Objective:To develop a quautitative PCR method for detecting hookworm infection and quantification.Methods:A real-time PCR method was designed hased on the intergenic regionⅡof ribosomal DNA of the hookworm Neeator a...Objective:To develop a quautitative PCR method for detecting hookworm infection and quantification.Methods:A real-time PCR method was designed hased on the intergenic regionⅡof ribosomal DNA of the hookworm Neeator americanus.The deteetion limit of this method was compared with the microscopy-hased Kato-Katz method.The real-time PCR method was used to conduct an epidemiological survey of hookworm infection in southern Fujian Province of China.Result:The real-time PCR method was specific for detecting Necator americanus infection,and was more sensitive than conventional PCR or microscopy-based method.A preliminary survey for hookworm infection in villages of Fujian Province confirmed the high prevalence of hnokworm infections in the resident populations.In addition,the infection rate in women was significantly higher than thai of in men.Conclusions:A real-time PCR method is designed,which has increased detection sensitivity for more accurate epidemiological studies of hookworm infections,especially when intensity of the infection needs to he considered.展开更多
Hookworm infection is a relatively common cause of anemia in endemic areas.The most common hookworm species are Ancylostoma duodenale and Necator americanus.In this report we present a case of overt gastrointestinal b...Hookworm infection is a relatively common cause of anemia in endemic areas.The most common hookworm species are Ancylostoma duodenale and Necator americanus.In this report we present a case of overt gastrointestinal bleeding Wause of hookworm infection.Capsule endoscopy revealed many hookworms in the lumen of proximal jejunum where active bleeding was seen. The patient was successfully treated with Albendazole.展开更多
A quantitative PCR（q PCR） assay was used to quantify Ancylostoma caninum ova in wastewater and sludge samples.We estimated the average gene copy numbers for a single ovum using a mixed population of ova.The average ...A quantitative PCR（q PCR） assay was used to quantify Ancylostoma caninum ova in wastewater and sludge samples.We estimated the average gene copy numbers for a single ovum using a mixed population of ova.The average gene copy numbers derived from the mixed population were used to estimate numbers of hookworm ova in A.caninum seeded and unseeded wastewater and sludge samples.The newly developed qP CR assay estimated an average of3.7 × 10~3 gene copies per ovum,which was then validated by seeding known numbers of hookworm ova into treated wastewater.The qP CR estimated an average of（1.1 ± 0.1）,（8.6 ± 2.9）and（67.3 ± 10.4） ova for treated wastewater that was seeded with（1 ± 0）,（10 ± 2） and（100 ± 21）ova,respectively.The further application of the q PCR assay for the quantification of A.caninum ova was determined by seeding a known numbers of ova into the wastewater matrices.The qP CR results indicated that 50%,90% and 67% of treated wastewater（1 L）,raw wastewater（1 L）and sludge（~4 g） samples had variable numbers of A.caninum gene copies.After conversion of the q PCR estimated gene copy numbers to ova for treated wastewater,raw wastewater,and sludge samples,had an average of 0.02,1.24 and 67 ova,respectively.The result of this study indicated that qP CR can be used for the quantification of hookworm ova from wastewater and sludge samples;however,caution is advised in interpreting qP CR generated data for health risk assessment.展开更多
Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the...Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the presence of STH(e.g.,hookworms,roundworms and whipworms)in soil samples collected at twenty points within the perimeter of Campus do Vale(a university campus belonging to the Federal University of Rio Grande do Sul-UFRGS),during 2022 winter season.Considering the One Health perspective,human,animal and environmentrelated data from each sampling point were collected.All soil samples showed nematode larvae,representing natural components of soil biodiversity.Considering STH eggs,35%(n=7)of soil samples showed hookworm eggs(e.g.,from Necator americanus or Ancylostoma duodenale),10%(n=2)showed roundworm(Ascaris lumbricoides)eggs,and 5%(n=1)showed whipworm(Trichuris trichiura-like)eggs.Of note,10%of the sampling points showed the presence of rhabditiform hookworm larvae,5%showed Strongyloides stercoralis rhabditiform larvae and 5%had the presence of filariform hookworm larvae,indicating a risk of human percutaneous infection.The significant people circulation in Campus do Vale,in association with other environment-related factors,help to explain the prevalence of STH observed in this study.展开更多
Cutaneous larva migrans(CLM) represents the most common tropically acquired dermatosis.CLM is caused by infection with hookworm larvae in tropical and sub-tropical areas,and people who have a history of foreign travel...Cutaneous larva migrans(CLM) represents the most common tropically acquired dermatosis.CLM is caused by infection with hookworm larvae in tropical and sub-tropical areas,and people who have a history of foreign travel and of walking barefoot on sandy soil or beaches are at a high risk of getting infected with it.The diagnosis is usually made on the basis of the typical appearance of the lesion,intense itching and history of foreign travel.CLM is a common parasitic skin disease that can be easily prevented by wearing 'protective' footwear.A case of CLM is described in this article.展开更多
