In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology bas...In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients.展开更多
Pse-in-One 2.0 is a package of web-servers evolved from Pse-in-One (Liu, B., Liu, F., Wang, X., Chen, J. Fang, L. & Chou, K.C. Nucleic Acids Research, 2015, 43:W65-W71). In order to make it more flexible and compr...Pse-in-One 2.0 is a package of web-servers evolved from Pse-in-One (Liu, B., Liu, F., Wang, X., Chen, J. Fang, L. & Chou, K.C. Nucleic Acids Research, 2015, 43:W65-W71). In order to make it more flexible and comprehensive as suggested by many users, the updated package has incorporated 23 new pseudo component modes as well as a series of new feature analysis approaches. It is available at http://bioinformatics.hitsz.edu.cn/Pse-in-One2.0/. Moreover, to maximize the convenience of users, provided is also the stand-alone version called “Pse-in-One-Analysis”, by which users can significantly speed up the analysis of massive sequences.展开更多
Component sequence preservation is an intrinsic requirement in typical engineering applications, such as deployable chain-likestructures, 3D printing structures with contour-parallel toolpaths, additive manufacturing ...Component sequence preservation is an intrinsic requirement in typical engineering applications, such as deployable chain-likestructures, 3D printing structures with contour-parallel toolpaths, additive manufacturing of continuous fibre-reinforcedpolymer structures, customized stents, and soft robotics parts. This study presents a feature-driven method that preservescomponent sequences accounting for engineering requirements. The chain-of-bars design variables setting scheme is developedto realize the sequential component’s layout, which sets the current bar’s end point as the next bar’s start point. The total lengthof the printing path is constrained to reduce the consumption of material accurately. Also, the angle between adjacent bars isconstrained to avoid sharp angles at the turning point of the 3D printing path. Next, the sensitivity analysis considering theinter-dependence of substructures is performed. Several numerical examples are given to demonstrate the validity and merits ofthe proposed method in designing structures preserving component sequences.展开更多
Disassembly sequence planning is an important step of mechanical maintenance. This article presents an integrated study about the generation and optimizing algorithm of the disassembly sequence. Mechanical products ar...Disassembly sequence planning is an important step of mechanical maintenance. This article presents an integrated study about the generation and optimizing algorithm of the disassembly sequence. Mechanical products are divided into two categories of components and connectors. The article uses component-joint graph to represent assembly constraints, including the incidence constraints are represented by incidence matrix and the interference constraints are represented by interference constraints. The inspiring factor and pheromone matrix are calculated according to assembly constraints. Then the ant generates its own disassembly sequences one by one and updates the inspiring factor and pheromone matrix. After all iterations, the best disassembly sequence planning of components and connectors are given. Finally, an application instance of the disassembly sequence of the jack is presented to illustrate the validity of this method.展开更多
AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of ...AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of DLC on the intestinal microbiota in T2DM rats was analyzed via 16S rDNA sequencing.Targeted metabolomics was conducted to evaluate the impact of DLC on the levels of nine short-chain fatty acids(SCFAs).Untargeted metabolomics examined DLC-induced alterations in fecal metabolites and associated metabolic pathways.Additionally,Spearman’s correlation analysis assessed gut microbiota and fecal metabolite relationships.RESULTS DLC significantly attenuated pathological weight loss,reduced fasting blood glucose levels,restored blood sugar homeostasis,and ameliorated dyslipidemia in T2DM rats.The 16S rDNA sequencing revealed that DLC enhanced microbial diversity and reversed intestinal dysbiosis.Targeted metabolomics indicated decreased acetic acid and propionic acid levels and increased butyric acid,isobutyric acid,and 2-methylbutyric acid levels after DLC treatment.Untargeted metabolomics revealed 57 metabolites with altered expression associated with amino acid,carbohydrate,purine,and biotin pathways.The Spearman analysis demonstrated significant links between specific gut microbiota taxa and fecal metabolites.CONCLUSION DLC may exert hypoglycemic effects by modulating intestinal flora genera,SCFA levels,and fecal metabolites.展开更多
