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A systematic printability study of direct ink writing towards high-resolution rapid manufacturing 被引量:3
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作者 Qingyang Zheng Bin Xie +1 位作者 Zhoulong Xu Hao Wu 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2023年第3期500-517,共18页
Direct ink writing(DIW)holds enormous potential in fabricating multiscale and multi-functional architectures by virtue of its wide range of printable materials,simple operation,and ease of rapid prototyping.Although i... Direct ink writing(DIW)holds enormous potential in fabricating multiscale and multi-functional architectures by virtue of its wide range of printable materials,simple operation,and ease of rapid prototyping.Although it is well known that ink rheology and processing parameters have a direct impact on the resolution and shape of the printed objects,the underlying mechanisms of these key factors on the printability and quality of DIW technique remain poorly understood.To tackle this issue,we systematically analyzed the printability and quality through extrusion mechanism modeling and experimental validating.Hybrid non-Newtonian fluid inks were first prepared,and their rheological properties were measured.Then,finite element analysis of the whole DIW process was conducted to reveal the flow dynamics of these inks.The obtained optimal process parameters(ink rheology,applied pressure,printing speed,etc)were also validated by experiments where high-resolution(<100μm)patterns were fabricated rapidly(>70 mm s^(-1)).Finally,as a process research demonstration,we printed a series of microstructures and circuit systems with hybrid inks and silver inks,showing the suitability of the printable process parameters.This study provides a strong quantitative illustration of the use of DIW for the high-speed preparation of high-resolution,high-precision samples. 展开更多
关键词 direct ink writing extrusion mechanism modelling computational fluid dynamic(CFD) printability process parameters high-resolution printing
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3D printing of architectured graphene-based aerogels by cross-linking GO inks with adjustable viscoelasticity for energy storage devices 被引量:2
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作者 San-Can Han Jia-Le Quan +4 位作者 Fu-Guo Zhou Yu-Hua Xue Na Li Feng-Yu Li Ding Wang 《Rare Metals》 SCIE EI CAS CSCD 2023年第3期971-981,共11页
Three-dimensional(3D)functional graphenebased architecture with superior electrical conductivity and good mechanical strength has promising applications in energy storage and electrics.Viscoelasticity-adjustable inks ... Three-dimensional(3D)functional graphenebased architecture with superior electrical conductivity and good mechanical strength has promising applications in energy storage and electrics.Viscoelasticity-adjustable inks make it possible to achieve desired 3D architectures with interconnected and continuous interior networks by microextrusion printing.In this work,ultra-low-concentration graphene oxide(GO)inks of~15 mg·ml-1 have been obtained and demonstrated in direct 3D printing with a facile cross-linking(direct ink writing).The rheological behavior of the GO strategy by cations,which is the lowest concentration to achieve direct ink writing inks,could be adjusted from 1×10^(4) to 1×10^(5) Pa·s^(-1) with different concentrations of cations due to strong cross-linking networks between GO sheets and cations.Meanwhile,the specific strength and electrical conductivity of 3D-printed graphene architecture are notably enhanced,reaching up to 51.7×10^(3) N·m·kg^(-1)and 119 S·m^(-1),which are superior to conventional graphene aerogels.Furthermore,3D printing graphene-based architecture assembled in micro-superc apacitor exhibits excellent electrochemical performance,which can be ascribed to the effective ion transportation through the interconnected networks.The strategy demonstrated is useful in the design of complex-shaped,graphene-based architectures for scalable manufacturing of practical energy storage applications. 展开更多
关键词 Three-dimensional(3D)printing Graphene oxide(GO)inks Cross-linking strategy Graphene-based architecture
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An in-situ hybrid laser-induced integrated sensor system with antioxidative copper 被引量:3
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作者 Kaichen Xu Zimo Cai +5 位作者 Huayu Luo Xingyu Lin Geng Yang Haibo Xie Seung Hwan Ko Huayong Yang 《International Journal of Extreme Manufacturing》 CSCD 2024年第6期535-546,共12页
Integration of sensors with engineering thermoplastics allows to track their health and surrounding stimuli.As one of vital backbones to construct sensor systems,copper(Cu)is highly conductive and cost-effective,yet t... Integration of sensors with engineering thermoplastics allows to track their health and surrounding stimuli.As one of vital backbones to construct sensor systems,copper(Cu)is highly conductive and cost-effective,yet tends to easily oxidize during and after processing.Herein,an in-situ integrated sensor system on engineering thermoplastics via hybrid laser direct writing is proposed,which primarily consists of laser-passivated functional Cu interconnects and laser-induced carbon-based sensors.Through a one-step photothermal treatment,the resulting functional Cu interconnects after reductive sintering and passivation are capable of resisting long-term oxidation failure at high temperatures(up to 170℃)without additional encapsulations.Interfacing with signal processing units,such an all-in-one system is applied for long-term and real-time temperature monitoring.This integrated sensor system with facile laser manufacturing strategies holds potentials for health monitoring and fault diagnosis of advanced equipment such as aircrafts,automobiles,high-speed trains,and medical devices. 展开更多
