North China Plain(NCP) is the primary winter wheat production region in China, characterized by smallholder farming systems. Whereas the winter wheat average yield of smallholder farmers is currently low, the yield po...North China Plain(NCP) is the primary winter wheat production region in China, characterized by smallholder farming systems. Whereas the winter wheat average yield of smallholder farmers is currently low, the yield potential and limiting factors driving the current yield gap remain unclear. Therefore, increasing the wheat yield in NCP is essential for the national food security. This study monitored wheat yield, management practices and soil nutrient data in 132 farmers’ fields of Xushui County, Baoding City, Hebei Province during 2014–2016. These data were analyzed using variance and path analysis to determine the yield gap and the contribution of yield components(i.e., spikes per hectare, grain number per spike and 1 000-grain weight) to wheat yield. Then, the limiting factors of yield components and the optimizing strategies were identified by a boundary line approach. The results showed that the attainable potential yield for winter wheat was 10 514 kg ha^–1. The yield gaps varied strongly between three yield groups(i.e., high, middle and low), which were divided by yield level and contained 44 farmers in each group, and amounted to 2 493, 1 636 and 814 kg ha^–1, respectively. For the three yield components, only spikes per hectare was significantly different(P<0.01) among the three yield groups. For all 132 farmers’ fields, correlation between yield and spikes per hectare(r=0.51, P<0.01), was significantly positive, while correlations with grain number per spike(r=–0.16) and 1 000-grain weight(r=–0.10) were not significant. The path analysis also showed that the spikes per hectare of winter wheat were the most important component to the wheat yield. Boundary line analysis showed that seeding date was the most limiting factor of spikes per hectare with the highest contribution rate(26.7%), followed by basal N input(22.1%) and seeding rate(14.5%), which indicated that management factors in the seeding step were the most important for affecting spikes per hectare. For desired spikes per hectare(>6.598×10^6 ha^–1),the seeding rate should range from 210–300 kg ha^–1, seeding date should range from 3th to 8th October, and basal N input should range from 90–180 kg ha^–1. Compared to these reasonable ranges of management measures, most of the farmers’ practices were not suitable, and both lower and higher levels of management existed. It is concluded that the strategies for optimizing yield components could be achieved by improving wheat seeding quality and optimizing farmers’ nutrient management practices in the NCP.展开更多
Based on the analysis of the grain supply and demand gap’s current situation in China, this paper establishes an indicator system for the influence factors of grain supply and demand gap. Then this paper calculates t...Based on the analysis of the grain supply and demand gap’s current situation in China, this paper establishes an indicator system for the influence factors of grain supply and demand gap. Then this paper calculates the correlation degree between the main grain varieties’ supply and demand gap and its influence factors. The results show that sown area and unit yield have the greatest impact on wheat supply and demand gap;per capita disposable income and unit yield have the greatest impact on corn supply and demand gap;per capita disposable income and agricultural mechanization level have the greatest impact on the supply and demand gap of soybean and rice. From the analysis results, we can obtain the difference between the factors affecting the grain supply and demand gap, and provide a certain theoretical basis and new ideas for the balance of grain supply and demand in China.展开更多
Taking the relevant data of 27 provinces in China during 2013 and 2017 as samples,this paper firstly measured the agricultural total factor productivity( TFP) using Malmquist index method. Then,it built the panel data...Taking the relevant data of 27 provinces in China during 2013 and 2017 as samples,this paper firstly measured the agricultural total factor productivity( TFP) using Malmquist index method. Then,it built the panel data model,and empirically tested the impacts of agricultural TFP on the income gap between urban and rural residents. The results show that the improvement in agricultural TFP can promote the narrowing of the income gap between urban and rural residents,and the factors such as urbanization level and industrial structure also have significant impacts on the income gap between urban and rural residents. On the basis of these,it came up with recommendations,including increasing agricultural human capital investment and establishing agricultural production research institutions.展开更多
