Knowledge of the dynamic modulus of bituminous mixtures is practical and theoretically meaningful in pavement design,construction,and monitoring.The tests in the laboratory for the determination of asphalt concrete(AC...Knowledge of the dynamic modulus of bituminous mixtures is practical and theoretically meaningful in pavement design,construction,and monitoring.The tests in the laboratory for the determination of asphalt concrete(AC)moduli include the resilient modulus through the indirect tensile test(EN 12697-26),the complex modulus through the four point bending beam(EN 12697-26),the asphalt mixture performance tester(AMPT)and the simple performance tester(SPT)(AASHTO T342).Unfortunately,the tests above are time-consuming and quite expensive.On the other hand,the standard ASTM E1876 for resonant tests applies only to very thin(stocky)cylindrical samples(with a thickness-to-radius ratio,t/r,lower than 0.5)while the typical AC samples produced in the laboratory do not satisfy the ASTM E1876 requirements.Consequently,the main objective of this study is to set up and implement a tentative method to extend the range of applicability of the standard ASTM E1876 to common AC samples.The methodology was to carry out resonant tests on slender samples and to cut each of them into stocky samples(these latter complying with ASTM E1876 requirements in terms of t/r),deriving the master curve per material.These master curves allowed for deriving the value of the dynamic modulus for the given selected sample under its particular test conditions during the resonant test(i.e.,temperature and frequency).Consequently,simplified formulae were provided for AC samples.Results were compared to Witczak's estimates.These formulae provide an approximate tool to carry out low-cost and high-speed inferences at the laboratory stage on common AC samples,whatever their thickness is.Additional studies are needed to investigate the reliability of the method further and reduce uncertainties.展开更多
Acute respiratory tract infection</span><span style="font-family:""><span style="font-family:Verdana;"> (ARTI) in children is the most common infectious disease in childhood,...Acute respiratory tract infection</span><span style="font-family:""><span style="font-family:Verdana;"> (ARTI) in children is the most common infectious disease in childhood, and its pathogens include viruses, bacteria and fungi, mycoplasma, chlamydia and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more clinical attention. The clinical diagnosis and treatment characteristics of acute respira</span><span style="font-family:Verdana;">tory infections in children and the research of clinical laboratory detection </span><span style="font-family:Verdana;">methods have also been continuously developed. The author collected refer</span><span style="font-family:Verdana;">ences to review the clinical features and new developments in laboratory</span><span style="font-family:Verdana;"> testing of acute respiratory tract infection in children.展开更多
Acute respiratory infection in children (ARTI) is the most common childhood infectious disease, and its pathogens include bacteria, fungi, viruses, chlamydia, mycoplasma and rickettsia. In recent years, with the conti...Acute respiratory infection in children (ARTI) is the most common childhood infectious disease, and its pathogens include bacteria, fungi, viruses, chlamydia, mycoplasma and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more attention from clinicians. The clinical diagnosis and treatment of acute respiratory infections in children and the research of laboratory detection methods have also been continuously developed. The manuscript presents a review of progress in the clinical diagnosis, treatment and laboratory testing of acute respiratory infections in children by collecting references.展开更多
Objective: to discuss the related factors affecting the quality control of pharmaceutical microorganism testing laboratories and their countermeasures. Methods: the computer was used to collect the occurrence of detec...Objective: to discuss the related factors affecting the quality control of pharmaceutical microorganism testing laboratories and their countermeasures. Methods: the computer was used to collect the occurrence of detection bias samples in the pharmaceutical microbiology laboratory of our hospital. The retrieval period was set as June 2019 to August 2020. The causes of the bias samples were sorted out and analyzed, and the corresponding measures were formulated. Results: after investigation and statistics, 109 samples of testing deviation occurred in our hospital's pharmaceutical microorganism testing laboratory, among which the non-compliance of personnel's operation was the main reason, accounting for 34.86%. The second was the testing deviation caused by equipment problems, testing methods and standard problems, accounting for 26.61% and 14.68% respectively. Conclusion: the factors affecting the quality control management of pharmaceutical microorganism testing laboratories involve various aspects, and the laboratories should pay attention to them and formulate specific measures to prevent specific problems in order to reduce the occurrence of testing deviation events and improve the quality control management of laboratories.展开更多
In view of the difficulties about school-enterprise cooperation on food safety testing laboratory reform,the current training program,teaching methods and teaching forms are not suitable for the new platform.This pape...In view of the difficulties about school-enterprise cooperation on food safety testing laboratory reform,the current training program,teaching methods and teaching forms are not suitable for the new platform.This paper provides new ideas and modes to solve these problems,such as integrating the resource elements between school and enterprise,establishing new platforms with the help of external force and innovating the cooperation modes to improve the laboratory,which can fully serve teaching,scientific research and enterprise production.展开更多