关键词 hybrid laser direct writing in-situ integrated sensor systems engineering thermoplastics functional copper inks laser-induced passivation
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Research on HCI Prototype Design of Ink Automatic Control System for Offset Printer
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作者 ZHANG Jianyu~1 GAO Lixin~1 CUI Lingli~1 WANG Yingwang~2 LI Xianghui~2 1.Key Laboratory of Advanced Manufacturing Technology,Beijing University of Technology,Beijing 100022,China, 2.Tangshan Iron and Steel Corp.LTD,Tangshan 063000,China 《武汉理工大学学报》 CAS CSCD 北大核心 2006年第S2期772-776,共5页
In order to solve the low efficiency and poor precision problems of traditional ink control methods on domestic offset printers,developing modern ink automatic control system has become more and more urgent.As an impo... In order to solve the low efficiency and poor precision problems of traditional ink control methods on domestic offset printers,developing modern ink automatic control system has become more and more urgent.As an important subsystem,the hu- man computer interface (HCI) is a key function for the wholly automatic control.Once this goal is obtained,all the printing pro- cedures especially the automatic control of ink volume should be finished with human computer interface in different areas.consid- ering the HCI design theory and structure characteristics of domestic printers comprehensively,the HCI prototype for automatic ink control system has been developed based on Visual Basic platform.As the individual ink key is instead of the integrated key,the di- vision result of ink fountain can be displayed on the interface.Through the interface,the dynamic adjusting functions such as mod- ifying ink volume,locking or unlocking each ink key and real-time displaying the adjusting results etc.can be completed.The sim- ulation test has shown that the opening and practical feature of the prototype is satisfactory. 展开更多
关键词 PRINTING machine INK automatic control human COMPUTER interface
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Forming mechanism of ink layer on the printing plate in inking process and influencing factors of its thickness
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作者 初红艳 Xu Kangjian +1 位作者 Zhang Xiaolin Cai Ligang 《High Technology Letters》 EI CAS 2016年第3期297-304,共8页
Ink layer thickness on the printing plate greatly influences uniformity of ink transferred to the substrates,which is an important indicator of printing quality,so the study of ink layer and its thickness is important... Ink layer thickness on the printing plate greatly influences uniformity of ink transferred to the substrates,which is an important indicator of printing quality,so the study of ink layer and its thickness is important for improving the quality of printing products. Ansys CFX is used here to build a model of ink fluid adhering to lower vibrator roller,form inking roller,and printing plate for analyzing ink transferring in inking process. Ink layer thickness on each position of the model is acquired to analyze the forming mechanism of ink layer on printing plate,as well as the influence of oscillation speed of lower vibrator roller and dot area percentage of plate on ink layer thickness of printing plate. It can be concluded that,in the case of fixed ink supplying amount,ink layer thickness increases along with the increasing of oscillation speed,and decreases when the dot area percentage is getting larger and the minimum is got when the dot area percentage is 100%. At last,experiment of plate inking on print ability tester verifies the correctness of the simulation analysis. 展开更多
关键词 PRINTING OSCILLATION dot area percentage ink layer thickness
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Comparisons between Flotation Deinking Abilities of Newsprint Paper by Using Enzyme and Chemicals
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作者 Nucharin Luangsa-Ard Oranutch Khampan Somporn Chaiareekij 《Journal of Chemistry and Chemical Engineering》 2012年第9期832-837,共6页
The aim of this research is to study the floatation deinking abilities of black mold enzyme and chemicals in newsprint paper application. In this experiment, the properties of deinked pulp from three different additiv... The aim of this research is to study the floatation deinking abilities of black mold enzyme and chemicals in newsprint paper application. In this experiment, the properties of deinked pulp from three different additives--chemicals, black mold enzyme, and chemicals together with black mold enzyme, are studied. The first part of the experiment was to find the optimal amount of chemicals and the optimal pH through the use of sodium hydroxide and surfactant. As a result, 0.2% of sodium hydroxide and 0.4% of surfactant on oven dried weight at pH 9 was found to be the optimal condition for the deinked pulp to yield the lowest ERIC (effective residual concentration) and the highest brightness. The second part of the experiment was to find the optimal amount of black mold enzyme used in the deinking process. As a result, the optimal condition for deinked pulp to retain the lowest ERIC and the highest brightness was 100 ppm of black mold enzyme and 10 minutes of enzyme reaction time. The third part of experiment was to study the de-inking ability of black mold enzyme and chemicals. Consequently, with 30 min of chemicals reaction time and 60 min of black mold reaction time, the deinked pulp retained low ERIC but higher brightness. 展开更多
关键词 NEWSPRINT DEinking ENZYME offset ink.
