Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vi...Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vironment and intensify carbon emissions.However,the use of microbially induced calcium carbonate pre-cipitation(MICP)to obtain bio-cement is a novel technique with the potential to induce soil stability,providing a low-carbon,environment-friendly,and sustainable integrated solution for some geotechnical engineering pro-blems in the environment.This paper presents a comprehensive review of the latest progress in soil improvement based on the MICP strategy.It systematically summarizes and overviews the mineralization mechanism,influ-encing factors,improved methods,engineering characteristics,and current field application status of the MICP.Additionally,it also explores the limitations and correspondingly proposes prospective applications via the MICP approach for soil improvement.This review indicates that the utilization of different environmental calcium-based wastes in MICP and combination of materials and MICP are conducive to meeting engineering and market demand.Furthermore,we recommend and encourage global collaborative study and practice with a view to commercializing MICP technique in the future.The current review purports to provide insights for engineers and interdisciplinary researchers,and guidance for future engineering applications.展开更多
[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the charac...[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.展开更多
To evaluate the water storage and project the future evolution of glaciers, the ice-thickness of glaciers is an essential input. However, direct measurements of ice thickness are labo- rious, not feasible everywhere, ...To evaluate the water storage and project the future evolution of glaciers, the ice-thickness of glaciers is an essential input. However, direct measurements of ice thickness are labo- rious, not feasible everywhere, and necessarily restricted to a small number of glaciers. In this article, we develop a simple method to estimate the ice-thickness along flow-line of mountain glaciers. Different from the traditional method based on shallow ice approximation （SIA）, which gives a relationship be- tween ice thickness, surface slope, and yield stress of glaciers, the improved method considers and pre- sents a simple way to calibrate the influence of valley wall on ice discharge. The required inputs are the glacier surface topography and outlines. This shows the potential of the method for estimating the ice-thickness distribution and volume of glaciers without using of direct thickness measurements.展开更多
A variational method is developed to retrieve winds in the first step and then thermodynamic fields in the second step from Doppler radar observations. In the first step, wind fields are retrieved at two time levels: ...A variational method is developed to retrieve winds in the first step and then thermodynamic fields in the second step from Doppler radar observations. In the first step, wind fields are retrieved at two time levels: the beginning and ending times of the data assimilation period, simultaneously from two successive volume scans by using the weak form constraints provided by the mass continuity and vorticity equations. As the retrieved wind fields are expressed by Legendre polynomial expansions at the beginning and ending times, the time tendency term in the vorticity equation can be conveniently formulated, and the retrieved winds can be compared with the radar observed radial winds in the cost function at the precise time and position of each radar beam. In the second step, the perturbation pressure and temperature fields at the middle time are then derived from the retrieved wind fields and the velocity time tendency by using the weak form constraints provided by the three momentum equations. The merits of the new method are demonstrated by numerical experiments with simulated radar observations and compared with the traditional least squares methods which consider neither the precise observation times and positions nor the velocity time tendency. The new method is also applied to real radar data for a heavy rainfall event during the 2001 Meiyu season in China.展开更多
In this paper, we report an improved method for preparing 2--alkoxy 5 fluoro--3H --4--pyrimidone from 2--chloro--5--fluoro--3H--4--pyrimidone and sodium alkoxide under normal pressure at reflux temperature. Some new c...In this paper, we report an improved method for preparing 2--alkoxy 5 fluoro--3H --4--pyrimidone from 2--chloro--5--fluoro--3H--4--pyrimidone and sodium alkoxide under normal pressure at reflux temperature. Some new compounds were synthesized in higher yield by this method.展开更多
A simple stepwise folding process has been developed to simulate RNA secondary structure formation.Modifications for the energy parameters of various loops were included in the program.Five possible types of pseudokno...A simple stepwise folding process has been developed to simulate RNA secondary structure formation.Modifications for the energy parameters of various loops were included in the program.Five possible types of pseudoknots including the well known H-type pseudoknot were permitted to occur if reasonable.We have applied this approach to e number of RNA sequences.The prediction accuracies we obtained were higher than those in published papers.展开更多
Dear Editor, In recent years, post-translational modifications （PTMs） by small ubiquitin-related modifiers （SUMOs） have emerged as an important regulatory mechanism for both cellular and viral processes （Ribet an...Dear Editor, In recent years, post-translational modifications （PTMs） by small ubiquitin-related modifiers （SUMOs） have emerged as an important regulatory mechanism for both cellular and viral processes （Ribet and Cossart, 2010）. Identifying the SUMOylation sites of the target protein is important to understand the molecular mechanism under- lying SUMO modification and virus-host interactions, as well as provide new insights into antiviral drug develop- ment （Wimmer and Schreiner, 2015）. Traditional site- directed mutagenesis for identifying viral protein SUMO- ylation sites lacks a specific aim and is laborious （McManus et al., 2016）. Recently, mass spectrometry （MS） has been employed as an accurate and sensitive tool to identify PTM sites, thereby greatly expanding the number of known SUMOylated proteins （Pedrioli et al., 2006）. However, during viral infection, SUMOylation is highly dynamic and SUMOylated viral proteins often have low abundance, which makes studying SUMOylation under natural conditions difficult.展开更多
