BACKGROUND Excessive oxidative stress plays a key role in the development of diabetic complications,including impaired ulcer healing.Previous studies have shown that fish scale ointment can promote wound healing.AIM T...BACKGROUND Excessive oxidative stress plays a key role in the development of diabetic complications,including impaired ulcer healing.Previous studies have shown that fish scale ointment can promote wound healing.AIM To preliminarily investigate the effect of fish scale ointment on wound healing in a diabetic foot ulcer(DFU)rat model by examining its regulation of the nuclear factor E2-related factor 2(Nrf2)pathway and induction of ferroptosis.METHODS Fish scale ointment(collagen product)was prepared from 500 g of silver carp scales.A diabetic rat model was induced by high-fat and high-sugar feeding combined with intraperitoneal streptozotocin injections.For the DFU rat model,ulcer wounds were created by removing dorsal foot hair and cutting the skin to the fascia.The diabetic rats were randomized into five groups:Model,fish scale collagen(FSC),control+liproxstatin-1(Lip-1),model+Lip-1,and FSC+Lip-1.In each group,treatments were administered once daily by topical application and intraperitoneal injection for 14 days.Wound healing was evaluated on days 7 and 14 after treatment.Hematoxylin and eosin staining was used to assess wound injury and capillary formation.Basic fibroblast growth factor(bFGF)and CD31 levels in wound tissue were measured by immunohistochemistry.Additionally,malondialdehyde(MDA),glutathione(GSH),ferroptosis-associated genes,and iron ion concentrations were quantified using assay kits.Protein levels of Nrf2,heme oxygenase-1(HO-1),and glutathione peroxidase 4(GPX4)were determined using Western blotting.RESULTS Compared with the control group,the model group showed slower wound healing,reduced angiogenesis,decreased bFGF and CD31 levels,increased iron ion concentration and MDA levels,reduced GSH levels,and decreased Nrf2,HO-1,and GPX4 protein expression(all P<0.05).The FSC,model+Lip-1,and FSC+Lip-1 groups showed increased wound healing and angiogenesis,elevated bFGF and CD31 expression,lowered iron ion concentration and MDA levels,increased GSH levels,and enhanced Nrf2,HO-1,and GPX4 protein levels compared with the model group(P<0.05).Improvements were more pronounced in the FSC+Lip-1 group compared with the FSC group(P<0.05).CONCLUSION Fish scale ointment promotes angiogenesis and wound healing in DFU rat models by inhibiting ferroptosis,possibly through the activation of the Nrf2 pathway.展开更多
Centrifugal pumps are extensively employed in ocean engineering,such as ship power systems,water transportation,and mineral exploitation.Pressure fluctuation suppression is essential for the operation stability and se...Centrifugal pumps are extensively employed in ocean engineering,such as ship power systems,water transportation,and mineral exploitation.Pressure fluctuation suppression is essential for the operation stability and service life of the centrifugal pump.In this paper,a new method of bionic structure is proposed for the blade surface of a centrifugal pump,which is inspired by the fish scale and comprises a leading edge,a trailing edge,and two symmetrical side edges.This fish scale structure is applied to the blade pressure and suction surfaces,and an impeller with a fish scale structure is constructed.A test rig for a centrifugal pump is developed to determine the pressure fluctuation in the pump with a prototype impeller and fish scale structure impeller.Results reveal that the dominant frequency of pressure fluctuation in volute is the blade passing frequency(f_(bpf))of 193.33 Hz,which is triggered by the interaction between the tongue and the impeller.The bionic structure of the fish scale effectively suppresses the pressure fluctuation amplitude at f_(bpf).From flow rates of 0.6 Q_(d)to 1.2 Q_(d),the average suppressions in pressure fluctuation amplitudes at f_(bpf)are 20.98%,5.85%,19.20%,and 25.77%.展开更多
Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic...Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.展开更多
The microscopic Bouligand-type architectures of fish scales demonstrate a notable efficiency in enhancing the damage tolerance of materials;nevertheless,it is challenging to reproduce in metals.Here bioinspired tungst...The microscopic Bouligand-type architectures of fish scales demonstrate a notable efficiency in enhancing the damage tolerance of materials;nevertheless,it is challenging to reproduce in metals.Here bioinspired tungsten-copper composites with different Bouligand-type architectures mimicking fish scales were fabricated by infiltrating a copper melt into woven contextures of tungsten fibers.These composites exhibit a synergetic enhancement in both strength and ductility at room temperature along with an improved resistance to high-temperature oxidization.The strengths were interpreted by adapting the classical laminate theory to incorporate the characteristics of Bouligand-type architectures.In particular,under load the tungsten fibers can reorient adaptively within the copper matrix by their straightening,stretching,interfacial sliding with the matrix,and the cooperative kinking deformation of fiber grids,representing a successful implementation of the optimizing mechanisms of the Bouligand-type architectures to enhance strength and toughness.This study may serve to promote the development of new high-performance tungsten-copper composites for applications,e.g.,as electrical contacts or heat sinks,and offer a viable approach for constructing bioinspired architectures in metallic materials.展开更多