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The developing Guangdong Electric Power System
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作者 Zhang Yuqi Power Bureau of Guangdong Province 《Electricity》 1992年第1期51-54,共4页
Guangdong,a coastal province in south China,hadvery poor foundation of industry in the past.Afterthe establishment of People’s Republic of China in 1949,under theleadership and unified planning of the central governm... Guangdong,a coastal province in south China,hadvery poor foundation of industry in the past.Afterthe establishment of People’s Republic of China in 1949,under theleadership and unified planning of the central government,economyconstruction of both industry and agriculture have been boosted andhence the provicial electric power industry have developed accord-ingly.Especially since 1979,the implementation of reformation 展开更多
关键词 GUANGDONG ACCORD agriculture unified REFORMATION OWNED inked VENTURE FACTORY county
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ZTE Inks Strategic Agreements with Two Leading European Telecom System Integrators
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《ZTE Communications》 2008年第1期61-61,共1页
ZTE Corporation has signed strategic telecommunications software agreement with two leading providers in Europe and Latin America to optimize its offerings for target customers in
关键词 ZTE Inks Strategic Agreements with Two Leading European Telecom system Integrators
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结肠癌合并2型糖尿病患者血清lncRNA ANRIL、miR-181b-5p的表达及其与淋巴结转移的相关性
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作者 潘勇娜 康金旺 常月锋 《实用临床医药杂志》 2025年第2期32-37,共6页
目的探讨结肠癌合并2型糖尿病(T2DM)患者血清长链非编码RNA INK4基因座中反义非编码RNA(lncRNA ANRIL)、微小RNA-181b-5p(miR-181b-5p)的表达及其与淋巴结转移的相关性。方法选取2019年5月—2022年8月本院收治的117例结肠癌合并T2DM患... 目的探讨结肠癌合并2型糖尿病(T2DM)患者血清长链非编码RNA INK4基因座中反义非编码RNA(lncRNA ANRIL)、微小RNA-181b-5p(miR-181b-5p)的表达及其与淋巴结转移的相关性。方法选取2019年5月—2022年8月本院收治的117例结肠癌合并T2DM患者为结肠癌合并T2DM组,并根据患者淋巴结转移情况将其分为淋巴结转移组56例和淋巴结未转移组61例。另选取同期体检的健康志愿者117例为对照组。采用反转录实时定量PCR检测血清lncRNA ANRIL、miR-181b-5p的相对表达水平。采用Logistic回归分析探讨影响结肠癌合并T2DM患者淋巴结转移的因素。绘制受试者工作特征(ROC)曲线分析血清lncRNA ANRIL、miR-181b-5p水平对结肠癌合并T2DM患者淋巴结转移的诊断价值。采用Pearson相关分析探讨结肠癌合并T2DM患者血清lncRNA ANRIL与miR-181b-5p表达的相关性。结果与对照组比较,结肠癌合并T2DM组患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,差异均有统计学意义(P<0.01)。与淋巴结未转移组比较,淋巴结转移组患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,差异均有统计学意义(P<0.01)。淋巴结转移组与淋巴结未转移组患者TNM分期、分化程度以及糖化血红蛋白(HbA1C)比较,差异有统计学意义(P<0.05)。糖尿病史、TNM分期、分化程度、HbA1C以及lncRNA ANRIL均是淋巴结转移的影响因素(P<0.05),miR-181b-5p是影响淋巴结转移的保护因素(P<0.05)。血清lncRNA ANRIL与miR-181b-5p水平呈负相关(r=-0.440,P<0.001)。ROC曲线结果显示,血清lncRNA ANRIL、miR-181b-5p以及二者联合诊断淋巴结转移的曲线下面积(AUC)分别为0.800、0.837、0.930,联合诊断价值显著高于lncRNA ANRIL(Z=2.956,P=0.003)、miR-181b-5p(Z=2.117,P=0.034)单独诊断价值。结论结肠癌合并T2DM患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,且二者与淋巴结转移具有一定相关性。 展开更多
关键词 结肠癌 2型糖尿病 长链非编码RNA INK4基因座中反义非编码RNA 微小RNA-181b-5p
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益气解毒方下调ANRIL抑制鼻咽癌细胞增殖、迁移与侵袭
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作者 许红淼 周芳亮 +5 位作者 何兰 左涵蓉 邹芳 唐乐 江志超 何迎春 《中国中西医结合杂志》 北大核心 2025年第11期1386-1392,共7页
