The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the meth...The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the method for simultaneous enumeration of diatoms,protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40.We conducted the evaluation with a sample size of 8.3 ml each from sandy,muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007.The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates,indicating a reliable enumeration for eukaryotic microbenthos,but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos,97.9% for nematodes and 97.8% for copepods from sands,muddy sands and mud.By contrast,the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle,and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos:89.4±2.0% from sands,93±4.1% from muddy sands and 97.1±3.0% from mud.Apart from the sediment type,sample volume was another important factor affecting the extraction efficiency for meiobenthos.The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample.Besides the excellent extraction efficiency,the Ludox-QPS method obtained higher abundances of meiobenthos,in particular nematodes,than the routine Ludox centrifugation,which frequently resulted in an uncertain loss of small meiobenthos during the sieving process.Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation,showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom,protozoa and meiobenthos.Moreover,the comparatively high taxonomic resolution of the method,especially for diatoms and ciliates,makes it feasible to investigate microbial ecology at community level.展开更多
AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality ...AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM), and to evaluate its efficacy. METHODS: Eighty-four patients with postoperative complications or death and 172 patients without postoperative complications, who underwent surgery in our department during the previous 2 years, were retrospectively analyzed by logistic regression. Fifteen indexes were investigated including age, cardiovascular function, respiratory function, blood test results, endocrine function, central nervous system function, hepatic function, renal function, nutritional status, extent of operative trauma, and course of anesthesia. Modified POSSUM (M-POSSUM) was developed using significant risk factors with its efficacy evaluated. RESULTS: The significant risk factors were found to be age, cardiovascular function, respiratory function, hepatic function, renal function, blood test results, endocrine function, nutritional status, duration of operation, intraoperative blood loss, and course of anesthesia. These factors were all included in the scoring system. There were significant differences in the scores between the patients with and without postoperative complications, between the patients died and survived with complications, and between the patients died and survived without complications. The receiver operating characteristic curves showed that the M-POSSUM could accurately predict postoperative complications and mortality.CONCLUSION: M-POSSUM correlates well with postoperative complications and mortality, and is more accurate than POSSUM.展开更多
As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learn...As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learning method is presented for counting the colony forming units(CFUs),which is referred to as CFUCounter.This cellcounting program processes digital images and segments bacterial colonies.The algorithm combines unsupervised machine learning,iterative adaptive thresholding,and local-minima-based watershed segmentation to enable an accurate and robust cell counting.Compared to a manual counting method,CFUCounter supports color-based CFU classification,allows plates containing heterologous colonies to be counted individually,and demonstrates overall performance(slope 0.996,SD 0.013,95%CI:0.97–1.02,p value<1e-11,r=0.999)indistinguishable from the gold standard of point-and-click counting.This CFUCounter application is open-source and easy to use as a unique addition to the arsenal of colony-counting tools.展开更多
Multiple-Input Multiple-Output (MIMO) techniques are promising in wireless communication systems for its high spectral efficiency. Sphere Detector (SD) is favoured in MIMO detection to achieve Maximum-Likelihood (ML) ...Multiple-Input Multiple-Output (MIMO) techniques are promising in wireless communication systems for its high spectral efficiency. Sphere Detector (SD) is favoured in MIMO detection to achieve Maximum-Likelihood (ML) performance. In this paper, we proposed a new SD method for MIMO-Orthogonal Frequency Division Multiplexing (OFDM) systems based on IEEE802.11n, which uses Singular Value Decomposition (SVD) in complex domain to reduce the computation complexity. Furthermore, a new Schnorr-Euchner (SE) enumeration algorithm is also discussed in detail. The computer simulation result shows that the computational complexity and the number of visited nodes can be reduced significantly compared with conventional SD detectors with the same Bit Error Rate (BER) performance.展开更多