Fish scale （FS） loaded TiO2 composites were investigated as photocatalysts in degradation of Methyl Orange under solar light irradiation. Composites were prepared through sol-gel method by varying mass ratio of TiO2...Fish scale （FS） loaded TiO2 composites were investigated as photocatalysts in degradation of Methyl Orange under solar light irradiation. Composites were prepared through sol-gel method by varying mass ratio of TiO2/FS at 90：10, 70：30 and 50：50, respectively. The catalysts prepared in this study were characterized by using XRD, SEM, FT-IR and nitrogen sorption. The effects of solar irradiation, mass ratio of TiO//FS composites, irradiation time and catalyst loadings were studied. Synergistic effect was found in TiO2/FS of 90：10 composite which performed higher photocatalytic degradation than synthesized TiO2 under solar light irradiation. However, further increasing fish scale content in the composites reduced the photocatalytic activity drastically. Under solar light irradiation, all the catalysts in this study exhibited photocatalytic activity, except TiO2/FS of 50：50 composite that only acted as a weak biosorbent without performing any photocatalytic property. Photocatalytic degradation increased with increasing catalyst loading and irradiation time but decreased with increased of initial dye concentration.展开更多
To study the current application and development of fish scale collagen at home and abroad, this paper reviews the structural properties, methods of extracting collagen from fish scale and the applications of collagen...To study the current application and development of fish scale collagen at home and abroad, this paper reviews the structural properties, methods of extracting collagen from fish scale and the applications of collagen in biological medicine, food and chemical industries, cosmetics and other fields, discusses the utilization value of fish scale collagen, to provide a theoretical basis for future development and application of fish scale collagen.展开更多
The aims of this study were to prepare natural hydroxyapatite from fish scales(FsHAp)for potential use as a filler in polymer.The FsHAp was prepared from Tilapia fish scales using thermal method.The FsHAp was milled f...The aims of this study were to prepare natural hydroxyapatite from fish scales(FsHAp)for potential use as a filler in polymer.The FsHAp was prepared from Tilapia fish scales using thermal method.The FsHAp was milled for 48 h and dried by spray method.The morphology was characterized using field emission scanning electron microscope(FESEM)which showed irregular shape of FsHAp particles with particle size around 7μm.The analysis of FsHAp was carried out using Fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)to confirm chemical structure of FsHAp.展开更多
The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithi...The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithin, guanine etc. The different extraction methods and the components were analyzed. The research of effective ingredients of fish scales was reviewed as theory basis to further research and comprehensive development.展开更多
Modern technology for developing new items made from renewable resources is becoming more and more popular as a result of rising environmental concern.Recently,contemporary polymer composites have included the hybridi...Modern technology for developing new items made from renewable resources is becoming more and more popular as a result of rising environmental concern.Recently,contemporary polymer composites have included the hybridization of natural fibers with synthetic ones,along with the inclusion of a variety of biowaste filler for developing sustainable goods.In this work,the kenaf/glass hybrid polyester composites are strengthened by the addition of fish scale(FS),which is taken from the fishs outermost layer of skin.Five different stacked-order laminates,such as KKKK,KGKG,GKKG,KGGK,and GGGG,are fabricated by using the hand lay-up method with four different weight concentrations of filler content:0%,5%,10%,and 15%.Mechanical possessions such as tensile,flexural,impact strength and micro-hardness have been evaluated through experimentation in accordance with ASTM standards.The experimental findings revealed that,the tensile strength and micro-hardness value of KGKG laminates with 15wt% of FS filler are found to be maximum of 118.72 MPa and 17.82 HV respectively which are 39.67%and 26.11%greater than that of KGKG laminates without FS filler.However,the flexural and impact strength of same laminates with 10 wt% FS filler exhibited a maximum value of 142.77 MPa and 62.08 kJ/m^(2).In order to corroborate its applicability for structural and building materials in open environment,the dimensional stability of the composite has been studied through moisture absorption test.The influences of FS filler loading on dimensional stability and resistance to moisture absorption capacity of laminates are also investigated.The experimental results reflected that the addition of FS-filler has significantly improved the dimensional stability of the laminates in moist environment by reducing the moisture absorption tendency.To further support the mode of failures,a fractography investigation of fractured surfaces was conducted.展开更多
Acid-soluble collagen (ASC) and pepsin-solubilized collagen (PSC) were prepared from the waste freshwater carp fish scales. The results of SDS-PAGE showed that purified collagens were composed of at least two differen...Acid-soluble collagen (ASC) and pepsin-solubilized collagen (PSC) were prepared from the waste freshwater carp fish scales. The results of SDS-PAGE showed that purified collagens were composed of at least two different chains which were in accordance with the type I collagen with α chain composition of (α1)2α2. Compared with the carp fish ordinary muscle type I collagen , porcine dermis type I collagen and other seawater fish collagens, freshwater carp fish scales collagen contained relative high half-cystine (Cys-s), but lower denaturation temperature(Td) than the porcine dermis type I collagen. These collagens had evident absorption at 230 nm by UV-Vis spectra. The spectrum X-ray diffraction showed that the collagen remained single-helix and tri-helix configuration with the minimum values of the repeat spacings (d) of about 4.48 ? and 11.87 ?. Therefore, to make more effective use of limited-resources, carp fish scales can be a potential resource for the extraction of type I collagen or gelatin.展开更多