目的研究益气解毒方(YQJDF)是否通过下调INK4基因座中反义非编码RNA(ANRIL)干预鼻咽癌细胞的生物学效应。方法运用慢病毒转染技术构建ANRIL过表达和敲低的鼻咽癌稳定转染细胞株,再采用0.5 g/L YQJDF干预,将细胞分为过表达ANRIL(oe-ANRIL... 目的研究益气解毒方(YQJDF)是否通过下调INK4基因座中反义非编码RNA(ANRIL)干预鼻咽癌细胞的生物学效应。方法运用慢病毒转染技术构建ANRIL过表达和敲低的鼻咽癌稳定转染细胞株,再采用0.5 g/L YQJDF干预,将细胞分为过表达ANRIL(oe-ANRIL)+YQJDF组、过表达空载体(oe-NC)+YQJDF组、干扰ANRIL(sh-ANRIL)+YQJDF组、干扰空载体(sh-NC)+YQJDF组,每组设3个重复。实时细胞分析技术(RTCA)检测细胞增殖能力,划痕实验检测细胞迁移能力,Matrigel侵袭小室法检测细胞侵袭能力RT-PCR检测ANRIL的表达水平。结果RTCA检测显示,与空载体(oe-NC)组比较,oe-ANRIL组细胞的增殖速度增高(P<0.01),相对增殖率提高35%。划痕与侵袭实验进一步证实,oe-ANRIL细胞迁移和侵袭能力增强(P<0.01)。与oe-NC+YQJDF组比较,过表达ANRIL后,益气解毒方抑制细胞增殖、迁移和侵袭的效应均降低(P<0.05);沉默ANRIL后,YQJDF抑制细胞增殖、迁移效应均增强(P<0.05)。结论ANRIL促进鼻咽癌细胞的增殖、迁移与侵袭,YQJDF通过靶向抑制ANRIL的表达发挥抗鼻咽癌的作用。 展开更多
关键词 益气解毒方 鼻咽癌 INK4基因座中反义非编码RNA 侵袭 迁移 中药复方 中西医结合
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Synthesis of p-type PbS quantum dot ink via inorganic ligand exchange in solution for high-efficiency and stable solar cells 被引量:1
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作者 Napasuda Wichaiyo Yuyao Wei +9 位作者 Chao Ding Guozheng Shi Witoon Yindeesuk Liang Wang Huān Bì Jiaqi Liu Shuzi Hayase Yusheng Li Yongge Yang Qing Shen 《Journal of Semiconductors》 2025年第4期63-70,共8页
Traditional p-type colloidal quantum dot(CQD)hole transport layers(HTLs)used in CQD solar cells(CQDSCs)are commonly based on organic ligands exchange and the layer-by-layer(LbL)technique.Nonetheless,the ligand detachm... Traditional p-type colloidal quantum dot(CQD)hole transport layers(HTLs)used in CQD solar cells(CQDSCs)are commonly based on organic ligands exchange and the layer-by-layer(LbL)technique.Nonetheless,the ligand detachment and complex fabrication process introduce surface defects,compromising device stability and efficiency.In this work,we propose a solution-phase ligand exchange(SPLE)method utilizing inorganic ligands to develop stable p-type lead sulfide(PbS)CQD inks for the first time.Various amounts of tin(Ⅱ)iodide(SnI_(2))were mixed with lead halide(PbX_(2);X=I,Br)in the ligand solution.By precisely controlling the SnI_(2)concentration,we regulate the transition of PbS QDs from n-type to p-type.PbS CQDSCs were fabricated using two different HTL approaches:one with 1,2-ethanedithiol(EDT)-passivated QDs via the LbL method(control)and another with inorganic ligand-passivated QD ink(target).The target devices achieved a higher power conversion efficiency(PCE)of 10.93%,compared to 9.83%for the control devices.This improvement is attributed to reduced interfacial defects and enhanced carrier mobility.The proposed technique offers an efficient pathway for producing stable p-type PbS CQD inks using inorganic ligands,paving the way for high-performance and flexible CQD-based optoelectronic devices. 展开更多
关键词 quantum dot solar cells hole transport layer PBS p-type ink inorganic ligands
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4D-printed snake-like biomimetic soft robots 被引量:1
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作者 Xingcheng Ou Jiaqi Huang +6 位作者 Dantong Huang Xiaohong Li Guoliang Chen Yabin Yang Ran Bi Yu Sheng Shuang-Zhuang Guo 《Bio-Design and Manufacturing》 2025年第1期55-67,I0018-I0038,共34页