Objective Our objective was to systematically evaluate chemotherapy-based control of ascariasis and hookworm infection and make predictions of the effectiveness of repeated mass treatment at different levels of covera...Objective Our objective was to systematically evaluate chemotherapy-based control of ascariasis and hookworm infection and make predictions of the effectiveness of repeated mass treatment at different levels of coverage in highly endemic areas of China. Methods Field surveys were carried out to acquire the ascariasis and hookworm prevalence and intensity （mean worm burden） at baseline, one month and one year later. We calculated model parameters based on the survey data, then incorporated them into a quantitative framework to predict the prevalence and intensity one year later. Sensitivity analyses were performed to assess the influence of the chemotherapy measures on prevalence and intensity, and model simulations were performed to evaluate the feasibility of achieving the proposed transmission control criteria under different chemotherapy measures. Results The predicted prevalence and intensity one year from baseline were within the 95% confidence interval of actual values. As treatment frequency or coverage increased, the prevalence and intensity decreased. Model simulations show that many rounds of treatment are needed to maintain the prevalence at a low level in highly endemic areas of China. Conclusion We should select different combinations of treatment frequency, coverage and drug efficacy according to available resources and practical attainable conditions. Mathematical modeling could be used to help optimize the chemotherapeutic scheme aiming at specific parasitic species and areas, and to direct the establishment of soil-transmitted helminthiasis control criteria in China.展开更多
Hookworm is an illness caused by an internal sponger called a roundworm.Inferable from deprived cleanliness in the developing nations,hookworm infection is a primary source of concern for both motherly and baby grimne...Hookworm is an illness caused by an internal sponger called a roundworm.Inferable from deprived cleanliness in the developing nations,hookworm infection is a primary source of concern for both motherly and baby grimness.The current framework for hookworm detection is composed of hybrid convolutional neural networks;explicitly an edge extraction framework alongside a hookworm classification framework is developed.To consolidate the cylindrical zones obtained from the edge extraction framework and the trait map acquired into the hookworm scientific categorization framework,pooling layers are proposed.The hookworms display different profiles,widths,and bend directions.These challenges make it difficult for customized hookworm detection.In the proposed method,a contourlet change was used with the development of the Hookworm detection.In this study,standard deviation,skewness,entropy,mean,and vitality were used for separating the highlights of the each form.These estimations were found to be accurate.AdaBoost classifier was utilized to characterize the hookworm pictures.In this paper,the exactness and the territory under bend examination in identifying the hookworm demonstrate its scientific relevance.展开更多
Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA...Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA container. Of the total of three hundred and thirty four (334) subjects, one hundred and fifty two (152) were Females and one hundred and eighty two (182) were Males. Intestinal parasite assessment was done by Direct Smear technique and Formol-Ether concentration methods. Hemoglobin concentration was analyzed using Cyanmethaemoglobin method. Thirty (30) subjects were infested with Ascaris lumbricoides (single infestation), Ninety Five (95) subjects were infested with Ascaris lumbricoides and Hookworm (Double infestation) and Forty Two (42) subjects were infested with Ascaris lumbricoides, Hookworm, Entamoeba histolytica and Trichuris trichiura (Multiple infestation). The Mean ± Standard Deviation of Hemoglobin concentration of the various infestation types against the control subject shows a statistically significant decrease展开更多
The aim of this study is to determine the Socio Economic Status and Red Blood Cell Hemoglobin concentration alteration in type 2 Diabetes mellitus patients attending Diabetic Clinic in Benin City, Nigeria. The sample ...The aim of this study is to determine the Socio Economic Status and Red Blood Cell Hemoglobin concentration alteration in type 2 Diabetes mellitus patients attending Diabetic Clinic in Benin City, Nigeria. The sample population consists of 142 subjects, 71 of patients were known in Type 2 Diabetes mellitus already on drugs and were confirmed to be Diabetic using Glucose oxidase method while the other 71 subjects were age matched apparently healthy control subject on routine check up, they were confirmed to be non Diabetic using the Glucose oxidase method. Hemoglobin concentrations were done using the Cyanmethemoglobin method. Those under the low income Socio-Economic status had the highest incidence of type 2 Diabetes mellitus (49%) of the Diabetic population, closely followed by the middle income Socio-Economic status (35%) of the Diabetic populations. The Mean ± S.D of Hemoglobin concentration of control subjects against Mean ± S.D of Hemoglobin concentration of the various Socio-Economic status of Males and Females type 2 Diabetes mellitus patient show a statistically significant decrease展开更多