The variation of air temperature measurement errors using two different radiation shields （DTR502B Vaisala,Finland,and HYTFZ01,Huayun Tongda Satcom,China） was studied.Datasets were collected in the field at the Daxi...The variation of air temperature measurement errors using two different radiation shields （DTR502B Vaisala,Finland,and HYTFZ01,Huayun Tongda Satcom,China） was studied.Datasets were collected in the field at the Daxing weather station in Beijing from June 2011 to May 2012.Most air temperature values obtained with these two commonly used radiation shields were lower than the reference records obtained with the new Fiber Reinforced Polymers （FRP） Stevenson screen.In most cases,the air temperature errors when using the two devices were smaller on overcast and rainy days than on sunny days; and smaller when using the imported rather than the Chinese shield.The measured errors changed sharply at sunrise and sunset,and reached maxima at noon.Their diurnal variation characteristics were,naturally,related to changes in solar radiation.The relationships between the record errors,global radiation,and wind speed were nonlinear.An improved correction method was proposed based on the approach described by Nakamura and Mahrt （2005） （NM05）,in which the impact of the solar zenith angle （SZA） on the temperature error is considered and extreme errors due to changes in SZA can be corrected effectively.Measurement errors were reduced significantly after correction by either method for both shields.The error reduction rate using the improved correction method for the Chinese and imported shields were 3.3％ and 40.4％ higher than those using the NM05 method,respectively.展开更多
Sponge city(SPC)is proposed to solve the issues such as the degradation of urban water ecosystem environment,imbalanced water resource allocation,urban water logging,and water contamination.The PPP(Public Private Part...Sponge city(SPC)is proposed to solve the issues such as the degradation of urban water ecosystem environment,imbalanced water resource allocation,urban water logging,and water contamination.The PPP(Public Private Partnership)model is combined to release the government pressure of SPC project construction.The development of the SPC-PPP model makes significant contributions to the sustainable development and the enhancement of urban resilience against water-related disasters.However,there is no scientific performance evaluation system on its operation period has been conducted.Therefore,the SPC-PPP Evaluation model aims to objectively and reasonably assess project effectiveness,promote its development and refine the evaluation framework.This paper has set up the MEE model for performance evaluation,with improved Matter-Element Extension method to assign values to the evaluation indices.The research results show that:(1)The MEE model is more accurate in the performance evaluation and its effectiveness is reflected in its ability to capture the correlation among different indices in the same membership,rather than merely focusing on individual indices.(2)The proposed approach provided a new aspect for performance evaluation,improving the accuracy of evaluation and promoting the development of SPC-PPP project.展开更多
In this paper,we develop an advanced computational framework for the topology optimization of orthotropic materials using meshless methods.The approximation function is established based on the improved moving least s...In this paper,we develop an advanced computational framework for the topology optimization of orthotropic materials using meshless methods.The approximation function is established based on the improved moving least squares(IMLS)method,which enhances the efficiency and stability of the numerical solution.The numerical solution formulas are derived using the improved element-free Galerkin(IEFG)method.We introduce the solid isotropic microstructures with penalization(SIMP)model to formulate a mathematical model for topology opti-mization,which effectively penalizes intermediate densities.The optimization problem is defined with the numerical solution formula and volume fraction as constraints.The objective function,which is the minimum value of flexibility,is optimized iteratively using the optimization criterion method to update the design variables efficiently and converge to an optimal solution.Sensitivity analysis is performed using the adjoint method,which provides accurate and efficient gradient information for the optimization algorithm.We validate the proposed framework through a series of numerical examples,including clamped beam,cantilever beam,and simply supported beam made of orthotropic materials.The convergence of the objective function is demonstrated by increasing the number of iterations.Additionally,the stability of the iterative process is analyzed by examining the fluctuation law of the volume fraction.By adjusting the parameters to an appropriate range,we achieve the final optimization results of the IEFG method without the checkerboard phenomenon.Comparative studies between the Element-Free Galerkin(EFG)and IEFG methods reveal that both methods yield consistent optimization results under identical parameter settings.However,the IEFG method significantly reduces computational time,highlighting its efficiency and suitability for orthotropic materials.展开更多
