Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hain...Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hainan Province. The results showed that the optimal fertilizing amount of ternary quadratic model simulation was0.374 kg/plant of N, 0.289 kg/plant of P2O5 and 0.891 kg/plant of K2 O. According to the yield trend characteristic, the optimal fertilizing amount of unary quadratic model was 0.400kg/plant of N, 0.214 kg/plant of P2O5 and 0.901kg/plant of K2 O. Thus it can be seen that only partial indices of the optimal fertilizing amount of ternary quadratic model simulation were higher than that of unary quadratic model. Considering the results, the optimal fertilizing amount of Brazil banana was 0.374-0.400kg/plant of N, 0.214-0.289 kg/plant of P2O5 and 0.891-0.901 kg/plant of K2 O.展开更多
To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amo...To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amount and planting density on yield and quality of material.The results showed that Taishan 27 had high yield under fertilizing amount of 225 kg/ha pure nitrogen and planting density of 240 × 104-300× 10~4/ha; the yield was lowest under fertilizing amount of 300 kg/ha pure nitrogen and planting density of 360 × 10~4/ha. The suitable planting density for Taishan 27 was 240 × 104-300 × 10~4/ha,and the fertilizing amount of nitrogen should be based on different soil fertility conditions to avoid water and fertilizer stress and improve yield.展开更多
Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place...Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place as the spermatozoa pass through the female reproductive tract(FRT).Dihydrolipoamide dehydrogenase(DLD)protein is a post-pyruvate metabolic enzyme,exhibiting reactive oxygen species(ROS)production which causes capacitation.Additionally,other vital functions of DLD in buffalo spermatozoa are hyperactivation and acrosome reaction.DLD produces the optimum amount of ROS required to induce capacitation process in FRT.Depending on physiological or patho-physiological conditions,DLD can either enhance or attenuate the production of reactive oxygen species(ROS).Aim of this study was to investigate whether changes in the production of ROS in sperm cells can impact their ability to fertilize by triggering the capacitation and acrosome reaction.Results In this study,abundance of DLD protein was quantified between high(n=5)and low fertile bull(n=5)sper-matozoa.It was found that compared to high-fertile(HF)bulls,low-fertile(LF)bulls exhibited significantly(P<0.05)higher DLD abundances.Herein,we optimised the MICA concentration to inhibit DLD function,spermatozoa were treated with MICA in time(0,1,2,3,4,and 5 h)and concentrations(1,2.5,5,and 10 mmol/L)dependent manner.Maximum DLD inhibition was found to be at 4 h in 10 mmol/L MICA concentration,which was used for further exper-imentation in HF and LF.Based on DLD inhibition it was seen that LF bull spermatozoa exhibited significantly(P<0.05)higher ROS production and acrosome reaction in comparison to the HF bull spermatozoa.The kinematic parameters of the spermatozoa such as percent total motility,velocity parameters(VCL,VSL,and VAP)and other parameters(BCF,STR,and LIN)were also decreased in MICA treated spermatozoa in comparison to the control(capacitated)spermatozoa.Conclusions The present study provides an initial evidence explaining the buffalo bull spermatozoa with higher DLD abundance undergo early capacitation,which subsequently reduces their capacity to fertilize.展开更多
Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-c...Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-cropping system, 4 main plots （fertilization levels） and 6 subplots （planting densities） were set in a split plot design. The results suggested that yield had close relationship with fertilization levels and planting densities. Different fertilization levels and planting densities significantly affected yield. With the increase of nitrogen fertilization, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency declined. Under the same fertilization level, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency grew a little with the increase of planting density, so nitrogen efficiency could be improved by regulating planting density. The results also showed that A2 （including N 225.0 kg/hm2, P205 75.0 kg/hm^2, K20 187.5 kg/hm^2） matching to B3 （52 500 plants/hm^2） or B4（60 000 plants/hm^2） was a better design, which could obtain a higher yield in the range of 7 913.2-8 207.8 kg/hm2, respectively.展开更多
The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Ferti...The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Fertilizer rates of K2O 135 and 270 kg/hm2, representing 1x and 2x recommended K rates, were applied, no application of k fertilizer as the CK. The results show that the lint yield increased 39.13%-57.48%with potassium application, highly significantly. Al yield components of the three hy-brid cotton varieties increased with the increase of K application amounts. The bol number per plant, single bol weight and lint percentage were increased by 14.24%-40.29%, 3.59%-15.51% and 0.16%-4.89%, respectively, and the fiber length and specific strength also increased with the increasing K application amounts, showing no significant influence on Micronaire. When the K application amounts increased from 135 to 270 kg/hm2, the partial factor productivity （PFPk） reduced by 45.93%-48.01%, and the agronomic efficiency （AEk） reduced by 37.1%-42.9%. The PFPk and KE （K efficiency coefficients） of S328 were the highest among the three varieties, which also showed the strongest resistance to low potassium stress, and with no potassi-um fertilizer application （K0）, the lint yield of S328 was 5.54% and 11.19% higher than that of X8 and J102. The AEk of J102 was the highest, and its reward of K fertilizer was the greatest among the three varieties.展开更多
