Ensuring national food security amidst rapid population growth and increasing extreme weather events remains a critical global challenge.However,the extent to which agricultural modernization in China enhances grain y...Ensuring national food security amidst rapid population growth and increasing extreme weather events remains a critical global challenge.However,the extent to which agricultural modernization in China enhances grain yield and contributes to food security remains unclear.Therefore,using panel data from 327 Chinese cities(2013–2021),this study employs spatial econometric models to analyze the spatial spillover effects of agricultural modernization level(AML)on grain yield and to reveal regional heterogeneity across nine major agricultural zones.The results showed a cumulative grain yield increase of 23.7 million tons,with peak productivity concentrated along the Hu Line and declining eastward and westward.AML also exhibited a steady increase but a clear spatial gradient,decreasing from coastal to inland regions,with the highest level observed in Southern China(SC).A key finding was that a 1%increase in AML directly raised local grain yield by an average of 4.185%,accompanied by significant positive spillover effects on neighboring regions.Regional variations revealed distinct patterns:the direct effects of AML were more pronounced in southern and eastern zones,while spillover effects dominated in northern and western zones.The largest positive direct impact of AML on grain yield was observed in the SC(8.499%),while Middle-Lower Yangtze Plain ranked second but exhibited the strongest positive spatial spillover effect(4.534%).These findings highlight the critical role of agricultural modernization in promoting grain production and provide a solid basis for optimizing regional agricultural systems,ensuring food security,and advancing sustainable agriculture.展开更多
Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to h...Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to humans,and the International Agency for Research on Cancer has classified it as a Group I carcinogen.Cadmium undergoes minimal metabolism in the human body;consequently,prolonged Cd^(2+)exposure can cause severe damage to multiple organs including the liver,kidneys,lungs,bones,and immune system(Shao et al.2024).Rice,one of the three global staple crops,and Cd exposure in humans primarily occurs the consumption of contaminated rice grains.The contribution of rice to the total dietary Cd intake is over 50% for non-smoking Asian populations(Chen et al.2018;Shi et al.2020).展开更多
Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergisticall...Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergistically regulating yield and quality remain unclear.Here,we report that the plasma membrane-localized transporter OsAAP18,which is more highly expressed in indica than japonica rice,positively correlates with tiller number and yield but negatively with grain width.OsAAP18 transports eight amino acids,including asparagine(Asn),proline(Pro),leucine(Leu),and valine(Val).Its overexpression increases yield through enhanced tillering and grain number per panicle while also improving rice processing and cooking quality.Transcriptome analysis showed that OsAAP18 coordinates grain development and quality formation by regulating the expression of key genes involved in starch and sucrose metabolism,nitrogen metabolism,and plant hormone signaling pathways.These findings establish OsAAP18 as a dual-function regulator that synergistically enhances yield and quality,offering a promising target for rice breeding.展开更多
Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassi...Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.展开更多
Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX...Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX2.While the use of elite Gn1a alleles has been well documented in indica rice cultivars,their potential in japonica rice remains largely unexplored.In this study,we characterized three suppressor mutants of the rice cytokinin receptor mutant pal1/ohk4 and found that all causal genes were novel alleles of Gn1a identified through the MutMap approach.These three suppressor mutants caused single amino acid substitutions in the FAD-binding domain(G556D and G156D)and the cytokinin-binding domain(Y357C),resulting in significantly reduced enzymatic activity of OsCKX2 and elevated cytokinin levels in the panicle.Haplotype analysis of Gn1a using a natural population from the 3K Rice Genomes Project showed that G556D,G156D,and Y357C were novel alleles of Gn1a.G556,G156,and Y357 were highly conserved,whereas four natural variants G54A,A105V,H116R,and N535K identified in different haplotypes of Gn1a showed extremely low conservation.By backcrossing the suppressor mutants with their original wild-type Huaidao 5,an elite japonica rice variety,we developed improved lines carrying only the gn1a mutation.The improved lines showed a significant increase in grain number per panicle,grain weight,panicle number per plant,plant height,and stem thickness,leading to a 25.7%-28.7%increase in grain yield per plot compared with Huaidao 5.This study provides valuable Gn1a alleles for synergistic improvement of the three key yield factors and offers germplasm resources for high-yielding breeding in japonica rice.展开更多
Increasing the grain yield(GY) and water use efficiency(WUE) of winter wheat in the Huaibei Plain(HP), China are essential. However, the effects of micro-sprinkler irrigation and topsoil compaction after wheat seed so...Increasing the grain yield(GY) and water use efficiency(WUE) of winter wheat in the Huaibei Plain(HP), China are essential. However, the effects of micro-sprinkler irrigation and topsoil compaction after wheat seed sowing on the GY and WUE are unclear. Therefore, a two-year field experiment was conducted during the 2021–2023 winter wheat growing seasons with a total six treatments: rain-fed(RF), conventional irrigation(CI) and micro-sprinkler irrigation(MI), as well as topsoil compaction after seed sowing under these three irrigation methods(RFC, CIC, and MIC). The results in the two years indicated that MI significantly increased GY compared to CI and RF, by averages of 17.9 and 42.1%, respectively. The increase in GY of MI was due to its significant increases in the number of spikes, kernels per spike, and grain weight. The chlorophyll concentration in flag leaves of MI after the anthesis stage maintained higher levels than with CI and RF, and was the lowest in RF. This was due to the dramatically enhanced catalase and peroxidase activities and lower malondialdehyde content under MI. Compared with RF and CI, MI significantly promoted dry matter remobilization and production after anthesis, as well as its contribution to GY. In addition, MI significantly boosted root growth, and root activity during the grain-filling stage was remarkably enhanced compared to CI and RF. In 2021–2022, there was no significant difference in WUE between MI and RF, but the WUE of RF was significantly lower than that of MI in 2022–2023. However, the WUE in MI was significantly improved compared to CI, and it increased by averages of 15.1 and 17.6% for the two years. Topsoil compaction significantly increased GY and WUE under rain-fed conditions due to improved spike numbers and dry matter production. Overall, topsoil compaction is advisable for enhancing GY and WUE in rain-fed conditions, whereas micro-sprinkler irrigation can be adopted to simultaneously achieve high GY and WUE in the HP.展开更多
Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low...Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low nitrogen inputs(Xu et al.,2012;Hu et al.,2023).However,modern cultivars are typically bred for high yields through excessive nitrogen fertilizer use,leading to the loss of beneficial alleles associated with high NUE during the breeding process(Wang and Peng,2017;Hu et al.,2023).Genetic improvement for high NUE should be a key strategy in breeding“Green Super Rice”(GSR)(Yu et al.,2021)and water-saving and drought-resistance rice(WDR)(Luo,2010;Xia et al.,2022)for sustainable agriculture.Asian cultivated rice is highly diverse and harbors vital genetic variants essential for adaptation to different environments(Wing et al.,2018).展开更多
Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while bala...Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.展开更多
Wheat is an important cereal crop used to produce diverse and popular food worldwide because of its high grain yield(GY)and grain protein content(GPC).However,GY and GPC are usually negatively correlated.We previously...Wheat is an important cereal crop used to produce diverse and popular food worldwide because of its high grain yield(GY)and grain protein content(GPC).However,GY and GPC are usually negatively correlated.We previously reported that favorable alleles of the wheat domestication gene Q can synchronously increase GY and GPC,but the underlying mechanisms remain largely unknown.In this study,we investigate the regulatory network involving Q associated with GY and GPC in young grains through DNA affinity purification sequencing and transcriptome sequencing analyses,electrophoretic mobility shift and dualluciferase assays,and transgenic approaches.Three Q-binding motifs,namely TTAAGG,AAACA[A/T]A,and GTAC[T/G]A,are identified.Notably,genes related to photosynthesis or carbon and nitrogen metabolism are enriched and regulated by Q.Moreover,Q is revealed to bind directly to its own gene and the glutamine synthetase gene GSr-4D to increase expression,thereby influencing nitrogen assimilation during the grain filling stage and increasing GPC.Considered together,our findings provide molecular evidence of the positive regulatory effects of Q on wheat GY and GPC.展开更多
Temperate forests exert significant biogeophysical influences on local and regional climates through modulating the energy and moisture exchanges between the land surface and the atmosphere,thereby serving as crucial ...Temperate forests exert significant biogeophysical influences on local and regional climates through modulating the energy and moisture exchanges between the land surface and the atmosphere,thereby serving as crucial barriers with significant buffering impacts on the productivity of adjacent agricultural ecosystems.However,the extent and underlying mechanisms of these biogeophysical and buffering effects of temperate forest barriers remains insufficiently understood.In this study,we integrated the dynamic crop model Noah-MP-Crop with the Weather Research and Forecasting(WRF)model to investigate the biogeophysical climate regulation of temperate forests and its buffering effects on crop yields in adjacent agricultural lands across Northeast China.Our findings revealed that temperate forest barriers induced significant local climate effects by cooling air and surface temperatures and reducing wind speeds within forested areas during the growing season,while also regulating non-local climate,particularly by altering regional precipitation patterns,2 m water vapor mixing ratio(Q2),and soil moisture,predominantly in adjacent cropland areas.Furthermore,these forest barriers were found to modulate climate extremes,through affecting maximum temperature and wind speed on a local scale,as well as both maximum and minimum Q2 in non-local croplands.Our study also observed that temperate forest barriers,through biogeophysical climate regulation,enhanced GPP,NPP,and grain yields across most cropland areas.This productivity boost was especially pronounced,with yield increases up to 20%in certain regions during the extreme drought conditions of 2017,underscoring the critical role of temperate forest barriers in sustaining and enhancing crop yields under severe climatic stress.Our findings underscore the significant buffering effects of temperate forest barriers on regional agricultural production,having important implications for climate adaptation strategies aimed at bolstering agricultural resilience in the face of increasing climate variability and extremes.展开更多
In this study,in order to analyze the multiple factors affecting grain production in Sichuan Province,grain production data and its related variables from 1982 to 2022 were selected as the object of the study and empi...In this study,in order to analyze the multiple factors affecting grain production in Sichuan Province,grain production data and its related variables from 1982 to 2022 were selected as the object of the study and empirical research was conducted.The unit root test and cointegration test were applied to ensure the smoothness of the data,heteroskedasticity and autocorrelation tests were performed,and the model was adjusted to finally select a feasible generalized least squares model.The results show that all the examined influencing factors have a significant impact on grain yield in Sichuan Province,especially the sown area of grain crops,which has the most significant effect.Based on these findings,this study proposes the following recommendations:ensure sufficient sown area and high-quality arable land resources,accelerate the process of agricultural mechanization and intelligence,and use chemical fertilizers rationally,in order to enhance the grain production capacity of Sichuan Province and guarantee food security.展开更多
Late sowing is a critical factor that hinders achieving high-yield,good-quality wheat under rice-wheat rotation.Understanding the physiological basis and regulatory pathways that lead to high yield and sound quality l...Late sowing is a critical factor that hinders achieving high-yield,good-quality wheat under rice-wheat rotation.Understanding the physiological basis and regulatory pathways that lead to high yield and sound quality late-sown wheat is crucial for developing effective cultivation strategies.A 2-year field experiment was conducted to investigate the effects of sowing date,nitrogen(N)application rate,and planting density on wheat yield,grain quality,population characteristics,and the underlying physiological factors.The results revealed significant interactions among the sowing date,planting density,and N application in regulating both yield and quality.Late sowing reduced grain yield primarily by reducing the number of spikes and kernels.However,the latter was improved by increasing N application and the planting density,thus mitigating the yield losses caused by late sowing.Moreover,the grain protein content(GPC)and wet gluten content(WGC)increased with delayed sowing dates and higher N rates but decreased with increased planting densities.For wheat yields over 9,000 or 7,500 kg ha^(-1),the latest sowing date should not be later than Nov.4 or 15,respectively.In addition,specific criteria should be met,including a maximum of 1.5 and 1.0 million stems and tillers ha^(-1),a maximum leaf area index of 6.7 and 5.5,and a dry matter accumulation(DMA)at anthesis of 14,000 and 12,000 kg ha^(-1),respectively.For high-yield,good-quality late-sown wheat,the optimal combination is a 25%increase in the N rate(300 kg N ha^(-1))and a planting density of 2.25 million(N300D225)or 3.75 million(N300D375)plants ha^(-1)for 10-or 20-day delays in sowing,respectively.These combinations result in a higher leaf net photosynthetic rate,higher activities of leaf nitrate reductase,glutamine synthetase,grain glutamic pyruvic transaminase,and a lower sugar-N ratio during post-anthesis.展开更多
