·AIM:To establish a new method to record visual evoked potential of rats and this method was applied to retinal cone degeneration (RCD) and congenital stationary night blindness (CSNB) rats to see whether it is f...·AIM:To establish a new method to record visual evoked potential of rats and this method was applied to retinal cone degeneration (RCD) and congenital stationary night blindness (CSNB) rats to see whether it is feasible.·METHODS:After anesthesia,the rats scalp surface was exposed and two stainless screws were implanted at 4mm in front of and 8mm behind Bregma.The front one was reference electrode and the latter one was recording electrode.The ground electrode was joined to the tail.And we recorded VEP of scotopic adaptation and photopic adaptation (The stimulus intensity of light :0.011,0.035,0.11,0.35,1.1 and 3.5cd·s/m2.They were all overlapped for 100 times.There were 2-5minutes intervals between different intensities.The frequency of stimulation was 1Hz and time course was 300 milliseconds).A week later,we recorded the VEP again in the same conditions.·RESULTS:The rats who were implanted with electrodes could live longer.When they were recorded,there was no disturbance and the waveforms were very clear and steady.There was no difference between the waveforms recorded by conventional method and those by implanted electrodes while the amplitude of new method was larger than conventional.We could get the same conclusion in RCD and CSNB rats.·CONCLUSION:Since the electrodes can be kept on the scalp for a longer time,we can not only observe dynamic changes of VEP but also observe the long-duration patho-logical changes in the rats.Because the positions of recording and reference electrodes were fixed,the system error can be reduced and the repeatability of experiments increased.·展开更多
With a Ni/GC ion implantation modified electrode as working electrode, in 0.1 mol/L HOAc-NaOAc (pH=4.62) solution, a sensitive reductive wave of ADM was obtained by linear sweep voltammetry. The peak potential was -0....With a Ni/GC ion implantation modified electrode as working electrode, in 0.1 mol/L HOAc-NaOAc (pH=4.62) solution, a sensitive reductive wave of ADM was obtained by linear sweep voltammetry. The peak potential was -0.55 V (vs.SCE). The peak current is proportional to the concentration of ADM with a detection limit of 6.9 X 10(-8) mol/L. The behavior of the reduction wave was studied. The experiments of AES and XPS showed that Ni was surely implanted into the surface of the GCE and the implanted Ni at the GCE improved the electrocatalytic activity.展开更多
In a 0. 10 mol/L HAc-NaAc buffer solution (pH = 4. 59), a sensitive reduction peak of bleomycin was observed by linear sweep voltammetry at a Co/GC ion implantation modified electrode. The peak potential was-0. 73 V(i...In a 0. 10 mol/L HAc-NaAc buffer solution (pH = 4. 59), a sensitive reduction peak of bleomycin was observed by linear sweep voltammetry at a Co/GC ion implantation modified electrode. The peak potential was-0. 73 V(iw. SCE). The peak current was proportional to the concentration of bleomycin over the range of 5.0 × 10-8-1.0× 10-6 and 1.0× 10-6-1. 0 × 10-5 mol/L with a detection limit of 2.0 × 10-8 mol/L. The electrochemical behavior of the reduction peak of bleomycin at the Co/GC modified electrode was studied by linear sweep and cyclic voltammetry and applied to the determination of bleomycin in urine. This method is simple, rapid and reliable. The reduction process is quasi-reversible. The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE and the depth distribution of Co was in good agreement with Gooses normal distribution; the implanted Co at GCE improved the electrocatalytic activity.展开更多
Targeted muscle reinnervation has been proposed for reconstruction of neuromuscular function in amputees.However,it is unknown whether performing delayed targeted muscle reinnervation after nerve injury will affect re...Targeted muscle reinnervation has been proposed for reconstruction of neuromuscular function in amputees.However,it is unknown whether performing delayed targeted muscle reinnervation after nerve injury will affect restoration of function.In this rat nerve injury study,the median and musculocutaneous nerves of the forelimb were transected.The proximal median nerve stump was sutured to the distal musculocutaneous nerve stump immediately and 2 and 4 weeks after surgery to reinnervate the biceps brachii.After targeted muscle reinnervation,intramuscular myoelectric signals from the biceps brachii were recorded.Signal amplitude gradually increased with time.Biceps brachii myoelectric signals and muscle fiber morphology and grooming behavior did not significantly differ among rats subjected to delayed target muscle innervation for different periods.Targeted muscle reinnervation delayed for 4 weeks can acquire the same nerve function restoration effect as that of immediate reinnervation.展开更多