目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr ow...目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr owt h f act or,NGF)组和生理盐水(SAL)组,各组又分为1周、2周、4周和8周组。切取吻合近端和远端神经纵切片标本,进行GAP-43的免疫组织化学反应,并结合计算机图像分析进行反应强度的半定量分析。结果:NGF组GAP-43表达显著增高。结论:应用NGF可以促进端侧吻合后神经侧支发芽。展开更多
OBJECTIVE To investigate the role of connexin proteins(Cx),which form gap junctions(GJ),in progression and chemotherapeutic sensitivity of cervical cancer(CaC x).METHODS We analyze the expression of Cx26,Cx30,Cx32 and...OBJECTIVE To investigate the role of connexin proteins(Cx),which form gap junctions(GJ),in progression and chemotherapeutic sensitivity of cervical cancer(CaC x).METHODS We analyze the expression of Cx26,Cx30,Cx32 and Cx43 in human specimens consisting of:Normal cervix(n=78),CaCx FIGO stageⅠ(n=148),CaCx FIGO stageⅡ(n=165).In CaCx cell lines,Hela-Cx32(induced expression by doxycycline),C-33A(endogenously express Cx32)and si Ha(transiently transfected plasmid with Cx32),we detected the role of Cx32 against tostreptonigrin/cisplatin-induced apopotosisin presence or absence of functional GJ through using GJ inhibitors or low density cultural.Furtherly,we observed the relativity of Cx32 and EGFR expression in human specimens.Also,we detected the role of EGFR signaling pathway in the process of Cx32 anti-apoptosis through suppressed EGFR expression by inhibitors or si RNA sequences in cell lines.RESULTS We firstly demonstrated the expression of Cx32 was highly upregulated and accumulated in cytoplasm in the CaCx specimens,and the degree of upregulation correlated with advanced FIGO stages.Thus,in three human cervical cell lines,Cx32 was shown to suppress apoptosis when GJ formation is inhibited.No matter in cases of CaCx or cell lines,Cx32 expression was highly correlated with expression of EGFR and the EGFR pathway is an essential component of the Cx32-induced anti-apoptotic effect.CONCLUSION Cx32,traditionally tumor suppressive protein,was shown to be tumor protective against chemotherapy through EGFR pathway in a GJ-independent way.展开更多
Many medication errors could be avoided if administration was more closely linked with structured monitoring. The contributory factors to administration errors occurred in one recent year were reviewed and identified ...Many medication errors could be avoided if administration was more closely linked with structured monitoring. The contributory factors to administration errors occurred in one recent year were reviewed and identified contributory factors to errors as six domains of administration principles according to the report from the Quality and Safety website. The current measures including guidelines, policy, and practices to prevent the administration errors identified in the previous step were searched from the United Christian Hospital (UCH) homepage. Meanwhile, the international measures suggested in literature were identified to address the administration errors identified. 41 cases were identified as medication errors related to administration error events, with total twenty contributory factors identified according to the incident report which identified five contributory factors as common causes. Measures to prevent interruption of medication round and measures to improve individual knowledge and skills, and personal responsebility were suggested to fill the gaps. The medication administration errors should be avoided through both education reinforcing programme and preventive interventions of distraction or interruption to the procedure after comparing the existing measures to the suggested measures from literature. This study was so important to improve the current measures to prevent medication administration errors.展开更多
Objective：To investigate the effect of the gap junction blocker 1-heptanol on the in vitro chondrogenic differentiation of mouse bone marrow mesenchymal stem cells（MSCs） following induction by GDF-5. Methods：MSCs ...Objective：To investigate the effect of the gap junction blocker 1-heptanol on the in vitro chondrogenic differentiation of mouse bone marrow mesenchymal stem cells（MSCs） following induction by GDF-5. Methods：MSCs were isolated from mouse bone marrow and cultured in vitro. After 3 passages cells were induced to undergo chondrogenic differentiation with recombinant human GDF-5（100 ng/ml）, with or without 1-heptanol（2.5 la mol/L）. The effect of 1-heptanol on MSCs proliferation was investigated using the MTT assay. Type II collagen mRNA and protein were examined by RT-PCR and immunocytochemistry respectively, and the sulfate glycosaminoglycan was assessed by Alcian blue dye staining. Connexin43（Cx43） protein was examined by western blotting. Results：GDF-5 induced proliferation and chondrogenic differentiation of MSCs. While 1-heptanol treatment had no effect on this proliferation, it inhibited the expression of both type II collagen mRNA and protein. The Alcian blue staining revealed that 1-heptanol also inhibited the deposition of the typical cartilage extracellular matrix promoted by recombinant GDF-5. Western blotting demonstrated that 1-heptanol had no effect on the expression of Cx43. Conclusion：These results suggest that mouse bone marrow MSCs can be differentiated into a chondrogenic phenotype by GDF- 5 administration in vitro. While the gap junction blocker, 1-heptanol, did not reduce gap junction Cx43, these intercellular communication pathways clearly played an important functional role in GDF-5-induced cartilage differentiation.展开更多
