To address postoperative melanoma issues of high recurrence and poor wound healing,we developed a nanocomposite hydrogel wound patch(M_(xNd/yCe)@M SAC)for cascade sequential therapy.Methotrexate(MTX)loaded Nd/Ce-doped...To address postoperative melanoma issues of high recurrence and poor wound healing,we developed a nanocomposite hydrogel wound patch(M_(xNd/yCe)@M SAC)for cascade sequential therapy.Methotrexate(MTX)loaded Nd/Ce-doped mesoporous bioactive glass was encapsulated in a sulfobetaine-polyacrylamide-carboxymethyl chitosan hydrogel via thermal polymerization.Under the acidic microenvironment of the residual tumor,the amide bonds in M_(xNd/yCe)@M SAC are broken,releasing M_(xNd/yCe)@M.M_(xNd/yCe)@M targets tumor cells,and the combined therapy of mild photothermal treatment and chemotherapy ablates tumor cells.Subsequently,the continuously released MTX downregulates the expression of pro-inflammatory factors and reshapes the immune microenvironment.Finally,M_(xNd/yCe)@M disintegrates,releasing Si^(4+),Ca^(2+),P^(5+)and Ce^(3+)ions,which can effectively promote angiogenesis and tissue repair.In the postoperative melanoma model,this method effectively cleared the residual tumors after surgery,shortened the inflammatory period,and promoted tissue regeneration.In conclusion,the nanocomposite hydrogel wound patch for cascade sequential treatment prepared in this study has excellent anti-tumor,immune regulation and tissue repair properties,providing prospective insights for the postoperative treatment of malignant melanoma.展开更多
基金supported by the National Key Research and Development Program of China(No.2022YFC2106100)the National Natural Science Foundation of China(No.22078265).
文摘To address postoperative melanoma issues of high recurrence and poor wound healing,we developed a nanocomposite hydrogel wound patch(M_(xNd/yCe)@M SAC)for cascade sequential therapy.Methotrexate(MTX)loaded Nd/Ce-doped mesoporous bioactive glass was encapsulated in a sulfobetaine-polyacrylamide-carboxymethyl chitosan hydrogel via thermal polymerization.Under the acidic microenvironment of the residual tumor,the amide bonds in M_(xNd/yCe)@M SAC are broken,releasing M_(xNd/yCe)@M.M_(xNd/yCe)@M targets tumor cells,and the combined therapy of mild photothermal treatment and chemotherapy ablates tumor cells.Subsequently,the continuously released MTX downregulates the expression of pro-inflammatory factors and reshapes the immune microenvironment.Finally,M_(xNd/yCe)@M disintegrates,releasing Si^(4+),Ca^(2+),P^(5+)and Ce^(3+)ions,which can effectively promote angiogenesis and tissue repair.In the postoperative melanoma model,this method effectively cleared the residual tumors after surgery,shortened the inflammatory period,and promoted tissue regeneration.In conclusion,the nanocomposite hydrogel wound patch for cascade sequential treatment prepared in this study has excellent anti-tumor,immune regulation and tissue repair properties,providing prospective insights for the postoperative treatment of malignant melanoma.