The calcium-binding activity of tilapia scale protein hydrolysates sequentially hydrolyzed by trypsin, flavor enzyme and pepsin were investigated. The hydrolysates were divided into four fractions using G-15 gel chrom...The calcium-binding activity of tilapia scale protein hydrolysates sequentially hydrolyzed by trypsin, flavor enzyme and pepsin were investigated. The hydrolysates were divided into four fractions using G-15 gel chromatography, and the F3 fraction has the higher calcium-binding activity of 196.3 mg/g. The UV-vis and the Fourier transform infrared spectroscopy (FTIR) demonstrate that the amino nitrogen atoms and the oxygen atoms belonging to the carboxylate groups are the primary binding sites for Ca2+. The X-ray diffraction and scanning electron microscopy (SEM) confirmed the reaction between the peptde and calcium. The results obtained indicated that this fish scale protein hydroly-sates have potential as functional foods for calcium-supplementation.展开更多
Poor wound healing is an important clinical research concern.Over the past few years,there has been an increasing demand for the use of non-toxic and non-hazardous bioactive compounds to improve wound healing.The aim ...Poor wound healing is an important clinical research concern.Over the past few years,there has been an increasing demand for the use of non-toxic and non-hazardous bioactive compounds to improve wound healing.The aim of this study was to evaluate the effect of fish scale collagen peptide(FSCP)in accelerating wound healing in a mouse full-thickness skin injury model under different modes of administration(gavage and topical).The study showed that both FSCP and its different molecular weight fractions significantly promoted the acceleration of wound healing.The shortening of the scab-shedding time,the increase in the rate of granulation tissue,hair follicle,and capillary production,and the acceleration of collagen remodeling and regeneration were the important signs of the shortening of the wound healing cycle.Reducing the level of oxidative stress and inhibiting the inflammatory response provides a good microenvironmental basis for wound healing.FSCP fractions smaller than 1 kDa had the most significant wound healing promotion effect,with wound healing rates of(99.66±0.48)%and(99.63±0.29)%in the gavage-administered group and topical-administered group,respectively.On this basis,peptides with wound-healing potential,such as APDPFR,GTPIQF,and TPIQF,were identified using LC-MS/MS and in silico methods.This study highlights the potential value of FSCP in promoting wound healing process and provides new bioactive substances for clinical treatment.展开更多
Memory disorders are increasingly recognized as a widespread global concern,attracting significant attention and necessitating urgent intervention.In this study,the effects of fish scale collagen peptide-original flav...Memory disorders are increasingly recognized as a widespread global concern,attracting significant attention and necessitating urgent intervention.In this study,the effects of fish scale collagen peptide-original flavor peptide(FSCP-OP)on memory-impaired mice were examined using behavioral experiments,histopathological staining,and assessments of choline function.The mechanisms by which FSCP-OP enhanced memory function were preliminary exploration through high-resolution mass spectrometry,in silico methods,and molecular docking analyses.It was found that FSCP-OP enhanced learning and cognitive abilities,repaired the pathological state of the mouse hippocampus,increased the number of Nissl bodies in the CA1 and CA3 regions,reduced oxidative stress in the hippocampus,significantly elevated Acetylcholine(ACh)levels to(125.36±10.95)μg/mg prot,and markedly decreased Acetylcholinesterase(AChE)activity to(199.91±12.19)U/mg prot(P<0.05).The molecular docking results for the digestion products GPF and GFD from the FSCP-OP group showed multiple interactions at the AChE binding site,with binding energies of-8.67 kcal/mol and-6.41 kcal/mol,indicating stable conformations.These findings suggest that FSCP-OP is effective in ameliorating memory impairment and that GPF and GFD are promising candidate peptides.展开更多
Tendon-bone healing is essential for an effective rotator cuff tendon repair surgery,however,this remains a significant challenge due to the lack of biomaterials with high strength and bioactivity.Inspired by the high...Tendon-bone healing is essential for an effective rotator cuff tendon repair surgery,however,this remains a significant challenge due to the lack of biomaterials with high strength and bioactivity.Inspired by the high-performance exoskeleton of natural organisms,we set out to apply natural fish scale(FS)modified by calcium silicate nanoparticles(CS NPs)as a new biomaterial(CS-FS)to overcome the challenge.Benefit from its“Bouligand”microstructure,such FS-based scaffold maintained excellent tensile strength(125.05 MPa)and toughness(14.16 MJ/m^(3)),which are 1.93 and 2.72 times that of natural tendon respectively,allowing it to well meet the requirements for rotator cuff tendon repair.Additionally,CS-FS showed diverse bioactivities by stimulating the differentiation and phenotypic maintenance of multiple types of cells participated into the composition of tendon-bone junction,(e.g.bone marrow mesenchymal stem cells(BMSCs),chondrocyte,and tendon stem/progenitor cells(TSPCs)).In both rat and rabbit rotator cuff tear(RCT)models,CS-FS played a key role in the tendon-bone interface regeneration and biomechanical function,which may be achieved by activating BMP-2/Smad/Runx2 pathway in BMSCs.Therefore,natural fish scale-based biomaterials are the promising candidate for clinical tendon repair due to their outstanding strength and bioactivity.展开更多
