Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and hom...Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and homologous recombination(HR)being the two most prominent.Although two major pathways have been extensively studied in Arabidopsis,rice and other mammals,the exact functions and differences between the two DSB repair pathways in maize still remain less well understood.Here,we characterized mre11a and rad50,mutants of HR pathway patterns,which showed drastic degradation of the typically persistent embryo and endosperm during kernel development.Loss of MRE11 or RAD50 function led to chromosomal fragments and chromosomal bridges in anaphase.While we also reported that the NHEJ pathway patterns,KU70 and KU80 are associated with developmental growth and genome stability.ku70 and ku80 both displayed an obvious dwarf phenotype.Cytological analysis of the mutants revealed extensive chromosome fragmentation in metaphase and subsequent stages.Loss of KU70/80 function upregulated the expression of genes involved in cell cycle progression and nuclear division.These results provide insights into how NHEJ and HR are mechanistically executed during different plant developmental periods and highlight a competitive and complementary relationship between the NHEJ and HR pathways for DNA double-strand break repair in maize.展开更多
Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/...Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/dissimilar RFSSW joint exhibits remarkable and endurable characteristics,including high shear strength,long fatigue life,and strong corrosion resistance.In the meanwhile,as the key-hole free joint has different microstructures compared with conventional friction stir spot welding,thus the RFSSW joint shall possess different shear and fatigue fracture mechanisms,which needs further investigation.To explore the underlying failure mechanism,the similar/dissimilar metallic material joining parameters and pre-treatment,mechanical properties,as well as fracture mechanisms under this novel technology will be discussed.In details,the welding tool design,welding parameters setting,and the influence of processing on the lap shear and fatigue properties,as well as the corrosion resistance will be mainly discussed.Moreover,the roadmap of RFFSW is also discussed.展开更多
Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeu...Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA repair.Among the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential role.In this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in vivo.Conversely,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP treatment.Mechanistically,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of CDK1.Meanwhile,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy drugs.Our transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant cells.In summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.展开更多
Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRP...Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRPs)through laser-assisted metal and plastic direct joining(LAMP).This study uses the LAMP technique to produce AZ31-CFRP joints.The joining process involves as-received AZ31,HFpretreated AZ31,and thermally oxidized HF-pretreated AZ31 alloy sheets.Furthermore,the bonding strength of joints prepared with thermally oxidized AZ31 alloy sheets is examined to ascertain the combined effect of HF treatment and thermal oxidation on bonding strength.The microstructures,surface chemical interactions,and mechanical performances of joints are investigated under tensile shear loading.Various factors,such as bubble formation,CFRP resin decomposition,and mechanical interlocking considerably affect joint strength.Additionally,surface chemical interactions between the active species on metal parts and the polar amide along with carbonyl groups of polymer play a significant role in improving joint strength.Joints prepared with surface-pretreated AZ31 alloy sheets show significant improvements in bonding strength.展开更多
Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward high...Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward higher requirements for the application of joining technology of high-strength steel/Al dissimilar materials. Taking the new die-casting Al alloy body as an example, this paper systematically studies the progress of the latest joining methods of steel/Al dissimilar material with combination of two-layer plate and three-layer plate. By analyzing the joining technologies such as FSPR, RES, FDS and SPR, the technology and process characteristics of steel/Al dissimilar material joining are studied, and the joining technical feasibility and realization means of different material combination of the body are analyzed. The conditions of material combination, material thickness, material strength, flange height, preformed holes and joint spacing for achieving high-quality joining are given. The FSPR joining technology is developed and tested in order to meet with the joining of parts with DCAA and TFSS, especially for the joining of three-layer plates with them. It finds the method and technical basis for the realization of high quality joining of dissimilar materials, provides the early conditions for the application of large DCAA and TFSS parts in body-in-white, and meets the design requirements of new energy body. .展开更多