BACKGROUND Despite an expanding number of studies on intraductal papillary neoplasm of the bile duct(IPNB),distant metastasis remains unexplained especially in cases of carcinoma in situ.In the present study,we report...BACKGROUND Despite an expanding number of studies on intraductal papillary neoplasm of the bile duct(IPNB),distant metastasis remains unexplained especially in cases of carcinoma in situ.In the present study,we report a rare and interesting case of IPNB without invasive components that later metastasized to lungs and brain.CASE SUMMARY A 69-year-old male was referred to our hospital due to suspected cholangiocarcinoma.Laboratory tests on admission reported a mild elevation of alkaline phosphatase,γ-glutamyl transpeptidase,and total bilirubin in serum.Endoscopic retrograde cholangiography revealed a filling defect in the common bile duct(CBD)extending to the left hepatic duct.Peroral cholangioscopy delineated a tumor in the CBD that had a papillary pattern.Multidetector computed tomography and magnetic resonance cholangiopancreatography detected partial blockage ot interlude in the CBD leading to cholestasis without evidence of metastasis.Therefore,a diagnosis of IPNB cT1N0M0 was established.Left hepatectomy with bile duct reconstruction was performed.Pathological examination confirmed an intraepithelial neoplasia pattern without an invasive component and an R0 resection achievement.The patient was monitored carefully by regular examinations.However,at 32 mo after the operation,a 26 mm tumor in the lungs and a 12 mm lesion in the brain were detected following a suspicious elevated CA 19-9 level.Video-assisted thoracoscopic surgery of left upper lobectomy and stereotactic radiotherapy are indicated.In addition to histopathological results,a genomic profiling analysis using whole exome sequencing subsequently confirmed lung metastasis originating from bile duct cancer.CONCLUSION This case highlights the important role of genomic profiling analysis using whole exome sequencing in identifying the origin of metastasis in patients with IPNB.展开更多
To avoid the complexity and inefficiency for specific applications of the current software architecture, a novel approach using partial evaluation is proposed to improve the running performance of components. The gene...To avoid the complexity and inefficiency for specific applications of the current software architecture, a novel approach using partial evaluation is proposed to improve the running performance of components. The generic program was specialized into domain-specific realization for the known knowledge and environments. The syntax and semantic(adj.) were analyzed based on byte code instruction sequences, and partial evaluation rules depicted how to perform the specialization. The partial evaluation for object-oriented programs was implemented. The experimental results show that partial evaluation is effective to speed up the running efficiency. The more generality and scalability can be obtained by the integration of partial evaluation with the favorable design mechanisms and compiler optimization technology.展开更多
This paper considers a material constrained component scheduling problem during the high speed surface mount manufacturing stage in printed circuit board (PCB) assembly, where each piece of board contains an even numb...This paper considers a material constrained component scheduling problem during the high speed surface mount manufacturing stage in printed circuit board (PCB) assembly, where each piece of board contains an even number of identical PCBs. To accomplish the production, material requirements must be predetermined and incorporated as restraints into the scheduling problem, which has the objective of minimizing production completion time (makespan). A solution procedure is developed based on the following strategies: 1) Each machine is responsible for the same PCBs of each piece, 2) Components of the same types may use one or more feeder locations, 3) Component types are clustered based on their suitable placement speeds, 4) A heuristic using a bottom-up approach is applied to determine the component placement sequence and the feeder location assignment for all machines. Velocity estimate functions of the turret, XY table, and feeder carriage were derived based on empirical data. An experiment using Fuji CP732E machines was conducted on two real life instances. Experimental results indicate that our method performs 32.96% and 10.60% better than the Fuji-CP software for the two instances, in terms of the makespan per piece of board.展开更多
The use of dynamic programming(DP)algorithms to learn Bayesian network structures is limited by their high space complexity and difficulty in learning the structure of large-scale networks.Therefore,this study propose...The use of dynamic programming(DP)algorithms to learn Bayesian network structures is limited by their high space complexity and difficulty in learning the structure of large-scale networks.Therefore,this study proposes a DP algorithm based on node block sequence constraints.The proposed algorithm constrains the traversal process of the parent graph by using the M-sequence matrix to considerably reduce the time consumption and space complexity by pruning the traversal process of the order graph using the node block sequence.Experimental results show that compared with existing DP algorithms,the proposed algorithm can obtain learning results more efficiently with less than 1%loss of accuracy,and can be used for learning larger-scale networks.展开更多
