nodules/plant （NDN）, nodule dry matter （NDM） and leaf dry matter （LDM at R2 （mid flowering） stage of soybean growth than that of variety Belessa-95）. Similarly, variety Awassa-95 （45%） produced significantly higher protein content than variety Belessa-95 （40%）. However, variety Belessa-95 accumulated significantly higher （P 〈 0.01） dry matter in straw, grain and total biomass at R7 （physiological maturity） stage of soybean growth than variety Awassa-95. Similarly, oil content of variety Belessa-95 （18.1%） was significantly （P 〈 0.05） higher than that of variety Awassa-95 （15.9%）. Equidistant rows produced significantly higher （P 〈 0.05） NDM than either rectangular or paired rows. Moreover, soybean plants grown in both rectangular and equidistant rows produced significantly higher （P 〈 0.01） straw dry matter than those grown in paired rows; but, grain dry matter/plant （GDM） was significantly higher （P 〈 0.01） paired and rectangular rows compared to equidistant rows. Plant density also affected the per plant GDM production as it was significantly higher （P 〈 0.01） in 20 and 30 plants/m2 than higher plant densities （40 and 50 plants/m2）. However, dry matter and yield components had strong negative association with protein content. In fact, strong positive correlation （R 〉 0.600） occurred between grain yield and its components with dry matter components at R2 （stem dry matter （SDM）, leaf dry matter （LDM） and stem ＋ nodule ＋ leaf dry matter together known as TDM） and straw dry matter at R7 in both varieties. This study depicted that soybean plants that produce higher dry matter components at R2 would probably produce more straw dry matter, greater grain yield components and higher grain yield dry matter at later stages.展开更多
Mitogen-activated protein kinase （MAPK） cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress re...Mitogen-activated protein kinase （MAPK） cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses, and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activated by their upstream MAP kinase kinases （MKKs） AtMKK4 and AtMKK5 in response to biotic and abiotic stress. In addition, they were identified as key regulators of stomatal development and patterning. AtMPKIO has long been considered as a pseudo-gene, derived from a gene duplication of AtMPK6. Here we show that AtMPKIO is expressed highly but very transiently in seedlings and at sites of local auxin maxima leaves. MPK10 encodes a functional kinase and interacts with the upstream MAP kinase kinase （MAPKK） AtMKK2. mpklO mutants are delayed in flowering in long-day conditions and in continuous light. Moreover, cotyledons of mpk10 and mkk2 mutants have reduced vein complexity, which can be reversed by inhibiting polar auxin transport （PAT）. Auxin does not affect AtMPKIO expression while treatment with the PAT inhibitor HFCA extends the expression in leaves and reverses the mpklO mutant phenotype. These results suggest that the AtMKK2-AtMPK10 MAPK module regulates venation complexity by altering PAT efficiency.展开更多
基金Supported by Guangxi Key Laboratory of Zhuang and Yao Medicine(GuiKeJiZi 201432)Zhuang and Yao Medicine Collaborative Innovation Center(GuiJiaoKeYan 201320)+3 种基金Guangxi Young Talent Project of Guangxi University of Chinese Medicine(2022C030)Ethnic Medicine Resources and Application Engineering Research Center in Guangxi Zhuang Autonomous Region(GuiFaGaiGaoJiHan 20202605)Key Discipline Project of Traditional Chinese Medicine in Guangxi—Zhuang Medicine(GZXK-Z-20-64)Guangxi First-class Discipline of Traditional Chinese Medicine—Ethnopharmacology(GuiJiaoKeYan 201812).
文摘[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.
文摘Leaf area index (LAI) of natural vegetation is recognized as the most important variable for measuring vegetation structure over large areas, and for relating it to energy and mass exchange, which has been successfully estimated from satellite resolution sensors. In this paper, according to the statistical analysis based on a lot of forest plots, the mathematical models of LAI distribution patterns in the hydro thermal spaces for five coniferous forest types in China were established. For the cold temperate larch forests growing in the dry and cold climate, their LAI increases with the increasing of warm index and precipitation in the way of hyperbolic quadratic surface. For the cold temperate spruce fir forests and temperate Pinus tabulaeformis forests, their LAI is negatively related to the annual mean air temperature in the way of the natural exponential curve, in order to adapt to the water oppressed environments. For the subtropical Pinus massoniana forests and Cunninghamia lanceolata forests growing in the warm and moist climate, their LAI is related to the annual mean air temperature in the way of the parabolic quadratic curve.