Advanced high strength steel,aluminum alloy and plastic materials are used in the right places for the purpose of reducing the weight of EV(electric vehicle)bodies and in-vehicle parts,and multi-material structures ar...Advanced high strength steel,aluminum alloy and plastic materials are used in the right places for the purpose of reducing the weight of EV(electric vehicle)bodies and in-vehicle parts,and multi-material structures are advancing.Therefore,it is difficult to handle the welding and joining processes of automobile structures by the conventional arc welding and resistance spot welding,which have been applied to steel joining,and various joining processes are being applied depending on the material.Under above mentioned background,the authors have developed some unique joining processes for multi-materials that are used in the right place.This paper introduces the dissimilar metal joining between the galvanized steel and aluminum alloy by laser arc hybrid process,the metal/thermoplastic dissimilar material joining using laser process and the solid-state resistance spot joining process of advanced high strength steel for EV body structural parts.Moreover,the authors describe the high-speed plasma jet GTA(Gas Tungusten Arc)welding process of copper applied to electrical components such as motors.展开更多
The research and development of joining methods of ceramics to metals, especially brazing, diffusion bonding and partial transition liquid phase bonding, were introduced. Some opinions were put forward. For new compos...The research and development of joining methods of ceramics to metals, especially brazing, diffusion bonding and partial transition liquid phase bonding, were introduced. Some opinions were put forward. For new composites emerging, it is necessary to develop new joining methods, particularly in the field of high temperature technique for joining ceramics to superalloys.展开更多
Grid pattern was textured on Ti-6 Al-4 V alloy(TC4)substrate surface by nanosecond laser system.Laser joining of carbon fiber reinforced thermoplastic composite(CFRTP)to TC4 joints were performed,and the effect of tex...Grid pattern was textured on Ti-6 Al-4 V alloy(TC4)substrate surface by nanosecond laser system.Laser joining of carbon fiber reinforced thermoplastic composite(CFRTP)to TC4 joints were performed,and the effect of texture grid depth was investigated.The contact angle of molten CFRTP on textured TC4 surface was measured and the tensile-shear force was tested.The fracture surface and interface morphology were observed.The results indicated that the wettability of molten CFRTP on TC4 surface improved remarkably after laser textured TC4.Shear force of CFRTP/TC4 joints was increased by 156%after laser textured TC4 surface.When the depth of grid was deeper than 100μm,contact angle increased and incomplete filling of molten CFRTP in grid occurred,the shear force thus decreased gradually.Resin-carbon fibers mixture was adhered on the fracture surface of TC4,and the variation tendency of adhesion ratio was consistent with that of shear force.TC4 matrix was exfoliated from substrate and adhered at the fracture surface of CFRTP,indicating stronger mechanical interlocking occurred at the joining interface after laser textured TC4 surface.Beside mechanical interlocking,compound layer consisted of CTi_(0.42)V_(1.58)carburization phase was also confirmed at interface,suggesting that chemical bonding also occurred at the joining interface.展开更多
A method to reach the globular weld structure of A356 aluminum alloy using stirring the localized semisolid zone during butt-joining is developed.Since the heat conductivity of this alloy is very high, the accurate co...A method to reach the globular weld structure of A356 aluminum alloy using stirring the localized semisolid zone during butt-joining is developed.Since the heat conductivity of this alloy is very high, the accurate controlling of temperature during joining must be considered.A gas heating system was used to heat up the nitrogen gas up to the required temperature.A dried and free oxygen gas was prepared when a stream of nitrogen gas could pass closely around a hot element.Hot and pure nitrogen gas flow through a precise ceramic nozzle was used to create a localized semisolid pool.At this stage a fine stirrer was introduced into the weld seam in order to mix the two sides into a single uniform joint.Substrates were moved in direction of joint line by a small trolley to avoid the deviation of nozzle from the joint line and its distance and angle from the substrate.A fixture system was used to hold two substrates together on the trolley.A narrow hot plate was located on the trolley to heat up the joint line due to high heat conductivity of aluminum.Effect of gas temperature was investigated on the microstructure and mechanical properties of weld seam.Results showed that increase in temperature promoted the final welding properties, and also at liquid fractions less than 50% joining was not fully practical.The best mechanical properties were achieved with liquid fraction of about 70%.展开更多
Electricity is an efficient form of energy,and the growing interest in electricity-assisted manufacturing is motivated by its inherent energy saving and reduced environmental impact.Beyond this,Electromagnetic Process...Electricity is an efficient form of energy,and the growing interest in electricity-assisted manufacturing is motivated by its inherent energy saving and reduced environmental impact.Beyond this,Electromagnetic Processing of Materials(EPM)allows the fabrication of materials with new compositions,metastable phases and nanograined microstructures that cannot be obtained using conventional heating processes using furnaces.This review covers EPM for the manufacture of ceramic and metal bulk components,with a specific focus on the effects of electric fields and electromagnetic radiations on processing in a wide spectrum of frequencies ranging from DC(f=0 Hz)to visible light(f=10^(14)–10^(15)Hz).The manuscript is divided into two parts.The first part provides a comprehensive overview of the interactions between matter and electric field/current,including heating phenomena(resistive Joule,induction,dielectric heating,electric arcs)and athermal effects(electromigration,electroplasticity,electrochemical reactions,ponderomotive force and others).The second part is focused on the technological application of the techniques,covering heat treatments,joining,sintering and forming.Seven distinct physical phenomena are involved in EPM:resistive Joule and induction heating,electrochemical reactions,electroplasticity,electric arcs and electromagnetic heating based on radio and microwave frequencies(mainly used for heating dielectric materials;i.e.,dielectric heating)or on the IR/visible light(IR heating and lasers).展开更多
