The prebiotic effects of hyaluronan(HA)are widely recognized,contributing to improved gut health and immune modulation.Despite its extensive use as dietary supplement,the specific interactions between HA oligosacchari...The prebiotic effects of hyaluronan(HA)are widely recognized,contributing to improved gut health and immune modulation.Despite its extensive use as dietary supplement,the specific interactions between HA oligosaccharides(o-HAs)and the gut microbiome remain largely unexplored.To investigate its role and metabolic fate in gut homeostasis,200 mg/day of o-HAs(average molecule weight 1 kDa)were added to an automated computer-controlled SIMulator of the Gastrointestinal tract(SIMGI).The results revealed a significant reshaping of the intestinal flora composition by o-HAs,notably reducing the Firmicutes/Bacteroides ratio.Fermentation of o-HAs by gut microbiota significantly increased the abundance of Bifidobacterium,Prevotellaceae_Prevotella,Dialister,Eubacterium,and Sutterella,but decreased that of Catenibacterium,Oscillospira,Klebsiella,and Citrobacter(P<0.05).This corresponded with significant enhancements in the content of short-chain fatty acids(SCFAs)such as acetic acid,propionic acid and n-butyric acid,highlighting the significant impact of o-HAs at the genus level.Furthermore,analysis of microbial function predicted the downregulation of pathological events in nine human diseases,particularly infectious ones(parasitic and bacterial).Potential inhibitions were observed in metabolic pathways associated with pentose and glucuronate interconversions as well as cationic antimicrobial peptide resistance.These findings underscore the in vitro prebiotic effects of o-HAs and their potential relevance in managing diverticular diseases or preventing metabolic disorders through the regulation of gut microbiota.展开更多
To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subseq...To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.展开更多
Beer fermentation process is a complex biochemical reaction process.It is the most important to control temperature of the wort in fermentation tank in accordance with the beer fermentation temperature curve so as to ...Beer fermentation process is a complex biochemical reaction process.It is the most important to control temperature of the wort in fermentation tank in accordance with the beer fermentation temperature curve so as to ensure the completion of fermentation.The controlled object is characterized by large inertia,long time delay and mutual coupling of three temperature areas.Based on this,a temperature control method for beer fermentation system is designed.Using digital incremental proportion integration differentiation （PID） control algorithm,the controlled quantity is transmitted to the controlled object after diagonal matrix decoupling.This simulation system can be completed in laboratory using VB and Kingview software,so it has the features of good security and low cost.It is very suitable for experimental teaching.展开更多
The feasibility of ethanol separation from ethanol-water solution saturated with glucose by gas strippingis showil theoretically and experimentally. The effects of glucose on the saturated vapor pressure of ethanol, a...The feasibility of ethanol separation from ethanol-water solution saturated with glucose by gas strippingis showil theoretically and experimentally. The effects of glucose on the saturated vapor pressure of ethanol, and thatof operating temperature and gas stripping flow rates, K/G, on Stripping result are discussed.展开更多
OBJECTIVE Epimedium is rich in a variety of beneficial active ingredients,and has been widely used in the ethnopharmacological practices,however,its biotransformation in gastrointestinal digestions remain unclear.This...OBJECTIVE Epimedium is rich in a variety of beneficial active ingredients,and has been widely used in the ethnopharmacological practices,however,its biotransformation in gastrointestinal digestions remain unclear.This study aimed to investigate the dynamic changes of components and biological activity of Epimedium in the in vitro simulated digestion and subsequent human faecal fermentation.METHODS The models of in vitro simulated saliva,gastric and intestinal digestion,as well as colonic fermentation were constructed to simulate the digestion process of Epimedium.The dynamic changes of components of Epimedium during the simulated digestions in vitro and subsequent human