Fast and accurate acquisition of positive sequence components of unbalanced grid voltage is an essential requirement to ensure the safety operation of the grid-connected inverter.To improve the extraction speed of pos...Fast and accurate acquisition of positive sequence components of unbalanced grid voltage is an essential requirement to ensure the safety operation of the grid-connected inverter.To improve the extraction speed of positive sequence components of unbalanced voltage,this study proposes a sampling period delay filter(SPDF)to quickly separate positive and negative sequence components by delaying two sampling periods of grid voltage in dq frame.With the SPDF method,only one coordinate transformation is required and the computational burden can be reduced apparently.Then,the noise immunity performance of the proposed SPDF algorithm is investigated;and the corresponding solution,operation period delay filter(OPDF),can guarantee the desired fast response performance under the premise of limiting the amplified noise within the acceptable range.Finally,the feasibility and priority of the above two algorithms have been verified by the simulation and experimental results.展开更多
BACKGROUND Gastric cancer(GC), a multifactorial disease, is caused by pathogens, such as Helicobacter pylori(H. pylori) and Epstein-Barr virus(EBV), and genetic components.AIM To investigate microbiomes and host genom...BACKGROUND Gastric cancer(GC), a multifactorial disease, is caused by pathogens, such as Helicobacter pylori(H. pylori) and Epstein-Barr virus(EBV), and genetic components.AIM To investigate microbiomes and host genome instability by cost-effective,low-coverage wholegenome sequencing,as biomarkers for GC subtyping.METHODS Samples from 40 GC patients were collected from Taizhou Hospital,Zhejiang Province,affiliated with Wenzhou Medical University.DNA from the samples was subjected to low-coverage wholegenome sequencing with a median genome coverage of 1.86×(range:1.03×to 3.17×) by Illumina×10,followed by copy number analyses using a customized bioinformatics workflow ultrasensitive chromosomal aneuploidy detector.RESULTS Of the 40 GC samples,20 (50%) were found to be enriched with microbiomes.EBV DNA was detected in 5 GC patients (12.5%).H.pylori DNA was found in 15 (37.5%) patients.The other 20(50%) patients were found to have relatively higher genomic instability.Copy number amplifications of the oncogenes,ERBB2 and KRAS,were found in 9 (22.5%) and 7 (17.5%) of the GC samples,respectively.EBV enrichment was found to be associated with tumors in the gastric cardia and fundus.H.pylori enrichment was found to be associated with tumors in the pylorus and antrum.Tumors with elevated genomic instability showed no localization and could be observed in any location.Additionally,H.pylori-enriched GC was found to be associated with the Borrmann type Ⅱ/Ⅲ and gastritis history.EBV-enriched GC was not associated with gastritis.No statistically significant correlation was observed between genomic instability and gastritis.Furthermore,these three different molecular subtypes showed distinct survival outcomes (P=0.019).EBV-positive tumors had the best prognosis,whereas patients with high genomic instability (CIN+) showed the worst survival.Patients with H.pylori infection showed intermediate prognosis compared with the other two subtypes.CONCLUSION Thus,using low-coverage whole-genome sequencing,GC can be classified into three categories based on disease etiology;this classification may prove useful for GC diagnosis and precision medicine.展开更多
The estimation of sequence or symmetrical components and frequency in three-phase unbalanced power system is of great importance for protection and relay.This paper proposes a new H∞filter based on sparse model to tr...The estimation of sequence or symmetrical components and frequency in three-phase unbalanced power system is of great importance for protection and relay.This paper proposes a new H∞filter based on sparse model to track the sequence components and the frequency of three-phase unbalanced power systems.The inclusion of sparsity improves the error convergence behavior of estimation model and hence short-duration non-stationary PQ events can easily be tracked in the time domain.The proposed model is developed using l1 norm penalty in the cost function of H∞filter,which is quite suitable for estimation across all the three phases of an unbalanced system.This model uses real state space modeling across three phases to estimate amplitude and phase parameters of sequence components.However,frequency estimation uses complex state space modeling and Clarke transformation generates a complex measurement signal from the unbalanced three-phase voltages.The state vector used for frequency estimation consists of two state variables.The proposed sparse model is tested using distorted three-phase signals from IEEE-1159-PQE database and the data generated from experimental laboratory setup.The analysis of absolute and mean square error is presented to validate the performance of the proposed model.展开更多
基金financially supported by National Key R&D Program(2021YFF0701905)。
文摘In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients.
文摘Pse-in-One 2.0 is a package of web-servers evolved from Pse-in-One (Liu, B., Liu, F., Wang, X., Chen, J. Fang, L. & Chou, K.C. Nucleic Acids Research, 2015, 43:W65-W71). In order to make it more flexible and comprehensive as suggested by many users, the updated package has incorporated 23 new pseudo component modes as well as a series of new feature analysis approaches. It is available at http://bioinformatics.hitsz.edu.cn/Pse-in-One2.0/. Moreover, to maximize the convenience of users, provided is also the stand-alone version called “Pse-in-One-Analysis”, by which users can significantly speed up the analysis of massive sequences.