Tissue engineering scaffolds have presented effective value in bone repair.However,the integration of the diverse components,complex structures,multifunction to impart the scaffolds with improved applicability is stil...Tissue engineering scaffolds have presented effective value in bone repair.However,the integration of the diverse components,complex structures,multifunction to impart the scaffolds with improved applicability is still a challenge.Here,we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells(MSCs)to promote bone regeneration.The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin(fGelMA),which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection.Furthermore,the black phosphorus(BP)nanosheets are incorporated into the fGelMA hydrogel network,which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared(NIR)light.The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro.The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs.These results suggest that the fish-derived scaffold,photothermal therapy,MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration.展开更多
Natural fish scales demonstrate outstanding mechanical efficiency owing to their elaborate architectures and thereby may serve as ideal prototypes for the architectural design of man-made materials.Here bioinspired ma...Natural fish scales demonstrate outstanding mechanical efficiency owing to their elaborate architectures and thereby may serve as ideal prototypes for the architectural design of man-made materials.Here bioinspired magnesium composites with fish-scale-like orthogonal plywood and double-Bouligand architectures were developed by pressureless infiltration of a magnesium melt into the woven contextures of continuous titanium fibers.The composites exhibit enhanced strength and work-hardening ability compared to those estimated from a simple mixture of their constituents at ambient to elevated temperatures.In particular,the double-Bouligand architecture can effectively deflect cracking paths,alleviate strain localization,and adaptively reorient titanium fibers within the magnesium matrix during the deformation of the composite,representing a successful implementation of the property-optimizing mechanisms in fish scales.The strength of the composites,specifically the effect of their bioinspired architectures,was interpreted based on the adaptation of classical laminate theory.This study may offer a feasible approach for developing new bioinspired metal-matrix composites with improved performance and provide theoretical guidance for their architectural designs.展开更多
Fish scales are good sources of gelatin,but the wide application of fish scale gelatin(FSG)is highly restricted due to its poor gelling property.In this study,microbial transglutaminase(MTG)was utilized to improve the...Fish scales are good sources of gelatin,but the wide application of fish scale gelatin(FSG)is highly restricted due to its poor gelling property.In this study,microbial transglutaminase(MTG)was utilized to improve the gelling property of FSG from grass carp.The effects of MTG on the gel strength,textural property,rheological property,triple-helix structure,chemical structure,thermal property,and microstructure of FSG were investigated.Then,the in vitro gastric and simulated intestinal digestibility of the cross-linked FSG were further studied.The cross-linking of FSG by MTG at all concentrations increased the gel strength,hardness,chewiness,peak temperature,and sol-gel transition temperatures.The MTG at a low concentration promoted the formation of an ordered triple-helix structure and a compact network in FSG,while the high-concentration MTG had an opposite effect.Besides,the cross-linking of FSG by MTG generally increased the digestion time of FSG and allowed the release of more unique peptides during gastric and intestinal digestion.Peptides with various bioactivities were released after the simulated intestinal digestion of the native and modified FSGs.This study may provide some guidance for the deep utilization of FSG in the food industry and further promote the exploitation of fish scales.展开更多
Biomimetics has an immense potential to drive the next generation of technologies forward by propounding competent solutions from nature.For decades,the nonsmooth topography of most living creatures has been critical ...Biomimetics has an immense potential to drive the next generation of technologies forward by propounding competent solutions from nature.For decades,the nonsmooth topography of most living creatures has been critical to their existence and survival.Contrary to human-made smooth surfaces,when adapted for fluid flow applications,nonsmooth surfaces can enhance the overall aerodynamic performance by reducing the drag force.Recently,the bioinspired scale structure of fish has been identified as a key biomimetic derivative for improving the aerodynamic efficiency in various cross-domain applications.This study investigates the aerodynamics of a fish scale array(FSA)NACA 0021 model at a specific Reynolds number(Re)of approximately 2.46×105 that is meant for laminar flow conditions using Computational Fluid Dynamics(CFD)tools and a subsonic wind tunnel facility.A 3D printed biomimetic FSA film is developed and affixed on the NACA 0021 wing profile for the experimental investigation,followed by flow visualization through the smoke tunnel facility.As proved qualitatively under specific aerodynamic conditions,the creation of velocity streaks has a major role in the drag reduction process.To obtain a clear perspective of flow across the overlapping fish scale structures,the results are focused on the central and overlapping regions of the FSA structure.The experiment has proved the existence of a combined formation of low and high velocity streaks in central and overlapping regions.The FSA 0021 model showed a maximum drag reduction of 9.57%,which was attributed to the streak formation phenomenon observed in the overlapping FSA configuration.展开更多
基金Supported by the National Natural Science Foundation of China,No.82172095Qingdao Municipal Traditional Chinese Medicine Science and Technology Project,No.2022-zyym03.