[Objective] The paper was to study the nitrogen application amount and nitrogen application model for high grade hybrid rapeseed （Brassica napus L.） to get high yield. [Method] With ＂Youyan 599＂ and ＂Sanbei 98＂ ...[Objective] The paper was to study the nitrogen application amount and nitrogen application model for high grade hybrid rapeseed （Brassica napus L.） to get high yield. [Method] With ＂Youyan 599＂ and ＂Sanbei 98＂ as materials, using quadratic regression orthogonal gyration combination design, the impact of nitrogen application amount during various periods on rapeseed yield was studied. [Result] The combinations of factors to obtain the highest yield index （2 898.211 kg / hm 2 ） of ＂Youyan 599＂ were as follows： living rape fertilizer 89.27 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 101.12 kg / hm 2 , total nitrogen application amount 310.39 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12th lunar month fertilizer were 28.76%, 38.66% and 32.58%, respectively. The combinations of factors to obtain the highest yield index （2 870.14 kg/hm 2 ） of ＂Sanbei 98＂ were as follows： living rape fertilizer 120 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 37.55kg / hm 2 , total nitrogen application amount 277.55 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 43.24%, 43.24% and 13.53%, respectively. The combinations of factors to obtain the highest yield index of two combined varieties （2 813.82 kg/hm 2 ）were as follows： living rape fertilizer 120 kg/hm 2 , opening fertilizer 120 kg/hm 2 , 12 th lunar month fertilizer 76.23 kg/hm 2 , total nitrogen application amount 316.23 kg/hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 37.95% , 37.95% and 24.11% , respectively. [Conclusion] The paper provided theoretical basis for high yield cultivation of high grade hybridized rapeseed.展开更多
In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filmin...In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filming, fertilizing and sowing on one for foxtail millet was formed through the integration of plastic film mulching technology and mechanized production technology by Institute of Millet crops of Hebei Academy of Agriculture and Forestry Sciences, and the techniques were introduced from the key technologies of pre-sowing preparation, sowing, supporting equipment, field management, harvesting, plastic film recycling.展开更多
[Objective] This study aimed to provide certain scientific basis for reasonable application of nitrogen fertilizer in the pollution-free cultivation of Phaseolus vulgaris. [Method] A field experiment was conducted to ...[Objective] This study aimed to provide certain scientific basis for reasonable application of nitrogen fertilizer in the pollution-free cultivation of Phaseolus vulgaris. [Method] A field experiment was conducted to investigate the effects of different nitrogen fertilization amounts on the prophase yield and economic efficiency of P. vulgaris and available nitrogen content of soil. [Result] With the increased application amount of nitrogen fertilizer, the prophase yield and economic efficiency of P. vulgaris reached the peaks in the Treatment 3 （8 690.48 kg/ hm^2 and 32 222 yuan/hm^2）, and significant differences were found among different treatment groups. With the increased nitrogen fertilization amount, the soil available nitrogen content increased, showing a positive correlation with correlation coefficient of 0.856 5. Excess nitrogen fertilizer reduced the prophase yield and economic efficiency of P. vulgaris. For the open field cultivation of P. vulgaris, the optimum application amount of nitrogen fertilizer was 178 kg/hm^2. [Conclusion] Application of nitrogen fertilizer could effectively increase the soil available nitrogen content.展开更多
[Objective] This study was conducted to optimize the suitable application amounts of nitrogen fertilizer and phosphate fertilizer for winter wheat.[Method] A field experiment was carried out to investigate the effects...[Objective] This study was conducted to optimize the suitable application amounts of nitrogen fertilizer and phosphate fertilizer for winter wheat.[Method] A field experiment was carried out to investigate the effects of the application amounts of nitrogen fertilizer and phosphate fertilizer on the yield of winter wheat.[Result]The quadratic simulation function between the application of nitrogen fertilizer and wheat yield was y =-0.6611x^2＋20.091 x ＋234.85,with a correlation coefficient of0.970 8,and the yield of winter wheat was the highest at the application amount of nitrogen fertilizer of 228.0 kg/hm^2.The quadratic simulation function between the application of phosphate fertilizer and wheat yield was y =-0.572 6x^2＋13.168 x ＋340.4,with a correlation coefficient of 0.921 95,and the yield of winter wheat was the highest at the application amount of phosphate fertilizer of 172.5 kg/hm^2.[Conclusion] This study provides a scientific basis for the rational application of nitrogen fertilizer and phosphate fertilizer on winter wheat.展开更多