The capacity of ground support components which have been affected by corrosion is reduced and may ultimately lead to dynamic failure of the component and the strata. In order to maintain an effective,long-term ground...The capacity of ground support components which have been affected by corrosion is reduced and may ultimately lead to dynamic failure of the component and the strata. In order to maintain an effective,long-term ground support system, significant campaigns of rehabilitation are often required in corrosion affected areas which also expose the workers to hazardous conditions. The most common corrosion protection for steel ground support utilises sacrificial systems such as galvanising. Galvanising has previously been proven to be susceptible to some corrosion processes. Stainless steel is the most effective in resistance to corrosion, but can be cost prohibitive, and its mechanical properties often make it unsuited to use in ground support components. Providing an outer protective plastic coating to bolts has proven to be an effective means of protecting the inner steel bar from corrosion. However, these support systems tend to be susceptible to coating damage, and require post cement grouting to provide full encapsulation. In comparison to a standard bolt/resin system, they can be slow to install and expensive.These systems have also been shown to reduce overall load transfer performance of the bolting system. In order to provide a higher level of corrosion protection whilst maintaining current installation practices and bolting cycle times, Minova has developed the Enduro^(TM)steel ground support range. The Enduro^(TM) range consists of standard Minova steel ground support components which have been treated with a unique coating process. The Enduro^(TM)coating has been tested in the harshest of conditions, in laboratory controlled conditions and in underground trials. It has been proven to effectively resist or completely eliminate the formation of corrosion, even in the most aggressive environments. This paper explains the process and provides the details of the laboratory and underground corrosion performance testing carried out on Enduro^(TM)ground support products.展开更多
This paper describes the development and challenge of HIV/AIDS testing laboratory network and quality assurance system in China. At present,the HIV/AIDS testing laboratories includes three classes,the National AIDS Re...This paper describes the development and challenge of HIV/AIDS testing laboratory network and quality assurance system in China. At present,the HIV/AIDS testing laboratories includes three classes,the National AIDS Reference Laboratory,HIV/AIDS confirmatory laboratories and HIV/AIDS screening laboratories. All of them are accredited by the health authorities,and each class of laboratories take charge of their function strictly according to the "National Management of HIV/AIDS Detection (2006)". A complete quality assurance and quality control system for HIV/AIDS testing has been developed,which includes technical training,strict laboratory monitoring and approval,examination or proficiency testing on HIV/AIDS detection,and quality evaluation and supervision of HIV/AIDS diagnostic kits. Besides conduct the routine anti-HIV antibody test,more and more laboratories began to conduct other tests,such as CD4+ T lymphocyte cell counting,HIV viral load,HIV DNA PCR,genotyping,drug resistance,and HIV-1 recent infection test. The primary challenges faced by the HIV/AIDS testing laboratory network are in the areas of laboratory management and quality control. For example,the provincial PT program is inefficient,the internal quality control is conducted perfunctorily,personnel training can not met the needs of the workplace,which need to be improved.展开更多
BACKGROUND Isotretinoin is a widely prescribed drug for various dermatological conditions,especially acne.Blood tests are routinely performed to monitor its side effects.However,the optimal testing schedule remains co...BACKGROUND Isotretinoin is a widely prescribed drug for various dermatological conditions,especially acne.Blood tests are routinely performed to monitor its side effects.However,the optimal testing schedule remains controversial.AIM To evaluate the laboratory monitoring tests and schedules of patients with acne taking isotretinoin to determine the most efficient testing regimen.METHODS We retrospectively reviewed electronic medical records from the Dermatology Department of Dongguk University Ilsan Hospital from 2005 to 2020 for patients prescribed isotretinoin for acne who underwent monthly blood tests.RESULTS Aspartate aminotransferase(AST),alanine aminotransferase(ALT),and trigly-ceride(TG)levels significantly changed between 5 and 6 mo when the total prescription period and dose variables were considered altogether.The high-density lipoprotein level also significantly changed between 5 and 6 mo.However,low-density lipoprotein(LDL)and total cholesterol levels significantly changed between 1 and 2 mo.CONCLUSION We recommend testing AST,ALT,and TG levels once every 5 to 6 mo.We also suggest testing LDL and total cholesterol levels during the first and second months.展开更多
According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's k...According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.展开更多
The main objective of this study is to develop a new protocol for bench-scale dispersant effectiveness testing adapted for subsea dispersants injection (SSDI). The new approach includes turbulence conditions, dispersa...The main objective of this study is to develop a new protocol for bench-scale dispersant effectiveness testing adapted for subsea dispersants injection (SSDI). The new approach includes turbulence conditions, dispersant injection techniques and quantification of effectiveness, more representative for a SSDI operation. Results from the new system are compared to dispersant effectiveness measured with established laboratory methods, used for screening dispersants for surface application. The most significant result is that the dispersant ranking obtained with the new test relevant for subsea releases was very different compared to the ranking obtained by screening methods used for surface application of dispersants. This strongly indicates that existing standard methods for dispersant effectiveness testing designed for simulating conditions relevant for surface application of dispersants, are less relevant for SSDI effectiveness testing and justify the need for a more relevant method. Such a Dispersant Injection Effectiveness Test (DIET) is suggested and documented in this study.展开更多