Wireless millirobots engineered to infiltrate intricate vascular networks within living organisms,particularly within constricted and confined spaces,hold immense promise for the future of medical treatments.However,w... Wireless millirobots engineered to infiltrate intricate vascular networks within living organisms,particularly within constricted and confined spaces,hold immense promise for the future of medical treatments.However,with their multifaceted and intricate designs,some robots often grapple with motion and functionality issues when confronted with tight spaces characterized by small cross-sectional dimensions.In this study,drawing inspiration from the high aspect ratio and undulating swimming patterns of snakes,a millimeter-scale,snake-like robot was designed and fabricated via a combination of extrusion-based four-dimensional(4D)printing and magnetic-responsive intelligent functional inks.A sophisticated motion control strategy was also developed,which enables the robots to perform various dynamic movements,such as undulating swimming,precise turns,graceful circular motions,and coordinated cluster movements,under diverse magnetic field variations.As a potential application,the snake robot can navigate and release drugs in a model coronary intervention vessel with tortuous channels and fluid filling.The novel design and promising applications of this snake robot are invaluable tools in future medical surgeries and interventions. 展开更多
关键词 4D printing Magnetic-responsive ink Untethered medical soft robot Snake-like robot Drug delivery
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胆囊癌组织p16INK4a基因甲基化状态和蛋白表达及其与预后的相关性 被引量:1
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作者 石明亮 王晓磊 +2 位作者 郭伟 朱硕 申洋 《社区医学杂志》 2025年第9期292-298,共7页
目的探讨p16INK4a基因甲基化状态及蛋白表达与胆囊癌临床特征及其与预后的相关性。方法收集2018-01-01-2019-12-30河南大学第一附属医院收治的115例胆囊癌患者的临床资料,采用直接测序法检测癌组织基因组DNA p16INK4a基因甲基化水平,免... 目的探讨p16INK4a基因甲基化状态及蛋白表达与胆囊癌临床特征及其与预后的相关性。方法收集2018-01-01-2019-12-30河南大学第一附属医院收治的115例胆囊癌患者的临床资料,采用直接测序法检测癌组织基因组DNA p16INK4a基因甲基化水平,免疫组化法检测组织p16INK4a蛋白表达;分析不同临床病理特征癌组织p16INK4a基因甲基化状态及蛋白表达差异;Kaplan-Meier法分析不同p16INK4a基因甲基化状态及蛋白表达者生存差异;Cox比例风险模型分析胆囊癌患者预后影响因素。结果胆囊癌组织p16INK4a基因甲基化阳性率为56.52%(65/115),高于癌旁组织的18.26%(21/115),χ^(2)=35.956,P<0.001。胆囊癌组织p16INK4a蛋白表达阳性率为26.09%(30/115),低于癌旁组织61.74%(71/115),χ^(2)=24.305,P<0.001。低中分化、Ⅲ期患者的癌组织p16INK4a基因甲基化阳性率高于高分化、Ⅰ~Ⅱ期,p16INK4a蛋白阳性表达率低于高分化、Ⅰ~Ⅱ期,均P<0.05。随访期间73例患者死亡,p16INK4a蛋白阳性表达(63.33%)、p16INK4a基因甲基化阴性(54.00%)胆囊癌患者3年总生存率高于p16INK4a蛋白阴性表达(27.06%)、p16INK4a基因甲基化阳性23.07%。TNM分期(HR=3.572,95%CI:1.995~6.393)、淋巴结转移(HR=2.396,95%CI:1.317~4.356)、组织分化程度(HR=2.628,95%CI:1.606~4.298)、p16INK4a基因甲基化(HR=1.915,95%CI:1.362~2.693)、p16INK4a蛋白(HR=0.773,95%CI:0.659~0.906)是胆囊癌死亡的影响因素,均P<0.05。结论p16INK4a基因启动子甲基化状态及蛋白表达与胆囊癌TNM分期、组织分化程度及预后有关,可能参与胆囊癌发生发展。 展开更多
关键词 胆囊癌 P16INK4A DNA甲基化 免疫组织化学 预后因素
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T_(2)mapping对止痛健骨方防治兔膝骨关节炎关节软骨衰老疗效的监测
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作者 高辉 黎芳丽 +1 位作者 颜路悠 张堃 《中国中西医结合影像学杂志》 2025年第4期456-460,共5页
目的:评估止痛健骨方防治膝骨关节炎关节软骨衰老的疗效,探讨T_(2)mapping对疗效监测的价值。方法:采用石膏绷带伸直位制动膝关节的方法制作新西兰大白兔膝骨关节炎模型,将32只新西兰大白兔随机分为正常对照组、模型对照组、止痛健骨方... 目的:评估止痛健骨方防治膝骨关节炎关节软骨衰老的疗效,探讨T_(2)mapping对疗效监测的价值。方法:采用石膏绷带伸直位制动膝关节的方法制作新西兰大白兔膝骨关节炎模型,将32只新西兰大白兔随机分为正常对照组、模型对照组、止痛健骨方组及硫酸氨基葡萄糖组各8只。制动2周后,正常对照组和模型对照组给予相同容积的蒸馏水灌胃,止痛健骨方组给予止痛健骨方,硫酸氨基葡萄糖组给予硫酸及葡萄糖胶囊。治疗4周后均行膝关节T_(2)mapping成像,并对关节软骨细胞的p16^(INK4a)表达量进行免疫组化分析。比较各组间膝关节软骨T_(2)值及p16^(INK4a)表达量的差异,并分析不同干预方法、关节软骨T_(2)值与关节软骨细胞p16^(INK4a)表达量的相关性。结果:止痛健骨方组、硫酸氨基葡萄糖组关节软骨T_(2)值均高于正常对照组,低于模型对照组(均P<0.05);正常对照组、止痛健骨方组、硫酸氨基葡萄糖组关节软骨细胞p16^(INK4a)表达量均明显低于模型对照组(均P<0.05),但3组间差异无统计学意义(P>0.05)。软骨细胞p16^(INK4a)表达量与关节软骨T_(2)值呈非线性关系,p16^(INK4a)表达量随T_(2)值升高,先缓慢升高,再迅速升高(P<0.05)。结论:止痛健骨方可防治膝骨关节炎关节软骨衰老,且T_(2)mapping可作为药物防治软骨衰老的无创评估工具。 展开更多