The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger ...The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger systems such as the cAMP/adenylate cyclase （AC） system and the cGMP/guanylate cyclase （GC） system appear to regulate sperm functions. Increased levels of intracytosolic cAMP result in an enhancement of sperm motility and viability. The stimulation of GC by low doses of nitric oxide （NO） leads to an improvement or maintenance of sperm motility, whereas higher concentrations have an adverse effect on sperm parameters. Several in vivo and in vitro studies have been carried out in order to examine whether PDE5 inhibitors affect positively or negatively sperm parameters and sperm fertilizing capacity. The results of these studies are controversial. Some of these studies demonstrate no significant effects of PDE5 inhibitors on the motility, viability, and morphology of spermatozoa collected from men that have been treated with PDE5 inhibitors. On the other hand, several studies demonstrate a positive effect of PDE5 inhibitors on sperm motility both in vivo and in vitro. In vitro studies of sildenafil citrate demonstrate a stimulatory effect on sperm motility with an increase in intracellular cAMP suggesting an inhibitory action of sildenafil citrate on a PDE isoform other than the PDE5. On the other hand, tadalafil＇s actions appear to be associated with the inhibitory effect of this compound on PDE11. In vivo studies in men treated with vardenafil in a daily basis demonstrated a significantly larger total number of spermatozoa per ejaculate, quantitative sperm motility, and qualitative sperm motility; it has been suggested that vardenafil administration enhances the secretory function of the prostate and subsequently increases the qualitative and quantitative motility of spermatozoa. The effect that PDE5 inhibitors exert on sperm parameters may lead to the improvement of the outcome of assisted reproductive technology （ART） programs. In the future PDE5 inhibitors might serve as adjunct therapeutical agents for the alleviation of male infertility.展开更多
Phosphorus （P） is one of the most widely occurring nutrients for development and growth of wheat. In this study, the effects of P application amount on grain yield, protein content, and phosphorus use efficiency （...Phosphorus （P） is one of the most widely occurring nutrients for development and growth of wheat. In this study, the effects of P application amount on grain yield, protein content, and phosphorus use efficiency （PUE） were studied by agronomic management of P fertilizer on spring weak-gluten wheat （Triticum aestivum L.） grown under field conditions for 2 yr. The experiments were performed at five levels of P205 application amount, including 0, 72, 108, 144, and 180 kg ha-1. As a result, with increase in P fertilizer, grain yield, and P agricultural efficiency （AEp） increased in a quadratic equitation, but partial factor productivity of P （PFPp） decreased in a logarithmic eq. When 108 kg ha-1 P2Os was applied, the grain yield reached the highest level, but the protein content in gain was lower than 11.5%, a threshold for the protein content to evaluate weak-gluten wheat suitable for production of cake and biscuit. Yangmai 13 and Ningmai 9 could tolerate to higher P level of soils than Yangmai 9 that had more loss in grain yield when P fertilizer was over-applied. AEp had a concomitant relationship with grain yield and was a better descriptor for P use efficiency in the wheat. A high P use efficiency resulted in leaf area index （LAI）, increased chlorophyll content and photosynthetic rate, and stable acid phophatase （APase） activity to accumulate more dry matter after anthesis, which explained that the optimum P fertilizer increased grain yield and improved grain quality of weak-gluten wheat.展开更多
Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gam...Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gametogenesis. Less is known about the effects cholesterol homeostasis may have on postgonadic reproductive functions. Lately, several data have been reported showing how imbalanced cholesterol levels may particularly affect the post-testicular events of sperm maturation that lead to fully fertile male gametes. This review will focus on that aspect and essentially centers on how cholesterol is important for the physiology of the mammalian epididymis and spermatozoa.展开更多
Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of...Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances （EPS） from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To investigate the impact of EPS on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal status, hypo-osmotic swelling, and in vitro fertilization rate after incubating the sperm with EPS in vitro using mice. The incubation of sperm with EPS significantly decreased sperm motility, viability, and functional integrity in a concentration and time-dependent manner. These effects on sperm quality also resulted in a decreased fertilization rate in vitro. This is the first report that demonstrates the direct negative impact of the EPS of T. vaginalis on the fertilization rate of sperm in vitro. However, further study should be performed using human sperm to determine if EPS has similar negative impact on human sperm fertilizing capacity in vitro.展开更多