Background: Routine pre-operative test, results in significant cost to the hospital and expense for the patient. The cost of a single laboratory test may be negligible but too many tests have increased cost for many n...Background: Routine pre-operative test, results in significant cost to the hospital and expense for the patient. The cost of a single laboratory test may be negligible but too many tests have increased cost for many national health schemes. Most of these patients cannot afford this extra cost thereby delaying surgery and adding up to emergencies. Implementation of change is necessary to reduce economic burden on the patient and enhance patient care. The aim of this study was to determine the economic burden of preoperative laboratory tests on pre-surgical patients in Komfo Anokye Teaching Hospital with a view to making appropriate recommendations that will reduce costs for the patients. Methodology: This was a prospective, cross-sectional study of patients undergoing elective surgery at K.A.T.H from 1st to 31st March 2014. A quantitative technique was used to effectively quantify laboratory results that were contained in a patient’s folder before an elective surgical procedure. Close and open ended questionnaire was developed and answered by reviewing patient’s folders during the pre-anaesthesia assessment. Cost of various laboratory investigations was obtained on request from one private laboratory and the KATH main laboratory. Data were analyzed using Statistical Package of Social Sciences (SSPS) version 22 and Microsoft Excel 2010. Results: The total cost of preoperative laboratory investigations in the study population was GHC 15, 321.19 (GHC 92.85 per individual);and for tests that were not indicated, GHC 3, 266.83 (GHC19.80 per individual). Conclusion: Routine preoperative laboratory tests ordered by Surgeons in Komfo Anokye Teaching Hospital impose an economic burden on the patients. The development of a comprehensive guideline that will help in the ordering of preoperative laboratory investigation will reduce costs for the patients.展开更多
DNA contamination will directly result in wrong conclusion of DNA test. The higher the detection accuracy is, the greater the harmful of contamination is. DNA contamination can be divided into sample contamination and...DNA contamination will directly result in wrong conclusion of DNA test. The higher the detection accuracy is, the greater the harmful of contamination is. DNA contamination can be divided into sample contamination and PCR contamination. It is the most difficult to treat and prevent aerosol contamination of PCR. In order to prevent pollution, dog DNA testing laboratory should be divided into several independent areas, and the researchers should strictly obey the operation rules. In addition, we should design control experiment scientifically to track pollution sources and eliminate the contamination through technical means, once the pollution occurs.展开更多
Background: Request for preoperative laboratory investigations is usually done by surgeons. On some occasions, the patient may come with laboratory investigations that have been requested by the primary physician. Thi...Background: Request for preoperative laboratory investigations is usually done by surgeons. On some occasions, the patient may come with laboratory investigations that have been requested by the primary physician. This occurs in situations where the primary physician saw the patient first and referred to the surgeon. There is usually no indications based on history or physical examination before these laboratory request is done but rather on speculations that the “anaesthetist may require them”. This is done in order to avoid cancellation or delay of cases. The aim of this study was to find out how tests ordered in Komfo Anokye Teaching Hospital (K.A.T.H.) by the surgeons affected the decisions of the anaesthetists in the perioperative management of the patients. Methodology: This was a prospective, cross sectional study of patients undergoing elective surgery at K.A.T.H from 1st to 31st March 2014. A quantitative technique was used to effectively quantify laboratory results that were contained in a patient’s folder before an elective surgical procedure. A close and open-ended questionnaire was developed and answered by reviewing patient’s folders during the pre-anesthesia assessment. Data were analyzed using Statistical Package of Social Sciences (SSPS) version 22. Results: The average age of patients studied was 50 years. Abnormal test results did not influence the anaesthetic management in 70.5% of cases but led to either delay or cancelation of cases or requirement for transfusion of blood or blood products in 29.5% of cases. Conclusion: Preoperative laboratory tests ordered by Surgeons in KomfoAnokye Teaching Hospital do not significantly influence the anaesthetic managements of patients.展开更多
Since the beginning of COVID-19 pandemics many countries were facing challenges with testing capacity recourse limitations. Throughout the waves of the pandemic countries were trying to address the existing constrains...Since the beginning of COVID-19 pandemics many countries were facing challenges with testing capacity recourse limitations. Throughout the waves of the pandemic countries were trying to address the existing constrains exploring solutions to increase the testing capacity with more cost-effective approaches. Pooled methodology was one of the methods which many have validated and used. It is evident that in case of pooled sample testing the sensitivity becomes lower, however the variation highly depends on the pool size as well as the incidence rate at the certain point. Armenia as well as many other countries has adopted regulations for mandatory COVID-19 PCR testing for all the travelers. Current study aimed to explore the efficiency of COVID-19 pooled PCR testing for nasopharyngeal swabs of individuals with no symptoms in a time period with good epidemiological state of the infection. Nasopharingeal swab samples from individuals were collected. The manual extraction of RNAs of samples was performed after pooling up to 5 samples. The pools with Cycle Threshold (CT) of < 37 were considered positive and were retested individually. In total 28,015 samples were grouped in 667 pools of which 57 were positive. The total number of positive samples was 65. The median difference (CT-pool–CT samples) was 2.4 (ranging from–3.0 to 8.9). The correlation of CT of pools and positive samples was positive. The correlation coefficient r = 0.84, P < 0.000, 95% CI range 0.7423 to 0.9243). The total economic saving when using pools compared to the individual testing was 72%. The minor difference between CT values of pools and samples can be explained by the dilution effect in the pool. However, the positive correlation between the values as well as the amount of cost saving demonstrate that pooling on nasopharyngeal samples for COVID-19 PCR testing can be a good method for efficient screening with significant resource saving. One of the most important advantages of the proposed method is the fact that samples are pooled prior extraction, which avoids the possibilities with misinterpretation of IC due to low yield of RNA in the extraction process.展开更多
