Electrotaxis,a method that utilizes an electric field to direct the motion of particles or droplets,typically involves a droplet on a flat surface being guided by an electrically charged tweezer above it.Traditional e...Electrotaxis,a method that utilizes an electric field to direct the motion of particles or droplets,typically involves a droplet on a flat surface being guided by an electrically charged tweezer above it.Traditional electrotaxis methods have relied on voltages over 1.5 kV for droplet control,but this has several disadvantages,such as high voltage demands,the danger of electric discharge to the droplet,possible adhesion of the droplet to the tweezer,and issues with droplet oscillation and overshoot,which impede its broader application.The present study proposes an innovative tweezer design that not only operates at reduced voltages but also reduces droplet oscillation relative to traditional tweezers.This tweezer features a coaxial tubular sheath encircling the electrode,which modifies the electric field’s influence on the droplet.Numerical simulations were employed to obtain the tweezer’s ideal dimensions and shape.The empirical evidence indicates that the new tweezer can adeptly steer droplets at a markedly lower voltage of 620 V,ensuring a more stable trajectory and significantly diminishing overshoot.The tweezer’s distinctive design also decreases the possibility of electric discharge to the droplet,thus improving the safety of the system for managing delicate droplets.In the experiment,the maximum droplet translation speed attained was 105 mm/s at an applied voltage of 1.2 kV.This represents a 29.6%increase in speed and a 70%decrease in the required voltage compared to the previously highest reported droplet speed.展开更多
Purpose:This study aims to elucidate the electrotaxis response of alveolar epithelial cells(AECs)in direct-current electric fields(EFs),explore the impact of EFs on the cell fate of AECs,and lay the foundation for fut...Purpose:This study aims to elucidate the electrotaxis response of alveolar epithelial cells(AECs)in direct-current electric fields(EFs),explore the impact of EFs on the cell fate of AECs,and lay the foundation for future exploitation of EFs for the treatment of acute lung injury.Methods:AECs were extracted from rat lung tissues using magnetic-activated cell sorting.To elucidate the electrotaxis responses of AECs,different voltages of EFs(0,50,100,and 200 mV/mm)were applied to two types of AECs,respectively.Cell migrations were recorded and trajectories were pooled to better demonstrate cellular activities through graphs.Cell directionality was calculated as the cosine value of the angle formed by the EF vector and cell migration.To further demonstrate the impact of EFs on the pulmonary tissue,the human bronchial epithelial cells transformed with Ad12-SV402B(BEAS-2B cells)were obtained and experimented under the same conditions as AECs.To determine the influence on cell fate,cells underwent electric stimulation were collected to perform Western blot analysis.Results:The successful separation and culturing of AECs were confirmed through immunofluorescence staining.Compared with the control,AECs in EFs demonstrated a significant directionality in a voltage-dependent way.In general,type I alveolar epithelial cells migrated faster than type II alveolar epithelial cells,and under EFs,these two types of cells exhibited different response threshold.For type II alveolar epithelial cells,only EFs at 200 mV/mm resulted a significant difference to the velocity,whereas for,EFs at both 100 mV/mm and 200 mV/mm gave rise to a significant difference.Western blotting suggested that EFs led to an increased expression of a AKT and myeloid leukemia 1 and a decreased expression of Bcl-2-associated X protein and Bcl-2-like protein 11.Conclusion:EFs could guide and accelerate the directional migration of AECs and exert antiapoptotic effects,which indicated that EFs are important biophysical signals in the re-epithelialization of alveolar epithelium in lung injury.展开更多
Wound healing is a complex biological process that involves proliferation,migration,and differentiation.Endogenous electric field(EF)-directed migration of keratinocytes(galvanotaxis)is an essential step in wound re-e...Wound healing is a complex biological process that involves proliferation,migration,and differentiation.Endogenous electric field(EF)-directed migration of keratinocytes(galvanotaxis)is an essential step in wound re-epithelialization.Endogenous EFs are generated instantaneously after an injury because of the collapse of transepithelial potentials.The application of exogenous EF has become increasingly widespread in promoting wound healing,leading to a paradigm shift in patient outcomes.Here,we summarize the role and value of EF in wound healing through a review of the current research.展开更多