The number of configurations, c（n, m）, of a single chain with length n attached to a flat surface with m monomers contacting the surface is exactly enumerated. A fimction of c（n, m） about m and n is obtained. From...The number of configurations, c（n, m）, of a single chain with length n attached to a flat surface with m monomers contacting the surface is exactly enumerated. A fimction of c（n, m） about m and n is obtained. From the function, a scaling law for mean energy of chain is derived, and we estimate the critical point εc = 0.276 and the crossover exponent φ = 0.5. The free energy difference between tethered chain and free chain in dilute solution is also studied, which shows the critical adsorption point is about 0.272 for infinite long chain with φ= 0.5.展开更多
The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disin...The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disintegration involve identifying critical sets of nodes or edges, limited research has been carried out on edge-based disintegration strategies. We propose a novel algorithm, i.e., a rank aggregation elite enumeration algorithm based on edge-coupled networks(RAEEC),which aims to implement tiling for edge-coupled networks by finding important sets of edges in the network while balancing effectiveness and efficiency. Our algorithm is based on a two-layer edge-coupled network model with one-to-one links, and utilizes three advanced edge importance metrics to rank the edges separately. A comprehensive ranking of edges is obtained using a rank aggregation approach proposed in this study. The top few edges from the ranking set obtained by RAEEC are then used to generate an enumeration set, which is continuously iteratively updated to identify the set of elite attack edges.We conduct extensive experiments on synthetic networks to evaluate the performance of our proposed method, and the results indicate that RAEEC achieves a satisfactory balance between efficiency and effectiveness. Our approach represents a significant contribution to the field of network disintegration, particularly for edge-based strategies.展开更多
In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonia...In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonian graphs. Some computational results obtained by microcomputers are listed.展开更多
During the late incubation period or initial phase of dengue virus infection,laboratory confirmation is through viral isolation in cell culture and/or molecular investigations, or immunofluorescence,or immunohistochem...During the late incubation period or initial phase of dengue virus infection,laboratory confirmation is through viral isolation in cell culture and/or molecular investigations, or immunofluorescence,or immunohistochemistry[1].The dengue virus non-structural antigen NSl that would develop before the appearance of dengue IgM and/or IgG is emerging as a suitable option for dengue diagnosis[2].Platelet therapy is a standard clinical practice for dengue patients with severe thrombocytopenia[3].However,during introductory screening,platelet count is not being done in many cases. This results in delays of platelet therapy. In the course of the current(2010) spurt of dengue in New Delhi[4],simultaneous screening for NSl,IgM and IgG and platelet enumeration has been introduced at the展开更多
The graphtheory method is used to enumerate the numbers of constitutions, configurations,chiral and achiral configurations of substituted crown ethers.The numerical results are tabulated.
INTRODUCTION Fecal coliform has been used as an indicator of water pollution for about a century. Recently, Standrige et al (1981), Reasoner et al (1979) and Pagel et al. (1982) improved, with good results, the bioass...INTRODUCTION Fecal coliform has been used as an indicator of water pollution for about a century. Recently, Standrige et al (1981), Reasoner et al (1979) and Pagel et al. (1982) improved, with good results, the bioassy methods for fecal coliform in drinking water and waste water. To provide a method appli-展开更多
A combinatorial method based on the determination of the averaged weight of permutations controlling the chirality/achirality fittingness of 2n substitution sites of the monocyclic cycloalkane allows to obtain general...A combinatorial method based on the determination of the averaged weight of permutations controlling the chirality/achirality fittingness of 2n substitution sites of the monocyclic cycloalkane allows to obtain generalized functional equations for direct enumeration of enantiomers pairs and achiral skeletons of any derivatives of monocyclic cycloalkanes having heteromorphic alkyl substituents with the distinct length k with the empirical formula , wherein at least two alkyl groups??of the distinct size ?each. ?is the number of alkyl radicals ?of the system??verifying the relation . The integer sequences of enantiomer pairs and achiral skeletons are given for substituted derivatives of monocyclic cycloalkane for n = 3, 4 and k = 3, 4, 5. The composite stereoisomerism of this particular compound is also highlighted.展开更多
Simple formulas for the number of different cyclic and dihedral necklaces containing nj beads of the j-th color, and , are derived, using the Pólya enumeration theorem.