In the present study, an aero pneumatic fatigue testing machine for complete dentures was designed, fabricated, and tested for the evaluation of the fatigue life of reinforced complete upper denture (CUD). On completi...In the present study, an aero pneumatic fatigue testing machine for complete dentures was designed, fabricated, and tested for the evaluation of the fatigue life of reinforced complete upper denture (CUD). On completion and testing, it was observed that the machine has the potential of generating reliable number of cyclic data. The machine’s performance was evaluated using test specimens of identical CUDs that were machined in conformity with standard procedures. The fatigue machine compressed the lower dental arch over the upper denture-specimen in centric occlusion, in the same way that the two masticatory muscles pull the lower jaw over the upper jaw during chewing. The incorporation of glass fibres into the CUD using a sandwich technique quadruples the lifespan of the denture (<em>P</em> = 0.004). The low standard deviation, along with the low coefficient of variation (CV) of the group of unreinforced dentures shows the repeatability of the results and the reliability of the machine. The high standard deviation and coefficient of variation of reinforced dentures was expected, since a high variation of results is usually recorded in fibre reinforcement cases. This research confirmed the view that the crack during denture fracture initiates in the anterior palatal area and propagates to the posterior.展开更多
In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection...In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.展开更多
With the continuous development and progress of human society, the scale of light industry and heavy industry continues to expand, which provides a large number of jobs for human beings and also brings environmental p...With the continuous development and progress of human society, the scale of light industry and heavy industry continues to expand, which provides a large number of jobs for human beings and also brings environmental pollution to human beings. In order to find a better way to solve the problem of environmental pollution, various environmental monitoring laboratories have sprung up one after the other. Environmental monitoring experiments have produced a large amount of hazardous waste. If these wastes are not properly handled, they will bring great harm to the natural environment on which human beings depend. Therefore, this paper analyzes the generation of hazardous waste in environmental monitoring laboratories and puts forward relevant effective treatment measures.展开更多
In trading activities,especially exports in the food commodities sector,several documents must be made and completed by exporters,such as Certificate of Quality,Test-Certificate,and Chemical Analysis.Balai Pengujian&a...In trading activities,especially exports in the food commodities sector,several documents must be made and completed by exporters,such as Certificate of Quality,Test-Certificate,and Chemical Analysis.Balai Pengujian&Sertifikasi Mutu Barang Makassar is a government agency operating in South Celebes that tests the quality of various agricultural and plantation commodities.From year to year,demand for testing services has increased along with the number of exports.So,testing activities have changed in terms of facility layout requirements resulting in overwhelmed in carrying out the testing process.Test rooms are not connected based on the current testing flow causing the tester’s ineffective work patterns.This study aims to redesign the layout of the facilities in the testing laboratory with the Kaizen approach to support the smooth testing process.The results of this study indicate that the proposed floor area uses a 150% allowance so the calculation requires a floor area of 42.52 m^(2).After that,an analysis of the proximity of the facilities is carried out by making ARC(Activity Relationship Chart)and ARD(Activity Relationship Diagram).Based on ARD,a template is made for the layout of testing laboratory facilities.This study applies Kaizen theory with the 5S concept to obtain a neater design,namely Seiri and Seiton in the refreshment laboratory and weighing room,Seiso on testing equipment in all testing laboratory rooms,Seiketsu and Shitsuke applied to all rooms in the testing laboratory.展开更多
Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by s...Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.展开更多
An integrated approach to geomaterial characterization is advocated that combines geology, in-situ testing, fabric studies, routine index experiments and advanced laboratory testing. It is shown that advanced laborato...An integrated approach to geomaterial characterization is advocated that combines geology, in-situ testing, fabric studies, routine index experiments and advanced laboratory testing. It is shown that advanced laboratory testing can explore features such as kinematic yielding and anisotropy in stiffness or shear strength that would otherwise be impossible to quantify. A detailed study performed in London clay at the new Heathrow Terminal 5 site is used to illustrate the arguments made. It is shown that the London clay has strong anisotropy in stiffness, is highly non-linear over the strain range of engineering interest, has markedly anisotropic shear strength characteristics and exhibits a pronounced degree of brittleness. These features can impact significantly on the practical design and analysis of civil engineering works including shallow and deep foundations, tunnels and excavations, and the stability of slopes.展开更多
基金the European Commission for its financial contribution to the LIFE20 ENV/IT/000181"SNEAK"(optimised Sur-faces against NoisE And ibrations produced by tramway tracK and road traffic).