基金supported by the Chinese Studentship Council(Grant No.201908060224)the Young Talent Fund of Association for Science and Technology in Shaanxi,China(Grant No.20230240)+1 种基金the National Natural Science Foundation of China(Grant No.11972308)Queen Mary University of London with the PhD fee waiver.
文摘Component sequence preservation is an intrinsic requirement in typical engineering applications, such as deployable chain-likestructures, 3D printing structures with contour-parallel toolpaths, additive manufacturing of continuous fibre-reinforcedpolymer structures, customized stents, and soft robotics parts. This study presents a feature-driven method that preservescomponent sequences accounting for engineering requirements. The chain-of-bars design variables setting scheme is developedto realize the sequential component’s layout, which sets the current bar’s end point as the next bar’s start point. The total lengthof the printing path is constrained to reduce the consumption of material accurately. Also, the angle between adjacent bars isconstrained to avoid sharp angles at the turning point of the 3D printing path. Next, the sensitivity analysis considering theinter-dependence of substructures is performed. Several numerical examples are given to demonstrate the validity and merits ofthe proposed method in designing structures preserving component sequences.
文摘Disassembly sequence planning is an important step of mechanical maintenance. This article presents an integrated study about the generation and optimizing algorithm of the disassembly sequence. Mechanical products are divided into two categories of components and connectors. The article uses component-joint graph to represent assembly constraints, including the incidence constraints are represented by incidence matrix and the interference constraints are represented by interference constraints. The inspiring factor and pheromone matrix are calculated according to assembly constraints. Then the ant generates its own disassembly sequences one by one and updates the inspiring factor and pheromone matrix. After all iterations, the best disassembly sequence planning of components and connectors are given. Finally, an application instance of the disassembly sequence of the jack is presented to illustrate the validity of this method.
基金Supported by the National Natural Science Foundation of China,No.82160771NATCM's Project of High-Level Construction of Key TCM Disciplines:Traditional Medicine of Chinese Minority(Zhuang Medicine),No.zyyzdxk-2023165+7 种基金Guangxi One Thousand Young and Middle-Aged College and University Backbones Teachers Cultivation Program,No.[2019]5Guangxi Traditional Chinese Medicine Multidisciplinary Cross Innovation Team Project,No.GZKJ2309Guangxi Key R&D Plan Project,No.AB21196016Guangxi Key Discipline of Traditional Chinese Medicine Zhuang Pharmacy,No.GZXK-Z-20-64The First-Class Subject of Traditional Chinese Medicine(Ethnic Pharmacy)in Guangxi,No.[2018]12Guangxi Science and Technology Base and Talent Special Project,No.AD20238058 and No.AD21238031the Third Batch of Cultivating High-level Talent Teams in the“Qi Huang Project”of the Guangxi University of Chinese Medicine,No.202406and Huang Danian Style Teacher Team From Universities in Guangxi Zhuang Autonomous Region“Traditional Chinese Medicine Inheritance and Innovation Teacher Team”,No.[2023]31.
文摘AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of DLC on the intestinal microbiota in T2DM rats was analyzed via 16S rDNA sequencing.Targeted metabolomics was conducted to evaluate the impact of DLC on the levels of nine short-chain fatty acids(SCFAs).Untargeted metabolomics examined DLC-induced alterations in fecal metabolites and associated metabolic pathways.Additionally,Spearman’s correlation analysis assessed gut microbiota and fecal metabolite relationships.RESULTS DLC significantly attenuated pathological weight loss,reduced fasting blood glucose levels,restored blood sugar homeostasis,and ameliorated dyslipidemia in T2DM rats.The 16S rDNA sequencing revealed that DLC enhanced microbial diversity and reversed intestinal dysbiosis.Targeted metabolomics indicated decreased acetic acid and propionic acid levels and increased butyric acid,isobutyric acid,and 2-methylbutyric acid levels after DLC treatment.Untargeted metabolomics revealed 57 metabolites with altered expression associated with amino acid,carbohydrate,purine,and biotin pathways.The Spearman analysis demonstrated significant links between specific gut microbiota taxa and fecal metabolites.CONCLUSION DLC may exert hypoglycemic effects by modulating intestinal flora genera,SCFA levels,and fecal metabolites.