关键词 骨性关节炎 关节软骨 止痛健骨方 磁共振成像 p16^(INK4a)
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INK4基因座中反义非编码RNA对乳腺癌细胞增殖能力及顺铂敏感性的影响
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作者 韩一菲 张芳芳 +4 位作者 王靖楠 邹炎 应莉 谢依天 李娜 《新乡医学院学报》 2025年第2期93-98,106,共7页
目的探讨INK4基因座中反义非编码RNA(ANRIL)对乳腺癌细胞增殖能力及顺铂敏感性的影响及其作用机制。方法采用实时荧光定量聚合酶链反应(RT-qPCR)技术检测乳腺癌组织标本及配对的癌旁组织、人乳腺癌细胞株MCF-7、MDA-MB-231、SKBR3以及... 目的探讨INK4基因座中反义非编码RNA(ANRIL)对乳腺癌细胞增殖能力及顺铂敏感性的影响及其作用机制。方法采用实时荧光定量聚合酶链反应(RT-qPCR)技术检测乳腺癌组织标本及配对的癌旁组织、人乳腺癌细胞株MCF-7、MDA-MB-231、SKBR3以及人正常乳腺上皮细胞株MCF-10A中ANRIL的表达;将对数生长期的MDA-MB-231细胞随机分为si-NC组(转染对照的空载siRNA)、si-ANRIL组(转染ANRIL干扰片段)、si-ANRIL-anti-NC组(共转染ANRIL的干扰片段和miR-7-5p的空载体)、si-ANRIL-anti-miR-7-5p组(转染ANRIL的干扰片段和miR-7-5p的干扰片段)、miR-NC组(转染对照miRNA的空载体)、miR-7-5p mimic组(转染miR-7-5p的过表达载体)、miR-7-5p+ANRIL-WT转染组(共转染miR-7-5p mimics和ANRIL-WT)、miR-NC+ANRIL-WT转染组(共转染mimics Control和ANRIL-WT)、miR-7-5p+ANRIL-MUT转染组(共转染miR-7-5p mimics和ANRIL-MUT)和miR-NC+ANRIL-WUT转染组(共转染mimics Control和ANRIL-MUT)。采用细胞计数试剂盒-8法检测乳腺癌细胞的体外增殖能力,克隆形成实验检测细胞对顺铂的敏感性,双荧光素酶报告实验和RNA免疫沉淀(RIP)实验检测ANRIL和miR-7-5p的靶向结合关系。结果乳腺癌组织中ANRIL mRNA相对表达量显著高于癌旁组织(P<0.05);MDA-MB-231、MCF-7和SKBR3细胞中ANRIL mRNA的相对表达量显著高于正常乳腺上皮细胞(P<0.05);MDA-MB-231细胞中ANRIL mRNA的表达量显著高于MCF-7和SKBR3细胞(P<0.05)。培养24、48、72 h时,si-ANRIL组细胞存活率显著低于si-NC组(P<0.05)。与si-NC组相比,si-ANRIL组细胞克隆形成数目显著降低(P<0.05)。与miR-NC+ANRIL-WT转染组相比,miR-7-5p+ANRIL-WT转染组细胞荧光素酶活性显著降低(P<0.05);miR-NC+ANRIL-MUT转染组与miR-7-5p+ANRIL-MUT转染组细胞荧光素酶活性比较差异无统计学意义(P>0.05)。miR-7-5p mimic组细胞中ANRIL富集量显著高于miR-NC组(P<0.05)。在培养24、48、72 h时,si-ANRIL-anti-miR-7-5p组MDA-MB-231细胞存活率显著高于si-ANRIL-anti-NC组(P<0.05)。si-ANRIL-anti-miR-7-5p组MDA-MB-231细胞克隆形成数目显著高于si-ANRIL-anti-NC组(P<0.05)。与si-ANRIL-anti-NC组相比,si-ANRIL-anti-miR-7-5p组的MDA-MB-231细胞对顺铂的敏感性降低了4.04倍。结论ANRIL在乳腺癌组织和乳腺癌细胞中呈高表达,并通过下调miR-7-5p的表达来促进乳腺癌细胞的增殖,降低乳腺癌细胞对顺铂的敏感性。 展开更多
关键词 INK4基因座中反义非编码RNA 微RNA-7-5p 乳腺癌 生物学行为 顺铂敏感性
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Constructing Ti_(3)C_(2)T_(x)-MXene-based gradient woodpile structure by direct ink writing 3D printing for efficient microwave absorption 被引量:1
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作者 Changtian Zhu Pei Liu +8 位作者 Jin Chen Zixuan Ding Guohui Tang Qingqing Gao Yinxu Ni Kai Xu Zhilei Hao Gaojie Xu Fenghua Liu 《International Journal of Minerals,Metallurgy and Materials》 2025年第3期657-667,共11页
As a novel 2D material,Ti_(3)C_(2)T_(x)-MXene has become a major area of interest in the field of microwave absorption(MA).However,the MA effect of common Ti_(3)C_(2)T_(x)-MXene is not prominent and often requires com... As a novel 2D material,Ti_(3)C_(2)T_(x)-MXene has become a major area of interest in the field of microwave absorption(MA).However,the MA effect of common Ti_(3)C_(2)T_(x)-MXene is not prominent and often requires complex processes or combinations of other ma-terials to achieve enhanced performance.In this context,a kind of gradient woodpile structure using common Ti_(3)C_(2)T_(x)-MXene as MA ma-terial was designed and manufactured through direct ink writing(DIW)3D printing.The minimum reflection loss(RL_(min))of the Ti_(3)C_(2)T_(x)-MXene-based gradient woodpile structures with a thickness of less