The stability of the tunnel portal slope is crucial for ensuring safe tunnel construction.Thus,a sound stability evaluation is of significance.Given the unique geological characteristics of tunnel portal slopes,it is ...The stability of the tunnel portal slope is crucial for ensuring safe tunnel construction.Thus,a sound stability evaluation is of significance.Given the unique geological characteristics of tunnel portal slopes,it is necessary to establish a specific evaluation indicator system that differs from those used for ordinary slopes.Based on the unascertained measure method,uncertainties in the indicator are addressed by introducing the left and right half cloud asymmetric cloud model to optimize the linear membership function.The subjectivity of confidence criterion level identification is also improved by using the Euclidean distance method.Thus,a stability evaluation model for the tunnel portal slope is established based on the improved unascertained measure method.Finally,using the collected tunnel portal slope data,the results of four evaluation methods are compared with the safety factor levels.The evaluation methods include the traditional unascertained measure method,the method improved by using the left and right half cloud asymmetric cloud model,the method improved by using the Euclidean distance method,and the method improved by using both the left and right half cloud asymmetric cloud model and the Euclidean distance method.The results show that the accuracy rates of these four methods are 50%,55%,85%,and 90%,respectively.Among them,the joint improvement method has the slightest deviation,with only one level,while the other three methods had deviations of two levels.This result verifies the stability and effectiveness of the joint improvement method,providing a reference for tunnel portal slope stability evaluation.展开更多
The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experi...The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experimental analysis of the evolution process of the short-circuit arc to the secondary arc is critical.In this study,an improved charge simulation method was used to develop the internal-space electric-field model of the short-circuit arc.The intensity of the electric field was used as an independent variable to describe the initial shape of the secondary arc.A secondary arc evolution model was developed based on this model.Moreover,the accuracy of the model was evaluated by comparison with physical experimental results.When the secondary arc current increased,the arcing time and dispersion increased.There is an overall trend of increasing arc length with increasing arcing time.Nevertheless,there is a reduction in arc length during arc ignition due to short circuits between the arc columns.Furthermore,the arcing time decreased in the range of 0°-90°as the angle between the wind direction and the x-axis increased.This work investigated the method by which short-circuit arcs evolve into secondary arcs.The results can be used to develop the secondary arc evolution model and to provide both a technical and theoretical basis for secondary arc suppression.展开更多
This study proposes an effective method to enhance the accuracy of the Differential Quadrature Method(DQM)for calculating the dynamic characteristics of functionally graded beams by improving the form of discrete node...This study proposes an effective method to enhance the accuracy of the Differential Quadrature Method(DQM)for calculating the dynamic characteristics of functionally graded beams by improving the form of discrete node distribution.Firstly,based on the first-order shear deformation theory,the governing equation of free vibration of a functionally graded beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam axial displacement,transverse displacement,and cross-sectional rotation angle by considering the effects of shear deformation and rotational inertia of the beam cross-section.Then,ignoring the shear deformation of the beam section and only considering the effect of the rotational inertia of the section,the governing equation of the beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam transverse displacement.Based on the differential quadrature method theory,the eigenvalue problem of ordinary differential equations is transformed into the eigenvalue problem of standard generalized algebraic equations.Finally,the first several natural frequencies of the beam can be calculated.The feasibility and accuracy of the improved DQM are verified using the finite element method(FEM)and combined with the results of relevant literature.展开更多
[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the ...[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...展开更多
Derivation of equivalent current systems(ECS)from a global magnetospheric magnetohydrodynamics(MHD)model is very useful in studying magnetosphere-ionosphere coupling,ground induction effects,and space weather forecast...Derivation of equivalent current systems(ECS)from a global magnetospheric magnetohydrodynamics(MHD)model is very useful in studying magnetosphere-ionosphere coupling,ground induction effects,and space weather forecast.In this study we introduce an improved method to derive the ECS from a global MHD model,which takes account of the obliqueness of the magnetic field lines.By comparing the ECS derived from this improved method and the previous method,we find that the main characteristics of the ECS derived from the two methods are generally consistent with each other,but the eastward-westward component of the geomagnetic perturbation calculated from the ECS derived from the improved method is much stronger than that from the previous method.We then compare the geomagnetic perturbation as a function of the interplanetary magnetic field(IMF)clock angle calculated from the ECS derived from both methods with the observations.The comparison indicates that the improved method can improve the performance of the simulation.Furthermore,it is found that the incomplete counterbalance of the geomagnetic effect produced by the ionospheric poloidal current and field-aligned current(FAC)contributes to most of the eastward-westward component of geomagnetic perturbation.展开更多
An improved method of angle measurement is proposed based on a parallel plate interferometer. A position detection system is incorporated into a parallel plate interferometer in order to realize large deflection angle...An improved method of angle measurement is proposed based on a parallel plate interferometer. A position detection system is incorporated into a parallel plate interferometer in order to realize large deflection angle measurement. A reflecting mirror is introduced for increasing the measurement resolution. In experiments, a deflection angle of a measured target was measured within ～3° with high accuracy. And as a phase modulating interferometer, it was used to measure a small angular displacement with a repeatability of 5.5 × 10^-8 rad.展开更多