文摘BACKGROUND Excessive oxidative stress plays a key role in the development of diabetic complications,including impaired ulcer healing.Previous studies have shown that fish scale ointment can promote wound healing.AIM To preliminarily investigate the effect of fish scale ointment on wound healing in a diabetic foot ulcer(DFU)rat model by examining its regulation of the nuclear factor E2-related factor 2(Nrf2)pathway and induction of ferroptosis.METHODS Fish scale ointment(collagen product)was prepared from 500 g of silver carp scales.A diabetic rat model was induced by high-fat and high-sugar feeding combined with intraperitoneal streptozotocin injections.For the DFU rat model,ulcer wounds were created by removing dorsal foot hair and cutting the skin to the fascia.The diabetic rats were randomized into five groups:Model,fish scale collagen(FSC),control+liproxstatin-1(Lip-1),model+Lip-1,and FSC+Lip-1.In each group,treatments were administered once daily by topical application and intraperitoneal injection for 14 days.Wound healing was evaluated on days 7 and 14 after treatment.Hematoxylin and eosin staining was used to assess wound injury and capillary formation.Basic fibroblast growth factor(bFGF)and CD31 levels in wound tissue were measured by immunohistochemistry.Additionally,malondialdehyde(MDA),glutathione(GSH),ferroptosis-associated genes,and iron ion concentrations were quantified using assay kits.Protein levels of Nrf2,heme oxygenase-1(HO-1),and glutathione peroxidase 4(GPX4)were determined using Western blotting.RESULTS Compared with the control group,the model group showed slower wound healing,reduced angiogenesis,decreased bFGF and CD31 levels,increased iron ion concentration and MDA levels,reduced GSH levels,and decreased Nrf2,HO-1,and GPX4 protein expression(all P<0.05).The FSC,model+Lip-1,and FSC+Lip-1 groups showed increased wound healing and angiogenesis,elevated bFGF and CD31 expression,lowered iron ion concentration and MDA levels,increased GSH levels,and enhanced Nrf2,HO-1,and GPX4 protein levels compared with the model group(P<0.05).Improvements were more pronounced in the FSC+Lip-1 group compared with the FSC group(P<0.05).CONCLUSION Fish scale ointment promotes angiogenesis and wound healing in DFU rat models by inhibiting ferroptosis,possibly through the activation of the Nrf2 pathway.
基金supported by the Open Fund of Science and Technology on Thermal Energy and Power Laboratory[TPL2021A02]the State Key Laboratory of Hydroscience and Engineering[sklhse-2023-E-01].
文摘Centrifugal pumps are extensively employed in ocean engineering,such as ship power systems,water transportation,and mineral exploitation.Pressure fluctuation suppression is essential for the operation stability and service life of the centrifugal pump.In this paper,a new method of bionic structure is proposed for the blade surface of a centrifugal pump,which is inspired by the fish scale and comprises a leading edge,a trailing edge,and two symmetrical side edges.This fish scale structure is applied to the blade pressure and suction surfaces,and an impeller with a fish scale structure is constructed.A test rig for a centrifugal pump is developed to determine the pressure fluctuation in the pump with a prototype impeller and fish scale structure impeller.Results reveal that the dominant frequency of pressure fluctuation in volute is the blade passing frequency(f_(bpf))of 193.33 Hz,which is triggered by the interaction between the tongue and the impeller.The bionic structure of the fish scale effectively suppresses the pressure fluctuation amplitude at f_(bpf).From flow rates of 0.6 Q_(d)to 1.2 Q_(d),the average suppressions in pressure fluctuation amplitudes at f_(bpf)are 20.98%,5.85%,19.20%,and 25.77%.
基金supported by the Scientific Research Foundation of Hainan Tropical Ocean University(No.RHDRC202312)the Major Science and Technology Project of Yazhou Bay Innovation Institute of Hainan Tropical Ocean University:Research on Hainan Coastal Marine Ecological Environment and Carbon Sink(No.2022CXYZD002).
文摘Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.
基金the financial support by the National Key R&D Program of China under grant number 2020YFA0710404the National Natural Science Foundation of China under grant number 51871216+5 种基金the KC Wong Education Foundation(GJTD-2020-09)the Liao Ning Revitalization Talents Programthe State Key Laboratory for Modification of Chemical Fibers and Polymer Materials at Donghua Universitythe Opening Project of Jiangsu Province Key Laboratory of High-End Structural Materials under grant number hsm1801the Youth Innovation Promotion Association CASsupport from the Multidisciplinary University Research Initiative to University of California Riverside,funded by the Air Force Office of Scientific Research(AFOSR-FA9550–15–1–0009)and subcontracted to the University of California Berkeley。
文摘The microscopic Bouligand-type architectures of fish scales demonstrate a notable efficiency in enhancing the damage tolerance of materials;nevertheless,it is challenging to reproduce in metals.Here bioinspired tungsten-copper composites with different Bouligand-type architectures mimicking fish scales were fabricated by infiltrating a copper melt into woven contextures of tungsten fibers.These composites exhibit a synergetic enhancement in both strength and ductility at room temperature along with an improved resistance to high-temperature oxidization.The strengths were interpreted by adapting the classical laminate theory to incorporate the characteristics of Bouligand-type architectures.In particular,under load the tungsten fibers can reorient adaptively within the copper matrix by their straightening,stretching,interfacial sliding with the matrix,and the cooperative kinking deformation of fiber grids,representing a successful implementation of the optimizing mechanisms of the Bouligand-type architectures to enhance strength and toughness.This study may serve to promote the development of new high-performance tungsten-copper composites for applications,e.g.,as electrical contacts or heat sinks,and offer a viable approach for constructing bioinspired architectures in metallic materials.