Lactic acid bacteria （LAB） are widely used in food industries. Correct identification and safety evaluation of these bacteria at the species even strain level should take considerations into account. In this study, ...Lactic acid bacteria （LAB） are widely used in food industries. Correct identification and safety evaluation of these bacteria at the species even strain level should take considerations into account. In this study, the LAB were recovered from yoghurt and characterized phenotypically and genetically. Fifty-two isolates of LAB from 31 yoghurt samples were cultured and grouped into 6 species including Luctobucillus bulguricus （24 isolates）, Streptococcus thermophilus （15 isolates）, L. ucidophilus （7 isolates）, L. porucusei/cusei （3 isolates）, L. delbrueckii （2 isolates）, and L. fermentum （1 isolate）, based on their Gram-staining, colony morphology and biochemical properties.展开更多
A new combinatorial interpretation of Raney numbers is proposed. We apply this combinatorial interpretation to solve several tree enumeration counting problems. Further a generalized Catalan triangle is introduced and...A new combinatorial interpretation of Raney numbers is proposed. We apply this combinatorial interpretation to solve several tree enumeration counting problems. Further a generalized Catalan triangle is introduced and some of its properties are proved.展开更多
The enumeration of elements of c.e. sets in the theory of computability and computational complexity has already been investigated. However, the order of this enumeration has received less attention. The enumeration o...The enumeration of elements of c.e. sets in the theory of computability and computational complexity has already been investigated. However, the order of this enumeration has received less attention. The enumeration orders of elements of c.e. sets by means of Turing machines on natural numbers are investigated. In this paper, we consider the enumeration orders of elements of c.e. sets on rational numbers. We present enumeration order reducibility and enumeration order equivalence on rational numbers and propose some lemmas and theorems on these concepts. Also, we show that the theories here hold for Rc and we could repeat the same theories in this domain, in a same way.展开更多
A map is singular if each edge is on the same face on a surface (i.e., it has only one face on a surface). In this paper we present the chromatic enumeration for rooted singular maps on the Klein bottle.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No.KZCX2-YW-417)the National Natural Science Foundation of China (No.40576072+1 种基金40706047)the "100 Talents Project" of the Chinese Academy of Sciences
文摘The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the method for simultaneous enumeration of diatoms,protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40.We conducted the evaluation with a sample size of 8.3 ml each from sandy,muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007.The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates,indicating a reliable enumeration for eukaryotic microbenthos,but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos,97.9% for nematodes and 97.8% for copepods from sands,muddy sands and mud.By contrast,the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle,and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos:89.4±2.0% from sands,93±4.1% from muddy sands and 97.1±3.0% from mud.Apart from the sediment type,sample volume was another important factor affecting the extraction efficiency for meiobenthos.The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample.Besides the excellent extraction efficiency,the Ludox-QPS method obtained higher abundances of meiobenthos,in particular nematodes,than the routine Ludox centrifugation,which frequently resulted in an uncertain loss of small meiobenthos during the sieving process.Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation,showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom,protozoa and meiobenthos.Moreover,the comparatively high taxonomic resolution of the method,especially for diatoms and ciliates,makes it feasible to investigate microbial ecology at community level.