faecal fermentation were investigated by UPLC-MS,HPLC-DAD combined with principal component analysis(PCA)and multi-ingredient quantitative analysis.RESULTS A variety of metabolites with high contents were produced after 0.5 h of intestinal digestion and colonic fermentation 0.5 h.Application of PCA to HPLC data showed the obvious separation of colonic fermentation 0.5 h stage samples from other colonic fermentation stages samples(24,48 and 72 h).Additionally,non-digestion and saliva digestion stage samples clustered together,and there was obvious separation between intestinal digestion samples and gastric digestion samples.The contents of epimedium C,icariin and baohuside I all increased significantly after intestinal digestion[58.70±7.08,47.15±5.68 and(12.78±0.55)mg·g^(-1)]compared with gastric digestion[29.00±5.65,17.40±4.55 and(2.77±0.19)mg·g^(-1)].There were significant differences between sample after 0.5 h of colonic fermentation[64.22±9.32,51.26±6.33 and(16.68±3.19)mg·g^(-1)]and other time points(24,48 and 72 h)in components and the contents of active ingredient,and the content of these components all decreased with the fermentation time.The ability of scavenging ABTS free radicals[IC50=(0.29±0.02)g·L^(-1)]increased significantly compared with gastric digestion[(1.57±0.02)g·L^(-1)],and after 0.5 h of colonic fermentation,the ability also increased significantly.CONCLUSION Gastrointestinal digestion had a significant impact on the contents of active components in Epimedium,and the metabolism of these components mainly occurred in the colon.The intestinal digestion and colonic fermentation significantly improved the anti-ABTS activity of epimedium.展开更多
The conventional ethanol fermentaion is a typical inhibitory process, leading to low productivity and yield. A new ethanol fermentation process coupled with gas stripping and vacuum fash, named as strip-flash fermenta...The conventional ethanol fermentaion is a typical inhibitory process, leading to low productivity and yield. A new ethanol fermentation process coupled with gas stripping and vacuum fash, named as strip-flash fermentation, is proposed. The process is provided with the advantages of both stripping fermentation and flash fermentation, and improves the ethanol productivity by increasing the in-situ ethanol removal. And a model of flash-strip fermentation process was established, the results from the model were consistent with the experiment values. The theoretically analyses indicate that increasing gas flux and liquid phase recycling ratio can help to enhance productivity and yield of strip-flash fermentation process, and comparison to striping fermentation or flash fermentation, flash-strip fermentation has shown a better productivity. The results has also shown the possibilities of further application and optimization of this process.展开更多
Exopolysaccharides(EPSs)from lactic acid bacteria(LAB)play important roles in food,pharmaceutical,and medical applications.The aims of this study were to explore the digestive characteristics of Leuconostoc mesenteroi...Exopolysaccharides(EPSs)from lactic acid bacteria(LAB)play important roles in food,pharmaceutical,and medical applications.The aims of this study were to explore the digestive characteristics of Leuconostoc mesenteroides RSG7 EPS and its effects on the gut microbiota.RSG7 EPS could not be digested using salivary,gastric,or intestinal in vitro models of digestion.However,after fecal fermentation,the molecular weight(Mw)of RSG7 EPS decreased by 50.91±3.18%,and the surface morphology changed remarkably,developing wrinkles and dense fragments.These results indicated that RSG7 EPS could be degraded and consumed by human gut microbiota.Notably,in a fecal fermentation model,RSG7 EPS promoted the increase of beneficial bacteria,including Phascolarctobacterium with an abundance of 0.127±0.011 at 24 h and 0.149±0.021 at 48 h and Faecalicoccus with an abundance of 0.037±0.003 at 24 h and 0.016±0.003 at 48 h.In particular,the concentrations of propionic acid(24.176±0.978 mM),valeric acid(0.453±0.033 mM),and total short-chain fatty acids(57.378±2.027 mM,SCFAs)were significantly increased after 48 h in vitro fecal fermentation of RSG7 EPS.This study suggests that RSG7 EPS could be developed as a prebiotic to improve human intestinal health.展开更多
