The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we ...The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women.By 16S rRNA sequencing,we identified four core vaginal microbiota with a prevalence of over 90%and a total median abundance of 98.8%.Twenty-four variables,including physiology,lifestyle behaviors,gynecologic history,social and environmental information,were found associated with the microbiome composition,of which bacterial vaginosis(BV)showed the largest effect size.Age was among the strongest explanatory variables and the vaginal microbiome dynamically succeeded with increasing age,especially with a composition turning point at the age of 45.Our mediation analyses indicated that the effects of age on the microbiome could be mediated by variables such as parity number and lifestyles.We further classified the vaginal microbiomes of the population into 13“Vagitypes”.Women with Lactobacillus iners-and Lactobacillus jensenii-dominated Vagitypes had significantly higher live birth rate than those with Vagitype dominated by Fannyhessea vaginae(53.40%,59.09%vs 21.43%;OR[95%CI]:3.62[1.12-14.87],5.39[1.27-27.36];P=0.031,P=0.021).This study provides a comprehensive overview of the associations between identified variables and the vaginal microbiome,representing an important step toward understanding of environment-microbe-host interactions.展开更多
基金funded by the National Key Research and Development Program of China 2021YFC2700400 and 2021YFC2700701 to H.Z.and S.G.Z.the Basic Science Center Program of NSFC 31988101,Shandong Provincial Key Research and Development Program 2020ZLYS02+4 种基金the Innovative Research Team of Highlevel Local Universities in Shanghai SHSMU-ZLCX20210200,to Z.-J.C.the National Natural Science Foundation of China 82421004,82192874,31871509,82071606,and 82071122 to H.Z.,S.G.Z.and Q.F.the National High-level Young Scientist Project Foundation(2019)and the Excellent Young Scientist Foundation of Shandong Province ZR202102230369 to Q.F.the Taishan Scholars Program of Shandong Province ts20190988 to H.Z.the Fundamental Research Funds of Shandong University 2023QNTDO04 to S.G.Z.
文摘The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women.By 16S rRNA sequencing,we identified four core vaginal microbiota with a prevalence of over 90%and a total median abundance of 98.8%.Twenty-four variables,including physiology,lifestyle behaviors,gynecologic history,social and environmental information,were found associated with the microbiome composition,of which bacterial vaginosis(BV)showed the largest effect size.Age was among the strongest explanatory variables and the vaginal microbiome dynamically succeeded with increasing age,especially with a composition turning point at the age of 45.Our mediation analyses indicated that the effects of age on the microbiome could be mediated by variables such as parity number and lifestyles.We further classified the vaginal microbiomes of the population into 13“Vagitypes”.Women with Lactobacillus iners-and Lactobacillus jensenii-dominated Vagitypes had significantly higher live birth rate than those with Vagitype dominated by Fannyhessea vaginae(53.40%,59.09%vs 21.43%;OR[95%CI]:3.62[1.12-14.87],5.39[1.27-27.36];P=0.031,P=0.021).This study provides a comprehensive overview of the associations between identified variables and the vaginal microbiome,representing an important step toward understanding of environment-microbe-host interactions.