This study aimed to investigate the effects of reducing application amount of base fertilizer and increasing application time of leaf fertilizer on corn yield so as to find out the most economical fertilization way fo...This study aimed to investigate the effects of reducing application amount of base fertilizer and increasing application time of leaf fertilizer on corn yield so as to find out the most economical fertilization way for corn. On the basis of protecting environment, the economic benefits will be also increased. The results showed that the corn yield was increased with the increase of application amount of base fertilizer, and was also increased with the increase of application time of leaf fertilizer.For each time of spaying of leaf fertilizer, the corn yield was increased 258-592.5kg/hm^2 with increase amplitude ranging from 2.3%-5.6%.展开更多
In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication o...In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.展开更多
In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents ...In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.展开更多
The activity of microorganisms in the soil can be adjusted through the application of stabilized fertilizer.Using biochemical means,the transformation of nitrogen in the soil is controlled,synchronizing the peak of nu...The activity of microorganisms in the soil can be adjusted through the application of stabilized fertilizer.Using biochemical means,the transformation of nitrogen in the soil is controlled,synchronizing the peak of nutrient released from soil and the peak of nutrient absorption by rice.With the application of stabilized fertilizer,the fertilizer utilization rate and the income of farmers can be improved and the environmental pollution can be cut down by reducing the amount of nitrogen fertilizer and decreasing the frequency of fertilization.This paper summarizes the application effects of stabilized fertilizer on different cultivars of rice in different regions for many years,and puts forward the issues to be concerned in the application of stabilized fertilizer with reduced amount and frequency and efficiency improvement techniques.展开更多
Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of s...Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood.The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in cattle.Results:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and calcium.Insemination of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P<0.01),whereas the rate of sperm with moderate+high single-stranded breaks was higher in the second period(0-2 h:3.52±7.77%/h vs.2-4h:21.06±11.69%/h;P<0.0001).Regarding sperm physiology,viability decrease rate was different between the two periods(0-2 h:-4.49±1.79%/h vs.2-4 h:-2.50±3.39%/h;P=0.032),but the progressive motility decrease rate was constant throughout post-thawing incubation(0-2 h:-4.70±3.42%/h vs.2-4 h:-1.89±2.97%/h;P>0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage and chromatin deprotamination suggested a sequence of events all driven by oxidative stress,where viability and motility would be affected first and sperm chromatin would be altered at a later stage,thus suggesting that bovine sperm should be used for fertilization within 2 h post-thaw.Fertility correlations supported that the assessment of global DNA damage through the Comet assay may help predict bull fertility.展开更多
The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in f...The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.展开更多
[Objectives]This study was conducted to explore the effects of planting density,fertilizer type and fertilizer amount on yield of Jicaidou No.1.[Methods]Three treatments of plant spacing,fertilizer type and fertilizer...[Objectives]This study was conducted to explore the effects of planting density,fertilizer type and fertilizer amount on yield of Jicaidou No.1.[Methods]Three treatments of plant spacing,fertilizer type and fertilizer amount of Jicaidou No.1 were set up respectively to screen out the best combination mode.[Results]The best combination mode was:plant spacing 50 cm,fertilizer amount 1800 kg/hm^(2),and any one of the two fertilizers,as the difference between the two kinds of fertilizers was not significant.[Conclusions]This study provides a scientific basis for the standardized cultivation of kidney beans in Jilin Province.展开更多
基金Supported by the National Science Found for Young Scholars of China(No.31101123)Natural Science Foundation of Hainan Province of China(No.311062)~~
文摘Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hainan Province. The results showed that the optimal fertilizing amount of ternary quadratic model simulation was0.374 kg/plant of N, 0.289 kg/plant of P2O5 and 0.891 kg/plant of K2 O. According to the yield trend characteristic, the optimal fertilizing amount of unary quadratic model was 0.400kg/plant of N, 0.214 kg/plant of P2O5 and 0.901kg/plant of K2 O. Thus it can be seen that only partial indices of the optimal fertilizing amount of ternary quadratic model simulation were higher than that of unary quadratic model. Considering the results, the optimal fertilizing amount of Brazil banana was 0.374-0.400kg/plant of N, 0.214-0.289 kg/plant of P2O5 and 0.891-0.901 kg/plant of K2 O.