Cutaneous larva migrans (CLM) is a zoonotic hookworm infection of dogs and cats commonly found in low-income countries in the tropical and subtropical regions and travellers to these regions. It is caused by invasion ...Cutaneous larva migrans (CLM) is a zoonotic hookworm infection of dogs and cats commonly found in low-income countries in the tropical and subtropical regions and travellers to these regions. It is caused by invasion of the skin by the parasitic larvae of hookworms which, after entry, move under the skin causing an inflammatory reaction resulting in a single or multiple tracks. Patients commonly present with a progressive, itchy, erythematous serpiginous skin rash, affecting the feet, although it can affect any other parts of the body. The diagnosis is mainly clinical, based on history and physical examination. Treatment of this condition can effectively be achieved with either albendazole or ivermectin. We present a case of a 7-year-old boy who was diagnosed with CLM after presenting to the hospital with a history of a progressive itchy rash with tracks on the left foot. Although CLM is a self-limiting disease, it causes a lot of suffering and, therefore, it is of public concern. There is a need to increase awareness of this disease among health workers, and to implement and promote preventive measures against the disease since the cause is known.展开更多
Background Malaria and soil-transmitted helminths(STH)represent significant public health challenges in tropi-cal regions,particularly affecting children and impeding development.This study investigates the co-morbidi...Background Malaria and soil-transmitted helminths(STH)represent significant public health challenges in tropi-cal regions,particularly affecting children and impeding development.This study investigates the co-morbidity of malaria,caused by Plasmodium spp.,and STH infections,including Ascaris lumbricoides(roundworm),Ancylostoma duodenale and Necator americanus(hookworm),and Trichuris trichiura(whipworm),in Nigeria.Methods We utilized malaria prevalence data from the Nigeria Malaria Indicators Survey(NMIS)for the years 2010 and 2015 and STH prevalence data from the Expanded Special Project for Elimination of Neglected Tropical Diseases(ESPEN)portal,covering the years 1978-2014.A Bayesian coregionalization model was employed to analyze the prev-alence and incidence of malaria and STH,linking these data to climatic factors such as temperature and precipita-tion.The study's findings highlight significant co-morbidity between malaria and STH,particularly in the southsouth andsoutheastregions.Results Our analysis reveals notable regional disparities:malaria prevalence is highest in the northwest and north-central regions,while Ascaris lumbricoides is widespread in both northern and southern states.Ancylostoma duo-denale and Necator americanus(Hookworm)are predominantly found in the southwest,and Trichuris trichiura,though less prevalent,is significant in specific areas.Substantial co-morbidity between malaria and STH was observed,particularly in the South-South and southeast regions,indicating a compounded health burden.Furthermore,climatic factors significantly influence disease distribution;higher temperatures correlate with increased malaria prevalence,although temperature has a minimal effect on STH prevalence and incidence.In contrast,precipitation is positively associated with both malaria and STH incidence.Conclusions These findings enhance our understanding of the spatial distribution and risk factors associated with malaria and STH in Nigeria,providing vital insights for the development of public health policies and targeted intervention strategies.展开更多
Background Hookworm disease remains a global health issue.In China,it persists with a 0.67%infection rate and uneven distribution in 2021.Optimized control strategies are needed.This study aims to optimize interventio...Background Hookworm disease remains a global health issue.In China,it persists with a 0.67%infection rate and uneven distribution in 2021.Optimized control strategies are needed.This study aims to optimize intervention strategies for hookworm disease in China.Methods Structural analysis and parameter estimation were conducted using system dynamics theory.Key variables were identified via the Delphi method,leading to the creation of a causal loop diagram(CLD)and stock flow chart(SFC).Based on the SFC,parameter estimation and quantitative relationships were established and the model was validated.A cost-effectiveness model was then integrated into the intervention mechanism model.Various intervention measures were tested in the model to determine their cost-effectiveness ratio(CER)and effectiveness.Generalized linear models were constructed from simulation data,accounting for the impact of survey sites.The results were used to develop an optimized strategy for hookworm disease control.Results In comparing drug treatment methods,whole population deworming(WPD)and key population deworming(KPD)showed lower CERs than examination and voluntarily deworming(EVD),saving 384.79-504.64 CNY and 354.35-506.21 CNY per infection reduced,respectively(P<0.001).For WPD or KPD alone,CER decreased with increased drug coverage.For examination and deworming(ED)and EVD,CER was highest at 30%coverage for a 1-year intervention,but at 90%coverage for 2-5 years(P<0.05).WPD,ED,and EVD had higher infection reduction rates than KPD,with ratios of 0.14-0.25,0.10-0.19,and 0.08-0.17,respectively,over 1-5 years(P<0.001).Continuous health education over 1-5 years showed that increasing coverage from a 10%baseline led to enhancing cost-effectiveness and intervention outcomes.Conclusions In high-endemic areas(infection rate≥20%)in China,prioritize WPD for better cost-effectiveness and outcomes.In medium-endemic areas(5%≤infection rate<20%)where WPD isn't feasible,use ED for cost-effectiveness and KPD for infection reduction,based on local needs.In low-endemic areas(infection rate<5%),encourage voluntary examination and treatment due to limited cost-effectiveness of mass treatment.Combining drug treatment with extensive health education can enhance long-term control effect.This strategy can guide control efforts for hookwormdiseases in China.展开更多