This study successfully deals with the inhomogeneous dimension problem of load separation assumption, which is the theoretical basis of the normalization method. According to the dimensionless load separation principl...This study successfully deals with the inhomogeneous dimension problem of load separation assumption, which is the theoretical basis of the normalization method. According to the dimensionless load separation principle, the normalization method has been improved by intro- ducing a forcible blunting correction. With the improved normalization method, the J-resistance curves of five different metallic materials of CT and SEB specimens are estimated. The forcible blunting correction of initial crack size plays an important role in the J-resistance curve estima- tion, which is closely related to the strain hardening level of material. The higher level of strain hardening leads to a greater difference in JQ determined by different slopes of the blunting line. If the blunting line coefficient recommended by ASTM E1820-11 is used in the improved nor- realization method, it will lead to greater fracture resistance than that processed by the blunting line coefficient recommended by ISO 12135-2002. Therefore, the influence of the blunting line on the determination of JQ must be taken into full account in the fracture toughness assessment of metallic materials.展开更多
In this paper, an improved interpolating moving least-square （IIMLS） method is presented. The shape function of the IIMLS method satisfies the property of the Kronecker 5 function. The weight function used in the II...In this paper, an improved interpolating moving least-square （IIMLS） method is presented. The shape function of the IIMLS method satisfies the property of the Kronecker 5 function. The weight function used in the IIMLS method is nonsingular. Then the IIMLS method can overcome the difficulties caused by the singularity of the weight function in the IMLS method. The number of unknown coefficients in the trial function of the IIMLS method is less than that of the moving least-square （MLS） approximation. Then by combining the IIMLS method with the Galerkin weak form of the potential problem, the improved interpolating element-free Galerkin （IIEFG） method for two-dimensional potential problems is presented. Compared with the conventional element-free Galerkin （EFG） method, the IIEFG method can directly use the essential boundary conditions. Then the IIEFG method has higher accuracy. For demonstration, three numerical examples are solved using the IIEFG method.展开更多
For natural water, method of water quality evaluation based on improved fuzzy matter-element evaluation method is presented. Two important parts are improved, the weights determining and fuzzy membership functions. Th...For natural water, method of water quality evaluation based on improved fuzzy matter-element evaluation method is presented. Two important parts are improved, the weights determining and fuzzy membership functions. The coefficient of variation of each indicator is used to determine the weight instead of traditional calculating superscales method. On the other hand, fuzzy matter-elements are constructed, and normal membership degrees are used instead of traditional trapezoidal ones. The composite fuzzy matter-elements with associated coefficient are constructed through associated transformation. The levels of natural water quality are determined according to the principle of maximum correlation. The improved fuzzy matter-element evaluation method is applied to evaluate water quality of the Luokou mainstream estuary at the first ten weeks in 2011 with the coefficient of variatiola method determining the weights. Water quality of Luokou mainstream estuary is dropping from level I to level II. The results of the improved evaluation method are basically the same as the official water quality. The variation coefficient method can reduce the workload, and overcome the adverse effects from abnormal values, compared with the traditional calculating superscales method. The results of improved fuzzy matter- element evaluation method are more credible than the ones of the traditional evaluation method. The improved evaluation method can use information of monitoring data more scientifically and comprehensively, and broaden a new evaluation method for water quality assessment.展开更多
The relative importance of each pollution factor in analytical hierarchy process (AHP) method comes from pooling expert opinions in general. Because expert opinions are based on information and judgment criteria, dete...The relative importance of each pollution factor in analytical hierarchy process (AHP) method comes from pooling expert opinions in general. Because expert opinions are based on information and judgment criteria, determining their weight may lead to uncertainty.. Therefore, an improved AHP method had been developed. The process of the improved AHP method involved four key procedures. The weights of pollution factors were completely related to the objective monitoring data through the standardization of these procedures. The environmental comprehensive quality of water and sediment of Xuanwu Lake, Nanjing, China had been evaluated. The environmental quality comprehensive indices (EQCI) of the water in 1991-1995 and 1996-2000 were 3.32 and 1.85, respectively, indicating that the water quality improved. The EQCI of the sediment in 1995 and 2000 indicates that the sediment contamination decreased from 1995 to 2000. Such results agreed with the fact that the lake had been under comprehensive control. However, with the classical AHP method, the EQCI of the sediment in the northwestern part of the lake may have indicated that sediment contamination increased from 1995 to 2000. The discrepancy may have resulted from the judgment difference of the experts. The improved AHP method can avoid arbitrariness of subjective judgment and can reflect the real influential factors of environment pollution in different periods or regions.展开更多
基金funded by the National Natural Science Foundation of China(No.41962016)the Natural Science Foundation of NingXia(Nos.2023AAC02023,2023A1218,and 2021AAC02006).