文摘Knowledge of the dynamic modulus of bituminous mixtures is practical and theoretically meaningful in pavement design,construction,and monitoring.The tests in the laboratory for the determination of asphalt concrete(AC)moduli include the resilient modulus through the indirect tensile test(EN 12697-26),the complex modulus through the four point bending beam(EN 12697-26),the asphalt mixture performance tester(AMPT)and the simple performance tester(SPT)(AASHTO T342).Unfortunately,the tests above are time-consuming and quite expensive.On the other hand,the standard ASTM E1876 for resonant tests applies only to very thin(stocky)cylindrical samples(with a thickness-to-radius ratio,t/r,lower than 0.5)while the typical AC samples produced in the laboratory do not satisfy the ASTM E1876 requirements.Consequently,the main objective of this study is to set up and implement a tentative method to extend the range of applicability of the standard ASTM E1876 to common AC samples.The methodology was to carry out resonant tests on slender samples and to cut each of them into stocky samples(these latter complying with ASTM E1876 requirements in terms of t/r),deriving the master curve per material.These master curves allowed for deriving the value of the dynamic modulus for the given selected sample under its particular test conditions during the resonant test(i.e.,temperature and frequency).Consequently,simplified formulae were provided for AC samples.Results were compared to Witczak's estimates.These formulae provide an approximate tool to carry out low-cost and high-speed inferences at the laboratory stage on common AC samples,whatever their thickness is.Additional studies are needed to investigate the reliability of the method further and reduce uncertainties.
文摘Acute respiratory tract infection</span><span style="font-family:""><span style="font-family:Verdana;"> (ARTI) in children is the most common infectious disease in childhood, and its pathogens include viruses, bacteria and fungi, mycoplasma, chlamydia and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more clinical attention. The clinical diagnosis and treatment characteristics of acute respira</span><span style="font-family:Verdana;">tory infections in children and the research of clinical laboratory detection </span><span style="font-family:Verdana;">methods have also been continuously developed. The author collected refer</span><span style="font-family:Verdana;">ences to review the clinical features and new developments in laboratory</span><span style="font-family:Verdana;"> testing of acute respiratory tract infection in children.
文摘Acute respiratory infection in children (ARTI) is the most common childhood infectious disease, and its pathogens include bacteria, fungi, viruses, chlamydia, mycoplasma and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more attention from clinicians. The clinical diagnosis and treatment of acute respiratory infections in children and the research of laboratory detection methods have also been continuously developed. The manuscript presents a review of progress in the clinical diagnosis, treatment and laboratory testing of acute respiratory infections in children by collecting references.
文摘Objective: to discuss the related factors affecting the quality control of pharmaceutical microorganism testing laboratories and their countermeasures. Methods: the computer was used to collect the occurrence of detection bias samples in the pharmaceutical microbiology laboratory of our hospital. The retrieval period was set as June 2019 to August 2020. The causes of the bias samples were sorted out and analyzed, and the corresponding measures were formulated. Results: after investigation and statistics, 109 samples of testing deviation occurred in our hospital's pharmaceutical microorganism testing laboratory, among which the non-compliance of personnel's operation was the main reason, accounting for 34.86%. The second was the testing deviation caused by equipment problems, testing methods and standard problems, accounting for 26.61% and 14.68% respectively. Conclusion: the factors affecting the quality control management of pharmaceutical microorganism testing laboratories involve various aspects, and the laboratories should pay attention to them and formulate specific measures to prevent specific problems in order to reduce the occurrence of testing deviation events and improve the quality control management of laboratories.
基金Supported by Natural Science Foundation of Shandong Province(ZR2018PC010)Teaching and Research Project of Binzhou University(BZXYSYXM201810).