In the field of landscape epidemiology,the contribution of machine learning(ML)to modeling of epidemiological risk scenarios presents itself as a good alternative.This study aims to break with the”black box”paradigm...In the field of landscape epidemiology,the contribution of machine learning(ML)to modeling of epidemiological risk scenarios presents itself as a good alternative.This study aims to break with the”black box”paradigm that underlies the application of automatic learning techniques by using SHAP to determine the contribution of each variable in ML models applied to geospatial health,using the prevalence of hookworms,intestinal parasites,in Ethiopia,where they are widely distributed;the country bears the third-highest burden of hookworm in Sub-Saharan Africa.XGBoost software was used,a very popular ML model,to fit and analyze the data.The Python SHAP library was used to understand the importance in the trained model,of the variables for predictions.The description of the contribution of these variables on a particular prediction was obtained,using different types of plot methods.The results show that the ML models are superior to the classical statistical models;not only demonstrating similar results but also explaining,by using the SHAP package,the influence and interactions between the variables in the generated models.This analysis provides information to help understand the epidemiological problem presented and provides a tool for similar studies.展开更多
Objective To determine the length of protection by murine immunization with living third stage hookworm larvae (L 3) as measured by reduction in worm burden and host serologic antibody responses Methods Outbred male (...Objective To determine the length of protection by murine immunization with living third stage hookworm larvae (L 3) as measured by reduction in worm burden and host serologic antibody responses Methods Outbred male (Kunming strain) mice were immunized subcutaneously with 500 L 3 once every 2 weeks for a total of immunization for 3 times, and then challenged orally with 1000 L 3 for 1 to 8 weeks after the final immunization Host protective immunity was determined both by the reduction in worm burden as measured by the number of L 3 recovered from murine lungs 48 hour post challenge, as well as by measurement of circulating antibodies Histopathological responses were also examined Non immunized mice served as negative controls Results The protection by L 3 immunization declined over time One or 2 weeks after the final immunization, worm burdens were reduced 72% and 77 5% after challenge respectively In contrast, only 37% reduction in worm burden was observed when the L 3 challenge was delayed by 4 weeks and protection was almost entirely lost when there was an 8 week delay between the time of final immunization and challenge The reduced level of protection over time partially correlated with diminishing L 3 specific antibody responses Host inflammation in the lungs of immunized mice also diminished Conclusion The protection afforded by living L 3 immunization is maximal for the first two weeks after immunization, but then declines significantly over the ensuing展开更多
Background:Humoral and cellular immune responses play protective roles against Mycobacterium tuberculosis(MTB)infection.However,hookworm infection decreases the immune response to hookworm and bystander antigens.Curre...Background:Humoral and cellular immune responses play protective roles against Mycobacterium tuberculosis(MTB)infection.However,hookworm infection decreases the immune response to hookworm and bystander antigens.Currently,immune responses to co-infection of MTB and hookworm are still unknown,although co-infection has been one of the public health problems in co-endemic areas of pulmonary tuberculosis(PTB)and hookworm disease.Therefore,it is essential to evaluate B and T cell immune responses to the co-infection.Methods:Seventeen PTB cases co-infected with hookworm,26 PTB cases,15 patients with hookworm infection,and 24 healthy controls without PTB or hookworm infection were enrolled in the study.Expressions of CD3,CD4,CD8,CD10,CD19,CD20,CD21,CD25,CD27,CD38,FoxP3,and PD-1 were assessed on B and T cell subsets using multicolor flow cytometry.Results:For the B cell(CD19+)subsets,naïve B cells(CD10−CD27−CD21+CD20+),plasma cells(CD10−CD27+CD21−CD20−),and tissue-like memory B cells(CD10−CD27−CD21−CD20+)had higher proportions,whilst resting memory B cells(CD10−CD27+CD21+CD20+)had lower proportions in the group co-infected with MTB and hookworm as compared to other groups.Frequencies of activated memory B cells(CD10−CD27+CD21−CD20+)did not differ among the four groups.For the T cell(CD3+)subsets,frequencies of regulatory T cells(CD4+CD25+Foxp3+)and exhausted CD4+and CD8+T cells(CD4+PD-1+and CD8+PD-1+)were higher,and frequencies of activated CD4+and CD8+T cells(CD4+CD38+and CD8+CD38+)were lower in the co-infected group as compared to the other groups.Conclusion:The change patterns of the cell profile of circulating lymphocytes were indentified in human co-infection of MTB and hookworm,which might indicate that the humoral and cellular immune responses are more suppressed.展开更多
Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Cani...Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Caninum) , were prepared as the source of antigen. Enzyme linked immunoelectrotransfer blotting (EITB) was enployed as an immunodiagnostic method. Results Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm infected patients (serum dilution 1∶200; antigen centrifuged at 36 000 r/m for 20 minutes, but not by sera from negative controls. Conclusion The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.展开更多