文摘AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM), and to evaluate its efficacy. METHODS: Eighty-four patients with postoperative complications or death and 172 patients without postoperative complications, who underwent surgery in our department during the previous 2 years, were retrospectively analyzed by logistic regression. Fifteen indexes were investigated including age, cardiovascular function, respiratory function, blood test results, endocrine function, central nervous system function, hepatic function, renal function, nutritional status, extent of operative trauma, and course of anesthesia. Modified POSSUM (M-POSSUM) was developed using significant risk factors with its efficacy evaluated. RESULTS: The significant risk factors were found to be age, cardiovascular function, respiratory function, hepatic function, renal function, blood test results, endocrine function, nutritional status, duration of operation, intraoperative blood loss, and course of anesthesia. These factors were all included in the scoring system. There were significant differences in the scores between the patients with and without postoperative complications, between the patients died and survived with complications, and between the patients died and survived without complications. The receiver operating characteristic curves showed that the M-POSSUM could accurately predict postoperative complications and mortality.CONCLUSION: M-POSSUM correlates well with postoperative complications and mortality, and is more accurate than POSSUM.
基金This research was funded by a VPR Special Research Grant entitled Potential of a Site-Specific DNA Interstrand Crosslink.
文摘As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learning method is presented for counting the colony forming units(CFUs),which is referred to as CFUCounter.This cellcounting program processes digital images and segments bacterial colonies.The algorithm combines unsupervised machine learning,iterative adaptive thresholding,and local-minima-based watershed segmentation to enable an accurate and robust cell counting.Compared to a manual counting method,CFUCounter supports color-based CFU classification,allows plates containing heterologous colonies to be counted individually,and demonstrates overall performance(slope 0.996,SD 0.013,95%CI:0.97–1.02,p value<1e-11,r=0.999)indistinguishable from the gold standard of point-and-click counting.This CFUCounter application is open-source and easy to use as a unique addition to the arsenal of colony-counting tools.
文摘Multiple-Input Multiple-Output (MIMO) techniques are promising in wireless communication systems for its high spectral efficiency. Sphere Detector (SD) is favoured in MIMO detection to achieve Maximum-Likelihood (ML) performance. In this paper, we proposed a new SD method for MIMO-Orthogonal Frequency Division Multiplexing (OFDM) systems based on IEEE802.11n, which uses Singular Value Decomposition (SVD) in complex domain to reduce the computation complexity. Furthermore, a new Schnorr-Euchner (SE) enumeration algorithm is also discussed in detail. The computer simulation result shows that the computational complexity and the number of visited nodes can be reduced significantly compared with conventional SD detectors with the same Bit Error Rate (BER) performance.
基金supported by the National Natural Science Foundation of China(No.20874088)
文摘The number of configurations, c（n, m）, of a single chain with length n attached to a flat surface with m monomers contacting the surface is exactly enumerated. A fimction of c（n, m） about m and n is obtained. From the function, a scaling law for mean energy of chain is derived, and we estimate the critical point εc = 0.276 and the crossover exponent φ = 0.5. The free energy difference between tethered chain and free chain in dilute solution is also studied, which shows the critical adsorption point is about 0.272 for infinite long chain with φ= 0.5.
基金supported by the National Natural Science Foundation of China (Grant Nos. 61877046, 12271419, and 62106186)the Natural Science Basic Research Program of Shaanxi (Program No. 2022JQ-620)the Fundamental Research Funds for the Central Universities (Grant Nos. XJS220709, JB210701, and QTZX23002)。
文摘The disintegration of networks is a widely researched topic with significant applications in fields such as counterterrorism and infectious disease control. While the traditional approaches for achieving network disintegration involve identifying critical sets of nodes or edges, limited research has been carried out on edge-based disintegration strategies. We propose a novel algorithm, i.e., a rank aggregation elite enumeration algorithm based on edge-coupled networks(RAEEC),which aims to implement tiling for edge-coupled networks by finding important sets of edges in the network while balancing effectiveness and efficiency. Our algorithm is based on a two-layer edge-coupled network model with one-to-one links, and utilizes three advanced edge importance metrics to rank the edges separately. A comprehensive ranking of edges is obtained using a rank aggregation approach proposed in this study. The top few edges from the ranking set obtained by RAEEC are then used to generate an enumeration set, which is continuously iteratively updated to identify the set of elite attack edges.We conduct extensive experiments on synthetic networks to evaluate the performance of our proposed method, and the results indicate that RAEEC achieves a satisfactory balance between efficiency and effectiveness. Our approach represents a significant contribution to the field of network disintegration, particularly for edge-based strategies.