基金Supported by National Modern Agricultural Technology System for Wheat(CARS-3-2-22)Special Research Fund for National Public Service Sector(Agriculture)(201203033)+1 种基金Modern Agricultural Industry Technology System Project(nycytx-03)Shandong Modern Agricultural Industry Technology System(SDAIT-04-021-12)
文摘To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amount and planting density on yield and quality of material.The results showed that Taishan 27 had high yield under fertilizing amount of 225 kg/ha pure nitrogen and planting density of 240 × 104-300× 10~4/ha; the yield was lowest under fertilizing amount of 300 kg/ha pure nitrogen and planting density of 360 × 10~4/ha. The suitable planting density for Taishan 27 was 240 × 104-300 × 10~4/ha,and the fertilizing amount of nitrogen should be based on different soil fertility conditions to avoid water and fertilizer stress and improve yield.
基金Bill&Melinda Gates Foundation(Grant number OPP1154401).
文摘Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place as the spermatozoa pass through the female reproductive tract(FRT).Dihydrolipoamide dehydrogenase(DLD)protein is a post-pyruvate metabolic enzyme,exhibiting reactive oxygen species(ROS)production which causes capacitation.Additionally,other vital functions of DLD in buffalo spermatozoa are hyperactivation and acrosome reaction.DLD produces the optimum amount of ROS required to induce capacitation process in FRT.Depending on physiological or patho-physiological conditions,DLD can either enhance or attenuate the production of reactive oxygen species(ROS).Aim of this study was to investigate whether changes in the production of ROS in sperm cells can impact their ability to fertilize by triggering the capacitation and acrosome reaction.Results In this study,abundance of DLD protein was quantified between high(n=5)and low fertile bull(n=5)sper-matozoa.It was found that compared to high-fertile(HF)bulls,low-fertile(LF)bulls exhibited significantly(P<0.05)higher DLD abundances.Herein,we optimised the MICA concentration to inhibit DLD function,spermatozoa were treated with MICA in time(0,1,2,3,4,and 5 h)and concentrations(1,2.5,5,and 10 mmol/L)dependent manner.Maximum DLD inhibition was found to be at 4 h in 10 mmol/L MICA concentration,which was used for further exper-imentation in HF and LF.Based on DLD inhibition it was seen that LF bull spermatozoa exhibited significantly(P<0.05)higher ROS production and acrosome reaction in comparison to the HF bull spermatozoa.The kinematic parameters of the spermatozoa such as percent total motility,velocity parameters(VCL,VSL,and VAP)and other parameters(BCF,STR,and LIN)were also decreased in MICA treated spermatozoa in comparison to the control(capacitated)spermatozoa.Conclusions The present study provides an initial evidence explaining the buffalo bull spermatozoa with higher DLD abundance undergo early capacitation,which subsequently reduces their capacity to fertilize.
文摘Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-cropping system, 4 main plots （fertilization levels） and 6 subplots （planting densities） were set in a split plot design. The results suggested that yield had close relationship with fertilization levels and planting densities. Different fertilization levels and planting densities significantly affected yield. With the increase of nitrogen fertilization, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency declined. Under the same fertilization level, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency grew a little with the increase of planting density, so nitrogen efficiency could be improved by regulating planting density. The results also showed that A2 （including N 225.0 kg/hm2, P205 75.0 kg/hm^2, K20 187.5 kg/hm^2） matching to B3 （52 500 plants/hm^2） or B4（60 000 plants/hm^2） was a better design, which could obtain a higher yield in the range of 7 913.2-8 207.8 kg/hm2, respectively.