SiCp/Al composites have excellent comprehensive properties and have been widely used in aerospace,automotive industry and other fields.Due to the huge difference in performance between SiC particles and matrix alloys,...SiCp/Al composites have excellent comprehensive properties and have been widely used in aerospace,automotive industry and other fields.Due to the huge difference in performance between SiC particles and matrix alloys,traditional fusion welding methods are difficult to meet the join requirements of SiCp/Al composites.Friction stir joining(friction stir welding),as a solid phase joining process,has been proved to be a new technology with fine prospect in joining SiCp/Al composites compared with fusion welding process.Although some progress has been made in recent years,there are still full of challenges.In this paper,the research status of friction stir joining of SiCp/Al composites in recent years is expatiated,including the weldability of SiCp/Al composites,the macrostructure and the microstructure of joints,mechanical properties of joints,and tool wear and monitoring.Furthermore,the existing challenges of friction stir joining of SiCp/Al composites are summarized and the future development directions are prospected.展开更多
Vaporizing foil actuator welding(VFAW) was used for joining 2024-T3 and 7075-T6 aluminum alloy sheets, and the resulting joint microstructure was analyzed. 2024/7075 aluminum alloy pairs with suitable processing param...Vaporizing foil actuator welding(VFAW) was used for joining 2024-T3 and 7075-T6 aluminum alloy sheets, and the resulting joint microstructure was analyzed. 2024/7075 aluminum alloy pairs with suitable processing parameters can be prepared by using VFAW. Dynamic preform addresses the poor formability problem of target material and advantage of VFAW on dissimilar materials in some conditions. But with standoff sheet inserting in the flyer and target, 2024/7075 welded pairs gets the better weld strength, compared with flyer preformed method. The microstructure of the circular weld area of the welded joint showed a wave interface, in which a thin melt layer formed at the center and edge parts. The crystal grains near the bonding interface were remarkably elongated and refined. Therefore, the joining of the 2024/7075 pairs was facilitated through plastic forming and melting.展开更多
Currently,in the automotive industry,joining of the aluminum alloys with the steel is a crucial problem to be solved.Conventional joining techniques including resistance spot and gas metal arc welding are not acceptab...Currently,in the automotive industry,joining of the aluminum alloys with the steel is a crucial problem to be solved.Conventional joining techniques including resistance spot and gas metal arc welding are not acceptable for those applications due to a number of metallurgical problems.The investigation was carried out to develop the hybrid joining process combining the resistance spot welding and brazing.In this study,an attempt was made to apply hybrid process to the joining of dissimilar sheet metals,Al-Mg-Si（6000 series） alloy and low carbon steel sheet.Hybrid process（resistance spot weld/brazing） using filler metal was found to be effective to overcome the incompatibility between aluminum alloy and steel.Although hybrid joining process of Al alloy sheet and steel sheet did not produce acceptable bond strength,it was proved to have reasonable interfacial bond layer if the optimal process condition was applied.展开更多
Dynamical Joining of the solid-state metal is the key technology to realize endless hot rolling. The heating and laser welding method both require long joining time. Based on super deformation method, a 7-bar and 2-sl...Dynamical Joining of the solid-state metal is the key technology to realize endless hot rolling. The heating and laser welding method both require long joining time. Based on super deformation method, a 7-bar and 2-slider mechanism was developed in Japan, and the joining time is less than 0.5 s, however the length of each bar are not reported and this mechanism is complex. A relatively simple 6-bar and 1-slider mechanism is put forward, which can realize the shearing and extrusion motion of the top and bottom blades with a speed approximately equal to the speed of the metal plates. In order to study the kinematics property of the double blades, based on complex vector method, the multi-rigid-body model is built, and the displacement and speed functions of the double blades, the joining time and joining thickness are deduced, the kinematics analysis shows that the initial parameters can＇t satisfy the joining process. Hence, optimization of this mechanism is employed using genetic algorithm（GA） and the optimization parameters of this mechanism are obtained, the kinematics analysis show that the joining time is less than 0.1 s, the joining thickness is more than 80% of the thickness of the solid-state metal, and the horizontal speeds of the blades are improved. A new mechanism is provided for the joining of the solid-state metal and a foundation is laid for the design of the device.展开更多