基金Project supported by National Natural Foundation of China
文摘In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonian graphs. Some computational results obtained by microcomputers are listed.
文摘During the late incubation period or initial phase of dengue virus infection,laboratory confirmation is through viral isolation in cell culture and/or molecular investigations, or immunofluorescence,or immunohistochemistry[1].The dengue virus non-structural antigen NSl that would develop before the appearance of dengue IgM and/or IgG is emerging as a suitable option for dengue diagnosis[2].Platelet therapy is a standard clinical practice for dengue patients with severe thrombocytopenia[3].However,during introductory screening,platelet count is not being done in many cases. This results in delays of platelet therapy. In the course of the current(2010) spurt of dengue in New Delhi[4],simultaneous screening for NSl,IgM and IgG and platelet enumeration has been introduced at the
文摘The graphtheory method is used to enumerate the numbers of constitutions, configurations,chiral and achiral configurations of substituted crown ethers.The numerical results are tabulated.
文摘INTRODUCTION Fecal coliform has been used as an indicator of water pollution for about a century. Recently, Standrige et al (1981), Reasoner et al (1979) and Pagel et al. (1982) improved, with good results, the bioassy methods for fecal coliform in drinking water and waste water. To provide a method appli-
文摘A combinatorial method based on the determination of the averaged weight of permutations controlling the chirality/achirality fittingness of 2n substitution sites of the monocyclic cycloalkane allows to obtain generalized functional equations for direct enumeration of enantiomers pairs and achiral skeletons of any derivatives of monocyclic cycloalkanes having heteromorphic alkyl substituents with the distinct length k with the empirical formula , wherein at least two alkyl groups??of the distinct size ?each. ?is the number of alkyl radicals ?of the system??verifying the relation . The integer sequences of enantiomer pairs and achiral skeletons are given for substituted derivatives of monocyclic cycloalkane for n = 3, 4 and k = 3, 4, 5. The composite stereoisomerism of this particular compound is also highlighted.
文摘Simple formulas for the number of different cyclic and dihedral necklaces containing nj beads of the j-th color, and , are derived, using the Pólya enumeration theorem.
基金supported by National Health and Family Planning Commission of PRC
文摘Lactic acid bacteria （LAB） are widely used in food industries. Correct identification and safety evaluation of these bacteria at the species even strain level should take considerations into account. In this study, the LAB were recovered from yoghurt and characterized phenotypically and genetically. Fifty-two isolates of LAB from 31 yoghurt samples were cultured and grouped into 6 species including Luctobucillus bulguricus （24 isolates）, Streptococcus thermophilus （15 isolates）, L. ucidophilus （7 isolates）, L. porucusei/cusei （3 isolates）, L. delbrueckii （2 isolates）, and L. fermentum （1 isolate）, based on their Gram-staining, colony morphology and biochemical properties.
文摘A new combinatorial interpretation of Raney numbers is proposed. We apply this combinatorial interpretation to solve several tree enumeration counting problems. Further a generalized Catalan triangle is introduced and some of its properties are proved.
文摘The enumeration of elements of c.e. sets in the theory of computability and computational complexity has already been investigated. However, the order of this enumeration has received less attention. The enumeration orders of elements of c.e. sets by means of Turing machines on natural numbers are investigated. In this paper, we consider the enumeration orders of elements of c.e. sets on rational numbers. We present enumeration order reducibility and enumeration order equivalence on rational numbers and propose some lemmas and theorems on these concepts. Also, we show that the theories here hold for Rc and we could repeat the same theories in this domain, in a same way.
文摘A map is singular if each edge is on the same face on a surface (i.e., it has only one face on a surface). In this paper we present the chromatic enumeration for rooted singular maps on the Klein bottle.