基金supported by the National Basic Research Program of China (2015CB150405)the Special Fund for Agro-scientific Research in the Public Interest, China (201103003)
文摘North China Plain(NCP) is the primary winter wheat production region in China, characterized by smallholder farming systems. Whereas the winter wheat average yield of smallholder farmers is currently low, the yield potential and limiting factors driving the current yield gap remain unclear. Therefore, increasing the wheat yield in NCP is essential for the national food security. This study monitored wheat yield, management practices and soil nutrient data in 132 farmers’ fields of Xushui County, Baoding City, Hebei Province during 2014–2016. These data were analyzed using variance and path analysis to determine the yield gap and the contribution of yield components(i.e., spikes per hectare, grain number per spike and 1 000-grain weight) to wheat yield. Then, the limiting factors of yield components and the optimizing strategies were identified by a boundary line approach. The results showed that the attainable potential yield for winter wheat was 10 514 kg ha^–1. The yield gaps varied strongly between three yield groups(i.e., high, middle and low), which were divided by yield level and contained 44 farmers in each group, and amounted to 2 493, 1 636 and 814 kg ha^–1, respectively. For the three yield components, only spikes per hectare was significantly different(P<0.01) among the three yield groups. For all 132 farmers’ fields, correlation between yield and spikes per hectare(r=0.51, P<0.01), was significantly positive, while correlations with grain number per spike(r=–0.16) and 1 000-grain weight(r=–0.10) were not significant. The path analysis also showed that the spikes per hectare of winter wheat were the most important component to the wheat yield. Boundary line analysis showed that seeding date was the most limiting factor of spikes per hectare with the highest contribution rate(26.7%), followed by basal N input(22.1%) and seeding rate(14.5%), which indicated that management factors in the seeding step were the most important for affecting spikes per hectare. For desired spikes per hectare(>6.598×10^6 ha^–1),the seeding rate should range from 210–300 kg ha^–1, seeding date should range from 3th to 8th October, and basal N input should range from 90–180 kg ha^–1. Compared to these reasonable ranges of management measures, most of the farmers’ practices were not suitable, and both lower and higher levels of management existed. It is concluded that the strategies for optimizing yield components could be achieved by improving wheat seeding quality and optimizing farmers’ nutrient management practices in the NCP.
文摘Based on the analysis of the grain supply and demand gap’s current situation in China, this paper establishes an indicator system for the influence factors of grain supply and demand gap. Then this paper calculates the correlation degree between the main grain varieties’ supply and demand gap and its influence factors. The results show that sown area and unit yield have the greatest impact on wheat supply and demand gap;per capita disposable income and unit yield have the greatest impact on corn supply and demand gap;per capita disposable income and agricultural mechanization level have the greatest impact on the supply and demand gap of soybean and rice. From the analysis results, we can obtain the difference between the factors affecting the grain supply and demand gap, and provide a certain theoretical basis and new ideas for the balance of grain supply and demand in China.
文摘Taking the relevant data of 27 provinces in China during 2013 and 2017 as samples,this paper firstly measured the agricultural total factor productivity( TFP) using Malmquist index method. Then,it built the panel data model,and empirically tested the impacts of agricultural TFP on the income gap between urban and rural residents. The results show that the improvement in agricultural TFP can promote the narrowing of the income gap between urban and rural residents,and the factors such as urbanization level and industrial structure also have significant impacts on the income gap between urban and rural residents. On the basis of these,it came up with recommendations,including increasing agricultural human capital investment and establishing agricultural production research institutions.