文摘BACKGROUND Despite an expanding number of studies on intraductal papillary neoplasm of the bile duct(IPNB),distant metastasis remains unexplained especially in cases of carcinoma in situ.In the present study,we report a rare and interesting case of IPNB without invasive components that later metastasized to lungs and brain.CASE SUMMARY A 69-year-old male was referred to our hospital due to suspected cholangiocarcinoma.Laboratory tests on admission reported a mild elevation of alkaline phosphatase,γ-glutamyl transpeptidase,and total bilirubin in serum.Endoscopic retrograde cholangiography revealed a filling defect in the common bile duct(CBD)extending to the left hepatic duct.Peroral cholangioscopy delineated a tumor in the CBD that had a papillary pattern.Multidetector computed tomography and magnetic resonance cholangiopancreatography detected partial blockage ot interlude in the CBD leading to cholestasis without evidence of metastasis.Therefore,a diagnosis of IPNB cT1N0M0 was established.Left hepatectomy with bile duct reconstruction was performed.Pathological examination confirmed an intraepithelial neoplasia pattern without an invasive component and an R0 resection achievement.The patient was monitored carefully by regular examinations.However,at 32 mo after the operation,a 26 mm tumor in the lungs and a 12 mm lesion in the brain were detected following a suspicious elevated CA 19-9 level.Video-assisted thoracoscopic surgery of left upper lobectomy and stereotactic radiotherapy are indicated.In addition to histopathological results,a genomic profiling analysis using whole exome sequencing subsequently confirmed lung metastasis originating from bile duct cancer.CONCLUSION This case highlights the important role of genomic profiling analysis using whole exome sequencing in identifying the origin of metastasis in patients with IPNB.
基金Sponsored by the National High-Tech Research and Development Program of China (Grant No 2001AA113160,2004AA104280,and 2007AA010302)the National Natural Science Foundation of China(Grant No90718004)
文摘To avoid the complexity and inefficiency for specific applications of the current software architecture, a novel approach using partial evaluation is proposed to improve the running performance of components. The generic program was specialized into domain-specific realization for the known knowledge and environments. The syntax and semantic(adj.) were analyzed based on byte code instruction sequences, and partial evaluation rules depicted how to perform the specialization. The partial evaluation for object-oriented programs was implemented. The experimental results show that partial evaluation is effective to speed up the running efficiency. The more generality and scalability can be obtained by the integration of partial evaluation with the favorable design mechanisms and compiler optimization technology.
文摘This paper considers a material constrained component scheduling problem during the high speed surface mount manufacturing stage in printed circuit board (PCB) assembly, where each piece of board contains an even number of identical PCBs. To accomplish the production, material requirements must be predetermined and incorporated as restraints into the scheduling problem, which has the objective of minimizing production completion time (makespan). A solution procedure is developed based on the following strategies: 1) Each machine is responsible for the same PCBs of each piece, 2) Components of the same types may use one or more feeder locations, 3) Component types are clustered based on their suitable placement speeds, 4) A heuristic using a bottom-up approach is applied to determine the component placement sequence and the feeder location assignment for all machines. Velocity estimate functions of the turret, XY table, and feeder carriage were derived based on empirical data. An experiment using Fuji CP732E machines was conducted on two real life instances. Experimental results indicate that our method performs 32.96% and 10.60% better than the Fuji-CP software for the two instances, in terms of the makespan per piece of board.
基金Shaanxi Science Fund for Distinguished Young Scholars,Grant/Award Number:2024JC-JCQN-57Xi’an Science and Technology Plan Project,Grant/Award Number:2023JH-QCYJQ-0086+2 种基金Scientific Research Program Funded by Education Department of Shaanxi Provincial Government,Grant/Award Number:P23JP071Engineering Technology Research Center of Shaanxi Province for Intelligent Testing and Reliability Evaluation of Electronic Equipments,Grant/Award Number:2023-ZC-GCZX-00472022 Shaanxi University Youth Innovation Team Project。
文摘The use of dynamic programming(DP)algorithms to learn Bayesian network structures is limited by their high space complexity and difficulty in learning the structure of large-scale networks.Therefore,this study proposes a DP algorithm based on node block sequence constraints.The proposed algorithm constrains the traversal process of the parent graph by using the M-sequence matrix to considerably reduce the time consumption and space complexity by pruning the traversal process of the order graph using the node block sequence.Experimental results show that compared with existing DP algorithms,the proposed algorithm can obtain learning results more efficiently with less than 1%loss of accuracy,and can be used for learning larger-scale networks.
基金The work was supported by the National Natural Science Foundation of China(51707091).