Sorghum is an important cereal crop for smallholder farmers’ food security in many countries in West Africa. However, its production has stagnated due to several factors, such as anthracnose and grain molds. Thus, a ...Sorghum is an important cereal crop for smallholder farmers’ food security in many countries in West Africa. However, its production has stagnated due to several factors, such as anthracnose and grain molds. Thus, a study was conducted to identify local germplasms that combine high grain yield and resistance to anthracnose and grain molds under Senegalese environments. A set of 256 genotypes was assessed at Sefa, Sinthiou and Kolda research stations using an incomplete blocks design with two replications. Agro-morphological and phytopathological data were collected. The results revealed a huge phenotypic variation between the genotypes for all traits. The flowering time varied from 43 to 126 days after sowing, while the panicle length varied from 10 to 60 cm. The genotypes were generally more productive at Sinthiou (1653 Kg ha−1) compared to Kolda (164 kg ha−1) research stations. The disease parameters were significantly and positively associated, while the flowering time was strongly and positively associated to grain mold score. The genotypes were classified into three groups with plant height, panicle diameter and length, flowering time and grain mold score as the most discriminating parameters. The genotypes belonging to cluster 3, in addition of being more productive and more resistant to grain mold and anthracnose, have longer panicles. These genotypes present promising prospects for inclusion in breeding programs focused on advancing sorghum yield and disease resistance in Senegal.展开更多
Rice grain size and chalkiness are important traits that influence grain yield and quality,respectively.Mining of genes for grain yield and appearance quality and clarification of their action modes are of great impor...Rice grain size and chalkiness are important traits that influence grain yield and quality,respectively.Mining of genes for grain yield and appearance quality and clarification of their action modes are of great importance in rice breeding.In this study,a rice protein disulfide isomerase-like enzyme PDIL2-3 was characterized.Expression analysis revealed that PDIL2-3 was highly expressed in endosperm and spikelet hulls.The PDIL2-3-cri lines generated by CRISPR/Cas9 technology exhibited a chalky grain phenotype with altered storage substance accumulation and increased grain size and weight,whereas exactly opposite results were obtained for PDIL2-3 overexpression lines.Cytological experiments revealed that PDIL2-3-cri increased rice seed length mainly by increasing the cell number and rice seed width mainly by increasing the cell size in grains,implying that PDIL2-3 regulates the grain size by influencing both cell division and expansion of spikelet hulls.Further flow cytometric analysis validated that PDIL2-3 has a negative effect on cell proliferation,preventing DNA duplication and cell division in spikelet hulls.Moreover,q RT-PCR results showed that the expression levels of genes related to cell cycle and storage substance synthesis were significantly changed in PDIL2-3-cri transgenic lines.Thus,our results indicated that PDIL2-3 plays a pivotal role in influencing grain size and quality of rice by affecting cell division/expansion and storage substance accumulation,providing new insights into the function of PDIL family members in rice and enriching the genetic resources for rice breeding.展开更多
Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespira...Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespiration,a photorespiratory bypass consisting of Chlamydomonas reinhardtii glycolate dehydrogenase and Cucurbita maxima malate synthase(termed the GMS bypass)was introduced into the rice cultivar Zhonghua 11 and osplgg1b,a mutant of the rice chloroplast glycolate transporter,to generate GMS/ZH11 and GMS/osplgg1b transgenic plants.The GMS bypass reduced photorespiration and increased photosynthesis in the transgenic plants.The straw biomass of GMS/ZH11 and GMS/osplgg1b increased by up to 16.0%and 85.7%,respectively.The yield of GMS/ZH11 increased by 22.0%–34.7%in paddy fields.Thus,the GMS bypass can increase photosynthetic efficiency and yield in rice.展开更多
In maize production,the development of density-tolerant and lodging-resistant varieties has made dense planting an effective strategy for achieving high and stable yields,with superior hybrids serving as a prerequisit...In maize production,the development of density-tolerant and lodging-resistant varieties has made dense planting an effective strategy for achieving high and stable yields,with superior hybrids serving as a prerequisite for successful highdensity cultivation.However,the photosynthetic mechanisms underlying improved density tolerance in maize hybrids released across different eras in China remain unclear.This study investigates 40 years of breeding progress toward enhanced photosynthetic traits under varying planting densities and elucidates the physiological and ecological bases of improved density tolerance in maize hybrids.A three-year field experiment was conducted from 2019 to 2021 to compare eight major Chinese hybrids from four decadal cohorts under three planting densities:45,000(D1),67,500(D2),and 90,000(D3)plants ha^(-1).At high density(D3),modern hybrids exhibited a more optimal canopy architecture and superior leaf photosynthetic performance compared to older hybrids,despite a slight reduction in specific leaf nitrogen.Notably,modern hybrids(2000s)were able to maintain higher net photosynthetic rates and photosynthetic nitrogen use efficiency(PNUE)at D3,resulting in the highest grain yield(GY),which was 118.47%greater than that of older hybrids(1970s).Leaf area duration after anthesis,total chlorophyll content,key photosynthetic enzyme activities,and maximum quantum efficiency of PSII photochemistry were all positively correlated with GY.Among these,PNUE showed the strongest correlation with grain yield and thus represents a key indicator for optimizing maize hybrids.Based on these findings,breeders should continue selecting hybrids under high-density and suboptimal conditions,focusing on optimizing population architecture and enhancing photosynthetic capacity while fine-tuning leaf nitrogen status to develop high-yielding,density-tolerant hybrids capable of sustaining long-term increases in maize grain yield.展开更多
Irrigation methods and nitrogen(N) fertilization modes have complicated impacts on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality. However, the optimal water-N co...Irrigation methods and nitrogen(N) fertilization modes have complicated impacts on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality. However, the optimal water-N combination for drip-irrigated winter wheat remains unclear. A two-year field study was conducted to evaluate the influences of various N-fertigation and water regimes on wheat post-anthesis grain weight variation, yield, grain NPK content, and grain quality. The two irrigation quotas were I_(45)(irrigation when crop evapotranspiration reduced by effective rainfall(ETa-P) reaches 45 mm) and I_(30)(irrigation when ETa-P reaches 30 mm), while the six N application rates were N_(0–100)(100% at jointing/booting), N_(25–75)(25% at sowing and 75% at jointing/booting), N_(50–50)(50% at sowing and 50% at jointing/booting), N_(75–25)(75% at sowing and 25% at jointing/booting), N_(100–0)(100% at sowing), and SRF100(100% of slow-release fertilizer at sowing). The experimental findings showed that post-anthesis grain weight variation, grain yield, grain NPK content, and grain quality were all markedly influenced by the various irrigation schedules and N-fertilization modes. The N_(50–50)treatment was more beneficial for winter wheat post-anthesis grain weight variation than the N_(100–0)and N_(0–100)treatments under the two irrigation quotas and during the two seasons. The highest grain yields of 9.72 and9.94(t ha^(-1)) were obtained with the I_(45)N_(50–50)treatment in 2020–2021 and 2021–2022, respectively. The grain crudeprotein was higher in the I_(45)SRF100treatment during the two seasons. The I_(45)N_(100–0)combination significantly(P<0.05)enhanced the content of grain total starch by 7.30 and 8.23% compared with the I_(45)N_(0–100)and I_(30)N_(0–100)treatments,respectively, during the 2021–2021 season. The I_(45)N_(100–0)treatment significantly(P<0.05) enhanced the content ofgrain total starch concentration by 7.77, 7.62 and 7.88% compared with the I_(45)N_(0–100), I_(30)N_(0–100), and I_(30)N_(25–75)treatments,respectively, in the 2021–2022 season. The principal component analysis(PCA) indicated that the N_(50–50)splitN-fertigation mode could be the optimal choice for farmers during winter wheat production via drip irrigation.展开更多