Dear Editor,Hookworm is an important tropical nematode.The worm is a roundworm that can be seen in many tropical areas.Basically,this roundworm can cause gastrointestinal infection and chronic infection and result in ...Dear Editor,Hookworm is an important tropical nematode.The worm is a roundworm that can be seen in many tropical areas.Basically,this roundworm can cause gastrointestinal infection and chronic infection and result in hypochromic microcytic anemia,which is the common public health problem.Focusing on the worm’s life cycle,the hookworm egg will be passed from intestine of infected host and hatched as larva in the soil.Based on this information,bearfooted walking is at risk.In general,hookworm is a geohelminth.展开更多
基金supported by the Fujian Provincial Grants(2008N2005,2009-CXB-67)Xiamen City Science and Technology Grant(3502Z20094021)
文摘Objective:To develop a quautitative PCR method for detecting hookworm infection and quantification.Methods:A real-time PCR method was designed hased on the intergenic regionⅡof ribosomal DNA of the hookworm Neeator americanus.The deteetion limit of this method was compared with the microscopy-hased Kato-Katz method.The real-time PCR method was used to conduct an epidemiological survey of hookworm infection in southern Fujian Province of China.Result:The real-time PCR method was specific for detecting Necator americanus infection,and was more sensitive than conventional PCR or microscopy-based method.A preliminary survey for hookworm infection in villages of Fujian Province confirmed the high prevalence of hnokworm infections in the resident populations.In addition,the infection rate in women was significantly higher than thai of in men.Conclusions:A real-time PCR method is designed,which has increased detection sensitivity for more accurate epidemiological studies of hookworm infections,especially when intensity of the infection needs to he considered.
文摘Hookworm infection is a relatively common cause of anemia in endemic areas.The most common hookworm species are Ancylostoma duodenale and Necator americanus.In this report we present a case of overt gastrointestinal bleeding Wause of hookworm infection.Capsule endoscopy revealed many hookworms in the lumen of proximal jejunum where active bleeding was seen. The patient was successfully treated with Albendazole.
基金financial support from the Water Corporation(No.XX-09-13910)WA and the Commonwealth Scientific and Industry Research Organisation(CSIRO)(Grant No:EOP 77576),Land and Water Flagship
文摘A quantitative PCR（q PCR） assay was used to quantify Ancylostoma caninum ova in wastewater and sludge samples.We estimated the average gene copy numbers for a single ovum using a mixed population of ova.The average gene copy numbers derived from the mixed population were used to estimate numbers of hookworm ova in A.caninum seeded and unseeded wastewater and sludge samples.The newly developed qP CR assay estimated an average of3.7 × 10~3 gene copies per ovum,which was then validated by seeding known numbers of hookworm ova into treated wastewater.The qP CR estimated an average of（1.1 ± 0.1）,（8.6 ± 2.9）and（67.3 ± 10.4） ova for treated wastewater that was seeded with（1 ± 0）,（10 ± 2） and（100 ± 21）ova,respectively.The further application of the q PCR assay for the quantification of A.caninum ova was determined by seeding a known numbers of ova into the wastewater matrices.The qP CR results indicated that 50%,90% and 67% of treated wastewater（1 L）,raw wastewater（1 L）and sludge（~4 g） samples had variable numbers of A.caninum gene copies.After conversion of the q PCR estimated gene copy numbers to ova for treated wastewater,raw wastewater,and sludge samples,had an average of 0.02,1.24 and 67 ova,respectively.The result of this study indicated that qP CR can be used for the quantification of hookworm ova from wastewater and sludge samples;however,caution is advised in interpreting qP CR generated data for health risk assessment.
基金Marina Ziliotto received a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES(Brazil).Joel Henrique Ellwanger receives a postdoctoral fellowship from CAPES(Programa Nacional de Pós-Doutorado-PNPD/CAPES,Brazil).JoséArtur Bogo Chies receives a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq(Bolsa de Produtividade em Pesquisa-Nível 1A,CNPq,Brazil)and has research project funded by CAPES(CAPES AUXPE 686/2020,Brazil).
文摘Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the presence of STH(e.g.,hookworms,roundworms and whipworms)in soil samples collected at twenty points within the perimeter of Campus do Vale(a university campus belonging to the Federal University of Rio Grande do Sul-UFRGS),during 2022 winter season.Considering the One Health perspective,human,animal and environmentrelated data from each sampling point were collected.All soil samples showed nematode larvae,representing natural components of soil biodiversity.Considering STH eggs,35%(n=7)of soil samples showed hookworm eggs(e.g.,from Necator americanus or Ancylostoma duodenale),10%(n=2)showed roundworm(Ascaris lumbricoides)eggs,and 5%(n=1)showed whipworm(Trichuris trichiura-like)eggs.Of note,10%of the sampling points showed the presence of rhabditiform hookworm larvae,5%showed Strongyloides stercoralis rhabditiform larvae and 5%had the presence of filariform hookworm larvae,indicating a risk of human percutaneous infection.The significant people circulation in Campus do Vale,in association with other environment-related factors,help to explain the prevalence of STH observed in this study.