Electron beam surfi-sculpt is a new metal surface processing technology,which is widely used in the areas of materials surface treatment,composite materials manufacturing,and so on.In this paper,comeld technology,whic...Electron beam surfi-sculpt is a new metal surface processing technology,which is widely used in the areas of materials surface treatment,composite materials manufacturing,and so on.In this paper,comeld technology,which was an application technology of electron beam surfi-sculpt during composite materials area,was introduced.And tensile experiments results and failure forms of Ti6Al4V/Carbon fibre single step joints were compared with different joining methods,such as slick embedding,adhesive bonding,riveting and comeld.It was found that comeld joint had more advantage of tensile property than other joining methods.At the same time,tensile process of comeld joint was analyzed with numerical simulation,and key factors of protrusions influenced tensile property were obtained,which was of importance to structure design and joint property improvement.展开更多
Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the sit...Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the site of damage, resulting in the loss of information there. NHEJ does not restore the lost information and may resect additional nucleotides during the repair process. The ability to repair a wide range of overhang and damage configurations reflects the flexibility of the nuclease, polymerases, and ligase of NHEJ. The flexibility of the individual components also explains the large number of ways in which NHEJ can repair any given pair of DNA ends. The loss of information locally at sites of NHEJ repair may contribute to cancer and aging, but the action by NHEJ ensures that entire segments of chromosomes are not lost.展开更多
The joining of metal and polymer is an increasingly important method to get lightweight components in the development of manufacturing industry- nowadays. In this artiele, metal and polymer lap joint was achieved by m...The joining of metal and polymer is an increasingly important method to get lightweight components in the development of manufacturing industry- nowadays. In this artiele, metal and polymer lap joint was achieved by means of resistance spot welding （RSW） and ultrasonic assistance welding （UAW）. The joining mechanism of lap joint was analyzed by OM, TEM on microstructure at the interface of lap joints and XPS and IR spectra was discussed based on the following different ones： mechanical-interlocking, diffusion bond and coordination bond. The results showed that it was the combined action that played an important role in the effective joining work. Besides, ultrasonic assistance was used in the study to aid welding process based on its high-frequency ultrasonic vibration, which made joints shaping better and improved tensile strength visibly contrast to joints with the same lower heat input parameters.展开更多
The aim of this study was to develop a high-efficiency joining method of Cf/Al composites and TiA l alloys under the heat effect of laser-ignited self-propagating high-temperature synthesis（SHS）. The SHS reaction of...The aim of this study was to develop a high-efficiency joining method of Cf/Al composites and TiA l alloys under the heat effect of laser-ignited self-propagating high-temperature synthesis（SHS）. The SHS reaction of Ni–Al–Zr interlayer was induced by laser beam and acted as local high-temperature heat source during the joining. Sound joint was obtained and verified the feasibility of this joining method. Effect of filler metals on the joint microstructure and shear strength was evaluated. When the joining pressure was 2 MPa with additive filler metals, joint shear strength reached the maximum of 41.01 MPa.展开更多
In present work the weldings of an austenitic stainless steel （AISI 304L） and a ferritic carbon steel （St37） were conducted by tungsten inert gas （TIG） welding process using four different austenitic filler meta...In present work the weldings of an austenitic stainless steel （AISI 304L） and a ferritic carbon steel （St37） were conducted by tungsten inert gas （TIG） welding process using four different austenitic filler metals, namely ER308L, ER309L, ER316L and ER310. Microstructure characteristics and mechanical properties of the weldments were studied using optical and scanning electron microscopy, ferrit-ometry, hardness, tensile and impact tests. The ferrite number （_N-~） of the weldments made by different electrodes varies between 0.5 and 9.5. It was found that the increase in amount of delta ferrite in the microstructure of the weld metals, causes the decrease of the impact toughness of the weldments. It seems that using ER309L and ER316L electrodes can provide a good combination between the mechanical and metallurgical properties of the joint in AISI 304L/St37 dissimilar welding.展开更多
In this study, it was reported a novel approach for joining Cf/A1 composites and TiA1 intermetallic by self- propagating high-temperature synthesis （SHS）. Mixed powders of 14A1-2Ni-3CuO were used as the SHS inter- l...In this study, it was reported a novel approach for joining Cf/A1 composites and TiA1 intermetallic by self- propagating high-temperature synthesis （SHS）. Mixed powders of 14A1-2Ni-3CuO were used as the SHS inter- layer, and differential thermal analysis test of A1-Ni-CuO interlayer was conducted to analyze the exothermic char- acteristic. Sound joint was got by SHS joining under the conditions of 600 ℃, 30 min, and 5 MPa. The joint was characterized by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS）, and X-ray diffrac- tion （XRD）. TiA13 and NiA13 are, respectively, formed in the TiA1/interlayer and Cf/A1/interlayer interfaces. Reac- tion products of Ni2Al3, NiAl3, A1203, and Cu were observed in the interlayer. And the formation mechanism of SHS joining was investigated.展开更多
基金supported by the National Natural Science Foundation of China(32372116)to Yan He.