Diversifying crop rotation aims to balance production and ecological concerns.However,yield and water use efficiency(WUE)of crop in diversified rotation systems have not been well documented,especially under limited i...Diversifying crop rotation aims to balance production and ecological concerns.However,yield and water use efficiency(WUE)of crop in diversified rotation systems have not been well documented,especially under limited irrigation.Here,we conducted a 6-year experiment with five treatments:1)wheatmaize cropping system(WM),as control;2)WMME,spring maize→WM rotation;3)WMML,spring millet→WM rotation;4)WMMP,spring peanut→WM rotation;and 5)WMMS,spring soybean→WM rotation,to explore how diversified rotations affected yield and WUE of wheat.Results showed that approximately 60% higher precipitation during wheat growing season in Cycle 1(2015-2017)resulted in yield increases by 33.8%-55.7% compared to those in Cycle 2(2017-2019)and Cycle3(2019-2021).Grain yield and WUE of wheat were 16.7% and 9.6% higher in Cycle 1,81.5% and 86.8% higher in Cycle 2,and 56.1% and 78.7% higher in Cycle 3 on average in diversified rotations compared to those in WM,respectively.Further analysis revealed that spike number and aboveground biomass were the main contributors to the increments,which can be explained by the increased evapotranspiration during the middle-late wheat growth stages(e.g.,regreening,jointing,and anthesis)in diversified rotations.In general,diversified rotations enhanced synchronization of soil water supply with crop water demand by affecting the spatiotemporal dynamics of soil moisture under varied precipitation conditions,thereby increasing yield and WUE of wheat.Hence,diversified spring crops→WM rotations offer a sustainable and efficient strategy for enhancing wheat production and water conservation in dry areas.展开更多
Mechanical properties of Mg-3Gd(wt.%)samples with average grain sizes ranging from 3 to 45μm were characterized by room temperature tensile test.A reversal of the trade-off,i.e.,high yield strength and large tensile ...Mechanical properties of Mg-3Gd(wt.%)samples with average grain sizes ranging from 3 to 45μm were characterized by room temperature tensile test.A reversal of the trade-off,i.e.,high yield strength and large tensile elongation,was simultaneously observed in the fine-grained samples.The microstructures and hardening response were analyzed in terms of the viewpoint of strain evolution,including local strain evolution by tensile digital image correlation strain measurement,and lattice strain by using synchrotronbased in-situ high energy X-ray diffraction technique.The dislocation-based deformation mechanisms were investigated to underpin the microstructural origin of the yield point phenomenon and enhancement in work-hardening.The occurrence of the yield point phenomenon represented by a yield drop and propagation of the Lüders band is related to the absence of mobile dislocations at an early stage and to the slip transmission between the adjacent grain.The extraordinary work-hardening enhancement over an extended range can be ascribed mainly to the increases in dislocation multiplication and accumulation capabilities by the activation and interaction of multiple slip systems includingand<c+a>types.These results contribute to the design of strong and ductile Mg alloys.展开更多
The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-...The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-pyrroline(2-AP),which is significantly affected by field management measures.The purpose of this study was to investigate the impact of tillage management and straw return on the grain yield and biosynthesis of 2-AP in fragrant rice.This study was conducted over two years in 2016 and 2017 and used two fragrant rice cultivars(Meixiangzhan 2 and Xiangyaxiangzhan)as materials.The experimental design consisted of different tillage management and straw return treatments,which included three tillage management regimes:rotary tillage(T0),minimum tillage(T1),and no tillage(T2);and two straw return treatments:without straw return(S0)and straw return(S1).The straw used for the experiment was sourced from the residue of the corresponding fragrant rice cultivar harvested in the early season.Tillage management and straw return substantially affected the grain yields,grain quality,and 2-AP contents of both fragrant rice cultivars.Compared with the T0S0 treatment,tillage management and straw return resulted in 2-AP content improvements in 2016(12.41–116.85%)and 2017(34.85–103.89%)on average.Higher 2-AP contents were also detected in both fragrant rice cultivars in the T2S1 and T1S1 treatments.A structural equation model(SEM)demonstrated that the activities of enzymes related to fragrance metabolism in the leaves and grain jointly regulated the biosynthesis of precursors of fragrance metabolism in the grain,which further promoted the accumulation of 2-AP.In addition,a principal component analysis indicated that the T1S1 treatment was positively correlated with both 2-AP and grain yield.The SEM demonstrated that the enzymes related to nitrogen metabolism,parameters related to photosynthesis,and yield components contributed to the grain yield.The T1S1 treatment resulted in the highest average grain yield of 760.75 g m^(-2),which could be attributed to increases in various attributes,such as the leaf area index,SPAD value,nitrogen metabolism,panicle number m^(-2),and grain number per panicle.In summary,the minimum tillage and straw return(T1S1)treatment is more effective at simultaneously improving both the grain yield and 2-AP content in fragrant rice.展开更多
基金National Natural Science Foundation of China,No.42471455,No.42230113National Key Research and Development Program of China,No.2022YFC3800804-01。
文摘Ensuring national food security amidst rapid population growth and increasing extreme weather events remains a critical global challenge.However,the extent to which agricultural modernization in China enhances grain yield and contributes to food security remains unclear.Therefore,using panel data from 327 Chinese cities(2013–2021),this study employs spatial econometric models to analyze the spatial spillover effects of agricultural modernization level(AML)on grain yield and to reveal regional heterogeneity across nine major agricultural zones.The results showed a cumulative grain yield increase of 23.7 million tons,with peak productivity concentrated along the Hu Line and declining eastward and westward.AML also exhibited a steady increase but a clear spatial gradient,decreasing from coastal to inland regions,with the highest level observed in Southern China(SC).A key finding was that a 1%increase in AML directly raised local grain yield by an average of 4.185%,accompanied by significant positive spillover effects on neighboring regions.Regional variations revealed distinct patterns:the direct effects of AML were more pronounced in southern and eastern zones,while spillover effects dominated in northern and western zones.The largest positive direct impact of AML on grain yield was observed in the SC(8.499%),while Middle-Lower Yangtze Plain ranked second but exhibited the strongest positive spatial spillover effect(4.534%).These findings highlight the critical role of agricultural modernization in promoting grain production and provide a solid basis for optimizing regional agricultural systems,ensuring food security,and advancing sustainable agriculture.