文摘Cutaneous larva migrans(CLM) represents the most common tropically acquired dermatosis.CLM is caused by infection with hookworm larvae in tropical and sub-tropical areas,and people who have a history of foreign travel and of walking barefoot on sandy soil or beaches are at a high risk of getting infected with it.The diagnosis is usually made on the basis of the typical appearance of the lesion,intense itching and history of foreign travel.CLM is a common parasitic skin disease that can be easily prevented by wearing 'protective' footwear.A case of CLM is described in this article.
基金supported by the Special Fund for National Health Scientific Research in the Public Interest by the Ministry of Health of China (No.200802023)
文摘Objective Our objective was to systematically evaluate chemotherapy-based control of ascariasis and hookworm infection and make predictions of the effectiveness of repeated mass treatment at different levels of coverage in highly endemic areas of China. Methods Field surveys were carried out to acquire the ascariasis and hookworm prevalence and intensity （mean worm burden） at baseline, one month and one year later. We calculated model parameters based on the survey data, then incorporated them into a quantitative framework to predict the prevalence and intensity one year later. Sensitivity analyses were performed to assess the influence of the chemotherapy measures on prevalence and intensity, and model simulations were performed to evaluate the feasibility of achieving the proposed transmission control criteria under different chemotherapy measures. Results The predicted prevalence and intensity one year from baseline were within the 95% confidence interval of actual values. As treatment frequency or coverage increased, the prevalence and intensity decreased. Model simulations show that many rounds of treatment are needed to maintain the prevalence at a low level in highly endemic areas of China. Conclusion We should select different combinations of treatment frequency, coverage and drug efficacy according to available resources and practical attainable conditions. Mathematical modeling could be used to help optimize the chemotherapeutic scheme aiming at specific parasitic species and areas, and to direct the establishment of soil-transmitted helminthiasis control criteria in China.
基金supported by Korea Institute for Advancement of Technology(KIAT)grant funded by the Korea Government(MOTIE)(P0012724,The Competency Development Program for Industry Specialist)the Soonchunhyang University Research Fund.
文摘Hookworm is an illness caused by an internal sponger called a roundworm.Inferable from deprived cleanliness in the developing nations,hookworm infection is a primary source of concern for both motherly and baby grimness.The current framework for hookworm detection is composed of hybrid convolutional neural networks;explicitly an edge extraction framework alongside a hookworm classification framework is developed.To consolidate the cylindrical zones obtained from the edge extraction framework and the trait map acquired into the hookworm scientific categorization framework,pooling layers are proposed.The hookworms display different profiles,widths,and bend directions.These challenges make it difficult for customized hookworm detection.In the proposed method,a contourlet change was used with the development of the Hookworm detection.In this study,standard deviation,skewness,entropy,mean,and vitality were used for separating the highlights of the each form.These estimations were found to be accurate.AdaBoost classifier was utilized to characterize the hookworm pictures.In this paper,the exactness and the territory under bend examination in identifying the hookworm demonstrate its scientific relevance.
文摘Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA container. Of the total of three hundred and thirty four (334) subjects, one hundred and fifty two (152) were Females and one hundred and eighty two (182) were Males. Intestinal parasite assessment was done by Direct Smear technique and Formol-Ether concentration methods. Hemoglobin concentration was analyzed using Cyanmethaemoglobin method. Thirty (30) subjects were infested with Ascaris lumbricoides (single infestation), Ninety Five (95) subjects were infested with Ascaris lumbricoides and Hookworm (Double infestation) and Forty Two (42) subjects were infested with Ascaris lumbricoides, Hookworm, Entamoeba histolytica and Trichuris trichiura (Multiple infestation). The Mean ± Standard Deviation of Hemoglobin concentration of the various infestation types against the control subject shows a statistically significant decrease
文摘The aim of this study is to determine the Socio Economic Status and Red Blood Cell Hemoglobin concentration alteration in type 2 Diabetes mellitus patients attending Diabetic Clinic in Benin City, Nigeria. The sample population consists of 142 subjects, 71 of patients were known in Type 2 Diabetes mellitus already on drugs and were confirmed to be Diabetic using Glucose oxidase method while the other 71 subjects were age matched apparently healthy control subject on routine check up, they were confirmed to be non Diabetic using the Glucose oxidase method. Hemoglobin concentrations were done using the Cyanmethemoglobin method. Those under the low income Socio-Economic status had the highest incidence of type 2 Diabetes mellitus (49%) of the Diabetic population, closely followed by the middle income Socio-Economic status (35%) of the Diabetic populations. The Mean ± S.D of Hemoglobin concentration of control subjects against Mean ± S.D of Hemoglobin concentration of the various Socio-Economic status of Males and Females type 2 Diabetes mellitus patient show a statistically significant decrease
文摘Cutaneous larva migrans (CLM) is a zoonotic hookworm infection of dogs and cats commonly found in low-income countries in the tropical and subtropical regions and travellers to these regions. It is caused by invasion of the skin by the parasitic larvae of hookworms which, after entry, move under the skin causing an inflammatory reaction resulting in a single or multiple tracks. Patients commonly present with a progressive, itchy, erythematous serpiginous skin rash, affecting the feet, although it can affect any other parts of the body. The diagnosis is mainly clinical, based on history and physical examination. Treatment of this condition can effectively be achieved with either albendazole or ivermectin. We present a case of a 7-year-old boy who was diagnosed with CLM after presenting to the hospital with a history of a progressive itchy rash with tracks on the left foot. Although CLM is a self-limiting disease, it causes a lot of suffering and, therefore, it is of public concern. There is a need to increase awareness of this disease among health workers, and to implement and promote preventive measures against the disease since the cause is known.