文摘In view of the difficulties about school-enterprise cooperation on food safety testing laboratory reform,the current training program,teaching methods and teaching forms are not suitable for the new platform.This paper provides new ideas and modes to solve these problems,such as integrating the resource elements between school and enterprise,establishing new platforms with the help of external force and innovating the cooperation modes to improve the laboratory,which can fully serve teaching,scientific research and enterprise production.
文摘The capacity of ground support components which have been affected by corrosion is reduced and may ultimately lead to dynamic failure of the component and the strata. In order to maintain an effective,long-term ground support system, significant campaigns of rehabilitation are often required in corrosion affected areas which also expose the workers to hazardous conditions. The most common corrosion protection for steel ground support utilises sacrificial systems such as galvanising. Galvanising has previously been proven to be susceptible to some corrosion processes. Stainless steel is the most effective in resistance to corrosion, but can be cost prohibitive, and its mechanical properties often make it unsuited to use in ground support components. Providing an outer protective plastic coating to bolts has proven to be an effective means of protecting the inner steel bar from corrosion. However, these support systems tend to be susceptible to coating damage, and require post cement grouting to provide full encapsulation. In comparison to a standard bolt/resin system, they can be slow to install and expensive.These systems have also been shown to reduce overall load transfer performance of the bolting system. In order to provide a higher level of corrosion protection whilst maintaining current installation practices and bolting cycle times, Minova has developed the Enduro^(TM)steel ground support range. The Enduro^(TM) range consists of standard Minova steel ground support components which have been treated with a unique coating process. The Enduro^(TM)coating has been tested in the harshest of conditions, in laboratory controlled conditions and in underground trials. It has been proven to effectively resist or completely eliminate the formation of corrosion, even in the most aggressive environments. This paper explains the process and provides the details of the laboratory and underground corrosion performance testing carried out on Enduro^(TM)ground support products.
基金MOH Program on Applied Research in the Prevention and Treatment of AIDS (WA 2003-17)
文摘This paper describes the development and challenge of HIV/AIDS testing laboratory network and quality assurance system in China. At present,the HIV/AIDS testing laboratories includes three classes,the National AIDS Reference Laboratory,HIV/AIDS confirmatory laboratories and HIV/AIDS screening laboratories. All of them are accredited by the health authorities,and each class of laboratories take charge of their function strictly according to the "National Management of HIV/AIDS Detection (2006)". A complete quality assurance and quality control system for HIV/AIDS testing has been developed,which includes technical training,strict laboratory monitoring and approval,examination or proficiency testing on HIV/AIDS detection,and quality evaluation and supervision of HIV/AIDS diagnostic kits. Besides conduct the routine anti-HIV antibody test,more and more laboratories began to conduct other tests,such as CD4+ T lymphocyte cell counting,HIV viral load,HIV DNA PCR,genotyping,drug resistance,and HIV-1 recent infection test. The primary challenges faced by the HIV/AIDS testing laboratory network are in the areas of laboratory management and quality control. For example,the provincial PT program is inefficient,the internal quality control is conducted perfunctorily,personnel training can not met the needs of the workplace,which need to be improved.
基金Supported by the Dongguk University Research Program(2021).
文摘BACKGROUND Isotretinoin is a widely prescribed drug for various dermatological conditions,especially acne.Blood tests are routinely performed to monitor its side effects.However,the optimal testing schedule remains controversial.AIM To evaluate the laboratory monitoring tests and schedules of patients with acne taking isotretinoin to determine the most efficient testing regimen.METHODS We retrospectively reviewed electronic medical records from the Dermatology Department of Dongguk University Ilsan Hospital from 2005 to 2020 for patients prescribed isotretinoin for acne who underwent monthly blood tests.RESULTS Aspartate aminotransferase(AST),alanine aminotransferase(ALT),and trigly-ceride(TG)levels significantly changed between 5 and 6 mo when the total prescription period and dose variables were considered altogether.The high-density lipoprotein level also significantly changed between 5 and 6 mo.However,low-density lipoprotein(LDL)and total cholesterol levels significantly changed between 1 and 2 mo.CONCLUSION We recommend testing AST,ALT,and TG levels once every 5 to 6 mo.We also suggest testing LDL and total cholesterol levels during the first and second months.
基金sponsored by the Department of Earthquake Monitoring and Prediction,China Earthquake Administration
文摘According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.
文摘The main objective of this study is to develop a new protocol for bench-scale dispersant effectiveness testing adapted for subsea dispersants injection (SSDI). The new approach includes turbulence conditions, dispersant injection techniques and quantification of effectiveness, more representative for a SSDI operation. Results from the new system are compared to dispersant effectiveness measured with established laboratory methods, used for screening dispersants for surface application. The most significant result is that the dispersant ranking obtained with the new test relevant for subsea releases was very different compared to the ranking obtained by screening methods used for surface application of dispersants. This strongly indicates that existing standard methods for dispersant effectiveness testing designed for simulating conditions relevant for surface application of dispersants, are less relevant for SSDI effectiveness testing and justify the need for a more relevant method. Such a Dispersant Injection Effectiveness Test (DIET) is suggested and documented in this study.
