Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-in...Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-induced apoptosis in HepG2 cells. HepG2 cells were incubated with germacrone for 24 h, the protein expression of p-STAT3, STAT3, p-JAK2 and JAK2 was detected by Westem Blotting, and RT-PCR was used to determine the expression of STAT3, p53, Bcl-2 and Bax at transcriptional levels. Besides that, HepG2 cells were pre-treated with AG490 or IL-6 for 2 h, and then incubated with ger- macrone for 24 h. The expression ofp-JAK2, JAK2, p-STAT3, STAT3, p53, Bax and Bcl-2 was detected by Western blotting. The activity of HepG2 cells was tested by MTT assay. The apoptosis of HepG2 cells and levels of reactive oxygen species （ROS） were flow cytometrically measured. The results showed that germacrone exposure decreased p-STAT3 and p-JAK2 and regulated expression of p53 and Bcl-2 family members at the same time. Moreover, IL-6 enhanced the activation of the JAK2/STAT3 signalling pathway and therefore attenuated the germacrone-induced apoptosis. Suppression of JAK2/STAT3 signalling pathway by AG490, an inhibitor of JAK2, resulted in apoptosis and an increase in ROS in response to germacrone exposure. We therefore conclude that germacrone induces apoptosis through the JAK2/STAT3 signalling pathway.展开更多
Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV de...Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detectionwas 100 ng·mL^(-1) for germacrone in plasma and the linear range was 0.1004-15.06 μg·mL^(-1) inplasma. The RSD of intra-day and inter-day assay was 1.87% - 4.29% and 1.29% -5.15%, respectively.The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show anyinterference in the analysis. Conclusion The method is accurate and convenient, and suitable forpharmacokinetic studies of germacrone in rats.展开更多
Curcumae Rhizoma,derived from the rhizome of Curcuma phaeocaulis,Curcuma kwangsiensis and Curcuma wenyujin,was called Ezhu in China.In the past,Curcumae Rhizoma extracts were obtained through water decoction or altern...Curcumae Rhizoma,derived from the rhizome of Curcuma phaeocaulis,Curcuma kwangsiensis and Curcuma wenyujin,was called Ezhu in China.In the past,Curcumae Rhizoma extracts were obtained through water decoction or alternative methods,which showed signifcant anti-cancer effects.However,the mixed extracts contain various compound components of Curcumae Rhizoma,leading to an ambiguous mechanism of action for Curcumae Rhizoma extracts anti-cancer.Contemporary researchers have extracted the chemical components of Curcumae Rhizoma separately for experimental verifcation of its active ingredients in the anti-cancer feld.Numerous studies demonstrated that curcumol,germacrone,β-elemene,and curcumin in Curcumae Rhizoma extracts have signifcant governing effects in anti-cancer activities.Pharmacological studies have shown that Curcumae Rhizoma suppresses cancer cell proliferation,invasion,and migration,triggering apoptosis and regulating cellular autophagy to achieve anticancer effects.Here,we summarized the research progress of Curcumae Rhizoma on anti-cancer effects from 2013 to 2022,aiming to explore the deeper molecular mechanisms of Curcumae Rhizoma's active components in cancer treatment.展开更多
基金supported by grants from the National Major Project of the People's Republic of China (Nos. 2011ZX08002-004 and 2011ZX08010-004)the Wuhan Science and Technology Project (No. 201260523185)the International Scientific and Technological Cooperation Project of Ministry of Science and Technology of China (No. 2009DFB20290)
文摘Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-induced apoptosis in HepG2 cells. HepG2 cells were incubated with germacrone for 24 h, the protein expression of p-STAT3, STAT3, p-JAK2 and JAK2 was detected by Westem Blotting, and RT-PCR was used to determine the expression of STAT3, p53, Bcl-2 and Bax at transcriptional levels. Besides that, HepG2 cells were pre-treated with AG490 or IL-6 for 2 h, and then incubated with ger- macrone for 24 h. The expression ofp-JAK2, JAK2, p-STAT3, STAT3, p53, Bax and Bcl-2 was detected by Western blotting. The activity of HepG2 cells was tested by MTT assay. The apoptosis of HepG2 cells and levels of reactive oxygen species （ROS） were flow cytometrically measured. The results showed that germacrone exposure decreased p-STAT3 and p-JAK2 and regulated expression of p53 and Bcl-2 family members at the same time. Moreover, IL-6 enhanced the activation of the JAK2/STAT3 signalling pathway and therefore attenuated the germacrone-induced apoptosis. Suppression of JAK2/STAT3 signalling pathway by AG490, an inhibitor of JAK2, resulted in apoptosis and an increase in ROS in response to germacrone exposure. We therefore conclude that germacrone induces apoptosis through the JAK2/STAT3 signalling pathway.
文摘Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detectionwas 100 ng·mL^(-1) for germacrone in plasma and the linear range was 0.1004-15.06 μg·mL^(-1) inplasma. The RSD of intra-day and inter-day assay was 1.87% - 4.29% and 1.29% -5.15%, respectively.The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show anyinterference in the analysis. Conclusion The method is accurate and convenient, and suitable forpharmacokinetic studies of germacrone in rats.
基金supported by the Guangxi Science and Technology Major Project(No.GUIKE AA22096029 and No.AA23023035)Macao Young Scholars Program(No.AM2022022)。
文摘Curcumae Rhizoma,derived from the rhizome of Curcuma phaeocaulis,Curcuma kwangsiensis and Curcuma wenyujin,was called Ezhu in China.In the past,Curcumae Rhizoma extracts were obtained through water decoction or alternative methods,which showed signifcant anti-cancer effects.However,the mixed extracts contain various compound components of Curcumae Rhizoma,leading to an ambiguous mechanism of action for Curcumae Rhizoma extracts anti-cancer.Contemporary researchers have extracted the chemical components of Curcumae Rhizoma separately for experimental verifcation of its active ingredients in the anti-cancer feld.Numerous studies demonstrated that curcumol,germacrone,β-elemene,and curcumin in Curcumae Rhizoma extracts have signifcant governing effects in anti-cancer activities.Pharmacological studies have shown that Curcumae Rhizoma suppresses cancer cell proliferation,invasion,and migration,triggering apoptosis and regulating cellular autophagy to achieve anticancer effects.Here,we summarized the research progress of Curcumae Rhizoma on anti-cancer effects from 2013 to 2022,aiming to explore the deeper molecular mechanisms of Curcumae Rhizoma's active components in cancer treatment.