文摘Background Malaria and soil-transmitted helminths(STH)represent significant public health challenges in tropi-cal regions,particularly affecting children and impeding development.This study investigates the co-morbidity of malaria,caused by Plasmodium spp.,and STH infections,including Ascaris lumbricoides(roundworm),Ancylostoma duodenale and Necator americanus(hookworm),and Trichuris trichiura(whipworm),in Nigeria.Methods We utilized malaria prevalence data from the Nigeria Malaria Indicators Survey(NMIS)for the years 2010 and 2015 and STH prevalence data from the Expanded Special Project for Elimination of Neglected Tropical Diseases(ESPEN)portal,covering the years 1978-2014.A Bayesian coregionalization model was employed to analyze the prev-alence and incidence of malaria and STH,linking these data to climatic factors such as temperature and precipita-tion.The study's findings highlight significant co-morbidity between malaria and STH,particularly in the southsouth andsoutheastregions.Results Our analysis reveals notable regional disparities:malaria prevalence is highest in the northwest and north-central regions,while Ascaris lumbricoides is widespread in both northern and southern states.Ancylostoma duo-denale and Necator americanus(Hookworm)are predominantly found in the southwest,and Trichuris trichiura,though less prevalent,is significant in specific areas.Substantial co-morbidity between malaria and STH was observed,particularly in the South-South and southeast regions,indicating a compounded health burden.Furthermore,climatic factors significantly influence disease distribution;higher temperatures correlate with increased malaria prevalence,although temperature has a minimal effect on STH prevalence and incidence.In contrast,precipitation is positively associated with both malaria and STH incidence.Conclusions These findings enhance our understanding of the spatial distribution and risk factors associated with malaria and STH in Nigeria,providing vital insights for the development of public health policies and targeted intervention strategies.
基金the National Natural Science Foundation of China(GrantNo.81973108).
文摘Background Hookworm disease remains a global health issue.In China,it persists with a 0.67%infection rate and uneven distribution in 2021.Optimized control strategies are needed.This study aims to optimize intervention strategies for hookworm disease in China.Methods Structural analysis and parameter estimation were conducted using system dynamics theory.Key variables were identified via the Delphi method,leading to the creation of a causal loop diagram(CLD)and stock flow chart(SFC).Based on the SFC,parameter estimation and quantitative relationships were established and the model was validated.A cost-effectiveness model was then integrated into the intervention mechanism model.Various intervention measures were tested in the model to determine their cost-effectiveness ratio(CER)and effectiveness.Generalized linear models were constructed from simulation data,accounting for the impact of survey sites.The results were used to develop an optimized strategy for hookworm disease control.Results In comparing drug treatment methods,whole population deworming(WPD)and key population deworming(KPD)showed lower CERs than examination and voluntarily deworming(EVD),saving 384.79-504.64 CNY and 354.35-506.21 CNY per infection reduced,respectively(P<0.001).For WPD or KPD alone,CER decreased with increased drug coverage.For examination and deworming(ED)and EVD,CER was highest at 30%coverage for a 1-year intervention,but at 90%coverage for 2-5 years(P<0.05).WPD,ED,and EVD had higher infection reduction rates than KPD,with ratios of 0.14-0.25,0.10-0.19,and 0.08-0.17,respectively,over 1-5 years(P<0.001).Continuous health education over 1-5 years showed that increasing coverage from a 10%baseline led to enhancing cost-effectiveness and intervention outcomes.Conclusions In high-endemic areas(infection rate≥20%)in China,prioritize WPD for better cost-effectiveness and outcomes.In medium-endemic areas(5%≤infection rate<20%)where WPD isn't feasible,use ED for cost-effectiveness and KPD for infection reduction,based on local needs.In low-endemic areas(infection rate<5%),encourage voluntary examination and treatment due to limited cost-effectiveness of mass treatment.Combining drug treatment with extensive health education can enhance long-term control effect.This strategy can guide control efforts for hookwormdiseases in China.