基金funded by China National Science and Technology Support Program(Grant No.2012BAD21B02)
文摘This study aimed to demonstrate change in spatial correlation between Korean pine （Pinus koraiensis Sieb. et Zucc.） and three rare species, and change in spatial distribution of four species in response to a range of selective cutting intensities. We sampled three plots of mixed Korean pine and broad-leaf forest in Lushuihe Forestry Bureau of Jilin province, China. Plot 1, a control, was unlogged Korean pine broad-leaf forest. In plots 2 and 3, Korean pine was selectively cut at 15 and 30 % intensity, respectively, in the 1970s. Other species were rarely cut. We used point-pattern analysis to research the spatial distributions of four tree species and quantify spatial correlations between Korean pine and the other three species, Amur linden （Tilia amurensis Rupr.）, Manchurian ash （Fraxinus mandshurica Rupr.）, and Mongolian oak （Quercus mongolica Fisch.） in all three plots. The results of the study show that selective cutting at 15 % intensity did not significantly change either the species spatial patterns or the spatial correlation between Korean pine and broadleaf species. Selective cutting at 30 % intensity slightly affected the growth of Korean pine and valuable species in forest communities, and the effect was considered nondestructive and recoverable.
文摘The pharmaceutical industry increasingly values medicinal plants due to their perceived safety and costeffectiveness compared to modern drugs.Throughout the extensive history of medicinal plant usage,various plant parts,including flowers,leaves,and roots,have been acknowledged for their healing properties and employed in plant identification.Leaf images,however,stand out as the preferred and easily accessible source of information.Manual plant identification by plant taxonomists is intricate,time-consuming,and prone to errors,relying heavily on human perception.Artificial intelligence(AI)techniques offer a solution by automating plant recognition processes.This study thoroughly examines cutting-edge AI approaches for leaf image-based plant identification,drawing insights from literature across renowned repositories.This paper critically summarizes relevant literature based on AI algorithms,extracted features,and results achieved.Additionally,it analyzes extensively used datasets in automated plant classification research.It also offers deep insights into implemented techniques and methods employed for medicinal plant recognition.Moreover,this rigorous review study discusses opportunities and challenges in employing these AI-based approaches.Furthermore,in-depth statistical findings and lessons learned from this survey are highlighted with novel research areas with the aim of offering insights to the readers and motivating new research directions.This review is expected to serve as a foundational resource for future researchers in the field of AI-based identification of medicinal plants.