基金financially supported by the National Key R&D Program of China(2024YFD1200800)the Guangdong Basic and Applied Basic Research Foundation,China(2024A1515030094)。
文摘Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to humans,and the International Agency for Research on Cancer has classified it as a Group I carcinogen.Cadmium undergoes minimal metabolism in the human body;consequently,prolonged Cd^(2+)exposure can cause severe damage to multiple organs including the liver,kidneys,lungs,bones,and immune system(Shao et al.2024).Rice,one of the three global staple crops,and Cd exposure in humans primarily occurs the consumption of contaminated rice grains.The contribution of rice to the total dietary Cd intake is over 50% for non-smoking Asian populations(Chen et al.2018;Shi et al.2020).
基金supported by the National Natural Science Foundation of China(Grant Nos.32560065 and 32572249)the Guizhou Provincial Excellent Young Talents Project of Science and Technology,China(Grant No.qiankehepingtairencai-YQK(2023)002)+4 种基金the Guizhou Provincial Science and Technology Projects,China(Grant Nos.qiankehechengguo(2024)General 116 and qiankehejichu-ZK(2022)Key 008)the Key Laboratory of High Quality,High Efficiency,and Yield Enhancement in Grain and Oil Crops,China(Grant No.Qiankehe-Platform ZSYS(2025)037)the Key Laboratory of Functional Agriculture of Guizhou Provincial Department of Education,China(Grant No.Qianjiaoji(2023)007)Guizhou Provincial Modern Agricultural Industry Technology System Construction Special Program(Grant No.GZSDCYJSTX-202602)the Qiandongnan Science and Technology Support Project,China(Grant No.Qiandongnan Kehe Support(2023)07).
文摘Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergistically regulating yield and quality remain unclear.Here,we report that the plasma membrane-localized transporter OsAAP18,which is more highly expressed in indica than japonica rice,positively correlates with tiller number and yield but negatively with grain width.OsAAP18 transports eight amino acids,including asparagine(Asn),proline(Pro),leucine(Leu),and valine(Val).Its overexpression increases yield through enhanced tillering and grain number per panicle while also improving rice processing and cooking quality.Transcriptome analysis showed that OsAAP18 coordinates grain development and quality formation by regulating the expression of key genes involved in starch and sucrose metabolism,nitrogen metabolism,and plant hormone signaling pathways.These findings establish OsAAP18 as a dual-function regulator that synergistically enhances yield and quality,offering a promising target for rice breeding.
基金funded by the National Natural Science Foundation of China (31760363)the Earmarked Fund for CARS (CARS-14-1-16)+1 种基金the Gansu Education Science and Technology Innovation Industry Support Program,China (2021CYZC-38)the Gansu Provincial Key Laboratory of Arid Land Crop Science,Gansu Agricultural University,China (GSCS-2020-Z6)。
文摘Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.
基金supported by the Biological Breeding-National Science and Technology Major Project,China(Grant No.2023ZD0406801)the National Natural Science Foundation of China(Grant No.32300278)+2 种基金the Key R&D Plan of Shandong Province,China(Grant No.2024LZGC009)the Innovation Program of Chinese Academy of Agricultural Sciences(Grant No.CAAS-CSCB-202402)the Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences,China(Grant No.CXGC2025B09).
文摘Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX2.While the use of elite Gn1a alleles has been well documented in indica rice cultivars,their potential in japonica rice remains largely unexplored.In this study,we characterized three suppressor mutants of the rice cytokinin receptor mutant pal1/ohk4 and found that all causal genes were novel alleles of Gn1a identified through the MutMap approach.These three suppressor mutants caused single amino acid substitutions in the FAD-binding domain(G556D and G156D)and the cytokinin-binding domain(Y357C),resulting in significantly reduced enzymatic activity of OsCKX2 and elevated cytokinin levels in the panicle.Haplotype analysis of Gn1a using a natural population from the 3K Rice Genomes Project showed that G556D,G156D,and Y357C were novel alleles of Gn1a.G556,G156,and Y357 were highly conserved,whereas four natural variants G54A,A105V,H116R,and N535K identified in different haplotypes of Gn1a showed extremely low conservation.By backcrossing the suppressor mutants with their original wild-type Huaidao 5,an elite japonica rice variety,we developed improved lines carrying only the gn1a mutation.The improved lines showed a significant increase in grain number per panicle,grain weight,panicle number per plant,plant height,and stem thickness,leading to a 25.7%-28.7%increase in grain yield per plot compared with Huaidao 5.This study provides valuable Gn1a alleles for synergistic improvement of the three key yield factors and offers germplasm resources for high-yielding breeding in japonica rice.
基金funding from the Scientific Research Program of the Higher Educational Institutions in Anhui Province, China (2023AH050986)the Natural Science Foundation of Anhui Province, China (240805MC063)+1 种基金the National Natural Science Foundation of China (32172119)the Talent Introduction Project of Anhui Agricultural University, China (rc312212 and yj2019-01)。
文摘Increasing the grain yield(GY) and water use efficiency(WUE) of winter wheat in the Huaibei Plain(HP), China are essential. However, the effects of micro-sprinkler irrigation and topsoil compaction after wheat seed sowing on the GY and WUE are unclear. Therefore, a two-year field experiment was conducted during the 2021–2023 winter wheat growing seasons with a total six treatments: rain-fed(RF), conventional irrigation(CI) and micro-sprinkler irrigation(MI), as well as topsoil compaction after seed sowing under these three irrigation methods(RFC, CIC, and MIC). The results in the two years indicated that MI significantly increased GY compared to CI and RF, by averages of 17.9 and 42.1%, respectively. The increase in GY of MI was due to its significant increases in the number of spikes, kernels per spike, and grain weight. The chlorophyll concentration in flag leaves of MI after the anthesis stage maintained higher levels than with CI and RF, and was the lowest in RF. This was due to the dramatically enhanced catalase and peroxidase activities and lower malondialdehyde content under MI. Compared with RF and CI, MI significantly promoted dry matter remobilization and production after anthesis, as well as its contribution to GY. In addition, MI significantly boosted root growth, and root activity during the grain-filling stage was remarkably enhanced compared to CI and RF. In 2021–2022, there was no significant difference in WUE between MI and RF, but the WUE of RF was significantly lower than that of MI in 2022–2023. However, the WUE in MI was significantly improved compared to CI, and it increased by averages of 15.1 and 17.6% for the two years. Topsoil compaction significantly increased GY and WUE under rain-fed conditions due to improved spike numbers and dry matter production. Overall, topsoil compaction is advisable for enhancing GY and WUE in rain-fed conditions, whereas micro-sprinkler irrigation can be adopted to simultaneously achieve high GY and WUE in the HP.