文摘Fish scale （FS） loaded TiO2 composites were investigated as photocatalysts in degradation of Methyl Orange under solar light irradiation. Composites were prepared through sol-gel method by varying mass ratio of TiO2/FS at 90：10, 70：30 and 50：50, respectively. The catalysts prepared in this study were characterized by using XRD, SEM, FT-IR and nitrogen sorption. The effects of solar irradiation, mass ratio of TiO//FS composites, irradiation time and catalyst loadings were studied. Synergistic effect was found in TiO2/FS of 90：10 composite which performed higher photocatalytic degradation than synthesized TiO2 under solar light irradiation. However, further increasing fish scale content in the composites reduced the photocatalytic activity drastically. Under solar light irradiation, all the catalysts in this study exhibited photocatalytic activity, except TiO2/FS of 50：50 composite that only acted as a weak biosorbent without performing any photocatalytic property. Photocatalytic degradation increased with increasing catalyst loading and irradiation time but decreased with increased of initial dye concentration.
基金Supported by Scientific Research Project of Ezhou Polytechnic(2016YBA51)Science and Technology Research Project of Hubei Provincial Department of Education(B2017531)
文摘To study the current application and development of fish scale collagen at home and abroad, this paper reviews the structural properties, methods of extracting collagen from fish scale and the applications of collagen in biological medicine, food and chemical industries, cosmetics and other fields, discusses the utilization value of fish scale collagen, to provide a theoretical basis for future development and application of fish scale collagen.
文摘The aims of this study were to prepare natural hydroxyapatite from fish scales(FsHAp)for potential use as a filler in polymer.The FsHAp was prepared from Tilapia fish scales using thermal method.The FsHAp was milled for 48 h and dried by spray method.The morphology was characterized using field emission scanning electron microscope(FESEM)which showed irregular shape of FsHAp particles with particle size around 7μm.The analysis of FsHAp was carried out using Fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)to confirm chemical structure of FsHAp.
文摘The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithin, guanine etc. The different extraction methods and the components were analyzed. The research of effective ingredients of fish scales was reviewed as theory basis to further research and comprehensive development.
文摘Modern technology for developing new items made from renewable resources is becoming more and more popular as a result of rising environmental concern.Recently,contemporary polymer composites have included the hybridization of natural fibers with synthetic ones,along with the inclusion of a variety of biowaste filler for developing sustainable goods.In this work,the kenaf/glass hybrid polyester composites are strengthened by the addition of fish scale(FS),which is taken from the fishs outermost layer of skin.Five different stacked-order laminates,such as KKKK,KGKG,GKKG,KGGK,and GGGG,are fabricated by using the hand lay-up method with four different weight concentrations of filler content:0%,5%,10%,and 15%.Mechanical possessions such as tensile,flexural,impact strength and micro-hardness have been evaluated through experimentation in accordance with ASTM standards.The experimental findings revealed that,the tensile strength and micro-hardness value of KGKG laminates with 15wt% of FS filler are found to be maximum of 118.72 MPa and 17.82 HV respectively which are 39.67%and 26.11%greater than that of KGKG laminates without FS filler.However,the flexural and impact strength of same laminates with 10 wt% FS filler exhibited a maximum value of 142.77 MPa and 62.08 kJ/m^(2).In order to corroborate its applicability for structural and building materials in open environment,the dimensional stability of the composite has been studied through moisture absorption test.The influences of FS filler loading on dimensional stability and resistance to moisture absorption capacity of laminates are also investigated.The experimental results reflected that the addition of FS-filler has significantly improved the dimensional stability of the laminates in moist environment by reducing the moisture absorption tendency.To further support the mode of failures,a fractography investigation of fractured surfaces was conducted.
文摘Acid-soluble collagen (ASC) and pepsin-solubilized collagen (PSC) were prepared from the waste freshwater carp fish scales. The results of SDS-PAGE showed that purified collagens were composed of at least two different chains which were in accordance with the type I collagen with α chain composition of (α1)2α2. Compared with the carp fish ordinary muscle type I collagen , porcine dermis type I collagen and other seawater fish collagens, freshwater carp fish scales collagen contained relative high half-cystine (Cys-s), but lower denaturation temperature(Td) than the porcine dermis type I collagen. These collagens had evident absorption at 230 nm by UV-Vis spectra. The spectrum X-ray diffraction showed that the collagen remained single-helix and tri-helix configuration with the minimum values of the repeat spacings (d) of about 4.48 ? and 11.87 ?. Therefore, to make more effective use of limited-resources, carp fish scales can be a potential resource for the extraction of type I collagen or gelatin.