文摘Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vironment and intensify carbon emissions.However,the use of microbially induced calcium carbonate pre-cipitation(MICP)to obtain bio-cement is a novel technique with the potential to induce soil stability,providing a low-carbon,environment-friendly,and sustainable integrated solution for some geotechnical engineering pro-blems in the environment.This paper presents a comprehensive review of the latest progress in soil improvement based on the MICP strategy.It systematically summarizes and overviews the mineralization mechanism,influ-encing factors,improved methods,engineering characteristics,and current field application status of the MICP.Additionally,it also explores the limitations and correspondingly proposes prospective applications via the MICP approach for soil improvement.This review indicates that the utilization of different environmental calcium-based wastes in MICP and combination of materials and MICP are conducive to meeting engineering and market demand.Furthermore,we recommend and encourage global collaborative study and practice with a view to commercializing MICP technique in the future.The current review purports to provide insights for engineers and interdisciplinary researchers,and guidance for future engineering applications.
基金Supported by the National Natural Science Foundation of China(31060152)the Natural Science Foundation of the Xinjiang Uygur Autonomous Region,China(2010211A02)the Key Program for Animal Sciences of Xinjiang University,China~~
文摘[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.
基金supported by the National Basic Research Program of China (No. 2007CB411501)the Knowledge Innovation Project of the Chinese Academy of Sciences (No. KZCX2-EW-311)+1 种基金the National Natural Science Foundation of China (Nos. 91025012, J0930003/J0109)the Project for Outstanding Young Scientists of the National Natural Science Foundation of China (No. 40121101)
文摘To evaluate the water storage and project the future evolution of glaciers, the ice-thickness of glaciers is an essential input. However, direct measurements of ice thickness are labo- rious, not feasible everywhere, and necessarily restricted to a small number of glaciers. In this article, we develop a simple method to estimate the ice-thickness along flow-line of mountain glaciers. Different from the traditional method based on shallow ice approximation （SIA）, which gives a relationship be- tween ice thickness, surface slope, and yield stress of glaciers, the improved method considers and pre- sents a simple way to calibrate the influence of valley wall on ice discharge. The required inputs are the glacier surface topography and outlines. This shows the potential of the method for estimating the ice-thickness distribution and volume of glaciers without using of direct thickness measurements.
文摘A variational method is developed to retrieve winds in the first step and then thermodynamic fields in the second step from Doppler radar observations. In the first step, wind fields are retrieved at two time levels: the beginning and ending times of the data assimilation period, simultaneously from two successive volume scans by using the weak form constraints provided by the mass continuity and vorticity equations. As the retrieved wind fields are expressed by Legendre polynomial expansions at the beginning and ending times, the time tendency term in the vorticity equation can be conveniently formulated, and the retrieved winds can be compared with the radar observed radial winds in the cost function at the precise time and position of each radar beam. In the second step, the perturbation pressure and temperature fields at the middle time are then derived from the retrieved wind fields and the velocity time tendency by using the weak form constraints provided by the three momentum equations. The merits of the new method are demonstrated by numerical experiments with simulated radar observations and compared with the traditional least squares methods which consider neither the precise observation times and positions nor the velocity time tendency. The new method is also applied to real radar data for a heavy rainfall event during the 2001 Meiyu season in China.
基金The project is supported by Natural Science Foundation of Shandong.
文摘In this paper, we report an improved method for preparing 2--alkoxy 5 fluoro--3H --4--pyrimidone from 2--chloro--5--fluoro--3H--4--pyrimidone and sodium alkoxide under normal pressure at reflux temperature. Some new compounds were synthesized in higher yield by this method.
文摘A simple stepwise folding process has been developed to simulate RNA secondary structure formation.Modifications for the energy parameters of various loops were included in the program.Five possible types of pseudoknots including the well known H-type pseudoknot were permitted to occur if reasonable.We have applied this approach to e number of RNA sequences.The prediction accuracies we obtained were higher than those in published papers.
文摘Dear Editor, In recent years, post-translational modifications （PTMs） by small ubiquitin-related modifiers （SUMOs） have emerged as an important regulatory mechanism for both cellular and viral processes （Ribet and Cossart, 2010）. Identifying the SUMOylation sites of the target protein is important to understand the molecular mechanism under- lying SUMO modification and virus-host interactions, as well as provide new insights into antiviral drug develop- ment （Wimmer and Schreiner, 2015）. Traditional site- directed mutagenesis for identifying viral protein SUMO- ylation sites lacks a specific aim and is laborious （McManus et al., 2016）. Recently, mass spectrometry （MS） has been employed as an accurate and sensitive tool to identify PTM sites, thereby greatly expanding the number of known SUMOylated proteins （Pedrioli et al., 2006）. However, during viral infection, SUMOylation is highly dynamic and SUMOylated viral proteins often have low abundance, which makes studying SUMOylation under natural conditions difficult.