基金Supported by the Project for Cotton Industry of Hunan Province(Xiangnongyelian[2012]No.278)the Foundation for Talents Stabilization of Hunan Agricultural University(09WD19)~~
文摘The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Fertilizer rates of K2O 135 and 270 kg/hm2, representing 1x and 2x recommended K rates, were applied, no application of k fertilizer as the CK. The results show that the lint yield increased 39.13%-57.48%with potassium application, highly significantly. Al yield components of the three hy-brid cotton varieties increased with the increase of K application amounts. The bol number per plant, single bol weight and lint percentage were increased by 14.24%-40.29%, 3.59%-15.51% and 0.16%-4.89%, respectively, and the fiber length and specific strength also increased with the increasing K application amounts, showing no significant influence on Micronaire. When the K application amounts increased from 135 to 270 kg/hm2, the partial factor productivity （PFPk） reduced by 45.93%-48.01%, and the agronomic efficiency （AEk） reduced by 37.1%-42.9%. The PFPk and KE （K efficiency coefficients） of S328 were the highest among the three varieties, which also showed the strongest resistance to low potassium stress, and with no potassi-um fertilizer application （K0）, the lint yield of S328 was 5.54% and 11.19% higher than that of X8 and J102. The AEk of J102 was the highest, and its reward of K fertilizer was the greatest among the three varieties.
基金Supported by Construction Project of National Rapeseed Modern Industrial Technology System (nycytx-00563)Guizhou Academy of Agricultural Sciences "Research of High Yield and High Quality Cultivation Technology for High Grade Hybrid Rapeseed with High Oil" [C ZX(2007)015]+2 种基金Department of Agriculture of Guizhou Province "Research, Promotion and Application of High Yield Cultivation Technology for Hybridized Rapeseed of New Variety Youyan 599" [QNYZZ (2009) 007]Guizhou Academy of Agricultural Sciences "Large Area Intermediate Experiment, Promotion and Application of Hybridized Rapeseed Youyan 599" [QNKZX (2009) 030]Department of Agriculture of Guizhou Province "Integrated Innovation of Seed Production Techniques and Large Area Demonstration for New High Oil Rapeseed Hybrid Variety Sanbei 98 [QKH NY (2010) 3087]~~
文摘[Objective] The paper was to study the nitrogen application amount and nitrogen application model for high grade hybrid rapeseed （Brassica napus L.） to get high yield. [Method] With ＂Youyan 599＂ and ＂Sanbei 98＂ as materials, using quadratic regression orthogonal gyration combination design, the impact of nitrogen application amount during various periods on rapeseed yield was studied. [Result] The combinations of factors to obtain the highest yield index （2 898.211 kg / hm 2 ） of ＂Youyan 599＂ were as follows： living rape fertilizer 89.27 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 101.12 kg / hm 2 , total nitrogen application amount 310.39 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12th lunar month fertilizer were 28.76%, 38.66% and 32.58%, respectively. The combinations of factors to obtain the highest yield index （2 870.14 kg/hm 2 ） of ＂Sanbei 98＂ were as follows： living rape fertilizer 120 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 37.55kg / hm 2 , total nitrogen application amount 277.55 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 43.24%, 43.24% and 13.53%, respectively. The combinations of factors to obtain the highest yield index of two combined varieties （2 813.82 kg/hm 2 ）were as follows： living rape fertilizer 120 kg/hm 2 , opening fertilizer 120 kg/hm 2 , 12 th lunar month fertilizer 76.23 kg/hm 2 , total nitrogen application amount 316.23 kg/hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 37.95% , 37.95% and 24.11% , respectively. [Conclusion] The paper provided theoretical basis for high yield cultivation of high grade hybridized rapeseed.
基金Supported by the National Key Technology R&D Program(2014BAD07B01-02)the Science and Technology Demonstration Project of Bohai Granary in Hebei Provincethe Special Fund for Agro-scientific Research in Public Interest(201303133-1-6)~~
文摘In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filming, fertilizing and sowing on one for foxtail millet was formed through the integration of plastic film mulching technology and mechanized production technology by Institute of Millet crops of Hebei Academy of Agriculture and Forestry Sciences, and the techniques were introduced from the key technologies of pre-sowing preparation, sowing, supporting equipment, field management, harvesting, plastic film recycling.
文摘[Objective] This study aimed to provide certain scientific basis for reasonable application of nitrogen fertilizer in the pollution-free cultivation of Phaseolus vulgaris. [Method] A field experiment was conducted to investigate the effects of different nitrogen fertilization amounts on the prophase yield and economic efficiency of P. vulgaris and available nitrogen content of soil. [Result] With the increased application amount of nitrogen fertilizer, the prophase yield and economic efficiency of P. vulgaris reached the peaks in the Treatment 3 （8 690.48 kg/ hm^2 and 32 222 yuan/hm^2）, and significant differences were found among different treatment groups. With the increased nitrogen fertilization amount, the soil available nitrogen content increased, showing a positive correlation with correlation coefficient of 0.856 5. Excess nitrogen fertilizer reduced the prophase yield and economic efficiency of P. vulgaris. For the open field cultivation of P. vulgaris, the optimum application amount of nitrogen fertilizer was 178 kg/hm^2. [Conclusion] Application of nitrogen fertilizer could effectively increase the soil available nitrogen content.