文摘The calcium-binding activity of tilapia scale protein hydrolysates sequentially hydrolyzed by trypsin, flavor enzyme and pepsin were investigated. The hydrolysates were divided into four fractions using G-15 gel chromatography, and the F3 fraction has the higher calcium-binding activity of 196.3 mg/g. The UV-vis and the Fourier transform infrared spectroscopy (FTIR) demonstrate that the amino nitrogen atoms and the oxygen atoms belonging to the carboxylate groups are the primary binding sites for Ca2+. The X-ray diffraction and scanning electron microscopy (SEM) confirmed the reaction between the peptde and calcium. The results obtained indicated that this fish scale protein hydroly-sates have potential as functional foods for calcium-supplementation.
基金support provided by the National Key Research and Development Program of China(2023YFF11052).
文摘Poor wound healing is an important clinical research concern.Over the past few years,there has been an increasing demand for the use of non-toxic and non-hazardous bioactive compounds to improve wound healing.The aim of this study was to evaluate the effect of fish scale collagen peptide(FSCP)in accelerating wound healing in a mouse full-thickness skin injury model under different modes of administration(gavage and topical).The study showed that both FSCP and its different molecular weight fractions significantly promoted the acceleration of wound healing.The shortening of the scab-shedding time,the increase in the rate of granulation tissue,hair follicle,and capillary production,and the acceleration of collagen remodeling and regeneration were the important signs of the shortening of the wound healing cycle.Reducing the level of oxidative stress and inhibiting the inflammatory response provides a good microenvironmental basis for wound healing.FSCP fractions smaller than 1 kDa had the most significant wound healing promotion effect,with wound healing rates of(99.66±0.48)%and(99.63±0.29)%in the gavage-administered group and topical-administered group,respectively.On this basis,peptides with wound-healing potential,such as APDPFR,GTPIQF,and TPIQF,were identified using LC-MS/MS and in silico methods.This study highlights the potential value of FSCP in promoting wound healing process and provides new bioactive substances for clinical treatment.
基金the financial support provided by the National Key Research and Development Program of China(2023YFF11052).
文摘Memory disorders are increasingly recognized as a widespread global concern,attracting significant attention and necessitating urgent intervention.In this study,the effects of fish scale collagen peptide-original flavor peptide(FSCP-OP)on memory-impaired mice were examined using behavioral experiments,histopathological staining,and assessments of choline function.The mechanisms by which FSCP-OP enhanced memory function were preliminary exploration through high-resolution mass spectrometry,in silico methods,and molecular docking analyses.It was found that FSCP-OP enhanced learning and cognitive abilities,repaired the pathological state of the mouse hippocampus,increased the number of Nissl bodies in the CA1 and CA3 regions,reduced oxidative stress in the hippocampus,significantly elevated Acetylcholine(ACh)levels to(125.36±10.95)μg/mg prot,and markedly decreased Acetylcholinesterase(AChE)activity to(199.91±12.19)U/mg prot(P<0.05).The molecular docking results for the digestion products GPF and GFD from the FSCP-OP group showed multiple interactions at the AChE binding site,with binding energies of-8.67 kcal/mol and-6.41 kcal/mol,indicating stable conformations.These findings suggest that FSCP-OP is effective in ameliorating memory impairment and that GPF and GFD are promising candidate peptides.
基金supported by the National Basic Research Program of China(grant No.2021YFA0715700)the Natural Science Foundation of China(32130062)+1 种基金Innovation Cross Team of Chinese Academy of Sciences(JCTD-2018-13)Science and Technology Commission of Shanghai Municipality(20442420300,21DZ1205600).
文摘Tendon-bone healing is essential for an effective rotator cuff tendon repair surgery,however,this remains a significant challenge due to the lack of biomaterials with high strength and bioactivity.Inspired by the high-performance exoskeleton of natural organisms,we set out to apply natural fish scale(FS)modified by calcium silicate nanoparticles(CS NPs)as a new biomaterial(CS-FS)to overcome the challenge.Benefit from its“Bouligand”microstructure,such FS-based scaffold maintained excellent tensile strength(125.05 MPa)and toughness(14.16 MJ/m^(3)),which are 1.93 and 2.72 times that of natural tendon respectively,allowing it to well meet the requirements for rotator cuff tendon repair.Additionally,CS-FS showed diverse bioactivities by stimulating the differentiation and phenotypic maintenance of multiple types of cells participated into the composition of tendon-bone junction,(e.g.bone marrow mesenchymal stem cells(BMSCs),chondrocyte,and tendon stem/progenitor cells(TSPCs)).In both rat and rabbit rotator cuff tear(RCT)models,CS-FS played a key role in the tendon-bone interface regeneration and biomechanical function,which may be achieved by activating BMP-2/Smad/Runx2 pathway in BMSCs.Therefore,natural fish scale-based biomaterials are the promising candidate for clinical tendon repair due to their outstanding strength and bioactivity.
基金the National Key Research and Development Project(No.2021YFA1201404)Key Program of National Natural Science Foundation of China(No.81730067)+4 种基金Major Project of National Natural Science Foundation of China(No.81991514)the National Natural Science Foundation of China(No.82101184)Shenzhen Fundamental Research Program(No.JCYJ20210324102809024)Shenzhen PhD Start-up Program(No.RCBS20210609103713045)Jiangsu Provincial Key Medical Center Foundation,Jiangsu Provincial Medical Outstanding Talent Foundation,Jiangsu Provincial Medical Youth Talent Foundation,Jiangsu Provincial Key Medical Talent Foundation,and the Fundamental Research Funds for the Central Universities(Nos.14380493 and 14380494).