文摘In the field of landscape epidemiology,the contribution of machine learning(ML)to modeling of epidemiological risk scenarios presents itself as a good alternative.This study aims to break with the”black box”paradigm that underlies the application of automatic learning techniques by using SHAP to determine the contribution of each variable in ML models applied to geospatial health,using the prevalence of hookworms,intestinal parasites,in Ethiopia,where they are widely distributed;the country bears the third-highest burden of hookworm in Sub-Saharan Africa.XGBoost software was used,a very popular ML model,to fit and analyze the data.The Python SHAP library was used to understand the importance in the trained model,of the variables for predictions.The description of the contribution of these variables on a particular prediction was obtained,using different types of plot methods.The results show that the ML models are superior to the classical statistical models;not only demonstrating similar results but also explaining,by using the SHAP package,the influence and interactions between the variables in the generated models.This analysis provides information to help understand the epidemiological problem presented and provides a tool for similar studies.
文摘Objective To determine the length of protection by murine immunization with living third stage hookworm larvae (L 3) as measured by reduction in worm burden and host serologic antibody responses Methods Outbred male (Kunming strain) mice were immunized subcutaneously with 500 L 3 once every 2 weeks for a total of immunization for 3 times, and then challenged orally with 1000 L 3 for 1 to 8 weeks after the final immunization Host protective immunity was determined both by the reduction in worm burden as measured by the number of L 3 recovered from murine lungs 48 hour post challenge, as well as by measurement of circulating antibodies Histopathological responses were also examined Non immunized mice served as negative controls Results The protection by L 3 immunization declined over time One or 2 weeks after the final immunization, worm burdens were reduced 72% and 77 5% after challenge respectively In contrast, only 37% reduction in worm burden was observed when the L 3 challenge was delayed by 4 weeks and protection was almost entirely lost when there was an 8 week delay between the time of final immunization and challenge The reduced level of protection over time partially correlated with diminishing L 3 specific antibody responses Host inflammation in the lungs of immunized mice also diminished Conclusion The protection afforded by living L 3 immunization is maximal for the first two weeks after immunization, but then declines significantly over the ensuing
基金We are grateful to the staff at the Gushi County Center for Disease Control and Prevention,health workers at township hospitals and village clinics in the Gushi County,and to all participants who contributed their blood samples.This project was supported by the National Science&Technology Major Program(grant No.2012ZX10004-220).
文摘Background:Humoral and cellular immune responses play protective roles against Mycobacterium tuberculosis(MTB)infection.However,hookworm infection decreases the immune response to hookworm and bystander antigens.Currently,immune responses to co-infection of MTB and hookworm are still unknown,although co-infection has been one of the public health problems in co-endemic areas of pulmonary tuberculosis(PTB)and hookworm disease.Therefore,it is essential to evaluate B and T cell immune responses to the co-infection.Methods:Seventeen PTB cases co-infected with hookworm,26 PTB cases,15 patients with hookworm infection,and 24 healthy controls without PTB or hookworm infection were enrolled in the study.Expressions of CD3,CD4,CD8,CD10,CD19,CD20,CD21,CD25,CD27,CD38,FoxP3,and PD-1 were assessed on B and T cell subsets using multicolor flow cytometry.Results:For the B cell(CD19+)subsets,naïve B cells(CD10−CD27−CD21+CD20+),plasma cells(CD10−CD27+CD21−CD20−),and tissue-like memory B cells(CD10−CD27−CD21−CD20+)had higher proportions,whilst resting memory B cells(CD10−CD27+CD21+CD20+)had lower proportions in the group co-infected with MTB and hookworm as compared to other groups.Frequencies of activated memory B cells(CD10−CD27+CD21−CD20+)did not differ among the four groups.For the T cell(CD3+)subsets,frequencies of regulatory T cells(CD4+CD25+Foxp3+)and exhausted CD4+and CD8+T cells(CD4+PD-1+and CD8+PD-1+)were higher,and frequencies of activated CD4+and CD8+T cells(CD4+CD38+and CD8+CD38+)were lower in the co-infected group as compared to the other groups.Conclusion:The change patterns of the cell profile of circulating lymphocytes were indentified in human co-infection of MTB and hookworm,which might indicate that the humoral and cellular immune responses are more suppressed.
文摘Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Caninum) , were prepared as the source of antigen. Enzyme linked immunoelectrotransfer blotting (EITB) was enployed as an immunodiagnostic method. Results Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm infected patients (serum dilution 1∶200; antigen centrifuged at 36 000 r/m for 20 minutes, but not by sera from negative controls. Conclusion The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.
文摘Dear Editor,Hookworm is an important tropical nematode.The worm is a roundworm that can be seen in many tropical areas.Basically,this roundworm can cause gastrointestinal infection and chronic infection and result in hypochromic microcytic anemia,which is the common public health problem.Focusing on the worm’s life cycle,the hookworm egg will be passed from intestine of infected host and hatched as larva in the soil.Based on this information,bearfooted walking is at risk.In general,hookworm is a geohelminth.