The introduction of the multi well plate miniaturisation technology with its associated automated dispensers, readers and integrated systems coupled with advances in life sciences has a propelling effect on the rate ...The introduction of the multi well plate miniaturisation technology with its associated automated dispensers, readers and integrated systems coupled with advances in life sciences has a propelling effect on the rate at which new potential drug molecules are discovered. The translation of these discoveries to real outcome now demands parallel approaches which allow large numbers of process options to be rapidly assessed. The engineering challenges in achieving this provide the motivation for the proposed work. In this work we used computational fluid dynamics(CFD) analysis to study flow conditions in a gas liquid contactor which has the potential to be used as a fermenter on a multi well format. The bioreactor had a working volume of 6 5 mL with the major dimensions equal to those of a single well of a 24 well plate. The 6 5 mL bioreactor was mechanically agitated and aerated by a single sparger placed beneath the bottom impeller. Detailed numerical procedure for solving the governing flow equations is given. The CFD results are combined with population balance equations to establish the size of the bubbles and their distribution in the bioreactor. Power curves with and without aeration are provided based on the simulated results.展开更多
Disruption of the intestinal epithelial barrier is a pivotal event of inflammatory bowel disease(IBD);however,effective therapeutic strategies to restore this barrier integrity remain elusive.In this study,we demonstr...Disruption of the intestinal epithelial barrier is a pivotal event of inflammatory bowel disease(IBD);however,effective therapeutic strategies to restore this barrier integrity remain elusive.In this study,we demonstrate that Cordyceps militaris(CM)modulates the gut microbiota by increasing the abundance of Megamonas and decreasing the Escherichia-Shigella genera,thereby promoting a microbiota profile conducive to gut health.Additionally,dihydroferulic acid(DHFA)was identified as a key microbiota metabolite derived from CM.Computational analyses revealed that protein kinase B(AKT1)serves as a direct binding target for DHFA in IBD.Cell-based experiments further demonstrated that pretreatment with DHFA reduces reactive oxygen species(ROS)levels and prevents AKT1 dysregulation,thus reducing hydrogen peroxide(H_(2)O_(2))-induced apoptosis in intestinal epithelial cells,ultimately alleviating epithelial injury.These results delineate the anti-inflammatory effects of CM and shed light on the development of strategies for the prevention and treatment of IBD.展开更多
基金financially supported by the National Natural Science Foundation of China(32000058)the Fundamental Research Funds for the Central Universities(JUSRP622003)。
文摘The prebiotic effects of hyaluronan(HA)are widely recognized,contributing to improved gut health and immune modulation.Despite its extensive use as dietary supplement,the specific interactions between HA oligosaccharides(o-HAs)and the gut microbiome remain largely unexplored.To investigate its role and metabolic fate in gut homeostasis,200 mg/day of o-HAs(average molecule weight 1 kDa)were added to an automated computer-controlled SIMulator of the Gastrointestinal tract(SIMGI).The results revealed a significant reshaping of the intestinal flora composition by o-HAs,notably reducing the Firmicutes/Bacteroides ratio.Fermentation of o-HAs by gut microbiota significantly increased the abundance of Bifidobacterium,Prevotellaceae_Prevotella,Dialister,Eubacterium,and Sutterella,but decreased that of Catenibacterium,Oscillospira,Klebsiella,and Citrobacter(P<0.05).This corresponded with significant enhancements in the content of short-chain fatty acids(SCFAs)such as acetic acid,propionic acid and n-butyric acid,highlighting the significant impact of o-HAs at the genus level.Furthermore,analysis of microbial function predicted the downregulation of pathological events in nine human diseases,particularly infectious ones(parasitic and bacterial).Potential inhibitions were observed in metabolic pathways associated with pentose and glucuronate interconversions as well as cationic antimicrobial peptide resistance.These findings underscore the in vitro prebiotic effects of o-HAs and their potential relevance in managing diverticular diseases or preventing metabolic disorders through the regulation of gut microbiota.
文摘To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.