文摘Tissue engineering scaffolds have presented effective value in bone repair.However,the integration of the diverse components,complex structures,multifunction to impart the scaffolds with improved applicability is still a challenge.Here,we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells(MSCs)to promote bone regeneration.The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin(fGelMA),which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection.Furthermore,the black phosphorus(BP)nanosheets are incorporated into the fGelMA hydrogel network,which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared(NIR)light.The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro.The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs.These results suggest that the fish-derived scaffold,photothermal therapy,MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration.
基金the financial support by the National Key R&D Program of China under grant number 2020YFA0710404the National Natural Science Foundation of China under grant number 51871216+6 种基金the KC Wong Education Foundation(GJTD-2020-09)the Liao Ning Revitalization Talents Programthe State Key Laboratory for Modification of Chemical Fibers and Polymer Materials at Donghua Universitythe Opening Project of Jiangsu Province Key Laboratory of High-End Structural Materials under grant number hsm1801the Opening Project of National Key Laboratory of Shock Wave and Detonation Physics under grant number 6142A03203002the Youth Innovation Promotion Association CASsupported by the Multi-University Research Initiative under grant number AFOSR-FA9550-151-0009 from the Air Force Office of Scientific Research
文摘Natural fish scales demonstrate outstanding mechanical efficiency owing to their elaborate architectures and thereby may serve as ideal prototypes for the architectural design of man-made materials.Here bioinspired magnesium composites with fish-scale-like orthogonal plywood and double-Bouligand architectures were developed by pressureless infiltration of a magnesium melt into the woven contextures of continuous titanium fibers.The composites exhibit enhanced strength and work-hardening ability compared to those estimated from a simple mixture of their constituents at ambient to elevated temperatures.In particular,the double-Bouligand architecture can effectively deflect cracking paths,alleviate strain localization,and adaptively reorient titanium fibers within the magnesium matrix during the deformation of the composite,representing a successful implementation of the property-optimizing mechanisms in fish scales.The strength of the composites,specifically the effect of their bioinspired architectures,was interpreted based on the adaptation of classical laminate theory.This study may offer a feasible approach for developing new bioinspired metal-matrix composites with improved performance and provide theoretical guidance for their architectural designs.
基金supported by the Natural Science Foundation of Hubei Province(No.2022CFB162)the Huazhong Agricultural University-Agricultural Genomics Institute at Shenzhen Cooperation Fund(NO.SZYJY2021009)the Agriculture Research System of China(NO.CARS-45-28).
文摘Fish scales are good sources of gelatin,but the wide application of fish scale gelatin(FSG)is highly restricted due to its poor gelling property.In this study,microbial transglutaminase(MTG)was utilized to improve the gelling property of FSG from grass carp.The effects of MTG on the gel strength,textural property,rheological property,triple-helix structure,chemical structure,thermal property,and microstructure of FSG were investigated.Then,the in vitro gastric and simulated intestinal digestibility of the cross-linked FSG were further studied.The cross-linking of FSG by MTG at all concentrations increased the gel strength,hardness,chewiness,peak temperature,and sol-gel transition temperatures.The MTG at a low concentration promoted the formation of an ordered triple-helix structure and a compact network in FSG,while the high-concentration MTG had an opposite effect.Besides,the cross-linking of FSG by MTG generally increased the digestion time of FSG and allowed the release of more unique peptides during gastric and intestinal digestion.Peptides with various bioactivities were released after the simulated intestinal digestion of the native and modified FSGs.This study may provide some guidance for the deep utilization of FSG in the food industry and further promote the exploitation of fish scales.
文摘Biomimetics has an immense potential to drive the next generation of technologies forward by propounding competent solutions from nature.For decades,the nonsmooth topography of most living creatures has been critical to their existence and survival.Contrary to human-made smooth surfaces,when adapted for fluid flow applications,nonsmooth surfaces can enhance the overall aerodynamic performance by reducing the drag force.Recently,the bioinspired scale structure of fish has been identified as a key biomimetic derivative for improving the aerodynamic efficiency in various cross-domain applications.This study investigates the aerodynamics of a fish scale array(FSA)NACA 0021 model at a specific Reynolds number(Re)of approximately 2.46×105 that is meant for laminar flow conditions using Computational Fluid Dynamics(CFD)tools and a subsonic wind tunnel facility.A 3D printed biomimetic FSA film is developed and affixed on the NACA 0021 wing profile for the experimental investigation,followed by flow visualization through the smoke tunnel facility.As proved qualitatively under specific aerodynamic conditions,the creation of velocity streaks has a major role in the drag reduction process.To obtain a clear perspective of flow across the overlapping fish scale structures,the results are focused on the central and overlapping regions of the FSA structure.The experiment has proved the existence of a combined formation of low and high velocity streaks in central and overlapping regions.The FSA 0021 model showed a maximum drag reduction of 9.57%,which was attributed to the streak formation phenomenon observed in the overlapping FSA configuration.