基金financially supported by the Meteorological Key Technology Integration and Application Project funded by the China Meteorological Administration (Grant No.CAMGJ2012M01)the Special Fund of Beijing Meteorological Bureau (Grant No.2011BMBKYZX04)the Nation Natural Science Foundation of China (Grant No.41275114)
文摘The variation of air temperature measurement errors using two different radiation shields （DTR502B Vaisala,Finland,and HYTFZ01,Huayun Tongda Satcom,China） was studied.Datasets were collected in the field at the Daxing weather station in Beijing from June 2011 to May 2012.Most air temperature values obtained with these two commonly used radiation shields were lower than the reference records obtained with the new Fiber Reinforced Polymers （FRP） Stevenson screen.In most cases,the air temperature errors when using the two devices were smaller on overcast and rainy days than on sunny days; and smaller when using the imported rather than the Chinese shield.The measured errors changed sharply at sunrise and sunset,and reached maxima at noon.Their diurnal variation characteristics were,naturally,related to changes in solar radiation.The relationships between the record errors,global radiation,and wind speed were nonlinear.An improved correction method was proposed based on the approach described by Nakamura and Mahrt （2005） （NM05）,in which the impact of the solar zenith angle （SZA） on the temperature error is considered and extreme errors due to changes in SZA can be corrected effectively.Measurement errors were reduced significantly after correction by either method for both shields.The error reduction rate using the improved correction method for the Chinese and imported shields were 3.3％ and 40.4％ higher than those using the NM05 method,respectively.
基金supported by the Open Fund of Hubei Key Laboratory of Construction Management in Hydropower Engineering(Grant No.2016KSD04)the Open Fund of Engineering Research Center of Eco-environment in Three Gorges Reservoir Region,Ministry of Education(Grant No.KF2016-11).
文摘Sponge city(SPC)is proposed to solve the issues such as the degradation of urban water ecosystem environment,imbalanced water resource allocation,urban water logging,and water contamination.The PPP(Public Private Partnership)model is combined to release the government pressure of SPC project construction.The development of the SPC-PPP model makes significant contributions to the sustainable development and the enhancement of urban resilience against water-related disasters.However,there is no scientific performance evaluation system on its operation period has been conducted.Therefore,the SPC-PPP Evaluation model aims to objectively and reasonably assess project effectiveness,promote its development and refine the evaluation framework.This paper has set up the MEE model for performance evaluation,with improved Matter-Element Extension method to assign values to the evaluation indices.The research results show that:(1)The MEE model is more accurate in the performance evaluation and its effectiveness is reflected in its ability to capture the correlation among different indices in the same membership,rather than merely focusing on individual indices.(2)The proposed approach provided a new aspect for performance evaluation,improving the accuracy of evaluation and promoting the development of SPC-PPP project.
基金supported by the Graduate Student Scientific Research Innovation Project through Research Innovation Fund for Graduate Students in Shanxi Province(Project No.2024KY648).
文摘In this paper,we develop an advanced computational framework for the topology optimization of orthotropic materials using meshless methods.The approximation function is established based on the improved moving least squares(IMLS)method,which enhances the efficiency and stability of the numerical solution.The numerical solution formulas are derived using the improved element-free Galerkin(IEFG)method.We introduce the solid isotropic microstructures with penalization(SIMP)model to formulate a mathematical model for topology opti-mization,which effectively penalizes intermediate densities.The optimization problem is defined with the numerical solution formula and volume fraction as constraints.The objective function,which is the minimum value of flexibility,is optimized iteratively using the optimization criterion method to update the design variables efficiently and converge to an optimal solution.Sensitivity analysis is performed using the adjoint method,which provides accurate and efficient gradient information for the optimization algorithm.We validate the proposed framework through a series of numerical examples,including clamped beam,cantilever beam,and simply supported beam made of orthotropic materials.The convergence of the objective function is demonstrated by increasing the number of iterations.Additionally,the stability of the iterative process is analyzed by examining the fluctuation law of the volume fraction.By adjusting the parameters to an appropriate range,we achieve the final optimization results of the IEFG method without the checkerboard phenomenon.Comparative studies between the Element-Free Galerkin(EFG)and IEFG methods reveal that both methods yield consistent optimization results under identical parameter settings.However,the IEFG method significantly reduces computational time,highlighting its efficiency and suitability for orthotropic materials.
基金supported by the National Natural Science Foundation of China(Grant No.42377191,42072300)“The 14th Five Year Plan”Hubei Provincial advantaged characteristic disciplines(groups)project of Wuhan University of Science and Technology(Grant No.2023A0303).