文摘Fast and accurate acquisition of positive sequence components of unbalanced grid voltage is an essential requirement to ensure the safety operation of the grid-connected inverter.To improve the extraction speed of positive sequence components of unbalanced voltage,this study proposes a sampling period delay filter(SPDF)to quickly separate positive and negative sequence components by delaying two sampling periods of grid voltage in dq frame.With the SPDF method,only one coordinate transformation is required and the computational burden can be reduced apparently.Then,the noise immunity performance of the proposed SPDF algorithm is investigated;and the corresponding solution,operation period delay filter(OPDF),can guarantee the desired fast response performance under the premise of limiting the amplified noise within the acceptable range.Finally,the feasibility and priority of the above two algorithms have been verified by the simulation and experimental results.
基金Supported by Program of Taizhou Science and Technology Grant,No.20ywb29Medical Health Science and Technology Project of Zhejiang Province,No.2021PY083+2 种基金Key Technology Research and Development Program of Zhejiang Province,No.2019C03040Open Project Program of Key Laboratory of Minimally Invasive Techniques & Rapid Rehabilitation of Digestive System Tumor of Zhejiang Province,No.21SZDSYS01 and 21SZDSYS09Major Research Program of Taizhou Enze Medical Center Grant,No.19EZZDA2
文摘BACKGROUND Gastric cancer(GC), a multifactorial disease, is caused by pathogens, such as Helicobacter pylori(H. pylori) and Epstein-Barr virus(EBV), and genetic components.AIM To investigate microbiomes and host genome instability by cost-effective,low-coverage wholegenome sequencing,as biomarkers for GC subtyping.METHODS Samples from 40 GC patients were collected from Taizhou Hospital,Zhejiang Province,affiliated with Wenzhou Medical University.DNA from the samples was subjected to low-coverage wholegenome sequencing with a median genome coverage of 1.86×(range:1.03×to 3.17×) by Illumina×10,followed by copy number analyses using a customized bioinformatics workflow ultrasensitive chromosomal aneuploidy detector.RESULTS Of the 40 GC samples,20 (50%) were found to be enriched with microbiomes.EBV DNA was detected in 5 GC patients (12.5%).H.pylori DNA was found in 15 (37.5%) patients.The other 20(50%) patients were found to have relatively higher genomic instability.Copy number amplifications of the oncogenes,ERBB2 and KRAS,were found in 9 (22.5%) and 7 (17.5%) of the GC samples,respectively.EBV enrichment was found to be associated with tumors in the gastric cardia and fundus.H.pylori enrichment was found to be associated with tumors in the pylorus and antrum.Tumors with elevated genomic instability showed no localization and could be observed in any location.Additionally,H.pylori-enriched GC was found to be associated with the Borrmann type Ⅱ/Ⅲ and gastritis history.EBV-enriched GC was not associated with gastritis.No statistically significant correlation was observed between genomic instability and gastritis.Furthermore,these three different molecular subtypes showed distinct survival outcomes (P=0.019).EBV-positive tumors had the best prognosis,whereas patients with high genomic instability (CIN+) showed the worst survival.Patients with H.pylori infection showed intermediate prognosis compared with the other two subtypes.CONCLUSION Thus,using low-coverage whole-genome sequencing,GC can be classified into three categories based on disease etiology;this classification may prove useful for GC diagnosis and precision medicine.
基金the support of Indian Institute of Information Technology,Bhubaneswar,IndiaVeer Surendra Sai University of Tecnology(Burla),Sambalpur,India,in terms of Laboratory and online Journal facilities to carry out this research work
文摘The estimation of sequence or symmetrical components and frequency in three-phase unbalanced power system is of great importance for protection and relay.This paper proposes a new H∞filter based on sparse model to track the sequence components and the frequency of three-phase unbalanced power systems.The inclusion of sparsity improves the error convergence behavior of estimation model and hence short-duration non-stationary PQ events can easily be tracked in the time domain.The proposed model is developed using l1 norm penalty in the cost function of H∞filter,which is quite suitable for estimation across all the three phases of an unbalanced system.This model uses real state space modeling across three phases to estimate amplitude and phase parameters of sequence components.However,frequency estimation uses complex state space modeling and Clarke transformation generates a complex measurement signal from the unbalanced three-phase voltages.The state vector used for frequency estimation consists of two state variables.The proposed sparse model is tested using distorted three-phase signals from IEEE-1159-PQE database and the data generated from experimental laboratory setup.The analysis of absolute and mean square error is presented to validate the performance of the proposed model.