文摘目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr owt h f act or,NGF)组和生理盐水(SAL)组,各组又分为1周、2周、4周和8周组。切取吻合近端和远端神经纵切片标本,进行GAP-43的免疫组织化学反应,并结合计算机图像分析进行反应强度的半定量分析。结果:NGF组GAP-43表达显著增高。结论:应用NGF可以促进端侧吻合后神经侧支发芽。
基金supported by National Nature Science Foundation of China(U1303221)National Natural Science Foundation of China(81373439,81473234)Construction of Technique Plate for Evaluation of the Pharmacodynamics of New Drugs in Xinjiang from the Department of Science and Technology of Xinjiang Province(201233150)
文摘OBJECTIVE To investigate the role of connexin proteins(Cx),which form gap junctions(GJ),in progression and chemotherapeutic sensitivity of cervical cancer(CaC x).METHODS We analyze the expression of Cx26,Cx30,Cx32 and Cx43 in human specimens consisting of:Normal cervix(n=78),CaCx FIGO stageⅠ(n=148),CaCx FIGO stageⅡ(n=165).In CaCx cell lines,Hela-Cx32(induced expression by doxycycline),C-33A(endogenously express Cx32)and si Ha(transiently transfected plasmid with Cx32),we detected the role of Cx32 against tostreptonigrin/cisplatin-induced apopotosisin presence or absence of functional GJ through using GJ inhibitors or low density cultural.Furtherly,we observed the relativity of Cx32 and EGFR expression in human specimens.Also,we detected the role of EGFR signaling pathway in the process of Cx32 anti-apoptosis through suppressed EGFR expression by inhibitors or si RNA sequences in cell lines.RESULTS We firstly demonstrated the expression of Cx32 was highly upregulated and accumulated in cytoplasm in the CaCx specimens,and the degree of upregulation correlated with advanced FIGO stages.Thus,in three human cervical cell lines,Cx32 was shown to suppress apoptosis when GJ formation is inhibited.No matter in cases of CaCx or cell lines,Cx32 expression was highly correlated with expression of EGFR and the EGFR pathway is an essential component of the Cx32-induced anti-apoptotic effect.CONCLUSION Cx32,traditionally tumor suppressive protein,was shown to be tumor protective against chemotherapy through EGFR pathway in a GJ-independent way.
文摘Many medication errors could be avoided if administration was more closely linked with structured monitoring. The contributory factors to administration errors occurred in one recent year were reviewed and identified contributory factors to errors as six domains of administration principles according to the report from the Quality and Safety website. The current measures including guidelines, policy, and practices to prevent the administration errors identified in the previous step were searched from the United Christian Hospital (UCH) homepage. Meanwhile, the international measures suggested in literature were identified to address the administration errors identified. 41 cases were identified as medication errors related to administration error events, with total twenty contributory factors identified according to the incident report which identified five contributory factors as common causes. Measures to prevent interruption of medication round and measures to improve individual knowledge and skills, and personal responsebility were suggested to fill the gaps. The medication administration errors should be avoided through both education reinforcing programme and preventive interventions of distraction or interruption to the procedure after comparing the existing measures to the suggested measures from literature. This study was so important to improve the current measures to prevent medication administration errors.
基金supported by the National Natural Science Foundation of China(30471753)
文摘Objective：To investigate the effect of the gap junction blocker 1-heptanol on the in vitro chondrogenic differentiation of mouse bone marrow mesenchymal stem cells（MSCs） following induction by GDF-5. Methods：MSCs were isolated from mouse bone marrow and cultured in vitro. After 3 passages cells were induced to undergo chondrogenic differentiation with recombinant human GDF-5（100 ng/ml）, with or without 1-heptanol（2.5 la mol/L）. The effect of 1-heptanol on MSCs proliferation was investigated using the MTT assay. Type II collagen mRNA and protein were examined by RT-PCR and immunocytochemistry respectively, and the sulfate glycosaminoglycan was assessed by Alcian blue dye staining. Connexin43（Cx43） protein was examined by western blotting. Results：GDF-5 induced proliferation and chondrogenic differentiation of MSCs. While 1-heptanol treatment had no effect on this proliferation, it inhibited the expression of both type II collagen mRNA and protein. The Alcian blue staining revealed that 1-heptanol also inhibited the deposition of the typical cartilage extracellular matrix promoted by recombinant GDF-5. Western blotting demonstrated that 1-heptanol had no effect on the expression of Cx43. Conclusion：These results suggest that mouse bone marrow MSCs can be differentiated into a chondrogenic phenotype by GDF- 5 administration in vitro. While the gap junction blocker, 1-heptanol, did not reduce gap junction Cx43, these intercellular communication pathways clearly played an important functional role in GDF-5-induced cartilage differentiation.