文摘The stability of the tunnel portal slope is crucial for ensuring safe tunnel construction.Thus,a sound stability evaluation is of significance.Given the unique geological characteristics of tunnel portal slopes,it is necessary to establish a specific evaluation indicator system that differs from those used for ordinary slopes.Based on the unascertained measure method,uncertainties in the indicator are addressed by introducing the left and right half cloud asymmetric cloud model to optimize the linear membership function.The subjectivity of confidence criterion level identification is also improved by using the Euclidean distance method.Thus,a stability evaluation model for the tunnel portal slope is established based on the improved unascertained measure method.Finally,using the collected tunnel portal slope data,the results of four evaluation methods are compared with the safety factor levels.The evaluation methods include the traditional unascertained measure method,the method improved by using the left and right half cloud asymmetric cloud model,the method improved by using the Euclidean distance method,and the method improved by using both the left and right half cloud asymmetric cloud model and the Euclidean distance method.The results show that the accuracy rates of these four methods are 50%,55%,85%,and 90%,respectively.Among them,the joint improvement method has the slightest deviation,with only one level,while the other three methods had deviations of two levels.This result verifies the stability and effectiveness of the joint improvement method,providing a reference for tunnel portal slope stability evaluation.
基金supported by National Natural Science Foundation of China(Nos.92066108 and 51277061)。
文摘The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experimental analysis of the evolution process of the short-circuit arc to the secondary arc is critical.In this study,an improved charge simulation method was used to develop the internal-space electric-field model of the short-circuit arc.The intensity of the electric field was used as an independent variable to describe the initial shape of the secondary arc.A secondary arc evolution model was developed based on this model.Moreover,the accuracy of the model was evaluated by comparison with physical experimental results.When the secondary arc current increased,the arcing time and dispersion increased.There is an overall trend of increasing arc length with increasing arcing time.Nevertheless,there is a reduction in arc length during arc ignition due to short circuits between the arc columns.Furthermore,the arcing time decreased in the range of 0°-90°as the angle between the wind direction and the x-axis increased.This work investigated the method by which short-circuit arcs evolve into secondary arcs.The results can be used to develop the secondary arc evolution model and to provide both a technical and theoretical basis for secondary arc suppression.
基金Anhui Provincial Natural Science Foundation(2308085QD124)Anhui Province University Natural Science Research Project(GrantNo.2023AH050918)The University Outstanding Youth Talent Support Program of Anhui Province.
文摘This study proposes an effective method to enhance the accuracy of the Differential Quadrature Method(DQM)for calculating the dynamic characteristics of functionally graded beams by improving the form of discrete node distribution.Firstly,based on the first-order shear deformation theory,the governing equation of free vibration of a functionally graded beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam axial displacement,transverse displacement,and cross-sectional rotation angle by considering the effects of shear deformation and rotational inertia of the beam cross-section.Then,ignoring the shear deformation of the beam section and only considering the effect of the rotational inertia of the section,the governing equation of the beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam transverse displacement.Based on the differential quadrature method theory,the eigenvalue problem of ordinary differential equations is transformed into the eigenvalue problem of standard generalized algebraic equations.Finally,the first several natural frequencies of the beam can be calculated.The feasibility and accuracy of the improved DQM are verified using the finite element method(FEM)and combined with the results of relevant literature.
基金Supported by National 863 Program of China(2006AA10A113)Natural Science foundation of Zhejiang Province(Y306097)~~
文摘[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...
基金supported by the National Basic Research Program of China(Grant No.2012CB825602)National Natural Science Foundation of China(Grant Nos.41231067&41204110) in part by the Specialized Research Fund for State Key Laboratories of China
文摘Derivation of equivalent current systems(ECS)from a global magnetospheric magnetohydrodynamics(MHD)model is very useful in studying magnetosphere-ionosphere coupling,ground induction effects,and space weather forecast.In this study we introduce an improved method to derive the ECS from a global MHD model,which takes account of the obliqueness of the magnetic field lines.By comparing the ECS derived from this improved method and the previous method,we find that the main characteristics of the ECS derived from the two methods are generally consistent with each other,but the eastward-westward component of the geomagnetic perturbation calculated from the ECS derived from the improved method is much stronger than that from the previous method.We then compare the geomagnetic perturbation as a function of the interplanetary magnetic field(IMF)clock angle calculated from the ECS derived from both methods with the observations.The comparison indicates that the improved method can improve the performance of the simulation.Furthermore,it is found that the incomplete counterbalance of the geomagnetic effect produced by the ionospheric poloidal current and field-aligned current(FAC)contributes to most of the eastward-westward component of geomagnetic perturbation.
基金This work was supported by the National Natural Science Foundation of China under Grant No.60578051.