than 3 mm can reach-70 dB,showing considerable improve-ment compared with that of a completely filled structure.In addition,the effective absorption bandwidth(EAB)reaches 7.73 GHz.This study demonstrates that a Ti_(3)C_(2)T_(x)-MXene material with excellent MA performance and tunable frequency band can be successfully fab-ricated with a macroscopic structural design and through DIW 3D printing without complex material hybridization and modification,of-fering broad application prospects by reducing electromagnetic wave radiation and interference. 展开更多
关键词 Ti_(3)C_(2)T_(x)-MXene electromagnetic wave absorption macro-design impedance matching 3D printing ink
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Research on Printing Preferences for Skin Color
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作者 WANG Dan GONG Xu-ping +1 位作者 SHANG Xiao-yu HUANG Min 《印刷与数字媒体技术研究》 北大核心 2025年第1期46-52,共7页
In order to study the preferred skin color for printing images,two CMYK images from ISO 400 and one from iStock,including five skin color images of East Asian females was selected in this study.The images were adjuste... In order to study the preferred skin color for printing images,two CMYK images from ISO 400 and one from iStock,including five skin color images of East Asian females was selected in this study.The images were adjusted with the CMYK printing ink volume variation of the single,double and triple channels in the given 280%total ink limit conditions.A larger number of color vision normal observers were organized to carry out the color preference evaluation experiment,and the selected preferred skin colors were analyzed.The distribution range of the chromaticity values for skin color images were obtained and the results indicated that there are three regions for printing skin color preferences,and the observers have a memory preference for brighter,fairer skin colors in young female and a reddish skin colors in girl,which can provide the guidance for color adjustment of printed skin color images. 展开更多
关键词 Preferred skin color Printing images Total ink limit CMYK ink adjustment
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An Initial Exploration of Digital Ink Texture Construction Methods Based on Blender Material Nodes
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作者 XIN Bei XU Zhendong 《Cultural and Religious Studies》 2025年第1期26-32,共7页
This study examines the development of painting techniques of Chinese ink wash landscape paintings,pays attention to its unique brush and ink language and features of the representation of elements,and deeply analyzes... This study examines the development of painting techniques of Chinese ink wash landscape paintings,pays attention to its unique brush and ink language and features of the representation of elements,and deeply analyzes the artistic characteristics of digital ink wash texture materials.The research focuses on key aspects such as the ink brushstrokes with the combination of emptiness and reality,the profound and serene ink wash space,and the extremely ingenious position layout.It proposes a construction path of digital ink wash texture materials based on the Blender material node system.This method makes use of the flexibility of the Blender material node system