基金Shandong Province Department of Education Science and Technology Program(No.J05C11)
文摘Beer fermentation process is a complex biochemical reaction process.It is the most important to control temperature of the wort in fermentation tank in accordance with the beer fermentation temperature curve so as to ensure the completion of fermentation.The controlled object is characterized by large inertia,long time delay and mutual coupling of three temperature areas.Based on this,a temperature control method for beer fermentation system is designed.Using digital incremental proportion integration differentiation （PID） control algorithm,the controlled quantity is transmitted to the controlled object after diagonal matrix decoupling.This simulation system can be completed in laboratory using VB and Kingview software,so it has the features of good security and low cost.It is very suitable for experimental teaching.
文摘The feasibility of ethanol separation from ethanol-water solution saturated with glucose by gas strippingis showil theoretically and experimentally. The effects of glucose on the saturated vapor pressure of ethanol, and thatof operating temperature and gas stripping flow rates, K/G, on Stripping result are discussed.
基金Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas,China(SLGPT2019KF04-04)and the ERDF through the COMPETE2020-Programa Operacional Competitividade e Internacionalização(POCI),Portugal。
文摘OBJECTIVE Epimedium is rich in a variety of beneficial active ingredients,and has been widely used in the ethnopharmacological practices,however,its biotransformation in gastrointestinal digestions remain unclear.This study aimed to investigate the dynamic changes of components and biological activity of Epimedium in the in vitro simulated digestion and subsequent human faecal fermentation.METHODS The models of in vitro simulated saliva,gastric and intestinal digestion,as well as colonic fermentation were constructed to simulate the digestion process of Epimedium.The dynamic changes of components of Epimedium during the simulated digestions in vitro and subsequent human faecal fermentation were investigated by UPLC-MS,HPLC-DAD combined with principal component analysis(PCA)and multi-ingredient quantitative analysis.RESULTS A variety of metabolites with high contents were produced after 0.5 h of intestinal digestion and colonic fermentation 0.5 h.Application of PCA to HPLC data showed the obvious separation of colonic fermentation 0.5 h stage samples from other colonic fermentation stages samples(24,48 and 72 h).Additionally,non-digestion and saliva digestion stage samples clustered together,and there was obvious separation between intestinal digestion samples and gastric digestion samples.The contents of epimedium C,icariin and baohuside I all increased significantly after intestinal digestion[58.70±7.08,47.15±5.68 and(12.78±0.55)mg·g^(-1)]compared with gastric digestion[29.00±5.65,17.40±4.55 and(2.77±0.19)mg·g^(-1)].There were significant differences between sample after 0.5 h of colonic fermentation[64.22±9.32,51.26±6.33 and(16.68±3.19)mg·g^(-1)]and other time points(24,48 and 72 h)in components and the contents of active ingredient,and the content of these components all decreased with the fermentation time.The ability of scavenging ABTS free radicals[IC50=(0.29±0.02)g·L^(-1)]increased significantly compared with gastric digestion[(1.57±0.02)g·L^(-1)],and after 0.5 h of colonic fermentation,the ability also increased significantly.CONCLUSION Gastrointestinal digestion had a significant impact on the contents of active components in Epimedium,and the metabolism of these components mainly occurred in the colon.The intestinal digestion and colonic fermentation significantly improved the anti-ABTS activity of epimedium.
文摘The conventional ethanol fermentaion is a typical inhibitory process, leading to low productivity and yield. A new ethanol fermentation process coupled with gas stripping and vacuum fash, named as strip-flash fermentation, is proposed. The process is provided with the advantages of both stripping fermentation and flash fermentation, and improves the ethanol productivity by increasing the in-situ ethanol removal. And a model of flash-strip fermentation process was established, the results from the model were consistent with the experiment values. The theoretically analyses indicate that increasing gas flux and liquid phase recycling ratio can help to enhance productivity and yield of strip-flash fermentation process, and comparison to striping fermentation or flash fermentation, flash-strip fermentation has shown a better productivity. The results has also shown the possibilities of further application and optimization of this process.