文摘An improved method of angle measurement is proposed based on a parallel plate interferometer. A position detection system is incorporated into a parallel plate interferometer in order to realize large deflection angle measurement. A reflecting mirror is introduced for increasing the measurement resolution. In experiments, a deflection angle of a measured target was measured within ～3° with high accuracy. And as a phase modulating interferometer, it was used to measure a small angular displacement with a repeatability of 5.5 × 10^-8 rad.
基金supported by the National Natural Science Foundation of China(Nos.11472228 and 11202174)the Sichuan Provincial Youth Science and Technology Innovation Team(No.2013TD0004)
文摘This study successfully deals with the inhomogeneous dimension problem of load separation assumption, which is the theoretical basis of the normalization method. According to the dimensionless load separation principle, the normalization method has been improved by intro- ducing a forcible blunting correction. With the improved normalization method, the J-resistance curves of five different metallic materials of CT and SEB specimens are estimated. The forcible blunting correction of initial crack size plays an important role in the J-resistance curve estima- tion, which is closely related to the strain hardening level of material. The higher level of strain hardening leads to a greater difference in JQ determined by different slopes of the blunting line. If the blunting line coefficient recommended by ASTM E1820-11 is used in the improved nor- realization method, it will lead to greater fracture resistance than that processed by the blunting line coefficient recommended by ISO 12135-2002. Therefore, the influence of the blunting line on the determination of JQ must be taken into full account in the fracture toughness assessment of metallic materials.
基金Project supported by the National Natural Science Foundation of China (Grant No. 11171208)the Shanghai Leading Academic Discipline Project, China (Grant No. S30106)
文摘In this paper, an improved interpolating moving least-square （IIMLS） method is presented. The shape function of the IIMLS method satisfies the property of the Kronecker 5 function. The weight function used in the IIMLS method is nonsingular. Then the IIMLS method can overcome the difficulties caused by the singularity of the weight function in the IMLS method. The number of unknown coefficients in the trial function of the IIMLS method is less than that of the moving least-square （MLS） approximation. Then by combining the IIMLS method with the Galerkin weak form of the potential problem, the improved interpolating element-free Galerkin （IIEFG） method for two-dimensional potential problems is presented. Compared with the conventional element-free Galerkin （EFG） method, the IIEFG method can directly use the essential boundary conditions. Then the IIEFG method has higher accuracy. For demonstration, three numerical examples are solved using the IIEFG method.
基金supported by the National Natural Science Foundation of China (No. 41071322, 71031001)
文摘For natural water, method of water quality evaluation based on improved fuzzy matter-element evaluation method is presented. Two important parts are improved, the weights determining and fuzzy membership functions. The coefficient of variation of each indicator is used to determine the weight instead of traditional calculating superscales method. On the other hand, fuzzy matter-elements are constructed, and normal membership degrees are used instead of traditional trapezoidal ones. The composite fuzzy matter-elements with associated coefficient are constructed through associated transformation. The levels of natural water quality are determined according to the principle of maximum correlation. The improved fuzzy matter-element evaluation method is applied to evaluate water quality of the Luokou mainstream estuary at the first ten weeks in 2011 with the coefficient of variatiola method determining the weights. Water quality of Luokou mainstream estuary is dropping from level I to level II. The results of the improved evaluation method are basically the same as the official water quality. The variation coefficient method can reduce the workload, and overcome the adverse effects from abnormal values, compared with the traditional calculating superscales method. The results of improved fuzzy matter- element evaluation method are more credible than the ones of the traditional evaluation method. The improved evaluation method can use information of monitoring data more scientifically and comprehensively, and broaden a new evaluation method for water quality assessment.
基金National Natural Science Foundation of China, No.40771186.
文摘The relative importance of each pollution factor in analytical hierarchy process (AHP) method comes from pooling expert opinions in general. Because expert opinions are based on information and judgment criteria, determining their weight may lead to uncertainty.. Therefore, an improved AHP method had been developed. The process of the improved AHP method involved four key procedures. The weights of pollution factors were completely related to the objective monitoring data through the standardization of these procedures. The environmental comprehensive quality of water and sediment of Xuanwu Lake, Nanjing, China had been evaluated. The environmental quality comprehensive indices (EQCI) of the water in 1991-1995 and 1996-2000 were 3.32 and 1.85, respectively, indicating that the water quality improved. The EQCI of the sediment in 1995 and 2000 indicates that the sediment contamination decreased from 1995 to 2000. Such results agreed with the fact that the lake had been under comprehensive control. However, with the classical AHP method, the EQCI of the sediment in the northwestern part of the lake may have indicated that sediment contamination increased from 1995 to 2000. The discrepancy may have resulted from the judgment difference of the experts. The improved AHP method can avoid arbitrariness of subjective judgment and can reflect the real influential factors of environment pollution in different periods or regions.