文摘Chromosomal DNA double-strand breaks(DSBs)are often generated in the genome of all living organisms.To combat DNA damage,organisms have evolved several DSB repair mechanisms,with nonhomologous end-joining(NHEJ)and homologous recombination(HR)being the two most prominent.Although two major pathways have been extensively studied in Arabidopsis,rice and other mammals,the exact functions and differences between the two DSB repair pathways in maize still remain less well understood.Here,we characterized mre11a and rad50,mutants of HR pathway patterns,which showed drastic degradation of the typically persistent embryo and endosperm during kernel development.Loss of MRE11 or RAD50 function led to chromosomal fragments and chromosomal bridges in anaphase.While we also reported that the NHEJ pathway patterns,KU70 and KU80 are associated with developmental growth and genome stability.ku70 and ku80 both displayed an obvious dwarf phenotype.Cytological analysis of the mutants revealed extensive chromosome fragmentation in metaphase and subsequent stages.Loss of KU70/80 function upregulated the expression of genes involved in cell cycle progression and nuclear division.These results provide insights into how NHEJ and HR are mechanistically executed during different plant developmental periods and highlight a competitive and complementary relationship between the NHEJ and HR pathways for DNA double-strand break repair in maize.
基金This work was supported by International Science and Technology Cooperation Project of Guangdong Province(Grant No.2022A0505050054)Innovation and Technology Fund(ITF)(Grant No.ITP/021/19AP)National Natural Science Foundation of China(Grant No.51905112).
文摘Refill friction stir spot welding(RFSSW)provides a novel method to join similar and/or dissimilar metallic materials without a key-hole in the center of the joint.Having the key-hole free characterization,the similar/dissimilar RFSSW joint exhibits remarkable and endurable characteristics,including high shear strength,long fatigue life,and strong corrosion resistance.In the meanwhile,as the key-hole free joint has different microstructures compared with conventional friction stir spot welding,thus the RFSSW joint shall possess different shear and fatigue fracture mechanisms,which needs further investigation.To explore the underlying failure mechanism,the similar/dissimilar metallic material joining parameters and pre-treatment,mechanical properties,as well as fracture mechanisms under this novel technology will be discussed.In details,the welding tool design,welding parameters setting,and the influence of processing on the lap shear and fatigue properties,as well as the corrosion resistance will be mainly discussed.Moreover,the roadmap of RFFSW is also discussed.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.:82003807,82173394)the Shaanxi Province Science Foundation,China(Grant No.:2023-GHZD-19)+1 种基金the Medical Foundation-Clinical Integration Program of Xi'an Jiaotong University,China(Grant No.:YXJLRH2022043)the Xi'an Jiaotong University Free Exploration and Innovation-Teacher Project Foundation,China(Grant No.:xzy012023104).
文摘Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA repair.Among the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential role.In this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in vivo.Conversely,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP treatment.Mechanistically,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of CDK1.Meanwhile,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy drugs.Our transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant cells.In summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.
基金supported by the Nano&Material Technology Development Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(RS-2023-00234757).
文摘Although hydrofluoric acid(HF)surface treatment is known to enhance the joining of metals with polymers,there is limited information on its effect on the joining of AZ31 alloy and carbon-fiber-reinforced plastics(CFRPs)through laser-assisted metal and plastic direct joining(LAMP).This study uses the LAMP technique to produce AZ31-CFRP joints.The joining process involves as-received AZ31,HFpretreated AZ31,and thermally oxidized HF-pretreated AZ31 alloy sheets.Furthermore,the bonding strength of joints prepared with thermally oxidized AZ31 alloy sheets is examined to ascertain the combined effect of HF treatment and thermal oxidation on bonding strength.The microstructures,surface chemical interactions,and mechanical performances of joints are investigated under tensile shear loading.Various factors,such as bubble formation,CFRP resin decomposition,and mechanical interlocking considerably affect joint strength.Additionally,surface chemical interactions between the active species on metal parts and the polar amide along with carbonyl groups of polymer play a significant role in improving joint strength.Joints prepared with surface-pretreated AZ31 alloy sheets show significant improvements in bonding strength.