文摘为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标准为影响茶鲜叶转录和代谢轮廓的重要因素,其中CK、TM和MM的转录与代谢轮廓相互间均有良好的模式区分。差异表达基因与差异代谢物的富集和关联分析结果表明,TM vs CK与MM vs TM的差异表达基因在分子功能、细胞组分和生物过程方面存在不同的GO功能富集模式,且前者拥有较多的生物过程和较少的细胞组分;“植物次生代谢产物的生物合成”、“精氨酸和脯氨酸代谢”与“类胡萝卜素生物合成”、“苯丙素的生物合成”、“甘氨酸、丝氨酸和苏氨酸代谢”分别是TM vsCK、MM vs TM在2种组学分析中的前20条共有KEGG富集代谢通路。TM vs CK中L-苯丙氨酸、反式肉桂酸和4-胍基丁酸丰度的显著降低,以及MM vs TM中胆碱丰度的显著提高和L-苯丙氨酸、L-色氨酸、L-高丝氨酸丰度的显著降低均与相应共富集代谢通路中多个基因的显著上调或下调表达密切相关。此外,MM vs TM中脱落酸丰度的显著提高与“类胡萝卜素生物合成”通路中紫黄质脱环氧化酶和番茄红素β-环化酶基因的显著上调表达高度相关。这可为阐明‘茗科1号’茶鲜叶生物学特征及其多茶类兼制特性提供参考依据。
文摘脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的功能尚不清楚。筛选对干热双重胁迫联合响应的文采报春苣苔DREB2同源基因,可为苦苣苔抗逆种质的创制提供新的基因储备。该研究首先根据文采报春苣苔转录组的序列信息设计特异引物,以gDNA和cDNA为模板分离PwDREB2s基因,然后通过生物信息学方法,对氨基酸序列、系统进化关系及保守基序进行分析,再使用半定量RT-PCR(sqRT-PCR)分析PwDREB2s基因在低温、热激、模拟干旱(渗透)、高盐、外源ABA及氧化等单一胁迫下的表达模式,最后选择能同时应答模拟干旱与热激的成员,利用实时荧光定量PCR(qRT-PCR)检测其在单一和复合干热处理中的转录本水平。结果表明:(1)分离获得了8条缺少内含子的PwDREB2s基因,编码198~386个氨基酸,拥有AP2/ERF结构域、核定位信号或转录激活域等典型特征序列;(2)PwDREB2A/2AL1/2AL2、PwDREB2D/2DL和PwDREB2F转录因子被分别归入A-2亚组的亚型1、亚型2和亚型3,PwDREB2EL1/2EL2转录因子则被归入A-6亚组;(3)SqRT-PCR分析表明PwDREB2s基因表达受多种单一胁迫的诱导,其中PwDREB2A/2AL1/2AL2/2D基因能同时应答模拟干旱与热激胁迫;(4)qRT-PCR结果揭示了在液体培养条件下,PwDREB2D基因对干热处理的应答具有组织特异性,仅能在根状茎中被强烈诱导。在土壤栽培条件下,与对照相比时,除PwDREB2D基因以外,PwDREB2A/2AL1/2AL2基因在自然干旱-热激复合胁迫中的转录反应显著增强。与单一的自然干旱和高温处理相比时,PwDREB2AL1/2AL2基因在自然干旱-热激复合胁迫中的表达水平显著升高。由此可见,PwDREB2AL1/2AL2基因在文采报春苣苔干热复合胁迫应答中可能发挥了重要的调控作用。
基金Foundation project： This paper was supported by National Natural Science Foundation of China （No. 30371126）.
文摘In forest variety registration, visual traits of the plants appearance are widely used to discern different tree species. The new recognition system of leaf image strategy which based on neural network established to administrate a hierarchical list of leaf images, some sorts of edge detection can be performed to identify the individual tokens of every image and the frame of the leaf can be got to differentiate the tree species. An approach based on back-propagation neuronal network is proposed and the programming language for the implementation is also Riven by using Java. The numerical simulations results have shown that the proposed leaf strategt is effective and feasible.
基金National Natural Science Foundation of China,No.31290221,No.31470506Chinese Academy of Sciences Strategic Priority Research Program,No.XDA05050702Program for Kezhen Distinguished Talents in Institute of Geographic Sciences and Natural Resources Research,CAS,No.2013RC102
文摘Comprehensive information on geographic patterns of leaf morphological traits in Chinese forests is still scarce.To explore the spatial patterns of leaf traits,we investigated leaf area(LA),leaf thickness(LT),specific leaf area(SLA),and leaf dry matter content(LDMC) across 847 species from nine typical forests along the North-South Transect of Eastern China(NSTEC) between July and August 2013,and also calculated the community weighted means(CWM) of leaf traits by determining the relative dominance of each species.Our results showed that,for all species,the means(± SE) of LA,LT,SLA,and LDMC were 2860.01 ± 135.37 mm2,0.17 ± 0.003 mm,20.15 ± 0.43 m2 kg–1,and 316.73 ± 3.81 mg g–1,respectively.Furthermore,latitudinal variation in leaf traits differed at the species and community levels.Generally,at the species level,SLA increased and LDMC decreased as latitude increased,whereas no clear latitudinal trends among LA or LT were found,which could be the result of shifts in plant functional types.When scaling up to the community level,more significant spatial patterns of leaf traits were observed(R2 = 0.46–0.71),driven by climate and soil N content.These results provided synthetic data compilation and analyses to better parameterize complex ecological models in the future,and emphasized the importance of scaling-up when studying the biogeographic patterns of plant traits.
