Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms...Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms of DEK in treating AR,aiming to identify potential new treatment targets for AR.Methods:The AR mouse model was induced by house dust mite(HDM)(1 mg/m L).HNEp Cs stimulated by HDM(1 mg/m L)were pretreated for 24 h with or without DEK lentivirus.The effect of DEK knockout or knockdown on AR was evaluated in vitro and in vivo using western blotting,ELISA,flow cytometry,real-time quantitative PCR,immunohistochemistry,HE staining,PAS staining,Diff staining,and immunofluorescence.Results:After DEK knockdown,the inflammatory response of AR mice was reduced.In addition,DEK deletion mitigated nasal tissue damage and mitochondrial division.Our further studies showed that DEK deletion or inhibition led to the down-regulation of Rho A activity and decreased phosphorylation of Ezrin and Drp1 proteins,and inhibited mitochondrial division.Overall,DEK deficiency mitigated AR by down-regulating Rho A/Ezrin/Drp1 pathway activity.Conclusion:DEK alleviates AR through Rho A/Ezrin/Drp1 signaling pathway,which provides a new perspective for developing improved therapies and understanding the pathogenesis of AR.展开更多
Ezrin,as a key connecting protein between the cytoskeleton and cell membrane,plays an important role in various cellular physiological processes.In recent years,people have gradually attached importance to its researc...Ezrin,as a key connecting protein between the cytoskeleton and cell membrane,plays an important role in various cellular physiological processes.In recent years,people have gradually attached importance to its research in the field of glucose and lipid metabolism.Based on relevant research materials,this article elaborates the structure and function of Ezrin,and focuses on its role and potential mechanism in the diseases related to abnormal glucose and lipid metabolism,such as diabetes and its complications,to provide new ideas and theoretical basis for in-depth understanding of the regulation of glucose and lipid metabolism and the prevention and treatment of related diseases.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:82260007 and 82260218The Department of Education of Jilin Province,(JJKH20240698KJ)+1 种基金Natural Science Research Foundation of Jilin Province for Sciences and Technology,Grant/Award Number:20210101215JC20240602100RC and 20240404025ZP。
文摘Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms of DEK in treating AR,aiming to identify potential new treatment targets for AR.Methods:The AR mouse model was induced by house dust mite(HDM)(1 mg/m L).HNEp Cs stimulated by HDM(1 mg/m L)were pretreated for 24 h with or without DEK lentivirus.The effect of DEK knockout or knockdown on AR was evaluated in vitro and in vivo using western blotting,ELISA,flow cytometry,real-time quantitative PCR,immunohistochemistry,HE staining,PAS staining,Diff staining,and immunofluorescence.Results:After DEK knockdown,the inflammatory response of AR mice was reduced.In addition,DEK deletion mitigated nasal tissue damage and mitochondrial division.Our further studies showed that DEK deletion or inhibition led to the down-regulation of Rho A activity and decreased phosphorylation of Ezrin and Drp1 proteins,and inhibited mitochondrial division.Overall,DEK deficiency mitigated AR by down-regulating Rho A/Ezrin/Drp1 pathway activity.Conclusion:DEK alleviates AR through Rho A/Ezrin/Drp1 signaling pathway,which provides a new perspective for developing improved therapies and understanding the pathogenesis of AR.
文摘Ezrin,as a key connecting protein between the cytoskeleton and cell membrane,plays an important role in various cellular physiological processes.In recent years,people have gradually attached importance to its research in the field of glucose and lipid metabolism.Based on relevant research materials,this article elaborates the structure and function of Ezrin,and focuses on its role and potential mechanism in the diseases related to abnormal glucose and lipid metabolism,such as diabetes and its complications,to provide new ideas and theoretical basis for in-depth understanding of the regulation of glucose and lipid metabolism and the prevention and treatment of related diseases.