文摘Green and low carbon promote the application and development of light-weight materials in body-in-white. Large-scale die-casting Al alloy (DCAA) and high-strength thermo-formed steel sheet (TFSS) have put forward higher requirements for the application of joining technology of high-strength steel/Al dissimilar materials. Taking the new die-casting Al alloy body as an example, this paper systematically studies the progress of the latest joining methods of steel/Al dissimilar material with combination of two-layer plate and three-layer plate. By analyzing the joining technologies such as FSPR, RES, FDS and SPR, the technology and process characteristics of steel/Al dissimilar material joining are studied, and the joining technical feasibility and realization means of different material combination of the body are analyzed. The conditions of material combination, material thickness, material strength, flange height, preformed holes and joint spacing for achieving high-quality joining are given. The FSPR joining technology is developed and tested in order to meet with the joining of parts with DCAA and TFSS, especially for the joining of three-layer plates with them. It finds the method and technical basis for the realization of high quality joining of dissimilar materials, provides the early conditions for the application of large DCAA and TFSS parts in body-in-white, and meets the design requirements of new energy body. .
文摘Advanced high strength steel,aluminum alloy and plastic materials are used in the right places for the purpose of reducing the weight of EV(electric vehicle)bodies and in-vehicle parts,and multi-material structures are advancing.Therefore,it is difficult to handle the welding and joining processes of automobile structures by the conventional arc welding and resistance spot welding,which have been applied to steel joining,and various joining processes are being applied depending on the material.Under above mentioned background,the authors have developed some unique joining processes for multi-materials that are used in the right place.This paper introduces the dissimilar metal joining between the galvanized steel and aluminum alloy by laser arc hybrid process,the metal/thermoplastic dissimilar material joining using laser process and the solid-state resistance spot joining process of advanced high strength steel for EV body structural parts.Moreover,the authors describe the high-speed plasma jet GTA(Gas Tungusten Arc)welding process of copper applied to electrical components such as motors.
文摘The research and development of joining methods of ceramics to metals, especially brazing, diffusion bonding and partial transition liquid phase bonding, were introduced. Some opinions were put forward. For new composites emerging, it is necessary to develop new joining methods, particularly in the field of high temperature technique for joining ceramics to superalloys.
基金financially supported by National Natural Science Foundation of China(No.51875129)。
文摘Grid pattern was textured on Ti-6 Al-4 V alloy(TC4)substrate surface by nanosecond laser system.Laser joining of carbon fiber reinforced thermoplastic composite(CFRTP)to TC4 joints were performed,and the effect of texture grid depth was investigated.The contact angle of molten CFRTP on textured TC4 surface was measured and the tensile-shear force was tested.The fracture surface and interface morphology were observed.The results indicated that the wettability of molten CFRTP on TC4 surface improved remarkably after laser textured TC4.Shear force of CFRTP/TC4 joints was increased by 156%after laser textured TC4 surface.When the depth of grid was deeper than 100μm,contact angle increased and incomplete filling of molten CFRTP in grid occurred,the shear force thus decreased gradually.Resin-carbon fibers mixture was adhered on the fracture surface of TC4,and the variation tendency of adhesion ratio was consistent with that of shear force.TC4 matrix was exfoliated from substrate and adhered at the fracture surface of CFRTP,indicating stronger mechanical interlocking occurred at the joining interface after laser textured TC4 surface.Beside mechanical interlocking,compound layer consisted of CTi_(0.42)V_(1.58)carburization phase was also confirmed at interface,suggesting that chemical bonding also occurred at the joining interface.
文摘A method to reach the globular weld structure of A356 aluminum alloy using stirring the localized semisolid zone during butt-joining is developed.Since the heat conductivity of this alloy is very high, the accurate controlling of temperature during joining must be considered.A gas heating system was used to heat up the nitrogen gas up to the required temperature.A dried and free oxygen gas was prepared when a stream of nitrogen gas could pass closely around a hot element.Hot and pure nitrogen gas flow through a precise ceramic nozzle was used to create a localized semisolid pool.At this stage a fine stirrer was introduced into the weld seam in order to mix the two sides into a single uniform joint.Substrates were moved in direction of joint line by a small trolley to avoid the deviation of nozzle from the joint line and its distance and angle from the substrate.A fixture system was used to hold two substrates together on the trolley.A narrow hot plate was located on the trolley to heat up the joint line due to high heat conductivity of aluminum.Effect of gas temperature was investigated on the microstructure and mechanical properties of weld seam.Results showed that increase in temperature promoted the final welding properties, and also at liquid fractions less than 50% joining was not fully practical.The best mechanical properties were achieved with liquid fraction of about 70%.