文摘[Objective] This study was conducted to optimize the suitable application amounts of nitrogen fertilizer and phosphate fertilizer for winter wheat.[Method] A field experiment was carried out to investigate the effects of the application amounts of nitrogen fertilizer and phosphate fertilizer on the yield of winter wheat.[Result]The quadratic simulation function between the application of nitrogen fertilizer and wheat yield was y =-0.6611x^2＋20.091 x ＋234.85,with a correlation coefficient of0.970 8,and the yield of winter wheat was the highest at the application amount of nitrogen fertilizer of 228.0 kg/hm^2.The quadratic simulation function between the application of phosphate fertilizer and wheat yield was y =-0.572 6x^2＋13.168 x ＋340.4,with a correlation coefficient of 0.921 95,and the yield of winter wheat was the highest at the application amount of phosphate fertilizer of 172.5 kg/hm^2.[Conclusion] This study provides a scientific basis for the rational application of nitrogen fertilizer and phosphate fertilizer on winter wheat.
文摘The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger systems such as the cAMP/adenylate cyclase （AC） system and the cGMP/guanylate cyclase （GC） system appear to regulate sperm functions. Increased levels of intracytosolic cAMP result in an enhancement of sperm motility and viability. The stimulation of GC by low doses of nitric oxide （NO） leads to an improvement or maintenance of sperm motility, whereas higher concentrations have an adverse effect on sperm parameters. Several in vivo and in vitro studies have been carried out in order to examine whether PDE5 inhibitors affect positively or negatively sperm parameters and sperm fertilizing capacity. The results of these studies are controversial. Some of these studies demonstrate no significant effects of PDE5 inhibitors on the motility, viability, and morphology of spermatozoa collected from men that have been treated with PDE5 inhibitors. On the other hand, several studies demonstrate a positive effect of PDE5 inhibitors on sperm motility both in vivo and in vitro. In vitro studies of sildenafil citrate demonstrate a stimulatory effect on sperm motility with an increase in intracellular cAMP suggesting an inhibitory action of sildenafil citrate on a PDE isoform other than the PDE5. On the other hand, tadalafil＇s actions appear to be associated with the inhibitory effect of this compound on PDE11. In vivo studies in men treated with vardenafil in a daily basis demonstrated a significantly larger total number of spermatozoa per ejaculate, quantitative sperm motility, and qualitative sperm motility; it has been suggested that vardenafil administration enhances the secretory function of the prostate and subsequently increases the qualitative and quantitative motility of spermatozoa. The effect that PDE5 inhibitors exert on sperm parameters may lead to the improvement of the outcome of assisted reproductive technology （ART） programs. In the future PDE5 inhibitors might serve as adjunct therapeutical agents for the alleviation of male infertility.
基金the National Natural Science Foundation of China (30971729)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Chinathe Science and Technology Support Program of Jiangsu Province, China (BE2009426)
文摘Phosphorus （P） is one of the most widely occurring nutrients for development and growth of wheat. In this study, the effects of P application amount on grain yield, protein content, and phosphorus use efficiency （PUE） were studied by agronomic management of P fertilizer on spring weak-gluten wheat （Triticum aestivum L.） grown under field conditions for 2 yr. The experiments were performed at five levels of P205 application amount, including 0, 72, 108, 144, and 180 kg ha-1. As a result, with increase in P fertilizer, grain yield, and P agricultural efficiency （AEp） increased in a quadratic equitation, but partial factor productivity of P （PFPp） decreased in a logarithmic eq. When 108 kg ha-1 P2Os was applied, the grain yield reached the highest level, but the protein content in gain was lower than 11.5%, a threshold for the protein content to evaluate weak-gluten wheat suitable for production of cake and biscuit. Yangmai 13 and Ningmai 9 could tolerate to higher P level of soils than Yangmai 9 that had more loss in grain yield when P fertilizer was over-applied. AEp had a concomitant relationship with grain yield and was a better descriptor for P use efficiency in the wheat. A high P use efficiency resulted in leaf area index （LAI）, increased chlorophyll content and photosynthetic rate, and stable acid phophatase （APase） activity to accumulate more dry matter after anthesis, which explained that the optimum P fertilizer increased grain yield and improved grain quality of weak-gluten wheat.