文摘Electrotaxis,a method that utilizes an electric field to direct the motion of particles or droplets,typically involves a droplet on a flat surface being guided by an electrically charged tweezer above it.Traditional electrotaxis methods have relied on voltages over 1.5 kV for droplet control,but this has several disadvantages,such as high voltage demands,the danger of electric discharge to the droplet,possible adhesion of the droplet to the tweezer,and issues with droplet oscillation and overshoot,which impede its broader application.The present study proposes an innovative tweezer design that not only operates at reduced voltages but also reduces droplet oscillation relative to traditional tweezers.This tweezer features a coaxial tubular sheath encircling the electrode,which modifies the electric field’s influence on the droplet.Numerical simulations were employed to obtain the tweezer’s ideal dimensions and shape.The empirical evidence indicates that the new tweezer can adeptly steer droplets at a markedly lower voltage of 620 V,ensuring a more stable trajectory and significantly diminishing overshoot.The tweezer’s distinctive design also decreases the possibility of electric discharge to the droplet,thus improving the safety of the system for managing delicate droplets.In the experiment,the maximum droplet translation speed attained was 105 mm/s at an applied voltage of 1.2 kV.This represents a 29.6%increase in speed and a 70%decrease in the required voltage compared to the previously highest reported droplet speed.
基金National Natural Science Foundation of China(82272908,81672287,82222038)Natural Science Foundation of Chongqing(CSTB2022NSCQ-MSX1110)+1 种基金Open Project Program of the State Key Laboratory of Trauma,Burn and Combined Injury(SKLYQ202102,SKLKF2022011)Daping Hospital of Army Medical University(2019CXJSB004,2019CXJSC024)。
文摘Purpose:This study aims to elucidate the electrotaxis response of alveolar epithelial cells(AECs)in direct-current electric fields(EFs),explore the impact of EFs on the cell fate of AECs,and lay the foundation for future exploitation of EFs for the treatment of acute lung injury.Methods:AECs were extracted from rat lung tissues using magnetic-activated cell sorting.To elucidate the electrotaxis responses of AECs,different voltages of EFs(0,50,100,and 200 mV/mm)were applied to two types of AECs,respectively.Cell migrations were recorded and trajectories were pooled to better demonstrate cellular activities through graphs.Cell directionality was calculated as the cosine value of the angle formed by the EF vector and cell migration.To further demonstrate the impact of EFs on the pulmonary tissue,the human bronchial epithelial cells transformed with Ad12-SV402B(BEAS-2B cells)were obtained and experimented under the same conditions as AECs.To determine the influence on cell fate,cells underwent electric stimulation were collected to perform Western blot analysis.Results:The successful separation and culturing of AECs were confirmed through immunofluorescence staining.Compared with the control,AECs in EFs demonstrated a significant directionality in a voltage-dependent way.In general,type I alveolar epithelial cells migrated faster than type II alveolar epithelial cells,and under EFs,these two types of cells exhibited different response threshold.For type II alveolar epithelial cells,only EFs at 200 mV/mm resulted a significant difference to the velocity,whereas for,EFs at both 100 mV/mm and 200 mV/mm gave rise to a significant difference.Western blotting suggested that EFs led to an increased expression of a AKT and myeloid leukemia 1 and a decreased expression of Bcl-2-associated X protein and Bcl-2-like protein 11.Conclusion:EFs could guide and accelerate the directional migration of AECs and exert antiapoptotic effects,which indicated that EFs are important biophysical signals in the re-epithelialization of alveolar epithelium in lung injury.
基金This work was supported by the National Nature Science Foundation of China(NSFC no.81873936).
文摘Wound healing is a complex biological process that involves proliferation,migration,and differentiation.Endogenous electric field(EF)-directed migration of keratinocytes(galvanotaxis)is an essential step in wound re-epithelialization.Endogenous EFs are generated instantaneously after an injury because of the collapse of transepithelial potentials.The application of exogenous EF has become increasingly widespread in promoting wound healing,leading to a paradigm shift in patient outcomes.Here,we summarize the role and value of EF in wound healing through a review of the current research.