文摘Background: Routine pre-operative test, results in significant cost to the hospital and expense for the patient. The cost of a single laboratory test may be negligible but too many tests have increased cost for many national health schemes. Most of these patients cannot afford this extra cost thereby delaying surgery and adding up to emergencies. Implementation of change is necessary to reduce economic burden on the patient and enhance patient care. The aim of this study was to determine the economic burden of preoperative laboratory tests on pre-surgical patients in Komfo Anokye Teaching Hospital with a view to making appropriate recommendations that will reduce costs for the patients. Methodology: This was a prospective, cross-sectional study of patients undergoing elective surgery at K.A.T.H from 1st to 31st March 2014. A quantitative technique was used to effectively quantify laboratory results that were contained in a patient’s folder before an elective surgical procedure. Close and open ended questionnaire was developed and answered by reviewing patient’s folders during the pre-anaesthesia assessment. Cost of various laboratory investigations was obtained on request from one private laboratory and the KATH main laboratory. Data were analyzed using Statistical Package of Social Sciences (SSPS) version 22 and Microsoft Excel 2010. Results: The total cost of preoperative laboratory investigations in the study population was GHC 15, 321.19 (GHC 92.85 per individual);and for tests that were not indicated, GHC 3, 266.83 (GHC19.80 per individual). Conclusion: Routine preoperative laboratory tests ordered by Surgeons in Komfo Anokye Teaching Hospital impose an economic burden on the patients. The development of a comprehensive guideline that will help in the ordering of preoperative laboratory investigation will reduce costs for the patients.
基金Supported by Technical Research Project of the Ministry of Public Security "Study on DNA Characteristics of Dog Breeds"(2016JSYJA09)
文摘DNA contamination will directly result in wrong conclusion of DNA test. The higher the detection accuracy is, the greater the harmful of contamination is. DNA contamination can be divided into sample contamination and PCR contamination. It is the most difficult to treat and prevent aerosol contamination of PCR. In order to prevent pollution, dog DNA testing laboratory should be divided into several independent areas, and the researchers should strictly obey the operation rules. In addition, we should design control experiment scientifically to track pollution sources and eliminate the contamination through technical means, once the pollution occurs.
文摘Background: Request for preoperative laboratory investigations is usually done by surgeons. On some occasions, the patient may come with laboratory investigations that have been requested by the primary physician. This occurs in situations where the primary physician saw the patient first and referred to the surgeon. There is usually no indications based on history or physical examination before these laboratory request is done but rather on speculations that the “anaesthetist may require them”. This is done in order to avoid cancellation or delay of cases. The aim of this study was to find out how tests ordered in Komfo Anokye Teaching Hospital (K.A.T.H.) by the surgeons affected the decisions of the anaesthetists in the perioperative management of the patients. Methodology: This was a prospective, cross sectional study of patients undergoing elective surgery at K.A.T.H from 1st to 31st March 2014. A quantitative technique was used to effectively quantify laboratory results that were contained in a patient’s folder before an elective surgical procedure. A close and open-ended questionnaire was developed and answered by reviewing patient’s folders during the pre-anesthesia assessment. Data were analyzed using Statistical Package of Social Sciences (SSPS) version 22. Results: The average age of patients studied was 50 years. Abnormal test results did not influence the anaesthetic management in 70.5% of cases but led to either delay or cancelation of cases or requirement for transfusion of blood or blood products in 29.5% of cases. Conclusion: Preoperative laboratory tests ordered by Surgeons in KomfoAnokye Teaching Hospital do not significantly influence the anaesthetic managements of patients.