To collect neural activity data from awake, behaving freely animals, we develop miniaturized implantable recording system by the modem chip：Programmable System on Chip （PSoC） and through chronic electrodes in the c...To collect neural activity data from awake, behaving freely animals, we develop miniaturized implantable recording system by the modem chip：Programmable System on Chip （PSoC） and through chronic electrodes in the cortex. With PSoC family member CY8C29466,the system completed operational and instrument amplifiers, filters, timers, AD convertors, and serial communication, etc. The signal processing was dealt with virtual instrument technology. All of these factors can significantly affect the price and development cycle of the project. The result showed that the system was able to record and analyze neural extrocellular discharge generated by neurons continuously for a week or more. This is very useful for the interdisciplinary research of neuroscience and information engineering technique. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.展开更多
To collect neuronal activity data from awake, freely behaving animals, we developed miniature telemetry recording system. The integrated system consists of four major components： l） Microelectrodes and micro-driver ...To collect neuronal activity data from awake, freely behaving animals, we developed miniature telemetry recording system. The integrated system consists of four major components： l） Microelectrodes and micro-driver assembly, 2） analog front end （AFE）, 3） programmable system on chip （PSoC）, and 4） ra- dio transceiver and the LabVIEW were used as a platform for the graphic user interface. The result showed the system was able to record and analyze neuronal recordings in freely moving animals and lasted continuously for a time period of a week or more. This is very useful for the study of the interdisciplinary research of neu- roscience and information engineering techniques. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.展开更多
Implantable neural electrodes are key components of brain-computer interfaces(BCI),but the mismatch in mechanical and biological properties between electrode materials and brain tissue can lead to foreign body reactio...Implantable neural electrodes are key components of brain-computer interfaces(BCI),but the mismatch in mechanical and biological properties between electrode materials and brain tissue can lead to foreign body reactions and glial scarring,and subsequently compromise the long-term stability of electrical signal transmission.In this study,we proposed a new concept for the design and bioaugmentation of implantable electrodes(bio-array electrodes)featuring a heterogeneous gradient structure.Different composite polyaniline-gelatin-alginate based conductive hydrogel formulations were developed for electrode surface coating.In addition,the design,materials,and performance of the developed electrode was optimized through a combination of numerical simulations and physio-chemical characterizations.The long-term biological performance of the bio-array electrodes were investigated in vivo using a C57 mouse model.It was found that compared to metal array electrodes,the surface charge of the bio-array electrodes increased by 1.74 times,and the impedance at 1 kHz decreased by 63.17%,with a doubling of the average capacitance.Long-term animal experiments showed that the bio-array electrodes could consistently record 2.5 times more signals than those of the metal array electrodes,and the signal-to-noise ratio based on action potentials was 2.1 times higher.The study investigated the mechanisms of suppressing the scarring effect by the bioaugmented design,revealing reduces brain damage as a result of the interface biocompatibility between the bio-array electrodes and brain tissue,and confirmed the long-term in vivo stability of the bio-array electrodes.展开更多
Electrochemical investigation of Bleomycin (BLM) and its interaction with DNAat a Co/GC ion implantation modified electrode was reported for the first time. The electrochemicalbehavior of BLM at Co/GC modified electro...Electrochemical investigation of Bleomycin (BLM) and its interaction with DNAat a Co/GC ion implantation modified electrode was reported for the first time. The electrochemicalbehavior of BLM at Co/GC modified electrode has been studied by linear sweep and cyclicvoltammetry, The reaction of BLM with DNA formed an electrochemically nonactive complex, whichresulted in a decrease in the BLM equilibrium concentration and its reduction current. The decreasein peak current was proportional to DNA concentration and could be used to determination DNAconcentration. The equilibrium constant of DNA-BLM complex β was determined to be 5.16 x 10^(16).The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE( glassycarbon electrode) and the depth distribution of Co was in good agreement with Gaussian normaldistribution; the implanted Co at GCE improved the electrocatalytic activity.展开更多