基金the Thousand Talents Program of China and Sichuan Province。
文摘Electricity is an efficient form of energy,and the growing interest in electricity-assisted manufacturing is motivated by its inherent energy saving and reduced environmental impact.Beyond this,Electromagnetic Processing of Materials(EPM)allows the fabrication of materials with new compositions,metastable phases and nanograined microstructures that cannot be obtained using conventional heating processes using furnaces.This review covers EPM for the manufacture of ceramic and metal bulk components,with a specific focus on the effects of electric fields and electromagnetic radiations on processing in a wide spectrum of frequencies ranging from DC(f=0 Hz)to visible light(f=10^(14)–10^(15)Hz).The manuscript is divided into two parts.The first part provides a comprehensive overview of the interactions between matter and electric field/current,including heating phenomena(resistive Joule,induction,dielectric heating,electric arcs)and athermal effects(electromigration,electroplasticity,electrochemical reactions,ponderomotive force and others).The second part is focused on the technological application of the techniques,covering heat treatments,joining,sintering and forming.Seven distinct physical phenomena are involved in EPM:resistive Joule and induction heating,electrochemical reactions,electroplasticity,electric arcs and electromagnetic heating based on radio and microwave frequencies(mainly used for heating dielectric materials;i.e.,dielectric heating)or on the IR/visible light(IR heating and lasers).
基金supported by the National Natural Science Foundation of China(No.51675270)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX17_0241)the China Postdoctoral Science Foundation(No.2016M600411)。
文摘SiCp/Al composites have excellent comprehensive properties and have been widely used in aerospace,automotive industry and other fields.Due to the huge difference in performance between SiC particles and matrix alloys,traditional fusion welding methods are difficult to meet the join requirements of SiCp/Al composites.Friction stir joining(friction stir welding),as a solid phase joining process,has been proved to be a new technology with fine prospect in joining SiCp/Al composites compared with fusion welding process.Although some progress has been made in recent years,there are still full of challenges.In this paper,the research status of friction stir joining of SiCp/Al composites in recent years is expatiated,including the weldability of SiCp/Al composites,the macrostructure and the microstructure of joints,mechanical properties of joints,and tool wear and monitoring.Furthermore,the existing challenges of friction stir joining of SiCp/Al composites are summarized and the future development directions are prospected.
基金Funded by the State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body(No.31815008)the National Natural Science Foundation of China(No.U1564202,NO.51205298)111 Project(No.B17034)
文摘Vaporizing foil actuator welding(VFAW) was used for joining 2024-T3 and 7075-T6 aluminum alloy sheets, and the resulting joint microstructure was analyzed. 2024/7075 aluminum alloy pairs with suitable processing parameters can be prepared by using VFAW. Dynamic preform addresses the poor formability problem of target material and advantage of VFAW on dissimilar materials in some conditions. But with standoff sheet inserting in the flyer and target, 2024/7075 welded pairs gets the better weld strength, compared with flyer preformed method. The microstructure of the circular weld area of the welded joint showed a wave interface, in which a thin melt layer formed at the center and edge parts. The crystal grains near the bonding interface were remarkably elongated and refined. Therefore, the joining of the 2024/7075 pairs was facilitated through plastic forming and melting.
文摘Currently,in the automotive industry,joining of the aluminum alloys with the steel is a crucial problem to be solved.Conventional joining techniques including resistance spot and gas metal arc welding are not acceptable for those applications due to a number of metallurgical problems.The investigation was carried out to develop the hybrid joining process combining the resistance spot welding and brazing.In this study,an attempt was made to apply hybrid process to the joining of dissimilar sheet metals,Al-Mg-Si（6000 series） alloy and low carbon steel sheet.Hybrid process（resistance spot weld/brazing） using filler metal was found to be effective to overcome the incompatibility between aluminum alloy and steel.Although hybrid joining process of Al alloy sheet and steel sheet did not produce acceptable bond strength,it was proved to have reasonable interfacial bond layer if the optimal process condition was applied.
基金Supported by National Natural Science Foundation of China(Grant No.51475139)
文摘Dynamical Joining of the solid-state metal is the key technology to realize endless hot rolling. The heating and laser welding method both require long joining time. Based on super deformation method, a 7-bar and 2-slider mechanism was developed in Japan, and the joining time is less than 0.5 s, however the length of each bar are not reported and this mechanism is complex. A relatively simple 6-bar and 1-slider mechanism is put forward, which can realize the shearing and extrusion motion of the top and bottom blades with a speed approximately equal to the speed of the metal plates. In order to study the kinematics property of the double blades, based on complex vector method, the multi-rigid-body model is built, and the displacement and speed functions of the double blades, the joining time and joining thickness are deduced, the kinematics analysis shows that the initial parameters can＇t satisfy the joining process. Hence, optimization of this mechanism is employed using genetic algorithm（GA） and the optimization parameters of this mechanism are obtained, the kinematics analysis show that the joining time is less than 0.1 s, the joining thickness is more than 80% of the thickness of the solid-state metal, and the horizontal speeds of the blades are improved. A new mechanism is provided for the joining of the solid-state metal and a foundation is laid for the design of the device.