文摘Since the beginning of COVID-19 pandemics many countries were facing challenges with testing capacity recourse limitations. Throughout the waves of the pandemic countries were trying to address the existing constrains exploring solutions to increase the testing capacity with more cost-effective approaches. Pooled methodology was one of the methods which many have validated and used. It is evident that in case of pooled sample testing the sensitivity becomes lower, however the variation highly depends on the pool size as well as the incidence rate at the certain point. Armenia as well as many other countries has adopted regulations for mandatory COVID-19 PCR testing for all the travelers. Current study aimed to explore the efficiency of COVID-19 pooled PCR testing for nasopharyngeal swabs of individuals with no symptoms in a time period with good epidemiological state of the infection. Nasopharingeal swab samples from individuals were collected. The manual extraction of RNAs of samples was performed after pooling up to 5 samples. The pools with Cycle Threshold (CT) of < 37 were considered positive and were retested individually. In total 28,015 samples were grouped in 667 pools of which 57 were positive. The total number of positive samples was 65. The median difference (CT-pool–CT samples) was 2.4 (ranging from–3.0 to 8.9). The correlation of CT of pools and positive samples was positive. The correlation coefficient r = 0.84, P < 0.000, 95% CI range 0.7423 to 0.9243). The total economic saving when using pools compared to the individual testing was 72%. The minor difference between CT values of pools and samples can be explained by the dilution effect in the pool. However, the positive correlation between the values as well as the amount of cost saving demonstrate that pooling on nasopharyngeal samples for COVID-19 PCR testing can be a good method for efficient screening with significant resource saving. One of the most important advantages of the proposed method is the fact that samples are pooled prior extraction, which avoids the possibilities with misinterpretation of IC due to low yield of RNA in the extraction process.
文摘In the present study, an aero pneumatic fatigue testing machine for complete dentures was designed, fabricated, and tested for the evaluation of the fatigue life of reinforced complete upper denture (CUD). On completion and testing, it was observed that the machine has the potential of generating reliable number of cyclic data. The machine’s performance was evaluated using test specimens of identical CUDs that were machined in conformity with standard procedures. The fatigue machine compressed the lower dental arch over the upper denture-specimen in centric occlusion, in the same way that the two masticatory muscles pull the lower jaw over the upper jaw during chewing. The incorporation of glass fibres into the CUD using a sandwich technique quadruples the lifespan of the denture (<em>P</em> = 0.004). The low standard deviation, along with the low coefficient of variation (CV) of the group of unreinforced dentures shows the repeatability of the results and the reliability of the machine. The high standard deviation and coefficient of variation of reinforced dentures was expected, since a high variation of results is usually recorded in fibre reinforcement cases. This research confirmed the view that the crack during denture fracture initiates in the anterior palatal area and propagates to the posterior.
文摘In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.
文摘With the continuous development and progress of human society, the scale of light industry and heavy industry continues to expand, which provides a large number of jobs for human beings and also brings environmental pollution to human beings. In order to find a better way to solve the problem of environmental pollution, various environmental monitoring laboratories have sprung up one after the other. Environmental monitoring experiments have produced a large amount of hazardous waste. If these wastes are not properly handled, they will bring great harm to the natural environment on which human beings depend. Therefore, this paper analyzes the generation of hazardous waste in environmental monitoring laboratories and puts forward relevant effective treatment measures.
文摘In trading activities,especially exports in the food commodities sector,several documents must be made and completed by exporters,such as Certificate of Quality,Test-Certificate,and Chemical Analysis.Balai Pengujian&Sertifikasi Mutu Barang Makassar is a government agency operating in South Celebes that tests the quality of various agricultural and plantation commodities.From year to year,demand for testing services has increased along with the number of exports.So,testing activities have changed in terms of facility layout requirements resulting in overwhelmed in carrying out the testing process.Test rooms are not connected based on the current testing flow causing the tester’s ineffective work patterns.This study aims to redesign the layout of the facilities in the testing laboratory with the Kaizen approach to support the smooth testing process.The results of this study indicate that the proposed floor area uses a 150% allowance so the calculation requires a floor area of 42.52 m^(2).After that,an analysis of the proximity of the facilities is carried out by making ARC(Activity Relationship Chart)and ARD(Activity Relationship Diagram).Based on ARD,a template is made for the layout of testing laboratory facilities.This study applies Kaizen theory with the 5S concept to obtain a neater design,namely Seiri and Seiton in the refreshment laboratory and weighing room,Seiso on testing equipment in all testing laboratory rooms,Seiketsu and Shitsuke applied to all rooms in the testing laboratory.
基金supported by the National Transgenic Major Program of China(No.2019ZX08010-002)the China Agriculture Research System(No.CARS-21)the Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province,China(No.2016C02058)。
文摘Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.
基金Project supported by the UK Engineering and Physical Sciences Research Council (EPSRC)the British Airports Authority (BAA)the London Underground Limited (LUL),UK
文摘An integrated approach to geomaterial characterization is advocated that combines geology, in-situ testing, fabric studies, routine index experiments and advanced laboratory testing. It is shown that advanced laboratory testing can explore features such as kinematic yielding and anisotropy in stiffness or shear strength that would otherwise be impossible to quantify. A detailed study performed in London clay at the new Heathrow Terminal 5 site is used to illustrate the arguments made. It is shown that the London clay has strong anisotropy in stiffness, is highly non-linear over the strain range of engineering interest, has markedly anisotropic shear strength characteristics and exhibits a pronounced degree of brittleness. These features can impact significantly on the practical design and analysis of civil engineering works including shallow and deep foundations, tunnels and excavations, and the stability of slopes.