基金National Natural Science Foundation of China (No.30371517,30571999)
文摘·AIM:To establish a new method to record visual evoked potential of rats and this method was applied to retinal cone degeneration (RCD) and congenital stationary night blindness (CSNB) rats to see whether it is feasible.·METHODS:After anesthesia,the rats scalp surface was exposed and two stainless screws were implanted at 4mm in front of and 8mm behind Bregma.The front one was reference electrode and the latter one was recording electrode.The ground electrode was joined to the tail.And we recorded VEP of scotopic adaptation and photopic adaptation (The stimulus intensity of light :0.011,0.035,0.11,0.35,1.1 and 3.5cd·s/m2.They were all overlapped for 100 times.There were 2-5minutes intervals between different intensities.The frequency of stimulation was 1Hz and time course was 300 milliseconds).A week later,we recorded the VEP again in the same conditions.·RESULTS:The rats who were implanted with electrodes could live longer.When they were recorded,there was no disturbance and the waveforms were very clear and steady.There was no difference between the waveforms recorded by conventional method and those by implanted electrodes while the amplitude of new method was larger than conventional.We could get the same conclusion in RCD and CSNB rats.·CONCLUSION:Since the electrodes can be kept on the scalp for a longer time,we can not only observe dynamic changes of VEP but also observe the long-duration patho-logical changes in the rats.Because the positions of recording and reference electrodes were fixed,the system error can be reduced and the repeatability of experiments increased.·
文摘With a Ni/GC ion implantation modified electrode as working electrode, in 0.1 mol/L HOAc-NaOAc (pH=4.62) solution, a sensitive reductive wave of ADM was obtained by linear sweep voltammetry. The peak potential was -0.55 V (vs.SCE). The peak current is proportional to the concentration of ADM with a detection limit of 6.9 X 10(-8) mol/L. The behavior of the reduction wave was studied. The experiments of AES and XPS showed that Ni was surely implanted into the surface of the GCE and the implanted Ni at the GCE improved the electrocatalytic activity.
基金National Science Foundation of China(No.98002709)and Doctoral Program Foundation ofInstitution of Higher Education.
文摘In a 0. 10 mol/L HAc-NaAc buffer solution (pH = 4. 59), a sensitive reduction peak of bleomycin was observed by linear sweep voltammetry at a Co/GC ion implantation modified electrode. The peak potential was-0. 73 V(iw. SCE). The peak current was proportional to the concentration of bleomycin over the range of 5.0 × 10-8-1.0× 10-6 and 1.0× 10-6-1. 0 × 10-5 mol/L with a detection limit of 2.0 × 10-8 mol/L. The electrochemical behavior of the reduction peak of bleomycin at the Co/GC modified electrode was studied by linear sweep and cyclic voltammetry and applied to the determination of bleomycin in urine. This method is simple, rapid and reliable. The reduction process is quasi-reversible. The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE and the depth distribution of Co was in good agreement with Gooses normal distribution; the implanted Co at GCE improved the electrocatalytic activity.
基金supported in part by the National Natural Science Foundation of China,Nos.U1913601,81927804the Key-Area Research and Development Program of Guangdong Province,No.2020B0909020004(GL)the National Natural Science Foundation of China,Nos.81960419,82260456(both to LY)。
文摘Targeted muscle reinnervation has been proposed for reconstruction of neuromuscular function in amputees.However,it is unknown whether performing delayed targeted muscle reinnervation after nerve injury will affect restoration of function.In this rat nerve injury study,the median and musculocutaneous nerves of the forelimb were transected.The proximal median nerve stump was sutured to the distal musculocutaneous nerve stump immediately and 2 and 4 weeks after surgery to reinnervate the biceps brachii.After targeted muscle reinnervation,intramuscular myoelectric signals from the biceps brachii were recorded.Signal amplitude gradually increased with time.Biceps brachii myoelectric signals and muscle fiber morphology and grooming behavior did not significantly differ among rats subjected to delayed target muscle innervation for different periods.Targeted muscle reinnervation delayed for 4 weeks can acquire the same nerve function restoration effect as that of immediate reinnervation.