基金Project50975268,supported by the National Natural Science Foundation of ChinaProject2008ZE25010,supported byAeronautical Science Foundation of China
文摘Electron beam surfi-sculpt is a new metal surface processing technology,which is widely used in the areas of materials surface treatment,composite materials manufacturing,and so on.In this paper,comeld technology,which was an application technology of electron beam surfi-sculpt during composite materials area,was introduced.And tensile experiments results and failure forms of Ti6Al4V/Carbon fibre single step joints were compared with different joining methods,such as slick embedding,adhesive bonding,riveting and comeld.It was found that comeld joint had more advantage of tensile property than other joining methods.At the same time,tensile process of comeld joint was analyzed with numerical simulation,and key factors of protrusions influenced tensile property were obtained,which was of importance to structure design and joint property improvement.
文摘Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the site of damage, resulting in the loss of information there. NHEJ does not restore the lost information and may resect additional nucleotides during the repair process. The ability to repair a wide range of overhang and damage configurations reflects the flexibility of the nuclease, polymerases, and ligase of NHEJ. The flexibility of the individual components also explains the large number of ways in which NHEJ can repair any given pair of DNA ends. The loss of information locally at sites of NHEJ repair may contribute to cancer and aging, but the action by NHEJ ensures that entire segments of chromosomes are not lost.
文摘The joining of metal and polymer is an increasingly important method to get lightweight components in the development of manufacturing industry- nowadays. In this artiele, metal and polymer lap joint was achieved by means of resistance spot welding （RSW） and ultrasonic assistance welding （UAW）. The joining mechanism of lap joint was analyzed by OM, TEM on microstructure at the interface of lap joints and XPS and IR spectra was discussed based on the following different ones： mechanical-interlocking, diffusion bond and coordination bond. The results showed that it was the combined action that played an important role in the effective joining work. Besides, ultrasonic assistance was used in the study to aid welding process based on its high-frequency ultrasonic vibration, which made joints shaping better and improved tensile strength visibly contrast to joints with the same lower heat input parameters.
基金financially supported by the National Natural Science Foundation of China(Grant No.51075101)
文摘The aim of this study was to develop a high-efficiency joining method of Cf/Al composites and TiA l alloys under the heat effect of laser-ignited self-propagating high-temperature synthesis（SHS）. The SHS reaction of Ni–Al–Zr interlayer was induced by laser beam and acted as local high-temperature heat source during the joining. Sound joint was obtained and verified the feasibility of this joining method. Effect of filler metals on the joint microstructure and shear strength was evaluated. When the joining pressure was 2 MPa with additive filler metals, joint shear strength reached the maximum of 41.01 MPa.
文摘In present work the weldings of an austenitic stainless steel （AISI 304L） and a ferritic carbon steel （St37） were conducted by tungsten inert gas （TIG） welding process using four different austenitic filler metals, namely ER308L, ER309L, ER316L and ER310. Microstructure characteristics and mechanical properties of the weldments were studied using optical and scanning electron microscopy, ferrit-ometry, hardness, tensile and impact tests. The ferrite number （_N-~） of the weldments made by different electrodes varies between 0.5 and 9.5. It was found that the increase in amount of delta ferrite in the microstructure of the weld metals, causes the decrease of the impact toughness of the weldments. It seems that using ER309L and ER316L electrodes can provide a good combination between the mechanical and metallurgical properties of the joint in AISI 304L/St37 dissimilar welding.
基金financially supported by the National Natural Science Foundation of China (No. 51075101)
文摘In this study, it was reported a novel approach for joining Cf/A1 composites and TiA1 intermetallic by self- propagating high-temperature synthesis （SHS）. Mixed powders of 14A1-2Ni-3CuO were used as the SHS inter- layer, and differential thermal analysis test of A1-Ni-CuO interlayer was conducted to analyze the exothermic char- acteristic. Sound joint was got by SHS joining under the conditions of 600 ℃, 30 min, and 5 MPa. The joint was characterized by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS）, and X-ray diffrac- tion （XRD）. TiA13 and NiA13 are, respectively, formed in the TiA1/interlayer and Cf/A1/interlayer interfaces. Reac- tion products of Ni2Al3, NiAl3, A1203, and Cu were observed in the interlayer. And the formation mechanism of SHS joining was investigated.