基金supported by Joint Funds of National Natural Science Foundation of China(U24A20399)Natural Science Foundation of Shanghai(23JC1403500,22ZR1455300)+2 种基金Specific university discipline construction project(2023B10564002,2023B10564004)Shanghai Agricultural Science and Technology Innovation Program(2024-02-08-00-12-F00028)Earmarked Fund for China Agriculture Research System(CARS-01).
文摘Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low nitrogen inputs(Xu et al.,2012;Hu et al.,2023).However,modern cultivars are typically bred for high yields through excessive nitrogen fertilizer use,leading to the loss of beneficial alleles associated with high NUE during the breeding process(Wang and Peng,2017;Hu et al.,2023).Genetic improvement for high NUE should be a key strategy in breeding“Green Super Rice”(GSR)(Yu et al.,2021)and water-saving and drought-resistance rice(WDR)(Luo,2010;Xia et al.,2022)for sustainable agriculture.Asian cultivated rice is highly diverse and harbors vital genetic variants essential for adaptation to different environments(Wing et al.,2018).
基金he National Key Research and Development Program of China(2022YFD2300304)the National Natural Science Foundation of China(31671617)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.
基金supported by the National Key Research and Development Program of China(2023YFD1200404)the Science and Technology Department of Sichuan Province(2024ZYD0160)the National Natural Science Foundation of China(32072054).
文摘Wheat is an important cereal crop used to produce diverse and popular food worldwide because of its high grain yield(GY)and grain protein content(GPC).However,GY and GPC are usually negatively correlated.We previously reported that favorable alleles of the wheat domestication gene Q can synchronously increase GY and GPC,but the underlying mechanisms remain largely unknown.In this study,we investigate the regulatory network involving Q associated with GY and GPC in young grains through DNA affinity purification sequencing and transcriptome sequencing analyses,electrophoretic mobility shift and dualluciferase assays,and transgenic approaches.Three Q-binding motifs,namely TTAAGG,AAACA[A/T]A,and GTAC[T/G]A,are identified.Notably,genes related to photosynthesis or carbon and nitrogen metabolism are enriched and regulated by Q.Moreover,Q is revealed to bind directly to its own gene and the glutamine synthetase gene GSr-4D to increase expression,thereby influencing nitrogen assimilation during the grain filling stage and increasing GPC.Considered together,our findings provide molecular evidence of the positive regulatory effects of Q on wheat GY and GPC.
基金supported by National Key R&D Program of China(Grant No.2024YFD1501600)the National Natural Science Foundation of China(Grants No.42071025,42371075)the Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2023240).
文摘Temperate forests exert significant biogeophysical influences on local and regional climates through modulating the energy and moisture exchanges between the land surface and the atmosphere,thereby serving as crucial barriers with significant buffering impacts on the productivity of adjacent agricultural ecosystems.However,the extent and underlying mechanisms of these biogeophysical and buffering effects of temperate forest barriers remains insufficiently understood.In this study,we integrated the dynamic crop model Noah-MP-Crop with the Weather Research and Forecasting(WRF)model to investigate the biogeophysical climate regulation of temperate forests and its buffering effects on crop yields in adjacent agricultural lands across Northeast China.Our findings revealed that temperate forest barriers induced significant local climate effects by cooling air and surface temperatures and reducing wind speeds within forested areas during the growing season,while also regulating non-local climate,particularly by altering regional precipitation patterns,2 m water vapor mixing ratio(Q2),and soil moisture,predominantly in adjacent cropland areas.Furthermore,these forest barriers were found to modulate climate extremes,through affecting maximum temperature and wind speed on a local scale,as well as both maximum and minimum Q2 in non-local croplands.Our study also observed that temperate forest barriers,through biogeophysical climate regulation,enhanced GPP,NPP,and grain yields across most cropland areas.This productivity boost was especially pronounced,with yield increases up to 20%in certain regions during the extreme drought conditions of 2017,underscoring the critical role of temperate forest barriers in sustaining and enhancing crop yields under severe climatic stress.Our findings underscore the significant buffering effects of temperate forest barriers on regional agricultural production,having important implications for climate adaptation strategies aimed at bolstering agricultural resilience in the face of increasing climate variability and extremes.
文摘In this study,in order to analyze the multiple factors affecting grain production in Sichuan Province,grain production data and its related variables from 1982 to 2022 were selected as the object of the study and empirical research was conducted.The unit root test and cointegration test were applied to ensure the smoothness of the data,heteroskedasticity and autocorrelation tests were performed,and the model was adjusted to finally select a feasible generalized least squares model.The results show that all the examined influencing factors have a significant impact on grain yield in Sichuan Province,especially the sown area of grain crops,which has the most significant effect.Based on these findings,this study proposes the following recommendations:ensure sufficient sown area and high-quality arable land resources,accelerate the process of agricultural mechanization and intelligence,and use chemical fertilizers rationally,in order to enhance the grain production capacity of Sichuan Province and guarantee food security.
基金supported by the National Natural Science Foundation of China(32272215)the Key R&D Program of Jiangsu Province,China(BE2021361-1)the Collaborative Innovation Center for Modern Crop Production by Province and Ministry(CIC-MCP),Nanjing Agricultural University,China。
文摘Late sowing is a critical factor that hinders achieving high-yield,good-quality wheat under rice-wheat rotation.Understanding the physiological basis and regulatory pathways that lead to high yield and sound quality late-sown wheat is crucial for developing effective cultivation strategies.A 2-year field experiment was conducted to investigate the effects of sowing date,nitrogen(N)application rate,and planting density on wheat yield,grain quality,population characteristics,and the underlying physiological factors.The results revealed significant interactions among the sowing date,planting density,and N application in regulating both yield and quality.Late sowing reduced grain yield primarily by reducing the number of spikes and kernels.However,the latter was improved by increasing N application and the planting density,thus mitigating the yield losses caused by late sowing.Moreover,the grain protein content(GPC)and wet gluten content(WGC)increased with delayed sowing dates and higher N rates but decreased with increased planting densities.For wheat yields over 9,000 or 7,500 kg ha^(-1),the latest sowing date should not be later than Nov.4 or 15,respectively.In addition,specific criteria should be met,including a maximum of 1.5 and 1.0 million stems and tillers ha^(-1),a maximum leaf area index of 6.7 and 5.5,and a dry matter accumulation(DMA)at anthesis of 14,000 and 12,000 kg ha^(-1),respectively.For high-yield,good-quality late-sown wheat,the optimal combination is a 25%increase in the N rate(300 kg N ha^(-1))and a planting density of 2.25 million(N300D225)or 3.75 million(N300D375)plants ha^(-1)for 10-or 20-day delays in sowing,respectively.These combinations result in a higher leaf net photosynthetic rate,higher activities of leaf nitrate reductase,glutamine synthetase,grain glutamic pyruvic transaminase,and a lower sugar-N ratio during post-anthesis.
