Three previously undescribed compounds,cordycicadione(1),cordycicadin F(2),and 7-hydroxybassiatin(3),were isolated from the cultures of Cordyceps cicadae JXCH1,an entomopathogenic fungus.Their structures and relative ...Three previously undescribed compounds,cordycicadione(1),cordycicadin F(2),and 7-hydroxybassiatin(3),were isolated from the cultures of Cordyceps cicadae JXCH1,an entomopathogenic fungus.Their structures and relative configurations were elucidated primarily by NMR spectroscopic analysis.The absolute configurations of 1 and 2 were determined by ECD calculations.Single-crystal X-ray diffraction method was adopted to determine the absolute configuration of 3.Compound 2 is a polycyclic polyketide with an unusual enol ether moiety and a spiro ring.The compounds obtained in this study were subjected to screening their inhibition against the proliferation of the human lung cancer cell line A549 and the production of nitric oxide in murine macrophages RAW264.7.展开更多
Fungal pathogens produce secretory ribonuclease(RNase)T2 proteins during infection,which contribute to fungal virulence via their enzyme functions in degradation of host cell RNA.However,the details of those proteins ...Fungal pathogens produce secretory ribonuclease(RNase)T2 proteins during infection,which contribute to fungal virulence via their enzyme functions in degradation of host cell RNA.However,the details of those proteins entering the host cells are unclear.Our previous study demonstrated that the two secretory RNase T2 members,BbRNT2 and BbTrv,produced by the insect fungal pathogen Beauveria bassiana,caused cytotoxic damage to insect cells and contributed to fungal virulence.Here,the Spodoptera frugiperda ovarian epithelial cells(sf9 cells)were used as models to investigate the interactions of the two fungus-produced RNase T2 proteins with the insect cells.Two transmembrane proteins,an ABC transporter(SfABCG)and an Innexin 7-like protein(Sfinx),were identified from the sf9 cells as interacting with BbRNT2 and BbTrv,respectively,through protein immunoprecipitation,yeast-two hybrid tests and protein pull-down assays.Although a slight decrease in the sf9 cell viability was examined by transfection of RNA interference of SfABCG or Sfinx,the transfected cells displayed a dramatically decreased sensitivity to BbRNT2 or BbTrv,suggesting the requirement of the two transmembrane proteins for BbRNT2 and BbTrv to enter the insect cells.These results reveal a mechanism of the cytotoxic molecules,T2 RNases,produced by the fungal pathogen,entering the insect cells via interaction with specific insect cell transmembrane proteins and causing cytotoxic damage.展开更多
研究了薇甘菊MikaniamicranthaH B K 的乙醇、丙酮提取物对菜青虫Pierisrapae和小菜蛾Plutellaxylostella幼虫的生物活性,结果表明,提取物对菜青虫和小菜蛾幼虫具有较强的毒杀和拒食作用。质量浓度为500mg·L-1的乙醇提取物对1～2...研究了薇甘菊MikaniamicranthaH B K 的乙醇、丙酮提取物对菜青虫Pierisrapae和小菜蛾Plutellaxylostella幼虫的生物活性,结果表明,提取物对菜青虫和小菜蛾幼虫具有较强的毒杀和拒食作用。质量浓度为500mg·L-1的乙醇提取物对1～2龄的菜青虫的毒杀作用达到80%,LC50=293 4mg·L-1对2～3龄小菜蛾幼虫的毒杀作用达到70%以上,LC50=343 5mg·L-1。相同质量浓度的丙酮提取物对2种幼虫的毒杀作用略低于乙醇提取物。质量浓度为125mg·L-1的乙醇、丙酮提取物对2种幼虫均具有拒食作用,前者拒食率分别为97 4%和70 4%,后者提取物的拒食率分别为96 5%和98 3%。薇甘菊(干样 培养基)0 02g mL的甲醇、丙酮提取物对辣椒疫霉Phytophthoracapsili、玉米大斑病菌Exserohilumturcicum菌丝的抑制率均为100%。展开更多
基金the National Natural Science Foundation of China(grant number 81903512)the Fundamental Research Funds for the Central Universities,South-Central Minzu University(Grant Number CPT22033).
文摘Three previously undescribed compounds,cordycicadione(1),cordycicadin F(2),and 7-hydroxybassiatin(3),were isolated from the cultures of Cordyceps cicadae JXCH1,an entomopathogenic fungus.Their structures and relative configurations were elucidated primarily by NMR spectroscopic analysis.The absolute configurations of 1 and 2 were determined by ECD calculations.Single-crystal X-ray diffraction method was adopted to determine the absolute configuration of 3.Compound 2 is a polycyclic polyketide with an unusual enol ether moiety and a spiro ring.The compounds obtained in this study were subjected to screening their inhibition against the proliferation of the human lung cancer cell line A549 and the production of nitric oxide in murine macrophages RAW264.7.
基金supported by the National Natural Science Foundation of China(No.32072485 and 32202378).
文摘Fungal pathogens produce secretory ribonuclease(RNase)T2 proteins during infection,which contribute to fungal virulence via their enzyme functions in degradation of host cell RNA.However,the details of those proteins entering the host cells are unclear.Our previous study demonstrated that the two secretory RNase T2 members,BbRNT2 and BbTrv,produced by the insect fungal pathogen Beauveria bassiana,caused cytotoxic damage to insect cells and contributed to fungal virulence.Here,the Spodoptera frugiperda ovarian epithelial cells(sf9 cells)were used as models to investigate the interactions of the two fungus-produced RNase T2 proteins with the insect cells.Two transmembrane proteins,an ABC transporter(SfABCG)and an Innexin 7-like protein(Sfinx),were identified from the sf9 cells as interacting with BbRNT2 and BbTrv,respectively,through protein immunoprecipitation,yeast-two hybrid tests and protein pull-down assays.Although a slight decrease in the sf9 cell viability was examined by transfection of RNA interference of SfABCG or Sfinx,the transfected cells displayed a dramatically decreased sensitivity to BbRNT2 or BbTrv,suggesting the requirement of the two transmembrane proteins for BbRNT2 and BbTrv to enter the insect cells.These results reveal a mechanism of the cytotoxic molecules,T2 RNases,produced by the fungal pathogen,entering the insect cells via interaction with specific insect cell transmembrane proteins and causing cytotoxic damage.