基金Sponsored by National Spark Program(2012GA780024)Program of Marginal Tropical Characteristic Plant Resources Development Center of Guangdong Universities and Colleges(GCZX-B1002)
文摘Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides （ introduced, local wild, domesticated wild populations of different years）. The results showed that four populations varied in different degrees in terms of tiller height, leaf biomass, and leaf biomass ratio. Leaf length, leaf width, and ratio of leaf width to length of different populations also varied, but had similar power-function change regularity, and showed converging leaf biomass growth pattern and leaf growth process.
文摘An experiment was conducted on Fluvisols of Awassa for two consecutive years （2005-2006） to determine effects of planting pattern and plant density on dry matter accumulation, nodulation, protein and oil content in early and late maturing soybean varieties. Results indicated that Awassa-95 variety produced significantly higher （P 〈 0.05） number of nodules/plant （NDN）, nodule dry matter （NDM） and leaf dry matter （LDM at R2 （mid flowering） stage of soybean growth than that of variety Belessa-95）. Similarly, variety Awassa-95 （45%） produced significantly higher protein content than variety Belessa-95 （40%）. However, variety Belessa-95 accumulated significantly higher （P 〈 0.01） dry matter in straw, grain and total biomass at R7 （physiological maturity） stage of soybean growth than variety Awassa-95. Similarly, oil content of variety Belessa-95 （18.1%） was significantly （P 〈 0.05） higher than that of variety Awassa-95 （15.9%）. Equidistant rows produced significantly higher （P 〈 0.05） NDM than either rectangular or paired rows. Moreover, soybean plants grown in both rectangular and equidistant rows produced significantly higher （P 〈 0.01） straw dry matter than those grown in paired rows; but, grain dry matter/plant （GDM） was significantly higher （P 〈 0.01） paired and rectangular rows compared to equidistant rows. Plant density also affected the per plant GDM production as it was significantly higher （P 〈 0.01） in 20 and 30 plants/m2 than higher plant densities （40 and 50 plants/m2）. However, dry matter and yield components had strong negative association with protein content. In fact, strong positive correlation （R 〉 0.600） occurred between grain yield and its components with dry matter components at R2 （stem dry matter （SDM）, leaf dry matter （LDM） and stem ＋ nodule ＋ leaf dry matter together known as TDM） and straw dry matter at R7 in both varieties. This study depicted that soybean plants that produce higher dry matter components at R2 would probably produce more straw dry matter, greater grain yield components and higher grain yield dry matter at later stages.
文摘Mitogen-activated protein kinase （MAPK） cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses, and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activated by their upstream MAP kinase kinases （MKKs） AtMKK4 and AtMKK5 in response to biotic and abiotic stress. In addition, they were identified as key regulators of stomatal development and patterning. AtMPKIO has long been considered as a pseudo-gene, derived from a gene duplication of AtMPK6. Here we show that AtMPKIO is expressed highly but very transiently in seedlings and at sites of local auxin maxima leaves. MPK10 encodes a functional kinase and interacts with the upstream MAP kinase kinase （MAPKK） AtMKK2. mpklO mutants are delayed in flowering in long-day conditions and in continuous light. Moreover, cotyledons of mpk10 and mkk2 mutants have reduced vein complexity, which can be reversed by inhibiting polar auxin transport （PAT）. Auxin does not affect AtMPKIO expression while treatment with the PAT inhibitor HFCA extends the expression in leaves and reverses the mpklO mutant phenotype. These results suggest that the AtMKK2-AtMPK10 MAPK module regulates venation complexity by altering PAT efficiency.