文摘Sorghum is an important cereal crop for smallholder farmers’ food security in many countries in West Africa. However, its production has stagnated due to several factors, such as anthracnose and grain molds. Thus, a study was conducted to identify local germplasms that combine high grain yield and resistance to anthracnose and grain molds under Senegalese environments. A set of 256 genotypes was assessed at Sefa, Sinthiou and Kolda research stations using an incomplete blocks design with two replications. Agro-morphological and phytopathological data were collected. The results revealed a huge phenotypic variation between the genotypes for all traits. The flowering time varied from 43 to 126 days after sowing, while the panicle length varied from 10 to 60 cm. The genotypes were generally more productive at Sinthiou (1653 Kg ha−1) compared to Kolda (164 kg ha−1) research stations. The disease parameters were significantly and positively associated, while the flowering time was strongly and positively associated to grain mold score. The genotypes were classified into three groups with plant height, panicle diameter and length, flowering time and grain mold score as the most discriminating parameters. The genotypes belonging to cluster 3, in addition of being more productive and more resistant to grain mold and anthracnose, have longer panicles. These genotypes present promising prospects for inclusion in breeding programs focused on advancing sorghum yield and disease resistance in Senegal.
基金supported in part by grants from the National Natural Science Foundation of China(32472141,32101746,and 32102231)Natural Science Foundation of Hubei Province(JCZRLH202500540,2021CFB033,and 2022CFB393)。
文摘Rice grain size and chalkiness are important traits that influence grain yield and quality,respectively.Mining of genes for grain yield and appearance quality and clarification of their action modes are of great importance in rice breeding.In this study,a rice protein disulfide isomerase-like enzyme PDIL2-3 was characterized.Expression analysis revealed that PDIL2-3 was highly expressed in endosperm and spikelet hulls.The PDIL2-3-cri lines generated by CRISPR/Cas9 technology exhibited a chalky grain phenotype with altered storage substance accumulation and increased grain size and weight,whereas exactly opposite results were obtained for PDIL2-3 overexpression lines.Cytological experiments revealed that PDIL2-3-cri increased rice seed length mainly by increasing the cell number and rice seed width mainly by increasing the cell size in grains,implying that PDIL2-3 regulates the grain size by influencing both cell division and expansion of spikelet hulls.Further flow cytometric analysis validated that PDIL2-3 has a negative effect on cell proliferation,preventing DNA duplication and cell division in spikelet hulls.Moreover,q RT-PCR results showed that the expression levels of genes related to cell cycle and storage substance synthesis were significantly changed in PDIL2-3-cri transgenic lines.Thus,our results indicated that PDIL2-3 plays a pivotal role in influencing grain size and quality of rice by affecting cell division/expansion and storage substance accumulation,providing new insights into the function of PDIL family members in rice and enriching the genetic resources for rice breeding.
基金supported by the National Key Research and Development Program of China(2020YFA0907600)the Biological Breeding-National Science and Technology Major Project(2024ZD04080)+1 种基金the National Natural Science Foundation of China(32270252)the Natural Science Foundation of Guangdong Province(2024A1515011085).
文摘Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespiration,a photorespiratory bypass consisting of Chlamydomonas reinhardtii glycolate dehydrogenase and Cucurbita maxima malate synthase(termed the GMS bypass)was introduced into the rice cultivar Zhonghua 11 and osplgg1b,a mutant of the rice chloroplast glycolate transporter,to generate GMS/ZH11 and GMS/osplgg1b transgenic plants.The GMS bypass reduced photorespiration and increased photosynthesis in the transgenic plants.The straw biomass of GMS/ZH11 and GMS/osplgg1b increased by up to 16.0%and 85.7%,respectively.The yield of GMS/ZH11 increased by 22.0%–34.7%in paddy fields.Thus,the GMS bypass can increase photosynthetic efficiency and yield in rice.
基金supported by the National Natural Science Foundation of China(32071960)the National Key Research and Development Program of China(2018YFD0300603)。
文摘In maize production,the development of density-tolerant and lodging-resistant varieties has made dense planting an effective strategy for achieving high and stable yields,with superior hybrids serving as a prerequisite for successful highdensity cultivation.However,the photosynthetic mechanisms underlying improved density tolerance in maize hybrids released across different eras in China remain unclear.This study investigates 40 years of breeding progress toward enhanced photosynthetic traits under varying planting densities and elucidates the physiological and ecological bases of improved density tolerance in maize hybrids.A three-year field experiment was conducted from 2019 to 2021 to compare eight major Chinese hybrids from four decadal cohorts under three planting densities:45,000(D1),67,500(D2),and 90,000(D3)plants ha^(-1).At high density(D3),modern hybrids exhibited a more optimal canopy architecture and superior leaf photosynthetic performance compared to older hybrids,despite a slight reduction in specific leaf nitrogen.Notably,modern hybrids(2000s)were able to maintain higher net photosynthetic rates and photosynthetic nitrogen use efficiency(PNUE)at D3,resulting in the highest grain yield(GY),which was 118.47%greater than that of older hybrids(1970s).Leaf area duration after anthesis,total chlorophyll content,key photosynthetic enzyme activities,and maximum quantum efficiency of PSII photochemistry were all positively correlated with GY.Among these,PNUE showed the strongest correlation with grain yield and thus represents a key indicator for optimizing maize hybrids.Based on these findings,breeders should continue selecting hybrids under high-density and suboptimal conditions,focusing on optimizing population architecture and enhancing photosynthetic capacity while fine-tuning leaf nitrogen status to develop high-yielding,density-tolerant hybrids capable of sustaining long-term increases in maize grain yield.