to successfully simulate highly realistic digital ink wash textures.It can not only construct static ink wash textures but also realize the dynamic transformation of static ink wash works through animation nodes and procedural control,thereby enhancing the artistic expression of digital ink wash works.The proposal and implementation of this method expand the application scope of the Blender material node system,help deeply explore the potential of digital ink wash art,and open up a brand new research path for constructing digital ink wash textures. 展开更多
关键词 Blender material node digital ink ink textures
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Developments and Challenges in Direct Ink Writing for High-Performance Polymers
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作者 Zhangzhang Tang Yaoming Zhang +4 位作者 Liming Tao Zenghui Yang Peng Liu Qihua Wang Tingmei Wang 《Additive Manufacturing Frontiers》 2025年第2期111-133,共23页
Compared to subtractive manufacturing and casting,3D printing(additive manufacturing)offers advantages,such as the rapid production of complex structures,reduced material waste,and environmental friendliness.Direct in... Compared to subtractive manufacturing and casting,3D printing(additive manufacturing)offers advantages,such as the rapid production of complex structures,reduced material waste,and environmental friendliness.Direct ink writing(DIW)is one of the most popular 3D printing techniques owing to its ability to print multiple materials simultaneously and its high compatibility with printing inks.However,DIW presents significant challenges,particularly in the printing of high-performance polymers.The main challenges are as follows:1.The rigid structures and reaction kinetics of high-performance polymers make developing new inks difficult.2.The limited types of available high-performance polymers underscore the need for new DIW-suitable materials.3.Layer-by-layer stacking weakens interlayer bonding,affecting the mechanical properties of the printed product.4.The accuracy and speed of DIW printing are insufficient for large-scale manufacturing.After introducing the topic,the requirements for DIW printing inks are first reviewed,emphasizing the importance of thixotropic agents.Then,research progress regarding DIW printing of high-performance polymers is comprehensively reviewed according to the requirements of different polymer inks.Additionally,the applications of these materials across various fields are summarized.Finally,the challenges in DIW printing of high-performance polymers,along with corresponding solutions and future development prospects,are discussed in detail. 展开更多
关键词 3D printing Direct ink writing Printing ink Polymer material High-performance polymer
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WHICH IS BETTER
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《空中英语教室(初级版.大家说英语)》 2025年第9期20-20,56,共2页
Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can d... Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can dry out,but pencil lead doesn’t.Sam:But words look neater when you write in pen.Susie:They do.So,which pen do you want?Sam:One with blue ink,thanks. 展开更多
关键词 DRAWING PENCILS INK lead PENS WRITING
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