基金Shandong Province Nature Science FoundationGrant number:Y2007C02+1 种基金Science Development PlanGrant number:2006GG3204006
文摘To collect neural activity data from awake, behaving freely animals, we develop miniaturized implantable recording system by the modem chip：Programmable System on Chip （PSoC） and through chronic electrodes in the cortex. With PSoC family member CY8C29466,the system completed operational and instrument amplifiers, filters, timers, AD convertors, and serial communication, etc. The signal processing was dealt with virtual instrument technology. All of these factors can significantly affect the price and development cycle of the project. The result showed that the system was able to record and analyze neural extrocellular discharge generated by neurons continuously for a week or more. This is very useful for the interdisciplinary research of neuroscience and information engineering technique. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.
基金supported by the Shandong Province Nature Science Foundation(Grant No.ZR2010CM055)Science Development Plan Project(Grant No.2010GGX10133)
文摘To collect neuronal activity data from awake, freely behaving animals, we developed miniature telemetry recording system. The integrated system consists of four major components： l） Microelectrodes and micro-driver assembly, 2） analog front end （AFE）, 3） programmable system on chip （PSoC）, and 4） ra- dio transceiver and the LabVIEW were used as a platform for the graphic user interface. The result showed the system was able to record and analyze neuronal recordings in freely moving animals and lasted continuously for a time period of a week or more. This is very useful for the study of the interdisciplinary research of neu- roscience and information engineering techniques. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.
基金supported by the Program of the National Natural Science Foundation of China[52275291],[52435006]the Program for Innovation Team of Shaanxi Province(2023-CX-TD-17)the Fundamental Research Funds for the Central Universities.
文摘Implantable neural electrodes are key components of brain-computer interfaces(BCI),but the mismatch in mechanical and biological properties between electrode materials and brain tissue can lead to foreign body reactions and glial scarring,and subsequently compromise the long-term stability of electrical signal transmission.In this study,we proposed a new concept for the design and bioaugmentation of implantable electrodes(bio-array electrodes)featuring a heterogeneous gradient structure.Different composite polyaniline-gelatin-alginate based conductive hydrogel formulations were developed for electrode surface coating.In addition,the design,materials,and performance of the developed electrode was optimized through a combination of numerical simulations and physio-chemical characterizations.The long-term biological performance of the bio-array electrodes were investigated in vivo using a C57 mouse model.It was found that compared to metal array electrodes,the surface charge of the bio-array electrodes increased by 1.74 times,and the impedance at 1 kHz decreased by 63.17%,with a doubling of the average capacitance.Long-term animal experiments showed that the bio-array electrodes could consistently record 2.5 times more signals than those of the metal array electrodes,and the signal-to-noise ratio based on action potentials was 2.1 times higher.The study investigated the mechanisms of suppressing the scarring effect by the bioaugmented design,revealing reduces brain damage as a result of the interface biocompatibility between the bio-array electrodes and brain tissue,and confirmed the long-term in vivo stability of the bio-array electrodes.
文摘Electrochemical investigation of Bleomycin (BLM) and its interaction with DNAat a Co/GC ion implantation modified electrode was reported for the first time. The electrochemicalbehavior of BLM at Co/GC modified electrode has been studied by linear sweep and cyclicvoltammetry, The reaction of BLM with DNA formed an electrochemically nonactive complex, whichresulted in a decrease in the BLM equilibrium concentration and its reduction current. The decreasein peak current was proportional to DNA concentration and could be used to determination DNAconcentration. The equilibrium constant of DNA-BLM complex β was determined to be 5.16 x 10^(16).The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE( glassycarbon electrode) and the depth distribution of Co was in good agreement with Gaussian normaldistribution; the implanted Co at GCE improved the electrocatalytic activity.