基金supported by the China Agriculture Research System of MOF and MARA (CARS-03-19)the National Natural Science Foundation of China (51879267)the Agricultural Science and Technology Innovation Program (ASTIP), Chinese Academy of Agricultural Sciences。
文摘Irrigation methods and nitrogen(N) fertilization modes have complicated impacts on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality. However, the optimal water-N combination for drip-irrigated winter wheat remains unclear. A two-year field study was conducted to evaluate the influences of various N-fertigation and water regimes on wheat post-anthesis grain weight variation, yield, grain NPK content, and grain quality. The two irrigation quotas were I_(45)(irrigation when crop evapotranspiration reduced by effective rainfall(ETa-P) reaches 45 mm) and I_(30)(irrigation when ETa-P reaches 30 mm), while the six N application rates were N_(0–100)(100% at jointing/booting), N_(25–75)(25% at sowing and 75% at jointing/booting), N_(50–50)(50% at sowing and 50% at jointing/booting), N_(75–25)(75% at sowing and 25% at jointing/booting), N_(100–0)(100% at sowing), and SRF100(100% of slow-release fertilizer at sowing). The experimental findings showed that post-anthesis grain weight variation, grain yield, grain NPK content, and grain quality were all markedly influenced by the various irrigation schedules and N-fertilization modes. The N_(50–50)treatment was more beneficial for winter wheat post-anthesis grain weight variation than the N_(100–0)and N_(0–100)treatments under the two irrigation quotas and during the two seasons. The highest grain yields of 9.72 and9.94(t ha^(-1)) were obtained with the I_(45)N_(50–50)treatment in 2020–2021 and 2021–2022, respectively. The grain crudeprotein was higher in the I_(45)SRF100treatment during the two seasons. The I_(45)N_(100–0)combination significantly(P<0.05)enhanced the content of grain total starch by 7.30 and 8.23% compared with the I_(45)N_(0–100)and I_(30)N_(0–100)treatments,respectively, during the 2021–2021 season. The I_(45)N_(100–0)treatment significantly(P<0.05) enhanced the content ofgrain total starch concentration by 7.77, 7.62 and 7.88% compared with the I_(45)N_(0–100), I_(30)N_(0–100), and I_(30)N_(25–75)treatments,respectively, in the 2021–2022 season. The principal component analysis(PCA) indicated that the N_(50–50)splitN-fertigation mode could be the optimal choice for farmers during winter wheat production via drip irrigation.
基金supported by the National Natural Science Foundation of China(32172125 and U21A20218)。
文摘Diversifying crop rotation aims to balance production and ecological concerns.However,yield and water use efficiency(WUE)of crop in diversified rotation systems have not been well documented,especially under limited irrigation.Here,we conducted a 6-year experiment with five treatments:1)wheatmaize cropping system(WM),as control;2)WMME,spring maize→WM rotation;3)WMML,spring millet→WM rotation;4)WMMP,spring peanut→WM rotation;and 5)WMMS,spring soybean→WM rotation,to explore how diversified rotations affected yield and WUE of wheat.Results showed that approximately 60% higher precipitation during wheat growing season in Cycle 1(2015-2017)resulted in yield increases by 33.8%-55.7% compared to those in Cycle 2(2017-2019)and Cycle3(2019-2021).Grain yield and WUE of wheat were 16.7% and 9.6% higher in Cycle 1,81.5% and 86.8% higher in Cycle 2,and 56.1% and 78.7% higher in Cycle 3 on average in diversified rotations compared to those in WM,respectively.Further analysis revealed that spike number and aboveground biomass were the main contributors to the increments,which can be explained by the increased evapotranspiration during the middle-late wheat growth stages(e.g.,regreening,jointing,and anthesis)in diversified rotations.In general,diversified rotations enhanced synchronization of soil water supply with crop water demand by affecting the spatiotemporal dynamics of soil moisture under varied precipitation conditions,thereby increasing yield and WUE of wheat.Hence,diversified spring crops→WM rotations offer a sustainable and efficient strategy for enhancing wheat production and water conservation in dry areas.
基金financially supported by the National Key Research and Develop-ment Program(No.2023YFB3712702)the National Natural Science Foundation of China(Nos.52071038,52071039,and 52301156)the Natural Science Foundation of Jiangsu Province(Nos.BK20232025 and BK20243005)。
文摘Mechanical properties of Mg-3Gd(wt.%)samples with average grain sizes ranging from 3 to 45μm were characterized by room temperature tensile test.A reversal of the trade-off,i.e.,high yield strength and large tensile elongation,was simultaneously observed in the fine-grained samples.The microstructures and hardening response were analyzed in terms of the viewpoint of strain evolution,including local strain evolution by tensile digital image correlation strain measurement,and lattice strain by using synchrotronbased in-situ high energy X-ray diffraction technique.The dislocation-based deformation mechanisms were investigated to underpin the microstructural origin of the yield point phenomenon and enhancement in work-hardening.The occurrence of the yield point phenomenon represented by a yield drop and propagation of the Lüders band is related to the absence of mobile dislocations at an early stage and to the slip transmission between the adjacent grain.The extraordinary work-hardening enhancement over an extended range can be ascribed mainly to the increases in dislocation multiplication and accumulation capabilities by the activation and interaction of multiple slip systems includingand<c+a>types.These results contribute to the design of strong and ductile Mg alloys.
基金funding provided by the National Natural Science Foundation of China(31971843 and 31271646)the Guangxi Natural Science Foundation,China(2021GXNSFBA196084)。
文摘The practice of conservation tillage or straw return to the farmland influences the grain yield and quality of rice(Oryza sativa).The key volatile compound responsible for the fragrance of fragrant rice is 2-acetyl-1-pyrroline(2-AP),which is significantly affected by field management measures.The purpose of this study was to investigate the impact of tillage management and straw return on the grain yield and biosynthesis of 2-AP in fragrant rice.This study was conducted over two years in 2016 and 2017 and used two fragrant rice cultivars(Meixiangzhan 2 and Xiangyaxiangzhan)as materials.The experimental design consisted of different tillage management and straw return treatments,which included three tillage management regimes:rotary tillage(T0),minimum tillage(T1),and no tillage(T2);and two straw return treatments:without straw return(S0)and straw return(S1).The straw used for the experiment was sourced from the residue of the corresponding fragrant rice cultivar harvested in the early season.Tillage management and straw return substantially affected the grain yields,grain quality,and 2-AP contents of both fragrant rice cultivars.Compared with the T0S0 treatment,tillage management and straw return resulted in 2-AP content improvements in 2016(12.41–116.85%)and 2017(34.85–103.89%)on average.Higher 2-AP contents were also detected in both fragrant rice cultivars in the T2S1 and T1S1 treatments.A structural equation model(SEM)demonstrated that the activities of enzymes related to fragrance metabolism in the leaves and grain jointly regulated the biosynthesis of precursors of fragrance metabolism in the grain,which further promoted the accumulation of 2-AP.In addition,a principal component analysis indicated that the T1S1 treatment was positively correlated with both 2-AP and grain yield.The SEM demonstrated that the enzymes related to nitrogen metabolism,parameters related to photosynthesis,and yield components contributed to the grain yield.The T1S1 treatment resulted in the highest average grain yield of 760.75 g m^(-2),which could be attributed to increases in various attributes,such as the leaf area index,SPAD value,nitrogen metabolism,panicle number m^(-2),and grain number per panicle.In summary,the minimum tillage and straw return(T1S1)treatment is more effective at simultaneously improving both the grain yield and 2-AP content in fragrant rice.
