Totally three articles focusing on ＂molecular biological mechanism by which gene modification and RNA interference techniques interfere Parkinson’s disease and Alzheimer’s disease＂ are published in three issues. W...Totally three articles focusing on ＂molecular biological mechanism by which gene modification and RNA interference techniques interfere Parkinson’s disease and Alzheimer’s disease＂ are published in three issues. We hope that our readers find these papers useful to their research.展开更多
The thawing and warming of ice-rich permafrost present a considerable threat to the long-term stability of the Qinghai-Xizang Railway(QXR)on the roof of the world—that is,the Qinghai-Xizang Plateau(QXP).In this revie...The thawing and warming of ice-rich permafrost present a considerable threat to the long-term stability of the Qinghai-Xizang Railway(QXR)on the roof of the world—that is,the Qinghai-Xizang Plateau(QXP).In this review,we explore the extent of the observed permafrost degradation and embankment damage under the coupled impacts of climate change and engineering construction.The ice-rich permafrost beneath the embankment presents a substantial threat to the thermal-mechanical stability of the embankment due to the permafrost’s accelerated and amplified degradation.The observed embankment deformation has a potential high risk of thaw settlement,especially for 656 embankment-bridge sections,whose potential high risk of thaw settlement may be as great as 25%.Several techniques for roadbed cooling can be used to alleviate these impacts,including crushed rock structure embankments(CRSEs),thermosyphons,and reinforcement measures,which have been demonstrated to be successful in cooling the underlying permafrost and stabilizing an embankment.Under future climate change and permafrost degradation,however,the QXR still faces a high risk of embankment damage caused by permafrost degradation and requires more effective methods to reinforce the thermal-mechanical stability of permafrost.Therefore,a better understanding of such high-risk regions is needed,and roadbed cooling techniques will require further adaption in order to address the issues brought by climate change.展开更多
Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mech...Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mechanisms underlying the interactions between metastatic cells and the ECM, however, remain poorly understood, and the number of in vitro models available for the study of the peritoneal metastatic process is limited. Here, we show that decellularized ECM of the peritoneal cavity allows the growth of organoids obtained from PM, favoring the development of three-dimensional (3D) nodules that maintain the characteristics of in vivo PM. Organoids preferentially grow on scaffolds obtained from neoplastic peritoneum, which are characterized by greater stiffness than normal scaffolds. A gene expression analysis of organoids grown on different substrates reflected faithfully the clinical and biological characteristics of the organoids. An impact of the ECM on the response to standard chemotherapy treatment for PM was also observed. The ex vivo 3D model, obtained by combining patient-derived decellularized ECM with organoids to mimic the metastatic niche, could be an innovative tool to develop new therapeutic strategies in a biologically relevant context to personalize treatments.展开更多
文摘Totally three articles focusing on ＂molecular biological mechanism by which gene modification and RNA interference techniques interfere Parkinson’s disease and Alzheimer’s disease＂ are published in three issues. We hope that our readers find these papers useful to their research.
基金financially supported in part by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2021QZKK0205)the National Natural Science Foundation of China(42230512).
文摘The thawing and warming of ice-rich permafrost present a considerable threat to the long-term stability of the Qinghai-Xizang Railway(QXR)on the roof of the world—that is,the Qinghai-Xizang Plateau(QXP).In this review,we explore the extent of the observed permafrost degradation and embankment damage under the coupled impacts of climate change and engineering construction.The ice-rich permafrost beneath the embankment presents a substantial threat to the thermal-mechanical stability of the embankment due to the permafrost’s accelerated and amplified degradation.The observed embankment deformation has a potential high risk of thaw settlement,especially for 656 embankment-bridge sections,whose potential high risk of thaw settlement may be as great as 25%.Several techniques for roadbed cooling can be used to alleviate these impacts,including crushed rock structure embankments(CRSEs),thermosyphons,and reinforcement measures,which have been demonstrated to be successful in cooling the underlying permafrost and stabilizing an embankment.Under future climate change and permafrost degradation,however,the QXR still faces a high risk of embankment damage caused by permafrost degradation and requires more effective methods to reinforce the thermal-mechanical stability of permafrost.Therefore,a better understanding of such high-risk regions is needed,and roadbed cooling techniques will require further adaption in order to address the issues brought by climate change.
基金supported by an Italian law that allows taxpayers to allocate 0.5%of their tax to a research institution of their choice,by EU Horizon 2020 Marie Skłodowska-Curie programme 812772(project Phys2BioMed)by FET Open 801126(project EDIT).
文摘Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mechanisms underlying the interactions between metastatic cells and the ECM, however, remain poorly understood, and the number of in vitro models available for the study of the peritoneal metastatic process is limited. Here, we show that decellularized ECM of the peritoneal cavity allows the growth of organoids obtained from PM, favoring the development of three-dimensional (3D) nodules that maintain the characteristics of in vivo PM. Organoids preferentially grow on scaffolds obtained from neoplastic peritoneum, which are characterized by greater stiffness than normal scaffolds. A gene expression analysis of organoids grown on different substrates reflected faithfully the clinical and biological characteristics of the organoids. An impact of the ECM on the response to standard chemotherapy treatment for PM was also observed. The ex vivo 3D model, obtained by combining patient-derived decellularized ECM with organoids to mimic the metastatic niche, could be an innovative tool to develop new therapeutic strategies in a biologically relevant context to personalize treatments.