基金supported by the National Natural Science Foundation of China(grant number 32101923)the Basic Research Program of Shanxi Province(grant number 20210302124066)+1 种基金Research award fund for the outstanding doctor of the Department of Finance of Shanxi Province,China(grant numbers 0110/02010008,0503/02010189)the Doctoral initial fund of Shanxi Normal University(grant numbers 0505/02070532,0505/02070485).
文摘Exopolysaccharides(EPSs)from lactic acid bacteria(LAB)play important roles in food,pharmaceutical,and medical applications.The aims of this study were to explore the digestive characteristics of Leuconostoc mesenteroides RSG7 EPS and its effects on the gut microbiota.RSG7 EPS could not be digested using salivary,gastric,or intestinal in vitro models of digestion.However,after fecal fermentation,the molecular weight(Mw)of RSG7 EPS decreased by 50.91±3.18%,and the surface morphology changed remarkably,developing wrinkles and dense fragments.These results indicated that RSG7 EPS could be degraded and consumed by human gut microbiota.Notably,in a fecal fermentation model,RSG7 EPS promoted the increase of beneficial bacteria,including Phascolarctobacterium with an abundance of 0.127±0.011 at 24 h and 0.149±0.021 at 48 h and Faecalicoccus with an abundance of 0.037±0.003 at 24 h and 0.016±0.003 at 48 h.In particular,the concentrations of propionic acid(24.176±0.978 mM),valeric acid(0.453±0.033 mM),and total short-chain fatty acids(57.378±2.027 mM,SCFAs)were significantly increased after 48 h in vitro fecal fermentation of RSG7 EPS.This study suggests that RSG7 EPS could be developed as a prebiotic to improve human intestinal health.
文摘The introduction of the multi well plate miniaturisation technology with its associated automated dispensers, readers and integrated systems coupled with advances in life sciences has a propelling effect on the rate at which new potential drug molecules are discovered. The translation of these discoveries to real outcome now demands parallel approaches which allow large numbers of process options to be rapidly assessed. The engineering challenges in achieving this provide the motivation for the proposed work. In this work we used computational fluid dynamics(CFD) analysis to study flow conditions in a gas liquid contactor which has the potential to be used as a fermenter on a multi well format. The bioreactor had a working volume of 6 5 mL with the major dimensions equal to those of a single well of a 24 well plate. The 6 5 mL bioreactor was mechanically agitated and aerated by a single sparger placed beneath the bottom impeller. Detailed numerical procedure for solving the governing flow equations is given. The CFD results are combined with population balance equations to establish the size of the bubbles and their distribution in the bioreactor. Power curves with and without aeration are provided based on the simulated results.
基金supported by the National Key Research and Development Program for Food Nutrition and Safety(2023YFF1104100)State Key Laboratory of Applied Microbiology Southern China(SKLAM002-2022)Guangdong Provincial Key Laboratory(2020B121201009).
文摘Disruption of the intestinal epithelial barrier is a pivotal event of inflammatory bowel disease(IBD);however,effective therapeutic strategies to restore this barrier integrity remain elusive.In this study,we demonstrate that Cordyceps militaris(CM)modulates the gut microbiota by increasing the abundance of Megamonas and decreasing the Escherichia-Shigella genera,thereby promoting a microbiota profile conducive to gut health.Additionally,dihydroferulic acid(DHFA)was identified as a key microbiota metabolite derived from CM.Computational analyses revealed that protein kinase B(AKT1)serves as a direct binding target for DHFA in IBD.Cell-based experiments further demonstrated that pretreatment with DHFA reduces reactive oxygen species(ROS)levels and prevents AKT1 dysregulation,thus reducing hydrogen peroxide(H_(2)O_(2))-induced apoptosis in intestinal epithelial cells,ultimately alleviating epithelial injury.These results delineate the anti-inflammatory effects of CM and shed light on the development of strategies for the prevention and treatment of IBD.