文摘Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gametogenesis. Less is known about the effects cholesterol homeostasis may have on postgonadic reproductive functions. Lately, several data have been reported showing how imbalanced cholesterol levels may particularly affect the post-testicular events of sperm maturation that lead to fully fertile male gametes. This review will focus on that aspect and essentially centers on how cholesterol is important for the physiology of the mammalian epididymis and spermatozoa.
文摘Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances （EPS） from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To investigate the impact of EPS on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal status, hypo-osmotic swelling, and in vitro fertilization rate after incubating the sperm with EPS in vitro using mice. The incubation of sperm with EPS significantly decreased sperm motility, viability, and functional integrity in a concentration and time-dependent manner. These effects on sperm quality also resulted in a decreased fertilization rate in vitro. This is the first report that demonstrates the direct negative impact of the EPS of T. vaginalis on the fertilization rate of sperm in vitro. However, further study should be performed using human sperm to determine if EPS has similar negative impact on human sperm fertilizing capacity in vitro.
文摘This study aimed to investigate the effects of reducing application amount of base fertilizer and increasing application time of leaf fertilizer on corn yield so as to find out the most economical fertilization way for corn. On the basis of protecting environment, the economic benefits will be also increased. The results showed that the corn yield was increased with the increase of application amount of base fertilizer, and was also increased with the increase of application time of leaf fertilizer.For each time of spaying of leaf fertilizer, the corn yield was increased 258-592.5kg/hm^2 with increase amplitude ranging from 2.3%-5.6%.
文摘In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2016YFD0101603)Fundamental Research Funds for the Central Universities in 2016(XDJK2016A020)
文摘In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.
基金National Key Research and Development Program of China(2017YFD0200708)Science and Technology Plan Project of Shenyang City(18-202-0-09).
文摘The activity of microorganisms in the soil can be adjusted through the application of stabilized fertilizer.Using biochemical means,the transformation of nitrogen in the soil is controlled,synchronizing the peak of nutrient released from soil and the peak of nutrient absorption by rice.With the application of stabilized fertilizer,the fertilizer utilization rate and the income of farmers can be improved and the environmental pollution can be cut down by reducing the amount of nitrogen fertilizer and decreasing the frequency of fertilization.This paper summarizes the application effects of stabilized fertilizer on different cultivars of rice in different regions for many years,and puts forward the issues to be concerned in the application of stabilized fertilizer with reduced amount and frequency and efficiency improvement techniques.
基金the European Union’s Horizon 2020 Research and Innovation scheme under the Marie Sklodowska-Curie grant agreement No.801342(Tecniospring INDUSTRY,TECSPR-19-1-0003)the Ministry of Science and Innovation,Spain(AGL2017-88329-R and PID2020-113320RBI00)+2 种基金the Catalan Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(2017-SGR-1229)the Catalan Institution for Research and Advanced Studies(ICREA)La Maratóde TV3 Foundation(214/857-202039)。
文摘Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood.The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in cattle.Results:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and calcium.Insemination of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P<0.01),whereas the rate of sperm with moderate+high single-stranded breaks was higher in the second period(0-2 h:3.52±7.77%/h vs.2-4h:21.06±11.69%/h;P<0.0001).Regarding sperm physiology,viability decrease rate was different between the two periods(0-2 h:-4.49±1.79%/h vs.2-4 h:-2.50±3.39%/h;P=0.032),but the progressive motility decrease rate was constant throughout post-thawing incubation(0-2 h:-4.70±3.42%/h vs.2-4 h:-1.89±2.97%/h;P>0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage and chromatin deprotamination suggested a sequence of events all driven by oxidative stress,where viability and motility would be affected first and sperm chromatin would be altered at a later stage,thus suggesting that bovine sperm should be used for fertilization within 2 h post-thaw.Fertility correlations supported that the assessment of global DNA damage through the Comet assay may help predict bull fertility.
文摘The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.
文摘[Objectives]This study was conducted to explore the effects of planting density,fertilizer type and fertilizer amount on yield of Jicaidou No.1.[Methods]Three treatments of plant spacing,fertilizer type and fertilizer amount of Jicaidou No.1 were set up respectively to screen out the best combination mode.[Results]The best combination mode was:plant spacing 50 cm,fertilizer amount 1800 kg/hm^(2),and any one of the two fertilizers,as the difference between the two kinds of fertilizers was not significant.[Conclusions]This study provides a scientific basis for the standardized cultivation of kidney beans in Jilin Province.
